BUD27 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	BUD27
Entrez Gene ID	850521
Full Name	Bud27p
Synonyms	URI1
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001179943.1	NP_683715.1	Bud27p

Pathways from BioSystems

Homologies

Saccharomyces cerevisiae (baker's yeast)	BUD27	NP_683715.1
Eremothecium gossypii	AGOS_AFR519C	NP_986066.2

Gene Ontology
Bibliography

Related articles in PubMed

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

Analysis of the nucleotide sequence of chromosome VI from Saccharomyces cerevisiae.
Murakami Y, Naitou M, Hagiwara H, Shibata T, Ozawa M, Sasanuma S, Sasanuma M, Tsuchiya Y, Soeda E, Yokoyama K
Nature genetics10(3)261-8(1995 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Yeast Bud27 modulates the biogenesis of Rpc128 and Rpc160 subunits and the assembly of RNA polymerase III.
Title: Yeast Bud27 modulates the biogenesis of Rpc128 and Rpc160 subunits and the assembly of RNA polymerase III.

The data suggest that Bud27 contributes to the folding of Rpb5 within the RNA polymerase II thereby modulating its activity, and participates in the correct assembly of other chromatin-associated factors, such as the RSC complex.
Title: The yeast prefoldin-like URI-orthologue Bud27 associates with the RSC nucleosome remodeler and modulates transcription.

Our data also suggest that the role of Bud27 in RNA pols biogenesis is independent of the chaperone prefoldin (PFD) complex and of Iwr1
Title: The prefoldin bud27 mediates the assembly of the eukaryotic RNA polymerases in an rpb5-dependent manner.

Together with genetic data, these interactions indicate that Uri1p may coordinate translation initiation and cotranslational quality control.
Title: Yeast Uri1p promotes translation initiation and may provide a link to cotranslational quality control.

The following BUD27 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the BUD27 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi00213	NM_001179943.1 Latest version!	Saccharomyces cerevisiae S288C Bud27p (BUD27), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

BUD27 ( NM_001179943.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_683715.1 Saccharomyces cerevisiae S288C Bud27p (BUD27), partial mRNA.

