FLO1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	FLO1
Entrez Gene ID	851289
Full Name	flocculin FLO1
Synonyms	FLO2,FLO4
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001178230.1	NP_009424.1	flocculin FLO1

Pathways from BioSystems

Homologies

Saccharomyces cerevisiae (baker's yeast)	FLO1	NP_009424.1
Saccharomyces cerevisiae (baker's yeast)	FLO5	NP_012081.1
Saccharomyces cerevisiae (baker's yeast)	TDA8	NP_058136.1
Saccharomyces cerevisiae (baker's yeast)	FLO10	NP_013028.1
Saccharomyces cerevisiae (baker's yeast)	YHR213W	NP_012083.1
Saccharomyces cerevisiae (baker's yeast)	FLO9	NP_009338.1

Gene Ontology
Bibliography

Related articles in PubMed

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

The nucleotide sequence of chromosome I from Saccharomyces cerevisiae.
Bussey H, Kaback DB, Zhong W, Vo DT, Clark MW, Fortin N, Hall J, Ouellette BF, Keng T, Barton AB
Proceedings of the National Academy of Sciences of the United States of America92(9)3809-13(1995 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

we identified a transposable element insertion in the promoter region of known flocculation gene FLO1, and, in an additional five clones, we recovered loss-of-function mutations in transcriptional repressor TUP1, which regulates FLO1 and other related genes
Title: Experimental Evolution Reveals Favored Adaptive Routes to Cell Aggregation in Yeast.

Sas3 and Ada2-dependent histone H3 acetylation is required for transcription elongation at the de-repressed FLO1 gene.
Title: Sas3 and Ada2(Gcn5)-dependent histone H3 acetylation is required for transcription elongation at the de-repressed FLO1 gene.

expression of FLO5 gene was higher than that of FLO1 gene
Title: Genetic diversity of FLO1 and FLO5 genes in wine flocculent Saccharomyces cerevisiae strains.

These data suggest that distinctly bound Cyc8-Tup1 cooperates with Hda1p and Rpd3p to establish or maintain an extensive array of strongly positioned, deacetylated nucleosomes over the FLO1 promoter and upstream region which inhibit histone acetylation, block Swi-Snf binding and prevent transcription.
Title: The yeast Cyc8-Tup1 complex cooperates with Hda1p and Rpd3p histone deacetylases to robustly repress transcription of the subtelomeric FLO1 gene.

Deletion of tandem repeats B, C and D adjacent to the 3'-terminal of FLO1 increases the conformation of flocculin in response to changes of pH and temperature.
Title: [Effect of tandem repeats adjacent to 3'-terminal of FLO1 on the flocculation function of Saccharomyces cerevisiae].

The following FLO1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the FLO1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi00862	NM_001178230.1 Latest version!	Saccharomyces cerevisiae S288C flocculin FLO1 (FLO1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

FLO1 ( NM_001178230.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_009424.1 Saccharomyces cerevisiae S288C flocculin FLO1 (FLO1), partial mRNA.

