STT4 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	STT4
Entrez Gene ID	851014
Full Name	1-phosphatidylinositol 4-kinase STT4
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001182193.1	NP_013408.1	1-phosphatidylinositol 4-kinase STT4

Pathways from BioSystems

Homologies

Schizosaccharomyces pombe (fission yeast)	stt4	NP_595304.1
Saccharomyces cerevisiae (baker's yeast)	STT4	NP_013408.1
Magnaporthe oryzae (rice blast fungus)	MGG_13790	XP_003710881.1
Kluyveromyces lactis	KLLA0C17292g	XP_452973.1
Eremothecium gossypii	AGOS_ADL033W	NP_984064.1
Neurospora crassa	NCU09367	XP_963777.1

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XII.
Johnston M, Hillier L, Riles L, Albermann K, Andr? B, Ansorge W, Benes V, Br?ckner M, Delius H, Dubois E, D?sterh?ft A, Entian KD, Floeth M, Goffeau A, Hebling U, Heumann K, Heuss-Neitzel D, Hilbert H, Hilger F, Kleine K, K?tter P, Louis EJ, Messenguy F, Mewes HW, Hoheisel JD
Nature387(6632 Suppl)87-90(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

inositol-containing sphingolipid metabolism controls PKC signaling by regulating access of the signaling lipids PI4P and phosphatidylinositol 4,5-bisphosphate to effector proteins on the plasma membrane
Title: Interruption of inositol sphingolipid synthesis triggers Stt4p-dependent protein kinase C signaling.

Ypp1 and Efr3 are required for the formation and architecture of Stt4 PIK patches and ultimately plasma membrane-based PtdIns4P signaling.
Title: Assembly of the PtdIns 4-kinase Stt4 complex at the plasma membrane requires Ypp1 and Efr3.

overexpression of STT4 suppresses the temperature-sensitive growth defect of ypp1 mutants.
Title: Ypp1/YGR198w plays an essential role in phosphoinositide signalling at the plasma membrane.

The following STT4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the STT4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi00631	NM_001182193.1 Latest version!	Saccharomyces cerevisiae S288C 1-phosphatidylinositol 4-kinase STT4 (STT4), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

STT4 ( NM_001182193.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_013408.1 Saccharomyces cerevisiae S288C 1-phosphatidylinositol 4-kinase STT4 (STT4), partial mRNA.

