Mbtps1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Mbtps1
Entrez Gene ID	89842
Full Name	membrane-bound transcription factor peptidase, site 1
Synonyms	S1p,Ski-1
General protein information	Preferred Names membrane-bound transcription factor peptidase, site 1 Names membrane-bound transcription factor site-1 protease S1P endopeptidase endopeptidase S1P membrane-bound transcription factor protease, site 1 site-1 protease subtilisin/kexin isozyme 1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 19 Map Location: 19q12
Summary	widely expressed Ca2+-dependent serine proteinase for cleavage of pro-brain-derived neurotrophic factor [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_017601395.1	XP_017456884.1	membrane-bound transcription factor site-1 protease isoform X2
XM_008772651.2	XP_008770873.1	membrane-bound transcription factor site-1 protease isoform X3
XM_006255723.3	XP_006255785.1	membrane-bound transcription factor site-1 protease isoform X1
NM_053569.1	NP_446021.1	membrane-bound transcription factor site-1 protease precursor

Pathways from BioSystems

Homologies

	MBTPS1	XP_001112150.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012203	XP_320328.4
Oryza sativa (rice)	Os06g0163500	NP_001056897.1
Bos taurus (cattle)	MBTPS1	NP_001020501.2
Homo sapiens (human)	MBTPS1	NP_003782.1
Canis lupus familiaris (dog)	MBTPS1	XP_850300.1
Gallus gallus (chicken)	MBTPS1	XP_003641945.1
Danio rerio (zebrafish)	mbtps1	NP_954683.1
Drosophila melanogaster (fruit fly)	S1P	NP_649337.2
Pan troglodytes (chimpanzee)	MBTPS1	XP_003315258.1
Arabidopsis thaliana (thale cress)	S1P	NP_197467.1
Xenopus tropicalis (tropical clawed frog)	mbtps1	NP_001072791.2
Mus musculus (house mouse)	Mbtps1	NP_062683.3
Rattus norvegicus (Norway rat)	Mbtps1	NP_446021.1

Gene Ontology
Bibliography

Related articles in PubMed

A key enzyme in the biogenesis of lysosomes is a protease that regulates cholesterol metabolism.
Marschner K, Kollmann K, Schweizer M, Braulke T, Pohl S
Science (New York, N.Y.)333(6038)87-90(2011 Jul)

Evolutionary conservation and adaptation in the mechanism that regulates SREBP action: what a long, strange tRIP it's been.
Osborne TF, Espenshade PJ
Genes & development23(22)2578-91(2009 Nov)

Sphingosine 1-phosphate accelerates wound healing in diabetic mice.
Kawanabe T, Kawakami T, Yatomi Y, Shimada S, Soma Y
Journal of dermatological science48(1)53-60(2007 Oct)

Tisp40, a spermatid specific bZip transcription factor, functions by binding to the unfolded protein response element via the Rip pathway.
Nagamori I, Yabuta N, Fujii T, Tanaka H, Yomogida K, Nishimune Y, Nojima H
Genes to cells : devoted to molecular & cellular mechanisms10(6)575-94(2005 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

S1P affects and sustains all key cellular processes responsible for wound repair and point to a unique potential for this molecule in the therapy of diabetic wounds, particularly as an angiogenic agent in treatment of diabetic wounds.
Title: Sphingosine 1-phosphate accelerates wound healing in diabetic mice.

The following Mbtps1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Mbtps1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa43894	XM_008772651.2 Latest version!	Rattus norvegicus membrane-bound transcription factor peptidase, site 1 (Mbtps1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
ORa59499	XM_017601395.1 Latest version!	Rattus norvegicus membrane-bound transcription factor peptidase, site 1 (Mbtps1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ORa12935	NM_053569.1 Latest version!	Rattus norvegicus membrane-bound transcription factor peptidase, site 1 (Mbtps1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
ORa38992	XM_006255723.3 Latest version!	Rattus norvegicus membrane-bound transcription factor peptidase, site 1 (Mbtps1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Mbtps1 ( XM_008772651.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008770873.1 Rattus norvegicus membrane-bound transcription factor peptidase, site 1 (Mbtps1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa43894
Clone ID Related Accession (Same CDS sequence)	XM_008772651.2
Accession Version	XM_008772651.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2703bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	membrane-bound transcription factor site-1 protease isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005118.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008772651.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCTCG TCAACATCTG GCTTCTTCTG CTGGTGGTTT TGCTCTGTGG GAAAAAGCAT 
CTGGGTGACA GGCTGGGGAA GAAAGCTTTT GAAAAGGCCC CATGCCCCAG CTGTTCCCAC 
CTGACTTTGA AGGTGGAATT CTCCTCAACT GTGGTGGAAT ATGAATATAT TGTGGCTTTC 
AACGGATACT TCACAGCCAA AGCTAGAAAC TCATTTATTT CAAGTGCTCT AAAAAGCAGT 
GAAGTGGACA ACTGGAGAAT AATACCTCGG AACAACCCAT CTAGTGACTA CCCTAGTGAT 
TTTGAGGTGA TTCAGATAAA AGAGAAGCAG AAGGCGGGGC TGCTCACACT TGAAGATCAC 
CCAAACATCA AGCGGGTGAC ACCCCAGCGG AAAGTCTTTC GTTCCCTGAA GTTTGCTGAA 
TCCGACCCCA TTGTGCCCTG TAATGAGACC CGGTGGAGCC AGAAGTGGCA GTCATCACGT 
CCCCTGAAAA GAGCCAGTCT CTCCCTGGGC TCTGGATTCT GGCATGCAAC AGGAAGGCAT 
TCAAGTCGAC GATTGCTGAG AGCCATTCCT CGCCAGGTTG CCCAGACATT GCAGGCAGAT 
GTGCTTTGGC AGATGGGATA CACAGGTGCT AATGTCAGGG TTGCTGTTTT TGATACTGGG 
CTCAGTGAGA AGCATCCACA TTTCAAGAAT GTGAAGGAAA GAACCAACTG GACCAATGAG 
CGGACCCTGG ACGATGGGCT GGGCCATGGC ACATTCGTTG CAGGTGTGAT TGCCAGCATG 
AGAGAGTGCC AAGGATTTGC CCCAGATGCA GAGCTGCACA TCTTCAGGGT CTTTACCAAC 
AATCAGGTGT CTTACACGTC TTGGTTTTTG GATGCCTTCA ACTATGCCAT CCTAAAGAAG 
ATTGACGTTC TGAACCTTAG CATCGGCGGG CCTGACTTCA TGGATCACCC CTTTGTTGAC 
AAGGTATGGG AATTAACAGC GAACAATGTA ATCATGGTTT CTGCTATTGG CAATGATGGA 
CCTCTCTATG GCACTCTGAA TAACCCTGCT GATCAGATGG ATGTGATTGG AGTGGGTGGC 
ATTGACTTTG AAGACAACAT CGCCCGCTTC TCTTCCAGGG GAATGACTAC CTGGGAACTA 
CCGGGAGGCT ATGGTCGTGT GAAGCCTGAC ATTGTCACCT ATGGTGCTGG AGTGCGGGGT 
TCTGGTGTGA AAGGGGGCTG CCGTGCACTC TCAGGGACCA GTGTCGCCTC CCCAGTGGTT 
GCTGGGGCTG TCACCTTGTT AGTAAGCACA GTACAGAAGC GGGAGCTAGT GAATCCTGCC 
AGTGTGAAGC AAGCTTTGAT AGCATCAGCC CGGAGACTTC CTGGTGTCAA CATGTTTGAG 
CAAGGCCATG GCAAGTTGGA TCTACTGCGA GCCTATCAGA TCCTCAGCAG CTATAAACCG 
CAGGCGAGCC TGAGTCCTAG CTACATCGAC CTGACTGAGT GTCCCTACAT GTGGCCCTAC 
TGCTCCCAGC CCATCTACTA TGGAGGAATG CCAACAATTG TTAATGTCAC CATCCTCAAT 
GGCATGGGAG TTACAGGAAG AATTGTGGAT AAGCCTGAGT GGCGACCCTA TTTACCACAG 
AATGGAGACA ACATTGAAGT GGCCTTCTCC TACTCCTCAG TGTTGTGGCC TTGGTCAGGT 
TACCTTGCCA TCTCCATTTC TGTGACCAAG AAGGCAGCTT CCTGGGAAGG CATCGCGCAG 
GGCCACATCA TGATCACAGT GGCTTCCCCA GCAGAGACGG AATTAAAAAA TGGTGCCGAG 
CATACTTCCA CAGTGAAGCT GCCCATCAAG GTGAAGATCA TTCCCACCCC TCCTCGGAGC 
AAGAGAGTCC TCTGGGACCA GTACCACAAC CTCCGCTACC CACCCGGCTA CTTCCCCAGG 
GACAACTTGC GGATGAAGAA TGATCCTTTA GACTGGAATG GCGACCACGT CCACACCAAC 
TTCAGGGACA TGTACCAGCA TCTGCGCAGC ATGGGCTACT TTGTGGAGGT GCTTGGTGCC 
CCATTCACAT GCTTTGACGC CACGCAGTAC GGCACTCTGC TTATGGTGGA CAGTGAGGAA 
GAGTACTTCC CTGAGGAGAT TGCTAAGCTG AGGAGGGACG TGGACAATGG CCTTTCCCTT 
GTCGTCTTCA GTGACTGGTA CAACACTTCT GTTATGAGAA AAGTGAAGTT TTACGATGAA 
AACACAAGGC AGTGGTGGAT GCCAGATACT GGAGGAGCCA ACGTCCCAGC TCTAAACGAG 
CTGCTGTCTG TGTGGAACAT GGGGTTCAGT GACGGCCTGT ATGAAGGGGA GTTTGCCCTG 
GCAAACCACG ACATGTACTA TGCATCGGGG TGCAGCATTG CCAGGTTTCC AGAAGATGGT 
GTGGTGATCA CACAGACTTT CAAGGACCAA GGATTGGAAG TCTTAAAACA AGAGACAGCA 
GTTGTCGACA ATGTCCCCAT TCTGGGGCTT TATCAGATTC CAGCTGAAGG TGGAGGCCGG 
ATTGTGCTGT ATGGAGACTC CAACTGCTTG GATGACAGTC ACAGACAGAA GGCCTGTGGC 
TTGACCAGTG AAAATGGTCG AAAGGCTCAC TTGACATCAT GTGAGTATGT GACGGACGGC 
TTGTTGGGCA CGCGGCTGGG GGCATGGGGG TGGGCTGGGG GAAGCAGCCC CAGCCCCTCT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008770873.1
CDS	1152..3854
Translation	MKLVNIWLLLLVVLLCGKKHLGDRLGKKAFEKAPCPSCSHLTLKVEFSSTVVEYEYIVAFNGYFTAKARNSFISSALKSSEVDNWRIIPRNNPSSDYPSDFEVIQIKEKQKAGLLTLEDHPNIKRVTPQRKVFRSLKFAESDPIVPCNETRWSQKWQSSRPLKRASLSLGSGFWHATGRHSSRRLLRAIPRQVAQTLQADVLWQMGYTGANVRVAVFDTGLSEKHPHFKNVKERTNWTNERTLDDGLGHGTFVAGVIASMRECQGFAPDAELHIFRVFTNNQVSYTSWFLDAFNYAILKKIDVLNLSIGGPDFMDHPFVDKVWELTANNVIMVSAIGNDGPLYGTLNNPADQMDVIGVGGIDFEDNIARFSSRGMTTWELPGGYGRVKPDIVTYGAGVRGSGVKGGCRALSGTSVASPVVAGAVTLLVSTVQKRELVNPASVKQALIASARRLPGVNMFEQGHGKLDLLRAYQILSSYKPQASLSPSYIDLTECPYMWPYCSQPIYYGGMPTIVNVTILNGMGVTGRIVDKPEWRPYLPQNGDNIEVAFSYSSVLWPWSGYLAISISVTKKAASWEGIAQGHIMITVASPAETELKNGAEHTSTVKLPIKVKIIPTPPRSKRVLWDQYHNLRYPPGYFPRDNLRMKNDPLDWNGDHVHTNFRDMYQHLRSMGYFVEVLGAPFTCFDATQYGTLLMVDSEEEYFPEEIAKLRRDVDNGLSLVVFSDWYNTSVMRKVKFYDENTRQWWMPDTGGANVPALNELLSVWNMGFSDGLYEGEFALANHDMYYASGCSIARFPEDGVVITQTFKDQGLEVLKQETAVVDNVPILGLYQIPAEGGGRIVLYGDSNCLDDSHRQKACGLTSENGRKAHLTSCEYVTDGLLGTRLGAWGWAGGSSPSPS