Price & Availability↑

CloneID	OSi00862
Clone ID Related Accession (Same CDS sequence)	NM_001178230.1
Accession Version	NM_001178230.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4614bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	flocculin FLO1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001133). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGACAATGC CTCATCGCTA TATGTTTTTG GCAGTCTTTA CACTTCTGGC ACTAACTAGT 
GTGGCCTCAG GAGCCACAGA GGCGTGCTTA CCAGCAGGCC AGAGGAAAAG TGGGATGAAT 
ATAAATTTTT ACCAGTATTC ATTGAAAGAT TCCTCCACAT ATTCGAATGC AGCATATATG 
GCTTATGGAT ATGCCTCAAA AACCAAACTA GGTTCTGTCG GAGGACAAAC TGATATCTCG 
ATTGATTATA ATATTCCCTG TGTTAGTTCA TCAGGCACAT TTCCTTGTCC TCAAGAAGAT 
TCCTATGGAA ACTGGGGATG CAAAGGAATG GGTGCTTGTT CTAATAGTCA AGGAATTGCA 
TACTGGAGTA CTGATTTATT TGGTTTCTAT ACTACCCCAA CAAACGTAAC CCTAGAAATG 
ACAGGTTATT TTTTACCACC ACAGACGGGT TCTTACACAT TCAAGTTTGC TACAGTTGAC 
GACTCTGCAA TTCTATCAGT AGGTGGTGCA ACCGCGTTCA ACTGTTGTGC TCAACAGCAA 
CCGCCGATCA CATCAACGAA CTTTACCATT GACGGTATCA AGCCATGGGG TGGAAGTTTG 
CCACCTAATA TCGAAGGAAC CGTCTATATG TACGCTGGCT ACTATTATCC AATGAAGGTT 
GTTTACTCGA ACGCTGTTTC TTGGGGTACA CTTCCAATTA GTGTGACACT TCCAGATGGT 
ACCACTGTAA GTGATGACTT CGAAGGGTAC GTCTATTCCT TTGACGATGA CCTAAGTCAA 
TCTAACTGTA CTGTCCCTGA CCCTTCAAAT TATGCTGTCA GTACCACTAC AACTACAACG 
GAACCATGGA CCGGTACTTT CACTTCTACA TCTACTGAAA TGACCACCGT CACCGGTACC 
AACGGCGTTC CAACTGACGA AACCGTCATT GTCATCAGAA CTCCAACAAC TGCTAGCACC 
ATCATAACTA CAACTGAGCC ATGGAACAGC ACTTTTACCT CTACTTCTAC CGAATTGACC 
ACAGTCACTG GCACCAATGG TGTACGAACT GACGAAACCA TCATTGTAAT CAGAACACCA 
ACAACAGCCA CTACTGCCAT AACTACAACT GAGCCATGGA ACAGCACTTT TACCTCTACT 
TCTACCGAAT TGACCACAGT CACCGGTACC AATGGTTTGC CAACTGATGA GACCATCATT 
GTCATCAGAA CACCAACAAC AGCCACTACT GCCATGACTA CAACTCAGCC ATGGAACGAC 
ACTTTTACCT CTACTTCTAC CGAATTGACC ACAGTCACCG GTACCAATGG TTTGCCAACT 
GATGAGACCA TCATTGTCAT CAGAACACCA ACAACAGCCA CTACTGCCAT GACTACAACT 
CAGCCATGGA ACGACACTTT TACCTCTACT TCTACCGAAT TGACCACAGT CACCGGTACC 
AATGGTTTGC CAACTGATGA GACCATCATT GTCATCAGAA CACCAACAAC AGCCACTACT 
GCCATGACTA CAACTCAGCC ATGGAACGAC ACTTTTACCT CTACATCCAC TGAAATCACC 
ACCGTCACCG GTACCAATGG TTTGCCAACT GATGAGACCA TCATTGTCAT CAGAACACCA 
ACAACAGCCA CTACTGCCAT GACTACACCT CAGCCATGGA ACGACACTTT TACCTCTACA 
TCCACTGAAA TGACCACCGT CACCGGTACC AACGGTTTGC CAACTGATGA AACCATCATT 
GTCATCAGAA CACCAACAAC AGCCACTACT GCCATAACTA CAACTGAGCC ATGGAACAGC 
