MCD1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	MCD1
Entrez Gene ID	851561
Full Name	kleisin alpha
Synonyms	PDS3,RHC21,SCC1
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001180062.1	NP_010281.1	kleisin alpha

Pathways from BioSystems

Homologies

Saccharomyces cerevisiae (baker's yeast)	MCD1	NP_010281.1
Eremothecium gossypii	AGOS_AEL007W	NP_984854.2
Kluyveromyces lactis	KLLA0F14795g	XP_455745.1

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.
Jacq C, Alt-M?rbe J, Andre B, Arnold W, Bahr A, Ballesta JP, Bargues M, Baron L, Becker A, Biteau N, Bl?cker H, Blugeon C, Boskovic J, Brandt P, Br?ckner M, Buitrago MJ, Coster F, Delaveau T, del Rey F, Dujon B, Eide LG, Garcia-Cantalejo JM, Goffeau A, Gomez-Peris A, Zaccaria P
Nature387(6632 Suppl)75-8(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

budding yeast Plk1, Cdc5, is pre-deposited onto cohesin engaged in cohesion on chromosome arms in G2/M phase cells. The Cdc5-cohesin association is mediated by direct interaction between the polo-box domain of Cdc5 and Scc1 phosphorylated at multiple sites in its middle region.
Title: Physical Association of Saccharomyces cerevisiae Polo-like Kinase Cdc5 with Chromosomal Cohesin Facilitates DNA Damage Response.

the integrity of the Pds5-Scc1 interface is indispensable for the recruitment of Pds5 to cohesin, and that its abrogation results in loss of sister chromatid cohesion and cell viability.
Title: Structure of the Pds5-Scc1 Complex and Implications for Cohesin Function.

Study shows that cohesin's release from the DNA is mediated by disengagement of the Smc3/Scc1 interface involving interaction between the SMC ATPase heads and is inhibited by Smc3 acetylation.
Title: Releasing Activity Disengages Cohesin's Smc3/Scc1 Interface in a Process Blocked by Acetylation.

Studied the function of Mcd1 in meiosis by ectopically expressing Mcd1 during early meiotic prophase I in Saccharomyces cerevisiae.
Title: Mitotic cohesin subunit Mcd1 regulates the progression of meiotic recombination in budding yeast.

We show that residues Y371 and K372 within the SCD are critical for viability and chromosome condensation but dispensable for cohesion. However, scc3 Y371A and scc3 K372A bind normally to Mcd1
Title: A conserved domain in the scc3 subunit of cohesin mediates the interaction with both mcd1 and the cohesin loader complex.

The following MCD1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MCD1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi01090	NM_001180062.1 Latest version!	Saccharomyces cerevisiae S288C kleisin alpha (MCD1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MCD1 ( NM_001180062.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_010281.1 Saccharomyces cerevisiae S288C kleisin alpha (MCD1), partial mRNA.

