MCM5 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	MCM5
Entrez Gene ID	850980
Full Name	MCM DNA helicase complex subunit MCM5
Synonyms	BOB1,CDC46
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001182161.1	NP_013376.1	MCM DNA helicase complex subunit MCM5

Pathways from BioSystems

Homologies

Homo sapiens (human)	MCM5	NP_006730.2
Rattus norvegicus (Norway rat)	Mcm5	NP_001099640.1
Oryza sativa (rice)	Os02g0797400	NP_001048396.1
Pan troglodytes (chimpanzee)	MCM5	XP_001156025.1
Canis lupus familiaris (dog)	MCM5	XP_005626282.1
Caenorhabditis elegans (roundworm)	mcm-5	NP_497858.1
Saccharomyces cerevisiae (baker's yeast)	MCM5	NP_013376.1
Neurospora crassa	NCU01171	XP_961537.1
Xenopus tropicalis (tropical clawed frog)	mcm5	NP_001017327.2
Bos taurus (cattle)	MCM5	NP_001068758.1
Mus musculus (house mouse)	Mcm5	NP_032592.2
Gallus gallus (chicken)	MCM5	NP_001006243.1
Drosophila melanogaster (fruit fly)	Mcm5	NP_524308.2
Kluyveromyces lactis	KLLA0D09262g	XP_453475.1
Schizosaccharomyces pombe (fission yeast)	mcm5	XP_001713071.1
Danio rerio (zebrafish)	mcm5	NP_848523.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004408	XP_313694.2
Eremothecium gossypii	AGOS_AGR276W	NP_986942.1
Magnaporthe oryzae (rice blast fungus)	MGG_08646	XP_003711077.1
Arabidopsis thaliana (thale cress)	MCM5	NP_178812.1

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XII.
Johnston M, Hillier L, Riles L, Albermann K, Andr? B, Ansorge W, Benes V, Br?ckner M, Delius H, Dubois E, D?sterh?ft A, Entian KD, Floeth M, Goffeau A, Hebling U, Heumann K, Heuss-Neitzel D, Hilbert H, Hilger F, Kleine K, K?tter P, Louis EJ, Messenguy F, Mewes HW, Hoheisel JD
Nature387(6632 Suppl)87-90(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The Mcm ATPase active sites that flank Mcm2/5 (i.e. Mcm5/3 and 6/2) modulate the biochemical activities of the putative Mcm2/5 gate.
Title: The Saccharomyces cerevisiae Mcm6/2 and Mcm5/3 ATPase active sites contribute to the function of the putative Mcm2-7 'gate'.

DNA runs through a central channel in the double hexamer, and, once loaded, Mcm2-7 can slide passively along double-stranded DNA.
Title: Concerted loading of Mcm2-7 double hexamers around DNA during DNA replication origin licensing.

Conclusions predict a structural discontinuity between Mcm2 and Mcm5 and demonstrate that in contrast to other hexameric helicases, the six Mcm2-7 active sites are functionally distinct.
Title: Subunit organization of Mcm2-7 and the unequal role of active sites in ATP hydrolysis and viability.

Mcm5 protein has a positive role in origin binding, which requires coordination by all six Mcm2-7 subunits in the hexamer.
Title: Functional conservation of beta-hairpin DNA binding domains in the Mcm protein of Methanobacterium thermoautotrophicum and the Mcm5 protein of Saccharomyces cerevisiae.

A domain-push model is proposed to explain the bypass activity of mutant proline62leucine BOB1, which has a major effect on helicase activity, but no significant impact on DNA binding or ATPase of Methanobacterium thermoautotrophicum MCM gene.
Title: Biochemical activities of the BOB1 mutant in Methanobacterium thermoautotrophicum MCM.

The following MCM5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MCM5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi00605	NM_001182161.1 Latest version!	Saccharomyces cerevisiae S288C MCM DNA helicase complex subunit MCM5 (MCM5), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MCM5 ( NM_001182161.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_013376.1 Saccharomyces cerevisiae S288C MCM DNA helicase complex subunit MCM5 (MCM5), partial mRNA.

