MDL1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	MDL1
Entrez Gene ID	850885
Full Name	ATP-binding cassette permease MDL1
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001182075.1	NP_013289.1	ATP-binding cassette permease MDL1

Pathways from BioSystems

Homologies

Eremothecium gossypii	AGOS_AFR683C	NP_986231.1
Saccharomyces cerevisiae (baker's yeast)	MDL1	NP_013289.1
Kluyveromyces lactis	KLLA0C01991g	XP_452285.1

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XII.
Johnston M, Hillier L, Riles L, Albermann K, Andr? B, Ansorge W, Benes V, Br?ckner M, Delius H, Dubois E, D?sterh?ft A, Entian KD, Floeth M, Goffeau A, Hebling U, Heumann K, Heuss-Neitzel D, Hilbert H, Hilger F, Kleine K, K?tter P, Louis EJ, Messenguy F, Mewes HW, Hoheisel JD
Nature387(6632 Suppl)87-90(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Tom70 contributes to the presequence import pathway by recognition of the targeting signal of the Mdl1 precursor.
Title: A presequence-binding groove in Tom70 supports import of Mdl1 into mitochondria.

Modes of insertion into the mitochondrial membrane of individual segments of Mdl1 and Mdl2 differ and is dependent on domain sequence context.
Title: Mode of membrane insertion of individual transmembrane segments in Mdl1 and Mdl2, multi-spanning mitochondrial ABC transporters.

A first three-dimensional map of the mitochondrial ATP-binding cassette transporter Mdl1 complex reveals a homodimeric complex in an open conformation.
Title: Structural and functional fingerprint of the mitochondrial ATP-binding cassette transporter Mdl1 from Saccharomyces cerevisiae.

These data explain contradicting observations on the rate-limiting step of various ABC proteins such as Mdl1 and demonstrate that dimer formation is an important step in the ATP hydrolysis cycle.
Title: Kinetics of the ATP hydrolysis cycle of the nucleotide-binding domain of Mdl1 studied by a novel site-specific labeling technique.

The following MDL1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MDL1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi00526	NM_001182075.1 Latest version!	Saccharomyces cerevisiae S288C ATP-binding cassette permease MDL1 (MDL1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MDL1 ( NM_001182075.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_013289.1 Saccharomyces cerevisiae S288C ATP-binding cassette permease MDL1 (MDL1), partial mRNA.

