NUP84 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	NUP84
Entrez Gene ID	851441
Full Name	Nup84p
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001180175.1	NP_010167.1	Nup84p

Pathways from BioSystems

Homologies

Kluyveromyces lactis	KLLA0E09087g	XP_454359.1
Eremothecium gossypii	AGOS_ADL289C	NP_983807.1
Saccharomyces cerevisiae (baker's yeast)	NUP84	NP_010167.1

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.
Jacq C, Alt-M?rbe J, Andre B, Arnold W, Bahr A, Ballesta JP, Bargues M, Baron L, Becker A, Biteau N, Bl?cker H, Blugeon C, Boskovic J, Brandt P, Br?ckner M, Buitrago MJ, Coster F, Delaveau T, del Rey F, Dujon B, Eide LG, Garcia-Cantalejo JM, Goffeau A, Gomez-Peris A, Zaccaria P
Nature387(6632 Suppl)75-8(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The kinesin-14 motor protein complex (Cik1-Kar3) cooperates with chromatin remodellers to mediate interactions between subtelomeric double-strand breaks and the Nup84 nuclear pore complex to ensure cell survival via break-induced replication.
Title: Perinuclear tethers license telomeric DSBs for a broad kinesin- and NPC-dependent DNA repair process.

This study provides evidence for a new role of the Nup84 complex and a number of mRNA processing factors in transcription elongation that supports a connection of pre-mRNA processing and nuclear export with transcription elongation.
Title: A novel assay identifies transcript elongation roles for the Nup84 complex and RNA processing factors.

The heterotypic ACE1 interaction of Nup84 and Nup145C is analogous to the homotypic ACE1 interaction of Sec31 that forms COPII lattice edge elements and is inconsistent with the alternative 'fence-like' NPC model.
Title: Molecular architecture of the Nup84-Nup145C-Sec13 edge element in the nuclear pore complex lattice.

crystal structure of the heterotrimeric Sec13 x Nup145C x Nup84 complex, centerpiece of the heptamer, at 3.2-A resolution. Nup84 forms a U-shaped alpha-helical solenoid domain, topologically similar to two other members of the heptamer, Nup145C and Nup85
Title: Structure of a trimeric nucleoporin complex reveals alternate oligomerization states.

As mapping of the individual components in the Nup84 complex places Nup120 and Nup133 at opposite ends of the heptamer, findings indicate a head-to-tail arrangement of elongated Nup84 complexes into a ring structure.
Title: Structural and functional analysis of Nup120 suggests ring formation of the Nup84 complex.

The following NUP84 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NUP84 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi00990	NM_001180175.1 Latest version!	Saccharomyces cerevisiae S288C Nup84p (NUP84), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NUP84 ( NM_001180175.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_010167.1 Saccharomyces cerevisiae S288C Nup84p (NUP84), partial mRNA.

