UBR2 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	UBR2
Entrez Gene ID	850711
Full Name	putative ubiquitin-protein ligase UBR2
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001181911.2	NP_013124.1	putative ubiquitin-protein ligase UBR2

Pathways from BioSystems

Homologies

Saccharomyces cerevisiae (baker's yeast)	UBR2	NP_013124.1
Eremothecium gossypii	AGOS_AAL147C	NP_982395.2
Kluyveromyces lactis	KLLA0F15367g	XP_455770.1

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XII.
Johnston M, Hillier L, Riles L, Albermann K, Andr? B, Ansorge W, Benes V, Br?ckner M, Delius H, Dubois E, D?sterh?ft A, Entian KD, Floeth M, Goffeau A, Hebling U, Heumann K, Heuss-Neitzel D, Hilbert H, Hilger F, Kleine K, K?tter P, Louis EJ, Messenguy F, Mewes HW, Hoheisel JD
Nature387(6632 Suppl)87-90(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Psh1 and Ubr2 synergistically control the amount of proteins at the kinetochore.
Title: Synergistic Control of Kinetochore Protein Levels by Psh1 and Ubr2.

Although Mub1/Ubr2 are not stable kinetochore components in vivo, they regulate the levels of the conserved outer kinetochore protein Dsn1 via ubiquitylation
Title: The Mub1/Ubr2 ubiquitin ligase complex regulates the conserved Dsn1 kinetochore protein.

Data show that ubiquitin-protein ligases Ubr1 and Ubr2 have opposing roles in Ste11DeltaNK444R-GFP aggregation.
Title: A network of ubiquitin ligases is important for the dynamics of misfolded protein aggregates in yeast.

Data suggest that Ubr1 and Ubr2 represent E3 components of a novel quality control pathway for proteins synthesized on cytosolic ribosomes.
Title: Ubr1 and Ubr2 function in a quality control pathway for degradation of unfolded cytosolic proteins.

Rpn4 is a physiological substrate of the Ubr2 ubiquitin ligase
Title: Rpn4 is a physiological substrate of the Ubr2 ubiquitin ligase.

The following UBR2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the UBR2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi00376	NM_001181911.2 Latest version!	Saccharomyces cerevisiae S288C putative ubiquitin-protein ligase UBR2 (UBR2), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

UBR2 ( NM_001181911.2 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_013124.1 Saccharomyces cerevisiae S288C putative ubiquitin-protein ligase UBR2 (UBR2), partial mRNA.

