FKS1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	FKS1
Entrez Gene ID	851055
Full Name	1,3-beta-D-glucan synthase
Synonyms	CND1,CWH53,ETG1,GSC1,PBR1
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001182231.1	NP_013446.1	1,3-beta-D-glucan synthase

Pathways from BioSystems

Homologies

Saccharomyces cerevisiae (baker's yeast)	GSC2	NP_011546.3
Saccharomyces cerevisiae (baker's yeast)	FKS1	NP_013446.1

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XII.
Johnston M, Hillier L, Riles L, Albermann K, Andr? B, Ansorge W, Benes V, Br?ckner M, Delius H, Dubois E, D?sterh?ft A, Entian KD, Floeth M, Goffeau A, Hebling U, Heumann K, Heuss-Neitzel D, Hilbert H, Hilger F, Kleine K, K?tter P, Louis EJ, Messenguy F, Mewes HW, Hoheisel JD
Nature387(6632 Suppl)87-90(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

the mitotic spindle breakage observed in endocytosis mutants can be rescued either by deleting or inhibiting the activities of, CHS2, CHS3 and FKS1, which are involved in septum formation. The findings from our study highlight the importance of timely endocytosis of cytokinetic enzymes at the division site in safeguarding mitotic spindle integrity during mitotic exit.
Title: Timely Endocytosis of Cytokinetic Enzymes Prevents Premature Spindle Breakage during Mitotic Exit.

Both the N terminus and the C terminus are essential for Fks1 function.
Title: Topological and mutational analysis of Saccharomyces cerevisiae Fks1.

Fks1p consists of multiple domains that are required for cell wall construction and cellular morphogenesis.
Title: Multiple functional domains of the yeast l,3-beta-glucan synthase subunit Fks1p revealed by quantitative phenotypic analysis of temperature-sensitive mutants.

The following FKS1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the FKS1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi00662	NM_001182231.1 Latest version!	Saccharomyces cerevisiae S288C 1,3-beta-D-glucan synthase (FKS1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

FKS1 ( NM_001182231.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_013446.1 Saccharomyces cerevisiae S288C 1,3-beta-D-glucan synthase (FKS1), partial mRNA.

