DCK1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	DCK1
Entrez Gene ID	851141
Full Name	Dck1p
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001182310.1	NP_013526.1	Dck1p

Pathways from BioSystems

Homologies

Eremothecium gossypii	AGOS_AER102W	NP_984962.1
Saccharomyces cerevisiae (baker's yeast)	YLR422W	NP_013526.1
Kluyveromyces lactis	KLLA0D02860g	XP_453195.1

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XII.
Johnston M, Hillier L, Riles L, Albermann K, Andr? B, Ansorge W, Benes V, Br?ckner M, Delius H, Dubois E, D?sterh?ft A, Entian KD, Floeth M, Goffeau A, Hebling U, Heumann K, Heuss-Neitzel D, Hilbert H, Hilger F, Kleine K, K?tter P, Louis EJ, Messenguy F, Mewes HW, Hoheisel JD
Nature387(6632 Suppl)87-90(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

A regulatory role in autophagy/mitophagy is suggested by the colocalization of Rho5 with autophagic markers and the decreased mitochondrial turnover observed in dck1, lmo1 and rho5 deletion mutants. Rho5 activation may thus serve as a central hub for the integration of different signaling pathways.
Title: Identification of Dck1 and Lmo1 as upstream regulators of the small GTPase Rho5 in Saccharomyces cerevisiae.

The following DCK1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DCK1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi00736	NM_001182310.1 Latest version!	Saccharomyces cerevisiae S288C Dck1p (DCK1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DCK1 ( NM_001182310.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_013526.1 Saccharomyces cerevisiae S288C Dck1p (DCK1), partial mRNA.

Price & Availability↑

CloneID	OSi00736
Clone ID Related Accession (Same CDS sequence)	NM_001182310.1
Accession Version	NM_001182310.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5799bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	Dck1p
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001144). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGTCGCAAC AGGATTCACA GCGCTGGCTG CCCACGGATC GGCTAATATA TGGTGTGCTA 
GTCAAATCTT TCCTTCCCTT ACAAAGGTAT CCTGAGCTGG TATATGAAAA CAGCAATTAT 
GCGAACGTTT ATGTGGGTGC AGAAGTTTAC GTCTTCGAAG AATCTGTGGA TAAGAAATGG 
TGTAGAGCGT ACCAATGTTT ACGACCCTTT CCTGAAGAAT TCATATCGAA TATGAACTCG 
