ORF » Species Summary » Saccharomyces cerevisiae S288C » BUD5 cDNA ORF clone

BUD5 cDNA ORF clone, Saccharomyces cerevisiae S288C

  • Gene

  • Clones

  • gRNAs

The following BUD5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the BUD5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OSi00114 NM_001178752.1
Latest version!		 Saccharomyces cerevisiae S288C Ras family guanine nucleotide exchange factor BUD5 (BUD5), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    BUD5 ( NM_001178752.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_009967.2 Saccharomyces cerevisiae S288C Ras family guanine nucleotide exchange factor BUD5 (BUD5), partial mRNA.
    Price & Availability↑
    CloneID OSi00114
    Clone ID Related Accession (Same CDS sequence) NM_001178752.1
    Accession Version NM_001178752.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1929bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-31
    Organism Saccharomyces cerevisiae S288C
    Product Ras family guanine nucleotide exchange factor BUD5
    Comment Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001135). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGAGCCCGA AAAACAAATA TGTATATATC TGTGTAGAAT ATATATATAT ATATTTCGCA 
    AAAATACATA AACAATCAAC CCTCTCCTCA GACACTACTA AGATGTTTGT ACTGATAGAT 
    AATGTCCTCG CCTACCTTCT TGAACAAGAC GACCTATTTG TAACCGCACG CTTCGCTATC 
    CAGGGCCAAA TCGTCTCAAG AAGAGTTAAC AAAATACATA TCTCCAACAT CACAGACGTC 
    CTTCTACAAC AATTCATAAG TCACACACTG CCCTATAATG ACAATATCGT CCCAAAAAAA 
    ATCCTCGACA GCATGAGAAC GGCCGTACGG CAGTTGCTGG AAGCAACTGC CTGTGTCTCT 
    AGAGAATGCC CCCTCGTCAA AAGAAGTCAG GACATAAAAA GAGCAAGAAA ACGTCTACTC 
    AGTGACTGGT ATAGGCTCGG CGCTGATGCA AACATGGATG CCGTATTACT AGTTGTTAAC 
    TCCGCCTGGA GGTTTCTGGC CGTCTGGCGA CCCTTCGTAA ACTCAATCCA ACATGCAACT 
    CAGGAATTGT ATCAAAATAT CGCCCATTAC CTTCTTCATG GCAACGTAAA TATACAGAGG 
    GTCACAGCAC TAATACAGCT CGTAATGGGA CAGGACGATT TACTTTTTAG TATGGATGAT 
    GTTCTACAAG AGGTCTTCAG AATACAGCTC TATTTGAATA AGATGCTGCC GCACAACTCT 
    CACAAATGGC AAAAGCCATC CCCCTTTGAC TCCGCAAACT TACTACTTAA CTTCAGAGAC 
    TGGACAACTG ACAATGCTCT CCTCCAAGAG TTGCTACTAT CCTATCCCAC AATTAATAAA 
    AACAAACACA AAAATCACTC CGTCCCTCGT CTAATACAAA TCTGGGTAGA GTCTTATTGG 
    CAAGATAGTG AGACAACATT AAAAGATATC CTCAATTTTT GGTACAGTCA CTTGGCTGAA 
    TATTATGAAT ACCAAGAACT GTTTGCAGAC ATAGTTCAGC TGTTTATAAA CAAAAAAAGA 
    ACGAGGCAAT TGAAGATTCA TTACATTGGT CTAACTGATA AGGAAATCGA AGAAAATAAA 
    CCGCCCCTGG ACTACGAAAA CTTATTTCTC CAATACGAGA TAGACAAAAC GAACGCAAAT 
    GATGAATTGT GCGGTGCAAC TGACCTCAGT GATTTACTTT TCCAATGGAA ACAGGGTGAA 
    CTTCTAGAAG TCGAAGCCTT CGCTCTAAAC GTATCTCCAT GGTCACTTGC AAAGACATTG 
    ACTCTCTTAG AATCTTCTCT TTACTTGGAT ATTGAAACAA TAGAATTCAC AAGACATTTC 
    AAACACAACG ATACAACAAT TGACTCCGTG TTTACGCTTT CCAACCAGTT ATCGTCCTAC 
    GTTCTTGAGA CAACTTTGCA GCAAACGCAC ACCATTTCCT ACTGGTTACA AGTTGCACTG 
    TCTTGTCTAT ACTTACGAAA CTTAAACTCA CTTGCTTCAA TCATTACATC ATTGCAAAAT 
    CATTCAATAG AAAGACTATC TCTCCCGATA GATGTTAAAT CAGACCACCT TTTTCAGCGC 
    CTAAAAGTCG TCGTACATCC AAACAACAAC TACAACGTTT ATAGAAGAAC AATTAAACAT 
    ATTTTCCACA GTCAGCTTCC TTGTGTACCT TTTACATCAC TGCTTATCAG GGACATTACC 
    TTCATAAGAG ACGGAAACGA TACATTCACT AAAGATGGTA ATAACGTGAA TATGCAAAAG 
    TTCAACCAAA TCACAAAGAT AGTCGCTTTT GCGCAATATT TACAACAAAA GCAATATGAA 
    GATATACACT GTTCAAATAC TACTGCAAGA AGCTTATTAG GGGCTATGAT AAAGGTGCAC 
    ACTTTATATA ACGACAACAA AGACAGGGCG TATCAAGTCA GTATAGCTAA GGTTCCAAGG 
    CTTACCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_009967.2
    CDS1..1929
    Translation

