RPO41 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	RPO41
Entrez Gene ID	850507
Full Name	DNA-directed RNA polymerase
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001179930.1	NP_116617.1	DNA-directed RNA polymerase

Pathways from BioSystems

Homologies

Schizosaccharomyces pombe (fission yeast)	rpo41	NP_594459.2
Saccharomyces cerevisiae (baker's yeast)	RPO41	NP_116617.1
Eremothecium gossypii	AGOS_ACR224C	NP_983626.1
Neurospora crassa	NCU06308	XP_962570.2
Oryza sativa (rice)	Os09g0246200	NP_001062659.1
Kluyveromyces lactis	KLLA0D05247g	XP_453294.1
Magnaporthe oryzae (rice blast fungus)	MGG_14133	XP_003719210.1
Arabidopsis thaliana (thale cress)	AT5G15700	NP_197074.1

Gene Ontology
Bibliography

Related articles in PubMed

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

Analysis of the nucleotide sequence of chromosome VI from Saccharomyces cerevisiae.
Murakami Y, Naitou M, Hagiwara H, Shibata T, Ozawa M, Sasanuma S, Sasanuma M, Tsuchiya Y, Soeda E, Yokoyama K
Nature genetics10(3)261-8(1995 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

results thus indicate that the PPR motifs do not play an essential role in the function of the NTE domain of the mitochondrial RNA polymerase Rpo41p.
Title: Pentatricopeptide Motifs in the N-Terminal Extension Domain of Yeast Mitochondrial RNA Polymerase Rpo41p Are Not Essential for Its Function.

Rpo41-Mtf1 is an attractive candidate for serving as the primase to initiate lagging strand DNA synthesis during normal replication and/or to restart stalled replication from downstream ssDNA.
Title: The Yeast Mitochondrial RNA Polymerase and Transcription Factor Complex Catalyzes Efficient Priming of DNA Synthesis on Single-stranded DNA.

Herein the authors found that Rpo41 transcribes RNAs that can be extended by Mip1 on single and double-stranded DNA.
Title: Yeast mitochondrial RNA polymerase primes mitochondrial DNA polymerase at origins of replication and promoter sequences.

The study examined a series of N-terminal deletion mutants of yeast mitochondrial RNA polymerase, Rpo41, and found that the N-terminal region does not abolish the effects of Mtf1; rather it contributes directly to enzyme catalysis.
Title: Yeast Mitochondrial Transcription Factor Mtf1 Determines the Precision of Promoter-Directed Initiation of RNA Polymerase Rpo41.

This study identifies the importance of the +1+2 AA promoter sequence interactions with Rpo41-Mtf1 in dictating pre-transcribing complex formation, which regulates the binding of initiating NTPs and the catalytic efficiency of transcription initiation.
Title: Interactions of the yeast mitochondrial RNA polymerase with the +1 and +2 promoter bases dictate transcription initiation efficiency.

The following RPO41 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RPO41 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi00200	NM_001179930.1 Latest version!	Saccharomyces cerevisiae S288C DNA-directed RNA polymerase (RPO41), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RPO41 ( NM_001179930.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_116617.1 Saccharomyces cerevisiae S288C DNA-directed RNA polymerase (RPO41), partial mRNA.