GCGAATGATG TACTGCCAGA CGTGAAACCG AAAGTAGTGA TATTTCCTAG AAAATATGTT 
CATTTTGAAG CAGAAAAAGC CGTCAGCACA ATGCCATTTT TCAAAGCACC TAGTGCTGAG 
GATTTTAAAC CATTGATAAG TAAAGAATGT GAGTCCCGCT CCTTTTGTGA CAGCCTATAT 
GTTAGCTCTA CTGATGATAT ATCAACTGGT AAACCAAGAA AGACACCGAG ACCTCCGTTC 
CCCTTTTTCA GATACCAAAA GAGGTCCTTT AAGGACGAAA TGGGTCCTAT ATTATCTCTT 
ATTTCCTCGC ATGTTTACTC TATGTATTCA ATTGGCGAGT TTTCTATATA TAGGAAAATG 
ATAAAACTTT ATTATGATTT GGACACTATA CGATTTAGAT TGTCTATGAA TTTAACCACA 
GAGGCTGAGA AAATAAATCT GATAAGAGCA GCAACATCCC TAAGAACTAA AATCGCAAAA 
TTTTTGTCCT CCACATACAG GAAGAATAAA CTAATTGCTA ACTCTACTCC AAGAAACCCA 
GATCCTTATG GTTTCGAAGG AATTTTTGCC AGGGATATTG ATACTGGCGA ATTGCTCTCA 
TACGAGATCG ACAAATTAAG AACTTTAGTG TCATCGTCAA TGCTTTGTGG TTTAACTAAT 
AATTTTCCTA CTGTACCTGT GGTAGAGTCC GATGATGAGT CCTCATCTAA CGGACTATTC 
GGCACAGTTA GATCGAGCAT TTTGGTTAAC TTGAAAGATC TGGCATGGGA TCCCTCTATA 
TCAGATCCTA AATATCAAGA TCTTTCTATT TGTGTATACT TGAGAACCAA AGACGAAGTT 
TTAACAGAGT CTTTTACCAT GACGAAGTCG TCCAATATGG AATCAGCTCT AGATGAAATT 
CCTGCAATGT TATTCAAGAA TATTTTGGAA ACGATAGTCC ATAAAAATAA AGTATACTTA 
GTAGTAGTGC TGAAGGAAAC AATAGCAATT ACAACAGAAA CTGCTCCGGA AATTTCATCT 
TACAATATAT CTACCGAAGA GTCAAGTTCA CATTCCCCAT TTTCTCCATT TAATTCATCG 
ACTGAGAATA AGATTGACCA CGTAAAAAAG GGCTTAGCTG CCGGAGTTAT TAACATATCT 
CCGGTATTCA AGTTTTATAA CGGCCTATCA GTTGCGAATA AAGCTCAGAG GTTTAACCTT 
TATCTTTATA GCTCCGATTC CTCGGATTCG CAAAACTTCA ACTCTTCAAA GGATGCGGAT 
TTAGGTTGGG GCGGGTTAAT AAATAAGATA ATAAAAGATT CTTCTGAAGG CGTTTCTGTG 
AACCCTAGGG CTGTTTCTTT ATCGGTAACT GTAAAGGAAA TTATAGGCAA ACAAGAGGCG 
GAAAAAGTGC TCTCCACATC TTTAGTACCG ATAAGATCTA TTCCCACATA CTTTTATGAT 
ACCATGTTTA GCCAAGCAGA AAGAATTTAT CTGAACTTAG GTAGGGTGTC GCTTTATGGA 
CTTCCTGCAG CTGATACAAA TATAGAAAAT GTTACCGTTC AAATATCCTG CAGGAATAAG 
GCCGTAAAAT TTTGCAAAAA TAAGCTCGAA GAGAGATCTG GGGACTGGAA GTTTGTTTCA 
GTTCGTCCAA ATGAGAGCAT AGGCGAATCG ATTAGAATAG AAGGGGTAGA GAACATGAAC 
GAAGACGAGA CATTAAGAGT ATTGGTATAT TTGAATGGAT TTTTGATGGC AAAATCAAAT 
ATACATATCA AGAAAAAGAA CGAAATCATA GAATATAGAA AGGGTACCGT ATTCCAAATA 
ATGTCATCCA AATCAGTACC TCTAATTCAT TTAGAGCTTG AAGCATCATA TTTTGGTCGG 
AGATATAATA TCAACCCTGC CATTACAAAT TTCTTAGTTC TTCAAACCAA AAATGTTGAA 
TTCGACCAGC AACTTAAAGA GCACTATTCA GTTACATTGA AGCAGCTGAA TAACGTTTCT 