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CloneID	OSi00662
Clone ID Related Accession (Same CDS sequence)	NM_001182231.1
Accession Version	NM_001182231.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5631bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	1,3-beta-D-glucan synthase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001144). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGAACACTG ATCAACAACC TTATCAGGGC CAAACGGACT ATACCCAGGG ACCAGGTAAC 
GGGCAAAGTC AGGAACAAGA CTATGACCAA TATGGCCAGC CTTTGTATCC TTCACAAGCT 
GATGGTTACT ACGATCCAAA TGTCGCTGCT GGTACTGAAG CTGATATGTA TGGTCAACAA 
CCACCAAACG AGTCTTACGA CCAAGACTAC ACAAACGGTG AATACTATGG TCAACCGCCA 
AATATGGCTG CTCAAGACGG TGAAAACTTC TCGGATTTTA GCAGTTACGG CCCTCCTGGA 
ACACCTGGAT ATGATAGCTA TGGTGGTCAG TATACCGCTT CTCAAATGAG TTATGGAGAA 
CCAAATTCGT CGGGTACCTC GACTCCAATT TACGGTAATT ATGACCCAAA TGCTATCGCT 
ATGGCTTTGC CAAATGAACC TTATCCCGCT TGGACTGCTG ACTCTCAATC TCCCGTTTCG 
ATCGAGCAAA TCGAAGATAT CTTTATTGAT TTGACCAACA GACTCGGGTT CCAAAGAGAC 
TCCATGAGAA ATATGTTTGA TCATTTTATG GTTCTCTTGG ACTCTAGGTC CTCGAGAATG 
TCTCCTGATC AAGCTTTACT ATCTTTACAT GCCGACTACA TTGGTGGCGA TACTGCTAAC 
TATAAAAAAT GGTATTTTGC TGCTCAGTTA GATATGGATG ATGAAATTGG TTTTAGAAAT 
ATGAGTCTTG GAAAACTCTC AAGGAAGGCA AGAAAAGCTA AGAAGAAAAA CAAGAAAGCA 
ATGGAAGAGG CCAATCCCGA AGACACTGAA GAAACTTTAA ACAAAATTGA AGGCGACAAC 
TCCCTAGAGG CTGCTGATTT TAGATGGAAG GCCAAGATGA ACCAGTTGTC TCCCCTGGAA 
AGAGTTCGTC ATATCGCCTT ATATCTGTTA TGTTGGGGTG AAGCTAATCA AGTCAGATTC 
ACTGCTGAAT GTTTATGTTT TATCTACAAG TGTGCTCTTG ACTACTTGGA TTCCCCTCTT 
TGCCAACAAC GCCAAGAACC TATGCCAGAA GGTGATTTCT TGAATAGAGT CATTACGCCA 
ATTTATCATT TCATCAGAAA TCAAGTTTAT GAAATTGTTG ATGGTCGTTT TGTCAAGCGT 
GAAAGAGATC ATAACAAAAT TGTCGGTTAT GATGATTTAA ACCAATTGTT CTGGTATCCA 
GAAGGTATTG CAAAGATTGT TCTTGAAGAT GGAACAAAAT TGATAGAACT CCCATTGGAA 
GAACGTTATT TAAGATTAGG CGATGTCGTC TGGGATGATG TATTCTTCAA AACATATAAA 
GAGACCCGTA CTTGGTTACA TTTGGTCACC AACTTCAACC GTATTTGGGT TATGCATATC 
TCCATTTTTT GGATGTACTT TGCATATAAT TCACCAACAT TTTACACTCA TAACTATCAA 
CAATTGGTCG ACAACCAACC TTTGGCTGCT TACAAGTGGG CATCTTGCGC ATTAGGTGGT 
ACTGTCGCAA GTTTGATTCA AATTGTCGCT ACTTTGTGTG AATGGTCATT CGTTCCAAGA 
