Acsl5 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Acsl5
Entrez Gene ID	94340
Full Name	acyl-CoA synthetase long-chain family member 5
Synonyms	Acs5,Facl5
General protein information	Preferred Names acyl-CoA synthetase long-chain family member 5 Names long-chain-fatty-acid--CoA ligase 5 LACS 5 fatty acid Coenzyme A ligase, long chain 5 long-chain acyl-CoA synthetase 5 long-chain fatty acid coenzyme A ligase 5
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 1 Map Location: 1q55
Summary	catalyzes the ligation of a fatty acid to produce acyl Co-A; has preferential activity for C16-C18 unsaturated fatty acids [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_053607.1	NP_446059.1	long-chain-fatty-acid--CoA ligase 5
XM_017589808.1	XP_017445297.1	long-chain-fatty-acid--CoA ligase 5 isoform X1

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	CG3961	NP_730369.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011603	XP_320900.3
Arabidopsis thaliana (thale cress)	LACS6	NP_566265.1
Oryza sativa (rice)	Os11g0147000	NP_001065739.1
Oryza sativa (rice)	Os12g0143900	NP_001066139.1
Canis lupus familiaris (dog)	ACSL5	XP_005637786.1
Mus musculus (house mouse)	Acsl5	NP_082252.1
Homo sapiens (human)	ACSL5	NP_057318.2
Bos taurus (cattle)	ACSL5	NP_001069118.1
Danio rerio (zebrafish)	acsl5	NP_001004599.1
Caenorhabditis elegans (roundworm)	acs-13	NP_490744.1
Pan troglodytes (chimpanzee)	ACSL5	XP_001146649.1
Rattus norvegicus (Norway rat)	Acsl5	NP_446059.1
Gallus gallus (chicken)	ACSL5	NP_001026408.1
Xenopus tropicalis (tropical clawed frog)	acsl5	NP_001011069.1

Gene Ontology
Bibliography

Related articles in PubMed

Long-chain acyl-CoA synthetase isoforms differ in preferences for eicosanoid species and long-chain fatty acids.
Klett EL, Chen S, Yechoor A, Lih FB, Coleman RA
Journal of lipid research58(5)884-894(2017 May)

Identification of acyl-CoA synthetases involved in the mammalian sphingosine 1-phosphate metabolic pathway.
Ohkuni A, Ohno Y, Kihara A
Biochemical and biophysical research communications442(3-4)195-201(2013 Dec)

The Sj?gren-Larsson syndrome gene encodes a hexadecenal dehydrogenase of the sphingosine 1-phosphate degradation pathway.
Nakahara K, Ohkuni A, Kitamura T, Abe K, Naganuma T, Ohno Y, Zoeller RA, Kihara A
Molecular cell46(4)461-71(2012 May)

Human intestinal acyl-CoA synthetase 5 is sensitive to the inhibitor triacsin C.
Kaemmerer E, Peuscher A, Reinartz A, Liedtke C, Weiskirchen R, Kopitz J, Gassler N
World journal of gastroenterology17(44)4883-9(2011 Nov)

Hepatic long-chain acyl-CoA synthetase 5 mediates fatty acid channeling between anabolic and catabolic pathways.
Bu SY, Mashek DG
Journal of lipid research51(11)3270-80(2010 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The data strongly indicate that human but not rat acyl-CoA synthetase 5 is sensitive to triacsin C and does not compensate for other triacsin C-sensitive ACSL isoforms.
Title: Human intestinal acyl-CoA synthetase 5 is sensitive to the inhibitor triacsin C.

ACSL5 activates and channels fatty acids toward anabolic pathways
Title: Hepatic long-chain acyl-CoA synthetase 5 mediates fatty acid channeling between anabolic and catabolic pathways.

uptake of fatty acids into cells is regulated by metabolism and overexpressed ACSL5 partitions exogenously derived fatty acids toward TAG synthesis and storage
Title: Rat long chain acyl-CoA synthetase 5 increases fatty acid uptake and partitioning to cellular triacylglycerol in McArdle-RH7777 cells.

The following Acsl5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Acsl5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa10061	NM_053607.1 Latest version!	Rattus norvegicus acyl-CoA synthetase long-chain family member 5 (Acsl5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
ORa56884	XM_017589808.1 Latest version!	Rattus norvegicus acyl-CoA synthetase long-chain family member 5 (Acsl5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Acsl5 ( NM_053607.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_446059.1 Rattus norvegicus acyl-CoA synthetase long-chain family member 5 (Acsl5), mRNA.