Price & Availability↑

CloneID	OSi01090
Clone ID Related Accession (Same CDS sequence)	NM_001180062.1
Accession Version	NM_001180062.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1701bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	kleisin alpha
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001136). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGGTTACAG AAAATCCTCA ACGTCTTACT GTTTTAAGAC TTGCCACCAA TAAGGGTCCA 
TTAGCACAAA TATGGCTTGC TTCAAATATG TCGAATATAC CAAGAGGATC GGTTATCCAA 
ACACATATTG CTGAATCAGC AAAGGAAATT GCAAAAGCTT CTGGCTGTGA TGACGAATCA 
GGGGACAATG AATACATAAC TTTACGAACA TCTGGCGAAT TACTTCAAGG CATTGTTCGT 
GTATATTCCA AACAGGCCAC TTTTCTCCTT ACAGATATAA AGGACACATT AACGAAGATA 
AGTATGTTAT TTAAAACAAG CCAGAAGATG ACTTCTACAG TAAACAGACT GAACACTGTA 
ACAAGGGTTC ATCAATTGAT GTTAGAAGAT GCCGTTACAG AAAGAGAAGT TTTGGTCACC 
CCAGGGTTAG AGTTCTTAGA TGATACTACT ATACCAGTGG GCTTAATGGC GCAGGAGAAT 
TCGATGGAAA GAAAAGTCCA AGGTGCTGCT CCCTGGGATA CTTCACTTGA GGTCGGTAGA 
CGATTCAGTC CAGATGAAGA TTTCGAACAT AATAATTTGT CTAGTATGAA CCTTGATTTT 
GACATCGAGG AAGGTCCGAT AACTTCAAAA TCTTGGGAAG AAGGAACAAG ACAAAGCTCA 
CGCAACTTTG ATACGCACGA AAATTACATA CAAGATGACG ATTTCCCTCT TGATGATGCT 
GGAACTATTG GTTGGGATCT AGGGATTACC GAAAAAAATG ACCAGAACAA CGATGATGAC 
GATAACTCTG TTGAGCAAGG AAGAAGGCTA GGTGAATCAA TCATGTCTGA GGAGCCTACG 
GATTTTGGAT TTGATCTGGA TATAGAAAAA GAAGCACCCG CAGGCAATAT AGATACGATA 
ACCGATGCCA TGACAGAATC ACAGCCAAAA CAAACTGGCA CAAGAAGGAA CTCAAAATTA 
CTGAATACCA AAAGTATTCA AATCGATGAA GAAACAGAAA ATTCAGAAAG CATAGCTTCA 
TCCAATACAT ACAAAGAGGA GAGATCGAAT AATTTATTGA CTCCACAGCC CACCAATTTT 
ACGACTAAAA GATTATGGAG TGAAATAACT GAAAGTATGT CCTATTTACC AGATCCAATC 
CTTAAGAACT TCTTATCCTA CGAATCTCTA AAAAAAAGAA AAATACATAA TGGGCGAGAA 
GGGTCAATTG AAGAACCTGA GTTAAACGTG TCGCTTAATC TTACCGACGA TGTAATTAGT 
AATGCGGGAA CAAACGATAA CTCCTTCAAT GAACTGACCG ATAACATGTC AGACTTCGTA 
CCAATTGATG CTGGCTTAAA TGAGGCTCCT TTTCCTGAAG AGAATATCAT CGACGCAAAG 
ACTAGAAATG AGCAAACTAC CATCCAAACT GAAAAAGTTC GTCCTACTCC CGGTGAGGTG 
GCTTCTAAAG CTATTGTTCA AATGGCGAAG ATTTTAAGGA AGGAATTAAG CGAAGAAAAA 
GAAGTAATAT TCACGGATGT ACTAAAAAGT CAAGCAAATA CAGAACCAGA AAATATTACT 
AAAAGAGAAG CCAGTAGAGG ATTTTTCGAT ATTTTATCAC TTGCTACAGA AGGATGTATT 
GGTTTGAGTC AAACAGAAGC ATTCGGAAAT ATTAAAATAG ACGCCAAACC TGCACTATTT 
GAAAGGTTTA TCAATGCTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_010281.1
CDS	1..1701
Translation	MVTENPQRLTVLRLATNKGPLAQIWLASNMSNIPRGSVIQTHIAESAKEIAKASGCDDESGDNEYITLRTSGELLQGIVRVYSKQATFLLTDIKDTLTKISMLFKTSQKMTSTVNRLNTVTRVHQLMLEDAVTEREVLVTPGLEFLDDTTIPVGLMAQENSMERKVQGAAPWDTSLEVGRRFSPDEDFEHNNLSSMNLDFDIEEGPITSKSWEEGTRQSSRNFDTHENYIQDDDFPLDDAGTIGWDLGITEKNDQNNDDDDNSVEQGRRLGESIMSEEPTDFGFDLDIEKEAPAGNIDTITDAMTESQPKQTGTRRNSKLLNTKSIQIDEETENSESIASSNTYKEERSNNLLTPQPTNFTTKRLWSEITESMSYLPDPILKNFLSYESLKKRKIHNGREGSIEEPELNVSLNLTDDVISNAGTNDNSFNELTDNMSDFVPIDAGLNEAPFPEENIIDAKTRNEQTTIQTEKVRPTPGEVASKAIVQMAKILRKELSEEKEVIFTDVLKSQANTEPENITKREASRGFFDILSLATEGCIGLSQTEAFGNIKIDAKPALFERFINA

Target ORF information:

RefSeq Version	NM_001180062.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C kleisin alpha (MCD1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001180062.1

ORF Insert Sequence:

