SPA2 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	SPA2
Entrez Gene ID	850639
Full Name	Spa2p
Synonyms	FUS6,PEA1,PPF1
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001181841.1	NP_013079.1	Spa2p

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XII.
Johnston M, Hillier L, Riles L, Albermann K, Andr? B, Ansorge W, Benes V, Br?ckner M, Delius H, Dubois E, D?sterh?ft A, Entian KD, Floeth M, Goffeau A, Hebling U, Heumann K, Heuss-Neitzel D, Hilbert H, Hilger F, Kleine K, K?tter P, Louis EJ, Messenguy F, Mewes HW, Hoheisel JD
Nature387(6632 Suppl)87-90(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

the pea2-M409L mutation results in decreased invasive filamentation and elongation, diminished activity of a Kss1p MAPK pathway reporter, decreased unipolar budding, and diminished binding of the polarisome protein Spa2p.
Title: Pooled segregant sequencing reveals genetic determinants of yeast pseudohyphal growth.

findings show Cdc42 serves an essential function in the establishment of bud site by catalyzing translocation of bud-site components such as Spa2 to the cell cortex; role of Cdc42 in the cortical localization of Spa2 appears to be independent of Cdc24
Title: Cdc42-dependent localization of polarisome component Spa2 to the incipient bud site is independent of the GDP/GTP exchange factor Cdc24.

Spa2p is involved in cytokinesis and cell wall morphogenesis.
Title: Cell polarity protein Spa2P associates with proteins involved in actin function in Saccharomyces cerevisiae.

Therefore, we suggest that Mid1 function is influenced by Spa2 function through polarized morphogenesis.
Title: Polarized morphogenesis regulator Spa2 is required for the function of putative stretch-activated Ca2+-permeable channel component Mid1 in Saccharomyces cerevisiae.

The following SPA2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SPA2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi00311	NM_001181841.1 Latest version!	Saccharomyces cerevisiae S288C Spa2p (SPA2), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SPA2 ( NM_001181841.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_013079.1 Saccharomyces cerevisiae S288C Spa2p (SPA2), partial mRNA.

