CDC48 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	CDC48
Entrez Gene ID	851431
Full Name	AAA family ATPase CDC48
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001180185.1	NP_010157.1	AAA family ATPase CDC48

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	VCP	XP_003312111.1
Gallus gallus (chicken)	VCP	NP_001038129.1
Magnaporthe oryzae (rice blast fungus)	MGG_05193	XP_003712735.1
Canis lupus familiaris (dog)	VCP	XP_852626.1
Bos taurus (cattle)	VCP	NP_001029466.1
Mus musculus (house mouse)	Vcp	NP_033529.3
Drosophila melanogaster (fruit fly)	TER94	NP_477369.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005630	XP_315644.3
Saccharomyces cerevisiae (baker's yeast)	CDC48	NP_010157.1
Schizosaccharomyces pombe (fission yeast)	cdc48	NP_593287.2
Neurospora crassa	NCU00018	XP_957005.2
Arabidopsis thaliana (thale cress)	AT3G53230	NP_190891.1
Rattus norvegicus (Norway rat)	Vcp	NP_446316.1
Danio rerio (zebrafish)	vcp	NP_958889.1
Kluyveromyces lactis	KLLA0F05676g	XP_455337.1
Arabidopsis thaliana (thale cress)	AT5G03340	NP_568114.1
Oryza sativa (rice)	Os03g0151800	NP_001048987.1
Xenopus tropicalis (tropical clawed frog)	vcp	NP_001005677.1
Caenorhabditis elegans (roundworm)	cdc-48.1	NP_496273.1
Caenorhabditis elegans (roundworm)	cdc-48.2	NP_495705.1
Arabidopsis thaliana (thale cress)	CDC48	NP_187595.1
Homo sapiens (human)	VCP	NP_009057.1
Eremothecium gossypii	AGOS_AFR158W	NP_985705.2

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.
Jacq C, Alt-M?rbe J, Andre B, Arnold W, Bahr A, Ballesta JP, Bargues M, Baron L, Becker A, Biteau N, Bl?cker H, Blugeon C, Boskovic J, Brandt P, Br?ckner M, Buitrago MJ, Coster F, Delaveau T, del Rey F, Dujon B, Eide LG, Garcia-Cantalejo JM, Goffeau A, Gomez-Peris A, Zaccaria P
Nature387(6632 Suppl)75-8(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Identify the Cdc48-Rad23/Dsk2 axis as a major route to the proteasome.
Title: In?Vivo Ubiquitin Linkage-type Analysis Reveals that the Cdc48-Rad23/Dsk2 Axis Contributes to K48-Linked Chain Specificity of the Proteasome.

Study elucidated the mechanism of Cdc48 from S. cerevisiae with purified components that include Cdc48 itself, its UN cofactor, and model substrates bearing K48-linked polyubiquitin chains.
Title: Molecular Mechanism of Substrate Processing by the Cdc48 ATPase Complex.

Multispanning endoplasmic reticulum membrane proteins, called ERAD-M substrates, are retrotranslocated to the cytosol as full-length intermediates during Endoplasmic Reticulum-associated Degradation, and this study investigated how they maintain substrate solubility. the Cdc48 complex is generally and critically needed for the solubility of retrotranslocated ERAD-M intermediates.
Title: A Cdc48 "Retrochaperone" Function Is Required for the Solubility of Retrotranslocated, Integral Membrane Endoplasmic Reticulum-associated Degradation (ERAD-M) Substrates.

Data show that Saccharomyces cerevisiae proteins Rqc1, Ltn1, and Cdc48 prevent the aggregation of aberrant proteins that can only appear after C-terminal alanine-threonine tail (CAT-tail) addition by Rqc2.
Title: Rqc1 and Ltn1 Prevent C-terminal Alanine-Threonine Tail (CAT-tail)-induced Protein Aggregation by Efficient Recruitment of Cdc48 on Stalled 60S Subunits.

