BLM10 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	BLM10
Entrez Gene ID	850541
Full Name	Blm10p
Synonyms	YFL006W
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001179959.1	NP_116648.2	Blm10p

Pathways from BioSystems

Homologies

Eremothecium gossypii	AGOS_AGL022W	NP_986644.2
Magnaporthe oryzae (rice blast fungus)	MGG_04342	XP_003719463.1
Saccharomyces cerevisiae (baker's yeast)	BLM10	NP_116648.2
Kluyveromyces lactis	KLLA0E12057g	XP_454493.1
Neurospora crassa	NCU05620	XP_960116.2

Gene Ontology
Bibliography

Related articles in PubMed

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

Analysis of the nucleotide sequence of chromosome VI from Saccharomyces cerevisiae.
Murakami Y, Naitou M, Hagiwara H, Shibata T, Ozawa M, Sasanuma S, Sasanuma M, Tsuchiya Y, Soeda E, Yokoyama K
Nature genetics10(3)261-8(1995 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

data are consistent with a model in which Blm10 proteasome-mediated turnover of Dnm1 is required for the maintenance of mitochondrial function
Title: Proteasomes associated with the Blm10 activator protein antagonize mitochondrial fission through degradation of the fission protein Dnm1.

Blm10 represents the first proteolytic core particle-dedicated nuclear import receptor in yeast.
Title: Blm10 facilitates nuclear import of proteasome core particles.

Data propose that the cellular function of Blm10 is based on central core of the proteasome association and activation to promote the degradation of proteasome substrates.
Title: Blm10 protein promotes proteasomal substrate turnover by an active gating mechanism.

Blm10 functions in a proteasome-related process and it is required for the maintenance of mitochondrial function.
Title: Structure of a Blm10 complex reveals common mechanisms for proteasome binding and gate opening.

Blm10 distinguishes between gate conformations and regulates the activation of core particles.
Title: Blm10 binds to pre-activated proteasome core particles with open gate conformation.

The following BLM10 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the BLM10 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi00229	NM_001179959.1 Latest version!	Saccharomyces cerevisiae S288C Blm10p (BLM10), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

BLM10 ( NM_001179959.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_116648.2 Saccharomyces cerevisiae S288C Blm10p (BLM10), partial mRNA.

