BUD3 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	BUD3
Entrez Gene ID	850345
Full Name	Bud3p
Synonyms	YCL012W
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001178662.1	NP_009914.2	Bud3p

Pathways from BioSystems

Homologies

Eremothecium gossypii	AGOS_AAL016C	NP_982526.1
Saccharomyces cerevisiae (baker's yeast)	BUD3	NP_009914.2
Kluyveromyces lactis	KLLA0D04994g	XP_453282.1

Gene Ontology
Bibliography

Related articles in PubMed

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

The complete DNA sequence of yeast chromosome III.
Oliver SG, van der Aart QJ, Agostoni-Carbone ML, Aigle M, Alberghina L, Alexandraki D, Antoine G, Anwar R, Ballesta JP, Benit P
Nature357(6373)38-46(1992 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Cell polarization in Saccharomyces cerevisiae, to a proper growth site, requires activation of Cdc42 by Bud3.
Title: Bud3 activates Cdc42 to establish a proper growth site in budding yeast.

The following BUD3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the BUD3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi00068	NM_001178662.1 Latest version!	Saccharomyces cerevisiae S288C Bud3p (BUD3), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

BUD3 ( NM_001178662.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_009914.2 Saccharomyces cerevisiae S288C Bud3p (BUD3), partial mRNA.

