MIL1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	MIL1
Entrez Gene ID	850510
Full Name	Mil1p
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001179932.2	NP_116583.2	Mil1p

Pathways from BioSystems

Homologies

Saccharomyces cerevisiae (baker's yeast)	YFL034W	NP_116583.2
Kluyveromyces lactis	KLLA0D05159g	XP_453290.1
Eremothecium gossypii	AGOS_ACR220C	NP_983622.1

Gene Ontology
Bibliography

Related articles in PubMed

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

Analysis of the nucleotide sequence of chromosome VI from Saccharomyces cerevisiae.
Murakami Y, Naitou M, Hagiwara H, Shibata T, Ozawa M, Sasanuma S, Sasanuma M, Tsuchiya Y, Soeda E, Yokoyama K
Nature genetics10(3)261-8(1995 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Mil1 is a predicted lipase that binds Apm2 but not Apm1 and contributes to its membrane recruitment.
Title: The alternate AP-1 adaptor subunit Apm2 interacts with the Mil1 regulatory protein and confers differential cargo sorting.

The following MIL1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MIL1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi00203	NM_001179932.2 Latest version!	Saccharomyces cerevisiae S288C Mil1p (MIL1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MIL1 ( NM_001179932.2 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_116583.2 Saccharomyces cerevisiae S288C Mil1p (MIL1), partial mRNA.