Price & Availability↑

CloneID	OSi00631
Clone ID Related Accession (Same CDS sequence)	NM_001182193.1
Accession Version	NM_001182193.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5703bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	1-phosphatidylinositol 4-kinase STT4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001144). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGAGATTTA CCAGAGGATT GAAAGCCTCT TCATCTTTAA GAGCGAAAGC TATTGGAAGA 
CTAACTAAAC TTTCGACGGG TGCACCAAAT GATCAAAACA GTAATGGTAC AACATTAGAT 
TTGATAACTC ATACATTGCC TATTTTTTAT TCCACAAATA CGTCAAAGAT ATATACTATA 
CCACTTACAT TGAGTGAATG GGAAGTACTA ACTTCGCTTT GTGTTGCAAT CCCTACCACG 
CTCGATCTGG TAGAGACAAT GCTTAAAGAA ATAATTGCGC CTTATTTTCT GGAAACTCCA 
AGACAAAGAA TTTCAGATGT TCTGTCCTCG AAATTCAAGT TGGAACAGAT GAGAAATCCG 
ATTGAATTGC TAACTTTCCA GCTGACTAAG TTTATGATTC AAGCTTGTGA ACAGTATCCC 
GTTCTTTATG AAAATATAGG CGGCATCATT TCAACTTATT TTGAGCGTGT ATTAAAGATT 
TTTACTATTA AACAATCTGG TCTACTATCA TTAGTGGGCT TTATTAATGC ATTTATCCAG 
TTTCCCAACT CTACAGAGCT TACTAAATTC ACCTGGAAAA AGTTAGCGAA ACTTGTGCTT 
CGTGGATCAT TTCTAAACGA AGTTGATAAG ATTTTGAATT CCTCAGCAAC GTTTACCAAT 
GATTCAATCG TCCAGTATTA CGATGCTGGA AATGAGCTAT CCAGTGCCTA CTTATTGGAA 
TTAATATCTC GCTTGCAAGT TTCGCTAATA TCTCATCTGT TAAACACTTC ACATGTCGGC 
GCCAACTTGA GTGAGTTCTT ACTAAATCAA CAATACCAAT TTTACAAATT TGATCAAGAA 
GTAGCCGATG AAAACGATGA TACGAAGTGT ATTGATGACT TTTTTTTCAA CGTAAGAAGC 
AATAAACAGT TTTTTACAGA CATGTGTAAA ATTTCCCTGC AATTTTGTTC TGAGTCACAT 
ATTCTCGACC TATCTACAGA CAACCGTGCA AGATTTTCTT TTGATACTCG AGCCCACTAC 
CTACAAACGC TATGTTTAAT TCCGTTTATA GAAGATACAG AAAGCGAGCT TTTTGAAAGT 
TTTACAAACG TTGTTTCTGA ATCTATTGAT AAATTTTTCT TATCTGATGT CGTTACACCA 
TCTTTGATAA AAGCAATTGT AGCTTCTGCC TCATTACTGA ATTTTTTCAC GGAAAAATTG 
TCATTAACTC TGATTCGTAT GTTTCCTTTA TTGGTAGCGT CTCCACATAT CACGACTGAA 
ACAGTTAATG ATGTTGCAAA AATATTCACC ACAGGGTTAT ACCCTCTAAA TGAAGATGCA 
ATTGTGAGTA CAATTTATTC CATGAACAAC TTATTAGCTG TTTCCGAGGA CGGATCGCCC 
GTCCCGGTGC TCCGTGAGCG CCAATTAACA ATAACATCTG GAAAAAATAT TGAGAAAGAC 
TACTTTCCCT TGCGAAATTC ATCTGCCAGT TTGGATGGCA CTGGTGCTCT GCTCGGAAAT 
ACAACTGTGG GCCAACTTTC CTCCCATGAT GTCAATAGTG GAGCTACTAT GACATACCAT 
GCCTCTTTGA TATCTAATTG TGTGGCTGCA ACGACTACAA TCGCCTCATA CTATAACACC 