TTTAAAGATT TACTAAAGCA TTTTGACACA ATTTTAGCAC ACTATTTGTT ACTTCTTGAA 
TCGGTGAATG AAGCTACTGA TAAAAAAGGC CCTTCTTCAA GTTTACCCAA TATTGTATTC 
AGTGAATTCG TCAAGTTTTT AAATTTAATG TTAACACACC AAGAAAACTC TAGATATTGG 
TTTAATAGAC TGTACAAAAA AGTCATGTCA AAGGAACTAG AATGTCCCAA TGTTGCACCG 
ATACTAATAA AACATATGAC AACGATCTTT GATAGGAGTC ATTCTTCTTG GACTAGAACA 
GGAACTGCAA TCTGCCGAAC CATCCTGTAC ATAATTGTGT TGGCAATCGG TTCGTCTCAT 
TCTGATGAAA TGCCTAATTT CAGTCATTTT TTCAGAAGCT TGCACAAATT TTTAATGCTT 
GCTGATGAGC CAATAATGGC GGATCAAATT TTGTTAATAG AAAGTATTCC CTCTATGCTG 
GAGACAATGA CCAATCACTG TAAGGTGGAA GACTTAGTTC GTTTTGCGAT CGGACTGTTT 
GAATGTTGCC AGGAAAAAGA AATGAATCAA AAAATGTACT CCAGGCCACT CTCAGTTCGA 
GAAGAAGAAT ATTTGAATAC AAAGTTTAAT TGCCTGTTGA AGTTAATTAA CAAGAAGGTT 
TTGCAAAATT ATTTAACCAA TACGGAATCC GTAGATAAAC TGAGGTTACA GTTTTTATCT 
AAAACCCTTG AGTGGTTACT TACGCCGTAT ACTCCAGGCG ATGACAAATG TTTTCACGTA 
GAATCCTTAC GCTTGGTTAA TTCTGTTTTC ATTACAATAA TCGAAGATTA CAAATTTGAC 
ATGCTACAAA GGAACTTGAT AAGGTTACTA CCATATTTGT GTAAGTCCTT CGTCCATTTA 
AGAAGGTACT GTAAGAAAGC ACGTTTAATG AGGCCTCGAA GAGTTTTTAC CATGCTTTTT 
CCCAGAGAAA TTCCATGCAA CTACATACCG GTTGATTCAA TAGTGAATGA TGAAGTAGTC 
GTAGAAGTTC TTCTAGAGCT TGCCATCATA ATTTGTGAAA TAACGAAAAT TGCATCAAGC 
AGATTTCCAT CATATCAATC TTTCAGCGAA ATCATAAACT TGTGCGATAA AGACACTCTT 
TTTCAATCAA ATTTTTATTC ACGACAAATC ACGAATGAAA ATGTCTATAC AATAACCAAA 
ACAGTTTTTT TGTTTTTTAA ACAAGATTGG TTCCCAGGAA TGAAGTGGCT TGGCGTATCA 
GCGTTATTAG GAAGATCGTC TCTAATATTG CTTAGTTTAT GCAAGGATTA TATCATAGAA 
AATAATTCAC CTTCTCCATC AAAGGAGTCT GAAAAGAGGG TTGACATGCG ACTTTGGGCA 
GAATATGTAA AAGTCATACT ACTAGTTTCA AACCATAAAA GTGCATCCTT GACTAAGTTA 
GCTATTACTC CACGTAAGGC CGTTTATTTG ATATCGGGTG ACCTGAAAAA AATTTCAGCA 
TATATCCTAA ATGAATGTTG GGATGCCCTA GCCACTGGGC ACTATAATAT CACTTATGCT 
AAAAAGTATG GATTAGGAGC CTTGAGTGAT TGCCAATTTG AATTGTTTGT TCATAATCAA 
TTTTTGATAC GTGAGATCTT TATTTTTGCT TTCCATAGAC ATATCGATGC AACAAGAATT 
TGTTGCAAAA TACTATGGGG ATTAGGGCTA AATTTTTGGA GGATATTTGG GTCCCTACAA 
CCAGCAGTGA ACGCATGTAT CCCAGAATTA TTCAGCGCAT ACCAAATCGG GAAGCTTCGT 
TTGAATGATT ACGAATTGGA AAGGTTTGTT TCTTGTTTGT TTTTTATGAT GCACGTTCCC 
GATTCTGACA CCTTTTTTCC TGCTTGTATG GATTTCTTAA GAGATTTACT TGGTTTTTTG 
CATATTGTAA ATGAAATCTA CAAAATACCT AATCAAGAAG AATTTGATGA TGATCGGACT 