Upon DNA damage Wss1/Cdc48/Doa1 ternary complex is recruited to sumoylated targets and catalyzes SUMO chain extension through a newly recognized SUMO ligase activity.
Title: Wss1 metalloprotease partners with Cdc48/Doa1 in processing genotoxic SUMO conjugates.

The following CDC48 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CDC48 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi00981	NM_001180185.1 Latest version!	Saccharomyces cerevisiae S288C AAA family ATPase CDC48 (CDC48), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CDC48 ( NM_001180185.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_010157.1 Saccharomyces cerevisiae S288C AAA family ATPase CDC48 (CDC48), partial mRNA.

Price & Availability↑

CloneID	OSi00981
Clone ID Related Accession (Same CDS sequence)	NM_001180185.1
Accession Version	NM_001180185.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2508bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	AAA family ATPase CDC48
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001136). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGGGTGAAG AACATAAACC ACTTTTGGAC GCCTCGGGTG TCGACCCTCG TGAGGAGGAT 
AAAACCGCCA CCGCCATTTT AAGAAGAAAG AAGAAGGACA ACATGCTTTT GGTGGACGAT 
GCTATCAATG ATGACAATTC TGTCATTGCT ATTAATTCTA ACACCATGGA CAAATTGGAA 
TTGTTCCGTG GTGACACCGT TCTCGTTAAG GGCAAGAAGA GAAAAGATAC TGTCTTAATT 
GTGTTGATCG ATGACGAATT AGAAGACGGA GCATGCAGGA TAAACCGTGT AGTTCGTAAC 
AATTTACGTA TTAGGCTGGG TGATTTAGTT ACAATTCATC CTTGCCCCGA TATCAAATAC 
GCTACTAGAA TTTCTGTGTT ACCAATTGCT GATACGATCG AAGGTATAAC TGGTAATCTT 
TTCGATGTTT TTTTGAAGCC TTATTTTGTG GAAGCCTACA GACCAGTGAG GAAAGGCGAC 
CATTTTGTTG TCAGAGGCGG TATGAGACAA GTCGAATTCA AGGTTGTGGA TGTCGAACCT 
GAAGAATATG CCGTCGTTGC TCAGGATACT ATTATTCACT GGGAAGGTGA GCCAATCAAC 
AGGGAAGATG AAGAAAATAA TATGAATGAG GTGGGTTACG ATGATATTGG TGGTTGTCGT 
AAGCAAATGG CTCAAATTAG GGAAATGGTT GAACTACCAT TGAGACATCC TCAGTTGTTC 
AAGGCTATCG GTATCAAGCC ACCAAGAGGT GTTTTGATGT ATGGTCCCCC TGGTACTGGT 
AAAACCTTGA TGGCAAGAGC TGTTGCTAAT GAGACCGGTG CCTTTTTTTT CTTAATTAAT 
GGTCCAGAAG TCATGTCAAA AATGGCTGGT GAATCAGAGT CTAACTTAAG AAAGGCCTTT 
GAAGAAGCCG AAAAGAACGC TCCAGCCATC ATTTTTATCG ATGAAATCGA TTCTATAGCT 
CCAAAGAGAG ACAAGACTAA TGGTGAAGTT GAGAGAAGAG TTGTCTCTCA ATTGTTAACT 
TTAATGGATG GTATGAAGGC AAGATCTAAT GTTGTTGTCA TTGCTGCTAC CAATAGACCA 
AATTCGATTG ATCCCGCTTT AAGAAGATTT GGTAGATTCG ATCGTGAAGT CGACATTGGT 
ATCCCAGATG CTACAGGTAG ACTTGAAGTT CTACGTATTC ACACCAAGAA CATGAAGTTG 