Price & Availability↑

CloneID	OSi00229
Clone ID Related Accession (Same CDS sequence)	NM_001179959.1
Accession Version	NM_001179959.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6432bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	Blm10p
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001138). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGACCGCTA ACAATGACGA TGATATCAAA TCACCCATTC CCATTACTAA CAAGACCTTA 
TCCCAATTGA AGCGCTTTGA GAGAAGTCCA GGAAGGCCCA GTTCTTCTCA GGGCGAGATA 
AAACGTAAAA AGTCTAGGCT ATATGCCGCA GACGGAAGAC CACATTCTCC GCTAAGAGCA 
AGGTCTGCTA CCCCAACGCT ACAGGACCAA AAACTGTTCA ATGGCATGGA TTCCACTTCC 
CTTTTGAATG AAAGGCTACA GCATTATACG CTGGATTATG TTAGTGACAG GGCCCAGCAT 
ATGAAGAATA TATATGACCC ATCATCTCGC TGGTTCAGCA GATCCGTGAG GCCTGAATTT 
CCTATTGAAG AGTTCTTACC ATATAAGACT GAAAGTCATG AAGATCAAGC GAAATACTTG 
TGCCATGTCT TAGTTAATCT TTATATTGCG ATCAGCTCAT TAGATATACA AGGTTTGATT 
TCTATTTCCA GTAAAGATCT GGCTGATTTA AAGAAAGAAG TGGATGATTT AGCTCTTAAA 
ACCGATCTTT TCAGGTTATC TAACAACACA GCGGAGAATG ACTTACTTGG TAACGATATT 
GCTGATTATG ACGATGCGGA AGGCCTGGAG GACGAATTGG ATGAATACTT CGACTTAGCA 
GGCCCCGACT TTAATGCTAC CGGAAAAATC ACAGCTAAAT CAGCTACTAT TGTGAATGTA 
AACCATTGGA CTAATGAGCT CAAAAATTGT CTACATTTTG ATTTTCCAGT AGCTTTAAGA 
AAGTCGCTAG CGACAGTTTA TTATTATTTG TCTCTTGTTC AGGGCCAAAA GGTGTATAGA 
CAAATGCATG TCGATATGTT CGAAAGATTA GTAAGCCTTG ACGATGATAG GACAAATTTC 
ACTGAACTGT TGCAAAAACA AGGTCTTTTG CTAGATCATC AAATCATGCT CAATTTCCTG 
TGCGAATTTC TACCTTATCC AGATCCCGAC TATGCTCGTT ATGAATTATC ATCAAAGGAA 
GATTTACAGT TATTTAGATT ACTTTTGAAG CATGCACATA ATGCAAAACC ATTTTTCGAT 
AAGTCAAAGG AAAGTTTGTT AGTTGATACG ATGAATTTTC TGTTGTCTAG TCTTGCACCA 
TCTACTATGA TGGCTGTCAT GCCTATCGTT ACATCCGTTG TGCCCTATCA TTATCATATC 
CATTCTAAGA TCATCGATTA TTTCCCGTTT TGCTATAGCA TCTGGAGCTC AGTCAGCGCA 
AACGTGGCCA TCGACACTCA CATGTATGAT TTTGTTGGGT CAATTTCCAA AGACGTTCAT 
AATAAGATTT TGAGTAGCGA GCATGAAAAG GATGTAGTTG GAGTGGAGTT TGGAGAATTC 
GGGATTTTTA CTGATGATCA AATGACTTTT ATGTTCAATA GGTTACAAGG CCATCTTAGA 
ACAGACGGTC AAATACATTC GTATTCCCGC ACAGTGAAGC CTTTTGTTTA TGCTATAAAC 
GGATCAAAAA AAGATAGGTT TTTTGAAAAA CTTGTAAGTT TAGCCAAAGC AATCGAAACA 
TTTATCCATC CCTCTAATAA TGGGTTTTGG ACCAAGCCAA ATGCTAAATT CGTTCATGCA 
TTTATAAAGT CTTACCACGG AAGGGTCAAA TATGAAGAAG ATATTTGTGC TAGGGGCGTC 
ACAAATGGGA TATGTTTAAC TTCTTTTTGT CACGAAGAGA TAGTTGAAAT ATTCTTAAAT 
ATTATCAGTC TGGGTTCACA GAATAAAAAT CCTGATATTG CGAACTATTA CATCTCTTGT 
TTCGCATATC TGTTAGAGCT GGATCCTTCA AATGCATATT TAATTTATGA CAAAATACTG 