Price & Availability↑

CloneID	OSi00311
Clone ID Related Accession (Same CDS sequence)	NM_001181841.1
Accession Version	NM_001181841.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4401bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	Spa2p
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001144). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGGGTACGT CAAGCGAGGT TTCTCTCGCA CATCATAGAG ATATCTTCCA TTACTACGTC 
TCACTGAAGA CTTTTTTCGA GGTGACTGGC GAAAATCGTG ACAGGTCAAA TTCGACACGA 
GCTCAAAAGG CCAGAGCCAA GCTGTTGAAG CTATCTTCTT CGCAATTTTA CGAGCTGAGT 
ACAGACGTGT CCGATGAGCT GCAGAGGAGA ATCGGTGAAG ATGCTAACCA ACCAGATTAC 
CTTTTGCCGA AGGCAAATTT CCACATGAAA AGGAACCAGG CTAGACAGAA ACTGGCCAAT 
CTATCACAAA CTCGATTTAA TGATTTGTTG GACGATATCC TTTTTGAGAT CAAGAGAAGA 
GGGTTCGACA AGGATTTGGA TGCTCCACGG CCCCCATTAC CGCAGCCGAT GAAACAAGAG 
GTCAGCAAAG ACAGCGATGA TACTGCAAGA ACATCCACAA ATTCTTCCTC TGTGACTCAA 
GTAGCTCCAA ACGTCTCCGT ACAACCTTCT TTGGTCATTC CTAAGATGGC ATCTATCGAT 
TGGTCTTCTG AGGAAGAAGA AGAGGAGCAA GTAAAGGAGA AGCCAAATGA ACCGGAGGGA 
AAACAAACAA GCATGGATGA AAAGAAAGAG GCTAAACCTG CTCTAAACCC CATAGTTACA 
GATTCTGATC TGCCTGACTC CCAAGTGCTC GCTCGTGATA TCACATCAAT GGCAAGGACT 
CCAACAACGA CGCATAAAAA TTACTGGGAC GTTAATGATT CTCCAATTAT CAAGGTAGAT 
AAAGATATCG ATAACGAAAA GGGTCCCGAA CAGTTGAAGA GCCCTGAAGT ACAACGGGCT 
GAGAACAATA ACCCTAACTC AGAGATGGAA GACAAGGTTA AAGAACTGAC TGATTTAAAC 
AGCGACTTAC ATTTGCAAAT TGAAGATTTG AATGCTAAGT TAGCATCTTT AACCAGCGAG 
AAGGAAAAGG AGAAGAAGGA AGAGAAGGAG GAAAAAGAAA AGGAAAAGAA CTTAAAGATT 
AACTACACCA TTGATGAAAG TTTTCAGAAA GAATTGCTGT CATTAAACTC TCAAATCGGT 
GAATTATCAA TTGAGAATGA AAATTTGAAG CAGAAAATTT CAGAATTCGA ACTGCATCAA 
AAAAAGAATG ACAACCATAA TGATTTGAAA ATCACTGACG GTTTTATTAG CAAGTACTCT 
TCTGCCGATG GGCTCATTCC AGCTCAATAC ATCTTAAACG CTAACAACTT GATAATTCAA 
TTTACTACTA GGCTTTCCGC AGTACCCATA GGCGACTCCA CGGCAATTTC CCATCAAATT 
GGCGAAGAGT TATTTCAAAT ATTATCCCAG TTATCGAACC TAATCTCCCA GCTATTACTA 
TCGGCCGACC TATTACAGTA CAAAGATCAG GTCATTTTAC TGAAGGCATC ATTATCGCAT 
GCGATCACAT CGATAAGATA TTTCTCTGTT TACGGTCCCG TATTAATTCC GAAAATAACT 
GTGCAAGCTG CTGTTTCAGA GGTTTGTTTT GCCATGTGTA ATCTAATTGA TTCAGCGAAA 
ATAAAATCCG ATTCAAATGG TGAGAGCACC ACCTCTAATG AAGGTAACCG ACAGGTATTA 
GAATATTCTT CACCAACTGC TACCACCCCA ATGACGCCAA CTTTCCCCTC GACTTCTGGA 
ATAAATATGA AGAAGGGGTT TATAAACCCA AGAAAACCAG CATCTTTCTT GAATGATGTG 
