SMC1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	SMC1
Entrez Gene ID	850540
Full Name	cohesin subunit SMC1
Synonyms	CHL10
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001179958.1	NP_116647.1	cohesin subunit SMC1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	SMC1A	XP_538049.3
Danio rerio (zebrafish)	smc1a	NP_001155103.1
Schizosaccharomyces pombe (fission yeast)	psm1	NP_596049.2
Mus musculus (house mouse)	Smc1a	NP_062684.2
Caenorhabditis elegans (roundworm)	him-1	NP_001040658.2
	SMC1A	XP_001091228.1
Arabidopsis thaliana (thale cress)	TTN8	NP_001190092.1
Oryza sativa (rice)	Os12g0641500	NP_001067404.1
Danio rerio (zebrafish)	smc1al	NP_997975.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002947	XP_311953.3
Saccharomyces cerevisiae (baker's yeast)	SMC1	NP_116647.1
Kluyveromyces lactis	KLLA0D07502g	XP_453397.1
Eremothecium gossypii	AGOS_AGL023W	NP_986643.1
Homo sapiens (human)	SMC1A	NP_006297.2
Pan troglodytes (chimpanzee)	SMC1A	XP_003954052.1
Bos taurus (cattle)	SMC1A	NP_777039.1
Rattus norvegicus (Norway rat)	Smc1a	NP_113871.1
Gallus gallus (chicken)	SMC1A	NP_989847.1
Drosophila melanogaster (fruit fly)	SMC1	NP_651211.2
Magnaporthe oryzae (rice blast fungus)	MGG_16203	XP_003709622.1
Neurospora crassa	NCU01323	XP_961409.2
Xenopus tropicalis (tropical clawed frog)	LOC100493711	XP_002935560.1

Gene Ontology
Bibliography

Related articles in PubMed

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

Analysis of the nucleotide sequence of chromosome VI from Saccharomyces cerevisiae.
Murakami Y, Naitou M, Hagiwara H, Shibata T, Ozawa M, Sasanuma S, Sasanuma M, Tsuchiya Y, Soeda E, Yokoyama K
Nature genetics10(3)261-8(1995 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

A reversible association between Smc1 and Smc3 coiled coils is regulated by lysine acetylation and is required for cohesin association with the DNA.
Title: A Reversible Association between Smc Coiled Coils Is Regulated by Lysine Acetylation and Is Required for Cohesin Association with the DNA.

We identified a large number of mutations in the CC region of both Smc1 and Smc3... we introduced them to the yeast Smc1 and Smc3 CC domains and characterized the effect of these mutant alleles on cohesin's function. We identified a missense mutation in the region of the kink domain of Smc3, which was previously identified in kidney carcinoma
Title: Identification of a region in the coiled-coil domain of Smc3 that is essential for cohesin activity.

These results also suggest that Eco1 promotes cohesion by modulating the ATPase cycle of DNA-bound cohesin in a state that is permissive for DNA tethering and refractory to Wpl1 inhibition.
Title: The ATPases of cohesin interface with regulators to modulate cohesin-mediated DNA tethering.

Smc5/6 and cohesin complexes are interrelated and cooperating factors that control chromosome dynamics throughout interphase. [review]
Title: Functional interplay between cohesin and Smc5/6 complexes.

by retarding Topo II-driven decatenation, cohesin (Smc1, Smc3, Scc1) mediates sister chromatid cohesion by an indirect mechanism as well as one involving entrapment of sister DNAs inside its tripartite ring
Title: Cohesin's concatenation of sister DNAs maintains their intertwining.

The following SMC1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SMC1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi00228	NM_001179958.1 Latest version!	Saccharomyces cerevisiae S288C cohesin subunit SMC1 (SMC1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SMC1 ( NM_001179958.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_116647.1 Saccharomyces cerevisiae S288C cohesin subunit SMC1 (SMC1), partial mRNA.