Price & Availability↑

CloneID	OSi00605
Clone ID Related Accession (Same CDS sequence)	NM_001182161.1
Accession Version	NM_001182161.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2328bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	MCM DNA helicase complex subunit MCM5
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001144). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGTCATTTG ATAGACCGGA AATATACAGT GCTCCTGTTT TACAAGGAGA ATCTCCTAAC 
GACGATGATA ATACTGAAAT CATAAAGTCC TTTAAGAATT TCATTTTGGA GTTCAGACTT 
GACTCGCAAT TTATTTACAG AGATCAGTTA AGGAACAACA TCCTTGTGAA GAATTATTCT 
TTAACGGTTA ACATGGAGCA TTTGATCGGA TATAACGAAG ACATATATAA GAAACTATCA 
GACGAACCTT CAGATATCAT TCCATTATTC GAAACCGCGA TCACACAAGT GGCTAAAAGG 
ATAAGTATTC TAAGCAGAGC TCAATCTGCT AATAACAATG ACAAAGATCC AGAAAATACT 
AGTATGGATA CTGATTCTCT CTTATTGAAC TCTTTACCAA CATTTCAATT AATTTTAAAC 
TCCAATGCAA ATCAGATTCC ATTGAGAGAT TTGGATTCTG AACACGTCTC CAAGATTGTC 
CGTTTATCAG GTATTATAAT ATCCACGTCA GTTTTATCTT CCCGTGCCAC GTACCTTTCT 
ATAATGTGCA GAAATTGCAG ACACACAACA TCAATAACAA TCAACAATTT CAATTCTATC 
ACAGGCAATA CCGTCAGTTT ACCACGTTCT TGCTTATCTA CGATTGAGAG TGAATCTTCT 
ATGGCAAACG AGTCGAATAT TGGTGATGAA TCGACCAAGA AAAATTGTGG ACCTGATCCA 
TATATTATTA TCCATGAGTC TTCAAAGTTT ATTGATCAAC AGTTTTTAAA ATTACAGGAA 
ATCCCAGAAC TGGTTCCAGT AGGTGAGATG CCTAGAAACT TAACAATGAC TTGTGACCGA 
TACCTAACAA ACAAAGTTAT TCCTGGTACG AGAGTCACTA TAGTAGGTAT TTATTCCATC 
TATAATTCTA AAAATGGTGC CGGATCCGGA AGGAGCGGGG GTGGAAATGG AGGAAGTGGT 
GTTGCTATTA GAACACCTTA TATCAAAATA TTAGGTATTC AGTCCGATGT AGAAACCTCC 
TCTATTTGGA ATTCAGTAAC TATGTTTACT GAGGAGGAAG AAGAGGAATT TCTACAGCTA 
AGTAGAAACC CGAAGCTTTA TGAAATTTTG ACCAACTCTA TTGCCCCCTC TATTTTTGGT 
AATGAAGATA TAAAAAAAGC CATTGTATGT TTATTGATGG GTGGTTCCAA GAAGATATTA 
CCCGATGGTA TGAGGTTAAG AGGTGATATC AATGTACTAT TATTAGGTGA TCCAGGTACC 
GCCAAATCTC AACTATTGAA ATTTGTGGAG AAAGTGTCAC CTATTGCGGT ATATACATCT 
GGTAAGGGAT CTTCTGCAGC TGGGTTAACT GCCAGTGTAC AAAGAGATCC GATGACAAGA 
