SIR3 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	SIR3
Entrez Gene ID	851163
Full Name	chromatin-silencing protein SIR3
Synonyms	CMT1,MAR2,STE8
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001182330.3	NP_013547.3	chromatin-silencing protein SIR3

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XII.
Johnston M, Hillier L, Riles L, Albermann K, Andr? B, Ansorge W, Benes V, Br?ckner M, Delius H, Dubois E, D?sterh?ft A, Entian KD, Floeth M, Goffeau A, Hebling U, Heumann K, Heuss-Neitzel D, Hilbert H, Hilger F, Kleine K, K?tter P, Louis EJ, Messenguy F, Mewes HW, Hoheisel JD
Nature387(6632 Suppl)87-90(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Repair of UV-induced DNA lesions in Saccharomyces cerevisiae telomeres is moderated by Sir2 and Sir3, and inhibited by yKu-Sir4 interaction.
Title: Repair of UV-induced DNA lesions in natural Saccharomyces cerevisiae telomeres is moderated by Sir2 and Sir3, and inhibited by yKu-Sir4 interaction.

Here, the authors analyze binding of the Sir proteins to reconstituted mono-, di-, tri-, and tetra-nucleosomal chromatin templates and show that key Sir-Sir interactions bridge only sites on different nucleosomes but not sites on the same nucleosome, and are therefore 'interrupted' with respect to sites on the same nucleosome.
Title: Heterochromatin assembly by interrupted Sir3 bridges across neighboring nucleosomes.

silencing requires Sir2, a highly-conserved NAD(+)-dependent histone deacetylase. At locations other than the rDNA, silencing also requires additional Sir proteins, Sir1, Sir3, and Sir4 that together form a repressive heterochromatin-like structure termed silent chromatin.
Title: The Nuts and Bolts of Transcriptionally Silent Chromatin in Saccharomyces cerevisiae.

results suggest that Sir3 exhibits novel binding parameters and gene regulatory functions at the CN binding domains.
Title: Analysis of novel Sir3 binding regions in Saccharomyces cerevisiae.

Direct interactions promote eviction of the Sir3 heterochromatin protein by the SWI/SNF chromatin remodeling enzyme.
Title: Direct interactions promote eviction of the Sir3 heterochromatin protein by the SWI/SNF chromatin remodeling enzyme.

The following SIR3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SIR3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi00755	NM_001182330.3 Latest version!	Saccharomyces cerevisiae S288C chromatin-silencing protein SIR3 (SIR3), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SIR3 ( NM_001182330.3 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_013547.3 Saccharomyces cerevisiae S288C chromatin-silencing protein SIR3 (SIR3), partial mRNA.