ATAGATTTGT ACGATACGCT GGCTGACCAA TTTATCAATT CGAGACACAG AATCATTTCC 
TCTTTGAAAC AATTTACAAG AGTAATTCGG TTTATTGTGA TGGATAAGCT ATATCGCGTG 
CACATAACAA ACGTCCTTTC GATGCTGGTC TCCAAACTTG ATATGAACGA TACTAATTTG 
ACAAGCAACC TCATCAACGG TATTGTATCT ATAGCCGCTT TCATTCCTAT CCAAGACCTC 
ACTGGGGAGG ACGATTATAT ATCGTTTGAA TCGGATACTC TTCCTTTAGT TCAACAACAT 
TTTTATCATA TTAAGTGTGG CGAAAGTTCA AAGACCTTCC GAGTTGATGA TGAACTGTTA 
AATAACGCTT TCAAAGCTTC CACTACAGTT TTTCAAAGTA TGCTAAAAGT ATACGTAGAA 
AAAATTTTCC AATTGGTTGA TGTAGACTTA GAGGACTCTT TGGTTACTAA AATAAACCAA 
ACAACTATGA TTTTACAAGA GTCTATGGAC GATAAAATAT TTAATTATTT TGCTTCTTTA 
TTACAGAGAA ACTTCTGGAG TAATGACTCC TTCAAGGAAA AGGATCCAAA CTATGAATTA 
GTTACTATCC CACTAGCGGC TTTAGTAAGA AGGAATAATG GTTTGAGTAA AGAGTTGGTC 
AGAACTCTTT TATTCCATAT CAAAGAACAA ATCAAAAGAG GCGCCGGGTC TGTAAGAAGT 
ACTTCAGAGA TTCAACAGAG GGATGTTAAG TTAGTTTTAT ACTTAACTGC ACTAAATGAT 
GTCTTAAGGC AATGTCATGA ATCTCTATTG GAGTATAGCG ATGAGTTGAT AACATTCATG 
AAATATTTAT ACGACAACGT CACTAACCCG CCATTGGATG TTATCACATC TATTGTTATT 
CACAGTGCTT TAGCAACTCT ATGTACAACC GAAATAACTG ATTGTCGTCT ATTCCCAGAG 
GACTCTAAGA TTCCGGAAAA AGACAGATGG GGAGGACTAC AGTTCGATCC TCGAAGATTT 
GATAAACAGC ATTTGAGTTT TCAGTGGCAC GTACCTTCTA GTGACGAGAT AACTTTATCC 
ATAAGCATTC TAGAAAGCCT TTCCGAATAC TGTATTAACA ACGTAGAAGA ACTGATGAAA 
GCTCCAAGAC ATGATTCCGA ATATGGCGAT ATGATACAAA AATATGTTTT AGTTATGACA 
CATACGCTTT CCGGGTCAAG TTTACTTTTT GATCCAGATT TTAACAAATA TAGGACGCAA 
TCAAACTTAT CATACAGAGA GAAACTGATT TTATTAAAGA ATATACGCGA AAATAATTGT 
GACCCTCAAG AACTGGATAT TGATATTGAA CAAATTCGTT CTGGCAAGGA TGATGAAGAC 
TATATTGAGA GTAAGGACAT TGAAGCAGGG CTGAATGCAG GAGTTTCCGA TGTTGTGCAG 
TTAAGAGATG AGTTTCCGGA TGAATTAATT GTTGATGAAG AAGTAGTGTC TGAGATGCCA 
TCTGGTGTAA ATACCCCTAT CGCGGGGACG CATGGCACGG ACAATTCAGC TATGAGTTCG 
GATCTAGCTT TCAGGGATTT AGATATTTAC ACCTGTAATT ATTACTTCGG AAATACCACT 
GAGGAGAAGT TACAAAACCC ACAATATTTA CAAGTCCACA GAGTTAGAGC GCGCATTGGA 
CATTTCTTTC ACAAACTCTA TGTTTTTTTA TCTACAAACT TCGAAAACAA CACCAACATG 
TTCCAAATTC TATTGCACGG ATTGAAAGTT TGGTTCACAG ATCTGGGACA AGAAACGGTC 
TTCAATGAAG ACCCAAATGC CTTCATTGAC GTTGATTTCC TAGAAAACGT TCAATCTCTC 
TCACACGTAA ATGAGCCCTT CACGAGAACC AATTTTGCAA TCAGAGCAAA CAGTTTGCAC 
CAGAGTAGAG TTCTATTACA TTCAACAAAT AGAAAAGCTT CCAAGCTGGA AAACCTATTG 
TTAGTTGACA TCATACAGTT AGCGACATCC CTTTATCCTG ATATTTATAA ACCAGCACAG 
GGAACTTTGG TACACTGTAT GAAACAATTA GTTGGGTCAT ATGGCGTAGT CATCAATAAA 
ATTATTCCAT CATTAGAGAA AGCGATTAAG GATCATGATT ATATGAAAAT CCAAGTTATT 