Price & Availability↑

CloneID	OSi00203
Clone ID Related Accession (Same CDS sequence)	NM_001179932.2
Accession Version	NM_001179932.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3222bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	Mil1p
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001138). On Apr 26, 2011 this sequence version replaced NM_001179932.1. ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGTCCGATT CGGAGGAAGA TTTAGGTGTA CAGTTGAAAG GCTTGAAGAT AGCGAGGCAT 
TTGAAGGAGT CAGGGGAGCA TACTGATGAA GAATCCAACT CAAGCCCCGA ACATGATTGC 
GGCTTGAGTA ATCAAGATGA TTTAACTGTT ATGCATACAC AGGCTAAAGA AGAAGTTTTT 
AAACGAAGAG AAGAAGACGG AACACGAACC GAAGATGCTT TACATGAGGG AGAAGCAGGA 
AAAGAGGGCA CAGGTTTTCC CTCATCACAG TCAGTGTGCA GTCCTAATGA AGCAGATAGC 
GGTATTGATC GGGCCGATAA ACCTATTTTG CTGGACCCTT TTAAATCTGT GCACGATACG 
GATCCTGTTC CAGGTACAAA GTCCAGGAGC AATTCAGACT CCGACTCTGA TTCAGATGAT 
GGAGGCTGGC AAGAGATGCC CGCTGTCTCA TCGTTCAATA TATATAACCA CAGAGGCGAA 
CTAGAGTTGA CTTCCAAGGT TAGAAACTCA GAACAAGCCT CTGAGACGTC TCCCACAGTT 
CCTCCAGGAA AAAATTGCAA AAGTGTCAAT GATTCTAGAT TTGACTATAC GAAAATGGCC 
GCTGAGCAGC AAGCCCAACG ATCCTATCGA ACAAATAAAA AAACAGATTT CCTTTTTGAC 
CACAAAGTAC TGAAAAAGAA AATTAACAGT TCGCAAACTT CCGTAAATTT GACTTCTTCC 
CCTTCGACTA CATCCTTGAA CAATGAAAAA AATAATGATG ACGATGATGA TGATTCTTAT 
GACGAATATG AGGACGACGT GGAACCAGTG AACGACTTGA ATCGGGACTC GCAGTTAAAC 
ATAACGAAAA ATCTGCTATC TGATATGGAG AAGTTTGCCT ACGTAGGTGC AATAAACATT 
TTGGCAAATC AAATGTGCAC GAATTTGGCT ACGCTGTGTC TTTGTATTGA CATTAAATCC 
CACAAGAAAC TAGCACATAG ATTACAGTTT ACCCAGAAGG ATATGGCTGC TTGGAAAACT 
GTGGTTTTAT CACGGCTATA TGATCATTTG GGGATATCTC AAGAAGAAAT TGTTATGATT 
GAGAAGCTTT CTCTGCACAA AATCCAATTG GAAGATTTGT GTAAATGTTT GAAAACCACT 
CAGAGCATTG ATAATCCATG GGAGAATGAT AGAGACCACG AAGAAGACGG TATAGAGGAA 
ACTACGGAAC GAATGAGTCC AAATGAACAA AATGGCTCAG TACAGGCTAG TACTCCCGAC 
CCAGAACAAT CCGCAACACC TGAAACACCA AAGGCTAAAC AATCACCGTT ATCTTCGGAC 
GTTCCAGGAA AAGTACTCGA TCCGGAAAAT GTCAAAAGCC AAGACAAATT AAATATAGAT 
GTGGCGTGGA CCATTATATG TGATCTTTTC CTAATATGCT TGCAATCCTC CACCTATGAT 
TCGAGGTCAA GGACTTTGTT GATAAATTTT GCCAAAGTCT TGAATATGAC TAGCTTGGAA 
ATTTGTGAAT TTGAAAGACG AGTAACAGAT TCCTTAGACA TGGAACAATC TACAGAAGAT 
CAAGTATGGG ATGAACAAGA TCACATGAGA AATAGACGGA GAAGCAAAAG AAGAAAGAAA 
ATGGCATATG TCGCACTTGC CATGGTAGGT GGTTCTTTGG TCCTTGGACT AAGTGGTGGT 
TTACTAGCCC CCGTCATTGG CGGGGGGATT GCTGCTGGTT TGTCAACAAT AGGTATTACT 
GGTGCTACAA GTTTCTTGAC CGGAGTGGGT GGTACCACTG TTGTTGCCGT TTCAAGTACT 
GCTATTGGTG CCAACATCGG TGCTAGAGGT ATGTCAAAAA GGATGGGAAG TGTGAGAACT 
TTTGAGTTCA GGCCACTGCA TAATAATAGA AGGGTTAATC TAATCCTAAC AGTGTCAGGC 
