RPO21 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	RPO21
Entrez Gene ID	851415
Full Name	DNA-directed RNA polymerase II core subunit RPO21
Synonyms	RPB1,RPB220,SUA8
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001180200.1	NP_010141.1	DNA-directed RNA polymerase II core subunit RPO21

Pathways from BioSystems

Homologies

Schizosaccharomyces pombe (fission yeast)	rpb1	NP_595673.1
Homo sapiens (human)	POLR2A	NP_000928.1
Pan troglodytes (chimpanzee)	POLR2A	XP_511300.3
Neurospora crassa	NCU02103	XP_964097.1
Oryza sativa (rice)	Os05g0151000	NP_001054670.2
Canis lupus familiaris (dog)	POLR2A	XP_857844.2
Bos taurus (cattle)	POLR2A	NP_001193242.1
Rattus norvegicus (Norway rat)	Polr2a	XP_343923.3
Caenorhabditis elegans (roundworm)	ama-1	NP_500523.4
Xenopus tropicalis (tropical clawed frog)	polr2a	XP_002943327.2
	POLR2A	XP_001118060.2
Mus musculus (house mouse)	Polr2a	NP_033115.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007810	XP_317690.3
Saccharomyces cerevisiae (baker's yeast)	RPO21	NP_010141.1
Magnaporthe oryzae (rice blast fungus)	MGG_04652	XP_003713642.1
Arabidopsis thaliana (thale cress)	NRPB1	NP_195305.2
Danio rerio (zebrafish)	polr2a	XP_005156282.1
Drosophila melanogaster (fruit fly)	RpII215	NP_511124.1
Kluyveromyces lactis	KLLA0F05071g	XP_455310.1
Eremothecium gossypii	AGOS_ADR086C	NP_984182.2

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.
Jacq C, Alt-M?rbe J, Andre B, Arnold W, Bahr A, Ballesta JP, Bargues M, Baron L, Becker A, Biteau N, Bl?cker H, Blugeon C, Boskovic J, Brandt P, Br?ckner M, Buitrago MJ, Coster F, Delaveau T, del Rey F, Dujon B, Eide LG, Garcia-Cantalejo JM, Goffeau A, Gomez-Peris A, Zaccaria P
Nature387(6632 Suppl)75-8(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Promoter and template-specific effects on severity of gene expression defects have been identified for both fast and slow Pol II mutants.
Title: Wide-ranging and unexpected consequences of altered Pol II catalytic activity in vivo.

Studies indicate that the covalent, flexible linker ensured that transcription factor TFIIB (TFIIB) was recruited stoichiometrically near its binding site on the RNA polymerase (Pol) II (Pol II) surface.
Title: Structure determination of transient transcription complexes.

RPO21 interaction with LYS2 and Spt elongation factors
Title: Relationships Between RNA Polymerase II Activity and Spt Elongation Factors to Spt- Phenotype and Growth in Saccharomyces cerevisiae.

RPB1 mutants in the foot region of RNA polymerase II affect the assembly of the complex by altering the correct association of both the Rpb6 and the Rpb4/7 dimer
Title: Rpb1 foot mutations demonstrate a major role of Rpb4 in mRNA stability during stress situations in yeast.

Results indicate that multiple mechanistic features contribute to 5'-3' exoribonuclease Rat1-mediated termination of RNA polymerase II (RNAPII).
Title: Unraveling the mechanistic features of RNA polymerase II termination by the 5'-3' exoribonuclease Rat1.

The following RPO21 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RPO21 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi00965	NM_001180200.1 Latest version!	Saccharomyces cerevisiae S288C DNA-directed RNA polymerase II core subunit RPO21 (RPO21), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RPO21 ( NM_001180200.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_010141.1 Saccharomyces cerevisiae S288C DNA-directed RNA polymerase II core subunit RPO21 (RPO21), partial mRNA.