TTAAATGTTT TGTTAATTAA GAAAATCCAT AGGAAGCTTA TGACGGATTA TAAAGACATC 
GGCAGATTGA TATTTCTGCT TATTGAATGT TGTCGTGTGA ATGAATTAGA GATTGGTATG 
TATGCAGATA AAATCTTAAC TGACATAGTG ATTGGGATCA AGATTCCTTC TAGTGTATGT 
GTCATTTCCG ATCAAGCTTT CTTACCTTTA GCACCTCCTG ATGGTACTAT TAATTTGCAA 
GTTGAAGCGG TAAAGCTTGC CAAAAAGAAA AAGCGTGAGT ACTACCTCTC TCTGTTAGTG 
GATTTGCAGG ACAAACTTTT AGACAAATTA GATAATGAAA AAGATATGGG GTGGAAGATA 
AGAATGTTCA TTTTACGTTT TGTTACACAA ATCCAATCGA ACCTCGAAAG CAAACCCGAT 
AAAAGAGCAG TATTTTCAAT AATCTCCCAA ATCTCCACAA AACATCCCGA AATCATACAT 
TTGGTTGTAA AGTCATTGTT GTCGACGTGC AACAAGATAA TATCTCTCTC TGACTATGAA 
TATGACATCA CCAGGGCCTA TAAGAATGAA TTCAATCCAT CATTTGTGGA AATACTGGAC 
ACTTCGACCA CAAGCTTCCC TAAAACGTTT ACTGAAGAAA TGAATAACTT TGATAACCCC 
AAGTATTTTA TCGATTTGAG GGCGTATGTA GGGTGGCTAT GTTGGGGAAG GCTTATGTAC 
GTCATGTCGC CGAAAGCTTT AAAGCTCAAT TTACGTGAGA ATGAACTGGA AGTCCTCAAG 
ACAGCTGGTC ATCTATTGAC AAGAGAATTC CTGAGAGATG TTACAATGAA TTTAGTCCAA 
GATAATGAAA CTAGGGGTGT TTTTAGTAGT GGTAACGTGT CATTTTTCTC TTTAGTAATC 
CTTTTGATAT CATCTGGTTT CTGCGAACTG AATATGTCGG ATCTCTTTGA GCTATGTGAA 
TCCTACTATA ACAAAGACGA TAAGGCTTCG ATGATCATGT CTGTCGAGAT AGTGGCTGGC 
TTAGTTTGCG GGAGTAAGTT TATGTCAGTC TCTGACTTGG ACAAACGTGA CACTTTTATC 
GAAAACTTCC TAGCCAAATG TTTAGATTAT GAGTTGAACC ATGACGCATT TGAAATTTGG 
AGCACCTTGG CATGGTGGTT GCCTGCAGTC GTTGATTTAA GAAGGTCTAA AACTTTTTTT 
TGCCATTTTA TCAACGCCGA TGGCATGTTT GACCGTGAAT CTGATGCAGC CACACATCAA 
ACCTCCAAAA TTTACATGCT AAGAAGTATC TTGATGAGCA TGGAATTTAG AGCCCCAGAT 
GTTGGTAAGC TATTTGATGA GTTGGTATTT GATCACCCAT ACGATCAGGT TCGCCAGGCT 
GTCGCTAAAC TATTGACGAC CTTAGTTCAA AATCAAAGTA ATCCGTCAAT TTCAGATCCA 
ACCACATTAT TAGAAGCAGA ACGGAATGAT CCAGATGGAT TAGGGTTACC ATTAAAAAGC 
GTCCCAGAAA AGGTGGATGC CTATATTAAG AAGCAGTTTG AGATCATTAA AAATCTGGAA 
GATTCCGTTG TCGGCCTGAA TCCTCAACAA TTTATTAAGA CAGACTATTT TTACAGGACA 
TCCACAATAT TTTATTGGAT CAAAGAAATG GCGAGAGGGC CTAATAAAGT TTTGTTAGTT 
CCATATTTAG TCGATTATGT TTTACCATTT CTGATCGGAT TAGTGAAACA TAAAGATGTT 
TGTGCTCTTG CCAGTTTAGA TCCTGTTCGA TTGTATGCCG GCTTGGGCTA TATGCCGATC 
AGAAAGAACC ATGTTGCCGC CATTGTTGAC TATGTTTGTT CGTCAAATGT AGCACTATCT 
TCAAACCAAA CGAAACTACA GCTAGCTTTC ATCCAACACT TTTTATCAGC GGAACTGCTT 
CAACTTACTG AGGAAGAAAA GAACAAAATT TTGGAATTTG TTGTCAGCAA TTTGTATAAC 
GAACAGTTTG TAGAGGTGAG AGTTCGTGCG GCATCCATTT TGTCTGATAT CGTCCATAAC 
TGGAAAGAGG AACAGCCGTT GTTGAGTTTA ATCGAGAGAT TTGCAAAGGG TCTCGACGTC 
AACAAATACA CGTCGAAAGA AAGACAAAAG TTATCCAAAA CGGATATCAA GATTCATGGT 
AACGTTTTGG GTTTGGGAGC TATTATATCA GCGTTCCCCT ATGTTTTTCC CCTACCGCCA 