ACTTTTACCT CTACATCCAC TGAAATGACC ACCGTCACCG GTACCAACGG TTTGCCAACT 
GATGAAACCA TCATTGTCAT CAGAACACCA ACAACAGCCA CTACTGCCAT AACTACAACT 
CAGCCATGGA ACGACACTTT TACCTCTACA TCCACTGAAA TGACCACCGT CACCGGTACC 
AACGGTTTGC CAACTGATGA AACCATCATT GTCATCAGAA CACCAACAAC AGCCACTACT 
GCCATGACTA CAACTCAGCC ATGGAACGAC ACTTTTACCT CTACATCCAC TGAAATCACC 
ACCGTCACCG GTACCACCGG TTTGCCAACT GATGAGACCA TCATTGTCAT CAGAACACCA 
ACAACAGCCA CTACTGCCAT GACTACAACT CAGCCATGGA ACGACACTTT TACCTCTACA 
TCCACTGAAA TGACCACCGT CACCGGTACC AACGGCGTTC CAACTGACGA AACCGTCATT 
GTCATCAGAA CTCCAACTAG TGAAGGTCTA ATCAGCACCA CCACTGAACC ATGGACTGGT 
ACTTTCACCT CTACATCCAC TGAGATGACC ACCGTCACCG GTACTAACGG TCAACCAACT 
GACGAAACCG TGATTGTTAT CAGAACTCCA ACCAGTGAAG GTTTGGTTAC AACCACCACT 
GAACCATGGA CTGGTACTTT TACTTCTACA TCTACTGAAA TGACCACCAT TACTGGAACC 
AACGGCGTTC CAACTGACGA AACCGTCATT GTCATCAGAA CTCCAACCAG TGAAGGTCTA 
ATCAGCACCA CCACTGAACC ATGGACTGGT ACTTTTACTT CTACATCTAC TGAAATGACC 
ACCATTACTG GAACCAATGG TCAACCAACT GACGAAACCG TTATTGTTAT CAGAACTCCA 
ACTAGTGAAG GTCTAATCAG CACTACAACG GAACCATGGA CCGGTACTTT CACTTCTACA 
TCTACTGAAA TGACGCACGT CACCGGTACC AACGGCGTTC CAACTGACGA AACCGTCATT 
GTCATCAGAA CTCCAACCAG TGAAGGTCTA ATCAGCACCA CCACTGAACC ATGGACTGGC 
ACTTTCACTT CGACTTCCAC TGAGGTTACC ACCATCACTG GAACCAACGG TCAACCAACT 
GACGAAACTG TGATTGTTAT CAGAACTCCA ACCAGTGAAG GTCTAATCAG CACCACCACT 
GAACCATGGA CTGGTACTTT CACTTCTACA TCTACTGAAA TGACCACCGT CACCGGTACT 
AACGGTCAAC CAACTGACGA AACCGTGATT GTTATCAGAA CTCCAACCAG TGAAGGTTTG 
GTTACAACCA CCACTGAACC ATGGACTGGT ACTTTTACTT CGACTTCCAC TGAAATGTCT 
ACTGTCACTG GAACCAATGG CTTGCCAACT GATGAAACTG TCATTGTTGT CAAAACTCCA 
ACTACTGCCA TCTCATCCAG TTTGTCATCA TCATCTTCAG GACAAATCAC CAGCTCTATC 
ACGTCTTCGC GTCCAATTAT TACCCCATTC TATCCTAGCA ATGGAACTTC TGTGATTTCT 
TCCTCAGTAA TTTCTTCCTC AGTCACTTCT TCTCTATTCA CTTCTTCTCC AGTCATTTCT 
TCCTCAGTCA TTTCTTCTTC TACAACAACC TCCACTTCTA TATTTTCTGA ATCATCTAAA 
TCATCCGTCA TTCCAACCAG TAGTTCCACC TCTGGTTCTT CTGAGAGCGA AACGAGTTCA 
GCTGGTTCTG TCTCTTCTTC CTCTTTTATC TCTTCTGAAT CATCAAAATC TCCTACATAT 
TCTTCTTCAT CATTACCACT TGTTACCAGT GCGACAACAA GCCAGGAAAC TGCTTCTTCA 
TTACCACCTG CTACCACTAC AAAAACGAGC GAACAAACCA CTTTGGTTAC CGTGACATCC 
TGCGAGTCTC ATGTGTGCAC TGAATCCATC TCCCCTGCGA TTGTTTCCAC AGCTACTGTT 
ACTGTTAGCG GCGTCACAAC AGAGTATACC ACATGGTGCC CTATTTCTAC TACAGAGACA 
ACAAAGCAAA CCAAAGGGAC AACAGAGCAA ACCACAGAAA CAACAAAACA AACCACGGTA 
GTTACAATTT CTTCTTGTGA ATCTGACGTA TGCTCTAAGA CTGCTTCTCC AGCCATTGTA 