Price & Availability↑

CloneID	OSi00990
Clone ID Related Accession (Same CDS sequence)	NM_001180175.1
Accession Version	NM_001180175.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2181bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	Nup84p
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001136). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGGAATTAT CCCCTACTTA TCAAACAGAA CGCTTCACAA AGTTCTCAGA CACTTTAAAG 
GAATTCAAAA TTGAACAAAA TAATGAGCAA AACCCCATTG ACCCGTTTAA CATCATAAGA 
GAGTTTCGCT CAGCCGCGGG GCAGCTTGCG TTAGATTTGG CTAATTCTGG AGATGAAAGT 
AACGTCATAT CTTCTAAGGA TTGGGAACTG GAAGCTAGAT TTTGGCATTT AGTAGAACTG 
TTACTGGTTT TCAGGAACGC TGATCTTGAT CTAGATGAAA TGGAACTTCA TCCTTATAAC 
TCGAGGGGTT TATTTGAAAA GAAATTAATG CAAGACAACA AACAACTTTA TCAAATATGG 
ATAGTGATGG TTTGGCTCAA AGAAAATACA TATGTGATGG AAAGACCCAA AAATGTACCA 
ACTTCAAAAT GGTTAAATAG TATTACTTCT GGAGGATTGA AAAGCTGTGA CTTGGATTTT 
CCTTTACGAG AAAACACTAA TGTCCTCGAT GTTAAAGATA AAGAAGAAGA TCATATTTTC 
TTCAAATACA TTTACGAACT AATTTTGGCC GGTGCGATTG ATGAAGCATT AGAAGAAGCT 
AAACTTTCCG ACAATATTTC AATTTGTATG ATCCTGTGTG GGATACAAGA GTACTTAAAC 
CCTGTCATTG ATACGCAAAT TGCAAACGAA TTCAATACCC AGCAAGGTAT CAAGAAGCAC 
TCTTTGTGGA GAAGAACAGT TTACAGCCTT TCGCAGCAGG CCGGTTTGGA CCCTTATGAA 
AGGGCGATAT ACAGCTATTT GAGTGGCGCT ATTCCCAACC AAGAAGTTCT GCAATATTCG 
GATTGGGAGT CTGATCTGCA CATACACTTA AATCAAATTT TACAAACTGA AATTGAAAAT 
TATCTTTTGG AAAACAATCA AGTAGGTACT GATGAACTGA TTTTACCATT ACCTTCACAC 
GCGCTAACTG TACAAGAGGT GCTTAATAGA GTGGCTTCAA GGCATCCCTC AGAAAGTGAA 
CATCCAATCA GAGTTTTAAT GGCATCTGTC ATACTAGATT CTTTGCCATC AGTTATTCAT 
TCATCCGTGG AAATGTTGCT TGATGTCGTC AAGGGAACCG AAGCTAGTAA CGATATTATA 
GATAAGCCTT ATCTTCTGAG GATAGTTACA CATTTAGCCA TTTGCCTTGA TATAATTAAT 
CCAGGTTCTG TTGAAGAGGT TGATAAGTCC AAGCTCATTA CTACGTATAT CAGTCTTCTG 
