DCR2 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	DCR2
Entrez Gene ID	851075
Full Name	phosphoprotein phosphatase
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001182250.1	NP_013465.1	phosphoprotein phosphatase

Pathways from BioSystems

Homologies

Arabidopsis thaliana (thale cress)	PAP29	NP_201119.1
Kluyveromyces lactis	KLLA0B13134g	XP_452116.1
Saccharomyces cerevisiae (baker's yeast)	DCR2	NP_013465.1
Oryza sativa (rice)	Os09g0533300	NP_001063768.1

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XII.
Johnston M, Hillier L, Riles L, Albermann K, Andr? B, Ansorge W, Benes V, Br?ckner M, Delius H, Dubois E, D?sterh?ft A, Entian KD, Floeth M, Goffeau A, Hebling U, Heumann K, Heuss-Neitzel D, Hilbert H, Hilger F, Kleine K, K?tter P, Louis EJ, Messenguy F, Mewes HW, Hoheisel JD
Nature387(6632 Suppl)87-90(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

the Dcr2p phosphatase functionally and physically interacts with Sic1p. Over-expression of Dcr2p destabilizes Sic1p and leads to phenotypes associated with destabilized Sic1p, such as genome instability
Title: The Dcr2p phosphatase destabilizes Sic1p in Saccharomyces cerevisiae.

Overexpression of DCR2, but not of a catalytically inactive DCR2 allele, significantly delays HAC1 splicing and sensitizes cells to the UPR. Furthermore, Dcr2 physically interacts in vivo with Ire1-S840E,S841E, and de-phosphorylates Ire1 in vitro.
Title: Dcr2 targets Ire1 and downregulates the unfolded protein response in Saccharomyces cerevisiae.

Hym1p requires Dcr2p(a phosphatase)and Gid8p (involved in protein degradation) to promote the transition from the G1 to S phase of the cell cycle
Title: Roles of the RAM signaling network in cell cycle progression in Saccharomyces cerevisiae.

Gid8p (Dcr1p) and Dcr2p have roles in a common pathway to promote START completion in Saccharomyces cerevisiae
Title: Gid8p (Dcr1p) and Dcr2p function in a common pathway to promote START completion in Saccharomyces cerevisiae.

The following DCR2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DCR2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi00679	NM_001182250.1 Latest version!	Saccharomyces cerevisiae S288C phosphoprotein phosphatase (DCR2), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DCR2 ( NM_001182250.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_013465.1 Saccharomyces cerevisiae S288C phosphoprotein phosphatase (DCR2), partial mRNA.

