CDC25 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	CDC25
Entrez Gene ID	851019
Full Name	Ras family guanine nucleotide exchange factor CDC25
Synonyms	CTN1
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001182198.1	NP_013413.1	Ras family guanine nucleotide exchange factor CDC25

Pathways from BioSystems

Homologies

Saccharomyces cerevisiae (baker's yeast)	CDC25	NP_013413.1
Kluyveromyces lactis	KLLA0D09306g	XP_453477.1
Eremothecium gossypii	AGOS_ADL038W	NP_984058.2

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XII.
Johnston M, Hillier L, Riles L, Albermann K, Andr? B, Ansorge W, Benes V, Br?ckner M, Delius H, Dubois E, D?sterh?ft A, Entian KD, Floeth M, Goffeau A, Hebling U, Heumann K, Heuss-Neitzel D, Hilbert H, Hilger F, Kleine K, K?tter P, Louis EJ, Messenguy F, Mewes HW, Hoheisel JD
Nature387(6632 Suppl)87-90(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Carried out stochastic simulations, in particular, the effect of the negative feedback loops on the activity of both Ira2 (a Ras-GAP) and Cdc25 (a Ras-GEF) proteins.
Title: Simulation of the Ras/cAMP/PKA pathway in budding yeast highlights the establishment of stable oscillatory states.

Data show that the Ras2p localization is regulated by PKA, and that PKA down-regulates Ras2p activity and the protein association between Cdc25p and Ras2-GTP, which is due to a reduced Ras2-GEF Cdc25p activity.
Title: The membrane localization of Ras2p and the association between Cdc25p and Ras2-GTP are regulated by protein kinase A (PKA) in the yeast Saccharomyces cerevisiae.

Glucose-induced Ras2 activation was investigated in mutants lacking the N-terminal domain of Cdc25 or the entire CDC25; results suggest that the signal did not sustain the glucose-induced increase of Ras2-GTP normally observed in a wild-type strain.
Title: The large N-terminal domain of Cdc25 protein of the yeast Saccharomyces cerevisiae is required for glucose-induced Ras2 activation.

Taken together, these results suggest that Cdc25 is involved in the regulation of cell size in the presence of different carbon sources.
Title: The N-terminal region of the Saccharomyces cerevisiae RasGEF Cdc25 is required for nutrient-dependent cell-size regulation.

The following CDC25 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CDC25 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi00636	NM_001182198.1 Latest version!	Saccharomyces cerevisiae S288C Ras family guanine nucleotide exchange factor CDC25 (CDC25), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CDC25 ( NM_001182198.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_013413.1 Saccharomyces cerevisiae S288C Ras family guanine nucleotide exchange factor CDC25 (CDC25), partial mRNA.