AAATTGCAAG GCCTTTATGA AAATATTCCA ATATATGCTA CTTTCCTGAA TGAGTCAGAC 
TGCTTAGAGG CATGCTCTTT CATATTGTCT TCTTTAGAGG ACCCCCAAGT AAGGAAAAAG 
CAAATTGAGA CAATAAACTT TCTAAGATTA CCTGCCTCAA ATATTTTGAG AAGAACCACA 
CAGCGTGTTT TCGATGAAAC CGAACAAGAA TATTCACCAT CTAACGAAAT TTCCATATCT 
TTCGATGTAA ACAACATTGA TATGCACTTA ATATATGGCG TGGAATGGCT TATTGAAGGG 
AAGCTTTATG TGGACGCTGT CCACTCTATC ATTGCCTTAT CGAGGAGATT TTTGTTAAAT 
GGCCGCGTAA AGGCTCTCGA ACAATTTATG GAGAGAAATA ATATTGGAGA AATATGCAAG 
AATTATGAAT TAGAGAAAAT AGCCGATAAC ATATCAAAGG ATGAAAATGA AGATCAGTTT 
TTGGAAGAAA TAACTCAATA TGAGCATTTG ATAAAGGGCA TAAGAGAATA TGAAGAATGG 
CAAAAATCCG TTAGCTTGTT AAGCTCTGAA TCGAACATTC CTACTTTAAT AGAGAAATTG 
CAAGGTTTTT CAAAGGATAC ATTTGAATTG ATCAAGACAT TTTTAGTAGA TTTAACTTCC 
TCTAATTTCG CGGATTCGGC AGATTATGAA ATCCTGTATG AGATTAGAGC CCTATATACT 
CCATTTCTAC TGATGGAATT GCATAAAAAG TTAGTGGAAG CCGCAAAGTT GTTAAAGATC 
CCAAAATTTA TTTCGGAAGC CCTGGCATTT ACCTCATTGG TCGCTAATGA GAACGACAAG 
ATTTATTTAT TATTCCAATC TAGTGGAAAG TTAAAAGAGT ATCTGGATCT CGTTGCTCGC 
ACAGCAACCC TTTCGAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_010167.1
CDS	1..2181
Translation	MELSPTYQTERFTKFSDTLKEFKIEQNNEQNPIDPFNIIREFRSAAGQLALDLANSGDESNVISSKDWELEARFWHLVELLLVFRNADLDLDEMELHPYNSRGLFEKKLMQDNKQLYQIWIVMVWLKENTYVMERPKNVPTSKWLNSITSGGLKSCDLDFPLRENTNVLDVKDKEEDHIFFKYIYELILAGAIDEALEEAKLSDNISICMILCGIQEYLNPVIDTQIANEFNTQQGIKKHSLWRRTVYSLSQQAGLDPYERAIYSYLSGAIPNQEVLQYSDWESDLHIHLNQILQTEIENYLLENNQVGTDELILPLPSHALTVQEVLNRVASRHPSESEHPIRVLMASVILDSLPSVIHSSVEMLLDVVKGTEASNDIIDKPYLLRIVTHLAICLDIINPGSVEEVDKSKLITTYISLLKLQGLYENIPIYATFLNESDCLEACSFILSSLEDPQVRKKQIETINFLRLPASNILRRTTQRVFDETEQEYSPSNEISISFDVNNIDMHLIYGVEWLIEGKLYVDAVHSIIALSRRFLLNGRVKALEQFMERNNIGEICKNYELEKIADNISKDENEDQFLEEITQYEHLIKGIREYEEWQKSVSLLSSESNIPTLIEKLQGFSKDTFELIKTFLVDLTSSNFADSADYEILYEIRALYTPFLLMELHKKLVEAAKLLKIPKFISEALAFTSLVANENDKIYLLFQSSGKLKEYLDLVARTATLSN