CAAAGCATAA CAGCTTTGAC TATCTCTATT CTTACGCAAA AAGTTAATTC TATGTCAAAG 
GAATTAGACG GTGTTATTTT GAATTCTTTG GCAAGACTGG CTCCGAATAC TTCACTGACA 
GAATTTTCGC TTTTACTAAA GTTCTTCAAA TCGAGAACTG TTATTGCAAC AAAAATTGAT 
GATAGCGCAC TATTGAAAAA TATTATTAAA GCGAAATGTG TGATATCAAA AGAATTGTTG 
GCCAGGCATT TTTCAAGTGA CTTATATTTC ATGTATCTTC ATGATCTATT GGACTCAATC 
ATTGCCAGTG GGGAAGTAGA ACGATTGGAA CATCACAGAC CTCAAACGGA GATTTCCCGT 
GTTGCTGATC AAATCGCTAC CTACTTAGAG CCCCTCGCCG CTCTACTACC TGTTCCAGGC 
GATACGCCAT TGGACATCAA TAAGGATGAA GTCACTACAA ACAAGTTTAG GAACGCCTGG 
TTTAATTTTG TCATCCATGG GTACCACTTG GGCGGGCCTA TTGTCAAACG AAATTTTTCT 
TTCTTATTAA CTATTGCCTA TAATTCACCA CCGCTGGCTT CTGAATTTCC TGCTAATAAC 
AAGGAGCTTT CATTGGAAAT GAATACAATT TTGCGCCGCG GTTCATCTAA CGAAAATATC 
AAACAACAAA AACAGCAGAT AACCGAATAT TTCAATACCA ATATTGTTCA GTACAGAACA 
ACTTCATCGT CTAAAATCAT GTTTTTAGCG GCTGCCGTTC TTCTAGAAAC GATAAGATGC 
GAGGCAGGTG ATTGTTCGAA GACTCTACTG TACTTCTCAG ACCCCTCCAT TCTTTCCGGT 
TCAATTGAGA AGTGTATTGC AGTTTTGTCG GTTTCCATGA TTAGAAAATA TGCTAGGTTG 
ATTCAAAAGG GTAACGATGC CATATTCAAC TCCAAAATGA TTGCTCAACA ACTTAATAAC 
TTGCTACTTT GCCTTTCTCA TAGGGAACCA ACTTTGCAGG ATGCCGCTTT TCATGCCTGT 
GAAATATTTA TCAGATCTAT TCCATCATCG TTATGTCATC ATTTGTCTCT CTACACCTTA 
TTAGATATGT TAACAGCCCT ATTTGATAGT ATCTTGGACT CAGAGGCGCA TAAATTTGAA 
CCACGGTATG AATTTAAATT AAAACATTCT AAGACAACAA TATTGGTTCC AAGCTCATCG 
TCCTGGCGCG CTACGACACT ATCAAGATTG CACAAGTCAG CTAAAGAATG GGTAAGAATT 
CTATTGAATA GAAGCAACCA GGATACTAAG ATTTTGTTGC AATCATACAT ATCAGATCTC 
GGTGAGTACA GCAGGCTAAA TTCTGTTGAA TTTGGTGTCT CATTTGCCAT GGATATGGCT 
GGTTTGATTT TACCTGCAGA TAAGGAATTA TCAAGGCTTA CTTATTATGG TCCAGAGAAA 
CCTAACACGA TTTCTGGATT TATATCTTTA CATTCTTGGA GGTCAAAATA TCTTTTCGAT 
ACCGCTATTA CATCATCACC AGAGGATATC AAAAGGCAAA TAGGTATTTC CACTCAAAAC 
ATAAGAAAAA ATTTGACCTT AGGAAATAAG ATTATAACTA AAGACGTAAC TGATTTTCTT 
GATATGGCTA CCGCGTTGTT AATTCTTGGC AATGGTGCAC CAGCGTCATT GATATATGAT 
ATTGTGCACA TCCCGTTCGA AGTCTTCACC TCTGCATCTT TAAAGATTGC TACAAACGTA 
TGGTTAACAA TCATAACTGA GAAGCCCGAA GTTGCACATT TGCTTTTAGT TGAAGTATGC 
TATTGCTGGA TGCGCTCCAT TGATGACAAT ATTGGTCTCT ATTCTCGCGA TCATGACTTA 
AAGGGCGAAG AATACCAAAA AATGGAATAT TCCCCTTACG ACAAAGCAGG TATCAACAGA 