GAGGAAGAAG AATCTCCAGT CAAGCCATTG AAAATTACAC AAAAGGCAAT TAACAGTCCG 
ATCATAAGAC CGTCATCGTC TAATGGAGTT CCAACAACCT CAAGAAAACC TTCAGGAACG 
GGGCTATTTA GTTTAATGAT TGATTCATCA ATTGCTAAGA ATAGCTCCCA TAAAGAGGAT 
AATGATAAAT ATGTCTCGCC CATAAAGGCA GTAACATCGG CCTCCAATTC TGCAAGTAGC 
AATATTTCCG AAATTCCTAA ACTAACACTA CCTCCACAAG CCAAAATCGG TACTGTTATT 
CCACCGTCAG AGAATCAAGT TCCCAATATT AAAATCGAGA ATACAGAAGA GGATAATAAA 
AGGAGTGACA TAACAAATGA AATCTCTGTT AAACCAACTT CTAGCATTGC TGATAAACTG 
AAACAATTTG AGCAAAGTTC CGAAAAGAAA TCATCACCAA AGGAAAATCC TATAGCAAAA 
GAAGAAATGG ATTCAAAACC AAAACTATCC AATAAATTTA TCACTTCAAT GAATGATGTG 
TCCACAGATG ATTCAAGCTC TGATGGTAAC GAAAATGACG ATGCAGACGA TGATGATGAT 
TTTACCTATA TGGCATTGAA ACAAACAATG AAGAGAGAAG GTTCAAAAAT TGAAAAAAAT 
AATGACAGCA AACTACCTGC AAATATAGTG GAACTTGATT TACATGAGTC ACCGGAGTCC 
GTGAAGATTG AATCTCCTGA ATCGATAAAG GAAATCACGT CATCTGAAAT GTCTTCAGAA 
ATGCCAAGTA GTTCGCTGCC TAAGAGATTA GTAGAGGATG TTGAGCCTTC AGAAATGCCA 
GAGAAGGGCG CATCTGTAGA ATCAGTCAGG AAGAAAAATT TTCAAGAACC ACTTGGTAAT 
GTCGAATCTC CGGATATGAC GCAGAAGGTC AAGTCTTTGG GTATGACAGG AAAGGCTGTA 
GGCCCAGAAT CAGATAGTAG GGTCGAATCT CCGGGCATGA CAGGACAGAT TAAATCTTTG 
AATATGGCAG GAAAAGTTGT AGGCCCAGAA GCAGATAGTA GGGTCGAATC TCCGGGCATG 
AAAGAGCAGA TTAAGTCTTT GGGTATGACA GGAAAAATTA CAGCTCAAGA ATCAATCAAG 
TCCCCGGAAG CGGCTAGGAA GTTGGCGTCA TCAGGAGAAG TTGACAAAAT TGAATCTCCA 
AGAATGGTAA GGGAAAGCGA GTCCTTGGAG GCAGTAGGCA ATACTATCCC CTCAAACATG 
ACAGTGAAAA TGGAATCCCC AAATTTAAAG GGAAATACTG TGTCTGAACC TCAAGAAATA 
AGGAGAGACA TTGCCTCCTC AGAGCCGATA GAGAATGTTG ACCCCCCAAA AGTACTAAAA 
AAGATTGTCT TTCCAAAGGC TGTTAATAGA ACTGGATCAC CAAAATCAGT CGAAAAGACT 
CCATCTTCAG CTACACTGAA AAAGAGCGGG CTCCCAGAAC CGAATAGCCA AATTGTTTCT 
CCTGAATTGG CAAAAAATTC ACCTCTAGCA CCGATAAAGA AAAATGTCGA GTTACGAGAA 
ACTAATAAAC CACATACTGA GACTATCACT TCTGTGGAAC CAACAAACAA GGATGCCAAT 
ACTTCTTGGA GAGACGCCGA CTTAAACCGT ACGATCAAAC GAGAGGAGGA GGACGAAGAT 
TTTGATAGAG TGAACCACAA TATCCAGATC ACTGGTGCAT ATACGAAAAC TGGAAAAATT 
GATTATCATA AAATACCTGT TGATCGTAAA GCAAAAAGCG AAGCAGAAGT GCATACTTCC 
GAGGAAGATA TTGATGAATC AAATAATGTT AATGGAAAAA GAGCTGATGC CCAAATACAC 
ATCACTGAAA GAAAGCATGC TTTCGTAAAT CCAACTGAAA ATTCACAGGT AAAAAAGACG 
AGCCACTCAC CATTTTTAAA CAGTAAACCG GTTCAATACG AGAACTCAGA GTCGAACGGC 
GGCATTAACA ACCACATAAA GATAAAAAAT ACTGGAGAAA CTACGGCACA TGACGAGAAA 