Target ORF information:

RefSeq Version	XM_008772651.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus membrane-bound transcription factor peptidase, site 1 (Mbtps1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008772651.2

ORF Insert Sequence:

ATGAAGCTCG TCAACATCTG GCTTCTTCTG CTGGTGGTTT TGCTCTGTGG GAAAAAGCAT 
CTGGGTGACA GGCTGGGGAA GAAAGCTTTT GAAAAGGCCC CATGCCCCAG CTGTTCCCAC 
CTGACTTTGA AGGTGGAATT CTCCTCAACT GTGGTGGAAT ATGAATATAT TGTGGCTTTC 
AACGGATACT TCACAGCCAA AGCTAGAAAC TCATTTATTT CAAGTGCTCT AAAAAGCAGT 
GAAGTGGACA ACTGGAGAAT AATACCTCGG AACAACCCAT CTAGTGACTA CCCTAGTGAT 
TTTGAGGTGA TTCAGATAAA AGAGAAGCAG AAGGCGGGGC TGCTCACACT TGAAGATCAC 
CCAAACATCA AGCGGGTGAC ACCCCAGCGG AAAGTCTTTC GTTCCCTGAA GTTTGCTGAA 
TCCGACCCCA TTGTGCCCTG TAATGAGACC CGGTGGAGCC AGAAGTGGCA GTCATCACGT 
CCCCTGAAAA GAGCCAGTCT CTCCCTGGGC TCTGGATTCT GGCATGCAAC AGGAAGGCAT 
TCAAGTCGAC GATTGCTGAG AGCCATTCCT CGCCAGGTTG CCCAGACATT GCAGGCAGAT 
GTGCTTTGGC AGATGGGATA CACAGGTGCT AATGTCAGGG TTGCTGTTTT TGATACTGGG 
CTCAGTGAGA AGCATCCACA TTTCAAGAAT GTGAAGGAAA GAACCAACTG GACCAATGAG 
CGGACCCTGG ACGATGGGCT GGGCCATGGC ACATTCGTTG CAGGTGTGAT TGCCAGCATG 
AGAGAGTGCC AAGGATTTGC CCCAGATGCA GAGCTGCACA TCTTCAGGGT CTTTACCAAC 
AATCAGGTGT CTTACACGTC TTGGTTTTTG GATGCCTTCA ACTATGCCAT CCTAAAGAAG 
ATTGACGTTC TGAACCTTAG CATCGGCGGG CCTGACTTCA TGGATCACCC CTTTGTTGAC 
AAGGTATGGG AATTAACAGC GAACAATGTA ATCATGGTTT CTGCTATTGG CAATGATGGA 
CCTCTCTATG GCACTCTGAA TAACCCTGCT GATCAGATGG ATGTGATTGG AGTGGGTGGC 
ATTGACTTTG AAGACAACAT CGCCCGCTTC TCTTCCAGGG GAATGACTAC CTGGGAACTA 
CCGGGAGGCT ATGGTCGTGT GAAGCCTGAC ATTGTCACCT ATGGTGCTGG AGTGCGGGGT 
TCTGGTGTGA AAGGGGGCTG CCGTGCACTC TCAGGGACCA GTGTCGCCTC CCCAGTGGTT 
GCTGGGGCTG TCACCTTGTT AGTAAGCACA GTACAGAAGC GGGAGCTAGT GAATCCTGCC 
AGTGTGAAGC AAGCTTTGAT AGCATCAGCC CGGAGACTTC CTGGTGTCAA CATGTTTGAG 
CAAGGCCATG GCAAGTTGGA TCTACTGCGA GCCTATCAGA TCCTCAGCAG CTATAAACCG 
CAGGCGAGCC TGAGTCCTAG CTACATCGAC CTGACTGAGT GTCCCTACAT GTGGCCCTAC 
TGCTCCCAGC CCATCTACTA TGGAGGAATG CCAACAATTG TTAATGTCAC CATCCTCAAT 
GGCATGGGAG TTACAGGAAG AATTGTGGAT AAGCCTGAGT GGCGACCCTA TTTACCACAG 
AATGGAGACA ACATTGAAGT GGCCTTCTCC TACTCCTCAG TGTTGTGGCC TTGGTCAGGT 
TACCTTGCCA TCTCCATTTC TGTGACCAAG AAGGCAGCTT CCTGGGAAGG CATCGCGCAG 
GGCCACATCA TGATCACAGT GGCTTCCCCA GCAGAGACGG AATTAAAAAA TGGTGCCGAG 
CATACTTCCA CAGTGAAGCT GCCCATCAAG GTGAAGATCA TTCCCACCCC TCCTCGGAGC 
AAGAGAGTCC TCTGGGACCA GTACCACAAC CTCCGCTACC CACCCGGCTA CTTCCCCAGG 
GACAACTTGC GGATGAAGAA TGATCCTTTA GACTGGAATG GCGACCACGT CCACACCAAC 
TTCAGGGACA TGTACCAGCA TCTGCGCAGC ATGGGCTACT TTGTGGAGGT GCTTGGTGCC 
CCATTCACAT GCTTTGACGC CACGCAGTAC GGCACTCTGC TTATGGTGGA CAGTGAGGAA 
GAGTACTTCC CTGAGGAGAT TGCTAAGCTG AGGAGGGACG TGGACAATGG CCTTTCCCTT 
GTCGTCTTCA GTGACTGGTA CAACACTTCT GTTATGAGAA AAGTGAAGTT TTACGATGAA 
AACACAAGGC AGTGGTGGAT GCCAGATACT GGAGGAGCCA ACGTCCCAGC TCTAAACGAG 
CTGCTGTCTG TGTGGAACAT GGGGTTCAGT GACGGCCTGT ATGAAGGGGA GTTTGCCCTG 
GCAAACCACG ACATGTACTA TGCATCGGGG TGCAGCATTG CCAGGTTTCC AGAAGATGGT 
GTGGTGATCA CACAGACTTT CAAGGACCAA GGATTGGAAG TCTTAAAACA AGAGACAGCA 
GTTGTCGACA ATGTCCCCAT TCTGGGGCTT TATCAGATTC CAGCTGAAGG TGGAGGCCGG 
ATTGTGCTGT ATGGAGACTC CAACTGCTTG GATGACAGTC ACAGACAGAA GGCCTGTGGC 
TTGACCAGTG AAAATGGTCG AAAGGCTCAC TTGACATCAT GTGAGTATGT GACGGACGGC 
TTGTTGGGCA CGCGGCTGGG GGCATGGGGG TGGGCTGGGG GAAGCAGCCC CAGCCCCTCT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mbtps1 ( XM_017601395.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017456884.1 Rattus norvegicus membrane-bound transcription factor peptidase, site 1 (Mbtps1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa59499
Clone ID Related Accession (Same CDS sequence)	XM_017601395.1
Accession Version	XM_017601395.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3027bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	membrane-bound transcription factor site-1 protease isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005118.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCTCG TCAACATCTG GCTTCTTCTG CTGGTGGTTT TGCTCTGTGG GAAAAAGCAT 
CTGGGTGACA GGCTGGGGAA GAAAGCTTTT GAAAAGGCCC CATGCCCCAG CTGTTCCCAC 
CTGACTTTGA AGGTGGAATT CTCCTCAACT GTGGTGGAAT ATGAATATAT TGTGGCTTTC 
AACGGATACT TCACAGCCAA AGCTAGAAAC TCATTTATTT CAAGTGCTCT AAAAAGCAGT 