Price & Availability↑

CloneID	OSi00228
Clone ID Related Accession (Same CDS sequence)	NM_001179958.1
Accession Version	NM_001179958.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3678bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	cohesin subunit SMC1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001138). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGGGACGTT TAGTTGGCTT AGAACTAAGT AATTTCAAGT CCTATAGAGG CGTTACCAAG 
GTAGGATTCG GCGAGTCGAA TTTCACAAGT ATTATCGGTC CTAACGGATC TGGTAAATCG 
AATATGATGG ATGCTATCTC ATTCGTACTC GGTGTGCGGA GTAATCATTT GAGGTCAAAC 
ATCTTGAAAG ATTTAATCTA TAGAGGTGTT CTAAACGATG AGAATAGTGA CGATTATGAT 
AACGAGGGCG CTGCCTCTTC GAACCCACAA TCCGCATACG TGAAGGCATT TTATCAAAAA 
GGTAACAAAC TGGTGGAGCT GATGAGGATA ATTTCCAGAA ACGGTGACAC TAGTTATAAA 
ATTGATGGAA AAACTGTCTC CTATAAGGAC TATTCTATAT TTCTTGAGAA CGAAAATATT 
CTTATCAAAG CCAAAAATTT TCTAGTGTTC CAGGGTGATG TTGAGCAAAT TGCAGCACAA 
TCTCCCGTAG AATTATCAAG AATGTTTGAA GAAGTATCAG GTTCTATCCA ATACAAAAAG 
GAGTATGAAG AGTTGAAGGA AAAGATTGAG AAATTAAGCA AATCTGCAAC CGAATCTATA 
AAAAATAGGA GAAGGATCCA TGGAGAATTG AAGACATATA AAGAAGGTAT CAATAAGAAC 
GAGGAGTATA GGAAACAATT GGACAAAAAA AATGAATTAC AGAAGTTCCA GGCTCTATGG 
CAGTTATATC ATTTAGAGCA ACAAAAAGAG GAGCTAACGG ACAAGCTGTC CGCATTAAAC 
TCTGAAATAT CGTCTTTAAA GGGAAAAATA AATAACGAGA TGAAATCATT ACAACGCTCA 
AAATCTTCCT TTGTTAAAGA AAGCGCAGTA ATTTCTAAGC AAAAAAGTAA ATTAGATTAT 
ATCTTCAAGG ATAAGGAAAA ATTAGTCTCG GATTTACGGC TCATAAAGGT TCCTCAACAG 
GCAGCAGGGA AGCGAATTTC CCATATAGAA AAAAGAATCG AAAGTTTACA GAAAGATCTT 
CAAAGACAGA AGACTTATGT GGAGAGATTT GAAACACAAC TAAAAGTGGT GACCAGATCA 
AAGGAAGCTT TTGAAGAGGA AATCAAACAA TCTGCTAGAA ACTATGACAA ATTCAAGCTA 
AATGAAAATG ATTTAAAGAC ATATAATTGC TTACATGAAA AATATCTGAC TGAAGGTGGG 
TCAATCCTAG AAGAAAAAAT TGCCGTTTTG AACAACGATA AGCGAGAAAT CCAAGAGGAA 
TTGGAGAGAT TCAACAAAAG GGCAGATATT TCTAAAAGAA GGATAACGGA GGAGCTTTCT 
ATAACAGGAG AAAAATTGGA CACGCAATTA AACGATTTAA GAGTTTCTTT GAATGAGAAA 
