YPT1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	YPT1
Entrez Gene ID	850505
Full Name	Rab family GTPase YPT1
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001179928.1	NP_116615.1	Rab family GTPase YPT1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	RAB1A	XP_530454.3
Danio rerio (zebrafish)	rab1a	NP_001007162.1
Saccharomyces cerevisiae (baker's yeast)	YPT1	NP_116615.1
Kluyveromyces lactis	KLLA0D05313g	XP_453297.1
Neurospora crassa	NCU08477	XP_963146.2
Xenopus tropicalis (tropical clawed frog)	rab1a	NP_001004787.1
Caenorhabditis elegans (roundworm)	rab-1	NP_503397.1
Eremothecium gossypii	AGOS_ABR220W	NP_983169.1
Arabidopsis thaliana (thale cress)	RA-5	NP_171715.1
Oryza sativa (rice)	Os01g0558600	NP_001043336.1
Homo sapiens (human)	RAB1A	NP_004152.1
Gallus gallus (chicken)	RAB1A	NP_001257591.1
Drosophila melanogaster (fruit fly)	Rab1	NP_732610.1
Schizosaccharomyces pombe (fission yeast)	ypt1	NP_596205.1
	RAB1A	XP_001090072.1
Canis lupus familiaris (dog)	RAB1A	NP_001003153.1
Magnaporthe oryzae (rice blast fungus)	MGG_06962	XP_003709708.1
Mus musculus (house mouse)	Rab1	NP_033022.1
Rattus norvegicus (Norway rat)	Rab1a	NP_112352.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004146	XP_313029.4

Gene Ontology
Bibliography

Related articles in PubMed

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

Analysis of the nucleotide sequence of chromosome VI from Saccharomyces cerevisiae.
Murakami Y, Naitou M, Hagiwara H, Shibata T, Ozawa M, Sasanuma S, Sasanuma M, Tsuchiya Y, Soeda E, Yokoyama K
Nature genetics10(3)261-8(1995 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Since these there proteinases belong to the CPY pathway, YPT1 is even believed to up-regulate this trafficking pathway in yeast cells. Future studies, however, should be carried out to discover the mechanisms that allow YPT1 to recruit these proteins into yeast vacuoles
Title: Effect of GTP-binding protein (YPT1 protein) on the enhanced yeast vacuolar activity.

that changes in Ypt1 and Ypt31 activity affect Golgi cisternal progression, early-to-transitional and transitional-to-late, respectively
Title: Regulation of Golgi Cisternal Progression by Ypt/Rab GTPases.

results suggest that Ypt1p is involved in the cellular protection process under heat stress conditions
Title: Stress-driven structural and functional switching of Ypt1p from a GTPase to a molecular chaperone mediates thermo tolerance in Saccharomyces cerevisiae.

Ypt1 binds and activates Hrr25 to spatially regulate its kinase activity.
Title: Ypt1/Rab1 regulates Hrr25/CK1? kinase activity in ER-Golgi traffic and macroautophagy.

Finally, three sequential steps of this pathway are delineated: Atg9-dependent exit from the ER en route to autophagy, Ypt1- and core Atgs-mediated pre-autophagsomal-structure organization, and Ypt51-mediated delivery of APs to the vacuole
Title: A Role for Macro-ER-Phagy in ER Quality Control.

The following YPT1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the YPT1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi00198	NM_001179928.1 Latest version!	Saccharomyces cerevisiae S288C Rab family GTPase YPT1 (YPT1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

YPT1 ( NM_001179928.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_116615.1 Saccharomyces cerevisiae S288C Rab family GTPase YPT1 (YPT1), partial mRNA.