GAAGTGGACA ACTGGAGAAT AATACCTCGG AACAACCCAT CTAGTGACTA CCCTAGTGAT 
TTTGAGGTGA TTCAGATAAA AGAGAAGCAG AAGGCGGGGC TGCTCACACT TGAAGATCAC 
CCAAACATCA AGCGGGTGAC ACCCCAGCGG AAAGTCTTTC GTTCCCTGAA GTTTGCTGAA 
TCCGACCCCA TTGTGCCCTG TAATGAGACC CGGTGGAGCC AGAAGTGGCA GTCATCACGT 
CCCCTGAAAA GAGCCAGTCT CTCCCTGGGC TCTGGATTCT GGCATGCAAC AGGAAGGCAT 
TCAAGTCGAC GATTGCTGAG AGCCATTCCT CGCCAGGTTG CCCAGACATT GCAGGCAGAT 
GTGCTTTGGC AGATGGGATA CACAGGTGCT AATGTCAGGG TTGCTGTTTT TGATACTGGG 
CTCAGTGAGA AGCATCCACA TTTCAAGAAT GTGAAGGAAA GAACCAACTG GACCAATGAG 
CGGACCCTGG ACGATGGGCT GGGCCATGGC ACATTCGTTG CAGGTGTGAT TGCCAGCATG 
AGAGAGTGCC AAGGATTTGC CCCAGATGCA GAGCTGCACA TCTTCAGGGT CTTTACCAAC 
AATCAGGTGT CTTACACGTC TTGGTTTTTG GATGCCTTCA ACTATGCCAT CCTAAAGAAG 
ATTGACGTTC TGAACCTTAG CATCGGCGGG CCTGACTTCA TGGATCACCC CTTTGTTGAC 
AAGGTATGGG AATTAACAGC GAACAATGTA ATCATGGTTT CTGCTATTGG CAATGATGGA 
CCTCTCTATG GCACTCTGAA TAACCCTGCT GATCAGATGG ATGTGATTGG AGTGGGTGGC 
ATTGACTTTG AAGACAACAT CGCCCGCTTC TCTTCCAGGG GAATGACTAC CTGGGAACTA 
CCGGGAGGCT ATGGTCGTGT GAAGCCTGAC ATTGTCACCT ATGGTGCTGG AGTGCGGGGT 
TCTGGTGTGA AAGGGGGCTG CCGTGCACTC TCAGGGACCA GTGTCGCCTC CCCAGTGGTT 
GCTGGGGCTG TCACCTTGTT AGTAAGCACA GTACAGAAGC GGGAGCTAGT GAATCCTGCC 
AGTGTGAAGC AAGCTTTGAT AGCATCAGCC CGGAGACTTC CTGGTGTCAA CATGTTTGAG 
CAAGGCCATG GCAAGTTGGA TCTACTGCGA GCCTATCAGA TCCTCAGCAG CTATAAACCG 
CAGGCGAGCC TGAGTCCTAG CTACATCGAC CTGACTGAGT GTCCCTACAT GTGGCCCTAC 
TGCTCCCAGC CCATCTACTA TGGAGGAATG CCAACAATTG TTAATGTCAC CATCCTCAAT 
GGCATGGGAG TTACAGGAAG AATTGTGGAT AAGCCTGAGT GGCGACCCTA TTTACCACAG 
AATGGAGACA ACATTGAAGT GGCCTTCTCC TACTCCTCAG TGTTGTGGCC TTGGTCAGGT 
TACCTTGCCA TCTCCATTTC TGTGACCAAG AAGGCAGCTT CCTGGGAAGG CATCGCGCAG 
GGCCACATCA TGATCACAGT GGCTTCCCCA GCAGAGACGG AATTAAAAAA TGGTGCCGAG 
CATACTTCCA CAGTGAAGCT GCCCATCAAG GTGAAGATCA TTCCCACCCC TCCTCGGAGC 
AAGAGAGTCC TCTGGGACCA GTACCACAAC CTCCGCTACC CACCCGGCTA CTTCCCCAGG 
GACAACTTGC GGATGAAGAA TGATCCTTTA GACTGGAATG GCGACCACGT CCACACCAAC 
TTCAGGGACA TGTACCAGCA TCTGCGCAGC ATGGGCTACT TTGTGGAGGT GCTTGGTGCC 
CCATTCACAT GCTTTGACGC CACGCAGTAC GGCACTCTGC TTATGGTGGA CAGTGAGGAA 
GAGTACTTCC CTGAGGAGAT TGCTAAGCTG AGGAGGGACG TGGACAATGG CCTTTCCCTT 
GTCGTCTTCA GTGACTGGTA CAACACTTCT GTTATGAGAA AAGTGAAGTT TTACGATGAA 
AACACAAGGC AGTGGTGGAT GCCAGATACT GGAGGAGCCA ACGTCCCAGC TCTAAACGAG 
CTGCTGTCTG TGTGGAACAT GGGGTTCAGT GACGGCCTGT ATGAAGGGGA GTTTGCCCTG 
GCAAACCACG ACATGTACTA TGCATCGGGG TGCAGCATTG CCAGGTTTCC AGAAGATGGT 
GTGGTGATCA CACAGACTTT CAAGGACCAA GGATTGGAAG TCTTAAAACA AGAGACAGCA 
GTTGTCGACA ATGTCCCCAT TCTGGGGCTT TATCAGATTC CAGCTGAAGG TGGAGGCCGG 
ATTGTGCTGT ATGGAGACTC CAACTGCTTG GATGACAGTC ACAGACAGAA GGGAAACCAC 
CTTCATCGCT ACTCCAAAGT TCTTGAGGCC CACTTGGGAG ACCCGAAACC TCGGCCCCTT 
CCAGCCTGTC CACACTTGTC GTGGGCCAAG CCACAGCCTT TGAATGAGAC GGCGCCCAGT 
AATCTTTGGA AACACCAGAA GCTGCTCTCC ATTGACCTGG ACAAAGTAGT GTTACCCAAC 
TTTCGCTCAA ATCGCCCTCA AGTGAGACCT TTGTCCCCTG GAGAAAGTGG TGCCTGGGAC 
ATTCCTGGAG GGATCATGCC TGGCCGCTAC AACCAGGAAG TAGGCCAGAC CATCCCTGTT 
TTTGCCTTCC TTGGAGCCAT GGTGGCCCTG GCCTTCTTCG TGGTACAGAT CAGTAAGGCC 
AAGAGCCGGC CGAAGCGGAG GAGGCCCAGG GCAAAGCGTC CACAACTTGC ACAGCAGGCC 
CACCCTGCAA GGACCCCGTC AGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017456884.1
CDS	1150..4176
Translation	MKLVNIWLLLLVVLLCGKKHLGDRLGKKAFEKAPCPSCSHLTLKVEFSSTVVEYEYIVAFNGYFTAKARNSFISSALKSSEVDNWRIIPRNNPSSDYPSDFEVIQIKEKQKAGLLTLEDHPNIKRVTPQRKVFRSLKFAESDPIVPCNETRWSQKWQSSRPLKRASLSLGSGFWHATGRHSSRRLLRAIPRQVAQTLQADVLWQMGYTGANVRVAVFDTGLSEKHPHFKNVKERTNWTNERTLDDGLGHGTFVAGVIASMRECQGFAPDAELHIFRVFTNNQVSYTSWFLDAFNYAILKKIDVLNLSIGGPDFMDHPFVDKVWELTANNVIMVSAIGNDGPLYGTLNNPADQMDVIGVGGIDFEDNIARFSSRGMTTWELPGGYGRVKPDIVTYGAGVRGSGVKGGCRALSGTSVASPVVAGAVTLLVSTVQKRELVNPASVKQALIASARRLPGVNMFEQGHGKLDLLRAYQILSSYKPQASLSPSYIDLTECPYMWPYCSQPIYYGGMPTIVNVTILNGMGVTGRIVDKPEWRPYLPQNGDNIEVAFSYSSVLWPWSGYLAISISVTKKAASWEGIAQGHIMITVASPAETELKNGAEHTSTVKLPIKVKIIPTPPRSKRVLWDQYHNLRYPPGYFPRDNLRMKNDPLDWNGDHVHTNFRDMYQHLRSMGYFVEVLGAPFTCFDATQYGTLLMVDSEEEYFPEEIAKLRRDVDNGLSLVVFSDWYNTSVMRKVKFYDENTRQWWMPDTGGANVPALNELLSVWNMGFSDGLYEGEFALANHDMYYASGCSIARFPEDGVVITQTFKDQGLEVLKQETAVVDNVPILGLYQIPAEGGGRIVLYGDSNCLDDSHRQKGNHLHRYSKVLEAHLGDPKPRPLPACPHLSWAKPQPLNETAPSNLWKHQKLLSIDLDKVVLPNFRSNRPQVRPLSPGESGAWDIPGGIMPGRYNQEVGQTIPVFAFLGAMVALAFFVVQISKAKSRPKRRRPRAKRPQLAQQAHPARTPSV