AACGCCCTTC ATACTGAACG TTTGCACGAG CTGAAAAAAT TACAATCTGA TATTGAATCT 
GCTAATAATC AAGAATACGA CTTAAATTTC AAGTTGAGGG AAACGTTGGT TAAGATCGAT 
GACTTGAGTG CTAATCAAAG AGAAACAATG AAAGAAAGAA AACTAAGAGA AAATATAGCA 
ATGTTGAAAA GATTCTTCCC CGGAGTAAAA GGTCTTGTTC ATGATCTTTG TCACCCAAAA 
AAGGAGAAAT ATGGCTTGGC AGTGTCTACC ATCTTAGGTA AGAACTTTGA TTCCGTCATT 
GTAGAAAATT TAACCGTAGC TCAAGAATGC ATTGCATTTT TGAAGAAGCA ACGTGCGGGC 
ACTGCATCTT TCATACCACT AGACACAATT GAGACAGAGT TACCTACATT ATCATTGCCT 
GACTCACAAG ACTATATTTT ATCAATTAAT GCTATTGACT ACGAGCCGGA ATATGAAAAA 
GCGATGCAAT ATGTGTGTGG CGATTCCATC ATATGTAATA CGTTGAATAT TGCTAAAGAT 
CTGAAATGGA AAAAGGGCAT AAGAGGCAAA TTGGTTACAA TTGAAGGTGC TTTGATCCAC 
AAGGCCGGTT TGATGACAGG TGGTATATCA GGAGATGCCA ATAATAGGTG GGATAAAGAA 
GAATATCAAA GCTTAATGTC TTTAAAAGAC AAATTACTAA TCCAAATCGA TGAACTTTCC 
AACGGTCAAC GCTCTAATTC AATCAGAGCA AGAGAAGTTG AAAATAGTGT TTCACTATTG 
AACTCGGACA TAGCAAATTT GAGAACTCAA GTAACACAAC AAAAACGCTC CTTGGATGAA 
AATCGTTTAG AGATTAAGTA CCATAATGAC TTGATAGAGA AAGAAATTCA ACCGAAGATA 
ACTGAACTAA AGAAGAAGCT AGATGATTTA GAAAATACTA AAGATAATTT AGTGAAAGAG 
AAGGAGGCTT TACAAAATAA TATTTTCAAA GAATTCACTA GTAAAATTGG CTTTACAATC 
AAAGAATATG AAAATCATTC CGGTGAATTG ATGAGACAAC AATCTAAAGA ATTACAGCAG 
TTACAAAAAC AAATTTTGAC CGTTGAAAAT AAGTTGCAGT TTGAGACAGA CAGACTAAGT 
ACTACTCAAA GAAGATATGA AAAGGCGCAA AAGGATTTAG AGAATGCTCA AGTTGAAATG 
AAGTCTTTGG AAGAACAGGA ATATGCAATA GAAATGAAAA TCGGATCAAT AGAGTCTAAA 
TTGGAAGAAC ACAAAAATCA CTTAGATGAG TTACAGAAGA AATTTGTAAC GAAGCAAAGT 
GAATTAAATT CCAGCGAAGA TATTCTAGAG GACATGAACA GCAACTTACA AGTCTTAAAA 
AGGGAAAGAG ACGGTATAAA GGAAGATATT GAAAAGTTTG ATTTAGAGAG AGTAACAGCG 
TTAAAGAATT GTAAAATTTC TAATATAAAT ATACCTATAT CATCTGAAAC AACGATAGAT 
GATTTACCAA TATCTTCCAC TGATAATGAA GCAATTACAA TTTCCAACAG TATCGATATA 
AACTATAAAG GACTACCTAA AAAATACAAA GAAAACAATA CCGATTCGGC AAGGAAGGAG 
CTGGAGCAGA AGATTCATGA AGTGGAGGAA ATATTGAACG AGTTGCAGCC CAATGCAAGA 
GCTTTGGAGA GATACGACGA GGCGGAAGGA AGGTTTGAAG TGATTAATAA CGAAACAGAA 
