SWI6 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	SWI6
Entrez Gene ID	850879
Full Name	transcriptional regulator SWI6
Synonyms	PSL8,SDS11
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001182069.1	NP_013283.1	transcriptional regulator SWI6

Pathways from BioSystems

Homologies

Saccharomyces cerevisiae (baker's yeast)	SWI6	NP_013283.1
Eremothecium gossypii	AGOS_AFR690C	NP_986238.2
Kluyveromyces lactis	KLLA0C01826g	XP_452277.1

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XII.
Johnston M, Hillier L, Riles L, Albermann K, Andr? B, Ansorge W, Benes V, Br?ckner M, Delius H, Dubois E, D?sterh?ft A, Entian KD, Floeth M, Goffeau A, Hebling U, Heumann K, Heuss-Neitzel D, Hilbert H, Hilger F, Kleine K, K?tter P, Louis EJ, Messenguy F, Mewes HW, Hoheisel JD
Nature387(6632 Suppl)87-90(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Swi4/Swi6 (SBF) factor binding at HO follows a temporal cascade, with SBF bound at upstream regulatory sequence (URS2-L) serving to relay a signal from URS1 to the SBF sites in URS2-R that ultimately activate gene expression.
Title: Spatiotemporal cascade of transcription factor binding required for promoter activation.

study identifies a region of the carboxy terminus of Swi6 that is required for Nrm1 and Whi5 binding to their target transcription factors and show that mutation of this region overrides the repression of MBF- and SBF-regulated genes by Nrm1 and Whi5.
Title: Repression of G1/S transcription is mediated via interaction of the GTB motifs of Nrm1 and Whi5 with Swi6.

We found that the transcriptional cofactor Swi6p and activator Swi4p, as well as the G(2)/M-specific cyclin Clb2p, are required in a microtubule-destabilizing environment
Title: Cell cycle regulators interact with pathways that modulate microtubule stability in Saccharomyces cerevisiae.

oxidative stress signaling for cell cycle regulation occurs through oxidation of the G(1)/S-specific transcription factor Swi6p and consequently leads to suppression of the expression of G(1) cyclins and a delay in cells entering the cell cycle.
Title: Cell cycle sensing of oxidative stress in Saccharomyces cerevisiae by oxidation of a specific cysteine residue in the transcription factor Swi6p.

Data show that in Swi6 the structural and physical features of the nucleic acid binding interface have been completely eradicated.
Title: Structure of the amino-terminal domain from the cell-cycle regulator Swi6.

The following SWI6 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SWI6 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi00521	NM_001182069.1 Latest version!	Saccharomyces cerevisiae S288C transcriptional regulator SWI6 (SWI6), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SWI6 ( NM_001182069.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_013283.1 Saccharomyces cerevisiae S288C transcriptional regulator SWI6 (SWI6), partial mRNA.