ATGGTTACAG AAAATCCTCA ACGTCTTACT GTTTTAAGAC TTGCCACCAA TAAGGGTCCA 
TTAGCACAAA TATGGCTTGC TTCAAATATG TCGAATATAC CAAGAGGATC GGTTATCCAA 
ACACATATTG CTGAATCAGC AAAGGAAATT GCAAAAGCTT CTGGCTGTGA TGACGAATCA 
GGGGACAATG AATACATAAC TTTACGAACA TCTGGCGAAT TACTTCAAGG CATTGTTCGT 
GTATATTCCA AACAGGCCAC TTTTCTCCTT ACAGATATAA AGGACACATT AACGAAGATA 
AGTATGTTAT TTAAAACAAG CCAGAAGATG ACTTCTACAG TAAACAGACT GAACACTGTA 
ACAAGGGTTC ATCAATTGAT GTTAGAAGAT GCCGTTACAG AAAGAGAAGT TTTGGTCACC 
CCAGGGTTAG AGTTCTTAGA TGATACTACT ATACCAGTGG GCTTAATGGC GCAGGAGAAT 
TCGATGGAAA GAAAAGTCCA AGGTGCTGCT CCCTGGGATA CTTCACTTGA GGTCGGTAGA 
CGATTCAGTC CAGATGAAGA TTTCGAACAT AATAATTTGT CTAGTATGAA CCTTGATTTT 
GACATCGAGG AAGGTCCGAT AACTTCAAAA TCTTGGGAAG AAGGAACAAG ACAAAGCTCA 
CGCAACTTTG ATACGCACGA AAATTACATA CAAGATGACG ATTTCCCTCT TGATGATGCT 
GGAACTATTG GTTGGGATCT AGGGATTACC GAAAAAAATG ACCAGAACAA CGATGATGAC 
GATAACTCTG TTGAGCAAGG AAGAAGGCTA GGTGAATCAA TCATGTCTGA GGAGCCTACG 
GATTTTGGAT TTGATCTGGA TATAGAAAAA GAAGCACCCG CAGGCAATAT AGATACGATA 
ACCGATGCCA TGACAGAATC ACAGCCAAAA CAAACTGGCA CAAGAAGGAA CTCAAAATTA 
CTGAATACCA AAAGTATTCA AATCGATGAA GAAACAGAAA ATTCAGAAAG CATAGCTTCA 
TCCAATACAT ACAAAGAGGA GAGATCGAAT AATTTATTGA CTCCACAGCC CACCAATTTT 
ACGACTAAAA GATTATGGAG TGAAATAACT GAAAGTATGT CCTATTTACC AGATCCAATC 
CTTAAGAACT TCTTATCCTA CGAATCTCTA AAAAAAAGAA AAATACATAA TGGGCGAGAA 
GGGTCAATTG AAGAACCTGA GTTAAACGTG TCGCTTAATC TTACCGACGA TGTAATTAGT 
AATGCGGGAA CAAACGATAA CTCCTTCAAT GAACTGACCG ATAACATGTC AGACTTCGTA 
CCAATTGATG CTGGCTTAAA TGAGGCTCCT TTTCCTGAAG AGAATATCAT CGACGCAAAG 
ACTAGAAATG AGCAAACTAC CATCCAAACT GAAAAAGTTC GTCCTACTCC CGGTGAGGTG 
GCTTCTAAAG CTATTGTTCA AATGGCGAAG ATTTTAAGGA AGGAATTAAG CGAAGAAAAA 
GAAGTAATAT TCACGGATGT ACTAAAAAGT CAAGCAAATA CAGAACCAGA AAATATTACT 
AAAAGAGAAG CCAGTAGAGG ATTTTTCGAT ATTTTATCAC TTGCTACAGA AGGATGTATT 
GGTTTGAGTC AAACAGAAGC ATTCGGAAAT ATTAAAATAG ACGCCAAACC TGCACTATTT 
GAAAGGTTTA TCAATGCTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.
Nature387(6632 Suppl)75-8(1997 May)
Jacq C,Alt-M?rbe J,Andre B,Arnold W,Bahr A,Ballesta JP,Bargues M,Baron L,Becker A,Biteau N,Bl?cker H,Blugeon C,Boskovic J,Brandt P,Br?ckner M,Buitrago MJ,Coster F,Delaveau T,del Rey F,Dujon B,Eide LG,Garcia-Cantalejo JM,Goffeau A,Gomez-Peris A,Zaccaria P

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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MCD1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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