GATGCAAAAA ATGCATCCCA AGCTATGCAA CCACATCTTC ATGTTATTAA ATTTTTTGCT 
TCCCATTTCG AGGGCACACT ATTTCAAAGT GACTTTTTAT TGAAAATTTT CACGAAATGT 
GCGCTTTATG GTATCAAGAA TCTGTATAAG GCTTCACTAC ACCCGTTCGC TAGAATGATT 
CGCCACGAAT TATTGTTATT CGCGACTCTC GTACTAAACG CAAGTTATAA GCAGGGATCC 
AAGTATATGG GCCGTTTGTC GCAAGAAATC ACAAACGGTG CCCTAAGTTG GTTTAAAAGA 
CCAGTAGCGT GGCCATTCGG CTCAAATGAG CTGAAAATCA AAGCAGATTT ATCTGTTACT 
AGGGACCTTT TCCTTCAGCT CAACAAATTA AGCTCGCTAA TGTCACGTCA TTGCGGGAAA 
GATTACAAGA TCCTGAACTA TTTCTTGGCA AGCGAAATCC AGCAGATTCA AACCTGGCTT 
ACTCCGACTG AGAAGATTGA AGGAGCCGAC AGCAACGAGC TTACAAGCGA TATCGTTGAA 
GCTACCTTTG CTAAAGATCC AACATTAGCA ATAAATCTCT TACAACGATG TTATAGCAAG 
AAAGCTGAGG ATGTTTTAGT GGGCTTAGTT GCAAAACATG CTTTAATGTG TGTGGGGTCC 
CCAAGTGCTC TTGACCTGTT CATAAAAGGA AGCCACCTGA GCAGTAAGAA AGACCTACAC 
GCAACCTTAT ACTGGGCGCC AGTGAGCCCG TTAAAATCTA TCAACCTTTT CCTTCCCGAA 
TGGCAAGGTA ATTCTTTTAT CTTACAATTC AGCATATATT CGTTGGAATC ACAAGATGTG 
AACTTGGCAT TCTTCTATGT TCCTCAAATC GTACAATGTT TGAGATACGA TAAAACCGGA 
TATGTCGAAA GATTGATTTT GGATACTGCG AAAATTAGTG TGTTATTTTC TCATCAAATA 
ATCTGGAATA TGCTTGCAAA CTGCTACAAG GATGATGAAG GTATACAAGA AGATGAAATC 
AAACCAACTC TAGATCGTAT TAGGGAGCGT ATGGTTTCAA GTTTCAGCCA ATCTCATCGC 
GATTTTTACG AACGTGAATT TGAATTCTTC GACGAAGTAA CTGGCATATC TGGTAAGTTG 
AAACCATACA TAAAAAAAAG TAAGGCTGAA AAGAAACATA AGATCGATGA AGAAATGAGC 
AAAATTGAGG TGAAACCTGA TGTTTATTTA CCTTCTAATC CTGACGGTGT AGTTATTGAT 
ATTGATCGGA AGAGTGGTAA GCCACTTCAA TCTCACGCAA AGGCGCCTTT TATGGCGACC 
TTTAAAATAA AGAAAGACGT AAAAGATCCT TTGACAGGTA AAAACAAGGA AGTTGAAAAA 
TGGCAAGCTG CTATCTTCAA AGTCGGTGAT GACTGTAGGC AAGATGTTCT AGCGTTACAA 
TTGATCTCGC TATTTAGAAC CATTTGGTCT AGTATTGGCT TGGATGTCTA CGTTTTTCCC 
TACAGAGTTA CTGCGACGGC ACCGGGTTGT GGTGTCATCG ATGTGCTACC CAATTCGGTA 
TCCCGTGATA TGTTAGGACG TGAAGCTGTT AATGGATTAT ATGAATATTT CACTAGTAAA 
TTTGGTAATG AATCTACTAT CGAATTTCAA AACGCACGAA ACAACTTTGT TAAATCCTTA 
GCGGGATATA GCGTAATTTC GTATTTGTTG CAATTCAAGG ATAGACATAA TGGTAACATT 
ATGTACGATG ATCAAGGACA TTGTCTACAT ATCGATTTTG GGTTTATTTT TGATATTGTC 
CCAGGTGGTA TCAAGTTTGA AGCAGTACCA TTCAAGCTGA CGAAAGAAAT GGTTAAAGTG 
ATGGGAGGTT CGCCCCAGAC CCCAGCGTAT CTGGACTTTG AAGAACTTTG TATCAAGGCA 
TATCTAGCCG CCCGTCCGCA CGTGGAGGCC ATAATTGAGT GTGTAAATCC TATGTTAGGA 