Price & Availability↑

CloneID	OSi00213
Clone ID Related Accession (Same CDS sequence)	NM_001179943.1
Accession Version	NM_001179943.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2391bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	Bud27p
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001138). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGGATTTGT TAGCGGCCAG CGTAGAAAGT ACGTTGAAAA ATCTGCAAGA TAAGAGAAAC 
TTTCTTTCGG AACAAAGAGA GCACTATATA GATATCAGAA GCCGACTGGT TAGATTTATC 
AATGACAATG ATGATGGCGA AGAAGAAGGC GAAGGCCAAG GGATGGTGTT TGGTGATATC 
ATAATTTCTA CGAGTAAGAT ATATCTAAGT CTAGGCTATG AGTATTACGT TGAAAAGACC 
AAAGAGGAGG CCATCACGTT TGTTGATGAT AAATTAAAAC TAATGGAGGA CGCCATTGAG 
CAATTTAACC TGAAGATCGA AGAGGCTAAA AAGACCTTAG ACAACTTGAA TCACATGGAG 
GACGGAAATG GAATTGAAGA AGACGAAGCG AACAACGACG AAGATTTTCT GCCCTCTATG 
GAGATTAGAG AAGAACTAGA TGACGAGGGA AATGTCATAA GTAGTTCCGT TACCCCTACA 
ACAAAGCAAC CATCACAATC GAATTCCAAA AAAGAACAGA CACCTGCAGT CGGTCCAAAG 
GAGAAAGGAT TAGCAAAGGA GAAGAAATCA AAGTCATTCG AAGAAAACTT GAAAGGAAAG 
TTATTGAAAA GGAACGATGA AGTAAAGAAA AAAGTGCAGC CATCTAAAGT AGATACAGAA 
AACGTGTATA CATTTGCCGA CTTGGTCCAA CAGATGGACC AACAAGATGA ATTGGAGGAC 
GGCTATATTG AAACTGATGA AATAAATTAT GATTATGATG CGTTCGAAAA CTCCAATTTC 
AAAGTCAATG ATAACTATGA AGAGGATGAC GAGGACGAAG ATGAAGAAGA GTATCTAAAT 
CATTCTATTA TACCTGGCTT TGAAGCGCAG TCCTCATTTT TACAACAAAT TCAACGCCTA 
CGCGCACAAA AGCAATCCCA AGATCATGAG AGGGAAGAAG GTGACGTTAA TAAATCATTA 
AAACCTATTT TGAAAAAGTC TTCTTTTGCT GAGAATTCAG ACAAAAAGCA GAAAAAGAAA 
CAAGTCGGGT TTGCATCTTC TCTAGAAATT CACGAAGTCG AAAACTTGAA AGAAGAGAAC 
AAGAGGCAGA TGCAATCCTT TGCGGTGCCC ATGTATGAAA CCCAGGAAAG CACAGGTATC 
GCTAATAAAA TGACATCGGA TGAGTTTGAT GGTGATTTAT TTGCTAAGAT GTTGGGTGTC 
CAAGAGGCCG ATGAAGTGCA TGAAAAATAT AAAGAGGAAC TGATAAATCA AGAGAGACTT 
GAAGGGGAAG CAAGCAGGTC GAATAGAAGA ACTAGGGTGT CCAGATTCCG GAAGGATAGA 
GCTTCTAAAA AAGAAAATAC GTTATCAACT TTCAAACAGG AAACGACAAG GTCAGTGGAG 
AACGAGGTAG TGGAAAAAGA GCCTGTTGTG GGGGATATTA TAGAAAAAGA ACCTGTTGTG 