GAATTTTATT TGGAAGGTGG TGCTATGGTG CTTGCCGATG GTGGTGTTGT ATGCATCGAT 
GAATTCGATA AAATGAGAGA TGAAGATAGA GTGGCCATTC ATGAAGCTAT GGAGCAGCAA 
ACAATCTCCA TCGCAAAAGC TGGTATCACT ACAGTGCTAA ATTCTAGAAC TAGTGTTTTA 
GCGGCTGCTA ATCCGATATA CGGCCGGTAT GATGATTTGA AGTCTCCTGG TGACAACATT 
GATTTCCAAA CTACTATTTT ATCCCGTTTT GATATGATTT TTATTGTTAA GGATGACCAT 
AATGAAGAAC GTGATATTTC AATAGCTAAC CACGTTATTA ATATTCATAC AGGAAATGCT 
AATGCTATGC AAAACCAACA AGAGGAAAAT GGCAGTGAAA TTAGTATTGA AAAGATGAAA 
CGTTACATTA CGTATTGTAG ATTGAAATGT GCACCAAGAC TTTCACCGCA GGCCGCTGAA 
AAACTGTCAT CGAACTTCGT CACCATTAGG AAGCAATTAT TAATCAACGA ATTAGAGTCA 
ACGGAAAGGT CGTCTATTCC AATTACCATT CGTCAATTAG AAGCTATTAT TAGAATAACA 
GAATCATTAG CCAAGTTAGA ATTAAGTCCT ATTGCACAGG AGAGACATGT TGACGAAGCT 
ATTAGATTGT TTCAAGCTTC CACAATGGAC GCAGCGTCTC AGGATCCAAT TGGCGGCTTA 
AATCAAGCAA GCGGAACATC TTTGTCAGAA ATCCGTCGTT TTGAACAAGA ACTAAAAAGA 
AGATTACCTA TAGGCTGGTC TACTTCTTAT CAAACTTTGA GGAGAGAATT TGTAGATACA 
CATAGATTTT CTCAATTAGC ACTGGATAAG GCCTTATATG CCCTAGAGAA GCATGAAACA 
ATTCAATTGA GACACCAGGG ACAGAATATT TACAGAAGTG GTGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_013376.1
CDS	1..2328
Translation	MSFDRPEIYSAPVLQGESPNDDDNTEIIKSFKNFILEFRLDSQFIYRDQLRNNILVKNYSLTVNMEHLIGYNEDIYKKLSDEPSDIIPLFETAITQVAKRISILSRAQSANNNDKDPENTSMDTDSLLLNSLPTFQLILNSNANQIPLRDLDSEHVSKIVRLSGIIISTSVLSSRATYLSIMCRNCRHTTSITINNFNSITGNTVSLPRSCLSTIESESSMANESNIGDESTKKNCGPDPYIIIHESSKFIDQQFLKLQEIPELVPVGEMPRNLTMTCDRYLTNKVIPGTRVTIVGIYSIYNSKNGAGSGRSGGGNGGSGVAIRTPYIKILGIQSDVETSSIWNSVTMFTEEEEEEFLQLSRNPKLYEILTNSIAPSIFGNEDIKKAIVCLLMGGSKKILPDGMRLRGDINVLLLGDPGTAKSQLLKFVEKVSPIAVYTSGKGSSAAGLTASVQRDPMTREFYLEGGAMVLADGGVVCIDEFDKMRDEDRVAIHEAMEQQTISIAKAGITTVLNSRTSVLAAANPIYGRYDDLKSPGDNIDFQTTILSRFDMIFIVKDDHNEERDISIANHVINIHTGNANAMQNQQEENGSEISIEKMKRYITYCRLKCAPRLSPQAAEKLSSNFVTIRKQLLINELESTERSSIPITIRQLEAIIRITESLAKLELSPIAQERHVDEAIRLFQASTMDAASQDPIGGLNQASGTSLSEIRRFEQELKRRLPIGWSTSYQTLRREFVDTHRFSQLALDKALYALEKHETIQLRHQGQNIYRSGV

