GCN20 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	GCN20
Entrez Gene ID	850561
Full Name	putative AAA family ATPase GCN20
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001179974.1	NP_116664.1	putative AAA family ATPase GCN20

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Abcf3	NP_038880.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012005	XP_320530.4
Schizosaccharomyces pombe (fission yeast)	SPBC29A3.09c	NP_595837.1
Canis lupus familiaris (dog)	ABCF3	XP_849485.1
Bos taurus (cattle)	ABCF3	NP_001076926.1
Arabidopsis thaliana (thale cress)	GCN3	NP_176636.1
Homo sapiens (human)	ABCF3	NP_060828.2
Gallus gallus (chicken)	ABCF3	XP_004943454.1
Danio rerio (zebrafish)	LOC100331227	XP_002667519.2
Pan troglodytes (chimpanzee)	ABCF3	XP_516910.2
	ABCF3	XP_001100584.2
Drosophila melanogaster (fruit fly)	CG9330	NP_649129.1
Caenorhabditis elegans (roundworm)	abcf-3	NP_498339.1
Neurospora crassa	NCU04051	XP_957666.1
Xenopus tropicalis (tropical clawed frog)	LOC100145696	NP_001120542.1
Rattus norvegicus (Norway rat)	Abcf3	NP_001011896.1
Saccharomyces cerevisiae (baker's yeast)	GCN20	NP_116664.1
Kluyveromyces lactis	KLLA0A10857g	XP_451473.1
Eremothecium gossypii	AGOS_AEL032W	NP_984829.1
Magnaporthe oryzae (rice blast fungus)	MGG_11547	XP_003718434.1

Gene Ontology
Bibliography

Related articles in PubMed

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

Analysis of the nucleotide sequence of chromosome VI from Saccharomyces cerevisiae.
Murakami Y, Naitou M, Hagiwara H, Shibata T, Ozawa M, Sasanuma S, Sasanuma M, Tsuchiya Y, Soeda E, Yokoyama K
Nature genetics10(3)261-8(1995 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Snf1 functions upstream of Gcn20 to regulate control of GCN4 translation in S. cerevisiae
Title: A chemical genomics study identifies Snf1 as a repressor of GCN4 translation.

The following GCN20 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GCN20 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi00245	NM_001179974.1 Latest version!	Saccharomyces cerevisiae S288C putative AAA family ATPase GCN20 (GCN20), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GCN20 ( NM_001179974.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_116664.1 Saccharomyces cerevisiae S288C putative AAA family ATPase GCN20 (GCN20), partial mRNA.