Price & Availability↑

CloneID	ORa10061
Clone ID Related Accession (Same CDS sequence)	NM_053607.1
Accession Version	NM_053607.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2052bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Rattus norvegicus(Norway rat)
Product	long-chain-fatty-acid--CoA ligase 5
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AB012933.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB012933.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2689598, SAMEA2689599 [ECO:0000350] ##Evidence-Data-END##

ATGCTTTTTA TTTTTAACTT CTTGTTTTCC CCACTTCCGA CCCCGGCCCT GATCTGCCTC 
CTGACATTTG GAACGGCTAT CTTCCTGTGG CTGATCAACA GACCTCAGCC AGTCTTACCT 
CTCATTGACT TGGACAACCA GTCTGTAGGG ATTGAGGGAG GAGCACGGAG AGGCGCTTTC 
CAGAAGAACA ATGACCTAAT CCTTTATTAC TTCTCAGACG CCAAGACGTT GTATGAAGTT 
TTCCAAAGAG GACTTGCTGT GTCTGACAAT GGACCTTGCT TGGGATACAG AAAGCCGAAC 
CAGCCCTACA AGTGGATATC CTACAAGCAG GTGTCTGATC GAGCAGAGTA CCTGGGCTCC 
TGTCTTTTGC ATAAAGGATA CAAGCCATCG CAGGACCAAT TTATTGGCAT CTTTGCTCAA 
AATAGGCCAG AGTGGGTCAT CTCCGAGTTA GCCTGTTACA CATATTCCAT GGTAGCTGTC 
CCCCTGTATG ACACACTGGG AGCAGAAGCC ATCATCTATG TCATCAACAG AGCTGATATC 
TCCGTGGTGA TCTGTGATAC ACCCCAAAAA GCAACAATGC TGATAGAAAA TGTGGAAAAG 
GACCTTACCC CAGGCCTGAA GACAGTCATC CTCATGGACC CTTTCGATGA TGACCTGATG 
AAACGAGGAG AGAAGTGTGG CATTGAGATG TTGTCTCTGC ATGATGCTGA GAATCTAGGC 
AAAGAGAACT TCAAAAAACC AATGCCTCCG AACCCAGAGG ATCTAAGTGT TATCTGTTTC 
ACCAGTGGAA CTACAGGTGA CCCCAAAGGA GCTATGCTGA CCCATCAAAA TATCGTTTCA 
AACATGGCTG CTTTCCTCAA ATTTCTGGAG CCTATCTTCC AGCCCACCCC CGAGGATGTG 
ACCATATCCT ACCTTCCCTT GGCCCATATG TTTGAGAGGC TTGTCCAGGG TGTCATATTT 
TCCTGTGGAG GCAAAATTGG GTTCTTCCAA GGAGATATCC GGTTGCTACC TGATGACATG 
AAGGCTTTGA AACCCACAGT GTTTCCCACA GTGCCTCGAC TCCTTAACAG GGTCTATGAT 
AAGGTACAAA ATGAAGCCAA GACACCTTTG AAAAAGTTCT TATTGAACTT GGCTATCATC 