AAATGGGCTG GTGCTCAACA TCTATCTCGT AGATTCTGGT TTTTATGCAT CATCTTTGGT 
ATTAATTTGG GTCCTATTAT TTTTGTTTTT GCTTACGACA AAGATACAGT CTACTCCACT 
GCTGCACACG TTGTTGCTGC TGTTATGTTC TTTGTTGCGG TTGCTACCAT CATATTCTTC 
TCCATTATGC CATTGGGGGG GTTGTTTACG TCATATATGA AAAAATCTAC AAGGCGTTAT 
GTTGCATCTC AAACATTCAC TGCTGCATTT GCCCCTCTAC ATGGGTTAGA TAGATGGATG 
TCCTATTTAG TTTGGGTTAC TGTTTTTGCT GCCAAATATT CAGAATCGTA CTACTTTTTA 
GTTTTATCTT TGAGAGATCC AATTAGAATT TTGTCCACCA CTGCAATGAG GTGTACAGGT 
GAATACTGGT GGGGTGCGGT ACTTTGTAAA GTGCAACCCA AGATTGTCTT AGGTTTGGTT 
ATCGCTACCG ACTTCATTCT TTTCTTCTTG GATACCTACT TATGGTACAT TATTGTGAAT 
ACCATTTTCT CTGTTGGGAA ATCTTTCTAT TTAGGTATTT CTATCTTAAC ACCATGGAGA 
AATATCTTCA CAAGATTGCC AAAAAGAATA TACTCCAAGA TTTTGGCTAC TACTGATATG 
GAAATTAAAT ACAAACCAAA GGTTTTGATT TCTCAAGTAT GGAATGCCAT CATTATTTCA 
ATGTACAGAG AACATCTCTT AGCCATCGAC CATGTACAAA AATTACTATA TCATCAAGTT 
CCATCTGAAA TCGAAGGTAA AAGAACTTTG AGAGCTCCTA CCTTCTTTGT TTCTCAAGAT 
GACAATAATT TTGAGACTGA ATTTTTCCCT AGGGATTCAG AGGCTGAGCG TCGTATTTCT 
TTCTTTGCTC AATCTTTGTC TACTCCAATT CCCGAACCAC TTCCAGTTGA TAACATGCCA 
ACGTTCACAG TATTGACTCC TCACTACGCG GAAAGAATTC TGCTGTCATT AAGAGAAATT 
ATTCGTGAAG ATGACCAATT TTCTAGAGTT ACTCTTTTAG AATATCTAAA ACAATTACAT 
CCCGTTGAAT GGGAATGTTT TGTTAAGGAT ACTAAGATTT TGGCTGAAGA AACCGCTGCC 
TATGAAGGAA ATGAAAATGA AGCTGAAAAG GAAGATGCTT TGAAATCTCA AATCGATGAT 
TTGCCATTTT ATTGTATTGG TTTTAAATCT GCTGCTCCAG AATATACACT TCGTACGAGA 
ATTTGGGCTT CTTTGAGGTC GCAGACTCTA TATCGTACCA TTTCAGGGTT CATGAATTAT 
TCAAGAGCTA TCAAATTACT GTATCGTGTG GAAAATCCTG AAATTGTTCA AATGTTTGGT 
GGTAATGCTG AAGGCTTAGA AAGAGAGCTA GAAAAGATGG CAAGAAGAAA GTTTAAATTT 
TTGGTCTCTA TGCAGAGATT GGCTAAATTC AAACCACATG AACTGGAAAA TGCTGAGTTT 
TTGTTGAGAG CTTACCCAGA CTTACAAATT GCCTACTTGG ATGAAGAGCC ACCTTTGACT 
GAAGGTGAGG AGCCAAGAAT CTATTCCGCT TTGATTGATG GACATTGTGA AATTCTAGAT 
AATGGTCGTA GACGTCCCAA GTTTAGAGTT CAATTATCTG GTAACCCAAT TCTTGGTGAC 
GGTAAATCTG ATAACCAAAA CCATGCTTTG ATTTTTTACA GAGGTGAATA CATTCAATTA 
ATTGATGCCA ACCAAGATAA CTACTTGGAA GAATGTCTGA AGATTAGATC TGTATTGGCT 
GAATTTGAGG AATTGAACGT TGAACAAGTT AATCCATATG CTCCCGGTTT AAGGTATGAG 
GAGCAAACAA CTAATCATCC TGTTGCTATT GTTGGTGCCA GAGAATACAT TTTCTCTGAA 