Price & Availability↑

CloneID	OSi00376
Clone ID Related Accession (Same CDS sequence)	NM_001181911.2
Accession Version	NM_001181911.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5619bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	putative ubiquitin-protein ligase UBR2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001144). On Apr 26, 2011 this sequence version replaced NM_001181911.1. ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGGAAGATT CTGACCTGTC TATAACCAAT ATAAGAGATT TTCTTACCGA GCTCCCCAAA 
CTCGCGAAAT GCGAATATAG CGAAACCACT AGTTATTTAC TATGGAAAAC CTTAAATCTA 
CGCCTTAAGC ATAGTGATAA CGATATAAAC TGGCGCTCTC TCGTTTCAAT ACTCAATTCT 
GAAGCCTGGG AAAACGAAAA GTACAGAGAC ATACTAAATG GAAGAAAGTG GAGGACCCTG 
GAGTTCGAGA ATGACCACCA CTCTGTGGGT AATATGCATA TTGGAACAGC GTGTACGCGT 
TTATGCTTCC CCTCTGAGAC AATTTATTAC TGTTTTACGT GCTCCACAAA TCCATTGTAT 
GAAATATGTG AGCTTTGTTT CGATAAAGAA AAACATGTAA ATCATTCTTA TGTTGCTAAA 
GTGGTAATGC GCCCAGAAGG CAGAATATGC CATTGTGGTG ACCCCTTTGC CTTTAATGAC 
CCAAGCGACG CGTTCAAGTG CAAGAACGAA TTAAATAATA TTCCTATTTC TAACGACAAT 
AGTAACGTAA CCGATGATGA AAACGTAATC TCCCTATTGA ATTATGTTCT GGATTTTTTA 
ATTGATGTAA CTGTTTCATA CAAAGAGGAG GCAGAAGCCC ATTCAAGCGA GAGAAAAGCA 
TCCTCCCTAA TGCATCCTAA TCAAAACAGC ATTACTGATG ATATAATGGA AAAACACGAA 
TGTGAACCAT TGGTCAATGA TGAAAATTTT GTTTTCTTTG ATAACAATTG GTCCAATACG 
AGAAAAGAAG CACATATGGA GTGGGCTATT CAAATCGAAG AAGAAGAGTG TAACGTACAC 
TACATGGATT TAGCATCTAC AATAACAAGA ATTTTGAATA CACCGGTTGA ATATGCAATC 
TCAATAACGA AGGCTTTAGA AGATTCACAC GATGTAGTGA CTGTGCTACA AAGTGAGAAT 
TTTTTCGAAA TAGATCAAAT AGCCAAGGAG TTTCAAAAGG AAAACATAGT AGTTCATGTA 
AGAAAAGCGG ATGATATATT CAAGAGAAAG TTAACAGATG ATTTAACAGA TTGGTTATAT 
TCTCTGTGCT TTAAGGCGGC CACGTCTTTG CAAAATAAAT ATGCTCTTAG AATTTCCATG 
CTAGATGTTT GGTATTCCCA TTTTTCTAAA ATGAGAGTAT CTCCCACAAA TACCAATCCT 
GACTTTTCTA AGATTAACTT ATTAGGTGGA TTCTTAATAT CTAACGAAGA TAGTGATGAA 
TCATGGTTTA AACCTTGGTC ATTAGAGAAC ATTGAAGACG AAAGAATTTC AAAAATACTC 
ACTAATTACA ATGAAAGGTT GATCAGAGCC CATTCGCCGA ACACAGTAAG CCATTTCTAC 
AATTTCTATG GATCTAGATT CCAATATATT ATCATCAATT CTATTAATAT CCTCTCAAAA 
AAATCTAAAT TCAAAATGTT AAAGATCATG GCGTCATTAT TCTCGTTAAG GGATGAAAGC 
AGAAAGTTTC TTGCCGCACA ATATATTGAT GTTTATCTAT CGGTCTTGTA TGATGCAGTT 