Price & Availability↑

CloneID	OSi00198
Clone ID Related Accession (Same CDS sequence)	NM_001179928.1
Accession Version	NM_001179928.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 621bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	Rab family GTPase YPT1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001138). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGAATAGCG AGTACGATTA CCTGTTCAAA CTGCTGTTGA TCGGGAATTC CGGTGTCGGG 
AAGTCCTGTT TACTTTTGAG GTTTTCGGAC GACACATATA CCAACGACTA CATCTCCACA 
ATTGGAGTGG ACTTCAAGAT TAAGACTGTA GAACTGGACG GCAAGACTGT AAAGCTACAG 
ATTTGGGACA CTGCAGGTCA AGAACGTTTC CGTACTATCA CTTCATCTTA CTACCGTGGT 
TCGCATGGGA TCATTATCGT GTATGATGTC ACTGACCAAG AATCCTTCAA CGGCGTGAAG 
ATGTGGCTGC AAGAGATTGA TCGGTATGCA ACCTCAACAG TGTTGAAGCT ATTGGTAGGT 
AACAAGTGTG ATTTAAAGGA CAAGCGTGTC GTGGAATATG ACGTGGCAAA GGAATTTGCG 
GACGCGAATA AGATGCCGTT CTTAGAGACT AGTGCTTTGG ACTCCACCAA CGTCGAGGAT 
GCGTTTTTAA CCATGGCTAG ACAAATCAAG GAAAGTATGT CCCAACAAAA CCTGAACGAA 
ACCACTCAGA AGAAGGAAGA CAAAGGGAAC GTCAACCTGA AGGGACAGAG TTTAACCAAC 
ACCGGTGGGG GCTGCTGTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_116615.1
CDS	1..621
Translation	MNSEYDYLFKLLLIGNSGVGKSCLLLRFSDDTYTNDYISTIGVDFKIKTVELDGKTVKLQIWDTAGQERFRTITSSYYRGSHGIIIVYDVTDQESFNGVKMWLQEIDRYATSTVLKLLVGNKCDLKDKRVVEYDVAKEFADANKMPFLETSALDSTNVEDAFLTMARQIKESMSQQNLNETTQKKEDKGNVNLKGQSLTNTGGGCC

Target ORF information:

RefSeq Version	NM_001179928.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C Rab family GTPase YPT1 (YPT1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001179928.1

ORF Insert Sequence:

ATGAATAGCG AGTACGATTA CCTGTTCAAA CTGCTGTTGA TCGGGAATTC CGGTGTCGGG 
AAGTCCTGTT TACTTTTGAG GTTTTCGGAC GACACATATA CCAACGACTA CATCTCCACA 
ATTGGAGTGG ACTTCAAGAT TAAGACTGTA GAACTGGACG GCAAGACTGT AAAGCTACAG 
ATTTGGGACA CTGCAGGTCA AGAACGTTTC CGTACTATCA CTTCATCTTA CTACCGTGGT 
TCGCATGGGA TCATTATCGT GTATGATGTC ACTGACCAAG AATCCTTCAA CGGCGTGAAG 
ATGTGGCTGC AAGAGATTGA TCGGTATGCA ACCTCAACAG TGTTGAAGCT ATTGGTAGGT 
AACAAGTGTG ATTTAAAGGA CAAGCGTGTC GTGGAATATG ACGTGGCAAA GGAATTTGCG 
GACGCGAATA AGATGCCGTT CTTAGAGACT AGTGCTTTGG ACTCCACCAA CGTCGAGGAT 
GCGTTTTTAA CCATGGCTAG ACAAATCAAG GAAAGTATGT CCCAACAAAA CCTGAACGAA 
ACCACTCAGA AGAAGGAAGA CAAAGGGAAC GTCAACCTGA AGGGACAGAG TTTAACCAAC 
ACCGGTGGGG GCTGCTGTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

Analysis of the nucleotide sequence of chromosome VI from Saccharomyces cerevisiae.
Nature genetics10(3)261-8(1995 Jul)
Murakami Y,Naitou M,Hagiwara H,Shibata T,Ozawa M,Sasanuma S,Sasanuma M,Tsuchiya Y,Soeda E,Yokoyama K

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YPT1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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