GCTCGGCACA TAGAGATGTT CGAGTACTTG CTAGAAGCAA ACAGGCCAGA GTTGTTTCAT 
AAAATGATAT ATGATTTGTT TATCCACTTT ATTCAGAAAA AAGACTTTGT TCAAGCAGCT 
TTGAGTCTAG AACTCTTGGC AGGTACGTAC GCTTGGGATT CTAATGATAC TCTTGAAGCA 
ATATCTTTCC CGCCATTGCC AGAGCAATCT TCATTTGAGA GGAAGGAATA TCTTCTTAAG 
GAATCCGCAA GAAACTTTTC GAGAGGCCAA AAACCAGAAA AAGCTTTAGC GGTATACAAG 
GACTTGATAA AAGCCTACGA TGAGATCAAT TATGATTTAA ACGGGCTGGC CTTTGTACAT 
GACCAAATAG CAGGCATTTA TACCAGATTA CAGTCCATTG ATAGACTGGT TCCAACGTAT 
TTTAAAGTTT CTTTCATGGG ATTTGGTTTT CCAAAATCAT TAAGAAATAA ATCGTTCGTC 
TTTGAGGGTC TCCCATTTGA ACACATTACG TCTATGCATG ACAGGCTTCT ACGAAGCTAT 
CATGGGTCAA ATATTGTACA CTCACAAGAA GAAGTGGATA TGTTATTGAT GAATCCTCCA 
ATGGGAAAAT ACATTCATGT AGCTTCCGTC GAACCGTGCT TAAGTATATC CGATAATTAC 
AACAGTAGTG ACAAGAAAAG CTCAATTAAC AACAAAGTGC GGATGTACAT TGAGAATAGA 
GATTTGAGGA CTTTCAGTAA TTCTCGACGT TTGCCAGGCG CAAAAGGAGT CACTGACCTG 
TGGGTGGAGG AATATACGTA TCATACTATG AATACTTTTC CAACACTAAT GAATAGATCA 
GAAATTGTAA AGGTTACGAA GAGCAAACTT TCTCCACTTG AAAATGCAAT TCGTTCCTTA 
CAAGTTAAAA TACAGGAGTT ATATGGGCTT GAAAACATGT GCAATAAAAC TCTGAAGGAC 
CACGGTGATG TGAACGATTT ATTTACCGAG CTTTCTACAA ATATTACGGG AACAATATCA 
GCTCCAGTTA ATGGAGGCAT ATCACAGTAC AAGGCCTTTT TAGAACCCTC CACATCCAAG 
CAGTTCAGTA CTGATGATTT AGGAAGATTG ACGCTAGCTT TTGATGAGCT TGTTGCAGTA 
TTAGGTCGCT GTCTAACGTT GCATGCAGAA CTACTACCAT CAAAGGATCT AAAACCATCA 
CATGATCTTC TAGTGCGTCT TTTTGAGGAA AATTTCGCTG AGGAGATAGA AAGATATAGT 
CGTACTCTCA GTGAGGCCAA TCGTAGCAGG AATAACATGA TTACTGCTCG AATAATTTCG 
CACAAGAACC CCAATAAAAA GGCAAGCTTT TCTGGAAGGG ATCATCACAC CTCAGGGTCC 
AATCATAGCC AATTTGTATT AGAACATAGC GACTCATTTG GCCCAAACAG TTTACTTTTT 
GGAAAATATT TAACAAGAAC GTTATCCCAC TCGTCCACTA CGAGCAGTTT GGATAAAAGT 
GGAATTGTTT CCGGCACTTC GAGCACCTTT TTGGCAGGGT CACAGCCAAA CACCAACACA 
GATTCACAGC ACAAGCATGA TTACTCACAT TCGGGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_013526.1
CDS	1..5799