Target ORF information:

RefSeq Version	XM_017601395.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus membrane-bound transcription factor peptidase, site 1 (Mbtps1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017601395.1

ORF Insert Sequence:

ATGAAGCTCG TCAACATCTG GCTTCTTCTG CTGGTGGTTT TGCTCTGTGG GAAAAAGCAT 
CTGGGTGACA GGCTGGGGAA GAAAGCTTTT GAAAAGGCCC CATGCCCCAG CTGTTCCCAC 
CTGACTTTGA AGGTGGAATT CTCCTCAACT GTGGTGGAAT ATGAATATAT TGTGGCTTTC 
AACGGATACT TCACAGCCAA AGCTAGAAAC TCATTTATTT CAAGTGCTCT AAAAAGCAGT 
GAAGTGGACA ACTGGAGAAT AATACCTCGG AACAACCCAT CTAGTGACTA CCCTAGTGAT 
TTTGAGGTGA TTCAGATAAA AGAGAAGCAG AAGGCGGGGC TGCTCACACT TGAAGATCAC 
CCAAACATCA AGCGGGTGAC ACCCCAGCGG AAAGTCTTTC GTTCCCTGAA GTTTGCTGAA 
TCCGACCCCA TTGTGCCCTG TAATGAGACC CGGTGGAGCC AGAAGTGGCA GTCATCACGT 
CCCCTGAAAA GAGCCAGTCT CTCCCTGGGC TCTGGATTCT GGCATGCAAC AGGAAGGCAT 
TCAAGTCGAC GATTGCTGAG AGCCATTCCT CGCCAGGTTG CCCAGACATT GCAGGCAGAT 
GTGCTTTGGC AGATGGGATA CACAGGTGCT AATGTCAGGG TTGCTGTTTT TGATACTGGG 
CTCAGTGAGA AGCATCCACA TTTCAAGAAT GTGAAGGAAA GAACCAACTG GACCAATGAG 
CGGACCCTGG ACGATGGGCT GGGCCATGGC ACATTCGTTG CAGGTGTGAT TGCCAGCATG 
AGAGAGTGCC AAGGATTTGC CCCAGATGCA GAGCTGCACA TCTTCAGGGT CTTTACCAAC 
AATCAGGTGT CTTACACGTC TTGGTTTTTG GATGCCTTCA ACTATGCCAT CCTAAAGAAG 
ATTGACGTTC TGAACCTTAG CATCGGCGGG CCTGACTTCA TGGATCACCC CTTTGTTGAC 
AAGGTATGGG AATTAACAGC GAACAATGTA ATCATGGTTT CTGCTATTGG CAATGATGGA 
CCTCTCTATG GCACTCTGAA TAACCCTGCT GATCAGATGG ATGTGATTGG AGTGGGTGGC 
ATTGACTTTG AAGACAACAT CGCCCGCTTC TCTTCCAGGG GAATGACTAC CTGGGAACTA 
CCGGGAGGCT ATGGTCGTGT GAAGCCTGAC ATTGTCACCT ATGGTGCTGG AGTGCGGGGT 
TCTGGTGTGA AAGGGGGCTG CCGTGCACTC TCAGGGACCA GTGTCGCCTC CCCAGTGGTT 
GCTGGGGCTG TCACCTTGTT AGTAAGCACA GTACAGAAGC GGGAGCTAGT GAATCCTGCC 
AGTGTGAAGC AAGCTTTGAT AGCATCAGCC CGGAGACTTC CTGGTGTCAA CATGTTTGAG 
CAAGGCCATG GCAAGTTGGA TCTACTGCGA GCCTATCAGA TCCTCAGCAG CTATAAACCG 
CAGGCGAGCC TGAGTCCTAG CTACATCGAC CTGACTGAGT GTCCCTACAT GTGGCCCTAC 
TGCTCCCAGC CCATCTACTA TGGAGGAATG CCAACAATTG TTAATGTCAC CATCCTCAAT 
GGCATGGGAG TTACAGGAAG AATTGTGGAT AAGCCTGAGT GGCGACCCTA TTTACCACAG 
AATGGAGACA ACATTGAAGT GGCCTTCTCC TACTCCTCAG TGTTGTGGCC TTGGTCAGGT 
TACCTTGCCA TCTCCATTTC TGTGACCAAG AAGGCAGCTT CCTGGGAAGG CATCGCGCAG 
GGCCACATCA TGATCACAGT GGCTTCCCCA GCAGAGACGG AATTAAAAAA TGGTGCCGAG 
CATACTTCCA CAGTGAAGCT GCCCATCAAG GTGAAGATCA TTCCCACCCC TCCTCGGAGC 
AAGAGAGTCC TCTGGGACCA GTACCACAAC CTCCGCTACC CACCCGGCTA CTTCCCCAGG 
GACAACTTGC GGATGAAGAA TGATCCTTTA GACTGGAATG GCGACCACGT CCACACCAAC 
TTCAGGGACA TGTACCAGCA TCTGCGCAGC ATGGGCTACT TTGTGGAGGT GCTTGGTGCC 
CCATTCACAT GCTTTGACGC CACGCAGTAC GGCACTCTGC TTATGGTGGA CAGTGAGGAA 
GAGTACTTCC CTGAGGAGAT TGCTAAGCTG AGGAGGGACG TGGACAATGG CCTTTCCCTT 
GTCGTCTTCA GTGACTGGTA CAACACTTCT GTTATGAGAA AAGTGAAGTT TTACGATGAA 
AACACAAGGC AGTGGTGGAT GCCAGATACT GGAGGAGCCA ACGTCCCAGC TCTAAACGAG 
CTGCTGTCTG TGTGGAACAT GGGGTTCAGT GACGGCCTGT ATGAAGGGGA GTTTGCCCTG 
GCAAACCACG ACATGTACTA TGCATCGGGG TGCAGCATTG CCAGGTTTCC AGAAGATGGT 
GTGGTGATCA CACAGACTTT CAAGGACCAA GGATTGGAAG TCTTAAAACA AGAGACAGCA 
GTTGTCGACA ATGTCCCCAT TCTGGGGCTT TATCAGATTC CAGCTGAAGG TGGAGGCCGG 
ATTGTGCTGT ATGGAGACTC CAACTGCTTG GATGACAGTC ACAGACAGAA GGGAAACCAC 
CTTCATCGCT ACTCCAAAGT TCTTGAGGCC CACTTGGGAG ACCCGAAACC TCGGCCCCTT 
CCAGCCTGTC CACACTTGTC GTGGGCCAAG CCACAGCCTT TGAATGAGAC GGCGCCCAGT 
AATCTTTGGA AACACCAGAA GCTGCTCTCC ATTGACCTGG ACAAAGTAGT GTTACCCAAC 
TTTCGCTCAA ATCGCCCTCA AGTGAGACCT TTGTCCCCTG GAGAAAGTGG TGCCTGGGAC 
ATTCCTGGAG GGATCATGCC TGGCCGCTAC AACCAGGAAG TAGGCCAGAC CATCCCTGTT 
TTTGCCTTCC TTGGAGCCAT GGTGGCCCTG GCCTTCTTCG TGGTACAGAT CAGTAAGGCC 
AAGAGCCGGC CGAAGCGGAG GAGGCCCAGG GCAAAGCGTC CACAACTTGC ACAGCAGGCC 
CACCCTGCAA GGACCCCGTC AGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mbtps1 ( NM_053569.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_446021.1 Rattus norvegicus membrane-bound transcription factor peptidase, site 1 (Mbtps1), mRNA.