Price & Availability↑

CloneID	OSi00068
Clone ID Related Accession (Same CDS sequence)	NM_001178662.1
Accession Version	NM_001178662.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4911bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	Bud3p
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001135). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGGAGAAAG ACCTGTCGTC TCTTTACTCT GAAAAGAAAG ACAAAGAGAA CGATGAAACC 
TTATTTAACA TCAAACTATC CAAATCTGTT GTCGAGACCA CACCGCTAAA TGGTCATTCA 
TTGTTTGATG ATGATAAATC ACTTTCAGAC TGGACGGATA ATGTGTTCAC TCAATCAGTA 
TTCTATCACG GGTCAGATGA CTTGATATGG GGGAAGTTCT TTGTCTGCGT GTACAAGTCC 
CCCAACAGCA ATAAGTTGAA CGCTATAATA TTCGACAAAT TAGGAACATC ATGCTTCGAA 
TCCGTCGATA TATCTTCCAA CTCGCAATAC TATCCGGCCA TTGAGAATTT GAGTCCAAGT 
GATCAGGAAA GCAATGTTAA GAAATGCATT GCTGTCATTC TGTTACAGCG CTATCCATTA 
CTTTCACCAT CAGACTTATC ACAAATATTG TCCAATAAAT CGGAAAATTG CGACTATGAC 
CCCCCTTATG CTGGAGATTT GGCTAGTAGT TGCCAGTTGA TAACAGCAGT TCCTCCAGAA 
GATCTGGGGA AGCGCTTCTT TACATCAGGA CTTCTGCAAA ATAGATTTGT CAGCTCTACC 
CTGTTAGATG TTATTTATGA AAACAATGAA TCCACCATCG AACTAAATAA TAGGTTGGTA 
TTCCATCTGG GTGAACAACT TGAACAACTT TTTAACCCAG TCACAGAATA CTCACCGGAA 
CAGACAGAAT ATGGTTATAA GGCGCCAGAG GACGAATTAC CCACAGAATC GGATGATGAT 
CTTGTCAAGG CCATTTGCAA CGAGTTATTA CAACTACAAA CAAATTTTAC TTTCAATTTG 
GTAGAATTTT TGCAAAAATT CCTGATCGCC TTGAGAGTCA GAGTACTCAA TGAAGAAATT 
AATGGGTTAT CCACAACCAA ATTAAATCGA CTCTTCCCAC CTACAATAGA TGAAGTCACA 
AGAATCAATT GTATTTTTCT AGACTCGCTA AAGACAGCAA TCCCTTACGG TTCCCTCGAA 
GTACTGAAGG CATGCAGCAT TACTATTCCT TATTTCTACA AAGCATATAC AAGACACGAG 
GCGGCCACAA AGAACTTCAG CAAAGATATT AAATTGTTTA TTAGGCATTT CAGCAATGTA 
ATTCCAGAAA GAGAGGTCTA CACGGAAATG AAAATCGAGA GTATAATTAA GGGACCTCAG 
GAAAAACTAC TGAAGCTAAA GTTAATTATA GAGAGATTGT GGAAGTCGAA AAAATGGAGA 
CCGAAAAATC AAGAAATGGC AAAAAAATGC TACAACAATA TCATTGATGT CATTGATTCG 
TTTGGAAAAT TAGATTCCCC ACTTCATTCT TATAGTACCA GAGTATTTAC TCCATCGGGA 
AAAATCCTTA CAGAATTAGC CAAATGCTGG CCCGTAGAAC TGCAATACAA ATGGCTGAAG 
AGAAGGGTAG TCGGTGTGTA TGATGTAGTG GATTTGAATG ATGAAAATAA GAGAAATTTA 
TTAGTCATAT TCAGTGATTA TGTGGTTTTC ATCAATATAC TGGAGGCAGA AAGTTACTAC 
ACTTCAGATG GATCAAACAG GCCCTTAATC TCAGATATTT TAATGAACTC ATTGATCAAC 
GAAGTTCCGT TGCCCTCCAA GATCCCTAAG TTGAAAGTGG AGCGTCATTG CTATATAGAT 
GAGGTTCTAG TTTCTATATT AGACAAAAGC ACTCTACGTT TTGATCGATT GAAGGGAAAA 
GATTCTTTCT CAATGGTATG TAAATTATCC TCTGCATTTA TCTCTTCTTC GTCAGTTGCT 
GACTTGATTA CGAAGGCTAG AATTTTGGAA AAAGACACTG CATTTCATTT ATTTAAAGCT 
AGTAGAAGCC ATTTTACATT ATATTCTACT GCTCACGAGC TTTGCGCTTA TGATTCCGAA 