    Target ORF information:

    RefSeq Version NM_001178752.1
    Organism Saccharomyces cerevisiae S288C
    Definition Saccharomyces cerevisiae S288C Ras family guanine nucleotide exchange factor BUD5 (BUD5), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001178752.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGAGCCCGA AAAACAAATA TGTATATATC TGTGTAGAAT ATATATATAT ATATTTCGCA 
    AAAATACATA AACAATCAAC CCTCTCCTCA GACACTACTA AGATGTTTGT ACTGATAGAT 
    AATGTCCTCG CCTACCTTCT TGAACAAGAC GACCTATTTG TAACCGCACG CTTCGCTATC 
    CAGGGCCAAA TCGTCTCAAG AAGAGTTAAC AAAATACATA TCTCCAACAT CACAGACGTC 
    CTTCTACAAC AATTCATAAG TCACACACTG CCCTATAATG ACAATATCGT CCCAAAAAAA 
    ATCCTCGACA GCATGAGAAC GGCCGTACGG CAGTTGCTGG AAGCAACTGC CTGTGTCTCT 
    AGAGAATGCC CCCTCGTCAA AAGAAGTCAG GACATAAAAA GAGCAAGAAA ACGTCTACTC 
    AGTGACTGGT ATAGGCTCGG CGCTGATGCA AACATGGATG CCGTATTACT AGTTGTTAAC 
    TCCGCCTGGA GGTTTCTGGC CGTCTGGCGA CCCTTCGTAA ACTCAATCCA ACATGCAACT 
    CAGGAATTGT ATCAAAATAT CGCCCATTAC CTTCTTCATG GCAACGTAAA TATACAGAGG 
    GTCACAGCAC TAATACAGCT CGTAATGGGA CAGGACGATT TACTTTTTAG TATGGATGAT 
    GTTCTACAAG AGGTCTTCAG AATACAGCTC TATTTGAATA AGATGCTGCC GCACAACTCT 
    CACAAATGGC AAAAGCCATC CCCCTTTGAC TCCGCAAACT TACTACTTAA CTTCAGAGAC 
    TGGACAACTG ACAATGCTCT CCTCCAAGAG TTGCTACTAT CCTATCCCAC AATTAATAAA 
    AACAAACACA AAAATCACTC CGTCCCTCGT CTAATACAAA TCTGGGTAGA GTCTTATTGG 
    CAAGATAGTG AGACAACATT AAAAGATATC CTCAATTTTT GGTACAGTCA CTTGGCTGAA 
    TATTATGAAT ACCAAGAACT GTTTGCAGAC ATAGTTCAGC TGTTTATAAA CAAAAAAAGA 
    ACGAGGCAAT TGAAGATTCA TTACATTGGT CTAACTGATA AGGAAATCGA AGAAAATAAA 
    CCGCCCCTGG ACTACGAAAA CTTATTTCTC CAATACGAGA TAGACAAAAC GAACGCAAAT 
    GATGAATTGT GCGGTGCAAC TGACCTCAGT GATTTACTTT TCCAATGGAA ACAGGGTGAA 
    CTTCTAGAAG TCGAAGCCTT CGCTCTAAAC GTATCTCCAT GGTCACTTGC AAAGACATTG 
    ACTCTCTTAG AATCTTCTCT TTACTTGGAT ATTGAAACAA TAGAATTCAC AAGACATTTC 
    AAACACAACG ATACAACAAT TGACTCCGTG TTTACGCTTT CCAACCAGTT ATCGTCCTAC 
    GTTCTTGAGA CAACTTTGCA GCAAACGCAC ACCATTTCCT ACTGGTTACA AGTTGCACTG 
    TCTTGTCTAT ACTTACGAAA CTTAAACTCA CTTGCTTCAA TCATTACATC ATTGCAAAAT 
    CATTCAATAG AAAGACTATC TCTCCCGATA GATGTTAAAT CAGACCACCT TTTTCAGCGC 
    CTAAAAGTCG TCGTACATCC AAACAACAAC TACAACGTTT ATAGAAGAAC AATTAAACAT 
    ATTTTCCACA GTCAGCTTCC TTGTGTACCT TTTACATCAC TGCTTATCAG GGACATTACC 
    TTCATAAGAG ACGGAAACGA TACATTCACT AAAGATGGTA ATAACGTGAA TATGCAAAAG 
    TTCAACCAAA TCACAAAGAT AGTCGCTTTT GCGCAATATT TACAACAAAA GCAATATGAA 
    GATATACACT GTTCAAATAC TACTGCAAGA AGCTTATTAG GGGCTATGAT AAAGGTGCAC 
    ACTTTATATA ACGACAACAA AGACAGGGCG TATCAAGTCA GTATAGCTAA GGTTCCAAGG 
    CTTACCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    Life with 6000 genes.
    Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
    Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG


    The complete DNA sequence of yeast chromosome III.
    Nature357(6373)38-46(1992 May)
    Oliver SG,van der Aart QJ,Agostoni-Carbone ML,Aigle M,Alberghina L,Alexandraki D,Antoine G,Anwar R,Ballesta JP,Benit P


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