AGCAAATTCA ACGAAGTGAG AAATGGCATC ATTCGGCGGA ACAGTTTGTG GGACAAGCTC 
GTGTTTTCAA AGATCCAAAG CAGCCTGGGT GGGAAGGTTC GTCTCATGAT CACTGGAGCC 
GCCCCTATCT CCACTCCAGT CTTGACGTTC TTCAGGGCTG CAATGGGATG CTGGGTGTTT 
GAAGCTTACG GCCAAACAGA ATGCACAGCT GGGTGTTCCA TTACATCACC TGGGGACTGG 
ACAGCAGGTC ATGTTGGGAC TCCGGTGTCC TGCAATTTTG TCAAGCTGGA AGATGTGGCT 
GACATGAACT ACTTTTCAGT AAACAACGAA GGCGAGATCT GCATCAAAGG CAACAATGTG 
TTCAAAGGCT ACCTAAAGGA CCCAGAGAAG ACACAGGAAG TACTGGACAA GGATGGCTGG 
CTTCACACTG GGGACATCGG TCGCTGGCTT CCAAATGGAA CTCTGAAAAT CATTGACCGG 
AAGAAGAATA TTTTCAAATT GGCACAAGGA GAATACATCG CTCCAGAGAA GATTGAAAAT 
GTTTATTCCA GGAGCAGACC AATATTGCAA GTTTTTGTCC ATGGGGAGAG CTTACGGTCA 
TTCCTGATAG GAGTGGTGGT TCCTGACCCA GAGTCACTTC CCTCATTTGC AGCCAAGATC 
GGGGTAAAGG GATCCTTTGA AGAACTATGC CAAAACCAGT GTGTAAAGAA AGCCATTTTA 
GAAGATTTGC AGAAAGTTGG GAAAGAAGGT GGCCTTAAAT CCTTTGAGCA GGTCAAGAGC 
ATCTTTGTGC ATCCGGAGCC GTTCTCAATT GAAAACGGGC TTCTGACACC GACACTGAAA 
GCCAAACGAG TGGAGCTTGC CAAGTTCTTC CAGACACAAA TCAAGAGCCT CTATGAGAGC 
ATCGAAGAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_446059.1
CDS	121..2172
Translation	MLFIFNFLFSPLPTPALICLLTFGTAIFLWLINRPQPVLPLIDLDNQSVGIEGGARRGAFQKNNDLILYYFSDAKTLYEVFQRGLAVSDNGPCLGYRKPNQPYKWISYKQVSDRAEYLGSCLLHKGYKPSQDQFIGIFAQNRPEWVISELACYTYSMVAVPLYDTLGAEAIIYVINRADISVVICDTPQKATMLIENVEKDLTPGLKTVILMDPFDDDLMKRGEKCGIEMLSLHDAENLGKENFKKPMPPNPEDLSVICFTSGTTGDPKGAMLTHQNIVSNMAAFLKFLEPIFQPTPEDVTISYLPLAHMFERLVQGVIFSCGGKIGFFQGDIRLLPDDMKALKPTVFPTVPRLLNRVYDKVQNEAKTPLKKFLLNLAIISKFNEVRNGIIRRNSLWDKLVFSKIQSSLGGKVRLMITGAAPISTPVLTFFRAAMGCWVFEAYGQTECTAGCSITSPGDWTAGHVGTPVSCNFVKLEDVADMNYFSVNNEGEICIKGNNVFKGYLKDPEKTQEVLDKDGWLHTGDIGRWLPNGTLKIIDRKKNIFKLAQGEYIAPEKIENVYSRSRPILQVFVHGESLRSFLIGVVVPDPESLPSFAAKIGVKGSFEELCQNQCVKKAILEDLQKVGKEGGLKSFEQVKSIFVHPEPFSIENGLLTPTLKAKRVELAKFFQTQIKSLYESIEE

