Rps27 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Rps27
Entrez Gene ID	94266
Full Name	ribosomal protein S27
General protein information	Preferred Names ribosomal protein S27 Names 40S ribosomal protein S27
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 2 Map Location: 2q34
Summary	ribosomal protein subunit [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_053597.3	NP_446049.1	40S ribosomal protein S27

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Rps27	NP_446049.1
Danio rerio (zebrafish)	rps27.2	NP_957059.1
Drosophila melanogaster (fruit fly)	RpS27	NP_651359.1
Caenorhabditis elegans (roundworm)	rps-27	NP_503134.1
Saccharomyces cerevisiae (baker's yeast)	RPS27B	NP_011885.1
Magnaporthe oryzae (rice blast fungus)	MGG_02872	XP_003720862.1
Pan troglodytes (chimpanzee)	LOC100614845	XP_003312546.1
Saccharomyces cerevisiae (baker's yeast)	RPS27A	NP_012766.1
Xenopus tropicalis (tropical clawed frog)	rps27	NP_001165123.1
Homo sapiens (human)	RPS27	NP_001021.1
Pan troglodytes (chimpanzee)	LOC452745	XP_509802.2
Danio rerio (zebrafish)	rps27.1	NP_001153000.1
Kluyveromyces lactis	KLLA0E11661g	XP_454477.1
Schizosaccharomyces pombe (fission yeast)	rps27	NP_595214.1
Arabidopsis thaliana (thale cress)	RS27A	NP_191670.1
Arabidopsis thaliana (thale cress)	AT3G61111	NP_001118871.1
Oryza sativa (rice)	Os02g0478700	NP_001046847.1
Oryza sativa (rice)	Os04g0346100	NP_001052512.1
Pan troglodytes (chimpanzee)	RPS27	NP_001238926.1
Bos taurus (cattle)	RPS27	NP_001091604.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007157	XP_308611.1
Arabidopsis thaliana (thale cress)	AT5G47930	NP_199604.1
Canis lupus familiaris (dog)	RPS27	NP_001239215.1
Eremothecium gossypii	AGOS_ACR065C	NP_983467.2
Neurospora crassa	NCU00618	XP_965758.1
Arabidopsis thaliana (thale cress)	AT2G45710	NP_182095.1

Gene Ontology
Bibliography

Related articles in PubMed

Loss of function mutations in RPL27 and RPS27 identified by whole-exome sequencing in Diamond-Blackfan anaemia.
Wang R, Yoshida K, Toki T, Sawada T, Uechi T, Okuno Y, Sato-Otsubo A, Kudo K, Kamimaki I, Kanezaki R, Shiraishi Y, Chiba K, Tanaka H, Terui K, Sato T, Iribe Y, Ohga S, Kuramitsu M, Hamaguchi I, Ohara A, Hara J, Goi K, Matsubara K, Koike K, Ishiguro A, Okamoto Y, Watanabe K, Kanno H, Kojima S, Miyano S, Kenmochi N, Ogawa S, Ito E
British journal of haematology168(6)854-64(2015 Mar)

Structures of the human and Drosophila 80S ribosome.
Anger AM, Armache JP, Berninghausen O, Habeck M, Subklewe M, Wilson DN, Beckmann R
Nature497(7447)80-5(2013 May)

Molecular profiling of synaptic vesicle docking sites reveals novel proteins but few differences between glutamatergic and GABAergic synapses.
Boyken J, Gr?nborg M, Riedel D, Urlaub H, Jahn R, Chua JJ
Neuron78(2)285-97(2013 Apr)

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Baltz AG, Munschauer M, Schwanh?usser B, Vasile A, Murakawa Y, Schueler M, Youngs N, Penfold-Brown D, Drew K, Milek M, Wyler E, Bonneau R, Selbach M, Dieterich C, Landthaler M
Molecular cell46(5)674-90(2012 Jun)

Characterization of the proteome, diseases and evolution of the human postsynaptic density.
Bay?s A, van de Lagemaat LN, Collins MO, Croning MD, Whittle IR, Choudhary JS, Grant SG
Nature neuroscience14(1)19-21(2011 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Rps27 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Rps27 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa10022	NM_053597.3 Latest version!	Rattus norvegicus ribosomal protein S27 (Rps27), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Rps27 ( NM_053597.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_446049.1 Rattus norvegicus ribosomal protein S27 (Rps27), mRNA.

