LOC1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC1
Entrez Gene ID	850551
Full Name	Loc1p
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001179966.1	NP_116656.1	Loc1p

Pathways from BioSystems

Homologies

Saccharomyces cerevisiae (baker's yeast)	LOC1	NP_116656.1
Eremothecium gossypii	AGOS_AGR001W	NP_986666.1
Kluyveromyces lactis	KLLA0E14257g	XP_454593.1

Gene Ontology
Bibliography

Related articles in PubMed

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

Analysis of the nucleotide sequence of chromosome VI from Saccharomyces cerevisiae.
Murakami Y, Naitou M, Hagiwara H, Shibata T, Ozawa M, Sasanuma S, Sasanuma M, Tsuchiya Y, Soeda E, Yokoyama K
Nature genetics10(3)261-8(1995 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Puf6 and Loc1 facilitate Rpl43 loading and are sequentially released from 60S after incorporation of Rpl43 into ribosomes in yeast.
Title: The Roles of Puf6 and Loc1 in 60S Biogenesis Are Interdependent, and Both Are Required for Efficient Accommodation of Rpl43.

She2p interacts with both Loc1p and Puf6p and excluding She2p from the nucleus decreases this interaction.
Title: Nuclear shuttling of She2p couples ASH1 mRNA localization to its translational repression by recruiting Loc1p and Puf6p.

has a role in rRNA processing and nuclear export of 60S subunits
Title: Loc1p is required for efficient assembly and nuclear export of the 60S ribosomal subunit.

The following LOC1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi00237	NM_001179966.1 Latest version!	Saccharomyces cerevisiae S288C Loc1p (LOC1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC1 ( NM_001179966.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_116656.1 Saccharomyces cerevisiae S288C Loc1p (LOC1), partial mRNA.

Price & Availability↑

CloneID	OSi00237
Clone ID Related Accession (Same CDS sequence)	NM_001179966.1
Accession Version	NM_001179966.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 615bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	Loc1p
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001138). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGGCACCAA AGAAACCTTC TAAGAGACAA AATCTGAGAA GAGAAGTCGC ACCAGAGGTG 
TTTCAAGATT CACAAGCTAG GAATCAACTA GCGAATGTTC CTCATCTTAC CGAAAAATCT 
GCCCAGCGTA AGCCTTCTAA AACCAAGGTT AAAAAAGAAC AGTCTTTGGC TAGACTTTAT 
GGTGCGAAGA AGGACAAGAA GGGGAAATAT TCTGAGAAAG ACTTGAATAT TCCAACACTC 
AATAGAGCTA TCGTTCCGGG TGTTAAAATA AGGAGGGGAA AGAAAGGTAA GAAATTCATT 
GCTGATAACG ACACTCTGAC TTTAAACCGT TTAATAACAA CTATTGGTGA CAAGTACGAC 
GATATAGCTG AGAGTAAGCT TGAAAAGGCT AGAAGATTAG AAGAGATACG AGAATTGAAA 
AGAAAGGAAA TTGAAAGAAA GGAAGCGCTT AAACAAGATA AACTAGAAGA AAAAAAAGAC 
GAGATTAAAA AGAAGTCTTC TGTCGCAAGG ACTATACGTA GAAAGAATAA ACGTGATATG 
TTGAAAAGTG AAGCAAAAGC TAGTGAAAGT AAAACTGAAG GAAGGAAGGT AAAAAAAGTC 
TCATTTGCTC AATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_116656.1
CDS	1..615
Translation	MAPKKPSKRQNLRREVAPEVFQDSQARNQLANVPHLTEKSAQRKPSKTKVKKEQSLARLYGAKKDKKGKYSEKDLNIPTLNRAIVPGVKIRRGKKGKKFIADNDTLTLNRLITTIGDKYDDIAESKLEKARRLEEIRELKRKEIERKEALKQDKLEEKKDEIKKKSSVARTIRRKNKRDMLKSEAKASESKTEGRKVKKVSFAQ

Target ORF information:

RefSeq Version	NM_001179966.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C Loc1p (LOC1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001179966.1

ORF Insert Sequence:

ATGGCACCAA AGAAACCTTC TAAGAGACAA AATCTGAGAA GAGAAGTCGC ACCAGAGGTG 
TTTCAAGATT CACAAGCTAG GAATCAACTA GCGAATGTTC CTCATCTTAC CGAAAAATCT 
GCCCAGCGTA AGCCTTCTAA AACCAAGGTT AAAAAAGAAC AGTCTTTGGC TAGACTTTAT 
GGTGCGAAGA AGGACAAGAA GGGGAAATAT TCTGAGAAAG ACTTGAATAT TCCAACACTC 
AATAGAGCTA TCGTTCCGGG TGTTAAAATA AGGAGGGGAA AGAAAGGTAA GAAATTCATT 
GCTGATAACG ACACTCTGAC TTTAAACCGT TTAATAACAA CTATTGGTGA CAAGTACGAC 
GATATAGCTG AGAGTAAGCT TGAAAAGGCT AGAAGATTAG AAGAGATACG AGAATTGAAA 
AGAAAGGAAA TTGAAAGAAA GGAAGCGCTT AAACAAGATA AACTAGAAGA AAAAAAAGAC 
GAGATTAAAA AGAAGTCTTC TGTCGCAAGG ACTATACGTA GAAAGAATAA ACGTGATATG 
TTGAAAAGTG AAGCAAAAGC TAGTGAAAGT AAAACTGAAG GAAGGAAGGT AAAAAAAGTC 
TCATTTGCTC AATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

Analysis of the nucleotide sequence of chromosome VI from Saccharomyces cerevisiae.
Nature genetics10(3)261-8(1995 Jul)
Murakami Y,Naitou M,Hagiwara H,Shibata T,Ozawa M,Sasanuma S,Sasanuma M,Tsuchiya Y,Soeda E,Yokoyama K

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LOC1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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