SSB1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	SSB1
Entrez Gene ID	851369
Full Name	Hsp70 family ATPase SSB1
Synonyms	YG101
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001180289.1	NP_010052.1	Hsp70 family ATPase SSB1

Pathways from BioSystems

Homologies

Eremothecium gossypii	AGOS_ABL174C	NP_982773.1
Magnaporthe oryzae (rice blast fungus)	MGG_11513	XP_003718330.1
Kluyveromyces lactis	KLLA0D19041g	XP_453906.1
Schizosaccharomyces pombe (fission yeast)	sks2	NP_595438.1
Saccharomyces cerevisiae (baker's yeast)	SSB1	NP_010052.1
Saccharomyces cerevisiae (baker's yeast)	SSB2	NP_014190.1
Neurospora crassa	NCU02075	XP_964602.2

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.
Jacq C, Alt-M?rbe J, Andre B, Arnold W, Bahr A, Ballesta JP, Bargues M, Baron L, Becker A, Biteau N, Bl?cker H, Blugeon C, Boskovic J, Brandt P, Br?ckner M, Buitrago MJ, Coster F, Delaveau T, del Rey F, Dujon B, Eide LG, Garcia-Cantalejo JM, Goffeau A, Gomez-Peris A, Zaccaria P
Nature387(6632 Suppl)75-8(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Study reveals molecular features of chaperone action during translation in eukaryotes by providing proteome-wide Ssb (two isoforms Ssb1 and Ssb2) interaction profiles with nascent chains at near-codon resolution. The Ssb interactome is broader than previously thought and includes nascent mitochondrial and endoplasmic reticulum- (ER) translocated proteins.
Title: Profiling Ssb-Nascent Chain Interactions Reveals Principles of Hsp70-Assisted Folding.

the ribosome-associated Hsp70 Ssb is redistributed away from Sup35 prion aggregates to the nascent chains, leading to an array of aggregation phenotypes that can mimic both overexpression and deletion of Ssb.
Title: Prion-Associated Toxicity is Rescued by Elimination of Cotranslational Chaperones.

Studied deletions of two major chaperone proteins, SSA1 and SSB1, from the HSP70 chaperone network in Sacchromyces cerevisiae.
Title: Quantitative proteomics and network analysis of SSA1 and SSB1 deletion mutants reveals robustness of chaperone HSP70 network in Saccharomyces cerevisiae.

Study finds that SSB (i.e. closely related isoforms Ssb1 and Ssb2) binds to a subset of nascent polypeptides whose intrinsic properties and slow translation rates hinder efficient cotranslational folding.
Title: The cotranslational function of ribosome-associated Hsp70 in eukaryotic protein homeostasis.

The ribosome-bound chaperone system consisting of the ribosome-associated complex (RAC) and the Hsp70 homologs SSB1 and SSB2 are required to stabilize translationally repressed ribosome-polylysine protein complexes.
Title: Ribosome-associated complex and Ssb are required for translational repression induced by polylysine segments within nascent chains.

The following SSB1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SSB1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi00926	NM_001180289.1 Latest version!	Saccharomyces cerevisiae S288C Hsp70 family ATPase SSB1 (SSB1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SSB1 ( NM_001180289.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_010052.1 Saccharomyces cerevisiae S288C Hsp70 family ATPase SSB1 (SSB1), partial mRNA.

