TUB4 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	TUB4
Entrez Gene ID	850909
Full Name	gamma-tubulin
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001182099.1	NP_013313.1	gamma-tubulin

Pathways from BioSystems

Homologies

Saccharomyces cerevisiae (baker's yeast)	TUB4	NP_013313.1
Eremothecium gossypii	AGOS_ADR076C	NP_984172.1
Kluyveromyces lactis	KLLA0C16247g	XP_452926.1

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XII.
Johnston M, Hillier L, Riles L, Albermann K, Andr? B, Ansorge W, Benes V, Br?ckner M, Delius H, Dubois E, D?sterh?ft A, Entian KD, Floeth M, Goffeau A, Hebling U, Heumann K, Heuss-Neitzel D, Hilbert H, Hilger F, Kleine K, K?tter P, Louis EJ, Messenguy F, Mewes HW, Hoheisel JD
Nature387(6632 Suppl)87-90(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

findings revealed that Rok1 protein synthesis is repressed by Psk2 and Skp1; this repression appeared to be mediated through the ROK1 uORF1; Tub4 plays a positive role in regulating Rok1 protein synthesis and likely after the uORF1-mediated inhibitory regulation
Title: Identification of Psk2, Skp1, and Tub4 as trans-acting factors for uORF-containing ROK1 mRNA in Saccharomyces cerevisiae.

The structure of the Saccharomyces cerevisiae gamma-tubulin small complex at 25-A resolution by electron microscopy, is determined.
Title: The structure of the gamma-tubulin small complex: implications of its architecture and flexibility for microtubule nucleation.

We propose that SPBs, via the tail of Tub4, promote the assembly of functional +TIP complexes before their deployment to microtubule +ends.
Title: Gamma-tubulin is required for proper recruitment and assembly of Kar9-Bim1 complexes in budding yeast.

The following TUB4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TUB4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi00548	NM_001182099.1 Latest version!	Saccharomyces cerevisiae S288C gamma-tubulin (TUB4), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TUB4 ( NM_001182099.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_013313.1 Saccharomyces cerevisiae S288C gamma-tubulin (TUB4), partial mRNA.

Price & Availability↑

CloneID	OSi00548
Clone ID Related Accession (Same CDS sequence)	NM_001182099.1
Accession Version	NM_001182099.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1422bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	gamma-tubulin
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001144). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGGGTGGAG AAATTATTAC TTTGCAAGCA GGTCAATGCG GGAACCACGT TGGTAAGTTT 
CTCTGGTCTC AATTGGCGAA AGAACACGCT ATAGGCACCG ATGGGCTATC TCAGCTTCCT 
GATTCCAGTA CGGAAAGAGA TGATGACACA AAGCCTTTCT TCCGCGAAAA CAGTAGGAAT 
AAGTTTACGC CAAGGGCTAT TATGATGGAT TCCGAGCCTA GTGTCATCGC CGACGTGGAG 
AACACATTTC GTGGGTTTTT CGACCCAAGA AATACCTGGG TAGCTTCTGA TGGCGCTAGC 
GCTGGTAATT CTTGGGCCAA TGGGTATGAT ATAGGAACTC GTAACCAGGA TGATATTCTT 
AACAAGATCG ACAAGGAGAT TGATTCTACC GACAATTTCG AAGGTTTCCA GTTGCTGCAC 
TCAGTAGCCG GAGGGACCGG TTCAGGGCTC GGATCCAACC TCTTAGAAGC GCTGTGTGAT 
CGATATCCTA AAAAAATACT CACGACATAT TCTGTGTTCC CTGCAAGATC TTCTGAGGTT 
GTTGTTCAAT CTTATAACAC TATTCTGGCT TTGAGAAGAC TGATAGAAGA TAGCGATGCA 
ACGGTGGTGT TTGATAACGC TTCGTTGCTG AATATTTCCG GTAAAGTGTT TAGAAATCCG 
AACATCGATT TACAACACAC GAATCAATTG ATATCGACCA TAATATCTTC AGTAACGAAC 
AGTATACGGT TTCCGAGTTA CATGTATTCA TCCATGTCCA GTATTTACTC TACCTTGATT 
CCTTCTCCTG AACTTCATTT CCTAAGCCCT AGTTTCACTC CATTTACATC AGATTATATT 
CACGATGATA TAGCGCATAA AGGCCATTCC AGTTATGACG TCATGTTAGA TTTATTAGAC 
CCTTCCAATT CTCTTGTCTC TACCGCGATG AACAATCCAA CATATTTTAA CGTTTATAAT 
ACAATCATTG GTAATGTAGA ACCTCGTCAG ATATCGCGTG CTATGACCAA ATTACAACAA 
CGCATCAAAT TCCCCTCATG GTCCTCTTCT GCGATGCACG TTAACATTGG AAGAAGATCG 
CCATACTTGC CTTTACAACC AAATGAAAAC GAAGTTAGCG GCATGATGTT AAGCAATATG 
TCTACCGTGG TGAACGTCTT TGAGAATGCG TGCAATACTT TTGACAAAGT ATTTGCCAAG 
GGCGCATTTT TAAATAATTA TAATGTAGGC GACTTGTTCC AATCAATGCA GAACGTTCAA 
GATGAATTCG CCGAGTCAAG AGAAGTAGTA CAAAGCCTGA TGGAGGATTA TGTAGCTGCA 
GAACAAGATT CGTACTTGGA CGATGTACTT GTTGATGATG AAAATATGGT TGGCGAGTTG 
GAAGAGGACC TGGATGCCGA CGGTGATCAT AAATTAGTAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_013313.1
CDS	1..1422
Translation	MGGEIITLQAGQCGNHVGKFLWSQLAKEHAIGTDGLSQLPDSSTERDDDTKPFFRENSRNKFTPRAIMMDSEPSVIADVENTFRGFFDPRNTWVASDGASAGNSWANGYDIGTRNQDDILNKIDKEIDSTDNFEGFQLLHSVAGGTGSGLGSNLLEALCDRYPKKILTTYSVFPARSSEVVVQSYNTILALRRLIEDSDATVVFDNASLLNISGKVFRNPNIDLQHTNQLISTIISSVTNSIRFPSYMYSSMSSIYSTLIPSPELHFLSPSFTPFTSDYIHDDIAHKGHSSYDVMLDLLDPSNSLVSTAMNNPTYFNVYNTIIGNVEPRQISRAMTKLQQRIKFPSWSSSAMHVNIGRRSPYLPLQPNENEVSGMMLSNMSTVVNVFENACNTFDKVFAKGAFLNNYNVGDLFQSMQNVQDEFAESREVVQSLMEDYVAAEQDSYLDDVLVDDENMVGELEEDLDADGDHKLV