Price & Availability↑

CloneID	OSi00245
Clone ID Related Accession (Same CDS sequence)	NM_001179974.1
Accession Version	NM_001179974.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2259bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	putative AAA family ATPase GCN20
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001138). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGGCAAGCA TCGGTTCGCA AGTGAGAAAA GCTGCTTCTA GTATTGACCC TATCGTCACG 
GATTACGCAG TGGGCTACTT TAACCACTTG TCCGGAATAA CTTTTGATGC TGTTCAAAGT 
AAGCAGGTAG ATTTGTCCAC TGAAGTGCAA TTTGTGTCCG ATTTATTGAT TGATGCGGGT 
GCGTCAAAGG CTAAAGTTAA AGAACTATCG GAAAGTATTT TGAAGCAATT GACTACTCAA 
CTAAAGGAGA ACGAAGCCAA ATTGGAATTG ACCGGTGATA CGTCCAAGAG ATTACTTGAT 
ATTAATGTCT TAAAGAGTCA TAACAGTAAA TCCGATATCA ACGTCTCATT AAGCATGCTG 
GGTGTGAACG GTGACATCGA ACATACTGGT AGAAAGATGG AAACAAGAGT TGATTTGAAA 
AAACTGGCCA AGGCTGAACA AAAGATCGCA AAGAAAGTCG CCAAGAGAAA TAACAAATTT 
GTTAAATACG AGGCTTCTAA ATTGATCAAT GACCAAAAGG AGGAGGATTA CGATTCTTTC 
TTTTTGCAAA TCAACCCTTT AGAATTCGGT TCATCCGCTG GTAAATCCAA GGATATCCAT 
ATTGACACTT TCGACTTGTA CGTTGGTGAC GGTCAAAGAA TTTTGTCCAA CGCCCAATTG 
ACTCTAAGTT TTGGTCACAG ATATGGTCTT GTGGGCCAAA ATGGTATTGG TAAATCTACT 
TTGTTAAGGG CTCTATCTAG AAGAGAGCTG AACGTCCCCA AACATGTTTC GATTTTACAC 
GTGGAACAAG AGTTAAGAGG TGATGATACA AAGGCTTTAC AAAGTGTGCT GGATGCAGAC 
GTTTGGAGAA AACAACTATT AAGTGAAGAA GCCAAGATCA ATGAAAGATT AAAGGAAATG 
GATGTATTAA GACAGGAATT CGAAGAAGAC AGTTTAGAAG TTAAAAAATT GGACAATGAA 
AGAGAAGACT TGGATAACCA TTTGATACAG ATTTCTGACA AATTAGTCGA TATGGAATCT 
GACAAGGCTG AAGCTAGGGC AGCATCAATC TTATATGGTT TGGGGTTCAG TACGGAGGCA 
CAGCAACAAC CCACTAATTC CTTTTCCGGT GGTTGGAGAA TGAGATTGTC CTTGGCAAGA 
GCCTTATTCT GTCAACCAGA TCTTTTGTTG TTAGATGAAC CTTCCAATAT GTTGGATGTG 
CCATCCATCG CTTATTTAGC AGAGTATTTG AAAACATATC CAAATACAGT TTTGACAGTT 
TCTCACGACC GTGCATTCTT GAATGAAGTG GCTACAGATA TCATTTATCA ACACAACGAA 