TCTACAAGCA CTGCTACTAT TAACGGCGTT ACTACAGAAT ACACAACATG GTGTCCTATT 
TCCACCACAG AATCGAGGCA ACAAACAACG CTAGTTACTG TTACTTCCTG CGAATCTGGT 
GTGTGTTCCG AAACTGCTTC ACCTGCCATT GTTTCGACGG CCACGGCTAC TGTGAATGAT 
GTTGTTACGG TCTATCCTAC ATGGAGGCCA CAGACTGCGA ATGAAGAGTC TGTCAGCTCT 
AAAATGAACA GTGCTACCGG TGAGACAACA ACCAATACTT TAGCTGCTGA AACGACTACC 
AATACTGTAG CTGCTGAGAC GATTACCAAT ACTGGAGCTG CTGAGACGAA AACAGTAGTC 
ACCTCTTCGC TTTCAAGATC TAATCACGCT GAAACACAGA CGGCTTCCGC GACCGATGTG 
ATTGGTCACA GCAGTAGTGT TGTTTCTGTA TCCGAAACTG GCAACACCAA GAGTCTAACA 
AGTTCCGGGT TGAGTACTAT GTCGCAACAG CCTCGTAGCA CACCAGCAAG CAGCATGGTA 
GGATATAGTA CAGCTTCTTT AGAAATTTCA ACGTATGCTG GCAGTGCCAA CAGCTTACTG 
GCCGGTAGTG GTTTAAGTGT CTTCATTGCG TCCTTATTGC TGGCAATTAT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_009424.1
CDS	1..4614
Translation	MTMPHRYMFLAVFTLLALTSVASGATEACLPAGQRKSGMNINFYQYSLKDSSTYSNAAYMAYGYASKTKLGSVGGQTDISIDYNIPCVSSSGTFPCPQEDSYGNWGCKGMGACSNSQGIAYWSTDLFGFYTTPTNVTLEMTGYFLPPQTGSYTFKFATVDDSAILSVGGATAFNCCAQQQPPITSTNFTIDGIKPWGGSLPPNIEGTVYMYAGYYYPMKVVYSNAVSWGTLPISVTLPDGTTVSDDFEGYVYSFDDDLSQSNCTVPDPSNYAVSTTTTTTEPWTGTFTSTSTEMTTVTGTNGVPTDETVIVIRTPTTASTIITTTEPWNSTFTSTSTELTTVTGTNGVRTDETIIVIRTPTTATTAITTTEPWNSTFTSTSTELTTVTGTNGLPTDETIIVIRTPTTATTAMTTTQPWNDTFTSTSTELTTVTGTNGLPTDETIIVIRTPTTATTAMTTTQPWNDTFTSTSTELTTVTGTNGLPTDETIIVIRTPTTATTAMTTTQPWNDTFTSTSTEITTVTGTNGLPTDETIIVIRTPTTATTAMTTPQPWNDTFTSTSTEMTTVTGTNGLPTDETIIVIRTPTTATTAITTTEPWNSTFTSTSTEMTTVTGTNGLPTDETIIVIRTPTTATTAITTTQPWNDTFTSTSTEMTTVTGTNGLPTDETIIVIRTPTTATTAMTTTQPWNDTFTSTSTEITTVTGTTGLPTDETIIVIRTPTTATTAMTTTQPWNDTFTSTSTEMTTVTGTNGVPTDETVIVIRTPTSEGLISTTTEPWTGTFTSTSTEMTTVTGTNGQPTDETVIVIRTPTSEGLVTTTTEPWTGTFTSTSTEMTTITGTNGVPTDETVIVIRTPTSEGLISTTTEPWTGTFTSTSTEMTTITGTNGQPTDETVIVIRTPTSEGLISTTTEPWTGTFTSTSTEMTHVTGTNGVPTDETVIVIRTPTSEGLISTTTEPWTGTFTSTSTEVTTITGTNGQPTDETVIVIRTPTSEGLISTTTEPWTGTFTSTSTEMTTVTGTNGQPTDETVIVIRTPTSEGLVTTTTEPWTGTFTSTSTEMSTVTGTNGLPTDETVIVVKTPTTAISSSLSSSSSGQITSSITSSRPIITPFYPSNGTSVISSSVISSSVTSSLFTSSPVISSSVISSSTTTSTSIFSESSKSSVIPTSSSTSGSSESETSSAGSVSSSSFISSESSKSPTYSSSSLPLVTSATTSQETASSLPPATTTKTSEQTTLVTVTSCESHVCTESISPAIVSTATVTVSGVTTEYTTWCPISTTETTKQTKGTTEQTTETTKQTTVVTISSCESDVCSKTASPAIVSTSTATINGVTTEYTTWCPISTTESRQQTTLVTVTSCESGVCSETASPAIVSTATATVNDVVTVYPTWRPQTANEESVSSKMNSATGETTTNTLAAETTTNTVAAETITNTGAAETKTVVTSSLSRSNHAETQTASATDVIGHSSSVVSVSETGNTKSLTSSGLSTMSQQPRSTPASSMVGYSTASLEISTYAGSANSLLAGSGLSVFIASLLLAII