Price & Availability↑

CloneID	OSi00200
Clone ID Related Accession (Same CDS sequence)	NM_001179930.1
Accession Version	NM_001179930.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4056bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	DNA-directed RNA polymerase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001138). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGCTGAGAC CGGCCTATAA ATCGCTCGTG AAAACATCCC TGTTACAAAG ACGGTTAATA 
TCTTCAAAGG GATCTAAGCT GTTTAAACCA TCTCCGGACT CAACAAGTAC AATATTAATA 
TCTGAAGACC CCCTTGTAAC AGGCTCGTCC CCAACCTCAT CTACAACATC AGGTATAATA 
AGTTCAAATG ATTTCCCTTT GTTCAACAAA AATAGGAAAG ACGCCAAGAG CAGCATATCC 
TACCAATGGA AAAATCCTTC AGAATTAGAG TTTGACCCTT TCAATAAATC GCATGCCTCA 
GCAGTAACCT CGATGACAAG AACTAGAGAC GTTATGCAAT TATGGTCTCT GTTAGAAGCA 
TGCTTGCAAT CGAATCTAAT GAAAAGAGCG TTCTCTATAT TGGAGTCACT GTACCTGGTA 
CCTGAACATA AGCAGCGATT TATCGAGGAT TATAACATGT ACTTGAATTC ATTTTCCAAA 
AATGACCCTA ATTTCCCTAT TTTAAAAATG AACGAAAAAT TAACAAATGA TTTGGAGACA 
AGTTTTAAAG ACGTTAATTA TAATGACAAA ACTTTGGCAA TAATGATTCA TCACGCTTTG 
AACTTCCATT CAACGACAAG CTCGATGCTT TTGAAGCCTA TAATCTCTGC CTACTTGAAA 
ATGAGCGTGA ATGGTATTAG GGAAATTTTC TCCTGCCTAG ATATTTTGAC CATTTCAGAT 
CTGAATATTT TAATGAATGA CTTAAAGGTT ATAACACCAT CGCAACTGCC AAATTCTGTA 
AGACCAATAT TAGAATCACT TACGCTATCT CCTACACCTG TAAATAATAT TGAAAATGAG 
GAGGGTTTGA ACAAAGTGGA AGCTGAGAAC GATTCAAAAT TGCATAAAGC ATCAAATGCA 
AGCTCTGATT CTATAAAAAA ACCTTCCTTG GATCCTCTTC GGGAAGTTTC ATTCCACGGT 
TCAACAGAAG TCTTGTCAAA AGACGCTGAA AAGCTTATAG CTGTAGACAC TATTGGAATG 
AGAGTAATCC GGCATACTTT GCTGGGATTA TCATTAACGC CGGAACAGAA AGAGCAAATT 
TCTAAATTCA AATTTGATGC CAATGATAAT GTTTTGAAAA TGAAACCTAC GAAAAACGAC 
GATAATAATA ATTCTATAAA TTTTTTTGAA ATTTACAATT CACTGCCAAC ATTAGAGGAA 
AAGAAGGCAT TCGAAAGCGC ATTAAATATA TTTAACCAAG ATAGACAAAA GGTACTTGAA 
AACCGTGCAA CGGAAGCCGC AAGAGAGCGT TGGAAGCACG ATTTTGAAGA GGCAAAAGCT 
AGAGGTGACA TTTCAATTGA AAAGAATTTA AACGTTAAGT TGTGGAAATG GTATAATGAA 
ATGTTACCTC TAGTGAAGGA AGAAATTAAT CATTGTAGGT CATTATTGTC AGAAAAGCTA 
TCAGATAAGA AAGGCTTGAA TAAAGTTGAC ACGAACCGTC TTGGATATGG ACCATACTTA 
ACCTTGATTG ACCCTGGTAA AATGTGTGTC ATCACCATTT TGGAACTATT AAAGTTAAAT 