CAACTAAAGG CCGAAGAAAA GAAAATATTA AACCAGTTCC TAAAAATTAA GAAAAAAAGA 
AAGGAACTGT TCGAAAAGAC ATTTGATTAT GTGAGCGACC ATTTAGACGC AATCTACAGG 
GAACTTACTA AAAATCCCAA CTCCAATGTG GAATTGGCCG GTGGTAACGC TTCTTTAACC 
ATAGAAGACG AAGATGAACC GTTCAATGCG GGAATCAAAT ATCATGCCAC TCCGCCTCTT 
AAAAGATTCA AAGACATGGA ATATCTTTCT GGTGGTGAAA AAACCGTAGC TGCATTAGCT 
CTATTATTTG CTATTAATTC CTACCAGCCT AGTCCCTTCT TCGTGCTGGA CGAAGTGGAC 
GCAGCCCTAG ACATTACTAA CGTCCAGAGA ATTGCTGCCT ATATAAGAAG GCACCGTAAT 
CCAGATCTCC AATTCATTGT CATTTCATTG AAGAACACCA TGTTTGAAAA ATCTGACGCT 
CTCGTTGGGG TTTACAGACA GCAACAAGAA AACTCGTCGA AGATCATAAC TTTGGACTTG 
AGCAATTACG CAGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_116647.1
CDS	1..3678
Translation	MGRLVGLELSNFKSYRGVTKVGFGESNFTSIIGPNGSGKSNMMDAISFVLGVRSNHLRSNILKDLIYRGVLNDENSDDYDNEGAASSNPQSAYVKAFYQKGNKLVELMRIISRNGDTSYKIDGKTVSYKDYSIFLENENILIKAKNFLVFQGDVEQIAAQSPVELSRMFEEVSGSIQYKKEYEELKEKIEKLSKSATESIKNRRRIHGELKTYKEGINKNEEYRKQLDKKNELQKFQALWQLYHLEQQKEELTDKLSALNSEISSLKGKINNEMKSLQRSKSSFVKESAVISKQKSKLDYIFKDKEKLVSDLRLIKVPQQAAGKRISHIEKRIESLQKDLQRQKTYVERFETQLKVVTRSKEAFEEEIKQSARNYDKFKLNENDLKTYNCLHEKYLTEGGSILEEKIAVLNNDKREIQEELERFNKRADISKRRITEELSITGEKLDTQLNDLRVSLNEKNALHTERLHELKKLQSDIESANNQEYDLNFKLRETLVKIDDLSANQRETMKERKLRENIAMLKRFFPGVKGLVHDLCHPKKEKYGLAVSTILGKNFDSVIVENLTVAQECIAFLKKQRAGTASFIPLDTIETELPTLSLPDSQDYILSINAIDYEPEYEKAMQYVCGDSIICNTLNIAKDLKWKKGIRGKLVTIEGALIHKAGLMTGGISGDANNRWDKEEYQSLMSLKDKLLIQIDELSNGQRSNSIRAREVENSVSLLNSDIANLRTQVTQQKRSLDENRLEIKYHNDLIEKEIQPKITELKKKLDDLENTKDNLVKEKEALQNNIFKEFTSKIGFTIKEYENHSGELMRQQSKELQQLQKQILTVENKLQFETDRLSTTQRRYEKAQKDLENAQVEMKSLEEQEYAIEMKIGSIESKLEEHKNHLDELQKKFVTKQSELNSSEDILEDMNSNLQVLKRERDGIKEDIEKFDLERVTALKNCKISNINIPISSETTIDDLPISSTDNEAITISNSIDINYKGLPKKYKENNTDSARKELEQKIHEVEEILNELQPNARALERYDEAEGRFEVINNETEQLKAEEKKILNQFLKIKKKRKELFEKTFDYVSDHLDAIYRELTKNPNSNVELAGGNASLTIEDEDEPFNAGIKYHATPPLKRFKDMEYLSGGEKTVAALALLFAINSYQPSPFFVLDEVDAALDITNVQRIAAYIRRHRNPDLQFIVISLKNTMFEKSDALVGVYRQQQENSSKIITLDLSNYAE

