ORF » Species Summary » Saccharomyces cerevisiae S288C » SPH1 cDNA ORF clone

SPH1 cDNA ORF clone, Saccharomyces cerevisiae S288C

  • Gene

  • Clones

  • gRNAs

The following SPH1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SPH1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OSi00639 NM_001182202.2
Latest version!		 Saccharomyces cerevisiae S288C Sph1p (SPH1), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    SPH1 ( NM_001182202.2 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_013417.3 Saccharomyces cerevisiae S288C Sph1p (SPH1), partial mRNA.
    Price & Availability↑
    CloneID OSi00639
    Clone ID Related Accession (Same CDS sequence) NM_001182202.2
    Accession Version NM_001182202.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1593bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-31
    Organism Saccharomyces cerevisiae S288C
    Product Sph1p
    Comment Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001144). On Apr 26, 2011 this sequence version replaced NM_001182202.1. ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGATTAGCT CAGAATTAAC GAATGATCAA ATCATAGACC TTATATCGGA TTATAAGAAT 
    TTCAAAAGAA TTATAGAGGC CTCTGTACCA GAAGATGATA GAAGAAGGAA CCAAAACCGC 
    CAAAATAGAA ATAAAGACCT GACAAAATTG TCGAATGTAC AATTTTGGCA ACTGACGACT 
    GATGTCAACG ATGAATTGAT GAAAAGATTG ACAGATTCGG GTGCGGATGC ATCACTAAAT 
    GATCTTGATC TGAAAAGAGG TAAAGCACAA TCTAAACTGT CTCGATTGAA GGACGCGAAG 
    TTCCATAAAT TGATTCTTGA TATTTTCACG GAAATTGAAA GGAGAAACTT ACATCATTTG 
    GATATGGGGA CGCATAACAA TGGGTTAGAC GAGGGTGATC TAAATTTCTA TTTAAATGAC 
    ACATTATTTG AATCCTTCAA AATAAACGAT GATTTTATGA GCGTGAATGG TATTATATCC 
    ATTGAAGTCT TCCGAGAATT AAAAACTCAA TTTACACTTT ATTTTCAAAA CACGCTCCAT 
    CGAATTGATC CTGTGGATAC CACCACCACA CGCCTGCCGA TTCTGCTTGA GACTATAATT 
    AAGATAGCAA AGTTGATAGG CGATCTCTTA CCTGTCCTAT CATCAGTTTC ACTGCAAAGT 
    TCTTTAGAAA ACGAAATAGT ATACTTAAAG AGTGCTTTAT CTCATGCTAT CACTTCAACA 
    AGATATTTTC TCACATATGG GGACCTAATA CCACGAATTG TAGCTCAATC ATCTATTTCG 
    GAAGTGATTT TTGCGTTCTG CAATATTGCT CAGATTGTGA AGATCAAATC CACCTCACGT 
    GATGATATAT CACGCAATGA AGGAGAACTC AGTGATATCG AGGCAGGTAT GAAACCGTTG 
    AAGATTATTG AAAAAGTGAA AAATGAGAAA AACGGTAAGG ATATATCATC TCTGGGTGAT 
    AGCGGTAGTA CTGCTGTTAG TTTTCCATCC TCTGGTAAGC CCATTACAAA AAAATCGGAC 
    ATGCCAGTGG TTGTAGCATC GCCATCAATT TCCATCATAG AAAAATCTGA ATCTTCTATT 
    CGAGAGAGTG GGAAGGTACG AAATAATACA TCTGGAGAAA CAAACTTAGC GTCTGTGTCG 
    CCGTTAAAAA ACACTAAGAA TTCTTCAAGA ATTACGAGTG AGCCATCTCC GCGAGAAGGT 
    TTGCCTCTGA AAGTTGTCTC GAACTCGAGA AGTCCAAGTC CTCAAGGTAA CACATTGCCG 
    CTAATTGGTA AATTTCGGCA GGATTATCAG GCATCACCTC CGAAGAAAGT TATCACTAAA 
    CCAGTGGCAG AAACGGCGAA GCCGTATGCA AATATTCCGC CAGCTGCAGA CGTACTTTAC 
    TCGCCAACCG TGACCAAAAT GAGGAAATTC AGAGAGAAAG TGCAGAAATT TGCTCCCAAC 
    TCTGGTCTTG GATTAAGAAT ATCTACTTCA GAAGAAAACT TGAATAATTC TGATGTCAAC 
    TCAACCACGC ACAACGCTAA CATAAATAAC CTCGTCGAAT TTGTTGAGAG TAAATCAATG 
    GTTGTTCTTC CTATGGCAGG CAGGGCATTT TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_013417.3
    CDS1..1593
    Translation