GCATCAGATG CTAAAGAATG TCAGGTAACT CTTATGAGTA TATTAGGGCA ATACACTTTC 
CAAGACCCCA GCATAGCAAA TATGACTATA AGCTCGGGAT TCATTGAACG AACTATTAGA 
TTTGCTTTTA CTCTCATGGC ATTCAATCCA GAAGACTTGA TGTCATATTT GCCCATCTCT 
TTATACAATG GCTTTAAGTT GCCCACCGAA ACAATACGAA ATCGAAGGAC AATTATTTGC 
TTTAAAGACC TTTGTACGAT AATGTCAGCG AACACTGTTC CGGAAGAACT ACTTTCAAAT 
GAGGCAATTT TTAACGCAAT AATTGAGTCC TTCTCTGAAT TCAGTAACGT GCTGCCATTG 
AAAAGGGAAA CTAAAGAACA CGTGGAGGTT GAAAACTTTG ATTTTTCTGC ATTTTATTTT 
TTTTTCTCAT CAATACTAAT AATGACAGAT GGATACACAC GTAGTATATC TTTGGTCAAA 
GATGCGGCTT TCCGTAAGCA AATCGTCCTT AAACTACTTG ATGTTGCTCA AACCAGGGAA 
TTTGAATCAC TAACCAATTC TAGAAAAGCT ATATCACCTG ATAACGCATC AACGAATGAA 
AATGATTCGA ACAAAGCGAC TTTGTCTACA GTCAGAGAGA CGATATGCAA TTATGTCGCA 
GAGACTATTA ATTTCCAGGT AGGTGTAAAT ACACAGTATT TTTTCAATCC AATGTCATAT 
TTGTTCAAAT TTGTTATTCA ATGGAGTCAA TGTGGTAGAT ATGAGCCAAT TCCTGCTTCT 
TTGACAAACT ACATTAATCT CTTCGAAGTT TTTCAAGATA AGCAGAAGGC TTTATATATA 
TCAGAATCTG CTTTGTCAAC CCTAGTCCTA ATCGGGCAAA TAAACGTCGG TTTCTGGGTA 
AGAAACGGAA CTCCAATTAC TCACCAGGCC CGGATGTATA CAAAATACAG TATGCGAGAA 
TTTACTTATA TTAGTGACAT TTTCAATGTG CAATTTAGTA TGGCAATGTG CAATCCTGAT 
GAACTGATGG TTACTTACCT CTCAAGGTGG GGTTTGAAGC ACTGGGCAAA TGGTGTACCT 
ATGTATGATT ATCCTGATAC AGAAACAACT GTAGCCGTTG TTAATGAATG CATTCTGTTA 
TTGATTCAAT TGCTTACTGA AGTGAGATCA TTGGTCATGA AATCATCAAA GGAAGGCTTC 
GAGAGAACGT TCAAATCAGA AATAATTCAT GCCCTTTGCT TTGATACATG CTCTTACGCA 
CAAATTGTTA ACTGTATTCC AGAACATATC ACCAAGCACC CTTCATTTGA TATATATCTC 
GAAAAATACG CCAATTACAC TTCTCCTGTA AGCTTGACTG ACAATGGCAT TTTCGTGTTG 
AAAGAAAAGT ATAAAGATGA GATTGATCCT TATTATATAG GGTTGTCTTC CAGTAGGAGG 
TATGATGTAG AGAAAAATAT CAGGCTGAAT ATGGCAAATT TGAAGAAAAT GAAATATGAA 
GATACTTTTG TACCAGCAAA AAAGGTTAAA GATTTGTTGA AGAATACATT ATTTTCTGGG 
CTGTATTCAA TATCATCAGT GAATACTTTT GGTTTATTTT TGAAAAATAC TTTAGATCAC 
ATTATAAAAT ATGATTATGA CAATTTATTG CCGAGAGTGG TTCATTTGAT TCATTTATGC 
GTGGTTAATA ATCTGAATGA ATTTATGGGG ATACTGTGGC ATGAATACGC AATTGTGGAC 
ACAGAATTCT GTCATTACCA CAGCATTGGT TCTATCCTGT ATTACTGTTT GTTGAAGGAT 
AATTTTTCGG AATCTCATGG TAAGATTAGG GAAATTTTCA GGTATTTAAT GGAAACAGCG 
CCTCACGTAA ATGTAAACAG TTATTTGAGG GAGCAAACCA CGTCATATAC ACCAGGAATA 