TGGATGGTTG GTAACGAAGA TGATGTTAGA TTGCCATTTT CTACGGTGGA CCCTGTTGAA 
GGTGACCTGT ACTCGTTATA TTGGGAGCCC GAAATGCTAA AGTCTATTGG TCAAACGGTC 
AGTATTGTAG CTACCGAAAT TTTTACCACC TCGCTTCAAC AAATTTTGGG TGCCACTGTT 
TTAACAGCCT TGATCAGTTC TATCCAATGG CCGATGGCTT TATCGAAATT GGGTTATATT 
TTAGATAATC CGTGGAATGT TTCTTTGGAC AGAGCTTGGT CTGCAGGCAA AATTCTTGCT 
GACACCCTAA TTGCAAGAAA TTTAGGTGCC CGCCCCATTA CACTGGTTGG CTTCTCAATA 
GGTGCGAGGG TCATTTTTTC TTGTTTAATC GAATTGTGCA AGAAAAAGGC TCTAGGTTTG 
ATTGAAAACG TTTATCTATT CGGTACACCA GCTGTCATGA AAAAGGAGCA ACTAGTCATG 
GCAAGATCTG TAGTTAGTGG GAGGTTTGTA AATGGTTATT CGGATAAAGA TTGGTTTTTA 
GCATATTTAT TTAGAGCTGC TGCTGGTGGA TTTAGTGCTG TTATGGGTAT TTCTACGATA 
GAAAACGTTG AAGGTATCGA GAATATTAAT TGCACTGAAT TTGTTGATGG TCATTTGAAT 
TATCGTAAAA GCATGCCTAA GTTATTAAAA AGAATTGGTA TTGCTGTTTT AAGTGAGGAG 
TTTGTCGAGA TAGAGGAGAT GATGAACCCA GAAGAAGTGA AAAGAAAAAG GAAATTAATA 
AATGATGTCG ATGCAGCCCA AAAAAAACTA AGTGAAAGAA AAAAGCATAA CAGTTGGGTG 
CCGAAGTGGT TGAAACCGAA GAAATCCAAA TGGAAGGTCA TGGTCGAAGA AGCTGTCGAA 
GAAGGAAGAG ATATGCAAGA CCTACCAGAA AATGACGTCA ATAACAATGA AAACGAAAAT 
CCAGATGAAC ATGAAGGGAT AGCAAGGCAA AAACGCAGAG ATGCTGCTCT TGTCGATCAT 
GGGGCATTAA TGCATGAGTT ACAACTTATA AAACAAGCGA TGCACGAAGA CGAAATAAAG 
AATAAAGCAT GCCTTCCAGG AGAAGACAAG GAAGTGGAAT CATCAAACGA CTTCTTGGGG 
GAGTCGCATT ACAAACCACC GTCAACACCA AAAATTAATC CACCACAAAG CCCTAATAAC 
TTCCAATTGT TAAGTGCTGG GAGAACTATT CTTCCAGAGG ATGATGATTT CGACCCTCGA 
GGAAAAAAAA AGGTTGAATT CTCTTTCCCA GATGATATCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_116583.2
CDS	1..3222
Translation	MSDSEEDLGVQLKGLKIARHLKESGEHTDEESNSSPEHDCGLSNQDDLTVMHTQAKEEVFKRREEDGTRTEDALHEGEAGKEGTGFPSSQSVCSPNEADSGIDRADKPILLDPFKSVHDTDPVPGTKSRSNSDSDSDSDDGGWQEMPAVSSFNIYNHRGELELTSKVRNSEQASETSPTVPPGKNCKSVNDSRFDYTKMAAEQQAQRSYRTNKKTDFLFDHKVLKKKINSSQTSVNLTSSPSTTSLNNEKNNDDDDDDSYDEYEDDVEPVNDLNRDSQLNITKNLLSDMEKFAYVGAINILANQMCTNLATLCLCIDIKSHKKLAHRLQFTQKDMAAWKTVVLSRLYDHLGISQEEIVMIEKLSLHKIQLEDLCKCLKTTQSIDNPWENDRDHEEDGIEETTERMSPNEQNGSVQASTPDPEQSATPETPKAKQSPLSSDVPGKVLDPENVKSQDKLNIDVAWTIICDLFLICLQSSTYDSRSRTLLINFAKVLNMTSLEICEFERRVTDSLDMEQSTEDQVWDEQDHMRNRRRSKRRKKMAYVALAMVGGSLVLGLSGGLLAPVIGGGIAAGLSTIGITGATSFLTGVGGTTVVAVSSTAIGANIGARGMSKRMGSVRTFEFRPLHNNRRVNLILTVSGWMVGNEDDVRLPFSTVDPVEGDLYSLYWEPEMLKSIGQTVSIVATEIFTTSLQQILGATVLTALISSIQWPMALSKLGYILDNPWNVSLDRAWSAGKILADTLIARNLGARPITLVGFSIGARVIFSCLIELCKKKALGLIENVYLFGTPAVMKKEQLVMARSVVSGRFVNGYSDKDWFLAYLFRAAAGGFSAVMGISTIENVEGIENINCTEFVDGHLNYRKSMPKLLKRIGIAVLSEEFVEIEEMMNPEEVKRKRKLINDVDAAQKKLSERKKHNSWVPKWLKPKKSKWKVMVEEAVEEGRDMQDLPENDVNNNENENPDEHEGIARQKRRDAALVDHGALMHELQLIKQAMHEDEIKNKACLPGEDKEVESSNDFLGESHYKPPSTPKINPPQSPNNFQLLSAGRTILPEDDDFDPRGKKKVEFSFPDDI