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CloneID	OSi00965
Clone ID Related Accession (Same CDS sequence)	NM_001180200.1
Accession Version	NM_001180200.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5202bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	DNA-directed RNA polymerase II core subunit RPO21
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001136). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGGTAGGAC AACAGTATTC TAGTGCTCCA CTCCGTACAG TAAAAGAGGT CCAATTCGGT 
CTTTTCTCAC CTGAAGAAGT TAGAGCAATC AGTGTGGCCA AAATTAGATT TCCAGAGACA 
ATGGATGAAA CCCAGACGAG AGCGAAAATT GGTGGTCTAA ACGACCCTAG GTTAGGCTCT 
ATTGATCGTA ATCTGAAGTG TCAAACTTGT CAAGAGGGTA TGAACGAATG TCCTGGTCAT 
TTTGGTCACA TAGATTTAGC AAAACCTGTA TTTCATGTTG GTTTTATTGC CAAAATTAAG 
AAAGTATGTG AGTGTGTCTG TATGCACTGT GGTAAGCTAT TACTGGATGA ACATAATGAA 
TTAATGAGAC AAGCTCTAGC AATCAAAGAC AGTAAAAAAA GGTTTGCTGC AATTTGGACT 
TTATGTAAAA CAAAAATGGT CTGCGAAACA GATGTCCCTT CTGAAGATGA TCCTACTCAG 
CTCGTATCAA GGGGAGGTTG TGGTAATACA CAGCCTACAA TTCGTAAGGA TGGGTTGAAA 
TTAGTTGGTA GTTGGAAAAA AGATAGAGCC ACGGGGGATG CGGATGAACC AGAACTAAGA 
GTTTTAAGTA CGGAGGAAAT CTTGAATATT TTTAAGCATA TCTCAGTAAA AGACTTCACT 
AGTTTGGGTT TCAACGAAGT TTTTTCTCGT CCAGAATGGA TGATTTTAAC ATGCCTTCCT 
GTCCCACCAC CACCGGTGCG TCCATCCATT TCCTTCAATG AATCTCAAAG AGGTGAGGAT 
GATTTAACCT TTAAACTTGC TGATATTTTA AAAGCTAATA TTAGTTTGGA AACACTAGAG 
CATAACGGTG CTCCACATCA TGCTATTGAA GAAGCAGAGA GTTTATTACA ATTTCATGTT 
GCCACTTATA TGGATAATGA TATTGCTGGT CAACCACAAG CTCTTCAAAA GTCCGGCCGT 
CCCGTTAAAT CTATTCGTGC TCGTTTGAAG GGTAAAGAGG GTCGTATCAG AGGTAATTTA 
ATGGGTAAGC GTGTGGATTT TTCGGCAAGA ACTGTTATTT CTGGTGATCC TAATTTGGAA 
TTAGACCAAG TCGGTGTTCC AAAATCTATT GCCAAGACTT TAACATACCC AGAAGTGGTC 
ACACCATATA ACATAGATCG TCTGACGCAA CTTGTTAGGA ATGGACCAAA TGAGCACCCC 
GGTGCCAAAT ACGTCATTCG TGATAGCGGA GACCGTATAG ATTTAAGATA CAGTAAAAGG 
GCAGGTGATA TTCAATTACA GTATGGGTGG AAAGTTGAAC GTCATATTAT GGACAATGAT 
CCAGTTTTAT TCAACCGTCA ACCTTCGTTG CACAAAATGT CCATGATGGC CCACAGAGTA 