TTGTGGCCTA CAAAGGAAGA TAAAAGCCAC AAGGATAAAG AATTTGAAAG GAAAAAACAT 
CTTGCTCGAT TGAGAAAGAA AAAATTAATG AAGAAGTTGG CTCAACAGCA AATGAAATTC 
ATGGAAAACA ATAGTGTAGA TACTTCCGAT ATTAGTACAC CCAGAACCAC ATCACCTAGT 
TTATCTCCCA CTAGAATAAA TGCAGAGAAT AGTAGTAACA CAATCAACAG TTGCTGCGAT 
GATGATTGCG TATTTTGCAA GATGCCGAAG GATGACGATG TATTTGTTTA TTTTTCATAC 
CAAGAACGGA ACATATGTGA TCATGGAATT GATTTTACGA ATCCTACAGA TGTTAATAGA 
ATAAATTCGC TGTTCAGTGG TAAGCAAACA AAAGACAGTG CAATCCAAGA AAACCCCCAG 
GATGATGATG GAACGAGATT GAAATTTACT AGCTGTGAAC CAGTATTAAG AGCATGCGGG 
CATGGGTCTC ATACCAAATG TTTAAGTGGC CATATGAAGT CGATTCGCGG AATACAAAAC 
CAAACTACAA AAAATATTCC ATTATCGTAT GGTTCAGGGT TGATATATTG TCCCGTATGC 
AATTCCTTAA GTAATTCATT TCTACCAAAA ACAAATGATA TAGATAAGAG AACTTCGAGC 
CAGTTTTTTA TGTGTATTGA AAAGCGAAGT GAAGCGGAAG AAAACCTTGA CCCAATGAGC 
TCTATTTGTA TTAAAGCAGC GATGATTCTA GGTGATCTTC AGGGTAAAAA AGTCACGACT 
ATCGAAGATG CATATAAAGT TGTTAATAGT GTTTTTATTA ATACTATTTC AAATACTGAG 
CTCAGATTAA GATCGCACAA AAAGGAAGGA AAAATAGTGA ATATGGAAAG AATATCCAGT 
CAGTGCATTT TAACCTTACA TTTAGTTTGT GAGCTAAAGT CGTTTATTTA CAAAAAATTT 
GTTAATTCAA AAACATTCAG CAGCGAAATC TCTCGTAAAA TATGGAATTG GAATGAGTTT 
CTAATAAAGG GTAATAATGT GAATCTACTT TTGTATATGA GTCAAAATTT TGATAATATT 
GATGGAGGAA AAACCCCGCA ACCGCCTAAC CTATGTATCT ATGAAATGTT CAAAAGAAGG 
TTCCATCAAC TACTACTGTT GCTTGCAAGA GATATGATGA GAGTAAACTT TTACAAAGAC 
TGTCGAAATA AAATCAAAAT ATCATCAAAC GGATCTGAGG AACCATCGAC AAGCTTTTCA 
TATCTATTCA ATACTTTTAA AAAGTATGTT GATCTCTTTA AACCAGACGA CGTTCGGTTT 
GATTTTACAA GTTTAGAGAA AATTAAAGAC TTTATATGCT CTCTATTACT AGAGTCATTG 
TCCATTTTTT GTAGAAGAAC CTTCTTATTA TTCAACATTC AGTATGATGA TGATGGTGAT 
GGTGATAATA ATAATAACCG CAGCAATAAT TTCATGGACG TCAAACAGCG TGAAATAGAG 
CTTATTTTCC GGTATTTTAA ATTACCCAAT TTAACTCACT TTTTGAAGGA TTTTTTCTAC 
AACGAACTAA CACAAAATAT AGAAAGGTAT AATGATGGTA ATGATAATTT GAGAATTCAG 
CAAGTAATAT ATGACATGGT GCAGAACATA AACACAAGAG CATATCCTTC ACCAGAGCAT 
ATCCAGCTTA TCGAACTACC ATTGAACCTT TCCAAATTTT CCCTTGATAA TGATGAAATA 
TCGAATAAAT GTGATAAATA CGAAATCGCA GTATGCTTGT TGTGTGGCCA AAAATGCCAT 
ATTCAAAAAA GCATAGCACT GCAAGGTTAT TTACAGGGGG AATGTACTGA TCATATGAGA 
AATGGATGCG AAATCACCTC CGCTTACGGA GTTTTCTTAA TGACCGGCAC AAATGCGATA 
