CDC53 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	CDC53
Entrez Gene ID	851424
Full Name	cullin CDC53
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001180191.1	NP_010150.1	cullin CDC53

Pathways from BioSystems

Homologies

Saccharomyces cerevisiae (baker's yeast)	CDC53	NP_010150.1
Eremothecium gossypii	AGOS_ADR106W	NP_984202.2
Kluyveromyces lactis	KLLA0F05467g	XP_455328.1
Oryza sativa (rice)	Os01g0369200	NP_001043049.1

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.
Jacq C, Alt-M?rbe J, Andre B, Arnold W, Bahr A, Ballesta JP, Bargues M, Baron L, Becker A, Biteau N, Bl?cker H, Blugeon C, Boskovic J, Brandt P, Br?ckner M, Buitrago MJ, Coster F, Delaveau T, del Rey F, Dujon B, Eide LG, Garcia-Cantalejo JM, Goffeau A, Gomez-Peris A, Zaccaria P
Nature387(6632 Suppl)75-8(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

This ubiquitylation and degradation pathway acts on Met30 that is dissociated from Skp1. Unexpectedly, this pathway required the cullin component Cdc53/Cul1 but was independent of the other central SCF component Skp1
Title: Skp1 Independent Function of Cdc53/Cul1 in F-box Protein Homeostasis.

A dual E3 mechanism for Rub1 ligation to Cdc53.
Title: A dual E3 mechanism for Rub1 ligation to Cdc53.

binding of substrate-specific adaptors triggers release of Cand1/Lag2, whereas subsequent neddylation of the cullin facilitates the removal and prevents re-association of Lag2/Cand1
Title: Cullin neddylation and substrate-adaptors counteract SCF inhibition by the CAND1-like protein Lag2 in Saccharomyces cerevisiae.

The binding site for Dcn1 on Cdc53 is located approximately 18 A from the site of neddylation.
Title: Dcn1 functions as a scaffold-type E3 ligase for cullin neddylation.

the SCFCdc4 dimer accommodates multiple geometries for substrate ubiquitination
Title: Suprafacial orientation of the SCFCdc4 dimer accommodates multiple geometries for substrate ubiquitination.

The following CDC53 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CDC53 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi00974	NM_001180191.1 Latest version!	Saccharomyces cerevisiae S288C cullin CDC53 (CDC53), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CDC53 ( NM_001180191.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_010150.1 Saccharomyces cerevisiae S288C cullin CDC53 (CDC53), partial mRNA.