Translation	MSQQDSQRWLPTDRLIYGVLVKSFLPLQRYPELVYENSNYANVYVGAEVYVFEESVDKKWCRAYQCLRPFPEEFISNMNSANDVLPDVKPKVVIFPRKYVHFEAEKAVSTMPFFKAPSAEDFKPLISKECESRSFCDSLYVSSTDDISTGKPRKTPRPPFPFFRYQKRSFKDEMGPILSLISSHVYSMYSIGEFSIYRKMIKLYYDLDTIRFRLSMNLTTEAEKINLIRAATSLRTKIAKFLSSTYRKNKLIANSTPRNPDPYGFEGIFARDIDTGELLSYEIDKLRTLVSSSMLCGLTNNFPTVPVVESDDESSSNGLFGTVRSSILVNLKDLAWDPSISDPKYQDLSICVYLRTKDEVLTESFTMTKSSNMESALDEIPAMLFKNILETIVHKNKVYLVVVLKETIAITTETAPEISSYNISTEESSSHSPFSPFNSSTENKIDHVKKGLAAGVINISPVFKFYNGLSVANKAQRFNLYLYSSDSSDSQNFNSSKDADLGWGGLINKIIKDSSEGVSVNPRAVSLSVTVKEIIGKQEAEKVLSTSLVPIRSIPTYFYDTMFSQAERIYLNLGRVSLYGLPAADTNIENVTVQISCRNKAVKFCKNKLEERSGDWKFVSVRPNESIGESIRIEGVENMNEDETLRVLVYLNGFLMAKSNIHIKKKNEIIEYRKGTVFQIMSSKSVPLIHLELEASYFGRRYNINPAITNFLVLQTKNVEFDQQLKEHYSVTLKQLNNVSFKDLLKHFDTILAHYLLLLESVNEATDKKGPSSSLPNIVFSEFVKFLNLMLTHQENSRYWFNRLYKKVMSKELECPNVAPILIKHMTTIFDRSHSSWTRTGTAICRTILYIIVLAIGSSHSDEMPNFSHFFRSLHKFLMLADEPIMADQILLIESIPSMLETMTNHCKVEDLVRFAIGLFECCQEKEMNQKMYSRPLSVREEEYLNTKFNCLLKLINKKVLQNYLTNTESVDKLRLQFLSKTLEWLLTPYTPGDDKCFHVESLRLVNSVFITIIEDYKFDMLQRNLIRLLPYLCKSFVHLRRYCKKARLMRPRRVFTMLFPREIPCNYIPVDSIVNDEVVVEVLLELAIIICEITKIASSRFPSYQSFSEIINLCDKDTLFQSNFYSRQITNENVYTITKTVFLFFKQDWFPGMKWLGVSALLGRSSLILLSLCKDYIIENNSPSPSKESEKRVDMRLWAEYVKVILLVSNHKSASLTKLAITPRKAVYLISGDLKKISAYILNECWDALATGHYNITYAKKYGLGALSDCQFELFVHNQFLIREIFIFAFHRHIDATRICCKILWGLGLNFWRIFGSLQPAVNACIPELFSAYQIGKLRLNDYELERFVSCLFFMMHVPDSDTFFPACMDFLRDLLGFLHIVNEIYKIPNQEEFDDDRTARHIEMFEYLLEANRPELFHKMIYDLFIHFIQKKDFVQAALSLELLAGTYAWDSNDTLEAISFPPLPEQSSFERKEYLLKESARNFSRGQKPEKALAVYKDLIKAYDEINYDLNGLAFVHDQIAGIYTRLQSIDRLVPTYFKVSFMGFGFPKSLRNKSFVFEGLPFEHITSMHDRLLRSYHGSNIVHSQEEVDMLLMNPPMGKYIHVASVEPCLSISDNYNSSDKKSSINNKVRMYIENRDLRTFSNSRRLPGAKGVTDLWVEEYTYHTMNTFPTLMNRSEIVKVTKSKLSPLENAIRSLQVKIQELYGLENMCNKTLKDHGDVNDLFTELSTNITGTISAPVNGGISQYKAFLEPSTSKQFSTDDLGRLTLAFDELVAVLGRCLTLHAELLPSKDLKPSHDLLVRLFEENFAEEIERYSRTLSEANRSRNNMITARIISHKNPNKKASFSGRDHHTSGSNHSQFVLEHSDSFGPNSLLFGKYLTRTLSHSSTTSSLDKSGIVSGTSSTFLAGSQPNTNTDSQHKHDYSHSG

Target ORF information:

RefSeq Version	NM_001182310.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C Dck1p (DCK1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001182310.1

ORF Insert Sequence:

ATGTCGCAAC AGGATTCACA GCGCTGGCTG CCCACGGATC GGCTAATATA TGGTGTGCTA 
GTCAAATCTT TCCTTCCCTT ACAAAGGTAT CCTGAGCTGG TATATGAAAA CAGCAATTAT 
GCGAACGTTT ATGTGGGTGC AGAAGTTTAC GTCTTCGAAG AATCTGTGGA TAAGAAATGG 
TGTAGAGCGT ACCAATGTTT ACGACCCTTT CCTGAAGAAT TCATATCGAA TATGAACTCG 
GCGAATGATG TACTGCCAGA CGTGAAACCG AAAGTAGTGA TATTTCCTAG AAAATATGTT 
CATTTTGAAG CAGAAAAAGC CGTCAGCACA ATGCCATTTT TCAAAGCACC TAGTGCTGAG 