GGGGATGTTA TAGAAAAAGA ACCTGTTGTG GGGGATGTTA TAGAAAAAGA GCCTGCTGTC 
ACCGATATAG TGGAAAGGGA GCCTGCTGTC AACGATATAG TGGAAAGGAA GCCTGTTGTG 
GGGGATATTA TAGAAAAAGA ACCTACTATC AACGATATAG TGGAAAAAGA ACCGGAAATT 
AACAGCAAGA GCGAGTTTGA GACTCCTTTT AAAAAGAAAA AGCTAAAATC ACTGCAAAAA 
CCAAGATCAT CGAAATCAAT GAAAAAAAAA TTTGATCCCA AAATTCTCGA AAATATAAGT 
GATGACGATT ATGATGATGA TGACGATGGT AATAAGAAAC TTTTGTCCAA CAAAAGTAAA 
AATAACACTG ATGAGCAAGA TAAATTTCCT TCAAAAATCC AAGAGGTGTC TAGGTCGATG 
GCTAAGACGG GCGCCACAGT AGGGAGTGAG CCGGTTAGAA TTACAAATGT CGATTATCAC 
GCCCTAGGTG GAAACTTGGA TGATATGGTC AAGGCATATT CATTGGGGTT ATATGACGAC 
GATTTAGAGG AAGATCCAGG AACCATAGTG GAAAAGCTCG AGGATTTCAA AGAATACAAT 
AAGCAGGTGG AGTTGCTGCG TGACGAAATT CGAGACTTTC AACTAGAAAA CAAGCCAGTG 
ACGATGGAGG AGGAGGAAAA TGATGGCAAT GTTATGAATG ATATCATTGA GCATGAATTT 
CCTGAAAGTT ACACAAATGA CGAGGACGAA GTAGCACTAC ATCCGGGCCG GCTACAGGAA 
GAGGTTGCTA TAGAGTACAG ACGGTTGAAA GAAGCTACCG CGTCGAAATG GCAATCATCA 
TCACCCGCCG CACACACTGA AGGCGAATTG GAGCCTATTG ATAAGTTCGG GAACCCGGTC 
AAGACAAGTC GTTTCCGCTC TCAACGGCTC CATATGGACA GTAAACCATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_683715.1
CDS	1..2391
Translation	MDLLAASVESTLKNLQDKRNFLSEQREHYIDIRSRLVRFINDNDDGEEEGEGQGMVFGDIIISTSKIYLSLGYEYYVEKTKEEAITFVDDKLKLMEDAIEQFNLKIEEAKKTLDNLNHMEDGNGIEEDEANNDEDFLPSMEIREELDDEGNVISSSVTPTTKQPSQSNSKKEQTPAVGPKEKGLAKEKKSKSFEENLKGKLLKRNDEVKKKVQPSKVDTENVYTFADLVQQMDQQDELEDGYIETDEINYDYDAFENSNFKVNDNYEEDDEDEDEEEYLNHSIIPGFEAQSSFLQQIQRLRAQKQSQDHEREEGDVNKSLKPILKKSSFAENSDKKQKKKQVGFASSLEIHEVENLKEENKRQMQSFAVPMYETQESTGIANKMTSDEFDGDLFAKMLGVQEADEVHEKYKEELINQERLEGEASRSNRRTRVSRFRKDRASKKENTLSTFKQETTRSVENEVVEKEPVVGDIIEKEPVVGDVIEKEPVVGDVIEKEPAVTDIVEREPAVNDIVERKPVVGDIIEKEPTINDIVEKEPEINSKSEFETPFKKKKLKSLQKPRSSKSMKKKFDPKILENISDDDYDDDDDGNKKLLSNKSKNNTDEQDKFPSKIQEVSRSMAKTGATVGSEPVRITNVDYHALGGNLDDMVKAYSLGLYDDDLEEDPGTIVEKLEDFKEYNKQVELLRDEIRDFQLENKPVTMEEEENDGNVMNDIIEHEFPESYTNDEDEVALHPGRLQEEVAIEYRRLKEATASKWQSSSPAAHTEGELEPIDKFGNPVKTSRFRSQRLHMDSKP