AAAGTTATTC CATATTCTAC ATTTAGATTG AATTTGTCCG TTACATCTCC ATACAATGCC 
GATTTCGACG GTGACGAAAT GAATCTTCAC GTTCCTCAGT CTGAGGAAAC AAGGGCGGAA 
CTTTCTCAAT TATGTGCTGT TCCTCTGCAA ATTGTTTCAC CACAATCTAA CAAACCTTGT 
ATGGGTATTG TTCAAGATAC TTTGTGTGGT ATTCGTAAAC TGACATTAAG AGATACATTT 
ATAGAACTTG ATCAAGTTTT GAATATGCTT TATTGGGTTC CAGATTGGGA TGGTGTTATT 
CCGACACCTG CAATTATCAA GCCCAAACCT TTGTGGTCCG GTAAACAAAT CTTGTCTGTG 
GCTATCCCAA ACGGTATTCA TTTACAACGT TTTGATGAGG GCACTACTCT GCTTTCTCCA 
AAGGATAATG GTATGCTTAT TATTGACGGT CAAATCATTT TTGGTGTAGT AGAGAAAAAA 
ACCGTTGGTT CCTCCAATGG TGGTTTAATT CATGTTGTTA CGAGAGAAAA GGGACCTCAA 
GTTTGTGCTA AGTTGTTTGG TAACATACAG AAAGTTGTTA ACTTTTGGTT ACTACATAAT 
GGGTTTTCAA CAGGTATTGG TGATACCATT GCGGACGGCC CAACAATGAG GGAAATTACA 
GAGACAATTG CAGAGGCTAA AAAGAAAGTT TTGGATGTTA CGAAAGAAGC CCAGGCAAAC 
TTATTGACTG CTAAACATGG TATGACTCTC CGTGAGTCTT TTGAGGATAA CGTTGTTCGG 
TTCCTAAATG AAGCAAGAGA TAAGGCAGGT CGTTTAGCTG AAGTCAATTT GAAAGATTTG 
AACAATGTGA AACAAATGGT TATGGCAGGT TCCAAGGGTT CATTTATTAA TATCGCGCAA 
ATGTCAGCTT GTGTAGGACA GCAATCTGTT GAAGGTAAAC GTATTGCTTT TGGGTTCGTT 
GATCGTACCT TACCTCATTT CTCTAAAGAT GATTACTCCC CAGAGTCTAA AGGTTTTGTT 
GAGAACTCAT ATTTGAGAGG TTTGACCCCA CAAGAATTTT TTTTCCATGC AATGGGTGGT 
CGTGAAGGTC TTATCGATAC CGCCGTCAAA ACAGCCGAAA CAGGTTATAT TCAACGTCGT 
TTAGTGAAAG CTCTAGAAGA TATCATGGTT CATTACGATA ACACCACAAG AAACTCATTG 
GGTAACGTTA TTCAGTTTAT TTATGGTGAA GATGGTATGG ATGCTGCGCA TATTGAAAAG 
CAATCGCTAG ATACTATTGG TGGCTCCGAT GCAGCTTTTG AAAAGAGATA CAGAGTTGAT 
TTATTGAATA CAGACCATAC CCTTGATCCC TCACTATTGG AATCCGGATC TGAGATACTT 
GGCGATTTGA AACTTCAAGT TCTCCTGGAT GAAGAATACA AACAATTAGT GAAAGATCGT 
AAATTTTTGA GGGAAGTTTT TGTTGATGGT GAAGCAAACT GGCCATTACC AGTCAACATA 
AGACGTATTA TTCAAAATGC TCAACAAACT TTCCACATAG ATCATACGAA ACCATCTGAT 
TTAACAATCA AAGACATCGT TCTTGGTGTA AAGGATTTGC AAGAAAACTT ATTAGTGTTG 
CGTGGTAAGA ATGAAATTAT ACAAAATGCC CAGCGAGATG CAGTTACATT GTTCTGCTGT 
TTATTACGTT CCCGTTTGGC CACACGTAGA GTTCTACAAG AGTACAGACT AACAAAACAG 
GCATTCGATT GGGTATTAAG TAATATCGAG GCACAATTCC TCCGTTCTGT TGTTCACCCT 