AACTCTGGTG TGCTGGGTGA TGTGGCCGCT GGTAAAGAAC AAACTTTTGG TACATTATTT 
GCGCGTACTT TATCTCAAAT TGGTGGTAAA TTGCATTATG GTCATCCGGA TTTCATTAAT 
GCTACGTTTA TGACCACTAG AGGTGGTGTT TCCAAAGCAC AAAAGGGTTT GCATTTAAAC 
GAAGATATTT ATGCTGGTAT GAATGCTATG CTTCGTGGTG GTCGTATCAA GCATTGTGAG 
TATTATCAAT GTGGTAAAGG TAGAGATTTG GGTTTCGGTA CAATTCTAAA TTTCACTACT 
AAGATTGGTG CTGGTATGGG TGAACAAATG TTATCTCGTG AATATTATTA TCTGGGTACC 
CAATTACCAG TGGACCGTTT CCTAACATTC TATTATGCCC ATCCTGGTTT CCATTTGAAC 
AACTTGTTCA TTCAATTATC TTTGCAAATG TTTATGTTGA CTTTGGTGAA TTTATCTTCC 
TTGGCCCATG AATCTATTAT GTGTATTTAC GATAGGAACA AACCAAAAAC AGATGTTTTG 
GTTCCAATTG GGTGTTACAA CTTCCAACCT GCGGTTGATT GGGTGAGACG TTATACATTG 
TCTATTTTCA TTGTTTTCTG GATTGCCTTC GTTCCTATTG TTGTTCAAGA ACTAATTGAA 
CGTGGTCTAT GGAAAGCCAC CCAAAGATTT TTCTGCCACC TATTATCATT ATCCCCTATG 
TTCGAAGTGT TTGCGGGCCA AATCTACTCT TCTGCGTTAT TAAGTGATTT AGCAATTGGT 
GGTGCTCGTT ATATATCCAC CGGTCGTGGT TTTGCAACTT CTCGTATACC ATTTTCAATT 
TTGTATTCAA GATTTGCAGG ATCTGCTATC TACATGGGTG CAAGATCAAT GTTAATGTTG 
CTGTTCGGTA CTGTCGCACA TTGGCAAGCT CCACTACTGT GGTTTTGGGC CTCTCTATCT 
TCATTAATTT TTGCGCCTTT CGTTTTCAAT CCACATCAGT TTGCTTGGGA AGATTTCTTT 
TTGGATTACA GGGATTATAT CAGATGGTTA TCAAGAGGTA ATAATCAATA TCATAGAAAC 
TCGTGGATTG GTTACGTGAG GATGTCTAGG GCACGTATTA CTGGGTTTAA ACGTAAACTG 
GTTGGCGATG AATCTGAGAA AGCTGCTGGT GACGCAAGCA GGGCTCATAG AACCAATTTG 
ATCATGGCTG AAATCATACC CTGTGCAATT TATGCAGCTG GTTGTTTTAT TGCCTTCACG 
TTTATTAATG CTCAAACCGG TGTCAAGACT ACTGATGATG ATAGGGTGAA TTCTGTTTTA 
CGTATCATCA TTTGTACCTT GGCGCCAATC GCCGTTAACC TCGGTGTTCT ATTCTTCTGT 
ATGGGTATGT CATGCTGCTC TGGTCCCTTA TTTGGTATGT GTTGTAAGAA GACAGGTTCT 
GTAATGGCTG GAATTGCCCA CGGTGTTGCT GTTATTGTCC ACATTGCCTT TTTCATTGTC 
ATGTGGGTTT TGGAGAGCTT CAACTTTGTT AGAATGTTAA TCGGAGTCGT TACTTGTATC 
CAATGTCAAA GACTCATTTT TCATTGCATG ACAGCGTTAA TGTTGACTCG TGAATTTAAA 
AACGATCATG CCAATACAGC CTTCTGGACT GGTAAGTGGT ATGGTAAAGG TATGGGTTAC 
ATGGCTTGGA CCCAGCCAAG TAGAGAATTA ACCGCCAAGG TAATTGAGCT TTCAGAATTT 
GCAGCTGATT TTGTTCTAGG TCATGTGATT TTAATCTGTC AACTGCCACT CATTATAATC 
CCAAAAATAG ATAAATTCCA CTCGATTATG CTATTCTGGC TAAAGCCCTC TCGTCAAATT 
CGTCCCCCAA TTTACTCTCT GAAGCAAACT CGTTTGCGTA AGCGTATGGT CAAGAAGTAC 
TGCTCTTTGT ACTTTTTAGT ATTGGCTATT TTTGCAGGAT GCATTATTGG TCCTGCTGTA 