CATTATAGTG ATGATGATGA TTCTAGCTAT CAATTTGTTC CCATGAAACA TGAAGAACAA 
GAACAAGAAC AAAACAGAAG TGAGGAAGAG GAAAGTGAAG ATGACGATGA AGAGGAAGAA 
GACAGTGATT TTGATGTGGA TACATTTGAC ATTGAAAATC CGGATAATAC ACTATCAGAG 
TTACTATTGT ATTTAGAACA TCAAACAATG GACGTCATAT CCACGATTCA ATCGCTTTTG 
ACATCGATCA AGAAACCACA GGTGACGAAG GGTAATTTGA GGGGAGAATC GAATGCAATC 
AACCAAGTCA TAGGTCAAAT GGTGGACGCT ACTAGCATAT CAATGGAGCA AAGCAGAAAT 
GCCAATTTGA AGAAACACGG TGATTGGGTG GTGCAAAGTC TAAGAGACTG TTCGCGTAGA 
ATGACAATTT TGTGCCAATT AACTGGCGAT GGAATACTAG CGAAGGAAAA GAGCGATCAA 
GATTATGCTG ACAAAAACTT CAAACAGCGG TTGGCAGGGA TTGCGTTTGA TGTTGCCAAA 
TGTACAAAGG AGCTGGTAAA AACTGTAGAA GAGGCAAGTT TGAAGGACGA AATAAATTAT 
TTGAATTCGA AGTTGAAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_013079.1
CDS	1..4401
Translation	MGTSSEVSLAHHRDIFHYYVSLKTFFEVTGENRDRSNSTRAQKARAKLLKLSSSQFYELSTDVSDELQRRIGEDANQPDYLLPKANFHMKRNQARQKLANLSQTRFNDLLDDILFEIKRRGFDKDLDAPRPPLPQPMKQEVSKDSDDTARTSTNSSSVTQVAPNVSVQPSLVIPKMASIDWSSEEEEEEQVKEKPNEPEGKQTSMDEKKEAKPALNPIVTDSDLPDSQVLARDITSMARTPTTTHKNYWDVNDSPIIKVDKDIDNEKGPEQLKSPEVQRAENNNPNSEMEDKVKELTDLNSDLHLQIEDLNAKLASLTSEKEKEKKEEKEEKEKEKNLKINYTIDESFQKELLSLNSQIGELSIENENLKQKISEFELHQKKNDNHNDLKITDGFISKYSSADGLIPAQYILNANNLIIQFTTRLSAVPIGDSTAISHQIGEELFQILSQLSNLISQLLLSADLLQYKDQVILLKASLSHAITSIRYFSVYGPVLIPKITVQAAVSEVCFAMCNLIDSAKIKSDSNGESTTSNEGNRQVLEYSSPTATTPMTPTFPSTSGINMKKGFINPRKPASFLNDVEEEESPVKPLKITQKAINSPIIRPSSSNGVPTTSRKPSGTGLFSLMIDSSIAKNSSHKEDNDKYVSPIKAVTSASNSASSNISEIPKLTLPPQAKIGTVIPPSENQVPNIKIENTEEDNKRSDITNEISVKPTSSIADKLKQFEQSSEKKSSPKENPIAKEEMDSKPKLSNKFITSMNDVSTDDSSSDGNENDDADDDDDFTYMALKQTMKREGSKIEKNNDSKLPANIVELDLHESPESVKIESPESIKEITSSEMSSEMPSSSLPKRLVEDVEPSEMPEKGASVESVRKKNFQEPLGNVESPDMTQKVKSLGMTGKAVGPESDSRVESPGMTGQIKSLNMAGKVVGPEADSRVESPGMKEQIKSLGMTGKITAQESIKSPEAARKLASSGEVDKIESPRMVRESESLEAVGNTIPSNMTVKMESPNLKGNTVSEPQEIRRDIASSEPIENVDPPKVLKKIVFPKAVNRTGSPKSVEKTPSSATLKKSGLPEPNSQIVSPELAKNSPLAPIKKNVELRETNKPHTETITSVEPTNKDANTSWRDADLNRTIKREEEDEDFDRVNHNIQITGAYTKTGKIDYHKIPVDRKAKSEAEVHTSEEDIDESNNVNGKRADAQIHITERKHAFVNPTENSQVKKTSHSPFLNSKPVQYENSESNGGINNHIKIKNTGETTAHDEKHYSDDDDSSYQFVPMKHEEQEQEQNRSEEEESEDDDEEEEDSDFDVDTFDIENPDNTLSELLLYLEHQTMDVISTIQSLLTSIKKPQVTKGNLRGESNAINQVIGQMVDATSISMEQSRNANLKKHGDWVVQSLRDCSRRMTILCQLTGDGILAKEKSDQDYADKNFKQRLAGIAFDVAKCTKELVKTVEEASLKDEINYLNSKLK