TGGATACCCA AGCAACTAAG CAACTTATCC TCGTGGGCCA GGACTAGTGG TATGACTGGA 
CAAGCTGCCA AAAATACTAT TAGTGAGTTC AAGAAGGTGA GGGCCGACAC TTGGAAGTTC 
GATAGAGCAT CTTTCAATAC GGAGGAACTG GAAGACCTGG AGGGTGTCCT ATGGAGAAGT 
TACTATGCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_116648.2
CDS	1..6432
Translation	MTANNDDDIKSPIPITNKTLSQLKRFERSPGRPSSSQGEIKRKKSRLYAADGRPHSPLRARSATPTLQDQKLFNGMDSTSLLNERLQHYTLDYVSDRAQHMKNIYDPSSRWFSRSVRPEFPIEEFLPYKTESHEDQAKYLCHVLVNLYIAISSLDIQGLISISSKDLADLKKEVDDLALKTDLFRLSNNTAENDLLGNDIADYDDAEGLEDELDEYFDLAGPDFNATGKITAKSATIVNVNHWTNELKNCLHFDFPVALRKSLATVYYYLSLVQGQKVYRQMHVDMFERLVSLDDDRTNFTELLQKQGLLLDHQIMLNFLCEFLPYPDPDYARYELSSKEDLQLFRLLLKHAHNAKPFFDKSKESLLVDTMNFLLSSLAPSTMMAVMPIVTSVVPYHYHIHSKIIDYFPFCYSIWSSVSANVAIDTHMYDFVGSISKDVHNKILSSEHEKDVVGVEFGEFGIFTDDQMTFMFNRLQGHLRTDGQIHSYSRTVKPFVYAINGSKKDRFFEKLVSLAKAIETFIHPSNNGFWTKPNAKFVHAFIKSYHGRVKYEEDICARGVTNGICLTSFCHEEIVEIFLNIISLGSQNKNPDIANYYISCFAYLLELDPSNAYLIYDKILIDLYDTLADQFINSRHRIISSLKQFTRVIRFIVMDKLYRVHITNVLSMLVSKLDMNDTNLTSNLINGIVSIAAFIPIQDLTGEDDYISFESDTLPLVQQHFYHIKCGESSKTFRVDDELLNNAFKASTTVFQSMLKVYVEKIFQLVDVDLEDSLVTKINQTTMILQESMDDKIFNYFASLLQRNFWSNDSFKEKDPNYELVTIPLAALVRRNNGLSKELVRTLLFHIKEQIKRGAGSVRSTSEIQQRDVKLVLYLTALNDVLRQCHESLLEYSDELITFMKYLYDNVTNPPLDVITSIVIHSALATLCTTEITDCRLFPEDSKIPEKDRWGGLQFDPRRFDKQHLSFQWHVPSSDEITLSISILESLSEYCINNVEELMKAPRHDSEYGDMIQKYVLVMTHTLSGSSLLFDPDFNKYRTQSNLSYREKLILLKNIRENNCDPQELDIDIEQIRSGKDDEDYIESKDIEAGLNAGVSDVVQLRDEFPDELIVDEEVVSEMPSGVNTPIAGTHGTDNSAMSSDLAFRDLDIYTCNYYFGNTTEEKLQNPQYLQVHRVRARIGHFFHKLYVFLSTNFENNTNMFQILLHGLKVWFTDLGQETVFNEDPNAFIDVDFLENVQSLSHVNEPFTRTNFAIRANSLHQSRVLLHSTNRKASKLENLLLVDIIQLATSLYPDIYKPAQGTLVHCMKQLVGSYGVVINKIIPSLEKAIKDHDYMKIQVILNVLLIKKIHRKLMTDYKDIGRLIFLLIECCRVNELEIGMYADKILTDIVIGIKIPSSVCVISDQAFLPLAPPDGTINLQVEAVKLAKKKKREYYLSLLVDLQDKLLDKLDNEKDMGWKIRMFILRFVTQIQSNLESKPDKRAVFSIISQISTKHPEIIHLVVKSLLSTCNKIISLSDYEYDITRAYKNEFNPSFVEILDTSTTSFPKTFTEEMNNFDNPKYFIDLRAYVGWLCWGRLMYVMSPKALKLNLRENELEVLKTAGHLLTREFLRDVTMNLVQDNETRGVFSSGNVSFFSLVILLISSGFCELNMSDLFELCESYYNKDDKASMIMSVEIVAGLVCGSKFMSVSDLDKRDTFIENFLAKCLDYELNHDAFEIWSTLAWWLPAVVDLRRSKTFFCHFINADGMFDRESDAATHQTSKIYMLRSILMSMEFRAPDVGKLFDELVFDHPYDQVRQAVAKLLTTLVQNQSNPSISDPTTLLEAERNDPDGLGLPLKSVPEKVDAYIKKQFEIIKNLEDSVVGLNPQQFIKTDYFYRTSTIFYWIKEMARGPNKVLLVPYLVDYVLPFLIGLVKHKDVCALASLDPVRLYAGLGYMPIRKNHVAAIVDYVCSSNVALSSNQTKLQLAFIQHFLSAELLQLTEEEKNKILEFVVSNLYNEQFVEVRVRAASILSDIVHNWKEEQPLLSLIERFAKGLDVNKYTSKERQKLSKTDIKIHGNVLGLGAIISAFPYVFPLPPWIPKQLSNLSSWARTSGMTGQAAKNTISEFKKVRADTWKFDRASFNTEELEDLEGVLWRSYYA