TATCTGTCAT ACGGCAAAAG AGGTACATTT TATGCTGCAC CTTATCTAAG TAAATACGGA 
GAGACCAACG AAGACTACAA ATTCGGTACT CCCGTCTATT TGAATCGGGC GAGATACGCG 
AATCTAGCGA ATGAGATTGT TTTTGGCAAC ATGATTCCGC ACATAGTGTT CCGACTAACG 
GACGGCAGTG CAGATCTTGG TGGGTGGGAG ACTATGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_013124.1
CDS	1..5619
Translation	MEDSDLSITNIRDFLTELPKLAKCEYSETTSYLLWKTLNLRLKHSDNDINWRSLVSILNSEAWENEKYRDILNGRKWRTLEFENDHHSVGNMHIGTACTRLCFPSETIYYCFTCSTNPLYEICELCFDKEKHVNHSYVAKVVMRPEGRICHCGDPFAFNDPSDAFKCKNELNNIPISNDNSNVTDDENVISLLNYVLDFLIDVTVSYKEEAEAHSSERKASSLMHPNQNSITDDIMEKHECEPLVNDENFVFFDNNWSNTRKEAHMEWAIQIEEEECNVHYMDLASTITRILNTPVEYAISITKALEDSHDVVTVLQSENFFEIDQIAKEFQKENIVVHVRKADDIFKRKLTDDLTDWLYSLCFKAATSLQNKYALRISMLDVWYSHFSKMRVSPTNTNPDFSKINLLGGFLISNEDSDESWFKPWSLENIEDERISKILTNYNERLIRAHSPNTVSHFYNFYGSRFQYIIINSINILSKKSKFKMLKIMASLFSLRDESRKFLAAQYIDVYLSVLYDAVASDAKECQVTLMSILGQYTFQDPSIANMTISSGFIERTIRFAFTLMAFNPEDLMSYLPISLYNGFKLPTETIRNRRTIICFKDLCTIMSANTVPEELLSNEAIFNAIIESFSEFSNVLPLKRETKEHVEVENFDFSAFYFFFSSILIMTDGYTRSISLVKDAAFRKQIVLKLLDVAQTREFESLTNSRKAISPDNASTNENDSNKATLSTVRETICNYVAETINFQVGVNTQYFFNPMSYLFKFVIQWSQCGRYEPIPASLTNYINLFEVFQDKQKALYISESALSTLVLIGQINVGFWVRNGTPITHQARMYTKYSMREFTYISDIFNVQFSMAMCNPDELMVTYLSRWGLKHWANGVPMYDYPDTETTVAVVNECILLLIQLLTEVRSLVMKSSKEGFERTFKSEIIHALCFDTCSYAQIVNCIPEHITKHPSFDIYLEKYANYTSPVSLTDNGIFVLKEKYKDEIDPYYIGLSSSRRYDVEKNIRLNMANLKKMKYEDTFVPAKKVKDLLKNTLFSGLYSISSVNTFGLFLKNTLDHIIKYDYDNLLPRVVHLIHLCVVNNLNEFMGILWHEYAIVDTEFCHYHSIGSILYYCLLKDNFSESHGKIREIFRYLMETAPHVNVNSYLREQTTSYTPGILWPTKEDKSHKDKEFERKKHLARLRKKKLMKKLAQQQMKFMENNSVDTSDISTPRTTSPSLSPTRINAENSSNTINSCCDDDCVFCKMPKDDDVFVYFSYQERNICDHGIDFTNPTDVNRINSLFSGKQTKDSAIQENPQDDDGTRLKFTSCEPVLRACGHGSHTKCLSGHMKSIRGIQNQTTKNIPLSYGSGLIYCPVCNSLSNSFLPKTNDIDKRTSSQFFMCIEKRSEAEENLDPMSSICIKAAMILGDLQGKKVTTIEDAYKVVNSVFINTISNTELRLRSHKKEGKIVNMERISSQCILTLHLVCELKSFIYKKFVNSKTFSSEISRKIWNWNEFLIKGNNVNLLLYMSQNFDNIDGGKTPQPPNLCIYEMFKRRFHQLLLLLARDMMRVNFYKDCRNKIKISSNGSEEPSTSFSYLFNTFKKYVDLFKPDDVRFDFTSLEKIKDFICSLLLESLSIFCRRTFLLFNIQYDDDGDGDNNNNRSNNFMDVKQREIELIFRYFKLPNLTHFLKDFFYNELTQNIERYNDGNDNLRIQQVIYDMVQNINTRAYPSPEHIQLIELPLNLSKFSLDNDEISNKCDKYEIAVCLLCGQKCHIQKSIALQGYLQGECTDHMRNGCEITSAYGVFLMTGTNAIYLSYGKRGTFYAAPYLSKYGETNEDYKFGTPVYLNRARYANLANEIVFGNMIPHIVFRLTDGSADLGGWETM