Target ORF information:

RefSeq Version	NM_053607.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus acyl-CoA synthetase long-chain family member 5 (Acsl5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_053607.1

ORF Insert Sequence:

ATGCTTTTTA TTTTTAACTT CTTGTTTTCC CCACTTCCGA CCCCGGCCCT GATCTGCCTC 
CTGACATTTG GAACGGCTAT CTTCCTGTGG CTGATCAACA GACCTCAGCC AGTCTTACCT 
CTCATTGACT TGGACAACCA GTCTGTAGGG ATTGAGGGAG GAGCACGGAG AGGCGCTTTC 
CAGAAGAACA ATGACCTAAT CCTTTATTAC TTCTCAGACG CCAAGACGTT GTATGAAGTT 
TTCCAAAGAG GACTTGCTGT GTCTGACAAT GGACCTTGCT TGGGATACAG AAAGCCGAAC 
CAGCCCTACA AGTGGATATC CTACAAGCAG GTGTCTGATC GAGCAGAGTA CCTGGGCTCC 
TGTCTTTTGC ATAAAGGATA CAAGCCATCG CAGGACCAAT TTATTGGCAT CTTTGCTCAA 
AATAGGCCAG AGTGGGTCAT CTCCGAGTTA GCCTGTTACA CATATTCCAT GGTAGCTGTC 
CCCCTGTATG ACACACTGGG AGCAGAAGCC ATCATCTATG TCATCAACAG AGCTGATATC 
TCCGTGGTGA TCTGTGATAC ACCCCAAAAA GCAACAATGC TGATAGAAAA TGTGGAAAAG 
GACCTTACCC CAGGCCTGAA GACAGTCATC CTCATGGACC CTTTCGATGA TGACCTGATG 
AAACGAGGAG AGAAGTGTGG CATTGAGATG TTGTCTCTGC ATGATGCTGA GAATCTAGGC 
AAAGAGAACT TCAAAAAACC AATGCCTCCG AACCCAGAGG ATCTAAGTGT TATCTGTTTC 
ACCAGTGGAA CTACAGGTGA CCCCAAAGGA GCTATGCTGA CCCATCAAAA TATCGTTTCA 
AACATGGCTG CTTTCCTCAA ATTTCTGGAG CCTATCTTCC AGCCCACCCC CGAGGATGTG 
ACCATATCCT ACCTTCCCTT GGCCCATATG TTTGAGAGGC TTGTCCAGGG TGTCATATTT 
TCCTGTGGAG GCAAAATTGG GTTCTTCCAA GGAGATATCC GGTTGCTACC TGATGACATG 
AAGGCTTTGA AACCCACAGT GTTTCCCACA GTGCCTCGAC TCCTTAACAG GGTCTATGAT 
AAGGTACAAA ATGAAGCCAA GACACCTTTG AAAAAGTTCT TATTGAACTT GGCTATCATC 
AGCAAATTCA ACGAAGTGAG AAATGGCATC ATTCGGCGGA ACAGTTTGTG GGACAAGCTC 
GTGTTTTCAA AGATCCAAAG CAGCCTGGGT GGGAAGGTTC GTCTCATGAT CACTGGAGCC 
GCCCCTATCT CCACTCCAGT CTTGACGTTC TTCAGGGCTG CAATGGGATG CTGGGTGTTT 
GAAGCTTACG GCCAAACAGA ATGCACAGCT GGGTGTTCCA TTACATCACC TGGGGACTGG 
ACAGCAGGTC ATGTTGGGAC TCCGGTGTCC TGCAATTTTG TCAAGCTGGA AGATGTGGCT 
GACATGAACT ACTTTTCAGT AAACAACGAA GGCGAGATCT GCATCAAAGG CAACAATGTG 
TTCAAAGGCT ACCTAAAGGA CCCAGAGAAG ACACAGGAAG TACTGGACAA GGATGGCTGG 
CTTCACACTG GGGACATCGG TCGCTGGCTT CCAAATGGAA CTCTGAAAAT CATTGACCGG 
AAGAAGAATA TTTTCAAATT GGCACAAGGA GAATACATCG CTCCAGAGAA GATTGAAAAT 
GTTTATTCCA GGAGCAGACC AATATTGCAA GTTTTTGTCC ATGGGGAGAG CTTACGGTCA 
TTCCTGATAG GAGTGGTGGT TCCTGACCCA GAGTCACTTC CCTCATTTGC AGCCAAGATC 
GGGGTAAAGG GATCCTTTGA AGAACTATGC CAAAACCAGT GTGTAAAGAA AGCCATTTTA 
GAAGATTTGC AGAAAGTTGG GAAAGAAGGT GGCCTTAAAT CCTTTGAGCA GGTCAAGAGC 
ATCTTTGTGC ATCCGGAGCC GTTCTCAATT GAAAACGGGC TTCTGACACC GACACTGAAA 
GCCAAACGAG TGGAGCTTGC CAAGTTCTTC CAGACACAAA TCAAGAGCCT CTATGAGAGC 
ATCGAAGAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Acsl5 ( XM_017589808.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017445297.1 Rattus norvegicus acyl-CoA synthetase long-chain family member 5 (Acsl5), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa56884
Clone ID Related Accession (Same CDS sequence)	XM_017589808.1
Accession Version	XM_017589808.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2136bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	long-chain-fatty-acid--CoA ligase 5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 5% of CDS bases ##RefSeq-Attributes-END##