Price & Availability↑

CloneID	OSi00636
Clone ID Related Accession (Same CDS sequence)	NM_001182198.1
Accession Version	NM_001182198.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4770bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	Ras family guanine nucleotide exchange factor CDC25
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001144). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGTCCGATA CTAACACGTC TATTCCCAAT ACAAGTTCTG CAAGGGAGGC AGGCAATGCT 
TCACAAACTC CATCGATCAG CTCTTCATCT AACACTTCCA CTACCACTAA CACAGAATCA 
TCCTCAGCTT CTCTTTCTTC TTCCCCCTCG ACAAGTGAGT TGACCAGCAT TCGTCCAATT 
GGAATAGTAG TCGCTGCTTA TGACTTTAAT TATCCCATTA AAAAAGACAG TTCTTCGCAA 
CTTTTGTCTG TACAACAAGG GGAAACCATT TATATACTTA ACAAAAACTC ATCTGGGTGG 
TGGGATGGAT TAGTTATTGA CGACAGTAAT GGGAAAGTTA ACAGAGGCTG GTTTCCTCAA 
AACTTCGGTA GACCTTTAAG AGACAGTCAT CTCAGAAAGC ACAGTCATCC GATGAAAAAA 
TATAGTTCCA GTAAGAGCTC AAGGCGCAGC AGCTTAAATA GCTTGGGCAA TAGTGCATAT 
TTACATGTGC CTAGAAATCC GAGCAAGAGC AGGAGGGGGA GTTCTACTTT ATCAGCGTCT 
TTATCAAATG CCCACAATGC AGAAACAAGT TCCGGCCACA ATAACACCGT ATCGATGAAT 
AATTCTCCCT TTTCAGCGCC AAACGATGCT TCCCACATAA CCCCTCAATC TTCGAACTTT 
AATTCCAATG CTAGTCTATC CCAGGATATG ACAAAGAGTG CAGATGGCTC ATCTGAGATG 
AATACAAACG CAATTATGAA TAACAATGAA ACAAATTTAC AAACTTCTGG TGAGAAAGCA 
GGTCCCCCAC TAGTAGCAGA AGAAACAATT AAGATATTAC CGTTGGAAGA GATAGAAATG 
ATTATTAATG GTATACGTTC GAACATTGCT TCGACTTGGT CCCCCATACC ACTGATAACG 
AAAACATCCG ATTACAAGTT GGTATACTAT AACAAAGACC TTGATATATA CTGTTCAGAA 
TTACCCTTGA TTTCTAACTC AATTATGGAA TCCGATGACA TTTGTGACAG CGAACCAAAA 
TTCCCGCCCA ATGATCATCT TGTTAACCTA TATACTAGAG ATCTGAGGAA AAATGCGAAT 
ATTGAGGACA GTTCTACGAG ATCGAAGCAA TCGGAAAGTG AACAAAATAG ATCAAGCCTT 
CTAATGGAAA AACAGGATTC AAAAGAAACT GATGGAAATA ATAACAGTAT TAATGATGAT 
GATAATAATA ACGAAAATAA CAAAAACGAA TTCAATGAGG CTGGTCCTTC ATCATTAAAT 
TCTTTATCTG CTCCAGATTT AACGCAGAAT ATTCAATCAA GGGTAGTTGC CCCAAGTCGC 
TCTTCTATAC TGGCCAAGAG TGACATCTTT TATCACTATT CAAGAGATAT AAAATTGTGG 
ACAGAATTAC AAGACCTAAC AGTTTATTAT ACTAAAACGG CTCACAAGAT GTTCCTTAAA 
GAGAATCGTC TCAATTTCAC GAAATACTTT GATTTGATAT CAGATTCAAT AGTCTTCACA 
CAGTTAGGCT GCAGGCTAAT GCAACATGAA ATTAAAGCCA AAAGTTGCAG CAAGGAGATT 
AAGAAGATTT TCAAAGGTCT AATCTCTTCA TTGTCAAGGA TAAGTATCAA TTCTCATTTA 
TATTTCGATT CAGCTTTTCA CAGAAAAAAA ATGGATACTA TGAATGACAA GGATAACGAT 
AATCAGGAAA ATAATTGTTC TAGGACGGAA GGGGATGATG GTAAAATTGA AGTAGATAGT 
GTACATGATC TAGTTTCAGT TCCATTGTCC GGTAAACGTA ATGTAAGTAC CAGTACAACG 
GATACATTGA CTCCAATGAG ATCATCATTC AGTACAGTCA ATGAGAACGA TATGGAAAAT 