AGCGGTCTCC CCTGCTTTAA GGGTCACAAG ACAATTAGGA ATCTAAGAGC AAGATTTCAA 
CCTCAAAAAA CCGATCACGA AGCTGCACTA TATATGAAGG CGCTAATCCG TAAAAGTTAT 
GAAAGTATAT TCACTAAAGG TTATGATGAA TTCCAAAGGC TCACAAATGG CATTCCGTAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_013408.1
CDS	1..5703
Translation	MRFTRGLKASSSLRAKAIGRLTKLSTGAPNDQNSNGTTLDLITHTLPIFYSTNTSKIYTIPLTLSEWEVLTSLCVAIPTTLDLVETMLKEIIAPYFLETPRQRISDVLSSKFKLEQMRNPIELLTFQLTKFMIQACEQYPVLYENIGGIISTYFERVLKIFTIKQSGLLSLVGFINAFIQFPNSTELTKFTWKKLAKLVLRGSFLNEVDKILNSSATFTNDSIVQYYDAGNELSSAYLLELISRLQVSLISHLLNTSHVGANLSEFLLNQQYQFYKFDQEVADENDDTKCIDDFFFNVRSNKQFFTDMCKISLQFCSESHILDLSTDNRARFSFDTRAHYLQTLCLIPFIEDTESELFESFTNVVSESIDKFFLSDVVTPSLIKAIVASASLLNFFTEKLSLTLIRMFPLLVASPHITTETVNDVAKIFTTGLYPLNEDAIVSTIYSMNNLLAVSEDGSPVPVLRERQLTITSGKNIEKDYFPLRNSSASLDGTGALLGNTTVGQLSSHDVNSGATMTYHASLISNCVAATTTIASYYNTQSITALTISILTQKVNSMSKELDGVILNSLARLAPNTSLTEFSLLLKFFKSRTVIATKIDDSALLKNIIKAKCVISKELLARHFSSDLYFMYLHDLLDSIIASGEVERLEHHRPQTEISRVADQIATYLEPLAALLPVPGDTPLDINKDEVTTNKFRNAWFNFVIHGYHLGGPIVKRNFSFLLTIAYNSPPLASEFPANNKELSLEMNTILRRGSSNENIKQQKQQITEYFNTNIVQYRTTSSSKIMFLAAAVLLETIRCEAGDCSKTLLYFSDPSILSGSIEKCIAVLSVSMIRKYARLIQKGNDAIFNSKMIAQQLNNLLLCLSHREPTLQDAAFHACEIFIRSIPSSLCHHLSLYTLLDMLTALFDSILDSEAHKFEPRYEFKLKHSKTTILVPSSSSWRATTLSRLHKSAKEWVRILLNRSNQDTKILLQSYISDLGEYSRLNSVEFGVSFAMDMAGLILPADKELSRLTYYGPEKPNTISGFISLHSWRSKYLFDTAITSSPEDIKRQIGISTQNIRKNLTLGNKIITKDVTDFLDMATALLILGNGAPASLIYDIVHIPFEVFTSASLKIATNVWLTIITEKPEVAHLLLVEVCYCWMRSIDDNIGLYSRDHDLKGEEYQKMEYSPYDKAGINRDAKNASQAMQPHLHVIKFFASHFEGTLFQSDFLLKIFTKCALYGIKNLYKASLHPFARMIRHELLLFATLVLNASYKQGSKYMGRLSQEITNGALSWFKRPVAWPFGSNELKIKADLSVTRDLFLQLNKLSSLMSRHCGKDYKILNYFLASEIQQIQTWLTPTEKIEGADSNELTSDIVEATFAKDPTLAINLLQRCYSKKAEDVLVGLVAKHALMCVGSPSALDLFIKGSHLSSKKDLHATLYWAPVSPLKSINLFLPEWQGNSFILQFSIYSLESQDVNLAFFYVPQIVQCLRYDKTGYVERLILDTAKISVLFSHQIIWNMLANCYKDDEGIQEDEIKPTLDRIRERMVSSFSQSHRDFYEREFEFFDEVTGISGKLKPYIKKSKAEKKHKIDEEMSKIEVKPDVYLPSNPDGVVIDIDRKSGKPLQSHAKAPFMATFKIKKDVKDPLTGKNKEVEKWQAAIFKVGDDCRQDVLALQLISLFRTIWSSIGLDVYVFPYRVTATAPGCGVIDVLPNSVSRDMLGREAVNGLYEYFTSKFGNESTIEFQNARNNFVKSLAGYSVISYLLQFKDRHNGNIMYDDQGHCLHIDFGFIFDIVPGGIKFEAVPFKLTKEMVKVMGGSPQTPAYLDFEELCIKAYLAARPHVEAIIECVNPMLGSGLPCFKGHKTIRNLRARFQPQKTDHEAALYMKALIRKSYESIFTKGYDEFQRLTNGIPY