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CloneID	OSi00755
Clone ID Related Accession (Same CDS sequence)	NM_001182330.3
Accession Version	NM_001182330.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2937bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	chromatin-silencing protein SIR3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001144). On Jul 30, 2012 this sequence version replaced NM_001182330.2. ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGGCTAAAA CATTGAAAGA TTTGGACGGT TGGCAAGTTA TCATTACAGA TGATCAGGGG 
AGGGTTATTG ACGACAACAA TAGAAGAAGA TCAAGAAAAA GAGGAGGAGA AAACGTATTT 
CTGAAAAGAA TTTCTGATGG ATTGAGTTTT GGGAAGGGTG AGAGCGTGAT ATTTAATGAT 
AATGTGACGG AAACATATTC TGTCTACTTG ATCCATGAGA TCAGACTCAA TACACTTAAT 
AATGTTGTGG AGATTTGGGT TTTTTCTTAC TTGAGATGGT TTGAACTCAA ACCAAAACTG 
TACTACGAAC AATTCAGGCC AGATCTAATA AAGGAAGATC ATCCTTTAGA ATTTTACAAA 
GATAAATTTT TCAACGAAGT AAATAAGAGC GAGTTATATT TAACTGCTGA GCTATCAGAG 
ATTTGGCTTA AGGATTTCAT TGCAGTTGGA CAGATACTGC CAGAGTCACA ATGGAATGAT 
AGTAGTATCG ATAAAATAGA GGATAGAGAT TTTCTAGTAC GTTATGCATG CGAACCTACT 
GCGGAAAAGT TTGTACCAAT TGATATTTTT CAAATCATAA GAAGAGTAAA GGAAATGGAA 
CCCAAACAAT CTGATGAATA CTTGAAAAGA GTATCTGTAC CAGTGAGTGG GCAGAAGACA 
AATAGACAGG TGATGCATAA GATGGGAGTT GAAAGATCTT CAAAAAGACT AGCAAAGAAA 
CCTTCAATGA AAAAAATTAA AATCGAGCCC TCTGCAGATG ATGACGTAAA TAATGGAAAT 
ATACCGTCTC AGCGAGGAAC GTCAACAACT CATGGTTCAA TTTCTCCCCA AGAAGAGTCT 
GTTTCCCCTA ACATCTCATC GGCGTCCCCT TCTGCGCTCA CATCACCTAC AGATTCTTCG 
AAAATTTTAC AAAAAAGATC TATATCAAAG GAACTAATTG TTTCAGAGGA AATACCAATA 
AACTCTTCAG AACAGGAATC CGATTATGAG CCAAACAATG AAACATCTGT GCTTTCAAGT 
AAACCTGGGT CAAAGCCAGA GAAGACATCT ACAGAACTCG TTGACGGCCG AGAGAATTTT 
GTATATGCAA ATAATCCAGA AGTGAGTGAT GATGGTGGGC TTGAAGAGGA AACAGATGAA 
GTTAGTTCAG AAAGTTCTGA TGAAGCAATA ATACCAGTAA ATAAAAGACG TGGCGCTCAC 
GGAAGCGAAT TGAGCAGTAA AATTAGAAAA ATCCACATTC AAGAAACACA AGAATTTTCT 
AAAAATTACA CTACAGAAAC AGATAACGAA ATGAACGGAA ATGGAAAACC TGGAATTCCC 
AGGGGCAACA CCAAAATTCA TAGTATGAAC GAAAATCCTA CACCAGAAAA AGGTAATGCA 
AAGATGATTG ATTTCGCTAC TTTGTCAAAG TTGAAAAAAA AATATCAGAT AATTTTAGAC 
CGGTTTGCAC CAGATAATCA AGTAACAGAT TCCTCCCAAT TGAACAAATT AACAGACGAA 
CAGTCAAGCT TGGACGTTGC AGGCCTTGAG GATAAGTTTA GAAAAGCATG CTCTTCATCT 
GGAAGAGAAA CCATTCTGTC AAATTTTAAC GCTGATATAA ATTTAGAGGA ATCAATTAGA 
GAATCTTTAC AAAAGAGAGA ACTACTAAAA TCACAAGTGG AGGATTTTAC AAGAATATTC 
CTTCCGATTT ACGACAGTTT GATGTCTTCC CAAAATAAAC TATTTTATAT TACCAATGCA 