Price & Availability↑

CloneID	OSi00926
Clone ID Related Accession (Same CDS sequence)	NM_001180289.1
Accession Version	NM_001180289.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1842bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	Hsp70 family ATPase SSB1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001136). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGGCTGAAG GTGTTTTCCA AGGTGCTATC GGTATCGATT TAGGTACAAC CTACTCTTGT 
GTTGCTACTT ACGAATCCTC CGTTGAAATT ATTGCCAACG AACAAGGTAA CAGAGTCACC 
CCATCTTTCG TTGCTTTCAC TCCAGAAGAA AGATTGATTG GTGATGCTGC CAAGAACCAA 
GCTGCTTTGA ACCCAAGAAA CACTGTCTTC GATGCTAAGC GTTTGATTGG TAGAAGATTC 
GACGACGAAT CTGTTCAAAA GGACATGAAG ACCTGGCCTT TCAAGGTTAT CGACGTCGAT 
GGTAACCCAG TCATCGAAGT CCAATACTTG GAAGAAACCA AGACTTTCTC CCCACAAGAA 
ATTTCCGCTA TGGTTTTGAC CAAGATGAAG GAAATTGCTG AAGCTAAGAT TGGTAAGAAG 
GTTGAAAAGG CCGTCATTAC TGTCCCAGCT TACTTTAACG ACGCTCAAAG ACAAGCTACC 
AAGGATGCCG GTGCCATTTC TGGTTTGAAC GTTTTGCGTA TCATCAACGA ACCTACTGCC 
GCTGCTATTG CTTACGGTCT AGGTGCTGGT AAGTCCGAAA AGGAAAGACA TGTTTTGATT 
TTCGATTTGG GTGGTGGTAC TTTCGATGTT TCCTTGTTGC ACATTGCTGG TGGTGTTTAC 
ACTGTTAAAT CTACTTCCGG TAACACTCAC TTGGGTGGTC AAGATTTCGA CACCAACTTG 
TTGGAACACT TCAAGGCTGA ATTCAAGAAG AAGACTGGTT TGGACATCTC CGACGATGCC 
AGAGCTTTGA GAAGATTGAG AACTGCTGCT GAAAGAGCTA AGAGAACCTT ATCTTCTGTC 
ACTCAAACTA CCGTTGAAGT TGACTCTTTG TTTGACGGTG AAGATTTCGA ATCCTCTTTG 
ACTAGAGCTA GATTTGAAGA CTTGAACGCC GCATTGTTCA AGTCTACTTT GGAACCTGTT 
GAACAAGTTT TGAAGGATGC TAAGATCTCT AAGTCTCAAA TCGACGAAGT TGTCTTGGTT 
GGTGGTTCCA CCAGAATTCC AAAGGTCCAA AAGTTGTTGT CTGACTTCTT TGACGGTAAG 
CAATTGGAAA AATCTATTAA CCCAGATGAA GCTGTTGCTT ACGGTGCTGC TGTTCAAGGT 
GCTATCTTGA CCGGCCAATC CACATCTGAC GAAACCAAGG ACTTGTTGTT GTTAGATGTT 
GCTCCATTAT CTCTAGGTGT TGGTATGCAA GGTGACATGT TCGGTATCGT TGTTCCAAGA 
AACACTACTG TTCCAACCAT CAAGAGAAGA ACCTTTACTA CATGTGCTGA CAACCAAACC 
ACCGTTCAAT TCCCAGTCTA CCAAGGTGAA CGTGTTAACT GTAAAGAAAA CACTTTGTTG 
GGTGAATTCG ACTTGAAGAA CATCCCAATG ATGCCAGCTG GTGAACCAGT CTTGGAAGCT 
ATCTTCGAAG TTGATGCTAA CGGTATCTTG AAGGTTACTG CCGTCGAAAA GTCTACCGGT 
AAGTCTTCTA ACATCACTAT CTCTAACGCT GTTGGTAGAT TGTCTTCTGA AGAAATTGAA 
AAGATGGTTA ACCAAGCTGA AGAGTTCAAG GCTGCCGATG AAGCTTTTGC CAAGAAGCAC 
GAAGCTAGAC AAAGATTGGA ATCCTACGTT GCCTCCATCG AACAAACTGT CACTGACCCA 
GTCTTGTCTT CTAAATTGAA GAGAGGTTCC AAGTCCAAGA TTGAAGCTGC TTTGTCCGAT 
GCTTTGGCTG CTTTGCAAAT CGAAGACCCA TCTGCTGATG AATTGAGAAA GGCTGAAGTT 
GGTTTGAAGA GAGTTGTCAC CAAGGCCATG TCTTCTCGTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_010052.1
CDS	1..1842
Translation	MAEGVFQGAIGIDLGTTYSCVATYESSVEIIANEQGNRVTPSFVAFTPEERLIGDAAKNQAALNPRNTVFDAKRLIGRRFDDESVQKDMKTWPFKVIDVDGNPVIEVQYLEETKTFSPQEISAMVLTKMKEIAEAKIGKKVEKAVITVPAYFNDAQRQATKDAGAISGLNVLRIINEPTAAAIAYGLGAGKSEKERHVLIFDLGGGTFDVSLLHIAGGVYTVKSTSGNTHLGGQDFDTNLLEHFKAEFKKKTGLDISDDARALRRLRTAAERAKRTLSSVTQTTVEVDSLFDGEDFESSLTRARFEDLNAALFKSTLEPVEQVLKDAKISKSQIDEVVLVGGSTRIPKVQKLLSDFFDGKQLEKSINPDEAVAYGAAVQGAILTGQSTSDETKDLLLLDVAPLSLGVGMQGDMFGIVVPRNTTVPTIKRRTFTTCADNQTTVQFPVYQGERVNCKENTLLGEFDLKNIPMMPAGEPVLEAIFEVDANGILKVTAVEKSTGKSSNITISNAVGRLSSEEIEKMVNQAEEFKAADEAFAKKHEARQRLESYVASIEQTVTDPVLSSKLKRGSKSKIEAALSDALAALQIEDPSADELRKAEVGLKRVVTKAMSSR

Target ORF information:

RefSeq Version	NM_001180289.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C Hsp70 family ATPase SSB1 (SSB1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001180289.1

ORF Insert Sequence:

ATGGCTGAAG GTGTTTTCCA AGGTGCTATC GGTATCGATT TAGGTACAAC CTACTCTTGT 
GTTGCTACTT ACGAATCCTC CGTTGAAATT ATTGCCAACG AACAAGGTAA CAGAGTCACC 
CCATCTTTCG TTGCTTTCAC TCCAGAAGAA AGATTGATTG GTGATGCTGC CAAGAACCAA 
GCTGCTTTGA ACCCAAGAAA CACTGTCTTC GATGCTAAGC GTTTGATTGG TAGAAGATTC 
GACGACGAAT CTGTTCAAAA GGACATGAAG ACCTGGCCTT TCAAGGTTAT CGACGTCGAT 
GGTAACCCAG TCATCGAAGT CCAATACTTG GAAGAAACCA AGACTTTCTC CCCACAAGAA 
ATTTCCGCTA TGGTTTTGAC CAAGATGAAG GAAATTGCTG AAGCTAAGAT TGGTAAGAAG 
GTTGAAAAGG CCGTCATTAC TGTCCCAGCT TACTTTAACG ACGCTCAAAG ACAAGCTACC 
AAGGATGCCG GTGCCATTTC TGGTTTGAAC GTTTTGCGTA TCATCAACGA ACCTACTGCC 
GCTGCTATTG CTTACGGTCT AGGTGCTGGT AAGTCCGAAA AGGAAAGACA TGTTTTGATT 
TTCGATTTGG GTGGTGGTAC TTTCGATGTT TCCTTGTTGC ACATTGCTGG TGGTGTTTAC 
ACTGTTAAAT CTACTTCCGG TAACACTCAC TTGGGTGGTC AAGATTTCGA CACCAACTTG 
TTGGAACACT TCAAGGCTGA ATTCAAGAAG AAGACTGGTT TGGACATCTC CGACGATGCC 
AGAGCTTTGA GAAGATTGAG AACTGCTGCT GAAAGAGCTA AGAGAACCTT ATCTTCTGTC 
ACTCAAACTA CCGTTGAAGT TGACTCTTTG TTTGACGGTG AAGATTTCGA ATCCTCTTTG 
ACTAGAGCTA GATTTGAAGA CTTGAACGCC GCATTGTTCA AGTCTACTTT GGAACCTGTT 
GAACAAGTTT TGAAGGATGC TAAGATCTCT AAGTCTCAAA TCGACGAAGT TGTCTTGGTT 
GGTGGTTCCA CCAGAATTCC AAAGGTCCAA AAGTTGTTGT CTGACTTCTT TGACGGTAAG 
CAATTGGAAA AATCTATTAA CCCAGATGAA GCTGTTGCTT ACGGTGCTGC TGTTCAAGGT 
GCTATCTTGA CCGGCCAATC CACATCTGAC GAAACCAAGG ACTTGTTGTT GTTAGATGTT 
GCTCCATTAT CTCTAGGTGT TGGTATGCAA GGTGACATGT TCGGTATCGT TGTTCCAAGA 
AACACTACTG TTCCAACCAT CAAGAGAAGA ACCTTTACTA CATGTGCTGA CAACCAAACC 
ACCGTTCAAT TCCCAGTCTA CCAAGGTGAA CGTGTTAACT GTAAAGAAAA CACTTTGTTG 
GGTGAATTCG ACTTGAAGAA CATCCCAATG ATGCCAGCTG GTGAACCAGT CTTGGAAGCT 
ATCTTCGAAG TTGATGCTAA CGGTATCTTG AAGGTTACTG CCGTCGAAAA GTCTACCGGT 
AAGTCTTCTA ACATCACTAT CTCTAACGCT GTTGGTAGAT TGTCTTCTGA AGAAATTGAA 
AAGATGGTTA ACCAAGCTGA AGAGTTCAAG GCTGCCGATG AAGCTTTTGC CAAGAAGCAC 
GAAGCTAGAC AAAGATTGGA ATCCTACGTT GCCTCCATCG AACAAACTGT CACTGACCCA 
GTCTTGTCTT CTAAATTGAA GAGAGGTTCC AAGTCCAAGA TTGAAGCTGC TTTGTCCGAT 
GCTTTGGCTG CTTTGCAAAT CGAAGACCCA TCTGCTGATG AATTGAGAAA GGCTGAAGTT 
GGTTTGAAGA GAGTTGTCAC CAAGGCCATG TCTTCTCGTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.
Nature387(6632 Suppl)75-8(1997 May)
Jacq C,Alt-M?rbe J,Andre B,Arnold W,Bahr A,Ballesta JP,Bargues M,Baron L,Becker A,Biteau N,Bl?cker H,Blugeon C,Boskovic J,Brandt P,Br?ckner M,Buitrago MJ,Coster F,Delaveau T,del Rey F,Dujon B,Eide LG,Garcia-Cantalejo JM,Goffeau A,Gomez-Peris A,Zaccaria P

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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SSB1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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