ATGCTTTTTA TTTTTAACTT CTTGTTTTCC CCACTTCCGA CCCCGGCCCT GATCTGCCTC 
CTGACATTTG GAACGGCTAT CTTCCTGTGG CTGATCAACA GACCTCAGCC AGTCTTACCT 
CTCATTGACT TGGACAACCA GTCTGTAGGG ATTGAGGGAG GAGCACGGAG AGGCGCTTTC 
CAGAAGAACA ATGACCTAAT CCTTTATTAC TTCTCAGACG CCAAGACGTT GTATGAAGTT 
TTCCAAAGAG GACTTGCTGT GTCTGACAAT GGACCTTGCT TGGGATACAG AAAGCCGAAC 
CAGCCCTACA AGTGGATATC CTACAAGCAG GTGTCTGATC GAGCAGAGTA CCTGGGCTCC 
TGTCTTTTGC ATAAAGGATA CAAGCCATCG CAGGACCAAT TTATTGGCAT CTTTGCTCAA 
AATAGGCCAG AGTGGGTCAT CTCCGAGTTA GCCTGTTACA CATATTCCAT GGTAGCTGTC 
CCCCTGTATG ACACACTGGG AGCAGAAGCC ATCATCTATG TCATCAACAG AGCTGATATC 
TCCGTGGTGA TCTGTGATAC ACCCCAAAAA GCAACAATGC TGATAGAAAA TGTGGAAAAG 
GACCTTACCC CAGGCCTGAA GACAGTCATC CTCATGGACC CTTTCGATGA TGACCTGATG 
AAACGAGGAG AGAAGTGTGG CATTGAGATG TTGTCTCTGC ATGATGCTGA GAATCTAGGC 
AAAGAGAACT TCAAAAAACC AGTGCCTCCG AACCCAGAGG ATCTAAGTGT TATCTGTTTC 
ACCAGTGGAA CTACAGGTGA CCCCAAAGGA GCTATGCTGA CCCATCAAAA TATCGTTTCA 
AACATGGCTG CTTTCCTCAA ATTTCTGGAG CCTATCTTCC AGCCCACACC CCAGAAAGTG 
GATGTGACCA CTATCATACC TTCCCTTGGG CCAATATGTT TGAGGGCTAT GTCAGGTGTA 
CCAGTGTCCT TGTCGTCTAG AGCAGATTTA GAAGGACCGA GGCACCGAGC CTTATCCTCC 
ATCTTCTTCC AGGGTGTCAT ATTTTCCTGT GGAGGCAAAA TTGGGTTCTT CCAAGGAGAT 
ATCCGGTTGC TACCTGATGA CATGAAGGCT TTGAAACCCA CAGTGTTTCC CACAGTGCCT 
CGACTCCTTA ACAGGGTCTA TGATAAGGTA CAAAATGAAG CCAAGACACC TTTGAAAAAG 
TTCTTATTGA ACTTGGCTAT CATCAGCAAA TTCAACGAAG TGAGAAATGG CATCATTCGG 
CGGAACAGTT TGTGGGACAA GCTCGTGTTT TCAAAGATCC AAAGCAGCCT GGGTGGGAAG 
GTTCGTCTCA TGATCACTGG AGCCGCCCCT ATCTCCACTC CAGTCTTGAC GTTCTTCAGG 
GCTGCAATGG GATGCTGGGT GTTTGAAGCT TACGGCCAAA CAGAATGCAC AGCTGGGTGT 