GATGATTCTA CTAAATTCCA GCTTGTGAAT GATGTAATGG ATGAGTTGAT CACCTCTTCG 
GCCCGAAAGG AACTACCTAT TTTCGATTAC ATTCATATTG ATGCCTTGGA ACTGGCAGGA 
ATGGATGCAT TATATGAGAA AATATGGTTC GCCATTTCTA AAGAAAATCT ATGCGGTGAT 
ATATCACTAG AGGCCTTAAA CTTCTATATT ACAAATGTCC CGAAAGCAAA AAAGAGAAAG 
ACACTAATAT TAATACAAAA TCCGGAAAAT CTATTGAGTG AGAAGATTTT ACAATATTTC 
GAGAAATGGA TTTCTTCGAA AAATTCAAAA TTGTCTATCA TTTGCGTTGG CGGACATAAT 
GTGACGATTA GAGAGCAAAT AAATATAATG CCCTCCCTCA AAGCACACTT TACCGAAATC 
AAACTTAATA AGGTGGACAA GAATGAATTG CAACAAATGA TCATCACGCG ACTCAAATCT 
TTATTGAAAC CTTTTCATGT CAAAGTAAAT GATAAGAAGG AAATGACTAT TTACAATAAT 
ATTCGCGAAG GACAGAACCA GAAAATACCA GATAATGTGA TAGTAATCAA TCATAAGATC 
AACAACAAAA TCACTCAACT TATTGCAAAG AACGTAGCCA ACGTAAGTGG TAGTACTGAA 
AAAGCATTCA AAATATGTGA AGCTGCCGTA GAAATTTCTA AAAAAGACTT CGTACGGAAA 
GGTGGTCTAC AAAAGGGTAA ATTGGTAGTG TCACAGGAGA TGGTACCACG CTATTTCTCA 
GAAGCTATTA ACGGATTCAA GGATGAAACT ATTTCAAAGA AGATTATAGG AATGTCTCTG 
TTGATGAGAA CATTTTTATA TACCCTGGCG CAAGAAACCG AAGGCACGAA TCGTCATACG 
CTTGCTCTGG AGACCGTCCT GATTAAGATG GTGAAGATGT TGCGGGACAA CCCAGGGTAC 
AAGGCGTCAA AAGAAATTAA GAAGGTCATA TGCGGTGCCT GGGAGCCCGC AATAACTATA 
GAGAAACTAA AGCAATTTTC TTGGATAAGT GTAGTGAATG ATTTAGTGGG AGAAAAATTA 
GTTGTTGTGG TGCTCGAGGA GCCCAGTGCA AGCATTATGG TAGAACTAAA ACTTCCTTTA 
GAAATAAATT ACGCCTTTTC GATGGATGAA GAATTCAAAA ATATGGACTG CATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_013547.3
CDS	1..2937
Translation	MAKTLKDLDGWQVIITDDQGRVIDDNNRRRSRKRGGENVFLKRISDGLSFGKGESVIFNDNVTETYSVYLIHEIRLNTLNNVVEIWVFSYLRWFELKPKLYYEQFRPDLIKEDHPLEFYKDKFFNEVNKSELYLTAELSEIWLKDFIAVGQILPESQWNDSSIDKIEDRDFLVRYACEPTAEKFVPIDIFQIIRRVKEMEPKQSDEYLKRVSVPVSGQKTNRQVMHKMGVERSSKRLAKKPSMKKIKIEPSADDDVNNGNIPSQRGTSTTHGSISPQEESVSPNISSASPSALTSPTDSSKILQKRSISKELIVSEEIPINSSEQESDYEPNNETSVLSSKPGSKPEKTSTELVDGRENFVYANNPEVSDDGGLEEETDEVSSESSDEAIIPVNKRRGAHGSELSSKIRKIHIQETQEFSKNYTTETDNEMNGNGKPGIPRGNTKIHSMNENPTPEKGNAKMIDFATLSKLKKKYQIILDRFAPDNQVTDSSQLNKLTDEQSSLDVAGLEDKFRKACSSSGRETILSNFNADINLEESIRESLQKRELLKSQVEDFTRIFLPIYDSLMSSQNKLFYITNADDSTKFQLVNDVMDELITSSARKELPIFDYIHIDALELAGMDALYEKIWFAISKENLCGDISLEALNFYITNVPKAKKRKTLILIQNPENLLSEKILQYFEKWISSKNSKLSIICVGGHNVTIREQINIMPSLKAHFTEIKLNKVDKNELQQMIITRLKSLLKPFHVKVNDKKEMTIYNNIREGQNQKIPDNVIVINHKINNKITQLIAKNVANVSGSTEKAFKICEAAVEISKKDFVRKGGLQKGKLVVSQEMVPRYFSEAINGFKDETISKKIIGMSLLMRTFLYTLAQETEGTNRHTLALETVLIKMVKMLRDNPGYKASKEIKKVICGAWEPAITIEKLKQFSWISVVNDLVGEKLVVVVLEEPSASIMVELKLPLEINYAFSMDEEFKNMDCI