AGACTAGACT ATTACAGAGG CCAAGATTTC GATACCTTTT ACACCACAAA GGAGGAACGT 
AGAAAGAATG CTCAACGTGA GTATGATAAC CAAATGGTTT ACAGAAAGCA CTTGCAAGAG 
TTTATTGACA AATACAGATA CAATGCTGCC AAATCACAGG AAGCTCAATC AAGAATTAAG 
AAATTGGAAA AATTGCCCGT TTTGGAGCCA CCTGAACAAG ACAAAACCAT TGATTTCAAA 
TTCCCTGAAT GTGATAAATT GTCTCCACCA ATTATCCAAT TGCAAGACGT TTCCTTTGGT 
TATGATGAAA ACAACCTATT ATTGAAAGAT GTTAACCTGG ACGTTCAAAT GGATTCCAGA 
ATTGCCCTTG TAGGTGCCAA TGGTTGTGGT AAGACTACAC TGTTGAAGAT TATGATGGAG 
CAGTTAAGAC CACTAAAAGG CTTTGTATCA AGAAACCCAA GATTACGTAT AGGCTACTTC 
ACTCAACATC ATGTGGATTC TATGGATTTG ACCACGTCTG CAGTGGACTG GATGTCCAAA 
TCCTTCCCAG GTAAAACTGA TGAAGAGTAT AGACGTCATC TAGGTTCATT TGGTATCACT 
GGTACCCTGG GTCTACAAAA GATGCAATTA TTATCCGGTG GTCAAAAATC TCGTGTAGCA 
TTCGCTGCAT TGTGTTTAAA TAATCCACAC ATTTTGGTTC TGGATGAACC TTCTAACCAT 
TTGGATACCA CTGGTCTAGA CGCTTTGGTA GAAGCCTTGA AAAATTTCAA CGGTGGTGTC 
TTAATGGTTT CCCATGATAT CTCTGTTATT GACTCTGTTT GTAAAGAGAT TTGGGTTTCA 
GAGCAAGGTA CTGTCAAGAG GTTCGAAGGT ACAATTTACG ACTATAGAGA TTACATCTTG 
CAGTCTGCTG ATGCTGCAGG TGTGGTTAAA AAGCATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_116664.1
CDS	1..2259
Translation	MASIGSQVRKAASSIDPIVTDYAVGYFNHLSGITFDAVQSKQVDLSTEVQFVSDLLIDAGASKAKVKELSESILKQLTTQLKENEAKLELTGDTSKRLLDINVLKSHNSKSDINVSLSMLGVNGDIEHTGRKMETRVDLKKLAKAEQKIAKKVAKRNNKFVKYEASKLINDQKEEDYDSFFLQINPLEFGSSAGKSKDIHIDTFDLYVGDGQRILSNAQLTLSFGHRYGLVGQNGIGKSTLLRALSRRELNVPKHVSILHVEQELRGDDTKALQSVLDADVWRKQLLSEEAKINERLKEMDVLRQEFEEDSLEVKKLDNEREDLDNHLIQISDKLVDMESDKAEARAASILYGLGFSTEAQQQPTNSFSGGWRMRLSLARALFCQPDLLLLDEPSNMLDVPSIAYLAEYLKTYPNTVLTVSHDRAFLNEVATDIIYQHNERLDYYRGQDFDTFYTTKEERRKNAQREYDNQMVYRKHLQEFIDKYRYNAAKSQEAQSRIKKLEKLPVLEPPEQDKTIDFKFPECDKLSPPIIQLQDVSFGYDENNLLLKDVNLDVQMDSRIALVGANGCGKTTLLKIMMEQLRPLKGFVSRNPRLRIGYFTQHHVDSMDLTTSAVDWMSKSFPGKTDEEYRRHLGSFGITGTLGLQKMQLLSGGQKSRVAFAALCLNNPHILVLDEPSNHLDTTGLDALVEALKNFNGGVLMVSHDISVIDSVCKEIWVSEQGTVKRFEGTIYDYRDYILQSADAAGVVKKH

