SST2 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	SST2
Entrez Gene ID	851173
Full Name	GTPase-activating protein SST2
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001182340.1	NP_013557.1	GTPase-activating protein SST2

Pathways from BioSystems

Homologies

Saccharomyces cerevisiae (baker's yeast)	SST2	NP_013557.1
Eremothecium gossypii	AGOS_ABR176C	NP_983124.2
Kluyveromyces lactis	KLLA0D10549g	XP_453531.1

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XII.
Johnston M, Hillier L, Riles L, Albermann K, Andr? B, Ansorge W, Benes V, Br?ckner M, Delius H, Dubois E, D?sterh?ft A, Entian KD, Floeth M, Goffeau A, Hebling U, Heumann K, Heuss-Neitzel D, Hilbert H, Hilger F, Kleine K, K?tter P, Louis EJ, Messenguy F, Mewes HW, Hoheisel JD
Nature387(6632 Suppl)87-90(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Results suggest that the G-protein-coupled receptor, Ste2 binds the inhibitory G-protein signaling, Sst2 forming a complex with antagonistic functions.
Title: Yeast GPCR signaling reflects the fraction of occupied receptors, not the number.

Results reveal that a regulator of G protein signaling can also regulate the G protein-coupled receptor. Whereas Sst2 negatively regulates G protein signaling, it acts in a positive manner to promote receptor retention at the growing edge.
Title: Modulation of receptor dynamics by the regulator of G protein signaling Sst2.

Sst2 limits variability in transcription and morphogenesis in response to pheromone stimulation.
Title: Cellular noise suppression by the regulator of G protein signaling Sst2.

We demonstrate that DEP domains in Sst2 mediate binding to its cognate GPCR (Ste2). DEP-domain-mediated tethering promotes downregulation by placing the RGS protein in proximity to its substrate.
Title: DEP-domain-mediated regulation of GPCR signaling responses.

These findings suggest that Sst2 is the principal regulator of Gpa1-mediated signaling in vivo but that other proteins also contribute in distinct ways to pathway regulation.
Title: Genome-scale analysis reveals Sst2 as the principal regulator of mating pheromone signaling in the yeast Saccharomyces cerevisiae.

The following SST2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SST2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi00764	NM_001182340.1 Latest version!	Saccharomyces cerevisiae S288C GTPase-activating protein SST2 (SST2), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SST2 ( NM_001182340.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_013557.1 Saccharomyces cerevisiae S288C GTPase-activating protein SST2 (SST2), partial mRNA.