Target ORF information:

RefSeq Version	NM_001182161.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C MCM DNA helicase complex subunit MCM5 (MCM5), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001182161.1

ORF Insert Sequence:

ATGTCATTTG ATAGACCGGA AATATACAGT GCTCCTGTTT TACAAGGAGA ATCTCCTAAC 
GACGATGATA ATACTGAAAT CATAAAGTCC TTTAAGAATT TCATTTTGGA GTTCAGACTT 
GACTCGCAAT TTATTTACAG AGATCAGTTA AGGAACAACA TCCTTGTGAA GAATTATTCT 
TTAACGGTTA ACATGGAGCA TTTGATCGGA TATAACGAAG ACATATATAA GAAACTATCA 
GACGAACCTT CAGATATCAT TCCATTATTC GAAACCGCGA TCACACAAGT GGCTAAAAGG 
ATAAGTATTC TAAGCAGAGC TCAATCTGCT AATAACAATG ACAAAGATCC AGAAAATACT 
AGTATGGATA CTGATTCTCT CTTATTGAAC TCTTTACCAA CATTTCAATT AATTTTAAAC 
TCCAATGCAA ATCAGATTCC ATTGAGAGAT TTGGATTCTG AACACGTCTC CAAGATTGTC 
CGTTTATCAG GTATTATAAT ATCCACGTCA GTTTTATCTT CCCGTGCCAC GTACCTTTCT 
ATAATGTGCA GAAATTGCAG ACACACAACA TCAATAACAA TCAACAATTT CAATTCTATC 
ACAGGCAATA CCGTCAGTTT ACCACGTTCT TGCTTATCTA CGATTGAGAG TGAATCTTCT 
ATGGCAAACG AGTCGAATAT TGGTGATGAA TCGACCAAGA AAAATTGTGG ACCTGATCCA 
TATATTATTA TCCATGAGTC TTCAAAGTTT ATTGATCAAC AGTTTTTAAA ATTACAGGAA 
ATCCCAGAAC TGGTTCCAGT AGGTGAGATG CCTAGAAACT TAACAATGAC TTGTGACCGA 
TACCTAACAA ACAAAGTTAT TCCTGGTACG AGAGTCACTA TAGTAGGTAT TTATTCCATC 
TATAATTCTA AAAATGGTGC CGGATCCGGA AGGAGCGGGG GTGGAAATGG AGGAAGTGGT 
GTTGCTATTA GAACACCTTA TATCAAAATA TTAGGTATTC AGTCCGATGT AGAAACCTCC 
TCTATTTGGA ATTCAGTAAC TATGTTTACT GAGGAGGAAG AAGAGGAATT TCTACAGCTA 
AGTAGAAACC CGAAGCTTTA TGAAATTTTG ACCAACTCTA TTGCCCCCTC TATTTTTGGT 
AATGAAGATA TAAAAAAAGC CATTGTATGT TTATTGATGG GTGGTTCCAA GAAGATATTA 
CCCGATGGTA TGAGGTTAAG AGGTGATATC AATGTACTAT TATTAGGTGA TCCAGGTACC 
GCCAAATCTC AACTATTGAA ATTTGTGGAG AAAGTGTCAC CTATTGCGGT ATATACATCT 
GGTAAGGGAT CTTCTGCAGC TGGGTTAACT GCCAGTGTAC AAAGAGATCC GATGACAAGA 
GAATTTTATT TGGAAGGTGG TGCTATGGTG CTTGCCGATG GTGGTGTTGT ATGCATCGAT 
GAATTCGATA AAATGAGAGA TGAAGATAGA GTGGCCATTC ATGAAGCTAT GGAGCAGCAA 
ACAATCTCCA TCGCAAAAGC TGGTATCACT ACAGTGCTAA ATTCTAGAAC TAGTGTTTTA 
GCGGCTGCTA ATCCGATATA CGGCCGGTAT GATGATTTGA AGTCTCCTGG TGACAACATT 
GATTTCCAAA CTACTATTTT ATCCCGTTTT GATATGATTT TTATTGTTAA GGATGACCAT 
AATGAAGAAC GTGATATTTC AATAGCTAAC CACGTTATTA ATATTCATAC AGGAAATGCT 
AATGCTATGC AAAACCAACA AGAGGAAAAT GGCAGTGAAA TTAGTATTGA AAAGATGAAA 
CGTTACATTA CGTATTGTAG ATTGAAATGT GCACCAAGAC TTTCACCGCA GGCCGCTGAA 
AAACTGTCAT CGAACTTCGT CACCATTAGG AAGCAATTAT TAATCAACGA ATTAGAGTCA 
ACGGAAAGGT CGTCTATTCC AATTACCATT CGTCAATTAG AAGCTATTAT TAGAATAACA 
GAATCATTAG CCAAGTTAGA ATTAAGTCCT ATTGCACAGG AGAGACATGT TGACGAAGCT 
ATTAGATTGT TTCAAGCTTC CACAATGGAC GCAGCGTCTC AGGATCCAAT TGGCGGCTTA 
AATCAAGCAA GCGGAACATC TTTGTCAGAA ATCCGTCGTT TTGAACAAGA ACTAAAAAGA 
AGATTACCTA TAGGCTGGTC TACTTCTTAT CAAACTTTGA GGAGAGAATT TGTAGATACA 
CATAGATTTT CTCAATTAGC ACTGGATAAG GCCTTATATG CCCTAGAGAA GCATGAAACA 
ATTCAATTGA GACACCAGGG ACAGAATATT TACAGAAGTG GTGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XII.
Nature387(6632 Suppl)87-90(1997 May)
Johnston M,Hillier L,Riles L,Albermann K,Andr? B,Ansorge W,Benes V,Br?ckner M,Delius H,Dubois E,D?sterh?ft A,Entian KD,Floeth M,Goffeau A,Hebling U,Heumann K,Heuss-Neitzel D,Hilbert H,Hilger F,Kleine K,K?tter P,Louis EJ,Messenguy F,Mewes HW,Hoheisel JD

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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MCM5 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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