AAAATAAAAT CAAAATTTGC CTTATTCCTG AACATACCAC CATCCAAGGA GATATTGGAG 
GTCAACAACC TTCATTTGGC TTTTTTTGCA AGATTTTGCA GTAACGATGG TAGAGATAAC 
ATCGTAATCT TAGACGTCTT AACCAAACAT GACGATAAAC ATATAGAAGT TACATCCGAT 
AACATTGTTT TCACCATAAT TAATCAATTG GCCATTGAAA TACCGATATG CTTTTCTTCC 
TTAAACTCAT CGATGGCCAA AGATTTACTC TGTGTAAATG AGAATTTGAT AAAAAACTTA 
GAACATCAAT TGGAAGAGGT CAAGCACCCT TCAACAGACG AACATAGGGC TGTTAATAGC 
AAACTTTCCG GTGCATCCGA TTTCGATGCT ACTCACGAGA AGAAAAGATC ATACGGTACC 
ATAACAACAT TTAGAAGCTA TACAAGCGAC TTGAAGGACA GTCCATCAGG CGATAATAGT 
AATGTCACCA AGGAAACTAA GGAAATTTTA CCAGTGAAAC CTACGAAAAA GTCTTCAAAA 
AAACCAAGAG AAATTCAAAA GAAGACCAAG ACAAACGCCT CTAAAGCAGA GCACATAGAA 
AAGAAGAAGC CTAACAAAGG CAAAGGGTTT TTTGGCGTGT TAAAAAATGT TTTTGGAAGT 
AAAAGCAAGA GCAAGCCTTC ACCAGTTCAA AGAGTGCCTA AAAAAATATC GCAGAGGCAT 
CCTAAGTCTC CAGTGAAGAA GCCAATGACC TCAGAAAAGA AATCCTCCCC TAAAAGGGCA 
GTCGTTTCAT CTCCCAAAAT TAAAAAGAAA AGTACTTCTT TTTCCACAAA AGAATCACAA 
ACTGCTAAAT CTTCTCTTCG AGCAGTTGAA TTCAAATCTG ATGACTTGAT CGGAAAACCA 
CCTGATGTTG GAAATGGCGC ACATCCTCAA GAAAATACCA GAATATCTTC AGTAGTAAGG 
GATACAAAAT ATGTCTCCTA CAATCCCTCT CAGCCTGTGA CAGAAAATAC CAGTAACGAA 
AAAAATGTCG AACCAAAAGC GGATCAATCC ACAAAGCAGG ATAACATTTC CAATTTTGCA 
GATGTAGAGG TATCTGCGTC TTCTTATCCT GAAAAACTTG ATGCAGAAAC AGATGATCAA 
ATAATTGGGA AGGCGACGAA TTCGTCATCA GTTCATGGAA ATAAAGAGCT GCCAGACCTT 
GCTGAGGTGA CTACAGCAAA TAGGGTTTCT ACAACATCGG CTGGGGACCA ACGTATTGAT 
ACCCAAAGCG AATTTTTACG TGCAGCTGAT GTTGAAAACT TAAGTGATGA CGATGAACAC 
AGACAGAATG AAAGTAGAGT TTTTAACGAT GACCTCTTTG GTGATTTTAT TCCTAAGCAT 
TACCGTAATA AACAGGAGAA CATTAACAGC TCGAGTAATT TGTTTCCAGA GGGAAAGGTG 
CCCCAAGAAA AGGGCGTATC AAATGAAAAC ACTAACATAT CTCTCAAAAC TAATGAAGAT 
GCATCTACAT TGACGCAGAA ACTCTCTCCA CAAGCGAGTA AAGTGCTGAC AGAAAATTCT 
AATGAATTAA AAGATACCAA CAATGAAGGG AAGGACGCAA AGGACATAAA ATTAGGAGAT 
GATTACAGTG ATAAAGAAAC AGCGAAAGAA ATAACTAAAC CAAAAAATTT TGTTGAAGGA 
ATAACTGAAC GGAAAGAAAT ATTCCCCACT ATTCCTAGGT TAGCGCCGCC AGCTTCAAAA 
ATTAACTTTC AAAGGTCACC ATCCTATATT GAGCTCTTTC AAGGAATGAG GGTGGTTTTA 
GATAAGCATG ATGCCCATTA TAACTGGAAA CGCTTGGCTA GTCAAGTCTC CTTAAGTGAG 
GGACTAAAAG TCAATACTGA GGAAGATGCG GCAATTATAA ATAAAAGTCA GGATGATGCC 
AAGGCGGAAA GAATGACTCA AATTTCTGAA GTGATTGAGT ATGAAATGCA GCAACCTATC 
CCAACTTATT TGCCTAAGGC GCATCTAGAT GACTCGGGTA TTGAAAAAAG TGATGACAAA 
TTCTTCGAAA TTGAAGAAGA ACTTAAGGAA GAATTGAAGG GCAGCAAAAC CGGTAATGAA 
GATGTCGGTA ATAATAATCC ATCCAATTCT ATTCCAAAAA TCGAGAAGCC CCCAGCATTC 
AAAGTTATTA GAACATCGCC TGTGAGAATT ATCGGAAGGA CTTTTGAAGA CACTAGAAAA 
TATGAAAATG GCTCTCCATC TGATATTTCG TTCACTTATG ATACTCACAA CAATGATGAA 