GATTTTAAAC CATTGATAAG TAAAGAATGT GAGTCCCGCT CCTTTTGTGA CAGCCTATAT 
GTTAGCTCTA CTGATGATAT ATCAACTGGT AAACCAAGAA AGACACCGAG ACCTCCGTTC 
CCCTTTTTCA GATACCAAAA GAGGTCCTTT AAGGACGAAA TGGGTCCTAT ATTATCTCTT 
ATTTCCTCGC ATGTTTACTC TATGTATTCA ATTGGCGAGT TTTCTATATA TAGGAAAATG 
ATAAAACTTT ATTATGATTT GGACACTATA CGATTTAGAT TGTCTATGAA TTTAACCACA 
GAGGCTGAGA AAATAAATCT GATAAGAGCA GCAACATCCC TAAGAACTAA AATCGCAAAA 
TTTTTGTCCT CCACATACAG GAAGAATAAA CTAATTGCTA ACTCTACTCC AAGAAACCCA 
GATCCTTATG GTTTCGAAGG AATTTTTGCC AGGGATATTG ATACTGGCGA ATTGCTCTCA 
TACGAGATCG ACAAATTAAG AACTTTAGTG TCATCGTCAA TGCTTTGTGG TTTAACTAAT 
AATTTTCCTA CTGTACCTGT GGTAGAGTCC GATGATGAGT CCTCATCTAA CGGACTATTC 
GGCACAGTTA GATCGAGCAT TTTGGTTAAC TTGAAAGATC TGGCATGGGA TCCCTCTATA 
TCAGATCCTA AATATCAAGA TCTTTCTATT TGTGTATACT TGAGAACCAA AGACGAAGTT 
TTAACAGAGT CTTTTACCAT GACGAAGTCG TCCAATATGG AATCAGCTCT AGATGAAATT 
CCTGCAATGT TATTCAAGAA TATTTTGGAA ACGATAGTCC ATAAAAATAA AGTATACTTA 
GTAGTAGTGC TGAAGGAAAC AATAGCAATT ACAACAGAAA CTGCTCCGGA AATTTCATCT 
TACAATATAT CTACCGAAGA GTCAAGTTCA CATTCCCCAT TTTCTCCATT TAATTCATCG 
ACTGAGAATA AGATTGACCA CGTAAAAAAG GGCTTAGCTG CCGGAGTTAT TAACATATCT 
CCGGTATTCA AGTTTTATAA CGGCCTATCA GTTGCGAATA AAGCTCAGAG GTTTAACCTT 
TATCTTTATA GCTCCGATTC CTCGGATTCG CAAAACTTCA ACTCTTCAAA GGATGCGGAT 
TTAGGTTGGG GCGGGTTAAT AAATAAGATA ATAAAAGATT CTTCTGAAGG CGTTTCTGTG 
AACCCTAGGG CTGTTTCTTT ATCGGTAACT GTAAAGGAAA TTATAGGCAA ACAAGAGGCG 
GAAAAAGTGC TCTCCACATC TTTAGTACCG ATAAGATCTA TTCCCACATA CTTTTATGAT 
ACCATGTTTA GCCAAGCAGA AAGAATTTAT CTGAACTTAG GTAGGGTGTC GCTTTATGGA 
CTTCCTGCAG CTGATACAAA TATAGAAAAT GTTACCGTTC AAATATCCTG CAGGAATAAG 
GCCGTAAAAT TTTGCAAAAA TAAGCTCGAA GAGAGATCTG GGGACTGGAA GTTTGTTTCA 
GTTCGTCCAA ATGAGAGCAT AGGCGAATCG ATTAGAATAG AAGGGGTAGA GAACATGAAC 
GAAGACGAGA CATTAAGAGT ATTGGTATAT TTGAATGGAT TTTTGATGGC AAAATCAAAT 
ATACATATCA AGAAAAAGAA CGAAATCATA GAATATAGAA AGGGTACCGT ATTCCAAATA 
ATGTCATCCA AATCAGTACC TCTAATTCAT TTAGAGCTTG AAGCATCATA TTTTGGTCGG 
AGATATAATA TCAACCCTGC CATTACAAAT TTCTTAGTTC TTCAAACCAA AAATGTTGAA 
TTCGACCAGC AACTTAAAGA GCACTATTCA GTTACATTGA AGCAGCTGAA TAACGTTTCT 
TTTAAAGATT TACTAAAGCA TTTTGACACA ATTTTAGCAC ACTATTTGTT ACTTCTTGAA 
TCGGTGAATG AAGCTACTGA TAAAAAAGGC CCTTCTTCAA GTTTACCCAA TATTGTATTC 