GCTGATGATG TTGATTTGGA AGCATTGGCT GCTGAAACAC ACGGTTACGT GGGTGCTGAT 
ATTGCCTCTT TGTGTTCTGA AGCTGCCATG CAACAGATCC GTGAAAAAAT GGACTTGATA 
GATTTAGATG AAGATGAAAT TGATGCAGAG GTGCTCGATT CTTTAGGAGT CACCATGGAC 
AACTTCAGAT TCGCATTGGG CAATTCAAAC CCATCTGCAT TGCGTGAAAC CGTTGTTGAA 
AGTGTCAATG TTACTTGGGA TGACGTTGGT GGCCTAGATG AAATCAAGGA GGAATTGAAG 
GAAACTGTAG AATACCCAGT CTTACATCCA GACCAATACA CAAAGTTTGG GTTATCACCA 
TCAAAGGGTG TGTTGTTCTA CGGTCCACCA GGTACAGGTA AAACTTTGTT AGCAAAAGCT 
GTAGCTACTG AGGTTTCTGC CAACTTTATC TCAGTCAAAG GTCCAGAATT ATTGAGTATG 
TGGTATGGTG AATCGGAATC TAACATCCGT GATATTTTTG ATAAGGCAAG AGCAGCTGCA 
CCAACCGTCG TTTTTCTAGA TGAATTAGAC TCCATTGCCA AGGCAAGAGG TGGTTCCTTG 
GGTGATGCTG GTGGTGCTTC GGATAGAGTC GTCAACCAAC TATTAACAGA AATGGATGGT 
ATGAATGCTA AGAAAAATGT GTTCGTGATT GGTGCCACCA ATAGGCCAGA TCAAATTGAT 
CCTGCTATTT TAAGACCTGG CAGATTGGAT CAATTAATTT ATGTTCCATT GCCAGATGAA 
AACGCTAGAT TATCTATTCT AAATGCTCAA TTAAGGAAAA CACCACTAGA ACCAGGGCTA 
GAGTTAACTG CGATTGCCAA GGCCACTCAA GGTTTCTCTG GTGCTGATTT ACTCTATATT 
GTGCAGAGGG CTGCTAAATA CGCTATCAAG GATTCTATCG AAGCTCACAG ACAACACGAA 
GCTGAAAAGG AAGTGAAAGT AGAAGGTGAA GACGTGGAAA TGACAGATGA GGGAGCAAAG 
GCTGAACAAG AACCAGAAGT AGATCCTGTT CCATATATTA CAAAGGAGCA TTTCGCAGAA 
GCTATGAAGA CCGCTAAACG TTCTGTTTCA GATGCTGAAT TGCGTCGTTA TGAAGCATAC 
TCACAGCAAA TGAAAGCATC TAGAGGTCAA TTCAGTAATT TCAACTTTAA TGACGCTCCA 
TTAGGTACTA CGGCTACGGA CAATGCTAAC AGTAATAATA GTGCTCCAAG TGGAGCAGGT 
GCTGCATTTG GTTCTAATGC GGAGGAAGAT GATGATTTGT ATAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_010157.1
CDS	1..2508
Translation	MGEEHKPLLDASGVDPREEDKTATAILRRKKKDNMLLVDDAINDDNSVIAINSNTMDKLELFRGDTVLVKGKKRKDTVLIVLIDDELEDGACRINRVVRNNLRIRLGDLVTIHPCPDIKYATRISVLPIADTIEGITGNLFDVFLKPYFVEAYRPVRKGDHFVVRGGMRQVEFKVVDVEPEEYAVVAQDTIIHWEGEPINREDEENNMNEVGYDDIGGCRKQMAQIREMVELPLRHPQLFKAIGIKPPRGVLMYGPPGTGKTLMARAVANETGAFFFLINGPEVMSKMAGESESNLRKAFEEAEKNAPAIIFIDEIDSIAPKRDKTNGEVERRVVSQLLTLMDGMKARSNVVVIAATNRPNSIDPALRRFGRFDREVDIGIPDATGRLEVLRIHTKNMKLADDVDLEALAAETHGYVGADIASLCSEAAMQQIREKMDLIDLDEDEIDAEVLDSLGVTMDNFRFALGNSNPSALRETVVESVNVTWDDVGGLDEIKEELKETVEYPVLHPDQYTKFGLSPSKGVLFYGPPGTGKTLLAKAVATEVSANFISVKGPELLSMWYGESESNIRDIFDKARAAAPTVVFLDELDSIAKARGGSLGDAGGASDRVVNQLLTEMDGMNAKKNVFVIGATNRPDQIDPAILRPGRLDQLIYVPLPDENARLSILNAQLRKTPLEPGLELTAIAKATQGFSGADLLYIVQRAAKYAIKDSIEAHRQHEAEKEVKVEGEDVEMTDEGAKAEQEPEVDPVPYITKEHFAEAMKTAKRSVSDAELRRYEAYSQQMKASRGQFSNFNFNDAPLGTTATDNANSNNSAPSGAGAAFGSNAEEDDDLYS

