EST1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	EST1
Entrez Gene ID	850934
Full Name	Est1p
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001182120.1	NP_013334.1	Est1p

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XII.
Johnston M, Hillier L, Riles L, Albermann K, Andr? B, Ansorge W, Benes V, Br?ckner M, Delius H, Dubois E, D?sterh?ft A, Entian KD, Floeth M, Goffeau A, Hebling U, Heumann K, Heuss-Neitzel D, Hilbert H, Hilger F, Kleine K, K?tter P, Louis EJ, Messenguy F, Mewes HW, Hoheisel JD
Nature387(6632 Suppl)87-90(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Recruitment of Est1 to telomeres via a Cdc13-Est1 fusion is sufficient to bypass the role of Ku in promoting telomere elongation.
Title: The principal role of Ku in telomere length maintenance is promotion of Est1 association with telomeres.

Autonomous localization of Est1p to the nucleus via the classical nuclear import pathway contributes to normal telomere length maintenance.
Title: Normal telomere length maintenance in Saccharomyces cerevisiae requires nuclear import of the ever shorter telomeres 1 (Est1) protein via the importin alpha pathway.

Esl1 has no telomere-related functions, but contributes to the regulation of adaptive gene expression responses.
Title: Yeast hEST1A/B (SMG5/6)-like proteins contribute to environment-sensing adaptive gene expression responses.

It is possible that the intramolecular G-quadruplex promoting activity of Est1 is biologically relevant in telomere replication in vivo.
Title: Characterization of the intramolecular G-quadruplex promoting activity of Est1.

There is a biochemical interaction between Est1 and the telomere binding protein Cdc13. Association of the Est1 protein with the telomerase catalytic core is not a regulatory event that contributes to telomere homeostasis.
Title: A yeast telomerase complex containing the Est1 recruitment protein is assembled early in the cell cycle.

The following EST1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EST1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi00568	NM_001182120.1 Latest version!	Saccharomyces cerevisiae S288C Est1p (EST1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

EST1 ( NM_001182120.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_013334.1 Saccharomyces cerevisiae S288C Est1p (EST1), partial mRNA.