TTCTCAGTCT TAGGTCCAAG AAATAGTGTT AATTCTGTCG TAACACCAAG GACTTCAATA 
CAAAATTCTA CTTTGGAAGA TTTTTCACCG TCCAACAAAA ATTTTAAGTC AGCTAAATCG 
ATTTACGAAA TGGTTGATGT GGAATTCTCG AAATTTTTAA GGCATGTTCA GTTACTTTAT 
TTTGTGTTAC AGAGCTCAGT CTTCTCAGAT GATAATACTT TACCACAGTT GCTCCCAAGA 
TTTTTTAAAG GTTCATTTAG CGGTGGTTCT TGGACAAATC CATTTTCGAC TTTTATTACG 
GATGAATTTG GTAATGCGAC AAAGAACAAA GCTGTCACAT CTAATGAAGT GACCGCTTCG 
TCGTCCAAAA ATTCCTCAAT ATCAAGGATT CCACCAAAGA TGGCAGATGC TATTGCCTCT 
GCGTCAGGAT ATAGCGCTAA TTCAGAAACA AATTCCCAAA TTGATTTAAA AGCAAGCAGT 
GCCGCGTCTG GTTCAGTTTT TACACCTTTC AACCGTCCTT CTCATAACAG AACCTTTTCA 
AGAGCAAGAG TTTCAAAAAG GAAGAAAAAA TATCCATTAA CTGTAGACAC TTTGAATACA 
ATGAAGAAGA AATCCTCGCA AATTTTTGAA AAATTAAATA ATGCTACAGG TGAACACTTA 
AAAATTATAA GTAAACCCAA AAGCAGAATT AGGAATTTGG AAATAAATTC AAGCACATAC 
GAACAAATAA ATCAGAATGT TTTACTATTG GAGATACTGG AGAATTTAGA TCTGTCAATT 
TTCATCAATT TGAAAAACCT GATTAAGACA CCCAGTATTT TGTTGGATTT GGAAAGCGAG 
GAATTTTTAG TTCACGCCAT GTCTTCGGTC TCCTCAGTAC TAACAGAGTT TTTTGATATA 
AAGCAGGCTT TTCATGACAT CGTCATCAGA TTAATAATGA CAACGCAACA AACGACCTTA 
GACGACCCGT ATTTGTTTTC CTCAATGAGG TCCAATTTCC CTGTCGGACA TCATGAACCT 
TTCAAGAATA TCTCTAATAC ACCTTTGGTC AAGGGCCCCT TCCATAAAAA AAATGAACAA 
TTGGCACTCT CCTTATTTCA CGTATTGGTG AGTCAAGATG TGGAGTTCAA TAACCTTGAA 
TTTTTAAACA ACTCCGACGA TTTTAAAGAT GCTTGTGAAA AGTATGTCGA GATTTCTAAT 
CTTGCGTGTA TTATTGTTGA TCAATTGATT GAAGAAAGAG AAAATTTGCT GAACTACGCA 
GCAAGAATGA TGAAGAATAA TTTGACTGCA GAACTATTGA AAGGTGAGCA AGAAAAATGG 
TTTGATATTT ATTCCGAGGA CTATAGTGAT GACGATTCAG AAAATGATGA AGCTATCATC 
GATGACGAAT TAGGATCTGA GGACTATATT GAACGCAAAG CTGCGAACAT AGAGAAAAAC 
CTTCCATGGT TTTTAACTTC AGATTATGAA ACTAGTCTTG TCTATGACTC AAGAGGAAAA 
ATTCGTGGCG GGACAAAAGA GGCACTGATT GAACATTTAA CCAGTCATGA ACTTGTTGAT 
GCGGCTTTCA ATGTTACAAT GTTAATAACT TTCAGAAGTA TATTAACCAC AAGAGAGTTT 
TTTTATGCCC TGATTTACAG GTACAACTTG TATCCTCCTG AAGGGCTGAG TTACGATGAT 
TACAATATTT GGATAGAAAA AAAGTCAAAC CCGATTAAAT GCCGTGTGGT CAACATTATG 
AGAACATTTT TGACGCAGTA TTGGACAAGA AATTATTATG AACCTGGCAT ACCACTGATT 
CTAAATTTTG CCAAGATGGT TGTATCGGAG AAAATACCGG GGGCAGAGGA TCTTTTGCAA 
AAGATAAATG AAAAACTGAT AAATGAGAAT GAGAAAGAAC CAGTGGATCC TAAGCAACAA 
GATTCGGTAT CGGCAGTCGT ACAGACAACT AAACGTGACA ATAAATCACC GATACACATG 
TCTTCGTCTT CTTTACCATC TTCTGCTTCT TCAGCGTTTT TTAGATTGAA GAAATTGAAG 
CTCTTGGATA TTGACCCATA CACATATGCC ACACAATTGA CTGTACTTGA ACATGACTTA 
TACCTCAGGA TCACTATGTT TGAATGCTTG GATAGGGCAT GGGGTACCAA GTATTGTAAT 
ATGGGTGGTT CTCCGAACAT TACGAAATTT ATAGCTAATG CTAATACGCT AACTAATTTT 