GGTGAAATGG TTGGTGTTCT AGCAGCCCAA TCCATTGGTG AACCAGCCAC ACAAATGACC 
CTTAACACCT TCCATTTTGC TGGTGTTGCT TCCAAAAAAG TTACTTCTGG TGTCCCCCGT 
TTAAAGGAAA TTTTGAATGT GGCCAAAAAC ATGAAAACCC CTTCCTTGAC TGTATACTTA 
GAGCCTGGTC ATGCTGCCGA TCAAGAACAA GCGAAGTTGA TCAGATCTGC TATCGAGCAT 
ACCACTTTAA AGAGTGTCAC TATTGCTTCA GAAATTTACT ATGATCCTGA TCCACGTTCC 
ACAGTTATTC CAGAAGATGA AGAAATTATC CAACTTCATT TCTCATTATT GGATGAAGAG 
GCTGAACAAT CTTTTGACCA ACAATCACCT TGGTTATTAC GTCTGGAACT GGATCGTGCA 
GCAATGAATG ATAAAGACTT AACAATGGGT CAGGTTGGTG AAAGAATCAA GCAAACATTC 
AAAAATGATT TGTTTGTTAT CTGGTCTGAA GACAACGATG AGAAGTTGAT CATCCGTTGT 
CGTGTTGTTC GTCCAAAGTC ACTAGATGCT GAGACTGAAG CAGAAGAAGA TCATATGTTG 
AAGAAAATTG AGAACACAAT GTTAGAGAAT ATTACATTAC GTGGTGTAGA GAACATCGAG 
CGTGTTGTCA TGATGAAATA TGACCGTAAA GTACCAAGTC CAACTGGTGA ATACGTTAAG 
GAACCTGAAT GGGTGTTGGA AACAGATGGT GTTAACTTAT CTGAAGTTAT GACTGTTCCT 
GGTATCGACC CAACCAGAAT CTATACCAAC TCCTTCATTG ATATAATGGA AGTTCTAGGT 
ATTGAAGCTG GTCGTGCAGC CTTGTATAAA GAAGTTTACA ATGTTATTGC TTCTGATGGT 
TCGTATGTTA ACTACCGTCA TATGGCTTTG TTAGTCGATG TTATGACAAC CCAAGGTGGC 
TTAACTTCTG TTACTCGTCA TGGTTTCAAC AGATCAAATA CAGGTGCCTT AATGAGATGT 
TCATTTGAAG AAACTGTCGA AATTTTGTTT GAAGCTGGTG CTTCAGCCGA ATTAGATGAT 
TGTCGTGGTG TTTCGGAAAA TGTCATTCTT GGTCAAATGG CTCCAATCGG TACCGGTGCA 
TTTGATGTGA TGATCGATGA GGAGTCACTG GTAAAATACA TGCCAGAACA AAAAATAACT 
GAGATTGAAG ACGGACAAGA TGGTGGCGTC ACACCATACA GTAACGAAAG TGGTTTGGTC 
AATGCAGATC TTGACGTTAA AGATGAGCTA ATGTTTTCAC CTCTGGTTGA TTCGGGTTCA 
AATGACGCTA TGGCTGGAGG ATTTACAGCG TACGGTGGTG CTGATTATGG TGAAGCCACG 
TCTCCATTTG GTGCTTATGG TGAAGCACCT ACATCTCCCG GATTTGGAGT CTCCTCACCA 
GGCTTTTCTC CAACTTCCCC AACATACTCT CCTACCTCTC CAGCGTACTC ACCAACATCA 
CCATCGTACT CACCAACATC ACCATCGTAC TCGCCAACAT CACCATCGTA CTCACCTACA 
TCACCATCGT ATTCACCAAC GTCACCATCA TATTCGCCAA CGTCACCATC ATATTCGCCA 
ACGTCGCCAT CGTATTCTCC AACGTCACCA TCGTATTCGC CAACGTCGCC TTCCTACTCT 
CCCACGTCGC CAAGCTACAG CCCTACGTCT CCTTCTTATT CTCCTACATC TCCATCATAC 
TCTCCTACGT CACCAAGTTA CAGCCCAACG TCACCAAGTT ACAGCCCAAC GTCTCCAGCC 