Price & Availability↑

CloneID	OSi00568
Clone ID Related Accession (Same CDS sequence)	NM_001182120.1
Accession Version	NM_001182120.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2100bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	Est1p
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001144). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGGATAATG AAGAAGTTAA CGAAGAATGT ATGAGATTAT TTTTCAAGAA CGCTCGTGCG 
CATCTGGATA AACATCTAAC ATCAAGGTTG ACATGCGATG AAAATGCATA TATCACGTTC 
AGATGCTTCC TGGATGGAAT ACATCGCAAA TCTACTAGGT TTCTCGAAGA GCTACTTTTG 
AAACAAGAAA ATATGTACCA TAATAACAAT TACGAACGCA TAAATGATTC CGTGATACCA 
TTGGTTCTGA AACTTTTATG GCTTCAAATT CACGAACCTA CACTCCAATG GTTTGAGCAC 
TGGTTCCATG ATATCATGCG ACTAAGTAAC AGAAGAAAGT TCAGAGTTTT TAGAATTTTT 
CAAAAAAAAA TGATTCAATT TTTCAAAATT ACACACAGGT ATTACTATGA CATCATCGAA 
CACCTATGCG CAAAGTACGA TATGAATTCC GTTATTTCAA ATGCTCTCTT CGCGAAGTTG 
AATTTAATGC AATACACAGA TGGACTTTCA ACTCATGAGA AAATTATCTT AAACACGAGT 
AATCCACTGA CGTTTTCCAT TGTAATCTCA CTACAAAGAT GCGTGATTAA TCTAGGTTCC 
ACACATTTTT ATAAAACACT ACTAAACAAG CCGTCTAACA AACCCAAGAG TGTGGAAGGT 
TTTGAGAAGT CTATTAGGTA CTTGAATATT GCCTCACTCT ATCTCCCAGC CGTTGGAGAT 
ACTTATTTTC AACGAGCGAA AATTTACTTG ATCACTGGGA AATTCTCACT GTATTTCTTT 
GAATTAGTAA GAGGAGCATT GGTAAGGATT CCGTCTAAAT GTGCGTTAAA CAATTTGAAA 
GATTTCATTT TGACTCCTGA TTTTCCGGAA AGAAGACGTC TGATGAAAAA ATTGGCAATT 
CTTGTGTCAA AAGATCTCAA AGGTGAGAAA TCATTCTTTG AAGGTCAAAT TGTTTTGCAA 
TTTCTATCGA TAGTAGAACA CACTTTGGTT CCACAGTCAT GGAACGCATC ACGTGCTTCT 
AATTGTTGGT TATTGAAAGA GCATTTACAA ATGGCTGCAT TAAAGTATCA TTCAGGTAAT 
ATTAATGTTA TACTTGAAAA CTTGGCTGCC ACAATGGGAA GTTTCGATCT TATGTTTACA 
ACTCGAAAAA GTAAGGAACA AAAGAACAAA CTCAAATATG CAGATTTGAG TGAGCGCCAG 
GTTTTTTTTT TAGACTTGAG CTTTGATTTT ATTGCTAATA TCATAGACGT CGTCATCAAA 
CCCTCCTGGC AAAAAAACAT GGAAGACTTT CGATATCTAG CCATTATTCG TTTGCTTATG 
TGCTGGATTA AGTCATATAG ATCTATTTTG CAGTACACTC ACAGACACAG GAAGTTTTGC 
ACTTCATTCG CCTTGTTGCT GAACGACTTG ATAAATAGTC CACTGAATTG TTCAGGAAAT 
ATATATAGCC ACAGGCCGAA AAGAAGCTAT CTTTTTAGAG AAGATATTAT TTTCAGGGAA 
TTTTCTTGCA TTAACTTTGC ACTAACAGAT TTTAATGACG ATTATGTGTA TGATTCTCCC 
GACATGATTA ATAATATAAT TGGATGCCCT ACATTGACTA AAGTGCTTTC TCCAAAAGAA 
GAATGTGTTC TGCGAATTAG ATCAATAATA TTTTCTGGCA TGAAATTTTT AGAGAAAAAT 
GACACCGGCG TCATATGGAA TGCCAGCAAA TATAAGTTTG ATTTAATAAG CCCAAATATT 
AAAATAAAAC GCCAAATAGC ATTATCGGAA ATTTCCTCCA AAATAAATGT AAAAACACAA 
CAGGAAAGAG TAGTCTCTTC GAGAAAAGTT GAGGCCAAAA GAGATGAACA ACAGCGCAAA 
AGAGCCGGGA AAATAGCTGT GACAGAACTG GAAAAACAAT TTGCAAATGT CCGGAGAACA 
AAAAAATTGT CTCCGCTCCC AGAAAAAGAT GGCGTTTCTT CTGAGTTGGT AAAACATGCT 
GCTTCACGAG GGAGAAAAAC TATCACTGGC CCACTATCCT CTGATTTTCT CTCATATCCA 
GACGAAGCAA TTGATGCTGA TGAGGACATC ACCGTCCAAG TGCCAGATAC TCCTACTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_013334.1
CDS	1..2100
Translation	MDNEEVNEECMRLFFKNARAHLDKHLTSRLTCDENAYITFRCFLDGIHRKSTRFLEELLLKQENMYHNNNYERINDSVIPLVLKLLWLQIHEPTLQWFEHWFHDIMRLSNRRKFRVFRIFQKKMIQFFKITHRYYYDIIEHLCAKYDMNSVISNALFAKLNLMQYTDGLSTHEKIILNTSNPLTFSIVISLQRCVINLGSTHFYKTLLNKPSNKPKSVEGFEKSIRYLNIASLYLPAVGDTYFQRAKIYLITGKFSLYFFELVRGALVRIPSKCALNNLKDFILTPDFPERRRLMKKLAILVSKDLKGEKSFFEGQIVLQFLSIVEHTLVPQSWNASRASNCWLLKEHLQMAALKYHSGNINVILENLAATMGSFDLMFTTRKSKEQKNKLKYADLSERQVFFLDLSFDFIANIIDVVIKPSWQKNMEDFRYLAIIRLLMCWIKSYRSILQYTHRHRKFCTSFALLLNDLINSPLNCSGNIYSHRPKRSYLFREDIIFREFSCINFALTDFNDDYVYDSPDMINNIIGCPTLTKVLSPKEECVLRIRSIIFSGMKFLEKNDTGVIWNASKYKFDLISPNIKIKRQIALSEISSKINVKTQQERVVSSRKVEAKRDEQQRKRAGKIAVTELEKQFANVRRTKKLSPLPEKDGVSSELVKHAASRGRKTITGPLSSDFLSYPDEAIDADEDITVQVPDTPT

Target ORF information:

RefSeq Version	NM_001182120.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C Est1p (EST1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001182120.1

ORF Insert Sequence:

ATGGATAATG AAGAAGTTAA CGAAGAATGT ATGAGATTAT TTTTCAAGAA CGCTCGTGCG 
CATCTGGATA AACATCTAAC ATCAAGGTTG ACATGCGATG AAAATGCATA TATCACGTTC 
AGATGCTTCC TGGATGGAAT ACATCGCAAA TCTACTAGGT TTCTCGAAGA GCTACTTTTG 
AAACAAGAAA ATATGTACCA TAATAACAAT TACGAACGCA TAAATGATTC CGTGATACCA 
TTGGTTCTGA AACTTTTATG GCTTCAAATT CACGAACCTA CACTCCAATG GTTTGAGCAC 
TGGTTCCATG ATATCATGCG ACTAAGTAAC AGAAGAAAGT TCAGAGTTTT TAGAATTTTT 
CAAAAAAAAA TGATTCAATT TTTCAAAATT ACACACAGGT ATTACTATGA CATCATCGAA 
CACCTATGCG CAAAGTACGA TATGAATTCC GTTATTTCAA ATGCTCTCTT CGCGAAGTTG 
AATTTAATGC AATACACAGA TGGACTTTCA ACTCATGAGA AAATTATCTT AAACACGAGT 
AATCCACTGA CGTTTTCCAT TGTAATCTCA CTACAAAGAT GCGTGATTAA TCTAGGTTCC 
ACACATTTTT ATAAAACACT ACTAAACAAG CCGTCTAACA AACCCAAGAG TGTGGAAGGT 
TTTGAGAAGT CTATTAGGTA CTTGAATATT GCCTCACTCT ATCTCCCAGC CGTTGGAGAT 
ACTTATTTTC AACGAGCGAA AATTTACTTG ATCACTGGGA AATTCTCACT GTATTTCTTT 
GAATTAGTAA GAGGAGCATT GGTAAGGATT CCGTCTAAAT GTGCGTTAAA CAATTTGAAA 
GATTTCATTT TGACTCCTGA TTTTCCGGAA AGAAGACGTC TGATGAAAAA ATTGGCAATT 
CTTGTGTCAA AAGATCTCAA AGGTGAGAAA TCATTCTTTG AAGGTCAAAT TGTTTTGCAA 
TTTCTATCGA TAGTAGAACA CACTTTGGTT CCACAGTCAT GGAACGCATC ACGTGCTTCT 
AATTGTTGGT TATTGAAAGA GCATTTACAA ATGGCTGCAT TAAAGTATCA TTCAGGTAAT 
ATTAATGTTA TACTTGAAAA CTTGGCTGCC ACAATGGGAA GTTTCGATCT TATGTTTACA 
ACTCGAAAAA GTAAGGAACA AAAGAACAAA CTCAAATATG CAGATTTGAG TGAGCGCCAG 
GTTTTTTTTT TAGACTTGAG CTTTGATTTT ATTGCTAATA TCATAGACGT CGTCATCAAA 
CCCTCCTGGC AAAAAAACAT GGAAGACTTT CGATATCTAG CCATTATTCG TTTGCTTATG 
TGCTGGATTA AGTCATATAG ATCTATTTTG CAGTACACTC ACAGACACAG GAAGTTTTGC 
ACTTCATTCG CCTTGTTGCT GAACGACTTG ATAAATAGTC CACTGAATTG TTCAGGAAAT 
ATATATAGCC ACAGGCCGAA AAGAAGCTAT CTTTTTAGAG AAGATATTAT TTTCAGGGAA 
TTTTCTTGCA TTAACTTTGC ACTAACAGAT TTTAATGACG ATTATGTGTA TGATTCTCCC 
GACATGATTA ATAATATAAT TGGATGCCCT ACATTGACTA AAGTGCTTTC TCCAAAAGAA 
GAATGTGTTC TGCGAATTAG ATCAATAATA TTTTCTGGCA TGAAATTTTT AGAGAAAAAT 
GACACCGGCG TCATATGGAA TGCCAGCAAA TATAAGTTTG ATTTAATAAG CCCAAATATT 
AAAATAAAAC GCCAAATAGC ATTATCGGAA ATTTCCTCCA AAATAAATGT AAAAACACAA 
CAGGAAAGAG TAGTCTCTTC GAGAAAAGTT GAGGCCAAAA GAGATGAACA ACAGCGCAAA 
AGAGCCGGGA AAATAGCTGT GACAGAACTG GAAAAACAAT TTGCAAATGT CCGGAGAACA 
AAAAAATTGT CTCCGCTCCC AGAAAAAGAT GGCGTTTCTT CTGAGTTGGT AAAACATGCT 
GCTTCACGAG GGAGAAAAAC TATCACTGGC CCACTATCCT CTGATTTTCT CTCATATCCA 
GACGAAGCAA TTGATGCTGA TGAGGACATC ACCGTCCAAG TGCCAGATAC TCCTACTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XII.
Nature387(6632 Suppl)87-90(1997 May)
Johnston M,Hillier L,Riles L,Albermann K,Andr? B,Ansorge W,Benes V,Br?ckner M,Delius H,Dubois E,D?sterh?ft A,Entian KD,Floeth M,Goffeau A,Hebling U,Heumann K,Heuss-Neitzel D,Hilbert H,Hilger F,Kleine K,K?tter P,Louis EJ,Messenguy F,Mewes HW,Hoheisel JD

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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EST1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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