GCCTCTGCTA AGATCCACAA ACACATTGGA GATTCATTGG ATGGCGTTGT TCACAATCTA 
TTCCAACCAA TAAATACAAC CAATAATGAC ACTGGTTCCC AAATGTCAAC TTATCAAAGT 
CACTACTATA CTCATACGCC ATCATTAAAG ACCTGGTCAA CTATAAAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_013446.1
CDS	1..5631
Translation	MNTDQQPYQGQTDYTQGPGNGQSQEQDYDQYGQPLYPSQADGYYDPNVAAGTEADMYGQQPPNESYDQDYTNGEYYGQPPNMAAQDGENFSDFSSYGPPGTPGYDSYGGQYTASQMSYGEPNSSGTSTPIYGNYDPNAIAMALPNEPYPAWTADSQSPVSIEQIEDIFIDLTNRLGFQRDSMRNMFDHFMVLLDSRSSRMSPDQALLSLHADYIGGDTANYKKWYFAAQLDMDDEIGFRNMSLGKLSRKARKAKKKNKKAMEEANPEDTEETLNKIEGDNSLEAADFRWKAKMNQLSPLERVRHIALYLLCWGEANQVRFTAECLCFIYKCALDYLDSPLCQQRQEPMPEGDFLNRVITPIYHFIRNQVYEIVDGRFVKRERDHNKIVGYDDLNQLFWYPEGIAKIVLEDGTKLIELPLEERYLRLGDVVWDDVFFKTYKETRTWLHLVTNFNRIWVMHISIFWMYFAYNSPTFYTHNYQQLVDNQPLAAYKWASCALGGTVASLIQIVATLCEWSFVPRKWAGAQHLSRRFWFLCIIFGINLGPIIFVFAYDKDTVYSTAAHVVAAVMFFVAVATIIFFSIMPLGGLFTSYMKKSTRRYVASQTFTAAFAPLHGLDRWMSYLVWVTVFAAKYSESYYFLVLSLRDPIRILSTTAMRCTGEYWWGAVLCKVQPKIVLGLVIATDFILFFLDTYLWYIIVNTIFSVGKSFYLGISILTPWRNIFTRLPKRIYSKILATTDMEIKYKPKVLISQVWNAIIISMYREHLLAIDHVQKLLYHQVPSEIEGKRTLRAPTFFVSQDDNNFETEFFPRDSEAERRISFFAQSLSTPIPEPLPVDNMPTFTVLTPHYAERILLSLREIIREDDQFSRVTLLEYLKQLHPVEWECFVKDTKILAEETAAYEGNENEAEKEDALKSQIDDLPFYCIGFKSAAPEYTLRTRIWASLRSQTLYRTISGFMNYSRAIKLLYRVENPEIVQMFGGNAEGLERELEKMARRKFKFLVSMQRLAKFKPHELENAEFLLRAYPDLQIAYLDEEPPLTEGEEPRIYSALIDGHCEILDNGRRRPKFRVQLSGNPILGDGKSDNQNHALIFYRGEYIQLIDANQDNYLEECLKIRSVLAEFEELNVEQVNPYAPGLRYEEQTTNHPVAIVGAREYIFSENSGVLGDVAAGKEQTFGTLFARTLSQIGGKLHYGHPDFINATFMTTRGGVSKAQKGLHLNEDIYAGMNAMLRGGRIKHCEYYQCGKGRDLGFGTILNFTTKIGAGMGEQMLSREYYYLGTQLPVDRFLTFYYAHPGFHLNNLFIQLSLQMFMLTLVNLSSLAHESIMCIYDRNKPKTDVLVPIGCYNFQPAVDWVRRYTLSIFIVFWIAFVPIVVQELIERGLWKATQRFFCHLLSLSPMFEVFAGQIYSSALLSDLAIGGARYISTGRGFATSRIPFSILYSRFAGSAIYMGARSMLMLLFGTVAHWQAPLLWFWASLSSLIFAPFVFNPHQFAWEDFFLDYRDYIRWLSRGNNQYHRNSWIGYVRMSRARITGFKRKLVGDESEKAAGDASRAHRTNLIMAEIIPCAIYAAGCFIAFTFINAQTGVKTTDDDRVNSVLRIIICTLAPIAVNLGVLFFCMGMSCCSGPLFGMCCKKTGSVMAGIAHGVAVIVHIAFFIVMWVLESFNFVRMLIGVVTCIQCQRLIFHCMTALMLTREFKNDHANTAFWTGKWYGKGMGYMAWTQPSRELTAKVIELSEFAADFVLGHVILICQLPLIIIPKIDKFHSIMLFWLKPSRQIRPPIYSLKQTRLRKRMVKKYCSLYFLVLAIFAGCIIGPAVASAKIHKHIGDSLDGVVHNLFQPINTTNNDTGSQMSTYQSHYYTHTPSLKTWSTIK