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CloneID	ORa12935
Clone ID Related Accession (Same CDS sequence)	NM_053569.1
Accession Version	NM_053569.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3159bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-14
Organism	Rattus norvegicus(Norway rat)
Product	membrane-bound transcription factor site-1 protease precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF094821.1. ##Evidence-Data-START## Transcript exon combination :: AF094821.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGAAGCTCG TCAACATCTG GCTTCTTCTG CTGGTGGTTT TGCTCTGTGG GAAAAAGCAT 
CTGGGTGACA GGCTGGGGAA GAAAGCTTTT GAAAAGGCCC CATGCCCCAG CTGTTCCCAC 
CTGACTTTGA AGGTGGAATT CTCCTCAACT GTGGTGGAAT ATGAATATAT TGTGGCTTTC 
AACGGATACT TCACAGCCAA AGCTAGAAAC TCATTTATTT CAAGTGCTCT AAAAAGCAGT 
GAAGTGGACA ACTGGAGAAT AATACCTCGG AACAACCCAT CTAGTGACTA CCCTAGTGAT 
TTTGAGGTGA TTCAGATAAA AGAGAAGCAG AAGGCGGGGC TGCTCACACT TGAAGATCAC 
CCAAACATCA AGCGGGTGAC ACCCCAGCGG AAAGTCTTTC GTTCCCTGAA GTTTGCTGAA 
TCCGACCCCA TTGTGCCCTG TAATGAGACC CGGTGGAGCC AGAAGTGGCA GTCATCACGT 
CCCCTGAAAA GAGCCAGTCT CTCCCTGGGC TCTGGATTCT GGCATGCAAC AGGAAGGCAT 
TCAAGTCGAC GATTGCTGAG AGCCATTCCT CGCCAGGTTG CCCAGACATT GCAGGCAGAT 
GTGCTTTGGC AGATGGGATA CACAGGTGCT AATGTCAGGG TTGCCGTTTT TGATACTGGG 
CTCAGTGAGA AGCATCCACA TTTCAAGAAT GTGAAGGAAA GAACCAACTG GACCAATGAG 
CGGACCCTGG ACGATGGGCT GGGCCATGGC ACATTCGTTG CAGGTGTGAT TGCCAGCATG 
AGAGAGTGCC AAGGATTTGC CCCAGATGCA GAGCTGCACA TCTTCAGGGT CTTTACCAAC 
AATCAGGTGT CTTACACGTC TTGGTTTTTG GATGCCTTCA ACTATGCCAT CCTAAAGAAG 
ATGGACGTTC TGAACCTTAG CATCGGTGGG CCTGACTTCA TGGATCACCC CTTTGTTGAC 
AAGGTATGGG AATTAACAGC GAACAATGTA ATCATGGTTT CTGCTATTGG CAATGATGGA 
CCTCTCTATG GCACTCTGAA TAACCCTGCT GATCAGATGG ATGTGATTGG AGTGGGTGGC 
ATTGACTTTG AAGACAACAT CGCCCGCTTC TCTTCCAGGG GAATGACTAC CTGGGAACTA 
CCGGGAGGCT ATGGTCGTGT GAAGCCTGAC ATTGTCACCT ATGGTGCTGG AGTGCGGGGT 
TCTGGTGTGA AAGGGGGCTG CCGTGCACTC TCAGGGACCA GTGTCGCCTC CCCAGTGGTT 
GCTGGGGCTG TCACCTTGTT AGTAAGCACA GTACAGAAGC GGGAGCTAGT GAATCCTGCC 
AGTGTGAAGC AAGCTTTGAT AGCATCAGCC CGGAGACTTC CTGGTGTCAA CATGTTTGAG 
CAAGGCCATG GCAAGTTGGA TCTACTGCGA GCCTATCAGA TCCTCAGCAG CTATAAACCG 
CAGGCGAGCC TGAGTCCTAG CTACATCGAC CTGACTGAGT GTCCCTACAT GTGGCCCTAC 
TGCTCCCAGC CCATCTACTA TGGAGGAATG CCAACAATTG TTAATGTCAC CATCCTCAAT 
GGCATGGGAG TTACAGGAAG AATTGTGGAT AAGCCTGAGT GGCGACCCTA TTTACCACAG 
AATGGAGACA ACATTGAAGT GGCCTTCTCC TACTCCTCAG TGTTGTGGCC TTGGTCAGGT 
TACCTTGCCA TCTCCATTTC TGTGACCAAG AAGGCAGCTT CCTGGGAAGG CATCGCGCAG 
GGCCACATCA TGATCACAGT GGCTTCCCCA GCAGAGACGG AATTAAAAAA TGGTGCCGAG 
CATACTTCCA CAGTGAAGCT GCCCATCAAG GTGAAGATCA TTCCCACCCC TCCTCGGAGC 
AAGAGAGTCC TCTGGGACCA GTACCACAAC CTCCGCTACC CACCCGGCTA CTTCCCCAGG 
GACAACTTGC GGATGAAGAA TGATCCTTTA GACTGGAATG GCGACCACGT CCACACCAAC 
TTCAGGGACA TGTACCAGCA TCTGCGCAGC ATGGGCTACT TTGTGGAGGT GCTTGGTGCC 
CCATTCACAT GCTTTGACGC CACGCAGTAC GGCACTCTGC TTATGGTGGA CAGTGAGGAA 
GAGTACTTCC CTGAGGAGAT TGCTAAGCTG AGGAGGGACG TGGACAATGG CCTTTCCCTT 
GTCGTCTTCA GTGACTGGTA CAACACTTCT GTTATGAGAA AAGTGAAGTT TTACGATGAA 
AACACAAGGC AGTGGTGGAT GCCAGATACT GGAGGAGCCA ACGTCCCAGC TCTAAACGAG 
CTGCTGTCTG TGTGGAACAT GGGGTTCAGT GACGGCCTGT ATGAAGGGGA GTTTGCCCTG 
GCAAACCACG ACATGTACTA TGCATCGGGG TGCAGCATTG CCAGGTTTCC AGAAGATGGT 
GTGGTGATCA CACAGACTTT CAAGGACCAA GGATTGGAAG TCTTAAAACA AGAGACAGCA 
GTTGTCGACA ATGTCCCCAT TCTGGGGCTA TATCAGATTC CAGCTGAAGG TGGAGGCCGG 
ATTGTGCTGT ATGGAGACTC CAACTGCTTG GATGACAGTC ACAGACAGAA GGACTGCTTT 
TGGCTTCTGG ATGCACTCCT TCAGTACACA TCCTATGGTG TGACCCCTCC CAGCCTCAGC 
CATTCAGGGA ACCGGCAGCG CCCACCCAGC GGGGCTGGCT TGGCCCCTCC TGAAAGGATG 
GAAGGAAACC ACCTTCATCG CTACTCCAAA GTTCTTGAGG CCCACTTGGG AGACCCGAAA 
CCTCGGCCCC TTCCAGCCTG TCCACACTTG TCGTGGGCCA AGCCACAGCC TTTGAATGAG 
ACGGCACCCA GTAATCTTTG GAAACACCAG AAGCTGCTCT CCATTGACCT GGACAAAGTA 
GTGTTACCCA ACTTTCGCTC AAATCGCCCT CAAGTGAGAC CTTTGTCCCC TGGAGAAAGT 
GGTGCCTGGG ACATTCCTGG AGGGATCATG CCTGGCCGCT ACAACCAGGA AGTAGGCCAG 
ACCATCCCTG TTTTTGCCTT CCTTGGAGCC ATGGTGGCCC TGGCCTTCTT CGTGGTACAG 
ATCAGTAAGG CCAAGAGCCG GCCGAAGCGG AGGAGGCCCA GGGCAAAGCG TCCACAACTT 
GCACAGCAGG CCCACCCTGC AAGGACCCCG TCAGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_446021.1
CDS	418..3576
Translation	MKLVNIWLLLLVVLLCGKKHLGDRLGKKAFEKAPCPSCSHLTLKVEFSSTVVEYEYIVAFNGYFTAKARNSFISSALKSSEVDNWRIIPRNNPSSDYPSDFEVIQIKEKQKAGLLTLEDHPNIKRVTPQRKVFRSLKFAESDPIVPCNETRWSQKWQSSRPLKRASLSLGSGFWHATGRHSSRRLLRAIPRQVAQTLQADVLWQMGYTGANVRVAVFDTGLSEKHPHFKNVKERTNWTNERTLDDGLGHGTFVAGVIASMRECQGFAPDAELHIFRVFTNNQVSYTSWFLDAFNYAILKKMDVLNLSIGGPDFMDHPFVDKVWELTANNVIMVSAIGNDGPLYGTLNNPADQMDVIGVGGIDFEDNIARFSSRGMTTWELPGGYGRVKPDIVTYGAGVRGSGVKGGCRALSGTSVASPVVAGAVTLLVSTVQKRELVNPASVKQALIASARRLPGVNMFEQGHGKLDLLRAYQILSSYKPQASLSPSYIDLTECPYMWPYCSQPIYYGGMPTIVNVTILNGMGVTGRIVDKPEWRPYLPQNGDNIEVAFSYSSVLWPWSGYLAISISVTKKAASWEGIAQGHIMITVASPAETELKNGAEHTSTVKLPIKVKIIPTPPRSKRVLWDQYHNLRYPPGYFPRDNLRMKNDPLDWNGDHVHTNFRDMYQHLRSMGYFVEVLGAPFTCFDATQYGTLLMVDSEEEYFPEEIAKLRRDVDNGLSLVVFSDWYNTSVMRKVKFYDENTRQWWMPDTGGANVPALNELLSVWNMGFSDGLYEGEFALANHDMYYASGCSIARFPEDGVVITQTFKDQGLEVLKQETAVVDNVPILGLYQIPAEGGGRIVLYGDSNCLDDSHRQKDCFWLLDALLQYTSYGVTPPSLSHSGNRQRPPSGAGLAPPERMEGNHLHRYSKVLEAHLGDPKPRPLPACPHLSWAKPQPLNETAPSNLWKHQKLLSIDLDKVVLPNFRSNRPQVRPLSPGESGAWDIPGGIMPGRYNQEVGQTIPVFAFLGAMVALAFFVVQISKAKSRPKRRRPRAKRPQLAQQAHPARTPSV