Target ORF information:

RefSeq Version	NM_001182099.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C gamma-tubulin (TUB4), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001182099.1

ORF Insert Sequence:

ATGGGTGGAG AAATTATTAC TTTGCAAGCA GGTCAATGCG GGAACCACGT TGGTAAGTTT 
CTCTGGTCTC AATTGGCGAA AGAACACGCT ATAGGCACCG ATGGGCTATC TCAGCTTCCT 
GATTCCAGTA CGGAAAGAGA TGATGACACA AAGCCTTTCT TCCGCGAAAA CAGTAGGAAT 
AAGTTTACGC CAAGGGCTAT TATGATGGAT TCCGAGCCTA GTGTCATCGC CGACGTGGAG 
AACACATTTC GTGGGTTTTT CGACCCAAGA AATACCTGGG TAGCTTCTGA TGGCGCTAGC 
GCTGGTAATT CTTGGGCCAA TGGGTATGAT ATAGGAACTC GTAACCAGGA TGATATTCTT 
AACAAGATCG ACAAGGAGAT TGATTCTACC GACAATTTCG AAGGTTTCCA GTTGCTGCAC 
TCAGTAGCCG GAGGGACCGG TTCAGGGCTC GGATCCAACC TCTTAGAAGC GCTGTGTGAT 
CGATATCCTA AAAAAATACT CACGACATAT TCTGTGTTCC CTGCAAGATC TTCTGAGGTT 
GTTGTTCAAT CTTATAACAC TATTCTGGCT TTGAGAAGAC TGATAGAAGA TAGCGATGCA 
ACGGTGGTGT TTGATAACGC TTCGTTGCTG AATATTTCCG GTAAAGTGTT TAGAAATCCG 
AACATCGATT TACAACACAC GAATCAATTG ATATCGACCA TAATATCTTC AGTAACGAAC 
AGTATACGGT TTCCGAGTTA CATGTATTCA TCCATGTCCA GTATTTACTC TACCTTGATT 
CCTTCTCCTG AACTTCATTT CCTAAGCCCT AGTTTCACTC CATTTACATC AGATTATATT 
CACGATGATA TAGCGCATAA AGGCCATTCC AGTTATGACG TCATGTTAGA TTTATTAGAC 
CCTTCCAATT CTCTTGTCTC TACCGCGATG AACAATCCAA CATATTTTAA CGTTTATAAT 
ACAATCATTG GTAATGTAGA ACCTCGTCAG ATATCGCGTG CTATGACCAA ATTACAACAA 
CGCATCAAAT TCCCCTCATG GTCCTCTTCT GCGATGCACG TTAACATTGG AAGAAGATCG 
CCATACTTGC CTTTACAACC AAATGAAAAC GAAGTTAGCG GCATGATGTT AAGCAATATG 
TCTACCGTGG TGAACGTCTT TGAGAATGCG TGCAATACTT TTGACAAAGT ATTTGCCAAG 
GGCGCATTTT TAAATAATTA TAATGTAGGC GACTTGTTCC AATCAATGCA GAACGTTCAA 
GATGAATTCG CCGAGTCAAG AGAAGTAGTA CAAAGCCTGA TGGAGGATTA TGTAGCTGCA 
GAACAAGATT CGTACTTGGA CGATGTACTT GTTGATGATG AAAATATGGT TGGCGAGTTG 
GAAGAGGACC TGGATGCCGA CGGTGATCAT AAATTAGTAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XII.
Nature387(6632 Suppl)87-90(1997 May)
Johnston M,Hillier L,Riles L,Albermann K,Andr? B,Ansorge W,Benes V,Br?ckner M,Delius H,Dubois E,D?sterh?ft A,Entian KD,Floeth M,Goffeau A,Hebling U,Heumann K,Heuss-Neitzel D,Hilbert H,Hilger F,Kleine K,K?tter P,Louis EJ,Messenguy F,Mewes HW,Hoheisel JD

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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TUB4 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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