Target ORF information:

RefSeq Version	NM_001179959.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C Blm10p (BLM10), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001179959.1

ORF Insert Sequence:

ATGACCGCTA ACAATGACGA TGATATCAAA TCACCCATTC CCATTACTAA CAAGACCTTA 
TCCCAATTGA AGCGCTTTGA GAGAAGTCCA GGAAGGCCCA GTTCTTCTCA GGGCGAGATA 
AAACGTAAAA AGTCTAGGCT ATATGCCGCA GACGGAAGAC CACATTCTCC GCTAAGAGCA 
AGGTCTGCTA CCCCAACGCT ACAGGACCAA AAACTGTTCA ATGGCATGGA TTCCACTTCC 
CTTTTGAATG AAAGGCTACA GCATTATACG CTGGATTATG TTAGTGACAG GGCCCAGCAT 
ATGAAGAATA TATATGACCC ATCATCTCGC TGGTTCAGCA GATCCGTGAG GCCTGAATTT 
CCTATTGAAG AGTTCTTACC ATATAAGACT GAAAGTCATG AAGATCAAGC GAAATACTTG 
TGCCATGTCT TAGTTAATCT TTATATTGCG ATCAGCTCAT TAGATATACA AGGTTTGATT 
TCTATTTCCA GTAAAGATCT GGCTGATTTA AAGAAAGAAG TGGATGATTT AGCTCTTAAA 
ACCGATCTTT TCAGGTTATC TAACAACACA GCGGAGAATG ACTTACTTGG TAACGATATT 
GCTGATTATG ACGATGCGGA AGGCCTGGAG GACGAATTGG ATGAATACTT CGACTTAGCA 
GGCCCCGACT TTAATGCTAC CGGAAAAATC ACAGCTAAAT CAGCTACTAT TGTGAATGTA 
AACCATTGGA CTAATGAGCT CAAAAATTGT CTACATTTTG ATTTTCCAGT AGCTTTAAGA 
AAGTCGCTAG CGACAGTTTA TTATTATTTG TCTCTTGTTC AGGGCCAAAA GGTGTATAGA 
CAAATGCATG TCGATATGTT CGAAAGATTA GTAAGCCTTG ACGATGATAG GACAAATTTC 
ACTGAACTGT TGCAAAAACA AGGTCTTTTG CTAGATCATC AAATCATGCT CAATTTCCTG 
TGCGAATTTC TACCTTATCC AGATCCCGAC TATGCTCGTT ATGAATTATC ATCAAAGGAA 
GATTTACAGT TATTTAGATT ACTTTTGAAG CATGCACATA ATGCAAAACC ATTTTTCGAT 
AAGTCAAAGG AAAGTTTGTT AGTTGATACG ATGAATTTTC TGTTGTCTAG TCTTGCACCA 
TCTACTATGA TGGCTGTCAT GCCTATCGTT ACATCCGTTG TGCCCTATCA TTATCATATC 
CATTCTAAGA TCATCGATTA TTTCCCGTTT TGCTATAGCA TCTGGAGCTC AGTCAGCGCA 
AACGTGGCCA TCGACACTCA CATGTATGAT TTTGTTGGGT CAATTTCCAA AGACGTTCAT 
AATAAGATTT TGAGTAGCGA GCATGAAAAG GATGTAGTTG GAGTGGAGTT TGGAGAATTC 
GGGATTTTTA CTGATGATCA AATGACTTTT ATGTTCAATA GGTTACAAGG CCATCTTAGA 
ACAGACGGTC AAATACATTC GTATTCCCGC ACAGTGAAGC CTTTTGTTTA TGCTATAAAC 
GGATCAAAAA AAGATAGGTT TTTTGAAAAA CTTGTAAGTT TAGCCAAAGC AATCGAAACA 
TTTATCCATC CCTCTAATAA TGGGTTTTGG ACCAAGCCAA ATGCTAAATT CGTTCATGCA 
TTTATAAAGT CTTACCACGG AAGGGTCAAA TATGAAGAAG ATATTTGTGC TAGGGGCGTC 
ACAAATGGGA TATGTTTAAC TTCTTTTTGT CACGAAGAGA TAGTTGAAAT ATTCTTAAAT 
ATTATCAGTC TGGGTTCACA GAATAAAAAT CCTGATATTG CGAACTATTA CATCTCTTGT 
TTCGCATATC TGTTAGAGCT GGATCCTTCA AATGCATATT TAATTTATGA CAAAATACTG 
ATAGATTTGT ACGATACGCT GGCTGACCAA TTTATCAATT CGAGACACAG AATCATTTCC 
TCTTTGAAAC AATTTACAAG AGTAATTCGG TTTATTGTGA TGGATAAGCT ATATCGCGTG 
CACATAACAA ACGTCCTTTC GATGCTGGTC TCCAAACTTG ATATGAACGA TACTAATTTG 
ACAAGCAACC TCATCAACGG TATTGTATCT ATAGCCGCTT TCATTCCTAT CCAAGACCTC 
ACTGGGGAGG ACGATTATAT ATCGTTTGAA TCGGATACTC TTCCTTTAGT TCAACAACAT 