Price & Availability↑

CloneID	OSi00521
Clone ID Related Accession (Same CDS sequence)	NM_001182069.1
Accession Version	NM_001182069.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2412bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	transcriptional regulator SWI6
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001144). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGGCGTTGG AAGAAGTGGT ACGATACTTA GGACCTCACA ATGAGATCCC TTTGACACTC 
ACTAGAGACT CGGAGACTGG CCATTTCCTC CTGAAACATT TTCTGCCCAT TTTGCAGCAA 
TATCATGACA CGGGGAATAT TAACGAGACC AACCCCGATA GTTTCCCCAC TGATGAGGAA 
AGAAATAAAT TACTGGCACA TTATGGAATT GCTGTAAATA CAGACGACCG AGGTGAGTTA 
TGGATAGAGC TGGAAAAATG TTTACAATTA TTAAACATGC TAAATTTATT CGGTTTGTTC 
CAGGATGCAT TCGAATTTGA GGAGCCTGAA ACAGATCAGG ATGAAGAAGA TCCCAGCCAT 
TCGAAACTAC CAGAAAACAA GACCAAAAGT GAAAATTCTA AGGATAATAT CAGCTCAAAG 
AGGATTAATA ATTTACAAGA TATGAGCCTG GATTCTGATG CACACAGAGA ATTAGGCTCT 
CCTTTAAAAA AACTAAAAAT AGATACCTCT GTAATAGATG CTGAGAGTGA CTCCACTCCG 
AATACTGCCA GAGGCAAGCC TAACGATGAT ATTAATAAGG GCCCTAGCGG CGACAATGAA 
AATAATGGCA CTGATGACAA TGACAGAACC GCTGGACCTA TCATAACATT CACTCATGAC 
CTAACTTCTG ACTTTTTAAG CAGTCCACTG AAAATCATGA AAGCACTACC TTCTCCAGTT 
GTAAATGATA ATGAACAGAA GATGAAACTA GAGGCATTCT TACAACGGTT GTTATTTCCA 
GAAATTCAAG AAATGCCTAC ATCCCTTAAT AATGACAGCA GTAATAGAAA TTCAGAAGGG 
GGGAGCTCAA ACCAACAACA ACAGCACGTA TCATTTGATA GCCTTTTGCA AGAGGTAAAC 
GACGCTTTTC CTAATACTCA ATTAAATCTT AATATTCCTG TAGATGAGCA TGGAAACACA 
CCATTACATT GGCTGACTTC AATAGCAAAC CTGGAATTAG TGAAACACCT GGTTAAGCAT 
GGTTCAAACA GATTATATGG TGATAATATG GGGGAGTCAT GCCTAGTGAA AGCTGTCAAA 
TCAGTAAATA ATTACGACTC TGGTACTTTT GAGGCACTTC TAGATTATTT ATATCCATGT 
TTAATTTTGG AAGACTCAAT GAATAGAACA ATTTTGCACC ATATCATTAT TACGTCTGGT 
ATGACTGGCT GTTCAGCAGC TGCGAAATAT TATTTAGATA TTTTAATGGG ATGGATTGTC 
AAGAAACAAA ATAGACCCAT TCAAAGTGGT ACTAACGAAA AAGAAAGCAA ACCGAATGAC 
AAAAATGGGG AAAGAAAGGA CTCTATACTA GAAAATTTGG ACCTGAAATG GATAATAGCA 
AACATGCTCA ATGCGCAGGA CTCTAATGGC GATACTTGCT TGAACATTGC AGCAAGATTG 