Target ORF information:

RefSeq Version	NM_001182193.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C 1-phosphatidylinositol 4-kinase STT4 (STT4), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001182193.1

ORF Insert Sequence:

ATGAGATTTA CCAGAGGATT GAAAGCCTCT TCATCTTTAA GAGCGAAAGC TATTGGAAGA 
CTAACTAAAC TTTCGACGGG TGCACCAAAT GATCAAAACA GTAATGGTAC AACATTAGAT 
TTGATAACTC ATACATTGCC TATTTTTTAT TCCACAAATA CGTCAAAGAT ATATACTATA 
CCACTTACAT TGAGTGAATG GGAAGTACTA ACTTCGCTTT GTGTTGCAAT CCCTACCACG 
CTCGATCTGG TAGAGACAAT GCTTAAAGAA ATAATTGCGC CTTATTTTCT GGAAACTCCA 
AGACAAAGAA TTTCAGATGT TCTGTCCTCG AAATTCAAGT TGGAACAGAT GAGAAATCCG 
ATTGAATTGC TAACTTTCCA GCTGACTAAG TTTATGATTC AAGCTTGTGA ACAGTATCCC 
GTTCTTTATG AAAATATAGG CGGCATCATT TCAACTTATT TTGAGCGTGT ATTAAAGATT 
TTTACTATTA AACAATCTGG TCTACTATCA TTAGTGGGCT TTATTAATGC ATTTATCCAG 
TTTCCCAACT CTACAGAGCT TACTAAATTC ACCTGGAAAA AGTTAGCGAA ACTTGTGCTT 
CGTGGATCAT TTCTAAACGA AGTTGATAAG ATTTTGAATT CCTCAGCAAC GTTTACCAAT 
GATTCAATCG TCCAGTATTA CGATGCTGGA AATGAGCTAT CCAGTGCCTA CTTATTGGAA 
TTAATATCTC GCTTGCAAGT TTCGCTAATA TCTCATCTGT TAAACACTTC ACATGTCGGC 
GCCAACTTGA GTGAGTTCTT ACTAAATCAA CAATACCAAT TTTACAAATT TGATCAAGAA 
GTAGCCGATG AAAACGATGA TACGAAGTGT ATTGATGACT TTTTTTTCAA CGTAAGAAGC 
AATAAACAGT TTTTTACAGA CATGTGTAAA ATTTCCCTGC AATTTTGTTC TGAGTCACAT 
ATTCTCGACC TATCTACAGA CAACCGTGCA AGATTTTCTT TTGATACTCG AGCCCACTAC 
CTACAAACGC TATGTTTAAT TCCGTTTATA GAAGATACAG AAAGCGAGCT TTTTGAAAGT 
TTTACAAACG TTGTTTCTGA ATCTATTGAT AAATTTTTCT TATCTGATGT CGTTACACCA 
TCTTTGATAA AAGCAATTGT AGCTTCTGCC TCATTACTGA ATTTTTTCAC GGAAAAATTG 
TCATTAACTC TGATTCGTAT GTTTCCTTTA TTGGTAGCGT CTCCACATAT CACGACTGAA 
ACAGTTAATG ATGTTGCAAA AATATTCACC ACAGGGTTAT ACCCTCTAAA TGAAGATGCA 
ATTGTGAGTA CAATTTATTC CATGAACAAC TTATTAGCTG TTTCCGAGGA CGGATCGCCC 
GTCCCGGTGC TCCGTGAGCG CCAATTAACA ATAACATCTG GAAAAAATAT TGAGAAAGAC 
TACTTTCCCT TGCGAAATTC ATCTGCCAGT TTGGATGGCA CTGGTGCTCT GCTCGGAAAT 
ACAACTGTGG GCCAACTTTC CTCCCATGAT GTCAATAGTG GAGCTACTAT GACATACCAT 
GCCTCTTTGA TATCTAATTG TGTGGCTGCA ACGACTACAA TCGCCTCATA CTATAACACC 
CAAAGCATAA CAGCTTTGAC TATCTCTATT CTTACGCAAA AAGTTAATTC TATGTCAAAG 
GAATTAGACG GTGTTATTTT GAATTCTTTG GCAAGACTGG CTCCGAATAC TTCACTGACA 
GAATTTTCGC TTTTACTAAA GTTCTTCAAA TCGAGAACTG TTATTGCAAC AAAAATTGAT 
GATAGCGCAC TATTGAAAAA TATTATTAAA GCGAAATGTG TGATATCAAA AGAATTGTTG 
GCCAGGCATT TTTCAAGTGA CTTATATTTC ATGTATCTTC ATGATCTATT GGACTCAATC 