TTTTATCATA TTAAGTGTGG CGAAAGTTCA AAGACCTTCC GAGTTGATGA TGAACTGTTA 
AATAACGCTT TCAAAGCTTC CACTACAGTT TTTCAAAGTA TGCTAAAAGT ATACGTAGAA 
AAAATTTTCC AATTGGTTGA TGTAGACTTA GAGGACTCTT TGGTTACTAA AATAAACCAA 
ACAACTATGA TTTTACAAGA GTCTATGGAC GATAAAATAT TTAATTATTT TGCTTCTTTA 
TTACAGAGAA ACTTCTGGAG TAATGACTCC TTCAAGGAAA AGGATCCAAA CTATGAATTA 
GTTACTATCC CACTAGCGGC TTTAGTAAGA AGGAATAATG GTTTGAGTAA AGAGTTGGTC 
AGAACTCTTT TATTCCATAT CAAAGAACAA ATCAAAAGAG GCGCCGGGTC TGTAAGAAGT 
ACTTCAGAGA TTCAACAGAG GGATGTTAAG TTAGTTTTAT ACTTAACTGC ACTAAATGAT 
GTCTTAAGGC AATGTCATGA ATCTCTATTG GAGTATAGCG ATGAGTTGAT AACATTCATG 
AAATATTTAT ACGACAACGT CACTAACCCG CCATTGGATG TTATCACATC TATTGTTATT 
CACAGTGCTT TAGCAACTCT ATGTACAACC GAAATAACTG ATTGTCGTCT ATTCCCAGAG 
GACTCTAAGA TTCCGGAAAA AGACAGATGG GGAGGACTAC AGTTCGATCC TCGAAGATTT 
GATAAACAGC ATTTGAGTTT TCAGTGGCAC GTACCTTCTA GTGACGAGAT AACTTTATCC 
ATAAGCATTC TAGAAAGCCT TTCCGAATAC TGTATTAACA ACGTAGAAGA ACTGATGAAA 
GCTCCAAGAC ATGATTCCGA ATATGGCGAT ATGATACAAA AATATGTTTT AGTTATGACA 
CATACGCTTT CCGGGTCAAG TTTACTTTTT GATCCAGATT TTAACAAATA TAGGACGCAA 
TCAAACTTAT CATACAGAGA GAAACTGATT TTATTAAAGA ATATACGCGA AAATAATTGT 
GACCCTCAAG AACTGGATAT TGATATTGAA CAAATTCGTT CTGGCAAGGA TGATGAAGAC 
TATATTGAGA GTAAGGACAT TGAAGCAGGG CTGAATGCAG GAGTTTCCGA TGTTGTGCAG 
TTAAGAGATG AGTTTCCGGA TGAATTAATT GTTGATGAAG AAGTAGTGTC TGAGATGCCA 
TCTGGTGTAA ATACCCCTAT CGCGGGGACG CATGGCACGG ACAATTCAGC TATGAGTTCG 
GATCTAGCTT TCAGGGATTT AGATATTTAC ACCTGTAATT ATTACTTCGG AAATACCACT 
GAGGAGAAGT TACAAAACCC ACAATATTTA CAAGTCCACA GAGTTAGAGC GCGCATTGGA 
CATTTCTTTC ACAAACTCTA TGTTTTTTTA TCTACAAACT TCGAAAACAA CACCAACATG 
TTCCAAATTC TATTGCACGG ATTGAAAGTT TGGTTCACAG ATCTGGGACA AGAAACGGTC 
TTCAATGAAG ACCCAAATGC CTTCATTGAC GTTGATTTCC TAGAAAACGT TCAATCTCTC 
TCACACGTAA ATGAGCCCTT CACGAGAACC AATTTTGCAA TCAGAGCAAA CAGTTTGCAC 
CAGAGTAGAG TTCTATTACA TTCAACAAAT AGAAAAGCTT CCAAGCTGGA AAACCTATTG 
TTAGTTGACA TCATACAGTT AGCGACATCC CTTTATCCTG ATATTTATAA ACCAGCACAG 
GGAACTTTGG TACACTGTAT GAAACAATTA GTTGGGTCAT ATGGCGTAGT CATCAATAAA 
ATTATTCCAT CATTAGAGAA AGCGATTAAG GATCATGATT ATATGAAAAT CCAAGTTATT 
TTAAATGTTT TGTTAATTAA GAAAATCCAT AGGAAGCTTA TGACGGATTA TAAAGACATC 
GGCAGATTGA TATTTCTGCT TATTGAATGT TGTCGTGTGA ATGAATTAGA GATTGGTATG 
TATGCAGATA AAATCTTAAC TGACATAGTG ATTGGGATCA AGATTCCTTC TAGTGTATGT 
GTCATTTCCG ATCAAGCTTT CTTACCTTTA GCACCTCCTG ATGGTACTAT TAATTTGCAA 
GTTGAAGCGG TAAAGCTTGC CAAAAAGAAA AAGCGTGAGT ACTACCTCTC TCTGTTAGTG 
GATTTGCAGG ACAAACTTTT AGACAAATTA GATAATGAAA AAGATATGGG GTGGAAGATA 
AGAATGTTCA TTTTACGTTT TGTTACACAA ATCCAATCGA ACCTCGAAAG CAAACCCGAT 
AAAAGAGCAG TATTTTCAAT AATCTCCCAA ATCTCCACAA AACATCCCGA AATCATACAT 
