NEJ1 cDNA ORF clone, Saccharomyces cerevisiae S288C

The nucleotide sequence of Saccharomyces cerevisiae chromosome XII.
Johnston M, Hillier L, Riles L, Albermann K, Andr? B, Ansorge W, Benes V, Br?ckner M, Delius H, Dubois E, D?sterh?ft A, Entian KD, Floeth M, Goffeau A, Hebling U, Heumann K, Heuss-Neitzel D, Hilbert H, Hilger F, Kleine K, K?tter P, Louis EJ, Messenguy F, Mewes HW, Hoheisel JD
Nature387(6632 Suppl)87-90(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

Our results reveal novel roles for Nej1 in the recruitment of Pol4 and Rad27 to in vivo DNA double strand breaks and the coordination of the end processing and ligation reactions of non-homologous end joining.
Title: Role of the yeast DNA repair protein Nej1 in end processing during the repair of DNA double strand breaks by non-homologous end joining.

C-terminus of Nej1 is critical for nuclear localization and non-homologous end-joining
Title: The C-terminus of Nej1 is critical for nuclear localization and non-homologous end-joining.

Nej1 not only plays an important role in determining repair pathway choice by participating in the initial NHEJ complex formed at DSBs but also contributes to the reactivation of Dnl4-Lif1 after repair is complete
Title: Yeast Nej1 is a key participant in the initial end binding and final ligation steps of nonhomologous end joining.

Nej1 physically interacts with Srs2.
Title: Nej1 recruits the Srs2 helicase to DNA double-strand breaks and supports repair by a single-strand annealing-like mechanism.

Structural characterization reveals that Nej1p exists as a dimer, and that residues 1-244 are sufficient for dimer formation
Title: A critical role for the C-terminus of Nej1 protein in Lif1p association, DNA binding and non-homologous end-joining.