TCTACGGGTG GTGTTATTGA AGGTATGAGG ACCGCAAGAG CGGTTATATC AGTGGGTAAA 
GCTATTGAAA TGGAATTTAG ATCCGAGCAG GTTTTGAAAA GCGAATCTCA AGCGTTCAGA 
GATGTTAACA AGAAATCACC AGAATTCAAA AAACTGGTAC AAAATGCTAA ATCTGTATTC 
AGATCATCTC AAATTGAGCA GTCGAAAATT TTGTGGCCTC AGAGTATTAG GGCCAGAATC 
GGTTCGGTAT TAATCTCAAT GTTGATTCAA GTCGCTAAGG TATCTGTGCA AGGTGTTGAC 
CCTGTGACAA AAGCTAAGGT TCACGGTGAA GCTCCAGCTT TTGCGCATGG TTATCAGTAC 
CACAATGGTT CCAAATTGGG TGTCTTGAAG ATTCACAAGA CTTTGATTCG TCAATTGAAC 
GGTGAAAGAT TGATTGCCTC TGTCCAACCA CAATTATTAC CAATGTTGGT GGAACCCAAA 
CCCTGGGTTA ATTGGAGATC AGGTGGATAT CATTATACAC AATCTACCCT TTTAAGAACA 
AAAGACTCAC CCGAACAAGT AGCATATTTG AAAGCTGCTT CCGATAATGG TGATATAGAC 
CGCGTTTATG ATGGTTTGAA TGTTCTGGGT AGAACACCGT GGACTGTCAA CAGAAAAGTT 
TTTGATGTGG TTTCACAAGT TTGGAATAAA GGTGAAGGTT TCCTAGATAT TCCAGGTGCA 
CAAGACGAAA TGGTTTTACC ACCAGCTCCT CCGAAGAACT CCGACCCTTC CATTTTGAGA 
GCCTGGAAAC TTCAAGTCAA AACAATTGCT AATAAATTCT CTTCAGATAG ATCAAATAGA 
TGTGATACGA ACTACAAATT GGAAATTGCA AGAGCATTTT TGGGTGAAAA GCTGTACTTT 
CCACATAATT TGGACTTTAG GGGACGTGCT TATCCTTTGT CTCCACATTT CAATCATCTT 
GGTAATGATA TGAGTCGTGG CCTTTTGATT TTTTGGCACG GTAAAAAATT AGGACCTTCT 
GGTCTCAAGT GGTTAAAGAT TCATTTGTCA AATCTGTTCG GATTTGATAA GTTACCCCTA 
AAGGATCGTG TTGCATTTAC TGAGTCTCAT TTGCAGGACA TCAAGGATTC TGCGGAAAAT 
CCCTTAACGG GAGATCGTTG GTGGACTACT GCTGATAAGC CATGGCAAGC ATTGGCCACA 
TGCTTTGAAT TGAATGAAGT TATGAAAATG GATAATCCAG AGGAGTTTAT CTCTCATCAA 
CCGGTTCACC AAGACGGTAC TTGCAATGGT TTGCAACATT ATGCTGCCTT GGGTGGTGAC 
GTTGAAGGGG CTACTCAAGT GAATCTGGTT CCAAGCGATA AACCACAAGA TGTTTATGCT 
CATGTTGCAA GGTTGGTGCA AAAAAGATTA GAAATTGCAG CAGAAAAAGG AGACGAAAAC 
GCCAAAATTT TGAAGGATAA GATTACCAGA AAGGTTGTCA AGCAAACCGT CATGACAAAC 
GTTTATGGTG TTACCTACGT CGGGGCTACT TTTCAAATTG CTAAACAATT AAGCCCAATA 
TTTGATGATC GTAAAGAAAG CTTAGACTTT TCTAAATACC TAACCAAACA TGTTTTTAGT 
GCTATCCGCG AGTTATTTCA TAGTGCGCAT TTAATTCAGG ATTGGTTAGG TGAAAGTGCG 
AAAAGAATTT CCAAATCTAT CAGGTTGGAT GTTGATGAAA AATCGTTCAA GAACGGTAAT 
AAACCCGACT TTATGTCATC CGTCATATGG ACGACACCAC TAGGACTACC AATTGTTCAG 
CCATATCGTG AAGAAAGCAA AAAGCAAGTT GAGACCAATT TGCAAACTGT CTTTATTAGC 