Target ORF information:

RefSeq Version	NM_001179958.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C cohesin subunit SMC1 (SMC1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001179958.1

ORF Insert Sequence:

ATGGGACGTT TAGTTGGCTT AGAACTAAGT AATTTCAAGT CCTATAGAGG CGTTACCAAG 
GTAGGATTCG GCGAGTCGAA TTTCACAAGT ATTATCGGTC CTAACGGATC TGGTAAATCG 
AATATGATGG ATGCTATCTC ATTCGTACTC GGTGTGCGGA GTAATCATTT GAGGTCAAAC 
ATCTTGAAAG ATTTAATCTA TAGAGGTGTT CTAAACGATG AGAATAGTGA CGATTATGAT 
AACGAGGGCG CTGCCTCTTC GAACCCACAA TCCGCATACG TGAAGGCATT TTATCAAAAA 
GGTAACAAAC TGGTGGAGCT GATGAGGATA ATTTCCAGAA ACGGTGACAC TAGTTATAAA 
ATTGATGGAA AAACTGTCTC CTATAAGGAC TATTCTATAT TTCTTGAGAA CGAAAATATT 
CTTATCAAAG CCAAAAATTT TCTAGTGTTC CAGGGTGATG TTGAGCAAAT TGCAGCACAA 
TCTCCCGTAG AATTATCAAG AATGTTTGAA GAAGTATCAG GTTCTATCCA ATACAAAAAG 
GAGTATGAAG AGTTGAAGGA AAAGATTGAG AAATTAAGCA AATCTGCAAC CGAATCTATA 
AAAAATAGGA GAAGGATCCA TGGAGAATTG AAGACATATA AAGAAGGTAT CAATAAGAAC 
GAGGAGTATA GGAAACAATT GGACAAAAAA AATGAATTAC AGAAGTTCCA GGCTCTATGG 
CAGTTATATC ATTTAGAGCA ACAAAAAGAG GAGCTAACGG ACAAGCTGTC CGCATTAAAC 
TCTGAAATAT CGTCTTTAAA GGGAAAAATA AATAACGAGA TGAAATCATT ACAACGCTCA 
AAATCTTCCT TTGTTAAAGA AAGCGCAGTA ATTTCTAAGC AAAAAAGTAA ATTAGATTAT 
ATCTTCAAGG ATAAGGAAAA ATTAGTCTCG GATTTACGGC TCATAAAGGT TCCTCAACAG 
GCAGCAGGGA AGCGAATTTC CCATATAGAA AAAAGAATCG AAAGTTTACA GAAAGATCTT 
CAAAGACAGA AGACTTATGT GGAGAGATTT GAAACACAAC TAAAAGTGGT GACCAGATCA 
AAGGAAGCTT TTGAAGAGGA AATCAAACAA TCTGCTAGAA ACTATGACAA ATTCAAGCTA 
AATGAAAATG ATTTAAAGAC ATATAATTGC TTACATGAAA AATATCTGAC TGAAGGTGGG 
TCAATCCTAG AAGAAAAAAT TGCCGTTTTG AACAACGATA AGCGAGAAAT CCAAGAGGAA 
TTGGAGAGAT TCAACAAAAG GGCAGATATT TCTAAAAGAA GGATAACGGA GGAGCTTTCT 
ATAACAGGAG AAAAATTGGA CACGCAATTA AACGATTTAA GAGTTTCTTT GAATGAGAAA 
AACGCCCTTC ATACTGAACG TTTGCACGAG CTGAAAAAAT TACAATCTGA TATTGAATCT 
GCTAATAATC AAGAATACGA CTTAAATTTC AAGTTGAGGG AAACGTTGGT TAAGATCGAT 
GACTTGAGTG CTAATCAAAG AGAAACAATG AAAGAAAGAA AACTAAGAGA AAATATAGCA 
ATGTTGAAAA GATTCTTCCC CGGAGTAAAA GGTCTTGTTC ATGATCTTTG TCACCCAAAA 
AAGGAGAAAT ATGGCTTGGC AGTGTCTACC ATCTTAGGTA AGAACTTTGA TTCCGTCATT 
GTAGAAAATT TAACCGTAGC TCAAGAATGC ATTGCATTTT TGAAGAAGCA ACGTGCGGGC 
ACTGCATCTT TCATACCACT AGACACAATT GAGACAGAGT TACCTACATT ATCATTGCCT 
GACTCACAAG ACTATATTTT ATCAATTAAT GCTATTGACT ACGAGCCGGA ATATGAAAAA 
GCGATGCAAT ATGTGTGTGG CGATTCCATC ATATGTAATA CGTTGAATAT TGCTAAAGAT 