ATTGCCAGTG GGGAAGTAGA ACGATTGGAA CATCACAGAC CTCAAACGGA GATTTCCCGT 
GTTGCTGATC AAATCGCTAC CTACTTAGAG CCCCTCGCCG CTCTACTACC TGTTCCAGGC 
GATACGCCAT TGGACATCAA TAAGGATGAA GTCACTACAA ACAAGTTTAG GAACGCCTGG 
TTTAATTTTG TCATCCATGG GTACCACTTG GGCGGGCCTA TTGTCAAACG AAATTTTTCT 
TTCTTATTAA CTATTGCCTA TAATTCACCA CCGCTGGCTT CTGAATTTCC TGCTAATAAC 
AAGGAGCTTT CATTGGAAAT GAATACAATT TTGCGCCGCG GTTCATCTAA CGAAAATATC 
AAACAACAAA AACAGCAGAT AACCGAATAT TTCAATACCA ATATTGTTCA GTACAGAACA 
ACTTCATCGT CTAAAATCAT GTTTTTAGCG GCTGCCGTTC TTCTAGAAAC GATAAGATGC 
GAGGCAGGTG ATTGTTCGAA GACTCTACTG TACTTCTCAG ACCCCTCCAT TCTTTCCGGT 
TCAATTGAGA AGTGTATTGC AGTTTTGTCG GTTTCCATGA TTAGAAAATA TGCTAGGTTG 
ATTCAAAAGG GTAACGATGC CATATTCAAC TCCAAAATGA TTGCTCAACA ACTTAATAAC 
TTGCTACTTT GCCTTTCTCA TAGGGAACCA ACTTTGCAGG ATGCCGCTTT TCATGCCTGT 
GAAATATTTA TCAGATCTAT TCCATCATCG TTATGTCATC ATTTGTCTCT CTACACCTTA 
TTAGATATGT TAACAGCCCT ATTTGATAGT ATCTTGGACT CAGAGGCGCA TAAATTTGAA 
CCACGGTATG AATTTAAATT AAAACATTCT AAGACAACAA TATTGGTTCC AAGCTCATCG 
TCCTGGCGCG CTACGACACT ATCAAGATTG CACAAGTCAG CTAAAGAATG GGTAAGAATT 
CTATTGAATA GAAGCAACCA GGATACTAAG ATTTTGTTGC AATCATACAT ATCAGATCTC 
GGTGAGTACA GCAGGCTAAA TTCTGTTGAA TTTGGTGTCT CATTTGCCAT GGATATGGCT 
GGTTTGATTT TACCTGCAGA TAAGGAATTA TCAAGGCTTA CTTATTATGG TCCAGAGAAA 
CCTAACACGA TTTCTGGATT TATATCTTTA CATTCTTGGA GGTCAAAATA TCTTTTCGAT 
ACCGCTATTA CATCATCACC AGAGGATATC AAAAGGCAAA TAGGTATTTC CACTCAAAAC 
ATAAGAAAAA ATTTGACCTT AGGAAATAAG ATTATAACTA AAGACGTAAC TGATTTTCTT 
GATATGGCTA CCGCGTTGTT AATTCTTGGC AATGGTGCAC CAGCGTCATT GATATATGAT 
ATTGTGCACA TCCCGTTCGA AGTCTTCACC TCTGCATCTT TAAAGATTGC TACAAACGTA 
TGGTTAACAA TCATAACTGA GAAGCCCGAA GTTGCACATT TGCTTTTAGT TGAAGTATGC 
TATTGCTGGA TGCGCTCCAT TGATGACAAT ATTGGTCTCT ATTCTCGCGA TCATGACTTA 
AAGGGCGAAG AATACCAAAA AATGGAATAT TCCCCTTACG ACAAAGCAGG TATCAACAGA 
GATGCAAAAA ATGCATCCCA AGCTATGCAA CCACATCTTC ATGTTATTAA ATTTTTTGCT 
TCCCATTTCG AGGGCACACT ATTTCAAAGT GACTTTTTAT TGAAAATTTT CACGAAATGT 
GCGCTTTATG GTATCAAGAA TCTGTATAAG GCTTCACTAC ACCCGTTCGC TAGAATGATT 
CGCCACGAAT TATTGTTATT CGCGACTCTC GTACTAAACG CAAGTTATAA GCAGGGATCC 
AAGTATATGG GCCGTTTGTC GCAAGAAATC ACAAACGGTG CCCTAAGTTG GTTTAAAAGA 
CCAGTAGCGT GGCCATTCGG CTCAAATGAG CTGAAAATCA AAGCAGATTT ATCTGTTACT 
AGGGACCTTT TCCTTCAGCT CAACAAATTA AGCTCGCTAA TGTCACGTCA TTGCGGGAAA 
GATTACAAGA TCCTGAACTA TTTCTTGGCA AGCGAAATCC AGCAGATTCA AACCTGGCTT 
ACTCCGACTG AGAAGATTGA AGGAGCCGAC AGCAACGAGC TTACAAGCGA TATCGTTGAA 