Target ORF information:

RefSeq Version	NM_001182330.3
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C chromatin-silencing protein SIR3 (SIR3), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001182330.3

ORF Insert Sequence:

ATGGCTAAAA CATTGAAAGA TTTGGACGGT TGGCAAGTTA TCATTACAGA TGATCAGGGG 
AGGGTTATTG ACGACAACAA TAGAAGAAGA TCAAGAAAAA GAGGAGGAGA AAACGTATTT 
CTGAAAAGAA TTTCTGATGG ATTGAGTTTT GGGAAGGGTG AGAGCGTGAT ATTTAATGAT 
AATGTGACGG AAACATATTC TGTCTACTTG ATCCATGAGA TCAGACTCAA TACACTTAAT 
AATGTTGTGG AGATTTGGGT TTTTTCTTAC TTGAGATGGT TTGAACTCAA ACCAAAACTG 
TACTACGAAC AATTCAGGCC AGATCTAATA AAGGAAGATC ATCCTTTAGA ATTTTACAAA 
GATAAATTTT TCAACGAAGT AAATAAGAGC GAGTTATATT TAACTGCTGA GCTATCAGAG 
ATTTGGCTTA AGGATTTCAT TGCAGTTGGA CAGATACTGC CAGAGTCACA ATGGAATGAT 
AGTAGTATCG ATAAAATAGA GGATAGAGAT TTTCTAGTAC GTTATGCATG CGAACCTACT 
GCGGAAAAGT TTGTACCAAT TGATATTTTT CAAATCATAA GAAGAGTAAA GGAAATGGAA 
CCCAAACAAT CTGATGAATA CTTGAAAAGA GTATCTGTAC CAGTGAGTGG GCAGAAGACA 
AATAGACAGG TGATGCATAA GATGGGAGTT GAAAGATCTT CAAAAAGACT AGCAAAGAAA 
CCTTCAATGA AAAAAATTAA AATCGAGCCC TCTGCAGATG ATGACGTAAA TAATGGAAAT 
ATACCGTCTC AGCGAGGAAC GTCAACAACT CATGGTTCAA TTTCTCCCCA AGAAGAGTCT 
GTTTCCCCTA ACATCTCATC GGCGTCCCCT TCTGCGCTCA CATCACCTAC AGATTCTTCG 
AAAATTTTAC AAAAAAGATC TATATCAAAG GAACTAATTG TTTCAGAGGA AATACCAATA 
AACTCTTCAG AACAGGAATC CGATTATGAG CCAAACAATG AAACATCTGT GCTTTCAAGT 
AAACCTGGGT CAAAGCCAGA GAAGACATCT ACAGAACTCG TTGACGGCCG AGAGAATTTT 
GTATATGCAA ATAATCCAGA AGTGAGTGAT GATGGTGGGC TTGAAGAGGA AACAGATGAA 
GTTAGTTCAG AAAGTTCTGA TGAAGCAATA ATACCAGTAA ATAAAAGACG TGGCGCTCAC 
GGAAGCGAAT TGAGCAGTAA AATTAGAAAA ATCCACATTC AAGAAACACA AGAATTTTCT 
AAAAATTACA CTACAGAAAC AGATAACGAA ATGAACGGAA ATGGAAAACC TGGAATTCCC 
AGGGGCAACA CCAAAATTCA TAGTATGAAC GAAAATCCTA CACCAGAAAA AGGTAATGCA 
AAGATGATTG ATTTCGCTAC TTTGTCAAAG TTGAAAAAAA AATATCAGAT AATTTTAGAC 
CGGTTTGCAC CAGATAATCA AGTAACAGAT TCCTCCCAAT TGAACAAATT AACAGACGAA 
CAGTCAAGCT TGGACGTTGC AGGCCTTGAG GATAAGTTTA GAAAAGCATG CTCTTCATCT 
GGAAGAGAAA CCATTCTGTC AAATTTTAAC GCTGATATAA ATTTAGAGGA ATCAATTAGA 