Target ORF information:

RefSeq Version	NM_053569.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus membrane-bound transcription factor peptidase, site 1 (Mbtps1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_053569.1

ORF Insert Sequence:

ATGAAGCTCG TCAACATCTG GCTTCTTCTG CTGGTGGTTT TGCTCTGTGG GAAAAAGCAT 
CTGGGTGACA GGCTGGGGAA GAAAGCTTTT GAAAAGGCCC CATGCCCCAG CTGTTCCCAC 
CTGACTTTGA AGGTGGAATT CTCCTCAACT GTGGTGGAAT ATGAATATAT TGTGGCTTTC 
AACGGATACT TCACAGCCAA AGCTAGAAAC TCATTTATTT CAAGTGCTCT AAAAAGCAGT 
GAAGTGGACA ACTGGAGAAT AATACCTCGG AACAACCCAT CTAGTGACTA CCCTAGTGAT 
TTTGAGGTGA TTCAGATAAA AGAGAAGCAG AAGGCGGGGC TGCTCACACT TGAAGATCAC 
CCAAACATCA AGCGGGTGAC ACCCCAGCGG AAAGTCTTTC GTTCCCTGAA GTTTGCTGAA 
TCCGACCCCA TTGTGCCCTG TAATGAGACC CGGTGGAGCC AGAAGTGGCA GTCATCACGT 
CCCCTGAAAA GAGCCAGTCT CTCCCTGGGC TCTGGATTCT GGCATGCAAC AGGAAGGCAT 
TCAAGTCGAC GATTGCTGAG AGCCATTCCT CGCCAGGTTG CCCAGACATT GCAGGCAGAT 
GTGCTTTGGC AGATGGGATA CACAGGTGCT AATGTCAGGG TTGCCGTTTT TGATACTGGG 
CTCAGTGAGA AGCATCCACA TTTCAAGAAT GTGAAGGAAA GAACCAACTG GACCAATGAG 
CGGACCCTGG ACGATGGGCT GGGCCATGGC ACATTCGTTG CAGGTGTGAT TGCCAGCATG 
AGAGAGTGCC AAGGATTTGC CCCAGATGCA GAGCTGCACA TCTTCAGGGT CTTTACCAAC 
AATCAGGTGT CTTACACGTC TTGGTTTTTG GATGCCTTCA ACTATGCCAT CCTAAAGAAG 
ATGGACGTTC TGAACCTTAG CATCGGTGGG CCTGACTTCA TGGATCACCC CTTTGTTGAC 
AAGGTATGGG AATTAACAGC GAACAATGTA ATCATGGTTT CTGCTATTGG CAATGATGGA 
CCTCTCTATG GCACTCTGAA TAACCCTGCT GATCAGATGG ATGTGATTGG AGTGGGTGGC 
ATTGACTTTG AAGACAACAT CGCCCGCTTC TCTTCCAGGG GAATGACTAC CTGGGAACTA 
CCGGGAGGCT ATGGTCGTGT GAAGCCTGAC ATTGTCACCT ATGGTGCTGG AGTGCGGGGT 
TCTGGTGTGA AAGGGGGCTG CCGTGCACTC TCAGGGACCA GTGTCGCCTC CCCAGTGGTT 
GCTGGGGCTG TCACCTTGTT AGTAAGCACA GTACAGAAGC GGGAGCTAGT GAATCCTGCC 
AGTGTGAAGC AAGCTTTGAT AGCATCAGCC CGGAGACTTC CTGGTGTCAA CATGTTTGAG 
CAAGGCCATG GCAAGTTGGA TCTACTGCGA GCCTATCAGA TCCTCAGCAG CTATAAACCG 
CAGGCGAGCC TGAGTCCTAG CTACATCGAC CTGACTGAGT GTCCCTACAT GTGGCCCTAC 
TGCTCCCAGC CCATCTACTA TGGAGGAATG CCAACAATTG TTAATGTCAC CATCCTCAAT 
GGCATGGGAG TTACAGGAAG AATTGTGGAT AAGCCTGAGT GGCGACCCTA TTTACCACAG 
AATGGAGACA ACATTGAAGT GGCCTTCTCC TACTCCTCAG TGTTGTGGCC TTGGTCAGGT 
TACCTTGCCA TCTCCATTTC TGTGACCAAG AAGGCAGCTT CCTGGGAAGG CATCGCGCAG 
GGCCACATCA TGATCACAGT GGCTTCCCCA GCAGAGACGG AATTAAAAAA TGGTGCCGAG 
CATACTTCCA CAGTGAAGCT GCCCATCAAG GTGAAGATCA TTCCCACCCC TCCTCGGAGC 
AAGAGAGTCC TCTGGGACCA GTACCACAAC CTCCGCTACC CACCCGGCTA CTTCCCCAGG 
GACAACTTGC GGATGAAGAA TGATCCTTTA GACTGGAATG GCGACCACGT CCACACCAAC 
TTCAGGGACA TGTACCAGCA TCTGCGCAGC ATGGGCTACT TTGTGGAGGT GCTTGGTGCC 
CCATTCACAT GCTTTGACGC CACGCAGTAC GGCACTCTGC TTATGGTGGA CAGTGAGGAA 
GAGTACTTCC CTGAGGAGAT TGCTAAGCTG AGGAGGGACG TGGACAATGG CCTTTCCCTT 
GTCGTCTTCA GTGACTGGTA CAACACTTCT GTTATGAGAA AAGTGAAGTT TTACGATGAA 
AACACAAGGC AGTGGTGGAT GCCAGATACT GGAGGAGCCA ACGTCCCAGC TCTAAACGAG 
CTGCTGTCTG TGTGGAACAT GGGGTTCAGT GACGGCCTGT ATGAAGGGGA GTTTGCCCTG 
GCAAACCACG ACATGTACTA TGCATCGGGG TGCAGCATTG CCAGGTTTCC AGAAGATGGT 
GTGGTGATCA CACAGACTTT CAAGGACCAA GGATTGGAAG TCTTAAAACA AGAGACAGCA 
GTTGTCGACA ATGTCCCCAT TCTGGGGCTA TATCAGATTC CAGCTGAAGG TGGAGGCCGG 
ATTGTGCTGT ATGGAGACTC CAACTGCTTG GATGACAGTC ACAGACAGAA GGACTGCTTT 
TGGCTTCTGG ATGCACTCCT TCAGTACACA TCCTATGGTG TGACCCCTCC CAGCCTCAGC 
CATTCAGGGA ACCGGCAGCG CCCACCCAGC GGGGCTGGCT TGGCCCCTCC TGAAAGGATG 
GAAGGAAACC ACCTTCATCG CTACTCCAAA GTTCTTGAGG CCCACTTGGG AGACCCGAAA 
CCTCGGCCCC TTCCAGCCTG TCCACACTTG TCGTGGGCCA AGCCACAGCC TTTGAATGAG 
ACGGCACCCA GTAATCTTTG GAAACACCAG AAGCTGCTCT CCATTGACCT GGACAAAGTA 
GTGTTACCCA ACTTTCGCTC AAATCGCCCT CAAGTGAGAC CTTTGTCCCC TGGAGAAAGT 
GGTGCCTGGG ACATTCCTGG AGGGATCATG CCTGGCCGCT ACAACCAGGA AGTAGGCCAG 
ACCATCCCTG TTTTTGCCTT CCTTGGAGCC ATGGTGGCCC TGGCCTTCTT CGTGGTACAG 
ATCAGTAAGG CCAAGAGCCG GCCGAAGCGG AGGAGGCCCA GGGCAAAGCG TCCACAACTT 
GCACAGCAGG CCCACCCTGC AAGGACCCCG TCAGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mbtps1 ( XM_006255723.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006255785.1 Rattus norvegicus membrane-bound transcription factor peptidase, site 1 (Mbtps1), transcript variant X1, mRNA.