GTTTCTCATA CCATTGTAAA ACAGGCAGAT GTCAAGACAC GTTCAAAATT AACGCAATAT 
TTTGTTACCG TTGCCCAGCA TTGTAAAGAG TTGAATAATT TTTCTTCAAT GACTGCCATA 
GTGTCCGCTT TGTATTCCTC CCCAATCTAC CGACTGAAAA AGACATGGGA TTTAGTTTCC 
ACTGAGTCGA AGGACCTTCT GAAGAACCTA AACAACCTTA TGGATTCCAA GAGAAATTTT 
GTGAAGTATA GAGAGCTGTT GCGATCCGTG ACGGACGTTG CATGTGTTCC CTTTTTTGGT 
GTATACCTAT CTGATTTAAC ATTTACGTTT GTCGGAAACC CAGATTTTCT TCACAATTCA 
ACCAACATAA TAAACTTCAG CAAGAGGACT AAAATCGCAA ATATAGTGGA GGAAATTATA 
AGCTTTAAAA GATTCCATTA CAAGCTGAAA CGATTGGATG ATATTCAGAC CGTTATAGAA 
GCGTCTTTGG AAAATGTCCC CCACATTGAA AAGCAGTATC AATTATCACT GCAAGTGGAA 
CCGAGATCAG GAAACACCAA AGGCAGTACG CATGCTTCTT CTGCTAGCGG TACAAAAACT 
GCAAAATTCC TAAGTGAATT TACAGATGAT AAAAATGGCA ATTTTTTGAA GCTAGGTAAG 
AAAAAACCTC CTTCTAGGTT ATTTCGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_013413.1
CDS	1..4770
Translation	MSDTNTSIPNTSSAREAGNASQTPSISSSSNTSTTTNTESSSASLSSSPSTSELTSIRPIGIVVAAYDFNYPIKKDSSSQLLSVQQGETIYILNKNSSGWWDGLVIDDSNGKVNRGWFPQNFGRPLRDSHLRKHSHPMKKYSSSKSSRRSSLNSLGNSAYLHVPRNPSKSRRGSSTLSASLSNAHNAETSSGHNNTVSMNNSPFSAPNDASHITPQSSNFNSNASLSQDMTKSADGSSEMNTNAIMNNNETNLQTSGEKAGPPLVAEETIKILPLEEIEMIINGIRSNIASTWSPIPLITKTSDYKLVYYNKDLDIYCSELPLISNSIMESDDICDSEPKFPPNDHLVNLYTRDLRKNANIEDSSTRSKQSESEQNRSSLLMEKQDSKETDGNNNSINDDDNNNENNKNEFNEAGPSSLNSLSAPDLTQNIQSRVVAPSRSSILAKSDIFYHYSRDIKLWTELQDLTVYYTKTAHKMFLKENRLNFTKYFDLISDSIVFTQLGCRLMQHEIKAKSCSKEIKKIFKGLISSLSRISINSHLYFDSAFHRKKMDTMNDKDNDNQENNCSRTEGDDGKIEVDSVHDLVSVPLSGKRNVSTSTTDTLTPMRSSFSTVNENDMENFSVLGPRNSVNSVVTPRTSIQNSTLEDFSPSNKNFKSAKSIYEMVDVEFSKFLRHVQLLYFVLQSSVFSDDNTLPQLLPRFFKGSFSGGSWTNPFSTFITDEFGNATKNKAVTSNEVTASSSKNSSISRIPPKMADAIASASGYSANSETNSQIDLKASSAASGSVFTPFNRPSHNRTFSRARVSKRKKKYPLTVDTLNTMKKKSSQIFEKLNNATGEHLKIISKPKSRIRNLEINSSTYEQINQNVLLLEILENLDLSIFINLKNLIKTPSILLDLESEEFLVHAMSSVSSVLTEFFDIKQAFHDIVIRLIMTTQQTTLDDPYLFSSMRSNFPVGHHEPFKNISNTPLVKGPFHKKNEQLALSLFHVLVSQDVEFNNLEFLNNSDDFKDACEKYVEISNLACIIVDQLIEERENLLNYAARMMKNNLTAELLKGEQEKWFDIYSEDYSDDDSENDEAIIDDELGSEDYIERKAANIEKNLPWFLTSDYETSLVYDSRGKIRGGTKEALIEHLTSHELVDAAFNVTMLITFRSILTTREFFYALIYRYNLYPPEGLSYDDYNIWIEKKSNPIKCRVVNIMRTFLTQYWTRNYYEPGIPLILNFAKMVVSEKIPGAEDLLQKINEKLINENEKEPVDPKQQDSVSAVVQTTKRDNKSPIHMSSSSLPSSASSAFFRLKKLKLLDIDPYTYATQLTVLEHDLYLRITMFECLDRAWGTKYCNMGGSPNITKFIANANTLTNFVSHTIVKQADVKTRSKLTQYFVTVAQHCKELNNFSSMTAIVSALYSSPIYRLKKTWDLVSTESKDLLKNLNNLMDSKRNFVKYRELLRSVTDVACVPFFGVYLSDLTFTFVGNPDFLHNSTNIINFSKRTKIANIVEEIISFKRFHYKLKRLDDIQTVIEASLENVPHIEKQYQLSLQVEPRSGNTKGSTHASSASGTKTAKFLSEFTDDKNGNFLKLGKKKPPSRLFR