Price & Availability↑

CloneID	OSi00526
Clone ID Related Accession (Same CDS sequence)	NM_001182075.1
Accession Version	NM_001182075.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2088bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	ATP-binding cassette permease MDL1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001144). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGATTGTAA GAATGATACG TCTTTGTAAA GGTCCAAAGT TGTTGCGAAG TCAATTCGCA 
TCAGCAAGTG CACTATATTC AACCAAGTCA TTGTTCAAAC CTCCCATGTA CCAAAAAGCG 
GAAATTAATT TGATTATTCC ACATAGAAAG CACTTTTTGT TGCGGTCGAT TCGATTACAA 
TCAGACATTG CGCAAGGAAA GAAGTCCACC AAGCCCACTT TAAAGTTGTC CAATGCAAAT 
TCAAAATCCT CGGGGTTTAA AGACATTAAA CGGCTATTCG TCTTATCTAA ACCGGAATCC 
AAGTACATTG GTCTTGCCCT CCTTTTAATC CTCATTTCAA GTTCAGTTAG TATGGCTGTA 
CCTTCCGTTA TCGGTAAATT ATTAGACTTG GCTTCCGAAA GTGACGGCGA AGATGAAGAG 
GGCTCAAAAA GCAATAAGTT ATATGGTTTT ACGAAGAAGC AATTTTTCAC AGCATTAGGA 
GCAGTATTTA TAATTGGAGC AGTTGCTAAT GCAAGCAGAA TCATCATTTT AAAGGTCACC 
GGTGAGAGAC TGGTCGCAAG ATTAAGAACG AGAACAATGA AAGCTGCATT AGATCAAGAT 
GCCACATTTT TAGATACTAA TCGTGTCGGT GATTTGATCT CAAGATTATC ATCTGATGCA 
TCTATAGTGG CCAAATCGGT CACACAAAAC GTCTCTGATG GAACAAGGGC AATTATTCAA 
GGGTTTGTCG GTTTTGGCAT GATGAGCTTC CTCTCGTGGA AATTGACTTG CGTAATGATG 
ATTTTAGCCC CTCCTTTAGG TGCTATGGCA CTGATATATG GTAGGAAAAT ACGAAACCTA 
TCAAGACAGT TACAAACCTC AGTAGGTGGG TTAACAAAAG TGGCAGAAGA GCAATTAAAT 
GCTACTAGGA CAATTCAAGC ATATGGTGGT GAAAAAAACG AGGTTCGCCG CTATGCAAAA 
GAGGTTAGAA ATGTATTTCA TATTGGTCTG AAGGAAGCGG TTACTTCCGG TTTATTCTTC 
GGAAGTACTG GGCTAGTTGG CAACACTGCA ATGCTGTCGT TATTATTGGT TGGAACAAGC 
ATGATTCAAA GTGGTTCAAT GACTGTCGGT GAATTATCTA GTTTCATGAT GTATGCCGTG 
TATACCGGAA GTTCATTATT TGGTTTATCG AGCTTCTATT CAGAACTTAT GAAAGGTGCT 
GGTGCGGCTG CCAGGGTTTT TGAATTAAAT GACCGTAAGC CATTGATTCG TCCGACTATT 
GGAAAGGATC CTGTGTCATT AGCCCAAAAA CCCATCGTTT TCAAAAACGT GTCATTCACT 
TATCCCACTC GGCCCAAACA CCAGATTTTC AAGGATTTGA ATATTACTAT CAAGCCTGGT 
GAACACGTTT GCGCTGTCGG TCCATCAGGA AGCGGCAAAT CAACAATTGC GTCTTTGTTG 
CTCAGGTACT ACGATGTGAA CTCAGGATCG ATCGAATTTG GTGATGAAGA CATCAGAAAT 
TTCAACTTGA GGAAGTATCG AAGACTAATA GGATACGTGC AACAAGAACC ACTACTTTTC 
AATGGGACTA TTCTGGACAA TATCCTCTAC TGCATTCCGC CTGAAATTGC GGAGCAGGAT 
GATCGTATTA GACGTGCTAT TGGAAAAGCT AATTGCACAA AATTTTTGGC CAATTTTCCA 
GATGGATTGC AGACTATGGT TGGTGCTAGA GGCGCGCAAT TGTCCGGTGG TCAAAAGCAA 
AGAATTGCAT TAGCTAGAGC GTTTTTACTA GATCCTGCCG TCCTTATTTT AGATGAAGCA 
ACCAGTGCCC TTGACTCTCA AAGTGAAGAA ATAGTTGCCA AAAATCTTCA GAGACGTGTG 
GAAAGGGGGT TCACTACTAT ATCAATTGCA CATAGGCTTT CGACGATCAA ACACAGTACT 
AGGGTGATTG TGTTAGGAAA ACATGGCTCA GTGGTTGAAA CCGGTTCATT CCGAGATCTA 
ATCGCCATTC CTAATAGTGA ACTAAATGCG CTGCTTGCCG AACAGCAGGA CGAGGAAGGA 
AAAGGGGGAG TGATAGATTT GGACAATAGT GTTGCCCGGG AAGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_013289.1
CDS	1..2088
Translation	MIVRMIRLCKGPKLLRSQFASASALYSTKSLFKPPMYQKAEINLIIPHRKHFLLRSIRLQSDIAQGKKSTKPTLKLSNANSKSSGFKDIKRLFVLSKPESKYIGLALLLILISSSVSMAVPSVIGKLLDLASESDGEDEEGSKSNKLYGFTKKQFFTALGAVFIIGAVANASRIIILKVTGERLVARLRTRTMKAALDQDATFLDTNRVGDLISRLSSDASIVAKSVTQNVSDGTRAIIQGFVGFGMMSFLSWKLTCVMMILAPPLGAMALIYGRKIRNLSRQLQTSVGGLTKVAEEQLNATRTIQAYGGEKNEVRRYAKEVRNVFHIGLKEAVTSGLFFGSTGLVGNTAMLSLLLVGTSMIQSGSMTVGELSSFMMYAVYTGSSLFGLSSFYSELMKGAGAAARVFELNDRKPLIRPTIGKDPVSLAQKPIVFKNVSFTYPTRPKHQIFKDLNITIKPGEHVCAVGPSGSGKSTIASLLLRYYDVNSGSIEFGDEDIRNFNLRKYRRLIGYVQQEPLLFNGTILDNILYCIPPEIAEQDDRIRRAIGKANCTKFLANFPDGLQTMVGARGAQLSGGQKQRIALARAFLLDPAVLILDEATSALDSQSEEIVAKNLQRRVERGFTTISIAHRLSTIKHSTRVIVLGKHGSVVETGSFRDLIAIPNSELNALLAEQQDEEGKGGVIDLDNSVAREV

Target ORF information:

RefSeq Version	NM_001182075.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C ATP-binding cassette permease MDL1 (MDL1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001182075.1

ORF Insert Sequence:

ATGATTGTAA GAATGATACG TCTTTGTAAA GGTCCAAAGT TGTTGCGAAG TCAATTCGCA 
TCAGCAAGTG CACTATATTC AACCAAGTCA TTGTTCAAAC CTCCCATGTA CCAAAAAGCG 
GAAATTAATT TGATTATTCC ACATAGAAAG CACTTTTTGT TGCGGTCGAT TCGATTACAA 
TCAGACATTG CGCAAGGAAA GAAGTCCACC AAGCCCACTT TAAAGTTGTC CAATGCAAAT 
TCAAAATCCT CGGGGTTTAA AGACATTAAA CGGCTATTCG TCTTATCTAA ACCGGAATCC 
AAGTACATTG GTCTTGCCCT CCTTTTAATC CTCATTTCAA GTTCAGTTAG TATGGCTGTA 
CCTTCCGTTA TCGGTAAATT ATTAGACTTG GCTTCCGAAA GTGACGGCGA AGATGAAGAG 
GGCTCAAAAA GCAATAAGTT ATATGGTTTT ACGAAGAAGC AATTTTTCAC AGCATTAGGA 
GCAGTATTTA TAATTGGAGC AGTTGCTAAT GCAAGCAGAA TCATCATTTT AAAGGTCACC 
GGTGAGAGAC TGGTCGCAAG ATTAAGAACG AGAACAATGA AAGCTGCATT AGATCAAGAT 
GCCACATTTT TAGATACTAA TCGTGTCGGT GATTTGATCT CAAGATTATC ATCTGATGCA 
TCTATAGTGG CCAAATCGGT CACACAAAAC GTCTCTGATG GAACAAGGGC AATTATTCAA 
GGGTTTGTCG GTTTTGGCAT GATGAGCTTC CTCTCGTGGA AATTGACTTG CGTAATGATG 
ATTTTAGCCC CTCCTTTAGG TGCTATGGCA CTGATATATG GTAGGAAAAT ACGAAACCTA 
TCAAGACAGT TACAAACCTC AGTAGGTGGG TTAACAAAAG TGGCAGAAGA GCAATTAAAT 
GCTACTAGGA CAATTCAAGC ATATGGTGGT GAAAAAAACG AGGTTCGCCG CTATGCAAAA 
GAGGTTAGAA ATGTATTTCA TATTGGTCTG AAGGAAGCGG TTACTTCCGG TTTATTCTTC 
GGAAGTACTG GGCTAGTTGG CAACACTGCA ATGCTGTCGT TATTATTGGT TGGAACAAGC 
ATGATTCAAA GTGGTTCAAT GACTGTCGGT GAATTATCTA GTTTCATGAT GTATGCCGTG 
TATACCGGAA GTTCATTATT TGGTTTATCG AGCTTCTATT CAGAACTTAT GAAAGGTGCT 
GGTGCGGCTG CCAGGGTTTT TGAATTAAAT GACCGTAAGC CATTGATTCG TCCGACTATT 
GGAAAGGATC CTGTGTCATT AGCCCAAAAA CCCATCGTTT TCAAAAACGT GTCATTCACT 
TATCCCACTC GGCCCAAACA CCAGATTTTC AAGGATTTGA ATATTACTAT CAAGCCTGGT 
GAACACGTTT GCGCTGTCGG TCCATCAGGA AGCGGCAAAT CAACAATTGC GTCTTTGTTG 
CTCAGGTACT ACGATGTGAA CTCAGGATCG ATCGAATTTG GTGATGAAGA CATCAGAAAT 
TTCAACTTGA GGAAGTATCG AAGACTAATA GGATACGTGC AACAAGAACC ACTACTTTTC 
AATGGGACTA TTCTGGACAA TATCCTCTAC TGCATTCCGC CTGAAATTGC GGAGCAGGAT 
GATCGTATTA GACGTGCTAT TGGAAAAGCT AATTGCACAA AATTTTTGGC CAATTTTCCA 
GATGGATTGC AGACTATGGT TGGTGCTAGA GGCGCGCAAT TGTCCGGTGG TCAAAAGCAA 
AGAATTGCAT TAGCTAGAGC GTTTTTACTA GATCCTGCCG TCCTTATTTT AGATGAAGCA 
ACCAGTGCCC TTGACTCTCA AAGTGAAGAA ATAGTTGCCA AAAATCTTCA GAGACGTGTG 
GAAAGGGGGT TCACTACTAT ATCAATTGCA CATAGGCTTT CGACGATCAA ACACAGTACT 
AGGGTGATTG TGTTAGGAAA ACATGGCTCA GTGGTTGAAA CCGGTTCATT CCGAGATCTA 
ATCGCCATTC CTAATAGTGA ACTAAATGCG CTGCTTGCCG AACAGCAGGA CGAGGAAGGA 
AAAGGGGGAG TGATAGATTT GGACAATAGT GTTGCCCGGG AAGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XII.
Nature387(6632 Suppl)87-90(1997 May)
Johnston M,Hillier L,Riles L,Albermann K,Andr? B,Ansorge W,Benes V,Br?ckner M,Delius H,Dubois E,D?sterh?ft A,Entian KD,Floeth M,Goffeau A,Hebling U,Heumann K,Heuss-Neitzel D,Hilbert H,Hilger F,Kleine K,K?tter P,Louis EJ,Messenguy F,Mewes HW,Hoheisel JD

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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MDL1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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