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CloneID	ORa38992
Clone ID Related Accession (Same CDS sequence)	XM_006255723.3
Accession Version	XM_006255723.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3159bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	membrane-bound transcription factor site-1 protease isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005118.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006255723.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCTCG TCAACATCTG GCTTCTTCTG CTGGTGGTTT TGCTCTGTGG GAAAAAGCAT 
CTGGGTGACA GGCTGGGGAA GAAAGCTTTT GAAAAGGCCC CATGCCCCAG CTGTTCCCAC 
CTGACTTTGA AGGTGGAATT CTCCTCAACT GTGGTGGAAT ATGAATATAT TGTGGCTTTC 
AACGGATACT TCACAGCCAA AGCTAGAAAC TCATTTATTT CAAGTGCTCT AAAAAGCAGT 
GAAGTGGACA ACTGGAGAAT AATACCTCGG AACAACCCAT CTAGTGACTA CCCTAGTGAT 
TTTGAGGTGA TTCAGATAAA AGAGAAGCAG AAGGCGGGGC TGCTCACACT TGAAGATCAC 
CCAAACATCA AGCGGGTGAC ACCCCAGCGG AAAGTCTTTC GTTCCCTGAA GTTTGCTGAA 
TCCGACCCCA TTGTGCCCTG TAATGAGACC CGGTGGAGCC AGAAGTGGCA GTCATCACGT 
CCCCTGAAAA GAGCCAGTCT CTCCCTGGGC TCTGGATTCT GGCATGCAAC AGGAAGGCAT 
TCAAGTCGAC GATTGCTGAG AGCCATTCCT CGCCAGGTTG CCCAGACATT GCAGGCAGAT 
GTGCTTTGGC AGATGGGATA CACAGGTGCT AATGTCAGGG TTGCTGTTTT TGATACTGGG 
CTCAGTGAGA AGCATCCACA TTTCAAGAAT GTGAAGGAAA GAACCAACTG GACCAATGAG 
CGGACCCTGG ACGATGGGCT GGGCCATGGC ACATTCGTTG CAGGTGTGAT TGCCAGCATG 
AGAGAGTGCC AAGGATTTGC CCCAGATGCA GAGCTGCACA TCTTCAGGGT CTTTACCAAC 
AATCAGGTGT CTTACACGTC TTGGTTTTTG GATGCCTTCA ACTATGCCAT CCTAAAGAAG 
ATTGACGTTC TGAACCTTAG CATCGGCGGG CCTGACTTCA TGGATCACCC CTTTGTTGAC 
AAGGTATGGG AATTAACAGC GAACAATGTA ATCATGGTTT CTGCTATTGG CAATGATGGA 
CCTCTCTATG GCACTCTGAA TAACCCTGCT GATCAGATGG ATGTGATTGG AGTGGGTGGC 
ATTGACTTTG AAGACAACAT CGCCCGCTTC TCTTCCAGGG GAATGACTAC CTGGGAACTA 
CCGGGAGGCT ATGGTCGTGT GAAGCCTGAC ATTGTCACCT ATGGTGCTGG AGTGCGGGGT 
TCTGGTGTGA AAGGGGGCTG CCGTGCACTC TCAGGGACCA GTGTCGCCTC CCCAGTGGTT 
GCTGGGGCTG TCACCTTGTT AGTAAGCACA GTACAGAAGC GGGAGCTAGT GAATCCTGCC 
AGTGTGAAGC AAGCTTTGAT AGCATCAGCC CGGAGACTTC CTGGTGTCAA CATGTTTGAG 
CAAGGCCATG GCAAGTTGGA TCTACTGCGA GCCTATCAGA TCCTCAGCAG CTATAAACCG 
CAGGCGAGCC TGAGTCCTAG CTACATCGAC CTGACTGAGT GTCCCTACAT GTGGCCCTAC 
TGCTCCCAGC CCATCTACTA TGGAGGAATG CCAACAATTG TTAATGTCAC CATCCTCAAT 
GGCATGGGAG TTACAGGAAG AATTGTGGAT AAGCCTGAGT GGCGACCCTA TTTACCACAG 
AATGGAGACA ACATTGAAGT GGCCTTCTCC TACTCCTCAG TGTTGTGGCC TTGGTCAGGT 
TACCTTGCCA TCTCCATTTC TGTGACCAAG AAGGCAGCTT CCTGGGAAGG CATCGCGCAG 
GGCCACATCA TGATCACAGT GGCTTCCCCA GCAGAGACGG AATTAAAAAA TGGTGCCGAG 
CATACTTCCA CAGTGAAGCT GCCCATCAAG GTGAAGATCA TTCCCACCCC TCCTCGGAGC 
AAGAGAGTCC TCTGGGACCA GTACCACAAC CTCCGCTACC CACCCGGCTA CTTCCCCAGG 
GACAACTTGC GGATGAAGAA TGATCCTTTA GACTGGAATG GCGACCACGT CCACACCAAC 
TTCAGGGACA TGTACCAGCA TCTGCGCAGC ATGGGCTACT TTGTGGAGGT GCTTGGTGCC 
CCATTCACAT GCTTTGACGC CACGCAGTAC GGCACTCTGC TTATGGTGGA CAGTGAGGAA 
GAGTACTTCC CTGAGGAGAT TGCTAAGCTG AGGAGGGACG TGGACAATGG CCTTTCCCTT 
GTCGTCTTCA GTGACTGGTA CAACACTTCT GTTATGAGAA AAGTGAAGTT TTACGATGAA 
AACACAAGGC AGTGGTGGAT GCCAGATACT GGAGGAGCCA ACGTCCCAGC TCTAAACGAG 
CTGCTGTCTG TGTGGAACAT GGGGTTCAGT GACGGCCTGT ATGAAGGGGA GTTTGCCCTG 
GCAAACCACG ACATGTACTA TGCATCGGGG TGCAGCATTG CCAGGTTTCC AGAAGATGGT 
GTGGTGATCA CACAGACTTT CAAGGACCAA GGATTGGAAG TCTTAAAACA AGAGACAGCA 
GTTGTCGACA ATGTCCCCAT TCTGGGGCTT TATCAGATTC CAGCTGAAGG TGGAGGCCGG 
ATTGTGCTGT ATGGAGACTC CAACTGCTTG GATGACAGTC ACAGACAGAA GGACTGCTTT 
TGGCTTCTGG ATGCACTCCT TCAGTACACA TCCTATGGTG TGACCCCTCC CAGCCTCAGC 
CATTCAGGGA ACCGGCAGCG CCCACCCAGC GGGGCTGGCT TGGCCCCTCC TGAAAGGATG 
GAAGGAAACC ACCTTCATCG CTACTCCAAA GTTCTTGAGG CCCACTTGGG AGACCCGAAA 
CCTCGGCCCC TTCCAGCCTG TCCACACTTG TCGTGGGCCA AGCCACAGCC TTTGAATGAG 
ACGGCGCCCA GTAATCTTTG GAAACACCAG AAGCTGCTCT CCATTGACCT GGACAAAGTA 
GTGTTACCCA ACTTTCGCTC AAATCGCCCT CAAGTGAGAC CTTTGTCCCC TGGAGAAAGT 
GGTGCCTGGG ACATTCCTGG AGGGATCATG CCTGGCCGCT ACAACCAGGA AGTAGGCCAG 
ACCATCCCTG TTTTTGCCTT CCTTGGAGCC ATGGTGGCCC TGGCCTTCTT CGTGGTACAG 
ATCAGTAAGG CCAAGAGCCG GCCGAAGCGG AGGAGGCCCA GGGCAAAGCG TCCACAACTT 
GCACAGCAGG CCCACCCTGC AAGGACCCCG TCAGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006255785.1
CDS	1149..4307
Translation	MKLVNIWLLLLVVLLCGKKHLGDRLGKKAFEKAPCPSCSHLTLKVEFSSTVVEYEYIVAFNGYFTAKARNSFISSALKSSEVDNWRIIPRNNPSSDYPSDFEVIQIKEKQKAGLLTLEDHPNIKRVTPQRKVFRSLKFAESDPIVPCNETRWSQKWQSSRPLKRASLSLGSGFWHATGRHSSRRLLRAIPRQVAQTLQADVLWQMGYTGANVRVAVFDTGLSEKHPHFKNVKERTNWTNERTLDDGLGHGTFVAGVIASMRECQGFAPDAELHIFRVFTNNQVSYTSWFLDAFNYAILKKIDVLNLSIGGPDFMDHPFVDKVWELTANNVIMVSAIGNDGPLYGTLNNPADQMDVIGVGGIDFEDNIARFSSRGMTTWELPGGYGRVKPDIVTYGAGVRGSGVKGGCRALSGTSVASPVVAGAVTLLVSTVQKRELVNPASVKQALIASARRLPGVNMFEQGHGKLDLLRAYQILSSYKPQASLSPSYIDLTECPYMWPYCSQPIYYGGMPTIVNVTILNGMGVTGRIVDKPEWRPYLPQNGDNIEVAFSYSSVLWPWSGYLAISISVTKKAASWEGIAQGHIMITVASPAETELKNGAEHTSTVKLPIKVKIIPTPPRSKRVLWDQYHNLRYPPGYFPRDNLRMKNDPLDWNGDHVHTNFRDMYQHLRSMGYFVEVLGAPFTCFDATQYGTLLMVDSEEEYFPEEIAKLRRDVDNGLSLVVFSDWYNTSVMRKVKFYDENTRQWWMPDTGGANVPALNELLSVWNMGFSDGLYEGEFALANHDMYYASGCSIARFPEDGVVITQTFKDQGLEVLKQETAVVDNVPILGLYQIPAEGGGRIVLYGDSNCLDDSHRQKDCFWLLDALLQYTSYGVTPPSLSHSGNRQRPPSGAGLAPPERMEGNHLHRYSKVLEAHLGDPKPRPLPACPHLSWAKPQPLNETAPSNLWKHQKLLSIDLDKVVLPNFRSNRPQVRPLSPGESGAWDIPGGIMPGRYNQEVGQTIPVFAFLGAMVALAFFVVQISKAKSRPKRRRPRAKRPQLAQQAHPARTPSV