Target ORF information:

RefSeq Version	NM_001181911.2
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C putative ubiquitin-protein ligase UBR2 (UBR2), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001181911.2

ORF Insert Sequence:

ATGGAAGATT CTGACCTGTC TATAACCAAT ATAAGAGATT TTCTTACCGA GCTCCCCAAA 
CTCGCGAAAT GCGAATATAG CGAAACCACT AGTTATTTAC TATGGAAAAC CTTAAATCTA 
CGCCTTAAGC ATAGTGATAA CGATATAAAC TGGCGCTCTC TCGTTTCAAT ACTCAATTCT 
GAAGCCTGGG AAAACGAAAA GTACAGAGAC ATACTAAATG GAAGAAAGTG GAGGACCCTG 
GAGTTCGAGA ATGACCACCA CTCTGTGGGT AATATGCATA TTGGAACAGC GTGTACGCGT 
TTATGCTTCC CCTCTGAGAC AATTTATTAC TGTTTTACGT GCTCCACAAA TCCATTGTAT 
GAAATATGTG AGCTTTGTTT CGATAAAGAA AAACATGTAA ATCATTCTTA TGTTGCTAAA 
GTGGTAATGC GCCCAGAAGG CAGAATATGC CATTGTGGTG ACCCCTTTGC CTTTAATGAC 
CCAAGCGACG CGTTCAAGTG CAAGAACGAA TTAAATAATA TTCCTATTTC TAACGACAAT 
AGTAACGTAA CCGATGATGA AAACGTAATC TCCCTATTGA ATTATGTTCT GGATTTTTTA 
ATTGATGTAA CTGTTTCATA CAAAGAGGAG GCAGAAGCCC ATTCAAGCGA GAGAAAAGCA 
TCCTCCCTAA TGCATCCTAA TCAAAACAGC ATTACTGATG ATATAATGGA AAAACACGAA 
TGTGAACCAT TGGTCAATGA TGAAAATTTT GTTTTCTTTG ATAACAATTG GTCCAATACG 
AGAAAAGAAG CACATATGGA GTGGGCTATT CAAATCGAAG AAGAAGAGTG TAACGTACAC 
TACATGGATT TAGCATCTAC AATAACAAGA ATTTTGAATA CACCGGTTGA ATATGCAATC 
TCAATAACGA AGGCTTTAGA AGATTCACAC GATGTAGTGA CTGTGCTACA AAGTGAGAAT 
TTTTTCGAAA TAGATCAAAT AGCCAAGGAG TTTCAAAAGG AAAACATAGT AGTTCATGTA 
AGAAAAGCGG ATGATATATT CAAGAGAAAG TTAACAGATG ATTTAACAGA TTGGTTATAT 
TCTCTGTGCT TTAAGGCGGC CACGTCTTTG CAAAATAAAT ATGCTCTTAG AATTTCCATG 
CTAGATGTTT GGTATTCCCA TTTTTCTAAA ATGAGAGTAT CTCCCACAAA TACCAATCCT 
GACTTTTCTA AGATTAACTT ATTAGGTGGA TTCTTAATAT CTAACGAAGA TAGTGATGAA 
TCATGGTTTA AACCTTGGTC ATTAGAGAAC ATTGAAGACG AAAGAATTTC AAAAATACTC 
ACTAATTACA ATGAAAGGTT GATCAGAGCC CATTCGCCGA ACACAGTAAG CCATTTCTAC 
AATTTCTATG GATCTAGATT CCAATATATT ATCATCAATT CTATTAATAT CCTCTCAAAA 
AAATCTAAAT TCAAAATGTT AAAGATCATG GCGTCATTAT TCTCGTTAAG GGATGAAAGC 
AGAAAGTTTC TTGCCGCACA ATATATTGAT GTTTATCTAT CGGTCTTGTA TGATGCAGTT 
GCATCAGATG CTAAAGAATG TCAGGTAACT CTTATGAGTA TATTAGGGCA ATACACTTTC 
CAAGACCCCA GCATAGCAAA TATGACTATA AGCTCGGGAT TCATTGAACG AACTATTAGA 
TTTGCTTTTA CTCTCATGGC ATTCAATCCA GAAGACTTGA TGTCATATTT GCCCATCTCT 
TTATACAATG GCTTTAAGTT GCCCACCGAA ACAATACGAA ATCGAAGGAC AATTATTTGC 
TTTAAAGACC TTTGTACGAT AATGTCAGCG AACACTGTTC CGGAAGAACT ACTTTCAAAT 
GAGGCAATTT TTAACGCAAT AATTGAGTCC TTCTCTGAAT TCAGTAACGT GCTGCCATTG 