TATTCCCCAA CATCACCAAG TTATAGTCCT ACATCGCCTT CATACTCTCC AACATCACCA 
TCCTATTCCC CAACATCACC TTCTTACTCT CCCACCTCTC CAAACTATAG CCCTACTTCA 
CCTTCTTACT CCCCAACATC TCCAGGCTAC AGCCCAGGAT CTCCTGCATA TTCTCCAAAG 
CAAGACGAAC AAAAGCATAA TGAAAATGAA AATTCCAGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_010141.1
CDS	1..5202
Translation	MVGQQYSSAPLRTVKEVQFGLFSPEEVRAISVAKIRFPETMDETQTRAKIGGLNDPRLGSIDRNLKCQTCQEGMNECPGHFGHIDLAKPVFHVGFIAKIKKVCECVCMHCGKLLLDEHNELMRQALAIKDSKKRFAAIWTLCKTKMVCETDVPSEDDPTQLVSRGGCGNTQPTIRKDGLKLVGSWKKDRATGDADEPELRVLSTEEILNIFKHISVKDFTSLGFNEVFSRPEWMILTCLPVPPPPVRPSISFNESQRGEDDLTFKLADILKANISLETLEHNGAPHHAIEEAESLLQFHVATYMDNDIAGQPQALQKSGRPVKSIRARLKGKEGRIRGNLMGKRVDFSARTVISGDPNLELDQVGVPKSIAKTLTYPEVVTPYNIDRLTQLVRNGPNEHPGAKYVIRDSGDRIDLRYSKRAGDIQLQYGWKVERHIMDNDPVLFNRQPSLHKMSMMAHRVKVIPYSTFRLNLSVTSPYNADFDGDEMNLHVPQSEETRAELSQLCAVPLQIVSPQSNKPCMGIVQDTLCGIRKLTLRDTFIELDQVLNMLYWVPDWDGVIPTPAIIKPKPLWSGKQILSVAIPNGIHLQRFDEGTTLLSPKDNGMLIIDGQIIFGVVEKKTVGSSNGGLIHVVTREKGPQVCAKLFGNIQKVVNFWLLHNGFSTGIGDTIADGPTMREITETIAEAKKKVLDVTKEAQANLLTAKHGMTLRESFEDNVVRFLNEARDKAGRLAEVNLKDLNNVKQMVMAGSKGSFINIAQMSACVGQQSVEGKRIAFGFVDRTLPHFSKDDYSPESKGFVENSYLRGLTPQEFFFHAMGGREGLIDTAVKTAETGYIQRRLVKALEDIMVHYDNTTRNSLGNVIQFIYGEDGMDAAHIEKQSLDTIGGSDAAFEKRYRVDLLNTDHTLDPSLLESGSEILGDLKLQVLLDEEYKQLVKDRKFLREVFVDGEANWPLPVNIRRIIQNAQQTFHIDHTKPSDLTIKDIVLGVKDLQENLLVLRGKNEIIQNAQRDAVTLFCCLLRSRLATRRVLQEYRLTKQAFDWVLSNIEAQFLRSVVHPGEMVGVLAAQSIGEPATQMTLNTFHFAGVASKKVTSGVPRLKEILNVAKNMKTPSLTVYLEPGHAADQEQAKLIRSAIEHTTLKSVTIASEIYYDPDPRSTVIPEDEEIIQLHFSLLDEEAEQSFDQQSPWLLRLELDRAAMNDKDLTMGQVGERIKQTFKNDLFVIWSEDNDEKLIIRCRVVRPKSLDAETEAEEDHMLKKIENTMLENITLRGVENIERVVMMKYDRKVPSPTGEYVKEPEWVLETDGVNLSEVMTVPGIDPTRIYTNSFIDIMEVLGIEAGRAALYKEVYNVIASDGSYVNYRHMALLVDVMTTQGGLTSVTRHGFNRSNTGALMRCSFEETVEILFEAGASAELDDCRGVSENVILGQMAPIGTGAFDVMIDEESLVKYMPEQKITEIEDGQDGGVTPYSNESGLVNADLDVKDELMFSPLVDSGSNDAMAGGFTAYGGADYGEATSPFGAYGEAPTSPGFGVSSPGFSPTSPTYSPTSPAYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPAYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPNYSPTSPSYSPTSPGYSPGSPAYSPKQDEQKHNENENSR