GGAAACATTT CAATAGTAGA CGCTTTATTA GATTACGGTG CTGACCCATT TATTGCAAAC 
AAATCAGGGT TGAGACCCGT GGATTTTGGG GCAGGTACTT CAAAATTACA AAATACGAAC 
GGCGGTGACG AAAACTCGAA GATGGTCTCT AAGGGTGATT ACGACGGTCA AAAAAATGGA 
AAGGCCAAAA AAATAAGGTC TCAACTGTTG AAAAACCCAC CTGAAACAAC TTCGTTAATT 
AATGATGTCC AAAATTTACT GAATTCAATC TCGAAAGATT ATGAGAATGA AACAGTGCAA 
TATAATGAGA AATTAGAAAA ACTACACAAG GAATTGAACG AACAGCGAGA AGAATTAGCT 
AATTCTAGAG AACAACTAGC AAATGTGAAG CAATTGAAAG ATGAATACTC ACTAATGCAA 
GAGCAATTGA CCAATTTAAA AGCAGGAATT GAAGAAGAAG AGGAAAGTTT TAGGGAAGAG 
AGCAAAAAGC TAGGAATAAT TGCAGATGAA AGTTCAGGTA TTGATTGGGA CTCTAGCGAA 
TATGATGCAG ATGAACCCTT CAAAGTAGAG TTCCTTTCAG ATTTCTTGGA GGATAAATTA 
CAAAAGAATT ATGAAGGCGA TATTTCCAAA CTATTAGAAG CGGAGTCGAA AGAGCAGATA 
ATGGAACAGA TACGAAATCA ACTTCCCGCA GAAAAAATCC AATCGATGCT TCCACCAACG 
GTCTTATTGA AGGCAAGAAT AAATGCATAC AAGCGGAATG ATAAACACCT AACCAATGTA 
TTGGATACAA TCAGCACCAA ACAATCAGAA TTAGAGAATA AATTTAGAAG AGTGTTATCT 
TTATGTTTGA AAATCGACGA AAATAAAGTT GACAATATGC TTGATGGTTT GTTACAGGCC 
ATATCTTCTG AAGACCCACA AGACATTGAC ACTGACGAAA TGCAAGATTT TTTAAAAAAG 
CATGCTTCAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_013283.1
CDS	1..2412
Translation	MALEEVVRYLGPHNEIPLTLTRDSETGHFLLKHFLPILQQYHDTGNINETNPDSFPTDEERNKLLAHYGIAVNTDDRGELWIELEKCLQLLNMLNLFGLFQDAFEFEEPETDQDEEDPSHSKLPENKTKSENSKDNISSKRINNLQDMSLDSDAHRELGSPLKKLKIDTSVIDAESDSTPNTARGKPNDDINKGPSGDNENNGTDDNDRTAGPIITFTHDLTSDFLSSPLKIMKALPSPVVNDNEQKMKLEAFLQRLLFPEIQEMPTSLNNDSSNRNSEGGSSNQQQQHVSFDSLLQEVNDAFPNTQLNLNIPVDEHGNTPLHWLTSIANLELVKHLVKHGSNRLYGDNMGESCLVKAVKSVNNYDSGTFEALLDYLYPCLILEDSMNRTILHHIIITSGMTGCSAAAKYYLDILMGWIVKKQNRPIQSGTNEKESKPNDKNGERKDSILENLDLKWIIANMLNAQDSNGDTCLNIAARLGNISIVDALLDYGADPFIANKSGLRPVDFGAGTSKLQNTNGGDENSKMVSKGDYDGQKNGKAKKIRSQLLKNPPETTSLINDVQNLLNSISKDYENETVQYNEKLEKLHKELNEQREELANSREQLANVKQLKDEYSLMQEQLTNLKAGIEEEEESFREESKKLGIIADESSGIDWDSSEYDADEPFKVEFLSDFLEDKLQKNYEGDISKLLEAESKEQIMEQIRNQLPAEKIQSMLPPTVLLKARINAYKRNDKHLTNVLDTISTKQSELENKFRRVLSLCLKIDENKVDNMLDGLLQAISSEDPQDIDTDEMQDFLKKHAS