AGTGAATTCG TCAAGTTTTT AAATTTAATG TTAACACACC AAGAAAACTC TAGATATTGG 
TTTAATAGAC TGTACAAAAA AGTCATGTCA AAGGAACTAG AATGTCCCAA TGTTGCACCG 
ATACTAATAA AACATATGAC AACGATCTTT GATAGGAGTC ATTCTTCTTG GACTAGAACA 
GGAACTGCAA TCTGCCGAAC CATCCTGTAC ATAATTGTGT TGGCAATCGG TTCGTCTCAT 
TCTGATGAAA TGCCTAATTT CAGTCATTTT TTCAGAAGCT TGCACAAATT TTTAATGCTT 
GCTGATGAGC CAATAATGGC GGATCAAATT TTGTTAATAG AAAGTATTCC CTCTATGCTG 
GAGACAATGA CCAATCACTG TAAGGTGGAA GACTTAGTTC GTTTTGCGAT CGGACTGTTT 
GAATGTTGCC AGGAAAAAGA AATGAATCAA AAAATGTACT CCAGGCCACT CTCAGTTCGA 
GAAGAAGAAT ATTTGAATAC AAAGTTTAAT TGCCTGTTGA AGTTAATTAA CAAGAAGGTT 
TTGCAAAATT ATTTAACCAA TACGGAATCC GTAGATAAAC TGAGGTTACA GTTTTTATCT 
AAAACCCTTG AGTGGTTACT TACGCCGTAT ACTCCAGGCG ATGACAAATG TTTTCACGTA 
GAATCCTTAC GCTTGGTTAA TTCTGTTTTC ATTACAATAA TCGAAGATTA CAAATTTGAC 
ATGCTACAAA GGAACTTGAT AAGGTTACTA CCATATTTGT GTAAGTCCTT CGTCCATTTA 
AGAAGGTACT GTAAGAAAGC ACGTTTAATG AGGCCTCGAA GAGTTTTTAC CATGCTTTTT 
CCCAGAGAAA TTCCATGCAA CTACATACCG GTTGATTCAA TAGTGAATGA TGAAGTAGTC 
GTAGAAGTTC TTCTAGAGCT TGCCATCATA ATTTGTGAAA TAACGAAAAT TGCATCAAGC 
AGATTTCCAT CATATCAATC TTTCAGCGAA ATCATAAACT TGTGCGATAA AGACACTCTT 
TTTCAATCAA ATTTTTATTC ACGACAAATC ACGAATGAAA ATGTCTATAC AATAACCAAA 
ACAGTTTTTT TGTTTTTTAA ACAAGATTGG TTCCCAGGAA TGAAGTGGCT TGGCGTATCA 
GCGTTATTAG GAAGATCGTC TCTAATATTG CTTAGTTTAT GCAAGGATTA TATCATAGAA 
AATAATTCAC CTTCTCCATC AAAGGAGTCT GAAAAGAGGG TTGACATGCG ACTTTGGGCA 
GAATATGTAA AAGTCATACT ACTAGTTTCA AACCATAAAA GTGCATCCTT GACTAAGTTA 
GCTATTACTC CACGTAAGGC CGTTTATTTG ATATCGGGTG ACCTGAAAAA AATTTCAGCA 
TATATCCTAA ATGAATGTTG GGATGCCCTA GCCACTGGGC ACTATAATAT CACTTATGCT 
AAAAAGTATG GATTAGGAGC CTTGAGTGAT TGCCAATTTG AATTGTTTGT TCATAATCAA 
TTTTTGATAC GTGAGATCTT TATTTTTGCT TTCCATAGAC ATATCGATGC AACAAGAATT 
TGTTGCAAAA TACTATGGGG ATTAGGGCTA AATTTTTGGA GGATATTTGG GTCCCTACAA 
CCAGCAGTGA ACGCATGTAT CCCAGAATTA TTCAGCGCAT ACCAAATCGG GAAGCTTCGT 
TTGAATGATT ACGAATTGGA AAGGTTTGTT TCTTGTTTGT TTTTTATGAT GCACGTTCCC 
GATTCTGACA CCTTTTTTCC TGCTTGTATG GATTTCTTAA GAGATTTACT TGGTTTTTTG 
CATATTGTAA ATGAAATCTA CAAAATACCT AATCAAGAAG AATTTGATGA TGATCGGACT 
GCTCGGCACA TAGAGATGTT CGAGTACTTG CTAGAAGCAA ACAGGCCAGA GTTGTTTCAT 
AAAATGATAT ATGATTTGTT TATCCACTTT ATTCAGAAAA AAGACTTTGT TCAAGCAGCT 