Target ORF information:

RefSeq Version	NM_001178230.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C flocculin FLO1 (FLO1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001178230.1

ORF Insert Sequence:

ATGACAATGC CTCATCGCTA TATGTTTTTG GCAGTCTTTA CACTTCTGGC ACTAACTAGT 
GTGGCCTCAG GAGCCACAGA GGCGTGCTTA CCAGCAGGCC AGAGGAAAAG TGGGATGAAT 
ATAAATTTTT ACCAGTATTC ATTGAAAGAT TCCTCCACAT ATTCGAATGC AGCATATATG 
GCTTATGGAT ATGCCTCAAA AACCAAACTA GGTTCTGTCG GAGGACAAAC TGATATCTCG 
ATTGATTATA ATATTCCCTG TGTTAGTTCA TCAGGCACAT TTCCTTGTCC TCAAGAAGAT 
TCCTATGGAA ACTGGGGATG CAAAGGAATG GGTGCTTGTT CTAATAGTCA AGGAATTGCA 
TACTGGAGTA CTGATTTATT TGGTTTCTAT ACTACCCCAA CAAACGTAAC CCTAGAAATG 
ACAGGTTATT TTTTACCACC ACAGACGGGT TCTTACACAT TCAAGTTTGC TACAGTTGAC 
GACTCTGCAA TTCTATCAGT AGGTGGTGCA ACCGCGTTCA ACTGTTGTGC TCAACAGCAA 
CCGCCGATCA CATCAACGAA CTTTACCATT GACGGTATCA AGCCATGGGG TGGAAGTTTG 
CCACCTAATA TCGAAGGAAC CGTCTATATG TACGCTGGCT ACTATTATCC AATGAAGGTT 
GTTTACTCGA ACGCTGTTTC TTGGGGTACA CTTCCAATTA GTGTGACACT TCCAGATGGT 
ACCACTGTAA GTGATGACTT CGAAGGGTAC GTCTATTCCT TTGACGATGA CCTAAGTCAA 
TCTAACTGTA CTGTCCCTGA CCCTTCAAAT TATGCTGTCA GTACCACTAC AACTACAACG 
GAACCATGGA CCGGTACTTT CACTTCTACA TCTACTGAAA TGACCACCGT CACCGGTACC 
AACGGCGTTC CAACTGACGA AACCGTCATT GTCATCAGAA CTCCAACAAC TGCTAGCACC 
ATCATAACTA CAACTGAGCC ATGGAACAGC ACTTTTACCT CTACTTCTAC CGAATTGACC 
ACAGTCACTG GCACCAATGG TGTACGAACT GACGAAACCA TCATTGTAAT CAGAACACCA 
ACAACAGCCA CTACTGCCAT AACTACAACT GAGCCATGGA ACAGCACTTT TACCTCTACT 
TCTACCGAAT TGACCACAGT CACCGGTACC AATGGTTTGC CAACTGATGA GACCATCATT 
GTCATCAGAA CACCAACAAC AGCCACTACT GCCATGACTA CAACTCAGCC ATGGAACGAC 
ACTTTTACCT CTACTTCTAC CGAATTGACC ACAGTCACCG GTACCAATGG TTTGCCAACT 
GATGAGACCA TCATTGTCAT CAGAACACCA ACAACAGCCA CTACTGCCAT GACTACAACT 
CAGCCATGGA ACGACACTTT TACCTCTACT TCTACCGAAT TGACCACAGT CACCGGTACC 
AATGGTTTGC CAACTGATGA GACCATCATT GTCATCAGAA CACCAACAAC AGCCACTACT 
GCCATGACTA CAACTCAGCC ATGGAACGAC ACTTTTACCT CTACATCCAC TGAAATCACC 