Target ORF information:

RefSeq Version	NM_001180200.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C DNA-directed RNA polymerase II core subunit RPO21 (RPO21), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001180200.1

ORF Insert Sequence:

ATGGTAGGAC AACAGTATTC TAGTGCTCCA CTCCGTACAG TAAAAGAGGT CCAATTCGGT 
CTTTTCTCAC CTGAAGAAGT TAGAGCAATC AGTGTGGCCA AAATTAGATT TCCAGAGACA 
ATGGATGAAA CCCAGACGAG AGCGAAAATT GGTGGTCTAA ACGACCCTAG GTTAGGCTCT 
ATTGATCGTA ATCTGAAGTG TCAAACTTGT CAAGAGGGTA TGAACGAATG TCCTGGTCAT 
TTTGGTCACA TAGATTTAGC AAAACCTGTA TTTCATGTTG GTTTTATTGC CAAAATTAAG 
AAAGTATGTG AGTGTGTCTG TATGCACTGT GGTAAGCTAT TACTGGATGA ACATAATGAA 
TTAATGAGAC AAGCTCTAGC AATCAAAGAC AGTAAAAAAA GGTTTGCTGC AATTTGGACT 
TTATGTAAAA CAAAAATGGT CTGCGAAACA GATGTCCCTT CTGAAGATGA TCCTACTCAG 
CTCGTATCAA GGGGAGGTTG TGGTAATACA CAGCCTACAA TTCGTAAGGA TGGGTTGAAA 
TTAGTTGGTA GTTGGAAAAA AGATAGAGCC ACGGGGGATG CGGATGAACC AGAACTAAGA 
GTTTTAAGTA CGGAGGAAAT CTTGAATATT TTTAAGCATA TCTCAGTAAA AGACTTCACT 
AGTTTGGGTT TCAACGAAGT TTTTTCTCGT CCAGAATGGA TGATTTTAAC ATGCCTTCCT 
GTCCCACCAC CACCGGTGCG TCCATCCATT TCCTTCAATG AATCTCAAAG AGGTGAGGAT 
GATTTAACCT TTAAACTTGC TGATATTTTA AAAGCTAATA TTAGTTTGGA AACACTAGAG 
CATAACGGTG CTCCACATCA TGCTATTGAA GAAGCAGAGA GTTTATTACA ATTTCATGTT 
GCCACTTATA TGGATAATGA TATTGCTGGT CAACCACAAG CTCTTCAAAA GTCCGGCCGT 
CCCGTTAAAT CTATTCGTGC TCGTTTGAAG GGTAAAGAGG GTCGTATCAG AGGTAATTTA 
ATGGGTAAGC GTGTGGATTT TTCGGCAAGA ACTGTTATTT CTGGTGATCC TAATTTGGAA 
TTAGACCAAG TCGGTGTTCC AAAATCTATT GCCAAGACTT TAACATACCC AGAAGTGGTC 
ACACCATATA ACATAGATCG TCTGACGCAA CTTGTTAGGA ATGGACCAAA TGAGCACCCC 
GGTGCCAAAT ACGTCATTCG TGATAGCGGA GACCGTATAG ATTTAAGATA CAGTAAAAGG 
GCAGGTGATA TTCAATTACA GTATGGGTGG AAAGTTGAAC GTCATATTAT GGACAATGAT 
CCAGTTTTAT TCAACCGTCA ACCTTCGTTG CACAAAATGT CCATGATGGC CCACAGAGTA 
AAAGTTATTC CATATTCTAC ATTTAGATTG AATTTGTCCG TTACATCTCC ATACAATGCC 
GATTTCGACG GTGACGAAAT GAATCTTCAC GTTCCTCAGT CTGAGGAAAC AAGGGCGGAA 
CTTTCTCAAT TATGTGCTGT TCCTCTGCAA ATTGTTTCAC CACAATCTAA CAAACCTTGT 
ATGGGTATTG TTCAAGATAC TTTGTGTGGT ATTCGTAAAC TGACATTAAG AGATACATTT 
ATAGAACTTG ATCAAGTTTT GAATATGCTT TATTGGGTTC CAGATTGGGA TGGTGTTATT 
CCGACACCTG CAATTATCAA GCCCAAACCT TTGTGGTCCG GTAAACAAAT CTTGTCTGTG 