Target ORF information:

RefSeq Version	NM_001180175.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C Nup84p (NUP84), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001180175.1

ORF Insert Sequence:

ATGGAATTAT CCCCTACTTA TCAAACAGAA CGCTTCACAA AGTTCTCAGA CACTTTAAAG 
GAATTCAAAA TTGAACAAAA TAATGAGCAA AACCCCATTG ACCCGTTTAA CATCATAAGA 
GAGTTTCGCT CAGCCGCGGG GCAGCTTGCG TTAGATTTGG CTAATTCTGG AGATGAAAGT 
AACGTCATAT CTTCTAAGGA TTGGGAACTG GAAGCTAGAT TTTGGCATTT AGTAGAACTG 
TTACTGGTTT TCAGGAACGC TGATCTTGAT CTAGATGAAA TGGAACTTCA TCCTTATAAC 
TCGAGGGGTT TATTTGAAAA GAAATTAATG CAAGACAACA AACAACTTTA TCAAATATGG 
ATAGTGATGG TTTGGCTCAA AGAAAATACA TATGTGATGG AAAGACCCAA AAATGTACCA 
ACTTCAAAAT GGTTAAATAG TATTACTTCT GGAGGATTGA AAAGCTGTGA CTTGGATTTT 
CCTTTACGAG AAAACACTAA TGTCCTCGAT GTTAAAGATA AAGAAGAAGA TCATATTTTC 
TTCAAATACA TTTACGAACT AATTTTGGCC GGTGCGATTG ATGAAGCATT AGAAGAAGCT 
AAACTTTCCG ACAATATTTC AATTTGTATG ATCCTGTGTG GGATACAAGA GTACTTAAAC 
CCTGTCATTG ATACGCAAAT TGCAAACGAA TTCAATACCC AGCAAGGTAT CAAGAAGCAC 
TCTTTGTGGA GAAGAACAGT TTACAGCCTT TCGCAGCAGG CCGGTTTGGA CCCTTATGAA 
AGGGCGATAT ACAGCTATTT GAGTGGCGCT ATTCCCAACC AAGAAGTTCT GCAATATTCG 
GATTGGGAGT CTGATCTGCA CATACACTTA AATCAAATTT TACAAACTGA AATTGAAAAT 
TATCTTTTGG AAAACAATCA AGTAGGTACT GATGAACTGA TTTTACCATT ACCTTCACAC 
GCGCTAACTG TACAAGAGGT GCTTAATAGA GTGGCTTCAA GGCATCCCTC AGAAAGTGAA 
CATCCAATCA GAGTTTTAAT GGCATCTGTC ATACTAGATT CTTTGCCATC AGTTATTCAT 
TCATCCGTGG AAATGTTGCT TGATGTCGTC AAGGGAACCG AAGCTAGTAA CGATATTATA 
GATAAGCCTT ATCTTCTGAG GATAGTTACA CATTTAGCCA TTTGCCTTGA TATAATTAAT 
CCAGGTTCTG TTGAAGAGGT TGATAAGTCC AAGCTCATTA CTACGTATAT CAGTCTTCTG 
AAATTGCAAG GCCTTTATGA AAATATTCCA ATATATGCTA CTTTCCTGAA TGAGTCAGAC 
TGCTTAGAGG CATGCTCTTT CATATTGTCT TCTTTAGAGG ACCCCCAAGT AAGGAAAAAG 
CAAATTGAGA CAATAAACTT TCTAAGATTA CCTGCCTCAA ATATTTTGAG AAGAACCACA 
CAGCGTGTTT TCGATGAAAC CGAACAAGAA TATTCACCAT CTAACGAAAT TTCCATATCT 
TTCGATGTAA ACAACATTGA TATGCACTTA ATATATGGCG TGGAATGGCT TATTGAAGGG 
AAGCTTTATG TGGACGCTGT CCACTCTATC ATTGCCTTAT CGAGGAGATT TTTGTTAAAT 
GGCCGCGTAA AGGCTCTCGA ACAATTTATG GAGAGAAATA ATATTGGAGA AATATGCAAG 
AATTATGAAT TAGAGAAAAT AGCCGATAAC ATATCAAAGG ATGAAAATGA AGATCAGTTT 
TTGGAAGAAA TAACTCAATA TGAGCATTTG ATAAAGGGCA TAAGAGAATA TGAAGAATGG 
CAAAAATCCG TTAGCTTGTT AAGCTCTGAA TCGAACATTC CTACTTTAAT AGAGAAATTG 
CAAGGTTTTT CAAAGGATAC ATTTGAATTG ATCAAGACAT TTTTAGTAGA TTTAACTTCC 
TCTAATTTCG CGGATTCGGC AGATTATGAA ATCCTGTATG AGATTAGAGC CCTATATACT 
CCATTTCTAC TGATGGAATT GCATAAAAAG TTAGTGGAAG CCGCAAAGTT GTTAAAGATC 
CCAAAATTTA TTTCGGAAGC CCTGGCATTT ACCTCATTGG TCGCTAATGA GAACGACAAG 
ATTTATTTAT TATTCCAATC TAGTGGAAAG TTAAAAGAGT ATCTGGATCT CGTTGCTCGC 
ACAGCAACCC TTTCGAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.
Nature387(6632 Suppl)75-8(1997 May)
Jacq C,Alt-M?rbe J,Andre B,Arnold W,Bahr A,Ballesta JP,Bargues M,Baron L,Becker A,Biteau N,Bl?cker H,Blugeon C,Boskovic J,Brandt P,Br?ckner M,Buitrago MJ,Coster F,Delaveau T,del Rey F,Dujon B,Eide LG,Garcia-Cantalejo JM,Goffeau A,Gomez-Peris A,Zaccaria P

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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NUP84 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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