Target ORF information:

RefSeq Version	NM_001179932.2
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C Mil1p (MIL1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001179932.2

ORF Insert Sequence:

ATGTCCGATT CGGAGGAAGA TTTAGGTGTA CAGTTGAAAG GCTTGAAGAT AGCGAGGCAT 
TTGAAGGAGT CAGGGGAGCA TACTGATGAA GAATCCAACT CAAGCCCCGA ACATGATTGC 
GGCTTGAGTA ATCAAGATGA TTTAACTGTT ATGCATACAC AGGCTAAAGA AGAAGTTTTT 
AAACGAAGAG AAGAAGACGG AACACGAACC GAAGATGCTT TACATGAGGG AGAAGCAGGA 
AAAGAGGGCA CAGGTTTTCC CTCATCACAG TCAGTGTGCA GTCCTAATGA AGCAGATAGC 
GGTATTGATC GGGCCGATAA ACCTATTTTG CTGGACCCTT TTAAATCTGT GCACGATACG 
GATCCTGTTC CAGGTACAAA GTCCAGGAGC AATTCAGACT CCGACTCTGA TTCAGATGAT 
GGAGGCTGGC AAGAGATGCC CGCTGTCTCA TCGTTCAATA TATATAACCA CAGAGGCGAA 
CTAGAGTTGA CTTCCAAGGT TAGAAACTCA GAACAAGCCT CTGAGACGTC TCCCACAGTT 
CCTCCAGGAA AAAATTGCAA AAGTGTCAAT GATTCTAGAT TTGACTATAC GAAAATGGCC 
GCTGAGCAGC AAGCCCAACG ATCCTATCGA ACAAATAAAA AAACAGATTT CCTTTTTGAC 
CACAAAGTAC TGAAAAAGAA AATTAACAGT TCGCAAACTT CCGTAAATTT GACTTCTTCC 
CCTTCGACTA CATCCTTGAA CAATGAAAAA AATAATGATG ACGATGATGA TGATTCTTAT 
GACGAATATG AGGACGACGT GGAACCAGTG AACGACTTGA ATCGGGACTC GCAGTTAAAC 
ATAACGAAAA ATCTGCTATC TGATATGGAG AAGTTTGCCT ACGTAGGTGC AATAAACATT 
TTGGCAAATC AAATGTGCAC GAATTTGGCT ACGCTGTGTC TTTGTATTGA CATTAAATCC 
CACAAGAAAC TAGCACATAG ATTACAGTTT ACCCAGAAGG ATATGGCTGC TTGGAAAACT 
GTGGTTTTAT CACGGCTATA TGATCATTTG GGGATATCTC AAGAAGAAAT TGTTATGATT 
GAGAAGCTTT CTCTGCACAA AATCCAATTG GAAGATTTGT GTAAATGTTT GAAAACCACT 
CAGAGCATTG ATAATCCATG GGAGAATGAT AGAGACCACG AAGAAGACGG TATAGAGGAA 
ACTACGGAAC GAATGAGTCC AAATGAACAA AATGGCTCAG TACAGGCTAG TACTCCCGAC 
CCAGAACAAT CCGCAACACC TGAAACACCA AAGGCTAAAC AATCACCGTT ATCTTCGGAC 
GTTCCAGGAA AAGTACTCGA TCCGGAAAAT GTCAAAAGCC AAGACAAATT AAATATAGAT 
GTGGCGTGGA CCATTATATG TGATCTTTTC CTAATATGCT TGCAATCCTC CACCTATGAT 
TCGAGGTCAA GGACTTTGTT GATAAATTTT GCCAAAGTCT TGAATATGAC TAGCTTGGAA 
ATTTGTGAAT TTGAAAGACG AGTAACAGAT TCCTTAGACA TGGAACAATC TACAGAAGAT 
CAAGTATGGG ATGAACAAGA TCACATGAGA AATAGACGGA GAAGCAAAAG AAGAAAGAAA 
ATGGCATATG TCGCACTTGC CATGGTAGGT GGTTCTTTGG TCCTTGGACT AAGTGGTGGT 
TTACTAGCCC CCGTCATTGG CGGGGGGATT GCTGCTGGTT TGTCAACAAT AGGTATTACT 