Price & Availability↑

CloneID	OSi00764
Clone ID Related Accession (Same CDS sequence)	NM_001182340.1
Accession Version	NM_001182340.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2097bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	GTPase-activating protein SST2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001144). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGGTGGATA AAAATAGGAC GTTGCATGAA TTATCTTCCA AAAATTTCAG CAGAACGCCG 
AATGGACTGA TCTTTACCAA TGATTTAAAG ACAGTATATT CCATTTTTCT AATTTGTTTG 
GATTTGAAAG AGAAAAAACA TAGTAGTGAC ACTAAGTCTT TCTTATTAAC TGCATTCACT 
AAACATTTCC ACTTCACTTT TACATATCAG GAAGCTATTA AAGCAATGGG ACAATTGGAA 
CTAAAAGTGG ATATGAACAC AACTTGTATT AACGTTTCAT ATAATATTAA ACCTAGTTTG 
GCGCGCCACC TTCTTACATT ATTTATGTCT TCTAAGCTAC TACATACACC GCAGGACAGA 
ACTCGTGGCG AGCCAAAAGA GAAAGTTCTT TTCCAACCAA CACCTAAGGG AGTTGCTGTT 
TTACAAAAGT ATGTTAGAGA TATTGGGTTG AAAACAATGC CTGATATTCT TTTGTCATCG 
TTTAACTCCA TGAAACTTTT CACTTTTGAG AGAAGCTCTG TCACGGATTC AATCATCCAT 
AGTGATTATT TGATCCATAT TCTTTTCATT AAAATGATGG GCGCTAAGCC AAATGTTTGG 
TCTCCAACAA ACGCAGATGA CCCATTACCT TGCCTATCTT CGTTGTTGGA ATACACTAAT 
AATGATGATA CATTTACTTT TGAGAAATCT AAGCCAGAAC AAGGATGGCA AGCTCAGATT 
GGAAACATCG ATATAAATGA CTTAGAAAGG GTATCGCCAT TAGCTCATCG ATTCTTTACA 
AATCCAGATT CTGAGTCGCA CACGCAATAC TACGTTTCGA ATGCAGGTAT TAGGTTGTTT 
GAAAATAAGA CGTTTGGCAC TTCCAAAAAA ATCGTGATTA AGTACACCTT TACAACAAAG 
GCTATATGGC AGTGGATAAT GGACTGTACT GATATTATGC ATGTTAAAGA AGCGGTCTCG 
TTGGCAGCGT TATTTTTAAA AACGGGGTTG ATTGTACCAG TTCTTTTACA ACCATCACGC 
ACTGACAAGA AAAAGTTTCA AATTAGCAGG TCCTCTTTTT TTACCTTGTC CAAGCGTGGT 
TGGGATTTGG TTTCTTGGAC AGGTTGTAAG AGCAATAATA TTAGAGCACC AAATGGTAGC 
ACCATAGACC TAGATTTCAC ATTAAGGGGT CATATGACTG TTCGTGATGA AAAAAAGACA 
TTGGATGATA GTGAAGGTTT TTCTCAAGAC ATGCTTATCT CTTCGAGTAA TTTAAATAAA 
CTGGACTATG TGCTAACTGA TCCAGGTATG AGATATTTAT TCAGGCGCCA TTTAGAAAAG 
GAACTTTGTG TAGAAAATTT GGATGTCTTC ATTGAGATTA AGAGGTTTTT GAAGAAAATG 
ACAATTCTGA AAAAATTGAT AGATTCTAAG CATTGTGATA AAAAATCTAA TACCAGTACT 
TCAAAAAACA ATATCGTAAA AACGATTGAT TCAGCTTTAA TGAAACAGGC CAATGAGTGC 
CTTGAAATGG CATATCACAT TTATTCGTCC TATATCATGA TAGGATCACC ATATCAATTA 
AATATCCATC ATAACTTGCG TCAGAATATT TCTGACATCA TGTTGCACCC TCATTCTCCA 
TTATCGGAAC ACTTTCCTAC TAATCTATAC GATCCATCAC CTGCATCCGC GGAATCAGCA 
GCTTCATCAA TTAGCTCAAC GGAGGCTGAC ACGTTAGGTG AACCACCTGA AGTGAGTCTA 
AAACCTTCAA AAAATCTTTC AAATGAAAAC TGTTCATTTA AGAAGCAAGG ATTCAAACAT 
CAGTTAAAGG AATATAAACC TGCCCCGTTA ACTCTGGCAG AAACGCATTC GCCTAATGCA 
TCAGTAGAAA ATTCACATAC AATTGTTCGT TATGGCATGG ACAACACCCA AAATGATACC 
AAATCGGTTG AATCATTTCC AGCAACTTTG AAGGTCCTCA GGAAATTATA TCCCCTCTTC 
GAGATCGTTA GCAATGAAAT GTATAGACTG ATGAATAACG ATTCCTTTCA AAAATTTACC 
CAGTCAGACG TATACAAAGA TGCTAGCGCT TTAATAGAAA TCCAAGAAAA GTGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_013557.1
CDS	1..2097
Translation	MVDKNRTLHELSSKNFSRTPNGLIFTNDLKTVYSIFLICLDLKEKKHSSDTKSFLLTAFTKHFHFTFTYQEAIKAMGQLELKVDMNTTCINVSYNIKPSLARHLLTLFMSSKLLHTPQDRTRGEPKEKVLFQPTPKGVAVLQKYVRDIGLKTMPDILLSSFNSMKLFTFERSSVTDSIIHSDYLIHILFIKMMGAKPNVWSPTNADDPLPCLSSLLEYTNNDDTFTFEKSKPEQGWQAQIGNIDINDLERVSPLAHRFFTNPDSESHTQYYVSNAGIRLFENKTFGTSKKIVIKYTFTTKAIWQWIMDCTDIMHVKEAVSLAALFLKTGLIVPVLLQPSRTDKKKFQISRSSFFTLSKRGWDLVSWTGCKSNNIRAPNGSTIDLDFTLRGHMTVRDEKKTLDDSEGFSQDMLISSSNLNKLDYVLTDPGMRYLFRRHLEKELCVENLDVFIEIKRFLKKMTILKKLIDSKHCDKKSNTSTSKNNIVKTIDSALMKQANECLEMAYHIYSSYIMIGSPYQLNIHHNLRQNISDIMLHPHSPLSEHFPTNLYDPSPASAESAASSISSTEADTLGEPPEVSLKPSKNLSNENCSFKKQGFKHQLKEYKPAPLTLAETHSPNASVENSHTIVRYGMDNTQNDTKSVESFPATLKVLRKLYPLFEIVSNEMYRLMNNDSFQKFTQSDVYKDASALIEIQEKC

Target ORF information:

RefSeq Version	NM_001182340.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C GTPase-activating protein SST2 (SST2), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001182340.1

ORF Insert Sequence:

ATGGTGGATA AAAATAGGAC GTTGCATGAA TTATCTTCCA AAAATTTCAG CAGAACGCCG 
AATGGACTGA TCTTTACCAA TGATTTAAAG ACAGTATATT CCATTTTTCT AATTTGTTTG 
GATTTGAAAG AGAAAAAACA TAGTAGTGAC ACTAAGTCTT TCTTATTAAC TGCATTCACT 
AAACATTTCC ACTTCACTTT TACATATCAG GAAGCTATTA AAGCAATGGG ACAATTGGAA 
CTAAAAGTGG ATATGAACAC AACTTGTATT AACGTTTCAT ATAATATTAA ACCTAGTTTG 
GCGCGCCACC TTCTTACATT ATTTATGTCT TCTAAGCTAC TACATACACC GCAGGACAGA 
ACTCGTGGCG AGCCAAAAGA GAAAGTTCTT TTCCAACCAA CACCTAAGGG AGTTGCTGTT 
TTACAAAAGT ATGTTAGAGA TATTGGGTTG AAAACAATGC CTGATATTCT TTTGTCATCG 
TTTAACTCCA TGAAACTTTT CACTTTTGAG AGAAGCTCTG TCACGGATTC AATCATCCAT 
AGTGATTATT TGATCCATAT TCTTTTCATT AAAATGATGG GCGCTAAGCC AAATGTTTGG 
TCTCCAACAA ACGCAGATGA CCCATTACCT TGCCTATCTT CGTTGTTGGA ATACACTAAT 
AATGATGATA CATTTACTTT TGAGAAATCT AAGCCAGAAC AAGGATGGCA AGCTCAGATT 
GGAAACATCG ATATAAATGA CTTAGAAAGG GTATCGCCAT TAGCTCATCG ATTCTTTACA 
AATCCAGATT CTGAGTCGCA CACGCAATAC TACGTTTCGA ATGCAGGTAT TAGGTTGTTT 
GAAAATAAGA CGTTTGGCAC TTCCAAAAAA ATCGTGATTA AGTACACCTT TACAACAAAG 
GCTATATGGC AGTGGATAAT GGACTGTACT GATATTATGC ATGTTAAAGA AGCGGTCTCG 
TTGGCAGCGT TATTTTTAAA AACGGGGTTG ATTGTACCAG TTCTTTTACA ACCATCACGC 
ACTGACAAGA AAAAGTTTCA AATTAGCAGG TCCTCTTTTT TTACCTTGTC CAAGCGTGGT 
TGGGATTTGG TTTCTTGGAC AGGTTGTAAG AGCAATAATA TTAGAGCACC AAATGGTAGC 
ACCATAGACC TAGATTTCAC ATTAAGGGGT CATATGACTG TTCGTGATGA AAAAAAGACA 
TTGGATGATA GTGAAGGTTT TTCTCAAGAC ATGCTTATCT CTTCGAGTAA TTTAAATAAA 
CTGGACTATG TGCTAACTGA TCCAGGTATG AGATATTTAT TCAGGCGCCA TTTAGAAAAG 
GAACTTTGTG TAGAAAATTT GGATGTCTTC ATTGAGATTA AGAGGTTTTT GAAGAAAATG 
ACAATTCTGA AAAAATTGAT AGATTCTAAG CATTGTGATA AAAAATCTAA TACCAGTACT 
TCAAAAAACA ATATCGTAAA AACGATTGAT TCAGCTTTAA TGAAACAGGC CAATGAGTGC 
CTTGAAATGG CATATCACAT TTATTCGTCC TATATCATGA TAGGATCACC ATATCAATTA 
AATATCCATC ATAACTTGCG TCAGAATATT TCTGACATCA TGTTGCACCC TCATTCTCCA 
TTATCGGAAC ACTTTCCTAC TAATCTATAC GATCCATCAC CTGCATCCGC GGAATCAGCA 
GCTTCATCAA TTAGCTCAAC GGAGGCTGAC ACGTTAGGTG AACCACCTGA AGTGAGTCTA 
AAACCTTCAA AAAATCTTTC AAATGAAAAC TGTTCATTTA AGAAGCAAGG ATTCAAACAT 
CAGTTAAAGG AATATAAACC TGCCCCGTTA ACTCTGGCAG AAACGCATTC GCCTAATGCA 
TCAGTAGAAA ATTCACATAC AATTGTTCGT TATGGCATGG ACAACACCCA AAATGATACC 
AAATCGGTTG AATCATTTCC AGCAACTTTG AAGGTCCTCA GGAAATTATA TCCCCTCTTC 
GAGATCGTTA GCAATGAAAT GTATAGACTG ATGAATAACG ATTCCTTTCA AAAATTTACC 
CAGTCAGACG TATACAAAGA TGCTAGCGCT TTAATAGAAA TCCAAGAAAA GTGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XII.
Nature387(6632 Suppl)87-90(1997 May)
Johnston M,Hillier L,Riles L,Albermann K,Andr? B,Ansorge W,Benes V,Br?ckner M,Delius H,Dubois E,D?sterh?ft A,Entian KD,Floeth M,Goffeau A,Hebling U,Heumann K,Heuss-Neitzel D,Hilbert H,Hilger F,Kleine K,K?tter P,Louis EJ,Messenguy F,Mewes HW,Hoheisel JD

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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SST2 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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