Price & Availability↑

CloneID	OSi00974
Clone ID Related Accession (Same CDS sequence)	NM_001180191.1
Accession Version	NM_001180191.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2448bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	cullin CDC53
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001136). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGTCTGAGA CTCTGCCTAG ATCTGATGAT TTAGAGGCCA CTTGGAACTT TATAGAGCCA 
GGAATTAACC AAATACTGGG TAATGAGAAA AACCAAGCAT CCACGTCTAA GCGAGTTTAC 
AAAATTCTTT CTCCAACCAT GTATATGGAA GTCTATACCG CAATTTATAA CTACTGTGTG 
AACAAATCAC GCTCGTCTGG ACATTTTAGT ACTGACAGTA GAACTGGCCA ATCAACAATT 
TTGGTTGGCA GTGAGATTTA CGAAAAGTTA AAGAATTATT TGAAGAATTA CATTTTAAAT 
TTTAAGCAGT CTAATTCAGA GACCTTTTTG CAGTTTTACG TCAAGCGCTG GAAAAGGTTT 
ACAATAGGTG CCATTTTTTT AAACCATGCA TTCGATTATA TGAATAGATA TTGGGTTCAA 
AAGGAAAGAA GTGACGGCAA AAGGCATATT TTTGATGTAA ACACCTTGTG CTTGATGACA 
TGGAAAGAAG TCATGTTTGA TCCGAGTAAA GATGTTTTAA TAAACGAATT ATTAGACCAG 
GTAACCTTGG GAAGGGAGGG GCAAATAATT CAAAGAAGTA ATATTAGCAC TGCCATAAAG 
TCTTTAGTTG CACTAGGTAT CGATCCACAG GATTTGAAAA AGTTGAACCT AAATGTCTAC 
ATCCAAGTTT TCGAAAAGCC ATTTTTAAAG AAGACTCAGG AGTACTACAC GCAATATACA 
AACGATTATT TAGAGAAACA CTCGGTAACT GAGTATATTT TTGAAGCACA TGAAATCATC 
AAACGTGAGG AAAAAGCAAT GACAATATAT TGGGATGATC ATACAAAAAA ACCACTATCC 
ATGGCATTAA ACAAGGTCTT GATCACAGAC CATATTGAAA AGTTGGAAAA TGAGTTTGTT 
GTCCTTCTGG ATGCCAGAGA TATTGAAAAA ATTACTTCTT TGTACGCACT AATACGCAGA 
GACTTTACAT TAATCCCAAG AATGGCTTCA GTGTTTGAAA ATTATGTTAA AAAGACAGGT 
GAGAATGAAA TTTCGAGTCT ACTGGCAATG CATAAACACA ATATTATGAA AAACGAAAAC 
GCAAACCCTA AAAAACTAGC ACTAATGACA GCTCACTCCC TTTCTCCTAA AGACTATATC 
AAGAAATTAC TGGAAGTACA CGATATATTC TCTAAGATTT TTAATGAAAG TTTCCCCGAC 
GACATACCTT TAGCTAAAGC TCTAGATAAT GCGTGTGGTG CTTTCATCAA CATCAACGAG 
TTCGCATTAC CTGCTGGATC TCCAAAAAGT GCCACCTCGA AGACTTCCGA GATGCTAGCT 
AAGTACAGTG ATATACTATT AAAGAAGGCC ACCAAACCTG AAGTGGCAAG TGACATGTCA 
GATGAAGATA TTATAACAAT ATTCAAATAT TTGACCGACA AAGATGCGTT CGAAACTCAT 