GAATCTTTAC AAAAGAGAGA ACTACTAAAA TCACAAGTGG AGGATTTTAC AAGAATATTC 
CTTCCGATTT ACGACAGTTT GATGTCTTCC CAAAATAAAC TATTTTATAT TACCAATGCA 
GATGATTCTA CTAAATTCCA GCTTGTGAAT GATGTAATGG ATGAGTTGAT CACCTCTTCG 
GCCCGAAAGG AACTACCTAT TTTCGATTAC ATTCATATTG ATGCCTTGGA ACTGGCAGGA 
ATGGATGCAT TATATGAGAA AATATGGTTC GCCATTTCTA AAGAAAATCT ATGCGGTGAT 
ATATCACTAG AGGCCTTAAA CTTCTATATT ACAAATGTCC CGAAAGCAAA AAAGAGAAAG 
ACACTAATAT TAATACAAAA TCCGGAAAAT CTATTGAGTG AGAAGATTTT ACAATATTTC 
GAGAAATGGA TTTCTTCGAA AAATTCAAAA TTGTCTATCA TTTGCGTTGG CGGACATAAT 
GTGACGATTA GAGAGCAAAT AAATATAATG CCCTCCCTCA AAGCACACTT TACCGAAATC 
AAACTTAATA AGGTGGACAA GAATGAATTG CAACAAATGA TCATCACGCG ACTCAAATCT 
TTATTGAAAC CTTTTCATGT CAAAGTAAAT GATAAGAAGG AAATGACTAT TTACAATAAT 
ATTCGCGAAG GACAGAACCA GAAAATACCA GATAATGTGA TAGTAATCAA TCATAAGATC 
AACAACAAAA TCACTCAACT TATTGCAAAG AACGTAGCCA ACGTAAGTGG TAGTACTGAA 
AAAGCATTCA AAATATGTGA AGCTGCCGTA GAAATTTCTA AAAAAGACTT CGTACGGAAA 
GGTGGTCTAC AAAAGGGTAA ATTGGTAGTG TCACAGGAGA TGGTACCACG CTATTTCTCA 
GAAGCTATTA ACGGATTCAA GGATGAAACT ATTTCAAAGA AGATTATAGG AATGTCTCTG 
TTGATGAGAA CATTTTTATA TACCCTGGCG CAAGAAACCG AAGGCACGAA TCGTCATACG 
CTTGCTCTGG AGACCGTCCT GATTAAGATG GTGAAGATGT TGCGGGACAA CCCAGGGTAC 
AAGGCGTCAA AAGAAATTAA GAAGGTCATA TGCGGTGCCT GGGAGCCCGC AATAACTATA 
GAGAAACTAA AGCAATTTTC TTGGATAAGT GTAGTGAATG ATTTAGTGGG AGAAAAATTA 
GTTGTTGTGG TGCTCGAGGA GCCCAGTGCA AGCATTATGG TAGAACTAAA ACTTCCTTTA 
GAAATAAATT ACGCCTTTTC GATGGATGAA GAATTCAAAA ATATGGACTG CATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XII.
Nature387(6632 Suppl)87-90(1997 May)
Johnston M,Hillier L,Riles L,Albermann K,Andr? B,Ansorge W,Benes V,Br?ckner M,Delius H,Dubois E,D?sterh?ft A,Entian KD,Floeth M,Goffeau A,Hebling U,Heumann K,Heuss-Neitzel D,Hilbert H,Hilger F,Kleine K,K?tter P,Louis EJ,Messenguy F,Mewes HW,Hoheisel JD

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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SIR3 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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