Target ORF information:

RefSeq Version	NM_001182198.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C Ras family guanine nucleotide exchange factor CDC25 (CDC25), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001182198.1

ORF Insert Sequence:

ATGTCCGATA CTAACACGTC TATTCCCAAT ACAAGTTCTG CAAGGGAGGC AGGCAATGCT 
TCACAAACTC CATCGATCAG CTCTTCATCT AACACTTCCA CTACCACTAA CACAGAATCA 
TCCTCAGCTT CTCTTTCTTC TTCCCCCTCG ACAAGTGAGT TGACCAGCAT TCGTCCAATT 
GGAATAGTAG TCGCTGCTTA TGACTTTAAT TATCCCATTA AAAAAGACAG TTCTTCGCAA 
CTTTTGTCTG TACAACAAGG GGAAACCATT TATATACTTA ACAAAAACTC ATCTGGGTGG 
TGGGATGGAT TAGTTATTGA CGACAGTAAT GGGAAAGTTA ACAGAGGCTG GTTTCCTCAA 
AACTTCGGTA GACCTTTAAG AGACAGTCAT CTCAGAAAGC ACAGTCATCC GATGAAAAAA 
TATAGTTCCA GTAAGAGCTC AAGGCGCAGC AGCTTAAATA GCTTGGGCAA TAGTGCATAT 
TTACATGTGC CTAGAAATCC GAGCAAGAGC AGGAGGGGGA GTTCTACTTT ATCAGCGTCT 
TTATCAAATG CCCACAATGC AGAAACAAGT TCCGGCCACA ATAACACCGT ATCGATGAAT 
AATTCTCCCT TTTCAGCGCC AAACGATGCT TCCCACATAA CCCCTCAATC TTCGAACTTT 
AATTCCAATG CTAGTCTATC CCAGGATATG ACAAAGAGTG CAGATGGCTC ATCTGAGATG 
AATACAAACG CAATTATGAA TAACAATGAA ACAAATTTAC AAACTTCTGG TGAGAAAGCA 
GGTCCCCCAC TAGTAGCAGA AGAAACAATT AAGATATTAC CGTTGGAAGA GATAGAAATG 
ATTATTAATG GTATACGTTC GAACATTGCT TCGACTTGGT CCCCCATACC ACTGATAACG 
AAAACATCCG ATTACAAGTT GGTATACTAT AACAAAGACC TTGATATATA CTGTTCAGAA 
TTACCCTTGA TTTCTAACTC AATTATGGAA TCCGATGACA TTTGTGACAG CGAACCAAAA 
TTCCCGCCCA ATGATCATCT TGTTAACCTA TATACTAGAG ATCTGAGGAA AAATGCGAAT 
ATTGAGGACA GTTCTACGAG ATCGAAGCAA TCGGAAAGTG AACAAAATAG ATCAAGCCTT 
CTAATGGAAA AACAGGATTC AAAAGAAACT GATGGAAATA ATAACAGTAT TAATGATGAT 
GATAATAATA ACGAAAATAA CAAAAACGAA TTCAATGAGG CTGGTCCTTC ATCATTAAAT 
TCTTTATCTG CTCCAGATTT AACGCAGAAT ATTCAATCAA GGGTAGTTGC CCCAAGTCGC 
TCTTCTATAC TGGCCAAGAG TGACATCTTT TATCACTATT CAAGAGATAT AAAATTGTGG 
ACAGAATTAC AAGACCTAAC AGTTTATTAT ACTAAAACGG CTCACAAGAT GTTCCTTAAA 
GAGAATCGTC TCAATTTCAC GAAATACTTT GATTTGATAT CAGATTCAAT AGTCTTCACA 
CAGTTAGGCT GCAGGCTAAT GCAACATGAA ATTAAAGCCA AAAGTTGCAG CAAGGAGATT 
AAGAAGATTT TCAAAGGTCT AATCTCTTCA TTGTCAAGGA TAAGTATCAA TTCTCATTTA 