TATAGAAGAC TTTTCGCCAA GCGTTTAATT CATGGCACTT CAACATCAGC AGAAGACGAA 
GAAAATATTA TTCAAAGGCT GCAAGCGGCA AATAGTATGG AATATACAGG CAAGATAACT 
AAAATGTTTC AAGATATTAG ACTTTCCAAG ATCTTGGAAG ACGATTTTGC TGTCGCCCTG 
AAGAATGAAC CAGATTACTC TAAAGCAAAA TATCCAGATC TACAACCATT TGTATTGGCA 
GAAAATATGT GGCCATTTTC ATACCAAGAA GTTGAATTTA AGCTACCCAA GGAATTGGTA 
CCATCTCACG AAAAATTGAA GGAGTCATAC AGCCAAAAGC ATAATGGTAG AATATTGAAG 
TGGCTATGGC CATTATGTCG TGGCGAATTG AAGGCAGATA TTGGAAAACC CGGCAGGATG 
CCGTTCAACT TTACGGTAAC ACTGTTTCAA ATGGCAATTT TGCTACTATA TAACGATGCT 
GATGTATTAA CCTTAGAAAA TATTCAAGAG GGCACGAGCT TAACCATTCA GCACATCGCA 
GCGGCAATGG TACCATTCAT CAAATTCAAA CTGATTCAAC AGGTCCCACC AGGGCTAGAT 
GCTCTCGTGA AACCTGAAAC ACAATTCAAG CTATCACGTC CTTATAAGGC GCTTAAGACC 
AATATTAATT TCGCTAGCGG AGTCAAAAAC GACATTCTGC AATCGCTATC CGGTGGAGGC 
CACGATAATC ACGGTAATAA GTTAGGAAAC AAACGCTTGA CGGAAGACGA AAGAATAGAA 
AAAGAATTGA ATACAGAAAG GCAGATTTTC TTGGAAGCTT GTATTGTGAG AATAATGAAA 
GCGAAAAGAA ATCTGCCACA TACAACATTG GTCAACGAAT GCATTGCGCA ATCACACCAA 
AGGTTCAATG CAAAGGTTTC TATGGTCAAG AGAGCCATCG ATAGCTTAAT ACAAAAGGGA 
TACCTACAGA GGGGAGACGA TGGTGAATCG TATGCTTACC TTGCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_010150.1
CDS	1..2448
Translation	MSETLPRSDDLEATWNFIEPGINQILGNEKNQASTSKRVYKILSPTMYMEVYTAIYNYCVNKSRSSGHFSTDSRTGQSTILVGSEIYEKLKNYLKNYILNFKQSNSETFLQFYVKRWKRFTIGAIFLNHAFDYMNRYWVQKERSDGKRHIFDVNTLCLMTWKEVMFDPSKDVLINELLDQVTLGREGQIIQRSNISTAIKSLVALGIDPQDLKKLNLNVYIQVFEKPFLKKTQEYYTQYTNDYLEKHSVTEYIFEAHEIIKREEKAMTIYWDDHTKKPLSMALNKVLITDHIEKLENEFVVLLDARDIEKITSLYALIRRDFTLIPRMASVFENYVKKTGENEISSLLAMHKHNIMKNENANPKKLALMTAHSLSPKDYIKKLLEVHDIFSKIFNESFPDDIPLAKALDNACGAFININEFALPAGSPKSATSKTSEMLAKYSDILLKKATKPEVASDMSDEDIITIFKYLTDKDAFETHYRRLFAKRLIHGTSTSAEDEENIIQRLQAANSMEYTGKITKMFQDIRLSKILEDDFAVALKNEPDYSKAKYPDLQPFVLAENMWPFSYQEVEFKLPKELVPSHEKLKESYSQKHNGRILKWLWPLCRGELKADIGKPGRMPFNFTVTLFQMAILLLYNDADVLTLENIQEGTSLTIQHIAAAMVPFIKFKLIQQVPPGLDALVKPETQFKLSRPYKALKTNINFASGVKNDILQSLSGGGHDNHGNKLGNKRLTEDERIEKELNTERQIFLEACIVRIMKAKRNLPHTTLVNECIAQSHQRFNAKVSMVKRAIDSLIQKGYLQRGDDGESYAYLA