GATCCTTTCG CTGTCAACCC TGTTAATGCA AGAAGGCAAA AGGCAGGTCT TCCACCGAAC 
TTCATCCATT CGCTGGATGC TTCACATATG TTGCTAAGTG CAGCAGAATG TGGAAAGCAA 
GGATTAGATT TCGCATCAGT TCATGATTCT TACTGGACGC ATGCATCTGA TATAGACACT 
ATGAATGTGG TGTTAAGGGA ACAGTTTATC AAACTCCATG AGGTAGATCT GGTTTTAAGA 
TTGAAGGAGG AGTTTGACCA AAGGTATAAG AATTACGTCA AGATTGGAAA GTTGAAGAGA 
TCTACGGATT TGGCTCAAAA GATTATTAGA ATCCGTAAAG ATTTATCCAG AAAATTAGGA 
AGAAGTACTA CATTAGCAGA TGAAATTTAT TTTGAGAAAA AAAGGCAAGA ATTATTAAAC 
AGCCCTCTAA TAGAAGATAG AAATGTTGGT GAAAAGATGG TTACAACTGT ATCATTATTT 
GAAGACATCA CTGACTTGGA CGCACTTGAA CTAGAAAACG GTGGAGATGA AAATAGCGGT 
ATGAGTGTTC TTTTACCGTT GAGATTACCT GAAATCCCAC CTAAAGGTGA TTTTGATGTT 
ACAGTATTGA GAAACAGTCA ATATTTTTTC TCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_116617.1
CDS	1..4056
Translation	MLRPAYKSLVKTSLLQRRLISSKGSKLFKPSPDSTSTILISEDPLVTGSSPTSSTTSGIISSNDFPLFNKNRKDAKSSISYQWKNPSELEFDPFNKSHASAVTSMTRTRDVMQLWSLLEACLQSNLMKRAFSILESLYLVPEHKQRFIEDYNMYLNSFSKNDPNFPILKMNEKLTNDLETSFKDVNYNDKTLAIMIHHALNFHSTTSSMLLKPIISAYLKMSVNGIREIFSCLDILTISDLNILMNDLKVITPSQLPNSVRPILESLTLSPTPVNNIENEEGLNKVEAENDSKLHKASNASSDSIKKPSLDPLREVSFHGSTEVLSKDAEKLIAVDTIGMRVIRHTLLGLSLTPEQKEQISKFKFDANDNVLKMKPTKNDDNNNSINFFEIYNSLPTLEEKKAFESALNIFNQDRQKVLENRATEAARERWKHDFEEAKARGDISIEKNLNVKLWKWYNEMLPLVKEEINHCRSLLSEKLSDKKGLNKVDTNRLGYGPYLTLIDPGKMCVITILELLKLNSTGGVIEGMRTARAVISVGKAIEMEFRSEQVLKSESQAFRDVNKKSPEFKKLVQNAKSVFRSSQIEQSKILWPQSIRARIGSVLISMLIQVAKVSVQGVDPVTKAKVHGEAPAFAHGYQYHNGSKLGVLKIHKTLIRQLNGERLIASVQPQLLPMLVEPKPWVNWRSGGYHYTQSTLLRTKDSPEQVAYLKAASDNGDIDRVYDGLNVLGRTPWTVNRKVFDVVSQVWNKGEGFLDIPGAQDEMVLPPAPPKNSDPSILRAWKLQVKTIANKFSSDRSNRCDTNYKLEIARAFLGEKLYFPHNLDFRGRAYPLSPHFNHLGNDMSRGLLIFWHGKKLGPSGLKWLKIHLSNLFGFDKLPLKDRVAFTESHLQDIKDSAENPLTGDRWWTTADKPWQALATCFELNEVMKMDNPEEFISHQPVHQDGTCNGLQHYAALGGDVEGATQVNLVPSDKPQDVYAHVARLVQKRLEIAAEKGDENAKILKDKITRKVVKQTVMTNVYGVTYVGATFQIAKQLSPIFDDRKESLDFSKYLTKHVFSAIRELFHSAHLIQDWLGESAKRISKSIRLDVDEKSFKNGNKPDFMSSVIWTTPLGLPIVQPYREESKKQVETNLQTVFISDPFAVNPVNARRQKAGLPPNFIHSLDASHMLLSAAECGKQGLDFASVHDSYWTHASDIDTMNVVLREQFIKLHEVDLVLRLKEEFDQRYKNYVKIGKLKRSTDLAQKIIRIRKDLSRKLGRSTTLADEIYFEKKRQELLNSPLIEDRNVGEKMVTTVSLFEDITDLDALELENGGDENSGMSVLLPLRLPEIPPKGDFDVTVLRNSQYFFS