Target ORF information:

RefSeq Version	NM_001180185.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C AAA family ATPase CDC48 (CDC48), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001180185.1

ORF Insert Sequence:

ATGGGTGAAG AACATAAACC ACTTTTGGAC GCCTCGGGTG TCGACCCTCG TGAGGAGGAT 
AAAACCGCCA CCGCCATTTT AAGAAGAAAG AAGAAGGACA ACATGCTTTT GGTGGACGAT 
GCTATCAATG ATGACAATTC TGTCATTGCT ATTAATTCTA ACACCATGGA CAAATTGGAA 
TTGTTCCGTG GTGACACCGT TCTCGTTAAG GGCAAGAAGA GAAAAGATAC TGTCTTAATT 
GTGTTGATCG ATGACGAATT AGAAGACGGA GCATGCAGGA TAAACCGTGT AGTTCGTAAC 
AATTTACGTA TTAGGCTGGG TGATTTAGTT ACAATTCATC CTTGCCCCGA TATCAAATAC 
GCTACTAGAA TTTCTGTGTT ACCAATTGCT GATACGATCG AAGGTATAAC TGGTAATCTT 
TTCGATGTTT TTTTGAAGCC TTATTTTGTG GAAGCCTACA GACCAGTGAG GAAAGGCGAC 
CATTTTGTTG TCAGAGGCGG TATGAGACAA GTCGAATTCA AGGTTGTGGA TGTCGAACCT 
GAAGAATATG CCGTCGTTGC TCAGGATACT ATTATTCACT GGGAAGGTGA GCCAATCAAC 
AGGGAAGATG AAGAAAATAA TATGAATGAG GTGGGTTACG ATGATATTGG TGGTTGTCGT 
AAGCAAATGG CTCAAATTAG GGAAATGGTT GAACTACCAT TGAGACATCC TCAGTTGTTC 
AAGGCTATCG GTATCAAGCC ACCAAGAGGT GTTTTGATGT ATGGTCCCCC TGGTACTGGT 
AAAACCTTGA TGGCAAGAGC TGTTGCTAAT GAGACCGGTG CCTTTTTTTT CTTAATTAAT 
GGTCCAGAAG TCATGTCAAA AATGGCTGGT GAATCAGAGT CTAACTTAAG AAAGGCCTTT 
GAAGAAGCCG AAAAGAACGC TCCAGCCATC ATTTTTATCG ATGAAATCGA TTCTATAGCT 
CCAAAGAGAG ACAAGACTAA TGGTGAAGTT GAGAGAAGAG TTGTCTCTCA ATTGTTAACT 
TTAATGGATG GTATGAAGGC AAGATCTAAT GTTGTTGTCA TTGCTGCTAC CAATAGACCA 
AATTCGATTG ATCCCGCTTT AAGAAGATTT GGTAGATTCG ATCGTGAAGT CGACATTGGT 
ATCCCAGATG CTACAGGTAG ACTTGAAGTT CTACGTATTC ACACCAAGAA CATGAAGTTG 
GCTGATGATG TTGATTTGGA AGCATTGGCT GCTGAAACAC ACGGTTACGT GGGTGCTGAT 
ATTGCCTCTT TGTGTTCTGA AGCTGCCATG CAACAGATCC GTGAAAAAAT GGACTTGATA 
GATTTAGATG AAGATGAAAT TGATGCAGAG GTGCTCGATT CTTTAGGAGT CACCATGGAC 
AACTTCAGAT TCGCATTGGG CAATTCAAAC CCATCTGCAT TGCGTGAAAC CGTTGTTGAA 