TCTATTACAT CACCTGGGGA CTGGACAGCA GGTCATGTTG GGACTCCGGT GTCCTGCAAT 
TTTGTCAAGC TGGAAGATGT GGCTGACATG AACTACTTTT CAGTAAACAA CGAAGGCGAG 
ATCTGCATCA AAGGCAACAA TGTGTTCAAA GGCTACCTAA AGGACCCAGA GAAGACACAG 
GAAGTACTGG ACAAGGATGG CTGGCTTCAC ACTGGGGACA TCGGTCGCTG GCTTCCAAAT 
GGAACTCTGA AAATCATTGA CCGGAAGAAG AATATTTTCA AATTGGCACA AGGAGAATAC 
ATCGCTCCAG AGAAGATTGA AAATGTTTAT TCCAGGAGCA GACCAATATT GCAAGTTTTT 
GTCCATGGGG AGAGCTTACG GTCATTCCTG ATAGGAGTGG TGGTTCCTGA CCCAGAGTCA 
CTTCCCTCAT TTGCAGCCAA GATCGGGGTA AAGGGATCCT TTGAAGAACT ATGCCAAAAC 
CAGTGTGTAA AGAAAGCCAT TTTAGAAGAT TTGCAGAAAG TTGGGAAAGA AGGTGGCCTT 
AAATCCTTTG AGCAGGTCAA GAGCATCTTT GTGCATCCGG AGCCGTTCTC AATTGAAAAC 
GGGCTTCTGA CACCGACACT GAAAGCCAAA CGAGTGGAGC TTGCCAAGTT CTTCCAGACA 
CAAATCAAGA GCCTCTATGA GAGCATCGAA GAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017445297.1
CDS	30..2165
Translation	MLFIFNFLFSPLPTPALICLLTFGTAIFLWLINRPQPVLPLIDLDNQSVGIEGGARRGAFQKNNDLILYYFSDAKTLYEVFQRGLAVSDNGPCLGYRKPNQPYKWISYKQVSDRAEYLGSCLLHKGYKPSQDQFIGIFAQNRPEWVISELACYTYSMVAVPLYDTLGAEAIIYVINRADISVVICDTPQKATMLIENVEKDLTPGLKTVILMDPFDDDLMKRGEKCGIEMLSLHDAENLGKENFKKPVPPNPEDLSVICFTSGTTGDPKGAMLTHQNIVSNMAAFLKFLEPIFQPTPQKVDVTTIIPSLGPICLRAMSGVPVSLSSRADLEGPRHRALSSIFFQGVIFSCGGKIGFFQGDIRLLPDDMKALKPTVFPTVPRLLNRVYDKVQNEAKTPLKKFLLNLAIISKFNEVRNGIIRRNSLWDKLVFSKIQSSLGGKVRLMITGAAPISTPVLTFFRAAMGCWVFEAYGQTECTAGCSITSPGDWTAGHVGTPVSCNFVKLEDVADMNYFSVNNEGEICIKGNNVFKGYLKDPEKTQEVLDKDGWLHTGDIGRWLPNGTLKIIDRKKNIFKLAQGEYIAPEKIENVYSRSRPILQVFVHGESLRSFLIGVVVPDPESLPSFAAKIGVKGSFEELCQNQCVKKAILEDLQKVGKEGGLKSFEQVKSIFVHPEPFSIENGLLTPTLKAKRVELAKFFQTQIKSLYESIEE