GCTACCTTTG CTAAAGATCC AACATTAGCA ATAAATCTCT TACAACGATG TTATAGCAAG 
AAAGCTGAGG ATGTTTTAGT GGGCTTAGTT GCAAAACATG CTTTAATGTG TGTGGGGTCC 
CCAAGTGCTC TTGACCTGTT CATAAAAGGA AGCCACCTGA GCAGTAAGAA AGACCTACAC 
GCAACCTTAT ACTGGGCGCC AGTGAGCCCG TTAAAATCTA TCAACCTTTT CCTTCCCGAA 
TGGCAAGGTA ATTCTTTTAT CTTACAATTC AGCATATATT CGTTGGAATC ACAAGATGTG 
AACTTGGCAT TCTTCTATGT TCCTCAAATC GTACAATGTT TGAGATACGA TAAAACCGGA 
TATGTCGAAA GATTGATTTT GGATACTGCG AAAATTAGTG TGTTATTTTC TCATCAAATA 
ATCTGGAATA TGCTTGCAAA CTGCTACAAG GATGATGAAG GTATACAAGA AGATGAAATC 
AAACCAACTC TAGATCGTAT TAGGGAGCGT ATGGTTTCAA GTTTCAGCCA ATCTCATCGC 
GATTTTTACG AACGTGAATT TGAATTCTTC GACGAAGTAA CTGGCATATC TGGTAAGTTG 
AAACCATACA TAAAAAAAAG TAAGGCTGAA AAGAAACATA AGATCGATGA AGAAATGAGC 
AAAATTGAGG TGAAACCTGA TGTTTATTTA CCTTCTAATC CTGACGGTGT AGTTATTGAT 
ATTGATCGGA AGAGTGGTAA GCCACTTCAA TCTCACGCAA AGGCGCCTTT TATGGCGACC 
TTTAAAATAA AGAAAGACGT AAAAGATCCT TTGACAGGTA AAAACAAGGA AGTTGAAAAA 
TGGCAAGCTG CTATCTTCAA AGTCGGTGAT GACTGTAGGC AAGATGTTCT AGCGTTACAA 
TTGATCTCGC TATTTAGAAC CATTTGGTCT AGTATTGGCT TGGATGTCTA CGTTTTTCCC 
TACAGAGTTA CTGCGACGGC ACCGGGTTGT GGTGTCATCG ATGTGCTACC CAATTCGGTA 
TCCCGTGATA TGTTAGGACG TGAAGCTGTT AATGGATTAT ATGAATATTT CACTAGTAAA 
TTTGGTAATG AATCTACTAT CGAATTTCAA AACGCACGAA ACAACTTTGT TAAATCCTTA 
GCGGGATATA GCGTAATTTC GTATTTGTTG CAATTCAAGG ATAGACATAA TGGTAACATT 
ATGTACGATG ATCAAGGACA TTGTCTACAT ATCGATTTTG GGTTTATTTT TGATATTGTC 
CCAGGTGGTA TCAAGTTTGA AGCAGTACCA TTCAAGCTGA CGAAAGAAAT GGTTAAAGTG 
ATGGGAGGTT CGCCCCAGAC CCCAGCGTAT CTGGACTTTG AAGAACTTTG TATCAAGGCA 
TATCTAGCCG CCCGTCCGCA CGTGGAGGCC ATAATTGAGT GTGTAAATCC TATGTTAGGA 
AGCGGTCTCC CCTGCTTTAA GGGTCACAAG ACAATTAGGA ATCTAAGAGC AAGATTTCAA 
CCTCAAAAAA CCGATCACGA AGCTGCACTA TATATGAAGG CGCTAATCCG TAAAAGTTAT 
GAAAGTATAT TCACTAAAGG TTATGATGAA TTCCAAAGGC TCACAAATGG CATTCCGTAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XII.
Nature387(6632 Suppl)87-90(1997 May)
Johnston M,Hillier L,Riles L,Albermann K,Andr? B,Ansorge W,Benes V,Br?ckner M,Delius H,Dubois E,D?sterh?ft A,Entian KD,Floeth M,Goffeau A,Hebling U,Heumann K,Heuss-Neitzel D,Hilbert H,Hilger F,Kleine K,K?tter P,Louis EJ,Messenguy F,Mewes HW,Hoheisel JD

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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STT4 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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