AGTGTCAATG TTACTTGGGA TGACGTTGGT GGCCTAGATG AAATCAAGGA GGAATTGAAG 
GAAACTGTAG AATACCCAGT CTTACATCCA GACCAATACA CAAAGTTTGG GTTATCACCA 
TCAAAGGGTG TGTTGTTCTA CGGTCCACCA GGTACAGGTA AAACTTTGTT AGCAAAAGCT 
GTAGCTACTG AGGTTTCTGC CAACTTTATC TCAGTCAAAG GTCCAGAATT ATTGAGTATG 
TGGTATGGTG AATCGGAATC TAACATCCGT GATATTTTTG ATAAGGCAAG AGCAGCTGCA 
CCAACCGTCG TTTTTCTAGA TGAATTAGAC TCCATTGCCA AGGCAAGAGG TGGTTCCTTG 
GGTGATGCTG GTGGTGCTTC GGATAGAGTC GTCAACCAAC TATTAACAGA AATGGATGGT 
ATGAATGCTA AGAAAAATGT GTTCGTGATT GGTGCCACCA ATAGGCCAGA TCAAATTGAT 
CCTGCTATTT TAAGACCTGG CAGATTGGAT CAATTAATTT ATGTTCCATT GCCAGATGAA 
AACGCTAGAT TATCTATTCT AAATGCTCAA TTAAGGAAAA CACCACTAGA ACCAGGGCTA 
GAGTTAACTG CGATTGCCAA GGCCACTCAA GGTTTCTCTG GTGCTGATTT ACTCTATATT 
GTGCAGAGGG CTGCTAAATA CGCTATCAAG GATTCTATCG AAGCTCACAG ACAACACGAA 
GCTGAAAAGG AAGTGAAAGT AGAAGGTGAA GACGTGGAAA TGACAGATGA GGGAGCAAAG 
GCTGAACAAG AACCAGAAGT AGATCCTGTT CCATATATTA CAAAGGAGCA TTTCGCAGAA 
GCTATGAAGA CCGCTAAACG TTCTGTTTCA GATGCTGAAT TGCGTCGTTA TGAAGCATAC 
TCACAGCAAA TGAAAGCATC TAGAGGTCAA TTCAGTAATT TCAACTTTAA TGACGCTCCA 
TTAGGTACTA CGGCTACGGA CAATGCTAAC AGTAATAATA GTGCTCCAAG TGGAGCAGGT 
GCTGCATTTG GTTCTAATGC GGAGGAAGAT GATGATTTGT ATAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.
Nature387(6632 Suppl)75-8(1997 May)
Jacq C,Alt-M?rbe J,Andre B,Arnold W,Bahr A,Ballesta JP,Bargues M,Baron L,Becker A,Biteau N,Bl?cker H,Blugeon C,Boskovic J,Brandt P,Br?ckner M,Buitrago MJ,Coster F,Delaveau T,del Rey F,Dujon B,Eide LG,Garcia-Cantalejo JM,Goffeau A,Gomez-Peris A,Zaccaria P

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

見積依頼

Eメール

japanmarket@genscript.com

電話

03-6811-6572

FAX

03-6811-6573
050-3174-7589(受注専用)

オンラインフォーム

オンライン注文

CDC48 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

会社情報

サポート

会社情報