ACCGTCACCG GTACCAATGG TTTGCCAACT GATGAGACCA TCATTGTCAT CAGAACACCA 
ACAACAGCCA CTACTGCCAT GACTACACCT CAGCCATGGA ACGACACTTT TACCTCTACA 
TCCACTGAAA TGACCACCGT CACCGGTACC AACGGTTTGC CAACTGATGA AACCATCATT 
GTCATCAGAA CACCAACAAC AGCCACTACT GCCATAACTA CAACTGAGCC ATGGAACAGC 
ACTTTTACCT CTACATCCAC TGAAATGACC ACCGTCACCG GTACCAACGG TTTGCCAACT 
GATGAAACCA TCATTGTCAT CAGAACACCA ACAACAGCCA CTACTGCCAT AACTACAACT 
CAGCCATGGA ACGACACTTT TACCTCTACA TCCACTGAAA TGACCACCGT CACCGGTACC 
AACGGTTTGC CAACTGATGA AACCATCATT GTCATCAGAA CACCAACAAC AGCCACTACT 
GCCATGACTA CAACTCAGCC ATGGAACGAC ACTTTTACCT CTACATCCAC TGAAATCACC 
ACCGTCACCG GTACCACCGG TTTGCCAACT GATGAGACCA TCATTGTCAT CAGAACACCA 
ACAACAGCCA CTACTGCCAT GACTACAACT CAGCCATGGA ACGACACTTT TACCTCTACA 
TCCACTGAAA TGACCACCGT CACCGGTACC AACGGCGTTC CAACTGACGA AACCGTCATT 
GTCATCAGAA CTCCAACTAG TGAAGGTCTA ATCAGCACCA CCACTGAACC ATGGACTGGT 
ACTTTCACCT CTACATCCAC TGAGATGACC ACCGTCACCG GTACTAACGG TCAACCAACT 
GACGAAACCG TGATTGTTAT CAGAACTCCA ACCAGTGAAG GTTTGGTTAC AACCACCACT 
GAACCATGGA CTGGTACTTT TACTTCTACA TCTACTGAAA TGACCACCAT TACTGGAACC 
AACGGCGTTC CAACTGACGA AACCGTCATT GTCATCAGAA CTCCAACCAG TGAAGGTCTA 
ATCAGCACCA CCACTGAACC ATGGACTGGT ACTTTTACTT CTACATCTAC TGAAATGACC 
ACCATTACTG GAACCAATGG TCAACCAACT GACGAAACCG TTATTGTTAT CAGAACTCCA 
ACTAGTGAAG GTCTAATCAG CACTACAACG GAACCATGGA CCGGTACTTT CACTTCTACA 
TCTACTGAAA TGACGCACGT CACCGGTACC AACGGCGTTC CAACTGACGA AACCGTCATT 
GTCATCAGAA CTCCAACCAG TGAAGGTCTA ATCAGCACCA CCACTGAACC ATGGACTGGC 
ACTTTCACTT CGACTTCCAC TGAGGTTACC ACCATCACTG GAACCAACGG TCAACCAACT 
GACGAAACTG TGATTGTTAT CAGAACTCCA ACCAGTGAAG GTCTAATCAG CACCACCACT 
GAACCATGGA CTGGTACTTT CACTTCTACA TCTACTGAAA TGACCACCGT CACCGGTACT 
AACGGTCAAC CAACTGACGA AACCGTGATT GTTATCAGAA CTCCAACCAG TGAAGGTTTG 
GTTACAACCA CCACTGAACC ATGGACTGGT ACTTTTACTT CGACTTCCAC TGAAATGTCT 
ACTGTCACTG GAACCAATGG CTTGCCAACT GATGAAACTG TCATTGTTGT CAAAACTCCA 
ACTACTGCCA TCTCATCCAG TTTGTCATCA TCATCTTCAG GACAAATCAC CAGCTCTATC 