Target ORF information:

RefSeq Version	NM_001182069.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C transcriptional regulator SWI6 (SWI6), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001182069.1

ORF Insert Sequence:

ATGGCGTTGG AAGAAGTGGT ACGATACTTA GGACCTCACA ATGAGATCCC TTTGACACTC 
ACTAGAGACT CGGAGACTGG CCATTTCCTC CTGAAACATT TTCTGCCCAT TTTGCAGCAA 
TATCATGACA CGGGGAATAT TAACGAGACC AACCCCGATA GTTTCCCCAC TGATGAGGAA 
AGAAATAAAT TACTGGCACA TTATGGAATT GCTGTAAATA CAGACGACCG AGGTGAGTTA 
TGGATAGAGC TGGAAAAATG TTTACAATTA TTAAACATGC TAAATTTATT CGGTTTGTTC 
CAGGATGCAT TCGAATTTGA GGAGCCTGAA ACAGATCAGG ATGAAGAAGA TCCCAGCCAT 
TCGAAACTAC CAGAAAACAA GACCAAAAGT GAAAATTCTA AGGATAATAT CAGCTCAAAG 
AGGATTAATA ATTTACAAGA TATGAGCCTG GATTCTGATG CACACAGAGA ATTAGGCTCT 
CCTTTAAAAA AACTAAAAAT AGATACCTCT GTAATAGATG CTGAGAGTGA CTCCACTCCG 
AATACTGCCA GAGGCAAGCC TAACGATGAT ATTAATAAGG GCCCTAGCGG CGACAATGAA 
AATAATGGCA CTGATGACAA TGACAGAACC GCTGGACCTA TCATAACATT CACTCATGAC 
CTAACTTCTG ACTTTTTAAG CAGTCCACTG AAAATCATGA AAGCACTACC TTCTCCAGTT 
GTAAATGATA ATGAACAGAA GATGAAACTA GAGGCATTCT TACAACGGTT GTTATTTCCA 
GAAATTCAAG AAATGCCTAC ATCCCTTAAT AATGACAGCA GTAATAGAAA TTCAGAAGGG 
GGGAGCTCAA ACCAACAACA ACAGCACGTA TCATTTGATA GCCTTTTGCA AGAGGTAAAC 
GACGCTTTTC CTAATACTCA ATTAAATCTT AATATTCCTG TAGATGAGCA TGGAAACACA 
CCATTACATT GGCTGACTTC AATAGCAAAC CTGGAATTAG TGAAACACCT GGTTAAGCAT 
GGTTCAAACA GATTATATGG TGATAATATG GGGGAGTCAT GCCTAGTGAA AGCTGTCAAA 
TCAGTAAATA ATTACGACTC TGGTACTTTT GAGGCACTTC TAGATTATTT ATATCCATGT 
TTAATTTTGG AAGACTCAAT GAATAGAACA ATTTTGCACC ATATCATTAT TACGTCTGGT 
ATGACTGGCT GTTCAGCAGC TGCGAAATAT TATTTAGATA TTTTAATGGG ATGGATTGTC 
AAGAAACAAA ATAGACCCAT TCAAAGTGGT ACTAACGAAA AAGAAAGCAA ACCGAATGAC 
AAAAATGGGG AAAGAAAGGA CTCTATACTA GAAAATTTGG ACCTGAAATG GATAATAGCA 
AACATGCTCA ATGCGCAGGA CTCTAATGGC GATACTTGCT TGAACATTGC AGCAAGATTG 
GGAAACATTT CAATAGTAGA CGCTTTATTA GATTACGGTG CTGACCCATT TATTGCAAAC 
AAATCAGGGT TGAGACCCGT GGATTTTGGG GCAGGTACTT CAAAATTACA AAATACGAAC 
GGCGGTGACG AAAACTCGAA GATGGTCTCT AAGGGTGATT ACGACGGTCA AAAAAATGGA 
AAGGCCAAAA AAATAAGGTC TCAACTGTTG AAAAACCCAC CTGAAACAAC TTCGTTAATT 
AATGATGTCC AAAATTTACT GAATTCAATC TCGAAAGATT ATGAGAATGA AACAGTGCAA 
TATAATGAGA AATTAGAAAA ACTACACAAG GAATTGAACG AACAGCGAGA AGAATTAGCT 
AATTCTAGAG AACAACTAGC AAATGTGAAG CAATTGAAAG ATGAATACTC ACTAATGCAA 
GAGCAATTGA CCAATTTAAA AGCAGGAATT GAAGAAGAAG AGGAAAGTTT TAGGGAAGAG 
AGCAAAAAGC TAGGAATAAT TGCAGATGAA AGTTCAGGTA TTGATTGGGA CTCTAGCGAA 
TATGATGCAG ATGAACCCTT CAAAGTAGAG TTCCTTTCAG ATTTCTTGGA GGATAAATTA 
CAAAAGAATT ATGAAGGCGA TATTTCCAAA CTATTAGAAG CGGAGTCGAA AGAGCAGATA 
ATGGAACAGA TACGAAATCA ACTTCCCGCA GAAAAAATCC AATCGATGCT TCCACCAACG 
GTCTTATTGA AGGCAAGAAT AAATGCATAC AAGCGGAATG ATAAACACCT AACCAATGTA 
TTGGATACAA TCAGCACCAA ACAATCAGAA TTAGAGAATA AATTTAGAAG AGTGTTATCT 
TTATGTTTGA AAATCGACGA AAATAAAGTT GACAATATGC TTGATGGTTT GTTACAGGCC 
ATATCTTCTG AAGACCCACA AGACATTGAC ACTGACGAAA TGCAAGATTT TTTAAAAAAG 
CATGCTTCAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XII.
Nature387(6632 Suppl)87-90(1997 May)
Johnston M,Hillier L,Riles L,Albermann K,Andr? B,Ansorge W,Benes V,Br?ckner M,Delius H,Dubois E,D?sterh?ft A,Entian KD,Floeth M,Goffeau A,Hebling U,Heumann K,Heuss-Neitzel D,Hilbert H,Hilger F,Kleine K,K?tter P,Louis EJ,Messenguy F,Mewes HW,Hoheisel JD

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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SWI6 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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