Target ORF information:

RefSeq Version	NM_001182231.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C 1,3-beta-D-glucan synthase (FKS1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001182231.1

ORF Insert Sequence:

ATGAACACTG ATCAACAACC TTATCAGGGC CAAACGGACT ATACCCAGGG ACCAGGTAAC 
GGGCAAAGTC AGGAACAAGA CTATGACCAA TATGGCCAGC CTTTGTATCC TTCACAAGCT 
GATGGTTACT ACGATCCAAA TGTCGCTGCT GGTACTGAAG CTGATATGTA TGGTCAACAA 
CCACCAAACG AGTCTTACGA CCAAGACTAC ACAAACGGTG AATACTATGG TCAACCGCCA 
AATATGGCTG CTCAAGACGG TGAAAACTTC TCGGATTTTA GCAGTTACGG CCCTCCTGGA 
ACACCTGGAT ATGATAGCTA TGGTGGTCAG TATACCGCTT CTCAAATGAG TTATGGAGAA 
CCAAATTCGT CGGGTACCTC GACTCCAATT TACGGTAATT ATGACCCAAA TGCTATCGCT 
ATGGCTTTGC CAAATGAACC TTATCCCGCT TGGACTGCTG ACTCTCAATC TCCCGTTTCG 
ATCGAGCAAA TCGAAGATAT CTTTATTGAT TTGACCAACA GACTCGGGTT CCAAAGAGAC 
TCCATGAGAA ATATGTTTGA TCATTTTATG GTTCTCTTGG ACTCTAGGTC CTCGAGAATG 
TCTCCTGATC AAGCTTTACT ATCTTTACAT GCCGACTACA TTGGTGGCGA TACTGCTAAC 
TATAAAAAAT GGTATTTTGC TGCTCAGTTA GATATGGATG ATGAAATTGG TTTTAGAAAT 
ATGAGTCTTG GAAAACTCTC AAGGAAGGCA AGAAAAGCTA AGAAGAAAAA CAAGAAAGCA 
ATGGAAGAGG CCAATCCCGA AGACACTGAA GAAACTTTAA ACAAAATTGA AGGCGACAAC 
TCCCTAGAGG CTGCTGATTT TAGATGGAAG GCCAAGATGA ACCAGTTGTC TCCCCTGGAA 
AGAGTTCGTC ATATCGCCTT ATATCTGTTA TGTTGGGGTG AAGCTAATCA AGTCAGATTC 
ACTGCTGAAT GTTTATGTTT TATCTACAAG TGTGCTCTTG ACTACTTGGA TTCCCCTCTT 
TGCCAACAAC GCCAAGAACC TATGCCAGAA GGTGATTTCT TGAATAGAGT CATTACGCCA 
ATTTATCATT TCATCAGAAA TCAAGTTTAT GAAATTGTTG ATGGTCGTTT TGTCAAGCGT 
GAAAGAGATC ATAACAAAAT TGTCGGTTAT GATGATTTAA ACCAATTGTT CTGGTATCCA 
GAAGGTATTG CAAAGATTGT TCTTGAAGAT GGAACAAAAT TGATAGAACT CCCATTGGAA 
GAACGTTATT TAAGATTAGG CGATGTCGTC TGGGATGATG TATTCTTCAA AACATATAAA 
GAGACCCGTA CTTGGTTACA TTTGGTCACC AACTTCAACC GTATTTGGGT TATGCATATC 
TCCATTTTTT GGATGTACTT TGCATATAAT TCACCAACAT TTTACACTCA TAACTATCAA 
CAATTGGTCG ACAACCAACC TTTGGCTGCT TACAAGTGGG CATCTTGCGC ATTAGGTGGT 
ACTGTCGCAA GTTTGATTCA AATTGTCGCT ACTTTGTGTG AATGGTCATT CGTTCCAAGA 
AAATGGGCTG GTGCTCAACA TCTATCTCGT AGATTCTGGT TTTTATGCAT CATCTTTGGT 
ATTAATTTGG GTCCTATTAT TTTTGTTTTT GCTTACGACA AAGATACAGT CTACTCCACT 
GCTGCACACG TTGTTGCTGC TGTTATGTTC TTTGTTGCGG TTGCTACCAT CATATTCTTC 
TCCATTATGC CATTGGGGGG GTTGTTTACG TCATATATGA AAAAATCTAC AAGGCGTTAT 
GTTGCATCTC AAACATTCAC TGCTGCATTT GCCCCTCTAC ATGGGTTAGA TAGATGGATG 
TCCTATTTAG TTTGGGTTAC TGTTTTTGCT GCCAAATATT CAGAATCGTA CTACTTTTTA 