Target ORF information:

RefSeq Version	NM_001181841.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C Spa2p (SPA2), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001181841.1

ORF Insert Sequence:

ATGGGTACGT CAAGCGAGGT TTCTCTCGCA CATCATAGAG ATATCTTCCA TTACTACGTC 
TCACTGAAGA CTTTTTTCGA GGTGACTGGC GAAAATCGTG ACAGGTCAAA TTCGACACGA 
GCTCAAAAGG CCAGAGCCAA GCTGTTGAAG CTATCTTCTT CGCAATTTTA CGAGCTGAGT 
ACAGACGTGT CCGATGAGCT GCAGAGGAGA ATCGGTGAAG ATGCTAACCA ACCAGATTAC 
CTTTTGCCGA AGGCAAATTT CCACATGAAA AGGAACCAGG CTAGACAGAA ACTGGCCAAT 
CTATCACAAA CTCGATTTAA TGATTTGTTG GACGATATCC TTTTTGAGAT CAAGAGAAGA 
GGGTTCGACA AGGATTTGGA TGCTCCACGG CCCCCATTAC CGCAGCCGAT GAAACAAGAG 
GTCAGCAAAG ACAGCGATGA TACTGCAAGA ACATCCACAA ATTCTTCCTC TGTGACTCAA 
GTAGCTCCAA ACGTCTCCGT ACAACCTTCT TTGGTCATTC CTAAGATGGC ATCTATCGAT 
TGGTCTTCTG AGGAAGAAGA AGAGGAGCAA GTAAAGGAGA AGCCAAATGA ACCGGAGGGA 
AAACAAACAA GCATGGATGA AAAGAAAGAG GCTAAACCTG CTCTAAACCC CATAGTTACA 
GATTCTGATC TGCCTGACTC CCAAGTGCTC GCTCGTGATA TCACATCAAT GGCAAGGACT 
CCAACAACGA CGCATAAAAA TTACTGGGAC GTTAATGATT CTCCAATTAT CAAGGTAGAT 
AAAGATATCG ATAACGAAAA GGGTCCCGAA CAGTTGAAGA GCCCTGAAGT ACAACGGGCT 
GAGAACAATA ACCCTAACTC AGAGATGGAA GACAAGGTTA AAGAACTGAC TGATTTAAAC 
AGCGACTTAC ATTTGCAAAT TGAAGATTTG AATGCTAAGT TAGCATCTTT AACCAGCGAG 
AAGGAAAAGG AGAAGAAGGA AGAGAAGGAG GAAAAAGAAA AGGAAAAGAA CTTAAAGATT 
AACTACACCA TTGATGAAAG TTTTCAGAAA GAATTGCTGT CATTAAACTC TCAAATCGGT 
GAATTATCAA TTGAGAATGA AAATTTGAAG CAGAAAATTT CAGAATTCGA ACTGCATCAA 
AAAAAGAATG ACAACCATAA TGATTTGAAA ATCACTGACG GTTTTATTAG CAAGTACTCT 
TCTGCCGATG GGCTCATTCC AGCTCAATAC ATCTTAAACG CTAACAACTT GATAATTCAA 
TTTACTACTA GGCTTTCCGC AGTACCCATA GGCGACTCCA CGGCAATTTC CCATCAAATT 
GGCGAAGAGT TATTTCAAAT ATTATCCCAG TTATCGAACC TAATCTCCCA GCTATTACTA 
TCGGCCGACC TATTACAGTA CAAAGATCAG GTCATTTTAC TGAAGGCATC ATTATCGCAT 
GCGATCACAT CGATAAGATA TTTCTCTGTT TACGGTCCCG TATTAATTCC GAAAATAACT 
