ORF » Species Summary » Saccharomyces cerevisiae S288C » ISA1 cDNA ORF clone

ISA1 cDNA ORF clone, Saccharomyces cerevisiae S288C

The following ISA1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ISA1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OSi00305 NM_001181847.1
Latest version!		 Saccharomyces cerevisiae S288C Fe-binding Fe/S cluster assembly protein ISA1 (ISA1), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $174.30
$249.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    ISA1 ( NM_001181847.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_013073.1 Saccharomyces cerevisiae S288C Fe-binding Fe/S cluster assembly protein ISA1 (ISA1), partial mRNA.
    Price & Availability↑
    CloneID OSi00305
    Clone ID Related Accession (Same CDS sequence) NM_001181847.1
    Accession Version NM_001181847.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 753bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-31
    Organism Saccharomyces cerevisiae S288C
    Product Fe-binding Fe/S cluster assembly protein ISA1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001144). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    ATGATAAACA CAGGAAGAAG CAGGAATTCA GTACTGCTGG CACACAGATT CCTTTCAACT 
    GGAGGATTTT GGCGAGGCGG TACGAATGGC ACAATGTCTC GCACAATCAA CAACGTAAAT 
    CCTTTCAAAT TAAAATTCAT ACCGAAGACA GTGCCCGCAG CTGCAGACTC TGTCTCTCCA 
    GATAGTCAAC GTCCAGGTAA GAAGCCCTTC AAATTCATAG TTTCCAATCA GAGCAAGAGT 
    AGCAAGGCTT CTAAAAGCCC GAAGTGGTCG AGCTACGCAT TCCCTTCGCG TGAGACCATC 
    AAATCTCATG AGGAGGCCAT CAAGAAGCAG AATAAAGCTA TAGACGAGCA AATAGCTGCT 
    GCAGTATCCA AGAATGACTG CTCTTGCACA GAACCTCCCA AGAAAAGAAA GAGGAAATTG 
    AGACCAAGAA AGGCGCTGAT CACCCTGAGT CCGAAGGCAA TCAAGCATTT AAGGGCACTG 
    CTAGCTCAGC CGGAACCTAA ATTGATTAGA GTTAGCGCTA GAAACCGTGG ATGTTCAGGA 
    CTAACGTACG ATCTACAATA TATCACCGAG CCGGGGAAAT TCGATGAGGT AGTAGAACAA 
    GATGGCGTTA AAATTGTCAT CGATTCAAAG GCGTTATTCA GCATCATTGG AAGTGAAATG 
    GACTGGATCG ACGACAAGTT GGCCTCTAAG TTTGTCTTCA AGAATCCAAA CTCCAAGGGC 
    ACATGCGGTT GTGGCGAGAG TTTCATGGTT TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_013073.1
    CDS1..753
    Translation

    Target ORF information:

    RefSeq Version NM_001181847.1
    Organism Saccharomyces cerevisiae S288C
    Definition Saccharomyces cerevisiae S288C Fe-binding Fe/S cluster assembly protein ISA1 (ISA1), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001181847.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    ATGATAAACA CAGGAAGAAG CAGGAATTCA GTACTGCTGG CACACAGATT CCTTTCAACT 
    GGAGGATTTT GGCGAGGCGG TACGAATGGC ACAATGTCTC GCACAATCAA CAACGTAAAT 
    CCTTTCAAAT TAAAATTCAT ACCGAAGACA GTGCCCGCAG CTGCAGACTC TGTCTCTCCA 
    GATAGTCAAC GTCCAGGTAA GAAGCCCTTC AAATTCATAG TTTCCAATCA GAGCAAGAGT 
    AGCAAGGCTT CTAAAAGCCC GAAGTGGTCG AGCTACGCAT TCCCTTCGCG TGAGACCATC 
    AAATCTCATG AGGAGGCCAT CAAGAAGCAG AATAAAGCTA TAGACGAGCA AATAGCTGCT 
    GCAGTATCCA AGAATGACTG CTCTTGCACA GAACCTCCCA AGAAAAGAAA GAGGAAATTG 
    AGACCAAGAA AGGCGCTGAT CACCCTGAGT CCGAAGGCAA TCAAGCATTT AAGGGCACTG 
    CTAGCTCAGC CGGAACCTAA ATTGATTAGA GTTAGCGCTA GAAACCGTGG ATGTTCAGGA 
    CTAACGTACG ATCTACAATA TATCACCGAG CCGGGGAAAT TCGATGAGGT AGTAGAACAA 
    GATGGCGTTA AAATTGTCAT CGATTCAAAG GCGTTATTCA GCATCATTGG AAGTGAAATG 
    GACTGGATCG ACGACAAGTT GGCCTCTAAG TTTGTCTTCA AGAATCCAAA CTCCAAGGGC 
    ACATGCGGTT GTGGCGAGAG TTTCATGGTT TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The nucleotide sequence of Saccharomyces cerevisiae chromosome XII.
    Nature387(6632 Suppl)87-90(1997 May)
    Johnston M,Hillier L,Riles L,Albermann K,Andr? B,Ansorge W,Benes V,Br?ckner M,Delius H,Dubois E,D?sterh?ft A,Entian KD,Floeth M,Goffeau A,Hebling U,Heumann K,Heuss-Neitzel D,Hilbert H,Hilger F,Kleine K,K?tter P,Louis EJ,Messenguy F,Mewes HW,Hoheisel JD


    Life with 6000 genes.
    Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
    Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG


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