Target ORF information:

RefSeq Version	NM_001179974.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C putative AAA family ATPase GCN20 (GCN20), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001179974.1

ORF Insert Sequence:

ATGGCAAGCA TCGGTTCGCA AGTGAGAAAA GCTGCTTCTA GTATTGACCC TATCGTCACG 
GATTACGCAG TGGGCTACTT TAACCACTTG TCCGGAATAA CTTTTGATGC TGTTCAAAGT 
AAGCAGGTAG ATTTGTCCAC TGAAGTGCAA TTTGTGTCCG ATTTATTGAT TGATGCGGGT 
GCGTCAAAGG CTAAAGTTAA AGAACTATCG GAAAGTATTT TGAAGCAATT GACTACTCAA 
CTAAAGGAGA ACGAAGCCAA ATTGGAATTG ACCGGTGATA CGTCCAAGAG ATTACTTGAT 
ATTAATGTCT TAAAGAGTCA TAACAGTAAA TCCGATATCA ACGTCTCATT AAGCATGCTG 
GGTGTGAACG GTGACATCGA ACATACTGGT AGAAAGATGG AAACAAGAGT TGATTTGAAA 
AAACTGGCCA AGGCTGAACA AAAGATCGCA AAGAAAGTCG CCAAGAGAAA TAACAAATTT 
GTTAAATACG AGGCTTCTAA ATTGATCAAT GACCAAAAGG AGGAGGATTA CGATTCTTTC 
TTTTTGCAAA TCAACCCTTT AGAATTCGGT TCATCCGCTG GTAAATCCAA GGATATCCAT 
ATTGACACTT TCGACTTGTA CGTTGGTGAC GGTCAAAGAA TTTTGTCCAA CGCCCAATTG 
ACTCTAAGTT TTGGTCACAG ATATGGTCTT GTGGGCCAAA ATGGTATTGG TAAATCTACT 
TTGTTAAGGG CTCTATCTAG AAGAGAGCTG AACGTCCCCA AACATGTTTC GATTTTACAC 
GTGGAACAAG AGTTAAGAGG TGATGATACA AAGGCTTTAC AAAGTGTGCT GGATGCAGAC 
GTTTGGAGAA AACAACTATT AAGTGAAGAA GCCAAGATCA ATGAAAGATT AAAGGAAATG 
GATGTATTAA GACAGGAATT CGAAGAAGAC AGTTTAGAAG TTAAAAAATT GGACAATGAA 
AGAGAAGACT TGGATAACCA TTTGATACAG ATTTCTGACA AATTAGTCGA TATGGAATCT 
GACAAGGCTG AAGCTAGGGC AGCATCAATC TTATATGGTT TGGGGTTCAG TACGGAGGCA 
CAGCAACAAC CCACTAATTC CTTTTCCGGT GGTTGGAGAA TGAGATTGTC CTTGGCAAGA 
GCCTTATTCT GTCAACCAGA TCTTTTGTTG TTAGATGAAC CTTCCAATAT GTTGGATGTG 
CCATCCATCG CTTATTTAGC AGAGTATTTG AAAACATATC CAAATACAGT TTTGACAGTT 
TCTCACGACC GTGCATTCTT GAATGAAGTG GCTACAGATA TCATTTATCA ACACAACGAA 
AGACTAGACT ATTACAGAGG CCAAGATTTC GATACCTTTT ACACCACAAA GGAGGAACGT 
AGAAAGAATG CTCAACGTGA GTATGATAAC CAAATGGTTT ACAGAAAGCA CTTGCAAGAG 
TTTATTGACA AATACAGATA CAATGCTGCC AAATCACAGG AAGCTCAATC AAGAATTAAG 
AAATTGGAAA AATTGCCCGT TTTGGAGCCA CCTGAACAAG ACAAAACCAT TGATTTCAAA 
TTCCCTGAAT GTGATAAATT GTCTCCACCA ATTATCCAAT TGCAAGACGT TTCCTTTGGT 
TATGATGAAA ACAACCTATT ATTGAAAGAT GTTAACCTGG ACGTTCAAAT GGATTCCAGA 
ATTGCCCTTG TAGGTGCCAA TGGTTGTGGT AAGACTACAC TGTTGAAGAT TATGATGGAG 
CAGTTAAGAC CACTAAAAGG CTTTGTATCA AGAAACCCAA GATTACGTAT AGGCTACTTC 
ACTCAACATC ATGTGGATTC TATGGATTTG ACCACGTCTG CAGTGGACTG GATGTCCAAA 
TCCTTCCCAG GTAAAACTGA TGAAGAGTAT AGACGTCATC TAGGTTCATT TGGTATCACT 
GGTACCCTGG GTCTACAAAA GATGCAATTA TTATCCGGTG GTCAAAAATC TCGTGTAGCA 
TTCGCTGCAT TGTGTTTAAA TAATCCACAC ATTTTGGTTC TGGATGAACC TTCTAACCAT 
TTGGATACCA CTGGTCTAGA CGCTTTGGTA GAAGCCTTGA AAAATTTCAA CGGTGGTGTC 
TTAATGGTTT CCCATGATAT CTCTGTTATT GACTCTGTTT GTAAAGAGAT TTGGGTTTCA 
GAGCAAGGTA CTGTCAAGAG GTTCGAAGGT ACAATTTACG ACTATAGAGA TTACATCTTG 
CAGTCTGCTG ATGCTGCAGG TGTGGTTAAA AAGCATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

Analysis of the nucleotide sequence of chromosome VI from Saccharomyces cerevisiae.
Nature genetics10(3)261-8(1995 Jul)
Murakami Y,Naitou M,Hagiwara H,Shibata T,Ozawa M,Sasanuma S,Sasanuma M,Tsuchiya Y,Soeda E,Yokoyama K

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GCN20 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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