CCTGACAAAA GGCTGATGGA ATTAAAATTT CCATCCCAAG ATGAAATTCC GGATGACAGA 
TTCTATACTC CAGCAGAGGA ACCCACTGCT GAATTTCCGG TGGAAGAACT TCCAAATACT 
CCGCGAAGTA TTAACGTTAC AACTTCAAAT AACAAGAGCA CAGACGATAA GTTGAGTAGC 
GGTAATATTG ATCAAAAACC TACCGAACTG TTAGATGATT TAGAATTCAG TTCATTTAAT 
ATAGCATTTG GAAATACCTC CATGAGTACT GACAATATGA AAATATCATC CGACTTAAGT 
TCGAATAAAA CCGTGTTAGG AAATGCTCAG AAAGTTCAAG AGTCTCCTAG TGGACCATTA 
ATCTATGTTT TGCCTCAGAG TAGCACAAAG CATGAGAAAG AGGGGTTCCT TCGAAAGAAA 
CAAAAAGACG AGCCCATTTG GGTTTCCCCT AGCAAAATTG ACTTTGCTGA TCTAAGTAGG 
AGAACTAAAG CATTGACGCC AGAGCGTAAT ACTGTTCCTT TGAAAAACAA CGACAGTAGA 
AAATACAAAT ATACTGGAGA GGGATCTATC GGTAATATGA CAAATATGCT GTTAACTAAA 
GATGCTTCGT ACGCGTACTT AAAAGATTTT GTTGCGTTGA GTGACGATGA AGATGAAGAT 
GGGAAACAGA ACTGCGCTGT TGGTGGCCCA GAGAAACTGA AATTTTATTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_009914.2
CDS	1..4911
Translation	MEKDLSSLYSEKKDKENDETLFNIKLSKSVVETTPLNGHSLFDDDKSLSDWTDNVFTQSVFYHGSDDLIWGKFFVCVYKSPNSNKLNAIIFDKLGTSCFESVDISSNSQYYPAIENLSPSDQESNVKKCIAVILLQRYPLLSPSDLSQILSNKSENCDYDPPYAGDLASSCQLITAVPPEDLGKRFFTSGLLQNRFVSSTLLDVIYENNESTIELNNRLVFHLGEQLEQLFNPVTEYSPEQTEYGYKAPEDELPTESDDDLVKAICNELLQLQTNFTFNLVEFLQKFLIALRVRVLNEEINGLSTTKLNRLFPPTIDEVTRINCIFLDSLKTAIPYGSLEVLKACSITIPYFYKAYTRHEAATKNFSKDIKLFIRHFSNVIPEREVYTEMKIESIIKGPQEKLLKLKLIIERLWKSKKWRPKNQEMAKKCYNNIIDVIDSFGKLDSPLHSYSTRVFTPSGKILTELAKCWPVELQYKWLKRRVVGVYDVVDLNDENKRNLLVIFSDYVVFINILEAESYYTSDGSNRPLISDILMNSLINEVPLPSKIPKLKVERHCYIDEVLVSILDKSTLRFDRLKGKDSFSMVCKLSSAFISSSSVADLITKARILEKDTAFHLFKASRSHFTLYSTAHELCAYDSEKIKSKFALFLNIPPSKEILEVNNLHLAFFARFCSNDGRDNIVILDVLTKHDDKHIEVTSDNIVFTIINQLAIEIPICFSSLNSSMAKDLLCVNENLIKNLEHQLEEVKHPSTDEHRAVNSKLSGASDFDATHEKKRSYGTITTFRSYTSDLKDSPSGDNSNVTKETKEILPVKPTKKSSKKPREIQKKTKTNASKAEHIEKKKPNKGKGFFGVLKNVFGSKSKSKPSPVQRVPKKISQRHPKSPVKKPMTSEKKSSPKRAVVSSPKIKKKSTSFSTKESQTAKSSLRAVEFKSDDLIGKPPDVGNGAHPQENTRISSVVRDTKYVSYNPSQPVTENTSNEKNVEPKADQSTKQDNISNFADVEVSASSYPEKLDAETDDQIIGKATNSSSVHGNKELPDLAEVTTANRVSTTSAGDQRIDTQSEFLRAADVENLSDDDEHRQNESRVFNDDLFGDFIPKHYRNKQENINSSSNLFPEGKVPQEKGVSNENTNISLKTNEDASTLTQKLSPQASKVLTENSNELKDTNNEGKDAKDIKLGDDYSDKETAKEITKPKNFVEGITERKEIFPTIPRLAPPASKINFQRSPSYIELFQGMRVVLDKHDAHYNWKRLASQVSLSEGLKVNTEEDAAIINKSQDDAKAERMTQISEVIEYEMQQPIPTYLPKAHLDDSGIEKSDDKFFEIEEELKEELKGSKTGNEDVGNNNPSNSIPKIEKPPAFKVIRTSPVRIIGRTFEDTRKYENGSPSDISFTYDTHNNDEPDKRLMELKFPSQDEIPDDRFYTPAEEPTAEFPVEELPNTPRSINVTTSNNKSTDDKLSSGNIDQKPTELLDDLEFSSFNIAFGNTSMSTDNMKISSDLSSNKTVLGNAQKVQESPSGPLIYVLPQSSTKHEKEGFLRKKQKDEPIWVSPSKIDFADLSRRTKALTPERNTVPLKNNDSRKYKYTGEGSIGNMTNMLLTKDASYAYLKDFVALSDDEDEDGKQNCAVGGPEKLKFY