AAAAGGGAAA CTAAAGAACA CGTGGAGGTT GAAAACTTTG ATTTTTCTGC ATTTTATTTT 
TTTTTCTCAT CAATACTAAT AATGACAGAT GGATACACAC GTAGTATATC TTTGGTCAAA 
GATGCGGCTT TCCGTAAGCA AATCGTCCTT AAACTACTTG ATGTTGCTCA AACCAGGGAA 
TTTGAATCAC TAACCAATTC TAGAAAAGCT ATATCACCTG ATAACGCATC AACGAATGAA 
AATGATTCGA ACAAAGCGAC TTTGTCTACA GTCAGAGAGA CGATATGCAA TTATGTCGCA 
GAGACTATTA ATTTCCAGGT AGGTGTAAAT ACACAGTATT TTTTCAATCC AATGTCATAT 
TTGTTCAAAT TTGTTATTCA ATGGAGTCAA TGTGGTAGAT ATGAGCCAAT TCCTGCTTCT 
TTGACAAACT ACATTAATCT CTTCGAAGTT TTTCAAGATA AGCAGAAGGC TTTATATATA 
TCAGAATCTG CTTTGTCAAC CCTAGTCCTA ATCGGGCAAA TAAACGTCGG TTTCTGGGTA 
AGAAACGGAA CTCCAATTAC TCACCAGGCC CGGATGTATA CAAAATACAG TATGCGAGAA 
TTTACTTATA TTAGTGACAT TTTCAATGTG CAATTTAGTA TGGCAATGTG CAATCCTGAT 
GAACTGATGG TTACTTACCT CTCAAGGTGG GGTTTGAAGC ACTGGGCAAA TGGTGTACCT 
ATGTATGATT ATCCTGATAC AGAAACAACT GTAGCCGTTG TTAATGAATG CATTCTGTTA 
TTGATTCAAT TGCTTACTGA AGTGAGATCA TTGGTCATGA AATCATCAAA GGAAGGCTTC 
GAGAGAACGT TCAAATCAGA AATAATTCAT GCCCTTTGCT TTGATACATG CTCTTACGCA 
CAAATTGTTA ACTGTATTCC AGAACATATC ACCAAGCACC CTTCATTTGA TATATATCTC 
GAAAAATACG CCAATTACAC TTCTCCTGTA AGCTTGACTG ACAATGGCAT TTTCGTGTTG 
AAAGAAAAGT ATAAAGATGA GATTGATCCT TATTATATAG GGTTGTCTTC CAGTAGGAGG 
TATGATGTAG AGAAAAATAT CAGGCTGAAT ATGGCAAATT TGAAGAAAAT GAAATATGAA 
GATACTTTTG TACCAGCAAA AAAGGTTAAA GATTTGTTGA AGAATACATT ATTTTCTGGG 
CTGTATTCAA TATCATCAGT GAATACTTTT GGTTTATTTT TGAAAAATAC TTTAGATCAC 
ATTATAAAAT ATGATTATGA CAATTTATTG CCGAGAGTGG TTCATTTGAT TCATTTATGC 
GTGGTTAATA ATCTGAATGA ATTTATGGGG ATACTGTGGC ATGAATACGC AATTGTGGAC 
ACAGAATTCT GTCATTACCA CAGCATTGGT TCTATCCTGT ATTACTGTTT GTTGAAGGAT 
AATTTTTCGG AATCTCATGG TAAGATTAGG GAAATTTTCA GGTATTTAAT GGAAACAGCG 
CCTCACGTAA ATGTAAACAG TTATTTGAGG GAGCAAACCA CGTCATATAC ACCAGGAATA 
TTGTGGCCTA CAAAGGAAGA TAAAAGCCAC AAGGATAAAG AATTTGAAAG GAAAAAACAT 
CTTGCTCGAT TGAGAAAGAA AAAATTAATG AAGAAGTTGG CTCAACAGCA AATGAAATTC 
ATGGAAAACA ATAGTGTAGA TACTTCCGAT ATTAGTACAC CCAGAACCAC ATCACCTAGT 
TTATCTCCCA CTAGAATAAA TGCAGAGAAT AGTAGTAACA CAATCAACAG TTGCTGCGAT 
GATGATTGCG TATTTTGCAA GATGCCGAAG GATGACGATG TATTTGTTTA TTTTTCATAC 
CAAGAACGGA ACATATGTGA TCATGGAATT GATTTTACGA ATCCTACAGA TGTTAATAGA 
ATAAATTCGC TGTTCAGTGG TAAGCAAACA AAAGACAGTG CAATCCAAGA AAACCCCCAG 
GATGATGATG GAACGAGATT GAAATTTACT AGCTGTGAAC CAGTATTAAG AGCATGCGGG 
CATGGGTCTC ATACCAAATG TTTAAGTGGC CATATGAAGT CGATTCGCGG AATACAAAAC 