Target ORF information:

RefSeq Version	NM_001179943.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C Bud27p (BUD27), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001179943.1

ORF Insert Sequence:

ATGGATTTGT TAGCGGCCAG CGTAGAAAGT ACGTTGAAAA ATCTGCAAGA TAAGAGAAAC 
TTTCTTTCGG AACAAAGAGA GCACTATATA GATATCAGAA GCCGACTGGT TAGATTTATC 
AATGACAATG ATGATGGCGA AGAAGAAGGC GAAGGCCAAG GGATGGTGTT TGGTGATATC 
ATAATTTCTA CGAGTAAGAT ATATCTAAGT CTAGGCTATG AGTATTACGT TGAAAAGACC 
AAAGAGGAGG CCATCACGTT TGTTGATGAT AAATTAAAAC TAATGGAGGA CGCCATTGAG 
CAATTTAACC TGAAGATCGA AGAGGCTAAA AAGACCTTAG ACAACTTGAA TCACATGGAG 
GACGGAAATG GAATTGAAGA AGACGAAGCG AACAACGACG AAGATTTTCT GCCCTCTATG 
GAGATTAGAG AAGAACTAGA TGACGAGGGA AATGTCATAA GTAGTTCCGT TACCCCTACA 
ACAAAGCAAC CATCACAATC GAATTCCAAA AAAGAACAGA CACCTGCAGT CGGTCCAAAG 
GAGAAAGGAT TAGCAAAGGA GAAGAAATCA AAGTCATTCG AAGAAAACTT GAAAGGAAAG 
TTATTGAAAA GGAACGATGA AGTAAAGAAA AAAGTGCAGC CATCTAAAGT AGATACAGAA 
AACGTGTATA CATTTGCCGA CTTGGTCCAA CAGATGGACC AACAAGATGA ATTGGAGGAC 
GGCTATATTG AAACTGATGA AATAAATTAT GATTATGATG CGTTCGAAAA CTCCAATTTC 
AAAGTCAATG ATAACTATGA AGAGGATGAC GAGGACGAAG ATGAAGAAGA GTATCTAAAT 
CATTCTATTA TACCTGGCTT TGAAGCGCAG TCCTCATTTT TACAACAAAT TCAACGCCTA 
CGCGCACAAA AGCAATCCCA AGATCATGAG AGGGAAGAAG GTGACGTTAA TAAATCATTA 
AAACCTATTT TGAAAAAGTC TTCTTTTGCT GAGAATTCAG ACAAAAAGCA GAAAAAGAAA 
CAAGTCGGGT TTGCATCTTC TCTAGAAATT CACGAAGTCG AAAACTTGAA AGAAGAGAAC 
AAGAGGCAGA TGCAATCCTT TGCGGTGCCC ATGTATGAAA CCCAGGAAAG CACAGGTATC 
GCTAATAAAA TGACATCGGA TGAGTTTGAT GGTGATTTAT TTGCTAAGAT GTTGGGTGTC 
CAAGAGGCCG ATGAAGTGCA TGAAAAATAT AAAGAGGAAC TGATAAATCA AGAGAGACTT 
GAAGGGGAAG CAAGCAGGTC GAATAGAAGA ACTAGGGTGT CCAGATTCCG GAAGGATAGA 
GCTTCTAAAA AAGAAAATAC GTTATCAACT TTCAAACAGG AAACGACAAG GTCAGTGGAG 
AACGAGGTAG TGGAAAAAGA GCCTGTTGTG GGGGATATTA TAGAAAAAGA ACCTGTTGTG 
GGGGATGTTA TAGAAAAAGA ACCTGTTGTG GGGGATGTTA TAGAAAAAGA GCCTGCTGTC 
ACCGATATAG TGGAAAGGGA GCCTGCTGTC AACGATATAG TGGAAAGGAA GCCTGTTGTG 
GGGGATATTA TAGAAAAAGA ACCTACTATC AACGATATAG TGGAAAAAGA ACCGGAAATT 
AACAGCAAGA GCGAGTTTGA GACTCCTTTT AAAAAGAAAA AGCTAAAATC ACTGCAAAAA 
CCAAGATCAT CGAAATCAAT GAAAAAAAAA TTTGATCCCA AAATTCTCGA AAATATAAGT 
GATGACGATT ATGATGATGA TGACGATGGT AATAAGAAAC TTTTGTCCAA CAAAAGTAAA 
AATAACACTG ATGAGCAAGA TAAATTTCCT TCAAAAATCC AAGAGGTGTC TAGGTCGATG 
GCTAAGACGG GCGCCACAGT AGGGAGTGAG CCGGTTAGAA TTACAAATGT CGATTATCAC 
GCCCTAGGTG GAAACTTGGA TGATATGGTC AAGGCATATT CATTGGGGTT ATATGACGAC 
GATTTAGAGG AAGATCCAGG AACCATAGTG GAAAAGCTCG AGGATTTCAA AGAATACAAT 
AAGCAGGTGG AGTTGCTGCG TGACGAAATT CGAGACTTTC AACTAGAAAA CAAGCCAGTG 
ACGATGGAGG AGGAGGAAAA TGATGGCAAT GTTATGAATG ATATCATTGA GCATGAATTT 
CCTGAAAGTT ACACAAATGA CGAGGACGAA GTAGCACTAC ATCCGGGCCG GCTACAGGAA 
GAGGTTGCTA TAGAGTACAG ACGGTTGAAA GAAGCTACCG CGTCGAAATG GCAATCATCA 
TCACCCGCCG CACACACTGA AGGCGAATTG GAGCCTATTG ATAAGTTCGG GAACCCGGTC 
AAGACAAGTC GTTTCCGCTC TCAACGGCTC CATATGGACA GTAAACCATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

Analysis of the nucleotide sequence of chromosome VI from Saccharomyces cerevisiae.
Nature genetics10(3)261-8(1995 Jul)
Murakami Y,Naitou M,Hagiwara H,Shibata T,Ozawa M,Sasanuma S,Sasanuma M,Tsuchiya Y,Soeda E,Yokoyama K

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BUD27 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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