Price & Availability↑

CloneID	ORa10022
Clone ID Related Accession (Same CDS sequence)	NM_053597.3
Accession Version	NM_053597.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 255bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-03
Organism	Rattus norvegicus(Norway rat)
Product	40S ribosomal protein S27
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC061539.1. On Apr 14, 2007 this sequence version replaced NM_053597.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC061539.1, FQ133074.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCTCTCG CAAAGGATCT CCTTCATCCT TCTCCAGAAG AGGAGAAGAG GAAACACAAG 
AAAAAGCGCC TGGTGCAGAG CCCCAATTCC TACTTTATGG ATGTGAAATG CCCAGGATGC 
TATAAAATCA CCACGGTCTT TAGCCATGCA CAAACGGTAG TCTTGTGTGT TGGCTGCTCC 
ACTGTACTCT GTCAGCCTAC AGGTGGAAAA GCAAGGCTGA CAGAAGGATG CTCCTTCAGG 
AGGAAGCAGC ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_446049.1
CDS	34..288
Translation	MPLAKDLLHPSPEEEKRKHKKKRLVQSPNSYFMDVKCPGCYKITTVFSHAQTVVLCVGCSTVLCQPTGGKARLTEGCSFRRKQH

Target ORF information:

RefSeq Version	NM_053597.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ribosomal protein S27 (Rps27), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_053597.3

ORF Insert Sequence:

ATGCCTCTCG CAAAGGATCT CCTTCATCCT TCTCCAGAAG AGGAGAAGAG GAAACACAAG 
AAAAAGCGCC TGGTGCAGAG CCCCAATTCC TACTTTATGG ATGTGAAATG CCCAGGATGC 
TATAAAATCA CCACGGTCTT TAGCCATGCA CAAACGGTAG TCTTGTGTGT TGGCTGCTCC 
ACTGTACTCT GTCAGCCTAC AGGTGGAAAA GCAAGGCTGA CAGAAGGATG CTCCTTCAGG 
AGGAAGCAGC ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Loss of function mutations in RPL27 and RPS27 identified by whole-exome sequencing in Diamond-Blackfan anaemia.
British journal of haematology168(6)854-64(2015 Mar)
Wang R,Yoshida K,Toki T,Sawada T,Uechi T,Okuno Y,Sato-Otsubo A,Kudo K,Kamimaki I,Kanezaki R,Shiraishi Y,Chiba K,Tanaka H,Terui K,Sato T,Iribe Y,Ohga S,Kuramitsu M,Hamaguchi I,Ohara A,Hara J,Goi K,Matsubara K,Koike K,Ishiguro A,Okamoto Y,Watanabe K,Kanno H,Kojima S,Miyano S,Kenmochi N,Ogawa S,Ito E

Structures of the human and Drosophila 80S ribosome.
Nature497(7447)80-5(2013 May)
Anger AM,Armache JP,Berninghausen O,Habeck M,Subklewe M,Wilson DN,Beckmann R

Molecular profiling of synaptic vesicle docking sites reveals novel proteins but few differences between glutamatergic and GABAergic synapses.
Neuron78(2)285-97(2013 Apr)
Boyken J,Gr?nborg M,Riedel D,Urlaub H,Jahn R,Chua JJ

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Molecular cell46(5)674-90(2012 Jun)
Baltz AG,Munschauer M,Schwanh?usser B,Vasile A,Murakawa Y,Schueler M,Youngs N,Penfold-Brown D,Drew K,Milek M,Wyler E,Bonneau R,Selbach M,Dieterich C,Landthaler M

Characterization of the proteome, diseases and evolution of the human postsynaptic density.
Nature neuroscience14(1)19-21(2011 Jan)
Bay?s A,van de Lagemaat LN,Collins MO,Croning MD,Whittle IR,Choudhary JS,Grant SG

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Rps27 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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