Target ORF information:

RefSeq Version	NM_001179930.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C DNA-directed RNA polymerase (RPO41), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001179930.1

ORF Insert Sequence:

ATGCTGAGAC CGGCCTATAA ATCGCTCGTG AAAACATCCC TGTTACAAAG ACGGTTAATA 
TCTTCAAAGG GATCTAAGCT GTTTAAACCA TCTCCGGACT CAACAAGTAC AATATTAATA 
TCTGAAGACC CCCTTGTAAC AGGCTCGTCC CCAACCTCAT CTACAACATC AGGTATAATA 
AGTTCAAATG ATTTCCCTTT GTTCAACAAA AATAGGAAAG ACGCCAAGAG CAGCATATCC 
TACCAATGGA AAAATCCTTC AGAATTAGAG TTTGACCCTT TCAATAAATC GCATGCCTCA 
GCAGTAACCT CGATGACAAG AACTAGAGAC GTTATGCAAT TATGGTCTCT GTTAGAAGCA 
TGCTTGCAAT CGAATCTAAT GAAAAGAGCG TTCTCTATAT TGGAGTCACT GTACCTGGTA 
CCTGAACATA AGCAGCGATT TATCGAGGAT TATAACATGT ACTTGAATTC ATTTTCCAAA 
AATGACCCTA ATTTCCCTAT TTTAAAAATG AACGAAAAAT TAACAAATGA TTTGGAGACA 
AGTTTTAAAG ACGTTAATTA TAATGACAAA ACTTTGGCAA TAATGATTCA TCACGCTTTG 
AACTTCCATT CAACGACAAG CTCGATGCTT TTGAAGCCTA TAATCTCTGC CTACTTGAAA 
ATGAGCGTGA ATGGTATTAG GGAAATTTTC TCCTGCCTAG ATATTTTGAC CATTTCAGAT 
CTGAATATTT TAATGAATGA CTTAAAGGTT ATAACACCAT CGCAACTGCC AAATTCTGTA 
AGACCAATAT TAGAATCACT TACGCTATCT CCTACACCTG TAAATAATAT TGAAAATGAG 
GAGGGTTTGA ACAAAGTGGA AGCTGAGAAC GATTCAAAAT TGCATAAAGC ATCAAATGCA 
AGCTCTGATT CTATAAAAAA ACCTTCCTTG GATCCTCTTC GGGAAGTTTC ATTCCACGGT 
TCAACAGAAG TCTTGTCAAA AGACGCTGAA AAGCTTATAG CTGTAGACAC TATTGGAATG 
AGAGTAATCC GGCATACTTT GCTGGGATTA TCATTAACGC CGGAACAGAA AGAGCAAATT 
TCTAAATTCA AATTTGATGC CAATGATAAT GTTTTGAAAA TGAAACCTAC GAAAAACGAC 
GATAATAATA ATTCTATAAA TTTTTTTGAA ATTTACAATT CACTGCCAAC ATTAGAGGAA 
AAGAAGGCAT TCGAAAGCGC ATTAAATATA TTTAACCAAG ATAGACAAAA GGTACTTGAA 
AACCGTGCAA CGGAAGCCGC AAGAGAGCGT TGGAAGCACG ATTTTGAAGA GGCAAAAGCT 
AGAGGTGACA TTTCAATTGA AAAGAATTTA AACGTTAAGT TGTGGAAATG GTATAATGAA 
ATGTTACCTC TAGTGAAGGA AGAAATTAAT CATTGTAGGT CATTATTGTC AGAAAAGCTA 
TCAGATAAGA AAGGCTTGAA TAAAGTTGAC ACGAACCGTC TTGGATATGG ACCATACTTA 
ACCTTGATTG ACCCTGGTAA AATGTGTGTC ATCACCATTT TGGAACTATT AAAGTTAAAT 
TCTACGGGTG GTGTTATTGA AGGTATGAGG ACCGCAAGAG CGGTTATATC AGTGGGTAAA 
GCTATTGAAA TGGAATTTAG ATCCGAGCAG GTTTTGAAAA GCGAATCTCA AGCGTTCAGA 
GATGTTAACA AGAAATCACC AGAATTCAAA AAACTGGTAC AAAATGCTAA ATCTGTATTC 
AGATCATCTC AAATTGAGCA GTCGAAAATT TTGTGGCCTC AGAGTATTAG GGCCAGAATC 
GGTTCGGTAT TAATCTCAAT GTTGATTCAA GTCGCTAAGG TATCTGTGCA AGGTGTTGAC 
CCTGTGACAA AAGCTAAGGT TCACGGTGAA GCTCCAGCTT TTGCGCATGG TTATCAGTAC 
CACAATGGTT CCAAATTGGG TGTCTTGAAG ATTCACAAGA CTTTGATTCG TCAATTGAAC 
GGTGAAAGAT TGATTGCCTC TGTCCAACCA CAATTATTAC CAATGTTGGT GGAACCCAAA 
CCCTGGGTTA ATTGGAGATC AGGTGGATAT CATTATACAC AATCTACCCT TTTAAGAACA 