TATTTCGATT CAGCTTTTCA CAGAAAAAAA ATGGATACTA TGAATGACAA GGATAACGAT 
AATCAGGAAA ATAATTGTTC TAGGACGGAA GGGGATGATG GTAAAATTGA AGTAGATAGT 
GTACATGATC TAGTTTCAGT TCCATTGTCC GGTAAACGTA ATGTAAGTAC CAGTACAACG 
GATACATTGA CTCCAATGAG ATCATCATTC AGTACAGTCA ATGAGAACGA TATGGAAAAT 
TTCTCAGTCT TAGGTCCAAG AAATAGTGTT AATTCTGTCG TAACACCAAG GACTTCAATA 
CAAAATTCTA CTTTGGAAGA TTTTTCACCG TCCAACAAAA ATTTTAAGTC AGCTAAATCG 
ATTTACGAAA TGGTTGATGT GGAATTCTCG AAATTTTTAA GGCATGTTCA GTTACTTTAT 
TTTGTGTTAC AGAGCTCAGT CTTCTCAGAT GATAATACTT TACCACAGTT GCTCCCAAGA 
TTTTTTAAAG GTTCATTTAG CGGTGGTTCT TGGACAAATC CATTTTCGAC TTTTATTACG 
GATGAATTTG GTAATGCGAC AAAGAACAAA GCTGTCACAT CTAATGAAGT GACCGCTTCG 
TCGTCCAAAA ATTCCTCAAT ATCAAGGATT CCACCAAAGA TGGCAGATGC TATTGCCTCT 
GCGTCAGGAT ATAGCGCTAA TTCAGAAACA AATTCCCAAA TTGATTTAAA AGCAAGCAGT 
GCCGCGTCTG GTTCAGTTTT TACACCTTTC AACCGTCCTT CTCATAACAG AACCTTTTCA 
AGAGCAAGAG TTTCAAAAAG GAAGAAAAAA TATCCATTAA CTGTAGACAC TTTGAATACA 
ATGAAGAAGA AATCCTCGCA AATTTTTGAA AAATTAAATA ATGCTACAGG TGAACACTTA 
AAAATTATAA GTAAACCCAA AAGCAGAATT AGGAATTTGG AAATAAATTC AAGCACATAC 
GAACAAATAA ATCAGAATGT TTTACTATTG GAGATACTGG AGAATTTAGA TCTGTCAATT 
TTCATCAATT TGAAAAACCT GATTAAGACA CCCAGTATTT TGTTGGATTT GGAAAGCGAG 
GAATTTTTAG TTCACGCCAT GTCTTCGGTC TCCTCAGTAC TAACAGAGTT TTTTGATATA 
AAGCAGGCTT TTCATGACAT CGTCATCAGA TTAATAATGA CAACGCAACA AACGACCTTA 
GACGACCCGT ATTTGTTTTC CTCAATGAGG TCCAATTTCC CTGTCGGACA TCATGAACCT 
TTCAAGAATA TCTCTAATAC ACCTTTGGTC AAGGGCCCCT TCCATAAAAA AAATGAACAA 
TTGGCACTCT CCTTATTTCA CGTATTGGTG AGTCAAGATG TGGAGTTCAA TAACCTTGAA 
TTTTTAAACA ACTCCGACGA TTTTAAAGAT GCTTGTGAAA AGTATGTCGA GATTTCTAAT 
CTTGCGTGTA TTATTGTTGA TCAATTGATT GAAGAAAGAG AAAATTTGCT GAACTACGCA 
GCAAGAATGA TGAAGAATAA TTTGACTGCA GAACTATTGA AAGGTGAGCA AGAAAAATGG 
TTTGATATTT ATTCCGAGGA CTATAGTGAT GACGATTCAG AAAATGATGA AGCTATCATC 
GATGACGAAT TAGGATCTGA GGACTATATT GAACGCAAAG CTGCGAACAT AGAGAAAAAC 
CTTCCATGGT TTTTAACTTC AGATTATGAA ACTAGTCTTG TCTATGACTC AAGAGGAAAA 
ATTCGTGGCG GGACAAAAGA GGCACTGATT GAACATTTAA CCAGTCATGA ACTTGTTGAT 