Target ORF information:

RefSeq Version	XM_017589808.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus acyl-CoA synthetase long-chain family member 5 (Acsl5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017589808.1

ORF Insert Sequence:

ATGCTTTTTA TTTTTAACTT CTTGTTTTCC CCACTTCCGA CCCCGGCCCT GATCTGCCTC 
CTGACATTTG GAACGGCTAT CTTCCTGTGG CTGATCAACA GACCTCAGCC AGTCTTACCT 
CTCATTGACT TGGACAACCA GTCTGTAGGG ATTGAGGGAG GAGCACGGAG AGGCGCTTTC 
CAGAAGAACA ATGACCTAAT CCTTTATTAC TTCTCAGACG CCAAGACGTT GTATGAAGTT 
TTCCAAAGAG GACTTGCTGT GTCTGACAAT GGACCTTGCT TGGGATACAG AAAGCCGAAC 
CAGCCCTACA AGTGGATATC CTACAAGCAG GTGTCTGATC GAGCAGAGTA CCTGGGCTCC 
TGTCTTTTGC ATAAAGGATA CAAGCCATCG CAGGACCAAT TTATTGGCAT CTTTGCTCAA 
AATAGGCCAG AGTGGGTCAT CTCCGAGTTA GCCTGTTACA CATATTCCAT GGTAGCTGTC 
CCCCTGTATG ACACACTGGG AGCAGAAGCC ATCATCTATG TCATCAACAG AGCTGATATC 
TCCGTGGTGA TCTGTGATAC ACCCCAAAAA GCAACAATGC TGATAGAAAA TGTGGAAAAG 
GACCTTACCC CAGGCCTGAA GACAGTCATC CTCATGGACC CTTTCGATGA TGACCTGATG 
AAACGAGGAG AGAAGTGTGG CATTGAGATG TTGTCTCTGC ATGATGCTGA GAATCTAGGC 
AAAGAGAACT TCAAAAAACC AGTGCCTCCG AACCCAGAGG ATCTAAGTGT TATCTGTTTC 
ACCAGTGGAA CTACAGGTGA CCCCAAAGGA GCTATGCTGA CCCATCAAAA TATCGTTTCA 
AACATGGCTG CTTTCCTCAA ATTTCTGGAG CCTATCTTCC AGCCCACACC CCAGAAAGTG 
GATGTGACCA CTATCATACC TTCCCTTGGG CCAATATGTT TGAGGGCTAT GTCAGGTGTA 
CCAGTGTCCT TGTCGTCTAG AGCAGATTTA GAAGGACCGA GGCACCGAGC CTTATCCTCC 
ATCTTCTTCC AGGGTGTCAT ATTTTCCTGT GGAGGCAAAA TTGGGTTCTT CCAAGGAGAT 
ATCCGGTTGC TACCTGATGA CATGAAGGCT TTGAAACCCA CAGTGTTTCC CACAGTGCCT 
CGACTCCTTA ACAGGGTCTA TGATAAGGTA CAAAATGAAG CCAAGACACC TTTGAAAAAG 
TTCTTATTGA ACTTGGCTAT CATCAGCAAA TTCAACGAAG TGAGAAATGG CATCATTCGG 
CGGAACAGTT TGTGGGACAA GCTCGTGTTT TCAAAGATCC AAAGCAGCCT GGGTGGGAAG 
GTTCGTCTCA TGATCACTGG AGCCGCCCCT ATCTCCACTC CAGTCTTGAC GTTCTTCAGG 
GCTGCAATGG GATGCTGGGT GTTTGAAGCT TACGGCCAAA CAGAATGCAC AGCTGGGTGT 
TCTATTACAT CACCTGGGGA CTGGACAGCA GGTCATGTTG GGACTCCGGT GTCCTGCAAT 
TTTGTCAAGC TGGAAGATGT GGCTGACATG AACTACTTTT CAGTAAACAA CGAAGGCGAG 
ATCTGCATCA AAGGCAACAA TGTGTTCAAA GGCTACCTAA AGGACCCAGA GAAGACACAG 
GAAGTACTGG ACAAGGATGG CTGGCTTCAC ACTGGGGACA TCGGTCGCTG GCTTCCAAAT 
GGAACTCTGA AAATCATTGA CCGGAAGAAG AATATTTTCA AATTGGCACA AGGAGAATAC 
ATCGCTCCAG AGAAGATTGA AAATGTTTAT TCCAGGAGCA GACCAATATT GCAAGTTTTT 
GTCCATGGGG AGAGCTTACG GTCATTCCTG ATAGGAGTGG TGGTTCCTGA CCCAGAGTCA 
CTTCCCTCAT TTGCAGCCAA GATCGGGGTA AAGGGATCCT TTGAAGAACT ATGCCAAAAC 
CAGTGTGTAA AGAAAGCCAT TTTAGAAGAT TTGCAGAAAG TTGGGAAAGA AGGTGGCCTT 
AAATCCTTTG AGCAGGTCAA GAGCATCTTT GTGCATCCGG AGCCGTTCTC AATTGAAAAC 
GGGCTTCTGA CACCGACACT GAAAGCCAAA CGAGTGGAGC TTGCCAAGTT CTTCCAGACA 
CAAATCAAGA GCCTCTATGA GAGCATCGAA GAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Long-chain acyl-CoA synthetase isoforms differ in preferences for eicosanoid species and long-chain fatty acids.
Journal of lipid research58(5)884-894(2017 May)
Klett EL,Chen S,Yechoor A,Lih FB,Coleman RA

Identification of acyl-CoA synthetases involved in the mammalian sphingosine 1-phosphate metabolic pathway.
Biochemical and biophysical research communications442(3-4)195-201(2013 Dec)
Ohkuni A,Ohno Y,Kihara A

The Sj?gren-Larsson syndrome gene encodes a hexadecenal dehydrogenase of the sphingosine 1-phosphate degradation pathway.
Molecular cell46(4)461-71(2012 May)
Nakahara K,Ohkuni A,Kitamura T,Abe K,Naganuma T,Ohno Y,Zoeller RA,Kihara A

Human intestinal acyl-CoA synthetase 5 is sensitive to the inhibitor triacsin C.
World journal of gastroenterology17(44)4883-9(2011 Nov)
Kaemmerer E,Peuscher A,Reinartz A,Liedtke C,Weiskirchen R,Kopitz J,Gassler N

Hepatic long-chain acyl-CoA synthetase 5 mediates fatty acid channeling between anabolic and catabolic pathways.
Journal of lipid research51(11)3270-80(2010 Nov)
Bu SY,Mashek DG

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Acsl5 cDNA ORF clone, Rattus norvegicus(Norway rat)

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Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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