GTTTTATCTT TGAGAGATCC AATTAGAATT TTGTCCACCA CTGCAATGAG GTGTACAGGT 
GAATACTGGT GGGGTGCGGT ACTTTGTAAA GTGCAACCCA AGATTGTCTT AGGTTTGGTT 
ATCGCTACCG ACTTCATTCT TTTCTTCTTG GATACCTACT TATGGTACAT TATTGTGAAT 
ACCATTTTCT CTGTTGGGAA ATCTTTCTAT TTAGGTATTT CTATCTTAAC ACCATGGAGA 
AATATCTTCA CAAGATTGCC AAAAAGAATA TACTCCAAGA TTTTGGCTAC TACTGATATG 
GAAATTAAAT ACAAACCAAA GGTTTTGATT TCTCAAGTAT GGAATGCCAT CATTATTTCA 
ATGTACAGAG AACATCTCTT AGCCATCGAC CATGTACAAA AATTACTATA TCATCAAGTT 
CCATCTGAAA TCGAAGGTAA AAGAACTTTG AGAGCTCCTA CCTTCTTTGT TTCTCAAGAT 
GACAATAATT TTGAGACTGA ATTTTTCCCT AGGGATTCAG AGGCTGAGCG TCGTATTTCT 
TTCTTTGCTC AATCTTTGTC TACTCCAATT CCCGAACCAC TTCCAGTTGA TAACATGCCA 
ACGTTCACAG TATTGACTCC TCACTACGCG GAAAGAATTC TGCTGTCATT AAGAGAAATT 
ATTCGTGAAG ATGACCAATT TTCTAGAGTT ACTCTTTTAG AATATCTAAA ACAATTACAT 
CCCGTTGAAT GGGAATGTTT TGTTAAGGAT ACTAAGATTT TGGCTGAAGA AACCGCTGCC 
TATGAAGGAA ATGAAAATGA AGCTGAAAAG GAAGATGCTT TGAAATCTCA AATCGATGAT 
TTGCCATTTT ATTGTATTGG TTTTAAATCT GCTGCTCCAG AATATACACT TCGTACGAGA 
ATTTGGGCTT CTTTGAGGTC GCAGACTCTA TATCGTACCA TTTCAGGGTT CATGAATTAT 
TCAAGAGCTA TCAAATTACT GTATCGTGTG GAAAATCCTG AAATTGTTCA AATGTTTGGT 
GGTAATGCTG AAGGCTTAGA AAGAGAGCTA GAAAAGATGG CAAGAAGAAA GTTTAAATTT 
TTGGTCTCTA TGCAGAGATT GGCTAAATTC AAACCACATG AACTGGAAAA TGCTGAGTTT 
TTGTTGAGAG CTTACCCAGA CTTACAAATT GCCTACTTGG ATGAAGAGCC ACCTTTGACT 
GAAGGTGAGG AGCCAAGAAT CTATTCCGCT TTGATTGATG GACATTGTGA AATTCTAGAT 
AATGGTCGTA GACGTCCCAA GTTTAGAGTT CAATTATCTG GTAACCCAAT TCTTGGTGAC 
GGTAAATCTG ATAACCAAAA CCATGCTTTG ATTTTTTACA GAGGTGAATA CATTCAATTA 
ATTGATGCCA ACCAAGATAA CTACTTGGAA GAATGTCTGA AGATTAGATC TGTATTGGCT 
GAATTTGAGG AATTGAACGT TGAACAAGTT AATCCATATG CTCCCGGTTT AAGGTATGAG 
GAGCAAACAA CTAATCATCC TGTTGCTATT GTTGGTGCCA GAGAATACAT TTTCTCTGAA 
AACTCTGGTG TGCTGGGTGA TGTGGCCGCT GGTAAAGAAC AAACTTTTGG TACATTATTT 
GCGCGTACTT TATCTCAAAT TGGTGGTAAA TTGCATTATG GTCATCCGGA TTTCATTAAT 
GCTACGTTTA TGACCACTAG AGGTGGTGTT TCCAAAGCAC AAAAGGGTTT GCATTTAAAC 
GAAGATATTT ATGCTGGTAT GAATGCTATG CTTCGTGGTG GTCGTATCAA GCATTGTGAG 
TATTATCAAT GTGGTAAAGG TAGAGATTTG GGTTTCGGTA CAATTCTAAA TTTCACTACT 
AAGATTGGTG CTGGTATGGG TGAACAAATG TTATCTCGTG AATATTATTA TCTGGGTACC 
CAATTACCAG TGGACCGTTT CCTAACATTC TATTATGCCC ATCCTGGTTT CCATTTGAAC 
AACTTGTTCA TTCAATTATC TTTGCAAATG TTTATGTTGA CTTTGGTGAA TTTATCTTCC 
TTGGCCCATG AATCTATTAT GTGTATTTAC GATAGGAACA AACCAAAAAC AGATGTTTTG 