TTGAGTCTAG AACTCTTGGC AGGTACGTAC GCTTGGGATT CTAATGATAC TCTTGAAGCA 
ATATCTTTCC CGCCATTGCC AGAGCAATCT TCATTTGAGA GGAAGGAATA TCTTCTTAAG 
GAATCCGCAA GAAACTTTTC GAGAGGCCAA AAACCAGAAA AAGCTTTAGC GGTATACAAG 
GACTTGATAA AAGCCTACGA TGAGATCAAT TATGATTTAA ACGGGCTGGC CTTTGTACAT 
GACCAAATAG CAGGCATTTA TACCAGATTA CAGTCCATTG ATAGACTGGT TCCAACGTAT 
TTTAAAGTTT CTTTCATGGG ATTTGGTTTT CCAAAATCAT TAAGAAATAA ATCGTTCGTC 
TTTGAGGGTC TCCCATTTGA ACACATTACG TCTATGCATG ACAGGCTTCT ACGAAGCTAT 
CATGGGTCAA ATATTGTACA CTCACAAGAA GAAGTGGATA TGTTATTGAT GAATCCTCCA 
ATGGGAAAAT ACATTCATGT AGCTTCCGTC GAACCGTGCT TAAGTATATC CGATAATTAC 
AACAGTAGTG ACAAGAAAAG CTCAATTAAC AACAAAGTGC GGATGTACAT TGAGAATAGA 
GATTTGAGGA CTTTCAGTAA TTCTCGACGT TTGCCAGGCG CAAAAGGAGT CACTGACCTG 
TGGGTGGAGG AATATACGTA TCATACTATG AATACTTTTC CAACACTAAT GAATAGATCA 
GAAATTGTAA AGGTTACGAA GAGCAAACTT TCTCCACTTG AAAATGCAAT TCGTTCCTTA 
CAAGTTAAAA TACAGGAGTT ATATGGGCTT GAAAACATGT GCAATAAAAC TCTGAAGGAC 
CACGGTGATG TGAACGATTT ATTTACCGAG CTTTCTACAA ATATTACGGG AACAATATCA 
GCTCCAGTTA ATGGAGGCAT ATCACAGTAC AAGGCCTTTT TAGAACCCTC CACATCCAAG 
CAGTTCAGTA CTGATGATTT AGGAAGATTG ACGCTAGCTT TTGATGAGCT TGTTGCAGTA 
TTAGGTCGCT GTCTAACGTT GCATGCAGAA CTACTACCAT CAAAGGATCT AAAACCATCA 
CATGATCTTC TAGTGCGTCT TTTTGAGGAA AATTTCGCTG AGGAGATAGA AAGATATAGT 
CGTACTCTCA GTGAGGCCAA TCGTAGCAGG AATAACATGA TTACTGCTCG AATAATTTCG 
CACAAGAACC CCAATAAAAA GGCAAGCTTT TCTGGAAGGG ATCATCACAC CTCAGGGTCC 
AATCATAGCC AATTTGTATT AGAACATAGC GACTCATTTG GCCCAAACAG TTTACTTTTT 
GGAAAATATT TAACAAGAAC GTTATCCCAC TCGTCCACTA CGAGCAGTTT GGATAAAAGT 
GGAATTGTTT CCGGCACTTC GAGCACCTTT TTGGCAGGGT CACAGCCAAA CACCAACACA 
GATTCACAGC ACAAGCATGA TTACTCACAT TCGGGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XII.
Nature387(6632 Suppl)87-90(1997 May)
Johnston M,Hillier L,Riles L,Albermann K,Andr? B,Ansorge W,Benes V,Br?ckner M,Delius H,Dubois E,D?sterh?ft A,Entian KD,Floeth M,Goffeau A,Hebling U,Heumann K,Heuss-Neitzel D,Hilbert H,Hilger F,Kleine K,K?tter P,Louis EJ,Messenguy F,Mewes HW,Hoheisel JD

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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DCK1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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