GCTATCCCAA ACGGTATTCA TTTACAACGT TTTGATGAGG GCACTACTCT GCTTTCTCCA 
AAGGATAATG GTATGCTTAT TATTGACGGT CAAATCATTT TTGGTGTAGT AGAGAAAAAA 
ACCGTTGGTT CCTCCAATGG TGGTTTAATT CATGTTGTTA CGAGAGAAAA GGGACCTCAA 
GTTTGTGCTA AGTTGTTTGG TAACATACAG AAAGTTGTTA ACTTTTGGTT ACTACATAAT 
GGGTTTTCAA CAGGTATTGG TGATACCATT GCGGACGGCC CAACAATGAG GGAAATTACA 
GAGACAATTG CAGAGGCTAA AAAGAAAGTT TTGGATGTTA CGAAAGAAGC CCAGGCAAAC 
TTATTGACTG CTAAACATGG TATGACTCTC CGTGAGTCTT TTGAGGATAA CGTTGTTCGG 
TTCCTAAATG AAGCAAGAGA TAAGGCAGGT CGTTTAGCTG AAGTCAATTT GAAAGATTTG 
AACAATGTGA AACAAATGGT TATGGCAGGT TCCAAGGGTT CATTTATTAA TATCGCGCAA 
ATGTCAGCTT GTGTAGGACA GCAATCTGTT GAAGGTAAAC GTATTGCTTT TGGGTTCGTT 
GATCGTACCT TACCTCATTT CTCTAAAGAT GATTACTCCC CAGAGTCTAA AGGTTTTGTT 
GAGAACTCAT ATTTGAGAGG TTTGACCCCA CAAGAATTTT TTTTCCATGC AATGGGTGGT 
CGTGAAGGTC TTATCGATAC CGCCGTCAAA ACAGCCGAAA CAGGTTATAT TCAACGTCGT 
TTAGTGAAAG CTCTAGAAGA TATCATGGTT CATTACGATA ACACCACAAG AAACTCATTG 
GGTAACGTTA TTCAGTTTAT TTATGGTGAA GATGGTATGG ATGCTGCGCA TATTGAAAAG 
CAATCGCTAG ATACTATTGG TGGCTCCGAT GCAGCTTTTG AAAAGAGATA CAGAGTTGAT 
TTATTGAATA CAGACCATAC CCTTGATCCC TCACTATTGG AATCCGGATC TGAGATACTT 
GGCGATTTGA AACTTCAAGT TCTCCTGGAT GAAGAATACA AACAATTAGT GAAAGATCGT 
AAATTTTTGA GGGAAGTTTT TGTTGATGGT GAAGCAAACT GGCCATTACC AGTCAACATA 
AGACGTATTA TTCAAAATGC TCAACAAACT TTCCACATAG ATCATACGAA ACCATCTGAT 
TTAACAATCA AAGACATCGT TCTTGGTGTA AAGGATTTGC AAGAAAACTT ATTAGTGTTG 
CGTGGTAAGA ATGAAATTAT ACAAAATGCC CAGCGAGATG CAGTTACATT GTTCTGCTGT 
TTATTACGTT CCCGTTTGGC CACACGTAGA GTTCTACAAG AGTACAGACT AACAAAACAG 
GCATTCGATT GGGTATTAAG TAATATCGAG GCACAATTCC TCCGTTCTGT TGTTCACCCT 
GGTGAAATGG TTGGTGTTCT AGCAGCCCAA TCCATTGGTG AACCAGCCAC ACAAATGACC 
CTTAACACCT TCCATTTTGC TGGTGTTGCT TCCAAAAAAG TTACTTCTGG TGTCCCCCGT 
TTAAAGGAAA TTTTGAATGT GGCCAAAAAC ATGAAAACCC CTTCCTTGAC TGTATACTTA 
GAGCCTGGTC ATGCTGCCGA TCAAGAACAA GCGAAGTTGA TCAGATCTGC TATCGAGCAT 
ACCACTTTAA AGAGTGTCAC TATTGCTTCA GAAATTTACT ATGATCCTGA TCCACGTTCC 
ACAGTTATTC CAGAAGATGA AGAAATTATC CAACTTCATT TCTCATTATT GGATGAAGAG 
GCTGAACAAT CTTTTGACCA ACAATCACCT TGGTTATTAC GTCTGGAACT GGATCGTGCA 
GCAATGAATG ATAAAGACTT AACAATGGGT CAGGTTGGTG AAAGAATCAA GCAAACATTC 
AAAAATGATT TGTTTGTTAT CTGGTCTGAA GACAACGATG AGAAGTTGAT CATCCGTTGT 