GTGCAAGCTG CTGTTTCAGA GGTTTGTTTT GCCATGTGTA ATCTAATTGA TTCAGCGAAA 
ATAAAATCCG ATTCAAATGG TGAGAGCACC ACCTCTAATG AAGGTAACCG ACAGGTATTA 
GAATATTCTT CACCAACTGC TACCACCCCA ATGACGCCAA CTTTCCCCTC GACTTCTGGA 
ATAAATATGA AGAAGGGGTT TATAAACCCA AGAAAACCAG CATCTTTCTT GAATGATGTG 
GAGGAAGAAG AATCTCCAGT CAAGCCATTG AAAATTACAC AAAAGGCAAT TAACAGTCCG 
ATCATAAGAC CGTCATCGTC TAATGGAGTT CCAACAACCT CAAGAAAACC TTCAGGAACG 
GGGCTATTTA GTTTAATGAT TGATTCATCA ATTGCTAAGA ATAGCTCCCA TAAAGAGGAT 
AATGATAAAT ATGTCTCGCC CATAAAGGCA GTAACATCGG CCTCCAATTC TGCAAGTAGC 
AATATTTCCG AAATTCCTAA ACTAACACTA CCTCCACAAG CCAAAATCGG TACTGTTATT 
CCACCGTCAG AGAATCAAGT TCCCAATATT AAAATCGAGA ATACAGAAGA GGATAATAAA 
AGGAGTGACA TAACAAATGA AATCTCTGTT AAACCAACTT CTAGCATTGC TGATAAACTG 
AAACAATTTG AGCAAAGTTC CGAAAAGAAA TCATCACCAA AGGAAAATCC TATAGCAAAA 
GAAGAAATGG ATTCAAAACC AAAACTATCC AATAAATTTA TCACTTCAAT GAATGATGTG 
TCCACAGATG ATTCAAGCTC TGATGGTAAC GAAAATGACG ATGCAGACGA TGATGATGAT 
TTTACCTATA TGGCATTGAA ACAAACAATG AAGAGAGAAG GTTCAAAAAT TGAAAAAAAT 
AATGACAGCA AACTACCTGC AAATATAGTG GAACTTGATT TACATGAGTC ACCGGAGTCC 
GTGAAGATTG AATCTCCTGA ATCGATAAAG GAAATCACGT CATCTGAAAT GTCTTCAGAA 
ATGCCAAGTA GTTCGCTGCC TAAGAGATTA GTAGAGGATG TTGAGCCTTC AGAAATGCCA 
GAGAAGGGCG CATCTGTAGA ATCAGTCAGG AAGAAAAATT TTCAAGAACC ACTTGGTAAT 
GTCGAATCTC CGGATATGAC GCAGAAGGTC AAGTCTTTGG GTATGACAGG AAAGGCTGTA 
GGCCCAGAAT CAGATAGTAG GGTCGAATCT CCGGGCATGA CAGGACAGAT TAAATCTTTG 
AATATGGCAG GAAAAGTTGT AGGCCCAGAA GCAGATAGTA GGGTCGAATC TCCGGGCATG 
AAAGAGCAGA TTAAGTCTTT GGGTATGACA GGAAAAATTA CAGCTCAAGA ATCAATCAAG 
TCCCCGGAAG CGGCTAGGAA GTTGGCGTCA TCAGGAGAAG TTGACAAAAT TGAATCTCCA 
AGAATGGTAA GGGAAAGCGA GTCCTTGGAG GCAGTAGGCA ATACTATCCC CTCAAACATG 
ACAGTGAAAA TGGAATCCCC AAATTTAAAG GGAAATACTG TGTCTGAACC TCAAGAAATA 
AGGAGAGACA TTGCCTCCTC AGAGCCGATA GAGAATGTTG ACCCCCCAAA AGTACTAAAA 
AAGATTGTCT TTCCAAAGGC TGTTAATAGA ACTGGATCAC CAAAATCAGT CGAAAAGACT 
CCATCTTCAG CTACACTGAA AAAGAGCGGG CTCCCAGAAC CGAATAGCCA AATTGTTTCT 