CTGAAATGGA AAAAGGGCAT AAGAGGCAAA TTGGTTACAA TTGAAGGTGC TTTGATCCAC 
AAGGCCGGTT TGATGACAGG TGGTATATCA GGAGATGCCA ATAATAGGTG GGATAAAGAA 
GAATATCAAA GCTTAATGTC TTTAAAAGAC AAATTACTAA TCCAAATCGA TGAACTTTCC 
AACGGTCAAC GCTCTAATTC AATCAGAGCA AGAGAAGTTG AAAATAGTGT TTCACTATTG 
AACTCGGACA TAGCAAATTT GAGAACTCAA GTAACACAAC AAAAACGCTC CTTGGATGAA 
AATCGTTTAG AGATTAAGTA CCATAATGAC TTGATAGAGA AAGAAATTCA ACCGAAGATA 
ACTGAACTAA AGAAGAAGCT AGATGATTTA GAAAATACTA AAGATAATTT AGTGAAAGAG 
AAGGAGGCTT TACAAAATAA TATTTTCAAA GAATTCACTA GTAAAATTGG CTTTACAATC 
AAAGAATATG AAAATCATTC CGGTGAATTG ATGAGACAAC AATCTAAAGA ATTACAGCAG 
TTACAAAAAC AAATTTTGAC CGTTGAAAAT AAGTTGCAGT TTGAGACAGA CAGACTAAGT 
ACTACTCAAA GAAGATATGA AAAGGCGCAA AAGGATTTAG AGAATGCTCA AGTTGAAATG 
AAGTCTTTGG AAGAACAGGA ATATGCAATA GAAATGAAAA TCGGATCAAT AGAGTCTAAA 
TTGGAAGAAC ACAAAAATCA CTTAGATGAG TTACAGAAGA AATTTGTAAC GAAGCAAAGT 
GAATTAAATT CCAGCGAAGA TATTCTAGAG GACATGAACA GCAACTTACA AGTCTTAAAA 
AGGGAAAGAG ACGGTATAAA GGAAGATATT GAAAAGTTTG ATTTAGAGAG AGTAACAGCG 
TTAAAGAATT GTAAAATTTC TAATATAAAT ATACCTATAT CATCTGAAAC AACGATAGAT 
GATTTACCAA TATCTTCCAC TGATAATGAA GCAATTACAA TTTCCAACAG TATCGATATA 
AACTATAAAG GACTACCTAA AAAATACAAA GAAAACAATA CCGATTCGGC AAGGAAGGAG 
CTGGAGCAGA AGATTCATGA AGTGGAGGAA ATATTGAACG AGTTGCAGCC CAATGCAAGA 
GCTTTGGAGA GATACGACGA GGCGGAAGGA AGGTTTGAAG TGATTAATAA CGAAACAGAA 
CAACTAAAGG CCGAAGAAAA GAAAATATTA AACCAGTTCC TAAAAATTAA GAAAAAAAGA 
AAGGAACTGT TCGAAAAGAC ATTTGATTAT GTGAGCGACC ATTTAGACGC AATCTACAGG 
GAACTTACTA AAAATCCCAA CTCCAATGTG GAATTGGCCG GTGGTAACGC TTCTTTAACC 
ATAGAAGACG AAGATGAACC GTTCAATGCG GGAATCAAAT ATCATGCCAC TCCGCCTCTT 
AAAAGATTCA AAGACATGGA ATATCTTTCT GGTGGTGAAA AAACCGTAGC TGCATTAGCT 
CTATTATTTG CTATTAATTC CTACCAGCCT AGTCCCTTCT TCGTGCTGGA CGAAGTGGAC 
GCAGCCCTAG ACATTACTAA CGTCCAGAGA ATTGCTGCCT ATATAAGAAG GCACCGTAAT 
CCAGATCTCC AATTCATTGT CATTTCATTG AAGAACACCA TGTTTGAAAA ATCTGACGCT 
CTCGTTGGGG TTTACAGACA GCAACAAGAA AACTCGTCGA AGATCATAAC TTTGGACTTG 
AGCAATTACG CAGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

Analysis of the nucleotide sequence of chromosome VI from Saccharomyces cerevisiae.
Nature genetics10(3)261-8(1995 Jul)
Murakami Y,Naitou M,Hagiwara H,Shibata T,Ozawa M,Sasanuma S,Sasanuma M,Tsuchiya Y,Soeda E,Yokoyama K

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SMC1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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