CGTGTTGTTC GTCCAAAGTC ACTAGATGCT GAGACTGAAG CAGAAGAAGA TCATATGTTG 
AAGAAAATTG AGAACACAAT GTTAGAGAAT ATTACATTAC GTGGTGTAGA GAACATCGAG 
CGTGTTGTCA TGATGAAATA TGACCGTAAA GTACCAAGTC CAACTGGTGA ATACGTTAAG 
GAACCTGAAT GGGTGTTGGA AACAGATGGT GTTAACTTAT CTGAAGTTAT GACTGTTCCT 
GGTATCGACC CAACCAGAAT CTATACCAAC TCCTTCATTG ATATAATGGA AGTTCTAGGT 
ATTGAAGCTG GTCGTGCAGC CTTGTATAAA GAAGTTTACA ATGTTATTGC TTCTGATGGT 
TCGTATGTTA ACTACCGTCA TATGGCTTTG TTAGTCGATG TTATGACAAC CCAAGGTGGC 
TTAACTTCTG TTACTCGTCA TGGTTTCAAC AGATCAAATA CAGGTGCCTT AATGAGATGT 
TCATTTGAAG AAACTGTCGA AATTTTGTTT GAAGCTGGTG CTTCAGCCGA ATTAGATGAT 
TGTCGTGGTG TTTCGGAAAA TGTCATTCTT GGTCAAATGG CTCCAATCGG TACCGGTGCA 
TTTGATGTGA TGATCGATGA GGAGTCACTG GTAAAATACA TGCCAGAACA AAAAATAACT 
GAGATTGAAG ACGGACAAGA TGGTGGCGTC ACACCATACA GTAACGAAAG TGGTTTGGTC 
AATGCAGATC TTGACGTTAA AGATGAGCTA ATGTTTTCAC CTCTGGTTGA TTCGGGTTCA 
AATGACGCTA TGGCTGGAGG ATTTACAGCG TACGGTGGTG CTGATTATGG TGAAGCCACG 
TCTCCATTTG GTGCTTATGG TGAAGCACCT ACATCTCCCG GATTTGGAGT CTCCTCACCA 
GGCTTTTCTC CAACTTCCCC AACATACTCT CCTACCTCTC CAGCGTACTC ACCAACATCA 
CCATCGTACT CACCAACATC ACCATCGTAC TCGCCAACAT CACCATCGTA CTCACCTACA 
TCACCATCGT ATTCACCAAC GTCACCATCA TATTCGCCAA CGTCACCATC ATATTCGCCA 
ACGTCGCCAT CGTATTCTCC AACGTCACCA TCGTATTCGC CAACGTCGCC TTCCTACTCT 
CCCACGTCGC CAAGCTACAG CCCTACGTCT CCTTCTTATT CTCCTACATC TCCATCATAC 
TCTCCTACGT CACCAAGTTA CAGCCCAACG TCACCAAGTT ACAGCCCAAC GTCTCCAGCC 
TATTCCCCAA CATCACCAAG TTATAGTCCT ACATCGCCTT CATACTCTCC AACATCACCA 
TCCTATTCCC CAACATCACC TTCTTACTCT CCCACCTCTC CAAACTATAG CCCTACTTCA 
CCTTCTTACT CCCCAACATC TCCAGGCTAC AGCCCAGGAT CTCCTGCATA TTCTCCAAAG 
CAAGACGAAC AAAAGCATAA TGAAAATGAA AATTCCAGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.
Nature387(6632 Suppl)75-8(1997 May)
Jacq C,Alt-M?rbe J,Andre B,Arnold W,Bahr A,Ballesta JP,Bargues M,Baron L,Becker A,Biteau N,Bl?cker H,Blugeon C,Boskovic J,Brandt P,Br?ckner M,Buitrago MJ,Coster F,Delaveau T,del Rey F,Dujon B,Eide LG,Garcia-Cantalejo JM,Goffeau A,Gomez-Peris A,Zaccaria P

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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RPO21 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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