ACGTCTTCGC GTCCAATTAT TACCCCATTC TATCCTAGCA ATGGAACTTC TGTGATTTCT 
TCCTCAGTAA TTTCTTCCTC AGTCACTTCT TCTCTATTCA CTTCTTCTCC AGTCATTTCT 
TCCTCAGTCA TTTCTTCTTC TACAACAACC TCCACTTCTA TATTTTCTGA ATCATCTAAA 
TCATCCGTCA TTCCAACCAG TAGTTCCACC TCTGGTTCTT CTGAGAGCGA AACGAGTTCA 
GCTGGTTCTG TCTCTTCTTC CTCTTTTATC TCTTCTGAAT CATCAAAATC TCCTACATAT 
TCTTCTTCAT CATTACCACT TGTTACCAGT GCGACAACAA GCCAGGAAAC TGCTTCTTCA 
TTACCACCTG CTACCACTAC AAAAACGAGC GAACAAACCA CTTTGGTTAC CGTGACATCC 
TGCGAGTCTC ATGTGTGCAC TGAATCCATC TCCCCTGCGA TTGTTTCCAC AGCTACTGTT 
ACTGTTAGCG GCGTCACAAC AGAGTATACC ACATGGTGCC CTATTTCTAC TACAGAGACA 
ACAAAGCAAA CCAAAGGGAC AACAGAGCAA ACCACAGAAA CAACAAAACA AACCACGGTA 
GTTACAATTT CTTCTTGTGA ATCTGACGTA TGCTCTAAGA CTGCTTCTCC AGCCATTGTA 
TCTACAAGCA CTGCTACTAT TAACGGCGTT ACTACAGAAT ACACAACATG GTGTCCTATT 
TCCACCACAG AATCGAGGCA ACAAACAACG CTAGTTACTG TTACTTCCTG CGAATCTGGT 
GTGTGTTCCG AAACTGCTTC ACCTGCCATT GTTTCGACGG CCACGGCTAC TGTGAATGAT 
GTTGTTACGG TCTATCCTAC ATGGAGGCCA CAGACTGCGA ATGAAGAGTC TGTCAGCTCT 
AAAATGAACA GTGCTACCGG TGAGACAACA ACCAATACTT TAGCTGCTGA AACGACTACC 
AATACTGTAG CTGCTGAGAC GATTACCAAT ACTGGAGCTG CTGAGACGAA AACAGTAGTC 
ACCTCTTCGC TTTCAAGATC TAATCACGCT GAAACACAGA CGGCTTCCGC GACCGATGTG 
ATTGGTCACA GCAGTAGTGT TGTTTCTGTA TCCGAAACTG GCAACACCAA GAGTCTAACA 
AGTTCCGGGT TGAGTACTAT GTCGCAACAG CCTCGTAGCA CACCAGCAAG CAGCATGGTA 
GGATATAGTA CAGCTTCTTT AGAAATTTCA ACGTATGCTG GCAGTGCCAA CAGCTTACTG 
GCCGGTAGTG GTTTAAGTGT CTTCATTGCG TCCTTATTGC TGGCAATTAT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

The nucleotide sequence of chromosome I from Saccharomyces cerevisiae.
Proceedings of the National Academy of Sciences of the United States of America92(9)3809-13(1995 Apr)
Bussey H,Kaback DB,Zhong W,Vo DT,Clark MW,Fortin N,Hall J,Ouellette BF,Keng T,Barton AB

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FLO1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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