Target ORF information:

RefSeq Version	XM_006255723.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus membrane-bound transcription factor peptidase, site 1 (Mbtps1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006255723.3

ORF Insert Sequence:

ATGAAGCTCG TCAACATCTG GCTTCTTCTG CTGGTGGTTT TGCTCTGTGG GAAAAAGCAT 
CTGGGTGACA GGCTGGGGAA GAAAGCTTTT GAAAAGGCCC CATGCCCCAG CTGTTCCCAC 
CTGACTTTGA AGGTGGAATT CTCCTCAACT GTGGTGGAAT ATGAATATAT TGTGGCTTTC 
AACGGATACT TCACAGCCAA AGCTAGAAAC TCATTTATTT CAAGTGCTCT AAAAAGCAGT 
GAAGTGGACA ACTGGAGAAT AATACCTCGG AACAACCCAT CTAGTGACTA CCCTAGTGAT 
TTTGAGGTGA TTCAGATAAA AGAGAAGCAG AAGGCGGGGC TGCTCACACT TGAAGATCAC 
CCAAACATCA AGCGGGTGAC ACCCCAGCGG AAAGTCTTTC GTTCCCTGAA GTTTGCTGAA 
TCCGACCCCA TTGTGCCCTG TAATGAGACC CGGTGGAGCC AGAAGTGGCA GTCATCACGT 
CCCCTGAAAA GAGCCAGTCT CTCCCTGGGC TCTGGATTCT GGCATGCAAC AGGAAGGCAT 
TCAAGTCGAC GATTGCTGAG AGCCATTCCT CGCCAGGTTG CCCAGACATT GCAGGCAGAT 
GTGCTTTGGC AGATGGGATA CACAGGTGCT AATGTCAGGG TTGCTGTTTT TGATACTGGG 
CTCAGTGAGA AGCATCCACA TTTCAAGAAT GTGAAGGAAA GAACCAACTG GACCAATGAG 
CGGACCCTGG ACGATGGGCT GGGCCATGGC ACATTCGTTG CAGGTGTGAT TGCCAGCATG 
AGAGAGTGCC AAGGATTTGC CCCAGATGCA GAGCTGCACA TCTTCAGGGT CTTTACCAAC 
AATCAGGTGT CTTACACGTC TTGGTTTTTG GATGCCTTCA ACTATGCCAT CCTAAAGAAG 
ATTGACGTTC TGAACCTTAG CATCGGCGGG CCTGACTTCA TGGATCACCC CTTTGTTGAC 
AAGGTATGGG AATTAACAGC GAACAATGTA ATCATGGTTT CTGCTATTGG CAATGATGGA 
CCTCTCTATG GCACTCTGAA TAACCCTGCT GATCAGATGG ATGTGATTGG AGTGGGTGGC 
ATTGACTTTG AAGACAACAT CGCCCGCTTC TCTTCCAGGG GAATGACTAC CTGGGAACTA 
CCGGGAGGCT ATGGTCGTGT GAAGCCTGAC ATTGTCACCT ATGGTGCTGG AGTGCGGGGT 
TCTGGTGTGA AAGGGGGCTG CCGTGCACTC TCAGGGACCA GTGTCGCCTC CCCAGTGGTT 
GCTGGGGCTG TCACCTTGTT AGTAAGCACA GTACAGAAGC GGGAGCTAGT GAATCCTGCC 
AGTGTGAAGC AAGCTTTGAT AGCATCAGCC CGGAGACTTC CTGGTGTCAA CATGTTTGAG 
CAAGGCCATG GCAAGTTGGA TCTACTGCGA GCCTATCAGA TCCTCAGCAG CTATAAACCG 
CAGGCGAGCC TGAGTCCTAG CTACATCGAC CTGACTGAGT GTCCCTACAT GTGGCCCTAC 
TGCTCCCAGC CCATCTACTA TGGAGGAATG CCAACAATTG TTAATGTCAC CATCCTCAAT 
GGCATGGGAG TTACAGGAAG AATTGTGGAT AAGCCTGAGT GGCGACCCTA TTTACCACAG 
AATGGAGACA ACATTGAAGT GGCCTTCTCC TACTCCTCAG TGTTGTGGCC TTGGTCAGGT 
TACCTTGCCA TCTCCATTTC TGTGACCAAG AAGGCAGCTT CCTGGGAAGG CATCGCGCAG 
GGCCACATCA TGATCACAGT GGCTTCCCCA GCAGAGACGG AATTAAAAAA TGGTGCCGAG 
CATACTTCCA CAGTGAAGCT GCCCATCAAG GTGAAGATCA TTCCCACCCC TCCTCGGAGC 
AAGAGAGTCC TCTGGGACCA GTACCACAAC CTCCGCTACC CACCCGGCTA CTTCCCCAGG 
GACAACTTGC GGATGAAGAA TGATCCTTTA GACTGGAATG GCGACCACGT CCACACCAAC 
TTCAGGGACA TGTACCAGCA TCTGCGCAGC ATGGGCTACT TTGTGGAGGT GCTTGGTGCC 
CCATTCACAT GCTTTGACGC CACGCAGTAC GGCACTCTGC TTATGGTGGA CAGTGAGGAA 
GAGTACTTCC CTGAGGAGAT TGCTAAGCTG AGGAGGGACG TGGACAATGG CCTTTCCCTT 
GTCGTCTTCA GTGACTGGTA CAACACTTCT GTTATGAGAA AAGTGAAGTT TTACGATGAA 
AACACAAGGC AGTGGTGGAT GCCAGATACT GGAGGAGCCA ACGTCCCAGC TCTAAACGAG 
CTGCTGTCTG TGTGGAACAT GGGGTTCAGT GACGGCCTGT ATGAAGGGGA GTTTGCCCTG 
GCAAACCACG ACATGTACTA TGCATCGGGG TGCAGCATTG CCAGGTTTCC AGAAGATGGT 
GTGGTGATCA CACAGACTTT CAAGGACCAA GGATTGGAAG TCTTAAAACA AGAGACAGCA 
GTTGTCGACA ATGTCCCCAT TCTGGGGCTT TATCAGATTC CAGCTGAAGG TGGAGGCCGG 
ATTGTGCTGT ATGGAGACTC CAACTGCTTG GATGACAGTC ACAGACAGAA GGACTGCTTT 
TGGCTTCTGG ATGCACTCCT TCAGTACACA TCCTATGGTG TGACCCCTCC CAGCCTCAGC 
CATTCAGGGA ACCGGCAGCG CCCACCCAGC GGGGCTGGCT TGGCCCCTCC TGAAAGGATG 
GAAGGAAACC ACCTTCATCG CTACTCCAAA GTTCTTGAGG CCCACTTGGG AGACCCGAAA 
CCTCGGCCCC TTCCAGCCTG TCCACACTTG TCGTGGGCCA AGCCACAGCC TTTGAATGAG 
ACGGCGCCCA GTAATCTTTG GAAACACCAG AAGCTGCTCT CCATTGACCT GGACAAAGTA 
GTGTTACCCA ACTTTCGCTC AAATCGCCCT CAAGTGAGAC CTTTGTCCCC TGGAGAAAGT 
GGTGCCTGGG ACATTCCTGG AGGGATCATG CCTGGCCGCT ACAACCAGGA AGTAGGCCAG 
ACCATCCCTG TTTTTGCCTT CCTTGGAGCC ATGGTGGCCC TGGCCTTCTT CGTGGTACAG 
ATCAGTAAGG CCAAGAGCCG GCCGAAGCGG AGGAGGCCCA GGGCAAAGCG TCCACAACTT 
GCACAGCAGG CCCACCCTGC AAGGACCCCG TCAGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A key enzyme in the biogenesis of lysosomes is a protease that regulates cholesterol metabolism.
Science (New York, N.Y.)333(6038)87-90(2011 Jul)
Marschner K,Kollmann K,Schweizer M,Braulke T,Pohl S

Evolutionary conservation and adaptation in the mechanism that regulates SREBP action: what a long, strange tRIP it's been.
Genes & development23(22)2578-91(2009 Nov)
Osborne TF,Espenshade PJ

Sphingosine 1-phosphate accelerates wound healing in diabetic mice.
Journal of dermatological science48(1)53-60(2007 Oct)
Kawanabe T,Kawakami T,Yatomi Y,Shimada S,Soma Y

Tisp40, a spermatid specific bZip transcription factor, functions by binding to the unfolded protein response element via the Rip pathway.
Genes to cells : devoted to molecular & cellular mechanisms10(6)575-94(2005 Jun)
Nagamori I,Yabuta N,Fujii T,Tanaka H,Yomogida K,Nishimune Y,Nojima H

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Mbtps1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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