Target ORF information:

RefSeq Version	NM_001180191.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C cullin CDC53 (CDC53), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001180191.1

ORF Insert Sequence:

ATGTCTGAGA CTCTGCCTAG ATCTGATGAT TTAGAGGCCA CTTGGAACTT TATAGAGCCA 
GGAATTAACC AAATACTGGG TAATGAGAAA AACCAAGCAT CCACGTCTAA GCGAGTTTAC 
AAAATTCTTT CTCCAACCAT GTATATGGAA GTCTATACCG CAATTTATAA CTACTGTGTG 
AACAAATCAC GCTCGTCTGG ACATTTTAGT ACTGACAGTA GAACTGGCCA ATCAACAATT 
TTGGTTGGCA GTGAGATTTA CGAAAAGTTA AAGAATTATT TGAAGAATTA CATTTTAAAT 
TTTAAGCAGT CTAATTCAGA GACCTTTTTG CAGTTTTACG TCAAGCGCTG GAAAAGGTTT 
ACAATAGGTG CCATTTTTTT AAACCATGCA TTCGATTATA TGAATAGATA TTGGGTTCAA 
AAGGAAAGAA GTGACGGCAA AAGGCATATT TTTGATGTAA ACACCTTGTG CTTGATGACA 
TGGAAAGAAG TCATGTTTGA TCCGAGTAAA GATGTTTTAA TAAACGAATT ATTAGACCAG 
GTAACCTTGG GAAGGGAGGG GCAAATAATT CAAAGAAGTA ATATTAGCAC TGCCATAAAG 
TCTTTAGTTG CACTAGGTAT CGATCCACAG GATTTGAAAA AGTTGAACCT AAATGTCTAC 
ATCCAAGTTT TCGAAAAGCC ATTTTTAAAG AAGACTCAGG AGTACTACAC GCAATATACA 
AACGATTATT TAGAGAAACA CTCGGTAACT GAGTATATTT TTGAAGCACA TGAAATCATC 
AAACGTGAGG AAAAAGCAAT GACAATATAT TGGGATGATC ATACAAAAAA ACCACTATCC 
ATGGCATTAA ACAAGGTCTT GATCACAGAC CATATTGAAA AGTTGGAAAA TGAGTTTGTT 
GTCCTTCTGG ATGCCAGAGA TATTGAAAAA ATTACTTCTT TGTACGCACT AATACGCAGA 
GACTTTACAT TAATCCCAAG AATGGCTTCA GTGTTTGAAA ATTATGTTAA AAAGACAGGT 
GAGAATGAAA TTTCGAGTCT ACTGGCAATG CATAAACACA ATATTATGAA AAACGAAAAC 
GCAAACCCTA AAAAACTAGC ACTAATGACA GCTCACTCCC TTTCTCCTAA AGACTATATC 
AAGAAATTAC TGGAAGTACA CGATATATTC TCTAAGATTT TTAATGAAAG TTTCCCCGAC 
GACATACCTT TAGCTAAAGC TCTAGATAAT GCGTGTGGTG CTTTCATCAA CATCAACGAG 
TTCGCATTAC CTGCTGGATC TCCAAAAAGT GCCACCTCGA AGACTTCCGA GATGCTAGCT 
AAGTACAGTG ATATACTATT AAAGAAGGCC ACCAAACCTG AAGTGGCAAG TGACATGTCA 
GATGAAGATA TTATAACAAT ATTCAAATAT TTGACCGACA AAGATGCGTT CGAAACTCAT 
TATAGAAGAC TTTTCGCCAA GCGTTTAATT CATGGCACTT CAACATCAGC AGAAGACGAA 
GAAAATATTA TTCAAAGGCT GCAAGCGGCA AATAGTATGG AATATACAGG CAAGATAACT 
AAAATGTTTC AAGATATTAG ACTTTCCAAG ATCTTGGAAG ACGATTTTGC TGTCGCCCTG 
AAGAATGAAC CAGATTACTC TAAAGCAAAA TATCCAGATC TACAACCATT TGTATTGGCA 
GAAAATATGT GGCCATTTTC ATACCAAGAA GTTGAATTTA AGCTACCCAA GGAATTGGTA 
CCATCTCACG AAAAATTGAA GGAGTCATAC AGCCAAAAGC ATAATGGTAG AATATTGAAG 
TGGCTATGGC CATTATGTCG TGGCGAATTG AAGGCAGATA TTGGAAAACC CGGCAGGATG 
CCGTTCAACT TTACGGTAAC ACTGTTTCAA ATGGCAATTT TGCTACTATA TAACGATGCT 
GATGTATTAA CCTTAGAAAA TATTCAAGAG GGCACGAGCT TAACCATTCA GCACATCGCA 
GCGGCAATGG TACCATTCAT CAAATTCAAA CTGATTCAAC AGGTCCCACC AGGGCTAGAT 
GCTCTCGTGA AACCTGAAAC ACAATTCAAG CTATCACGTC CTTATAAGGC GCTTAAGACC 
AATATTAATT TCGCTAGCGG AGTCAAAAAC GACATTCTGC AATCGCTATC CGGTGGAGGC 
CACGATAATC ACGGTAATAA GTTAGGAAAC AAACGCTTGA CGGAAGACGA AAGAATAGAA 
AAAGAATTGA ATACAGAAAG GCAGATTTTC TTGGAAGCTT GTATTGTGAG AATAATGAAA 
GCGAAAAGAA ATCTGCCACA TACAACATTG GTCAACGAAT GCATTGCGCA ATCACACCAA 
AGGTTCAATG CAAAGGTTTC TATGGTCAAG AGAGCCATCG ATAGCTTAAT ACAAAAGGGA 
TACCTACAGA GGGGAGACGA TGGTGAATCG TATGCTTACC TTGCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.
Nature387(6632 Suppl)75-8(1997 May)
Jacq C,Alt-M?rbe J,Andre B,Arnold W,Bahr A,Ballesta JP,Bargues M,Baron L,Becker A,Biteau N,Bl?cker H,Blugeon C,Boskovic J,Brandt P,Br?ckner M,Buitrago MJ,Coster F,Delaveau T,del Rey F,Dujon B,Eide LG,Garcia-Cantalejo JM,Goffeau A,Gomez-Peris A,Zaccaria P

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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CDC53 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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