AAAGACTCAC CCGAACAAGT AGCATATTTG AAAGCTGCTT CCGATAATGG TGATATAGAC 
CGCGTTTATG ATGGTTTGAA TGTTCTGGGT AGAACACCGT GGACTGTCAA CAGAAAAGTT 
TTTGATGTGG TTTCACAAGT TTGGAATAAA GGTGAAGGTT TCCTAGATAT TCCAGGTGCA 
CAAGACGAAA TGGTTTTACC ACCAGCTCCT CCGAAGAACT CCGACCCTTC CATTTTGAGA 
GCCTGGAAAC TTCAAGTCAA AACAATTGCT AATAAATTCT CTTCAGATAG ATCAAATAGA 
TGTGATACGA ACTACAAATT GGAAATTGCA AGAGCATTTT TGGGTGAAAA GCTGTACTTT 
CCACATAATT TGGACTTTAG GGGACGTGCT TATCCTTTGT CTCCACATTT CAATCATCTT 
GGTAATGATA TGAGTCGTGG CCTTTTGATT TTTTGGCACG GTAAAAAATT AGGACCTTCT 
GGTCTCAAGT GGTTAAAGAT TCATTTGTCA AATCTGTTCG GATTTGATAA GTTACCCCTA 
AAGGATCGTG TTGCATTTAC TGAGTCTCAT TTGCAGGACA TCAAGGATTC TGCGGAAAAT 
CCCTTAACGG GAGATCGTTG GTGGACTACT GCTGATAAGC CATGGCAAGC ATTGGCCACA 
TGCTTTGAAT TGAATGAAGT TATGAAAATG GATAATCCAG AGGAGTTTAT CTCTCATCAA 
CCGGTTCACC AAGACGGTAC TTGCAATGGT TTGCAACATT ATGCTGCCTT GGGTGGTGAC 
GTTGAAGGGG CTACTCAAGT GAATCTGGTT CCAAGCGATA AACCACAAGA TGTTTATGCT 
CATGTTGCAA GGTTGGTGCA AAAAAGATTA GAAATTGCAG CAGAAAAAGG AGACGAAAAC 
GCCAAAATTT TGAAGGATAA GATTACCAGA AAGGTTGTCA AGCAAACCGT CATGACAAAC 
GTTTATGGTG TTACCTACGT CGGGGCTACT TTTCAAATTG CTAAACAATT AAGCCCAATA 
TTTGATGATC GTAAAGAAAG CTTAGACTTT TCTAAATACC TAACCAAACA TGTTTTTAGT 
GCTATCCGCG AGTTATTTCA TAGTGCGCAT TTAATTCAGG ATTGGTTAGG TGAAAGTGCG 
AAAAGAATTT CCAAATCTAT CAGGTTGGAT GTTGATGAAA AATCGTTCAA GAACGGTAAT 
AAACCCGACT TTATGTCATC CGTCATATGG ACGACACCAC TAGGACTACC AATTGTTCAG 
CCATATCGTG AAGAAAGCAA AAAGCAAGTT GAGACCAATT TGCAAACTGT CTTTATTAGC 
GATCCTTTCG CTGTCAACCC TGTTAATGCA AGAAGGCAAA AGGCAGGTCT TCCACCGAAC 
TTCATCCATT CGCTGGATGC TTCACATATG TTGCTAAGTG CAGCAGAATG TGGAAAGCAA 
GGATTAGATT TCGCATCAGT TCATGATTCT TACTGGACGC ATGCATCTGA TATAGACACT 
ATGAATGTGG TGTTAAGGGA ACAGTTTATC AAACTCCATG AGGTAGATCT GGTTTTAAGA 
TTGAAGGAGG AGTTTGACCA AAGGTATAAG AATTACGTCA AGATTGGAAA GTTGAAGAGA 
TCTACGGATT TGGCTCAAAA GATTATTAGA ATCCGTAAAG ATTTATCCAG AAAATTAGGA 
AGAAGTACTA CATTAGCAGA TGAAATTTAT TTTGAGAAAA AAAGGCAAGA ATTATTAAAC 
AGCCCTCTAA TAGAAGATAG AAATGTTGGT GAAAAGATGG TTACAACTGT ATCATTATTT 
GAAGACATCA CTGACTTGGA CGCACTTGAA CTAGAAAACG GTGGAGATGA AAATAGCGGT 
ATGAGTGTTC TTTTACCGTT GAGATTACCT GAAATCCCAC CTAAAGGTGA TTTTGATGTT 
ACAGTATTGA GAAACAGTCA ATATTTTTTC TCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

Analysis of the nucleotide sequence of chromosome VI from Saccharomyces cerevisiae.
Nature genetics10(3)261-8(1995 Jul)
Murakami Y,Naitou M,Hagiwara H,Shibata T,Ozawa M,Sasanuma S,Sasanuma M,Tsuchiya Y,Soeda E,Yokoyama K

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RPO41 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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