GCGGCTTTCA ATGTTACAAT GTTAATAACT TTCAGAAGTA TATTAACCAC AAGAGAGTTT 
TTTTATGCCC TGATTTACAG GTACAACTTG TATCCTCCTG AAGGGCTGAG TTACGATGAT 
TACAATATTT GGATAGAAAA AAAGTCAAAC CCGATTAAAT GCCGTGTGGT CAACATTATG 
AGAACATTTT TGACGCAGTA TTGGACAAGA AATTATTATG AACCTGGCAT ACCACTGATT 
CTAAATTTTG CCAAGATGGT TGTATCGGAG AAAATACCGG GGGCAGAGGA TCTTTTGCAA 
AAGATAAATG AAAAACTGAT AAATGAGAAT GAGAAAGAAC CAGTGGATCC TAAGCAACAA 
GATTCGGTAT CGGCAGTCGT ACAGACAACT AAACGTGACA ATAAATCACC GATACACATG 
TCTTCGTCTT CTTTACCATC TTCTGCTTCT TCAGCGTTTT TTAGATTGAA GAAATTGAAG 
CTCTTGGATA TTGACCCATA CACATATGCC ACACAATTGA CTGTACTTGA ACATGACTTA 
TACCTCAGGA TCACTATGTT TGAATGCTTG GATAGGGCAT GGGGTACCAA GTATTGTAAT 
ATGGGTGGTT CTCCGAACAT TACGAAATTT ATAGCTAATG CTAATACGCT AACTAATTTT 
GTTTCTCATA CCATTGTAAA ACAGGCAGAT GTCAAGACAC GTTCAAAATT AACGCAATAT 
TTTGTTACCG TTGCCCAGCA TTGTAAAGAG TTGAATAATT TTTCTTCAAT GACTGCCATA 
GTGTCCGCTT TGTATTCCTC CCCAATCTAC CGACTGAAAA AGACATGGGA TTTAGTTTCC 
ACTGAGTCGA AGGACCTTCT GAAGAACCTA AACAACCTTA TGGATTCCAA GAGAAATTTT 
GTGAAGTATA GAGAGCTGTT GCGATCCGTG ACGGACGTTG CATGTGTTCC CTTTTTTGGT 
GTATACCTAT CTGATTTAAC ATTTACGTTT GTCGGAAACC CAGATTTTCT TCACAATTCA 
ACCAACATAA TAAACTTCAG CAAGAGGACT AAAATCGCAA ATATAGTGGA GGAAATTATA 
AGCTTTAAAA GATTCCATTA CAAGCTGAAA CGATTGGATG ATATTCAGAC CGTTATAGAA 
GCGTCTTTGG AAAATGTCCC CCACATTGAA AAGCAGTATC AATTATCACT GCAAGTGGAA 
CCGAGATCAG GAAACACCAA AGGCAGTACG CATGCTTCTT CTGCTAGCGG TACAAAAACT 
GCAAAATTCC TAAGTGAATT TACAGATGAT AAAAATGGCA ATTTTTTGAA GCTAGGTAAG 
AAAAAACCTC CTTCTAGGTT ATTTCGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XII.
Nature387(6632 Suppl)87-90(1997 May)
Johnston M,Hillier L,Riles L,Albermann K,Andr? B,Ansorge W,Benes V,Br?ckner M,Delius H,Dubois E,D?sterh?ft A,Entian KD,Floeth M,Goffeau A,Hebling U,Heumann K,Heuss-Neitzel D,Hilbert H,Hilger F,Kleine K,K?tter P,Louis EJ,Messenguy F,Mewes HW,Hoheisel JD

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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CDC25 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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