Target ORF information:

RefSeq Version	NM_001178662.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C Bud3p (BUD3), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001178662.1

ORF Insert Sequence:

ATGGAGAAAG ACCTGTCGTC TCTTTACTCT GAAAAGAAAG ACAAAGAGAA CGATGAAACC 
TTATTTAACA TCAAACTATC CAAATCTGTT GTCGAGACCA CACCGCTAAA TGGTCATTCA 
TTGTTTGATG ATGATAAATC ACTTTCAGAC TGGACGGATA ATGTGTTCAC TCAATCAGTA 
TTCTATCACG GGTCAGATGA CTTGATATGG GGGAAGTTCT TTGTCTGCGT GTACAAGTCC 
CCCAACAGCA ATAAGTTGAA CGCTATAATA TTCGACAAAT TAGGAACATC ATGCTTCGAA 
TCCGTCGATA TATCTTCCAA CTCGCAATAC TATCCGGCCA TTGAGAATTT GAGTCCAAGT 
GATCAGGAAA GCAATGTTAA GAAATGCATT GCTGTCATTC TGTTACAGCG CTATCCATTA 
CTTTCACCAT CAGACTTATC ACAAATATTG TCCAATAAAT CGGAAAATTG CGACTATGAC 
CCCCCTTATG CTGGAGATTT GGCTAGTAGT TGCCAGTTGA TAACAGCAGT TCCTCCAGAA 
GATCTGGGGA AGCGCTTCTT TACATCAGGA CTTCTGCAAA ATAGATTTGT CAGCTCTACC 
CTGTTAGATG TTATTTATGA AAACAATGAA TCCACCATCG AACTAAATAA TAGGTTGGTA 
TTCCATCTGG GTGAACAACT TGAACAACTT TTTAACCCAG TCACAGAATA CTCACCGGAA 
CAGACAGAAT ATGGTTATAA GGCGCCAGAG GACGAATTAC CCACAGAATC GGATGATGAT 
CTTGTCAAGG CCATTTGCAA CGAGTTATTA CAACTACAAA CAAATTTTAC TTTCAATTTG 
GTAGAATTTT TGCAAAAATT CCTGATCGCC TTGAGAGTCA GAGTACTCAA TGAAGAAATT 
AATGGGTTAT CCACAACCAA ATTAAATCGA CTCTTCCCAC CTACAATAGA TGAAGTCACA 
AGAATCAATT GTATTTTTCT AGACTCGCTA AAGACAGCAA TCCCTTACGG TTCCCTCGAA 
GTACTGAAGG CATGCAGCAT TACTATTCCT TATTTCTACA AAGCATATAC AAGACACGAG 
GCGGCCACAA AGAACTTCAG CAAAGATATT AAATTGTTTA TTAGGCATTT CAGCAATGTA 
ATTCCAGAAA GAGAGGTCTA CACGGAAATG AAAATCGAGA GTATAATTAA GGGACCTCAG 
GAAAAACTAC TGAAGCTAAA GTTAATTATA GAGAGATTGT GGAAGTCGAA AAAATGGAGA 
CCGAAAAATC AAGAAATGGC AAAAAAATGC TACAACAATA TCATTGATGT CATTGATTCG 
TTTGGAAAAT TAGATTCCCC ACTTCATTCT TATAGTACCA GAGTATTTAC TCCATCGGGA 
AAAATCCTTA CAGAATTAGC CAAATGCTGG CCCGTAGAAC TGCAATACAA ATGGCTGAAG 
AGAAGGGTAG TCGGTGTGTA TGATGTAGTG GATTTGAATG ATGAAAATAA GAGAAATTTA 
TTAGTCATAT TCAGTGATTA TGTGGTTTTC ATCAATATAC TGGAGGCAGA AAGTTACTAC 
ACTTCAGATG GATCAAACAG GCCCTTAATC TCAGATATTT TAATGAACTC ATTGATCAAC 