GGTGCTACAA GTTTCTTGAC CGGAGTGGGT GGTACCACTG TTGTTGCCGT TTCAAGTACT 
GCTATTGGTG CCAACATCGG TGCTAGAGGT ATGTCAAAAA GGATGGGAAG TGTGAGAACT 
TTTGAGTTCA GGCCACTGCA TAATAATAGA AGGGTTAATC TAATCCTAAC AGTGTCAGGC 
TGGATGGTTG GTAACGAAGA TGATGTTAGA TTGCCATTTT CTACGGTGGA CCCTGTTGAA 
GGTGACCTGT ACTCGTTATA TTGGGAGCCC GAAATGCTAA AGTCTATTGG TCAAACGGTC 
AGTATTGTAG CTACCGAAAT TTTTACCACC TCGCTTCAAC AAATTTTGGG TGCCACTGTT 
TTAACAGCCT TGATCAGTTC TATCCAATGG CCGATGGCTT TATCGAAATT GGGTTATATT 
TTAGATAATC CGTGGAATGT TTCTTTGGAC AGAGCTTGGT CTGCAGGCAA AATTCTTGCT 
GACACCCTAA TTGCAAGAAA TTTAGGTGCC CGCCCCATTA CACTGGTTGG CTTCTCAATA 
GGTGCGAGGG TCATTTTTTC TTGTTTAATC GAATTGTGCA AGAAAAAGGC TCTAGGTTTG 
ATTGAAAACG TTTATCTATT CGGTACACCA GCTGTCATGA AAAAGGAGCA ACTAGTCATG 
GCAAGATCTG TAGTTAGTGG GAGGTTTGTA AATGGTTATT CGGATAAAGA TTGGTTTTTA 
GCATATTTAT TTAGAGCTGC TGCTGGTGGA TTTAGTGCTG TTATGGGTAT TTCTACGATA 
GAAAACGTTG AAGGTATCGA GAATATTAAT TGCACTGAAT TTGTTGATGG TCATTTGAAT 
TATCGTAAAA GCATGCCTAA GTTATTAAAA AGAATTGGTA TTGCTGTTTT AAGTGAGGAG 
TTTGTCGAGA TAGAGGAGAT GATGAACCCA GAAGAAGTGA AAAGAAAAAG GAAATTAATA 
AATGATGTCG ATGCAGCCCA AAAAAAACTA AGTGAAAGAA AAAAGCATAA CAGTTGGGTG 
CCGAAGTGGT TGAAACCGAA GAAATCCAAA TGGAAGGTCA TGGTCGAAGA AGCTGTCGAA 
GAAGGAAGAG ATATGCAAGA CCTACCAGAA AATGACGTCA ATAACAATGA AAACGAAAAT 
CCAGATGAAC ATGAAGGGAT AGCAAGGCAA AAACGCAGAG ATGCTGCTCT TGTCGATCAT 
GGGGCATTAA TGCATGAGTT ACAACTTATA AAACAAGCGA TGCACGAAGA CGAAATAAAG 
AATAAAGCAT GCCTTCCAGG AGAAGACAAG GAAGTGGAAT CATCAAACGA CTTCTTGGGG 
GAGTCGCATT ACAAACCACC GTCAACACCA AAAATTAATC CACCACAAAG CCCTAATAAC 
TTCCAATTGT TAAGTGCTGG GAGAACTATT CTTCCAGAGG ATGATGATTT CGACCCTCGA 
GGAAAAAAAA AGGTTGAATT CTCTTTCCCA GATGATATCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

Analysis of the nucleotide sequence of chromosome VI from Saccharomyces cerevisiae.
Nature genetics10(3)261-8(1995 Jul)
Murakami Y,Naitou M,Hagiwara H,Shibata T,Ozawa M,Sasanuma S,Sasanuma M,Tsuchiya Y,Soeda E,Yokoyama K

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MIL1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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