Price & Availability↑

CloneID	OSi00679
Clone ID Related Accession (Same CDS sequence)	NM_001182250.1
Accession Version	NM_001182250.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1737bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	phosphoprotein phosphatase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001144). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGATCCGGT TACCGAGGCT GTATCAAAGG TACCTATTAT ACTTGGTAGT TTTTGTAGTG 
ATTGCCCTAT TTTACTTCCT ACAGGCGCCA AGAGTTGAAG AGCACATTGG TTTCGACCTT 
GCACTCCCGA TATCACACGT TGATAATCTA TGGTTCCAAA ATAAAGGTCT AGAGGGATTC 
TCAAATGACG ATAAGCTAGT TGTAAATATA GGCTATGACG AATGTTTTCA TATTGGTCGT 
TTTTATGAAG GATGTTTTAA TCGGCATGAA CTTAAATCAA CTTTAACTGA TGGGCACCAA 
TACTTACAAA GGAAAAGAAT TCATAAAGAC TTGAGAGGTT CCTTTGGACG CAGGTGGTTT 
GGAAAGTCAG AATACCTTTA CTATGATGTG TTATACCCTG CTTTGGTGGA CTACTTTGGA 
TCCAACCTAG AAAAGCTAAA TGTTGAGGCA GTAACTGGAA TTTCCAAATA TCCTAAAGAC 
AAATCGCTAC CCTTTATGGA TGTATCCATC ACATTCGAGC CTATTAGCAT AGAACTATTG 
CAGAAAAGGA GCTACATAAG CGATATTAAC ATTCTCTTTG GTGTTGATTG CATCCAGCCG 
ATAGCAAATT GGACTCTTCA AAAAGAATTT CCCTTAGTAA AATACCGCTA CAGCGAACCT 
GCTTATTTGA CTTACAAATT CGTTGGTACT CGTCCTGTAG ACACAGGAGC TCAACGGCTG 
CAAGAAACTG ATGAAGGAAA GTTCAAAATT GTTCAGTTGG CGGATCTCCA CTTGGGTGTG 
GGGGAGTCTG AATGTATCGA TGAGTATCCA AAGCATGAAG CTTGCAAGGC AGATCCAAAA 
ACGGAAACTT TCGTTCAACA AGTTCTTGAC ATTGAAAAAC CGCAGTTGGT TGTTTTTACT 
GGTGACCAAA TCATGGGTGA TAGATCTATT CAAGACTCTG AAACCGTATT ATTAAAGGCG 
GTAGCACCCG TCATTGCAAG AAAAATTCCG TGGGCAATGG TATGGGGAAA TCACGACGAC 
GAGGGAAGCT TAACGCGCTG GCAGTTATCC GAAATAGCTT CAGTACTACC ATATTCCCTT 
TTCAAGTTTA GTCCCCATGA TACACACGAT AATACGTTTG GCGTTGGTAA TTATATTTAT 
CAGATTTTTT CGAACAATGA TACAGAAGTC CCAGTAGGTA CACTTTATTT TCTGGACTCA 
CATAAATATT CTACGGTTGG TAAAATTTAT CCTGGATATG ATTGGATTAA GGAATCACAG 
TGGAAATACA TTGAAGATTA TCATGATGTG AACTTGAAGT TCAAAACAGG TCTTTCAATG 
GCATTTTTTC ACATCCCACT GCCAGAATAC TTGAATATTG AATCCAAGAC ACATCCCGGA 
GAGAAAAATC CCCTCATTGG AATGTACAAA GAAGGTGTTA CTGCACCTAA GTATAATTCT 
GAGGGGATAA CAACTTTGGA TAGGTTAAGT GTGGATGTCG TCAGTTGCGG ACATGACCAT 
TGTAACGATT ACTGTCTTCG AGATGACTCT ACGCCGAACA AAATCTGGCT ATGCTATGGC 
GGTGGAGGTG GAGAAGGTGG TTATGCTGGC TATGGAGGTA CAGAAAGACG TATCCGTATT 
TATGAAATTA ATGTAAATGA GAACAATATA CACACATGGA AAAGATTAAA TGGCAGTCCA 
AAAGAGATTT TTGATTTTCA GTCTATGTTG GACGGTAATT CTCCAGAATC TGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_013465.1
CDS	1..1737
Translation	MIRLPRLYQRYLLYLVVFVVIALFYFLQAPRVEEHIGFDLALPISHVDNLWFQNKGLEGFSNDDKLVVNIGYDECFHIGRFYEGCFNRHELKSTLTDGHQYLQRKRIHKDLRGSFGRRWFGKSEYLYYDVLYPALVDYFGSNLEKLNVEAVTGISKYPKDKSLPFMDVSITFEPISIELLQKRSYISDINILFGVDCIQPIANWTLQKEFPLVKYRYSEPAYLTYKFVGTRPVDTGAQRLQETDEGKFKIVQLADLHLGVGESECIDEYPKHEACKADPKTETFVQQVLDIEKPQLVVFTGDQIMGDRSIQDSETVLLKAVAPVIARKIPWAMVWGNHDDEGSLTRWQLSEIASVLPYSLFKFSPHDTHDNTFGVGNYIYQIFSNNDTEVPVGTLYFLDSHKYSTVGKIYPGYDWIKESQWKYIEDYHDVNLKFKTGLSMAFFHIPLPEYLNIESKTHPGEKNPLIGMYKEGVTAPKYNSEGITTLDRLSVDVVSCGHDHCNDYCLRDDSTPNKIWLCYGGGGGEGGYAGYGGTERRIRIYEINVNENNIHTWKRLNGSPKEIFDFQSMLDGNSPESV

Target ORF information:

RefSeq Version	NM_001182250.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C phosphoprotein phosphatase (DCR2), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001182250.1

ORF Insert Sequence:

ATGATCCGGT TACCGAGGCT GTATCAAAGG TACCTATTAT ACTTGGTAGT TTTTGTAGTG 
ATTGCCCTAT TTTACTTCCT ACAGGCGCCA AGAGTTGAAG AGCACATTGG TTTCGACCTT 
GCACTCCCGA TATCACACGT TGATAATCTA TGGTTCCAAA ATAAAGGTCT AGAGGGATTC 
TCAAATGACG ATAAGCTAGT TGTAAATATA GGCTATGACG AATGTTTTCA TATTGGTCGT 
TTTTATGAAG GATGTTTTAA TCGGCATGAA CTTAAATCAA CTTTAACTGA TGGGCACCAA 
TACTTACAAA GGAAAAGAAT TCATAAAGAC TTGAGAGGTT CCTTTGGACG CAGGTGGTTT 
GGAAAGTCAG AATACCTTTA CTATGATGTG TTATACCCTG CTTTGGTGGA CTACTTTGGA 
TCCAACCTAG AAAAGCTAAA TGTTGAGGCA GTAACTGGAA TTTCCAAATA TCCTAAAGAC 
AAATCGCTAC CCTTTATGGA TGTATCCATC ACATTCGAGC CTATTAGCAT AGAACTATTG 
CAGAAAAGGA GCTACATAAG CGATATTAAC ATTCTCTTTG GTGTTGATTG CATCCAGCCG 
ATAGCAAATT GGACTCTTCA AAAAGAATTT CCCTTAGTAA AATACCGCTA CAGCGAACCT 
GCTTATTTGA CTTACAAATT CGTTGGTACT CGTCCTGTAG ACACAGGAGC TCAACGGCTG 
CAAGAAACTG ATGAAGGAAA GTTCAAAATT GTTCAGTTGG CGGATCTCCA CTTGGGTGTG 
GGGGAGTCTG AATGTATCGA TGAGTATCCA AAGCATGAAG CTTGCAAGGC AGATCCAAAA 
ACGGAAACTT TCGTTCAACA AGTTCTTGAC ATTGAAAAAC CGCAGTTGGT TGTTTTTACT 
GGTGACCAAA TCATGGGTGA TAGATCTATT CAAGACTCTG AAACCGTATT ATTAAAGGCG 
GTAGCACCCG TCATTGCAAG AAAAATTCCG TGGGCAATGG TATGGGGAAA TCACGACGAC 
GAGGGAAGCT TAACGCGCTG GCAGTTATCC GAAATAGCTT CAGTACTACC ATATTCCCTT 
TTCAAGTTTA GTCCCCATGA TACACACGAT AATACGTTTG GCGTTGGTAA TTATATTTAT 
CAGATTTTTT CGAACAATGA TACAGAAGTC CCAGTAGGTA CACTTTATTT TCTGGACTCA 
CATAAATATT CTACGGTTGG TAAAATTTAT CCTGGATATG ATTGGATTAA GGAATCACAG 
TGGAAATACA TTGAAGATTA TCATGATGTG AACTTGAAGT TCAAAACAGG TCTTTCAATG 
GCATTTTTTC ACATCCCACT GCCAGAATAC TTGAATATTG AATCCAAGAC ACATCCCGGA 
GAGAAAAATC CCCTCATTGG AATGTACAAA GAAGGTGTTA CTGCACCTAA GTATAATTCT 
GAGGGGATAA CAACTTTGGA TAGGTTAAGT GTGGATGTCG TCAGTTGCGG ACATGACCAT 
TGTAACGATT ACTGTCTTCG AGATGACTCT ACGCCGAACA AAATCTGGCT ATGCTATGGC 
GGTGGAGGTG GAGAAGGTGG TTATGCTGGC TATGGAGGTA CAGAAAGACG TATCCGTATT 
TATGAAATTA ATGTAAATGA GAACAATATA CACACATGGA AAAGATTAAA TGGCAGTCCA 
AAAGAGATTT TTGATTTTCA GTCTATGTTG GACGGTAATT CTCCAGAATC TGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XII.
Nature387(6632 Suppl)87-90(1997 May)
Johnston M,Hillier L,Riles L,Albermann K,Andr? B,Ansorge W,Benes V,Br?ckner M,Delius H,Dubois E,D?sterh?ft A,Entian KD,Floeth M,Goffeau A,Hebling U,Heumann K,Heuss-Neitzel D,Hilbert H,Hilger F,Kleine K,K?tter P,Louis EJ,Messenguy F,Mewes HW,Hoheisel JD

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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DCR2 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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