GAAGTTCCGT TGCCCTCCAA GATCCCTAAG TTGAAAGTGG AGCGTCATTG CTATATAGAT 
GAGGTTCTAG TTTCTATATT AGACAAAAGC ACTCTACGTT TTGATCGATT GAAGGGAAAA 
GATTCTTTCT CAATGGTATG TAAATTATCC TCTGCATTTA TCTCTTCTTC GTCAGTTGCT 
GACTTGATTA CGAAGGCTAG AATTTTGGAA AAAGACACTG CATTTCATTT ATTTAAAGCT 
AGTAGAAGCC ATTTTACATT ATATTCTACT GCTCACGAGC TTTGCGCTTA TGATTCCGAA 
AAAATAAAAT CAAAATTTGC CTTATTCCTG AACATACCAC CATCCAAGGA GATATTGGAG 
GTCAACAACC TTCATTTGGC TTTTTTTGCA AGATTTTGCA GTAACGATGG TAGAGATAAC 
ATCGTAATCT TAGACGTCTT AACCAAACAT GACGATAAAC ATATAGAAGT TACATCCGAT 
AACATTGTTT TCACCATAAT TAATCAATTG GCCATTGAAA TACCGATATG CTTTTCTTCC 
TTAAACTCAT CGATGGCCAA AGATTTACTC TGTGTAAATG AGAATTTGAT AAAAAACTTA 
GAACATCAAT TGGAAGAGGT CAAGCACCCT TCAACAGACG AACATAGGGC TGTTAATAGC 
AAACTTTCCG GTGCATCCGA TTTCGATGCT ACTCACGAGA AGAAAAGATC ATACGGTACC 
ATAACAACAT TTAGAAGCTA TACAAGCGAC TTGAAGGACA GTCCATCAGG CGATAATAGT 
AATGTCACCA AGGAAACTAA GGAAATTTTA CCAGTGAAAC CTACGAAAAA GTCTTCAAAA 
AAACCAAGAG AAATTCAAAA GAAGACCAAG ACAAACGCCT CTAAAGCAGA GCACATAGAA 
AAGAAGAAGC CTAACAAAGG CAAAGGGTTT TTTGGCGTGT TAAAAAATGT TTTTGGAAGT 
AAAAGCAAGA GCAAGCCTTC ACCAGTTCAA AGAGTGCCTA AAAAAATATC GCAGAGGCAT 
CCTAAGTCTC CAGTGAAGAA GCCAATGACC TCAGAAAAGA AATCCTCCCC TAAAAGGGCA 
GTCGTTTCAT CTCCCAAAAT TAAAAAGAAA AGTACTTCTT TTTCCACAAA AGAATCACAA 
ACTGCTAAAT CTTCTCTTCG AGCAGTTGAA TTCAAATCTG ATGACTTGAT CGGAAAACCA 
CCTGATGTTG GAAATGGCGC ACATCCTCAA GAAAATACCA GAATATCTTC AGTAGTAAGG 
GATACAAAAT ATGTCTCCTA CAATCCCTCT CAGCCTGTGA CAGAAAATAC CAGTAACGAA 
AAAAATGTCG AACCAAAAGC GGATCAATCC ACAAAGCAGG ATAACATTTC CAATTTTGCA 
GATGTAGAGG TATCTGCGTC TTCTTATCCT GAAAAACTTG ATGCAGAAAC AGATGATCAA 
ATAATTGGGA AGGCGACGAA TTCGTCATCA GTTCATGGAA ATAAAGAGCT GCCAGACCTT 
GCTGAGGTGA CTACAGCAAA TAGGGTTTCT ACAACATCGG CTGGGGACCA ACGTATTGAT 
ACCCAAAGCG AATTTTTACG TGCAGCTGAT GTTGAAAACT TAAGTGATGA CGATGAACAC 
AGACAGAATG AAAGTAGAGT TTTTAACGAT GACCTCTTTG GTGATTTTAT TCCTAAGCAT 
TACCGTAATA AACAGGAGAA CATTAACAGC TCGAGTAATT TGTTTCCAGA GGGAAAGGTG 
CCCCAAGAAA AGGGCGTATC AAATGAAAAC ACTAACATAT CTCTCAAAAC TAATGAAGAT 
GCATCTACAT TGACGCAGAA ACTCTCTCCA CAAGCGAGTA AAGTGCTGAC AGAAAATTCT 