GTTCCAATTG GGTGTTACAA CTTCCAACCT GCGGTTGATT GGGTGAGACG TTATACATTG 
TCTATTTTCA TTGTTTTCTG GATTGCCTTC GTTCCTATTG TTGTTCAAGA ACTAATTGAA 
CGTGGTCTAT GGAAAGCCAC CCAAAGATTT TTCTGCCACC TATTATCATT ATCCCCTATG 
TTCGAAGTGT TTGCGGGCCA AATCTACTCT TCTGCGTTAT TAAGTGATTT AGCAATTGGT 
GGTGCTCGTT ATATATCCAC CGGTCGTGGT TTTGCAACTT CTCGTATACC ATTTTCAATT 
TTGTATTCAA GATTTGCAGG ATCTGCTATC TACATGGGTG CAAGATCAAT GTTAATGTTG 
CTGTTCGGTA CTGTCGCACA TTGGCAAGCT CCACTACTGT GGTTTTGGGC CTCTCTATCT 
TCATTAATTT TTGCGCCTTT CGTTTTCAAT CCACATCAGT TTGCTTGGGA AGATTTCTTT 
TTGGATTACA GGGATTATAT CAGATGGTTA TCAAGAGGTA ATAATCAATA TCATAGAAAC 
TCGTGGATTG GTTACGTGAG GATGTCTAGG GCACGTATTA CTGGGTTTAA ACGTAAACTG 
GTTGGCGATG AATCTGAGAA AGCTGCTGGT GACGCAAGCA GGGCTCATAG AACCAATTTG 
ATCATGGCTG AAATCATACC CTGTGCAATT TATGCAGCTG GTTGTTTTAT TGCCTTCACG 
TTTATTAATG CTCAAACCGG TGTCAAGACT ACTGATGATG ATAGGGTGAA TTCTGTTTTA 
CGTATCATCA TTTGTACCTT GGCGCCAATC GCCGTTAACC TCGGTGTTCT ATTCTTCTGT 
ATGGGTATGT CATGCTGCTC TGGTCCCTTA TTTGGTATGT GTTGTAAGAA GACAGGTTCT 
GTAATGGCTG GAATTGCCCA CGGTGTTGCT GTTATTGTCC ACATTGCCTT TTTCATTGTC 
ATGTGGGTTT TGGAGAGCTT CAACTTTGTT AGAATGTTAA TCGGAGTCGT TACTTGTATC 
CAATGTCAAA GACTCATTTT TCATTGCATG ACAGCGTTAA TGTTGACTCG TGAATTTAAA 
AACGATCATG CCAATACAGC CTTCTGGACT GGTAAGTGGT ATGGTAAAGG TATGGGTTAC 
ATGGCTTGGA CCCAGCCAAG TAGAGAATTA ACCGCCAAGG TAATTGAGCT TTCAGAATTT 
GCAGCTGATT TTGTTCTAGG TCATGTGATT TTAATCTGTC AACTGCCACT CATTATAATC 
CCAAAAATAG ATAAATTCCA CTCGATTATG CTATTCTGGC TAAAGCCCTC TCGTCAAATT 
CGTCCCCCAA TTTACTCTCT GAAGCAAACT CGTTTGCGTA AGCGTATGGT CAAGAAGTAC 
TGCTCTTTGT ACTTTTTAGT ATTGGCTATT TTTGCAGGAT GCATTATTGG TCCTGCTGTA 
GCCTCTGCTA AGATCCACAA ACACATTGGA GATTCATTGG ATGGCGTTGT TCACAATCTA 
TTCCAACCAA TAAATACAAC CAATAATGAC ACTGGTTCCC AAATGTCAAC TTATCAAAGT 
CACTACTATA CTCATACGCC ATCATTAAAG ACCTGGTCAA CTATAAAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XII.
Nature387(6632 Suppl)87-90(1997 May)
Johnston M,Hillier L,Riles L,Albermann K,Andr? B,Ansorge W,Benes V,Br?ckner M,Delius H,Dubois E,D?sterh?ft A,Entian KD,Floeth M,Goffeau A,Hebling U,Heumann K,Heuss-Neitzel D,Hilbert H,Hilger F,Kleine K,K?tter P,Louis EJ,Messenguy F,Mewes HW,Hoheisel JD

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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FKS1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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