CCTGAATTGG CAAAAAATTC ACCTCTAGCA CCGATAAAGA AAAATGTCGA GTTACGAGAA 
ACTAATAAAC CACATACTGA GACTATCACT TCTGTGGAAC CAACAAACAA GGATGCCAAT 
ACTTCTTGGA GAGACGCCGA CTTAAACCGT ACGATCAAAC GAGAGGAGGA GGACGAAGAT 
TTTGATAGAG TGAACCACAA TATCCAGATC ACTGGTGCAT ATACGAAAAC TGGAAAAATT 
GATTATCATA AAATACCTGT TGATCGTAAA GCAAAAAGCG AAGCAGAAGT GCATACTTCC 
GAGGAAGATA TTGATGAATC AAATAATGTT AATGGAAAAA GAGCTGATGC CCAAATACAC 
ATCACTGAAA GAAAGCATGC TTTCGTAAAT CCAACTGAAA ATTCACAGGT AAAAAAGACG 
AGCCACTCAC CATTTTTAAA CAGTAAACCG GTTCAATACG AGAACTCAGA GTCGAACGGC 
GGCATTAACA ACCACATAAA GATAAAAAAT ACTGGAGAAA CTACGGCACA TGACGAGAAA 
CATTATAGTG ATGATGATGA TTCTAGCTAT CAATTTGTTC CCATGAAACA TGAAGAACAA 
GAACAAGAAC AAAACAGAAG TGAGGAAGAG GAAAGTGAAG ATGACGATGA AGAGGAAGAA 
GACAGTGATT TTGATGTGGA TACATTTGAC ATTGAAAATC CGGATAATAC ACTATCAGAG 
TTACTATTGT ATTTAGAACA TCAAACAATG GACGTCATAT CCACGATTCA ATCGCTTTTG 
ACATCGATCA AGAAACCACA GGTGACGAAG GGTAATTTGA GGGGAGAATC GAATGCAATC 
AACCAAGTCA TAGGTCAAAT GGTGGACGCT ACTAGCATAT CAATGGAGCA AAGCAGAAAT 
GCCAATTTGA AGAAACACGG TGATTGGGTG GTGCAAAGTC TAAGAGACTG TTCGCGTAGA 
ATGACAATTT TGTGCCAATT AACTGGCGAT GGAATACTAG CGAAGGAAAA GAGCGATCAA 
GATTATGCTG ACAAAAACTT CAAACAGCGG TTGGCAGGGA TTGCGTTTGA TGTTGCCAAA 
TGTACAAAGG AGCTGGTAAA AACTGTAGAA GAGGCAAGTT TGAAGGACGA AATAAATTAT 
TTGAATTCGA AGTTGAAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XII.
Nature387(6632 Suppl)87-90(1997 May)
Johnston M,Hillier L,Riles L,Albermann K,Andr? B,Ansorge W,Benes V,Br?ckner M,Delius H,Dubois E,D?sterh?ft A,Entian KD,Floeth M,Goffeau A,Hebling U,Heumann K,Heuss-Neitzel D,Hilbert H,Hilger F,Kleine K,K?tter P,Louis EJ,Messenguy F,Mewes HW,Hoheisel JD

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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SPA2 cDNA ORF clone, Saccharomyces cerevisiae S288C

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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