AATGAATTAA AAGATACCAA CAATGAAGGG AAGGACGCAA AGGACATAAA ATTAGGAGAT 
GATTACAGTG ATAAAGAAAC AGCGAAAGAA ATAACTAAAC CAAAAAATTT TGTTGAAGGA 
ATAACTGAAC GGAAAGAAAT ATTCCCCACT ATTCCTAGGT TAGCGCCGCC AGCTTCAAAA 
ATTAACTTTC AAAGGTCACC ATCCTATATT GAGCTCTTTC AAGGAATGAG GGTGGTTTTA 
GATAAGCATG ATGCCCATTA TAACTGGAAA CGCTTGGCTA GTCAAGTCTC CTTAAGTGAG 
GGACTAAAAG TCAATACTGA GGAAGATGCG GCAATTATAA ATAAAAGTCA GGATGATGCC 
AAGGCGGAAA GAATGACTCA AATTTCTGAA GTGATTGAGT ATGAAATGCA GCAACCTATC 
CCAACTTATT TGCCTAAGGC GCATCTAGAT GACTCGGGTA TTGAAAAAAG TGATGACAAA 
TTCTTCGAAA TTGAAGAAGA ACTTAAGGAA GAATTGAAGG GCAGCAAAAC CGGTAATGAA 
GATGTCGGTA ATAATAATCC ATCCAATTCT ATTCCAAAAA TCGAGAAGCC CCCAGCATTC 
AAAGTTATTA GAACATCGCC TGTGAGAATT ATCGGAAGGA CTTTTGAAGA CACTAGAAAA 
TATGAAAATG GCTCTCCATC TGATATTTCG TTCACTTATG ATACTCACAA CAATGATGAA 
CCTGACAAAA GGCTGATGGA ATTAAAATTT CCATCCCAAG ATGAAATTCC GGATGACAGA 
TTCTATACTC CAGCAGAGGA ACCCACTGCT GAATTTCCGG TGGAAGAACT TCCAAATACT 
CCGCGAAGTA TTAACGTTAC AACTTCAAAT AACAAGAGCA CAGACGATAA GTTGAGTAGC 
GGTAATATTG ATCAAAAACC TACCGAACTG TTAGATGATT TAGAATTCAG TTCATTTAAT 
ATAGCATTTG GAAATACCTC CATGAGTACT GACAATATGA AAATATCATC CGACTTAAGT 
TCGAATAAAA CCGTGTTAGG AAATGCTCAG AAAGTTCAAG AGTCTCCTAG TGGACCATTA 
ATCTATGTTT TGCCTCAGAG TAGCACAAAG CATGAGAAAG AGGGGTTCCT TCGAAAGAAA 
CAAAAAGACG AGCCCATTTG GGTTTCCCCT AGCAAAATTG ACTTTGCTGA TCTAAGTAGG 
AGAACTAAAG CATTGACGCC AGAGCGTAAT ACTGTTCCTT TGAAAAACAA CGACAGTAGA 
AAATACAAAT ATACTGGAGA GGGATCTATC GGTAATATGA CAAATATGCT GTTAACTAAA 
GATGCTTCGT ACGCGTACTT AAAAGATTTT GTTGCGTTGA GTGACGATGA AGATGAAGAT 
GGGAAACAGA ACTGCGCTGT TGGTGGCCCA GAGAAACTGA AATTTTATTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

The complete DNA sequence of yeast chromosome III.
Nature357(6373)38-46(1992 May)
Oliver SG,van der Aart QJ,Agostoni-Carbone ML,Aigle M,Alberghina L,Alexandraki D,Antoine G,Anwar R,Ballesta JP,Benit P

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BUD3 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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