TTGGTTGTAA AGTCATTGTT GTCGACGTGC AACAAGATAA TATCTCTCTC TGACTATGAA 
TATGACATCA CCAGGGCCTA TAAGAATGAA TTCAATCCAT CATTTGTGGA AATACTGGAC 
ACTTCGACCA CAAGCTTCCC TAAAACGTTT ACTGAAGAAA TGAATAACTT TGATAACCCC 
AAGTATTTTA TCGATTTGAG GGCGTATGTA GGGTGGCTAT GTTGGGGAAG GCTTATGTAC 
GTCATGTCGC CGAAAGCTTT AAAGCTCAAT TTACGTGAGA ATGAACTGGA AGTCCTCAAG 
ACAGCTGGTC ATCTATTGAC AAGAGAATTC CTGAGAGATG TTACAATGAA TTTAGTCCAA 
GATAATGAAA CTAGGGGTGT TTTTAGTAGT GGTAACGTGT CATTTTTCTC TTTAGTAATC 
CTTTTGATAT CATCTGGTTT CTGCGAACTG AATATGTCGG ATCTCTTTGA GCTATGTGAA 
TCCTACTATA ACAAAGACGA TAAGGCTTCG ATGATCATGT CTGTCGAGAT AGTGGCTGGC 
TTAGTTTGCG GGAGTAAGTT TATGTCAGTC TCTGACTTGG ACAAACGTGA CACTTTTATC 
GAAAACTTCC TAGCCAAATG TTTAGATTAT GAGTTGAACC ATGACGCATT TGAAATTTGG 
AGCACCTTGG CATGGTGGTT GCCTGCAGTC GTTGATTTAA GAAGGTCTAA AACTTTTTTT 
TGCCATTTTA TCAACGCCGA TGGCATGTTT GACCGTGAAT CTGATGCAGC CACACATCAA 
ACCTCCAAAA TTTACATGCT AAGAAGTATC TTGATGAGCA TGGAATTTAG AGCCCCAGAT 
GTTGGTAAGC TATTTGATGA GTTGGTATTT GATCACCCAT ACGATCAGGT TCGCCAGGCT 
GTCGCTAAAC TATTGACGAC CTTAGTTCAA AATCAAAGTA ATCCGTCAAT TTCAGATCCA 
ACCACATTAT TAGAAGCAGA ACGGAATGAT CCAGATGGAT TAGGGTTACC ATTAAAAAGC 
GTCCCAGAAA AGGTGGATGC CTATATTAAG AAGCAGTTTG AGATCATTAA AAATCTGGAA 
GATTCCGTTG TCGGCCTGAA TCCTCAACAA TTTATTAAGA CAGACTATTT TTACAGGACA 
TCCACAATAT TTTATTGGAT CAAAGAAATG GCGAGAGGGC CTAATAAAGT TTTGTTAGTT 
CCATATTTAG TCGATTATGT TTTACCATTT CTGATCGGAT TAGTGAAACA TAAAGATGTT 
TGTGCTCTTG CCAGTTTAGA TCCTGTTCGA TTGTATGCCG GCTTGGGCTA TATGCCGATC 
AGAAAGAACC ATGTTGCCGC CATTGTTGAC TATGTTTGTT CGTCAAATGT AGCACTATCT 
TCAAACCAAA CGAAACTACA GCTAGCTTTC ATCCAACACT TTTTATCAGC GGAACTGCTT 
CAACTTACTG AGGAAGAAAA GAACAAAATT TTGGAATTTG TTGTCAGCAA TTTGTATAAC 
GAACAGTTTG TAGAGGTGAG AGTTCGTGCG GCATCCATTT TGTCTGATAT CGTCCATAAC 
TGGAAAGAGG AACAGCCGTT GTTGAGTTTA ATCGAGAGAT TTGCAAAGGG TCTCGACGTC 
AACAAATACA CGTCGAAAGA AAGACAAAAG TTATCCAAAA CGGATATCAA GATTCATGGT 
AACGTTTTGG GTTTGGGAGC TATTATATCA GCGTTCCCCT ATGTTTTTCC CCTACCGCCA 
TGGATACCCA AGCAACTAAG CAACTTATCC TCGTGGGCCA GGACTAGTGG TATGACTGGA 
CAAGCTGCCA AAAATACTAT TAGTGAGTTC AAGAAGGTGA GGGCCGACAC TTGGAAGTTC 
GATAGAGCAT CTTTCAATAC GGAGGAACTG GAAGACCTGG AGGGTGTCCT ATGGAGAAGT 
TACTATGCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

Analysis of the nucleotide sequence of chromosome VI from Saccharomyces cerevisiae.
Nature genetics10(3)261-8(1995 Jul)
Murakami Y,Naitou M,Hagiwara H,Shibata T,Ozawa M,Sasanuma S,Sasanuma M,Tsuchiya Y,Soeda E,Yokoyama K

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BLM10 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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