CAAACTACAA AAAATATTCC ATTATCGTAT GGTTCAGGGT TGATATATTG TCCCGTATGC 
AATTCCTTAA GTAATTCATT TCTACCAAAA ACAAATGATA TAGATAAGAG AACTTCGAGC 
CAGTTTTTTA TGTGTATTGA AAAGCGAAGT GAAGCGGAAG AAAACCTTGA CCCAATGAGC 
TCTATTTGTA TTAAAGCAGC GATGATTCTA GGTGATCTTC AGGGTAAAAA AGTCACGACT 
ATCGAAGATG CATATAAAGT TGTTAATAGT GTTTTTATTA ATACTATTTC AAATACTGAG 
CTCAGATTAA GATCGCACAA AAAGGAAGGA AAAATAGTGA ATATGGAAAG AATATCCAGT 
CAGTGCATTT TAACCTTACA TTTAGTTTGT GAGCTAAAGT CGTTTATTTA CAAAAAATTT 
GTTAATTCAA AAACATTCAG CAGCGAAATC TCTCGTAAAA TATGGAATTG GAATGAGTTT 
CTAATAAAGG GTAATAATGT GAATCTACTT TTGTATATGA GTCAAAATTT TGATAATATT 
GATGGAGGAA AAACCCCGCA ACCGCCTAAC CTATGTATCT ATGAAATGTT CAAAAGAAGG 
TTCCATCAAC TACTACTGTT GCTTGCAAGA GATATGATGA GAGTAAACTT TTACAAAGAC 
TGTCGAAATA AAATCAAAAT ATCATCAAAC GGATCTGAGG AACCATCGAC AAGCTTTTCA 
TATCTATTCA ATACTTTTAA AAAGTATGTT GATCTCTTTA AACCAGACGA CGTTCGGTTT 
GATTTTACAA GTTTAGAGAA AATTAAAGAC TTTATATGCT CTCTATTACT AGAGTCATTG 
TCCATTTTTT GTAGAAGAAC CTTCTTATTA TTCAACATTC AGTATGATGA TGATGGTGAT 
GGTGATAATA ATAATAACCG CAGCAATAAT TTCATGGACG TCAAACAGCG TGAAATAGAG 
CTTATTTTCC GGTATTTTAA ATTACCCAAT TTAACTCACT TTTTGAAGGA TTTTTTCTAC 
AACGAACTAA CACAAAATAT AGAAAGGTAT AATGATGGTA ATGATAATTT GAGAATTCAG 
CAAGTAATAT ATGACATGGT GCAGAACATA AACACAAGAG CATATCCTTC ACCAGAGCAT 
ATCCAGCTTA TCGAACTACC ATTGAACCTT TCCAAATTTT CCCTTGATAA TGATGAAATA 
TCGAATAAAT GTGATAAATA CGAAATCGCA GTATGCTTGT TGTGTGGCCA AAAATGCCAT 
ATTCAAAAAA GCATAGCACT GCAAGGTTAT TTACAGGGGG AATGTACTGA TCATATGAGA 
AATGGATGCG AAATCACCTC CGCTTACGGA GTTTTCTTAA TGACCGGCAC AAATGCGATA 
TATCTGTCAT ACGGCAAAAG AGGTACATTT TATGCTGCAC CTTATCTAAG TAAATACGGA 
GAGACCAACG AAGACTACAA ATTCGGTACT CCCGTCTATT TGAATCGGGC GAGATACGCG 
AATCTAGCGA ATGAGATTGT TTTTGGCAAC ATGATTCCGC ACATAGTGTT CCGACTAACG 
GACGGCAGTG CAGATCTTGG TGGGTGGGAG ACTATGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XII.
Nature387(6632 Suppl)87-90(1997 May)
Johnston M,Hillier L,Riles L,Albermann K,Andr? B,Ansorge W,Benes V,Br?ckner M,Delius H,Dubois E,D?sterh?ft A,Entian KD,Floeth M,Goffeau A,Hebling U,Heumann K,Heuss-Neitzel D,Hilbert H,Hilger F,Kleine K,K?tter P,Louis EJ,Messenguy F,Mewes HW,Hoheisel JD

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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UBR2 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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