    Target ORF information:

    RefSeq Version NM_001182202.2
    Organism Saccharomyces cerevisiae S288C
    Definition Saccharomyces cerevisiae S288C Sph1p (SPH1), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001182202.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGATTAGCT CAGAATTAAC GAATGATCAA ATCATAGACC TTATATCGGA TTATAAGAAT 
    TTCAAAAGAA TTATAGAGGC CTCTGTACCA GAAGATGATA GAAGAAGGAA CCAAAACCGC 
    CAAAATAGAA ATAAAGACCT GACAAAATTG TCGAATGTAC AATTTTGGCA ACTGACGACT 
    GATGTCAACG ATGAATTGAT GAAAAGATTG ACAGATTCGG GTGCGGATGC ATCACTAAAT 
    GATCTTGATC TGAAAAGAGG TAAAGCACAA TCTAAACTGT CTCGATTGAA GGACGCGAAG 
    TTCCATAAAT TGATTCTTGA TATTTTCACG GAAATTGAAA GGAGAAACTT ACATCATTTG 
    GATATGGGGA CGCATAACAA TGGGTTAGAC GAGGGTGATC TAAATTTCTA TTTAAATGAC 
    ACATTATTTG AATCCTTCAA AATAAACGAT GATTTTATGA GCGTGAATGG TATTATATCC 
    ATTGAAGTCT TCCGAGAATT AAAAACTCAA TTTACACTTT ATTTTCAAAA CACGCTCCAT 
    CGAATTGATC CTGTGGATAC CACCACCACA CGCCTGCCGA TTCTGCTTGA GACTATAATT 
    AAGATAGCAA AGTTGATAGG CGATCTCTTA CCTGTCCTAT CATCAGTTTC ACTGCAAAGT 
    TCTTTAGAAA ACGAAATAGT ATACTTAAAG AGTGCTTTAT CTCATGCTAT CACTTCAACA 
    AGATATTTTC TCACATATGG GGACCTAATA CCACGAATTG TAGCTCAATC ATCTATTTCG 
    GAAGTGATTT TTGCGTTCTG CAATATTGCT CAGATTGTGA AGATCAAATC CACCTCACGT 
    GATGATATAT CACGCAATGA AGGAGAACTC AGTGATATCG AGGCAGGTAT GAAACCGTTG 
    AAGATTATTG AAAAAGTGAA AAATGAGAAA AACGGTAAGG ATATATCATC TCTGGGTGAT 
    AGCGGTAGTA CTGCTGTTAG TTTTCCATCC TCTGGTAAGC CCATTACAAA AAAATCGGAC 
    ATGCCAGTGG TTGTAGCATC GCCATCAATT TCCATCATAG AAAAATCTGA ATCTTCTATT 
    CGAGAGAGTG GGAAGGTACG AAATAATACA TCTGGAGAAA CAAACTTAGC GTCTGTGTCG 
    CCGTTAAAAA ACACTAAGAA TTCTTCAAGA ATTACGAGTG AGCCATCTCC GCGAGAAGGT 
    TTGCCTCTGA AAGTTGTCTC GAACTCGAGA AGTCCAAGTC CTCAAGGTAA CACATTGCCG 
    CTAATTGGTA AATTTCGGCA GGATTATCAG GCATCACCTC CGAAGAAAGT TATCACTAAA 
    CCAGTGGCAG AAACGGCGAA GCCGTATGCA AATATTCCGC CAGCTGCAGA CGTACTTTAC 
    TCGCCAACCG TGACCAAAAT GAGGAAATTC AGAGAGAAAG TGCAGAAATT TGCTCCCAAC 
    TCTGGTCTTG GATTAAGAAT ATCTACTTCA GAAGAAAACT TGAATAATTC TGATGTCAAC 
    TCAACCACGC ACAACGCTAA CATAAATAAC CTCGTCGAAT TTGTTGAGAG TAAATCAATG 
    GTTGTTCTTC CTATGGCAGG CAGGGCATTT TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The nucleotide sequence of Saccharomyces cerevisiae chromosome XII.
    Nature387(6632 Suppl)87-90(1997 May)
    Johnston M,Hillier L,Riles L,Albermann K,Andr? B,Ansorge W,Benes V,Br?ckner M,Delius H,Dubois E,D?sterh?ft A,Entian KD,Floeth M,Goffeau A,Hebling U,Heumann K,Heuss-Neitzel D,Hilbert H,Hilger F,Kleine K,K?tter P,Louis EJ,Messenguy F,Mewes HW,Hoheisel JD


    Life with 6000 genes.
    Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
    Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG


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