Angpt1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Angpt1
Entrez Gene ID	89807
Full Name	angiopoietin 1
Synonyms	Agpt,Agpt1,Ang-1
General protein information	Preferred Names angiopoietin 1 Names angiopoietin-1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 7 Map Location: 7q31
Summary	modulates endothelial cell survival and involved in the activation of endothelial cell-specific Tie-2 receptors [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_006241609.3	XP_006241671.1	angiopoietin-1 isoform X1
XM_008765464.2	XP_008763686.1	angiopoietin-1 isoform X3
XM_008765463.2	XP_008763685.1	angiopoietin-1 isoform X2
NM_053546.1	NP_445998.1	angiopoietin-1 precursor

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	ANGPT1	XP_519904.2
Bos taurus (cattle)	ANGPT1	NP_001070265.1
Xenopus tropicalis (tropical clawed frog)	angpt1	XP_002938959.2
	ANGPT1	XP_001088871.1
Rattus norvegicus (Norway rat)	Angpt1	NP_445998.1
Gallus gallus (chicken)	ANGPT1	NP_001186376.1
Canis lupus familiaris (dog)	ANGPT1	NP_001005754.1
Homo sapiens (human)	ANGPT1	NP_001137.2
Mus musculus (house mouse)	Angpt1	NP_033770.2
Danio rerio (zebrafish)	angpt1	NP_571888.1

Gene Ontology
Bibliography

Related articles in PubMed

Overexpression of Angiopoietin-1 Potentiates Endothelial Progenitor Cells for the Treatment of Aneurysm.
Lu Z, Wang Q, Chen C, Gao Y, He J, Liu W, Li Z, Zhao Z
Annals of vascular surgery(2017 Nov)

Differential regulation of blood flow-induced neovascularization and mural cell recruitment by vascular endothelial growth factor and angiopoietin signalling.
Stone OA, Carter JG, Lin PC, Paleolog E, Machado MJ, Bates DO
The Journal of physiology595(5)1575-1591(2017 Mar)

Compound 49b Restores Retinal Thickness and Reduces Degenerate Capillaries in the Rat Retina following Ischemia/Reperfusion.
Liu L, Jiang Y, Steinle JJ
PloS one11(7)e0159532(2016)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

We demonstrate that a fine balance of VEGF and angiopoietin signaling is required for the formation of a functional vascular network.
Title: Differential regulation of blood flow-induced neovascularization and mural cell recruitment by vascular endothelial growth factor and angiopoietin signalling.

Hypoxia significantly increased protein levels of VEGF, KDR, Angiopoiein 1, and Tie2, which were reduced following Compound 49b treatment. These data strongly suggested that Compound 49b protected the retina against I/R-induced injury. This provides additional support for a role of beta-adrenergic receptor actions in the retina.
Title: Compound 49b Restores Retinal Thickness and Reduces Degenerate Capillaries in the Rat Retina following Ischemia/Reperfusion.

Increased expression of Ang-1, Ang-2, Tie-2, and VEGF in ulcerative colitis-derived colorectal cancer might lead to chronic colitis and pathologic angiogenesis in rats.
Title: Angiopoietin and vascular endothelial growth factor expression in colorectal disease models.

COMP-Ang1-stimulated proliferation, differentiation, and migration of progenitor cells may involve the Tie-2-mediated activation of p38 MAPK and PI3K/Akt pathways
Title: COMP-angiopoietin 1 increases proliferation, differentiation, and migration of stem-like cells through Tie-2-mediated activation of p38 MAPK and PI3K/Akt signal transduction pathways.

Its signal pathway regulate age related inflammatory response via activating NF-kappaB and AP-1 transcription factor.
Title: Chinese prescription Kangen-karyu and Salviae Miltiorrhizae Radix improve age-related oxidative stress and inflammatory response through the PI3K/Akt or MAPK pathways.

The following Angpt1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Angpt1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa09851	NM_053546.1 Latest version!	Rattus norvegicus angiopoietin 1 (Angpt1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00
ORa30350	XM_006241609.3 Latest version!	Rattus norvegicus angiopoietin 1 (Angpt1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
ORa43884	XM_008765463.2 Latest version!	Rattus norvegicus angiopoietin 1 (Angpt1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
ORa43885	XM_008765464.2 Latest version!	Rattus norvegicus angiopoietin 1 (Angpt1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Angpt1 ( NM_053546.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_445998.1 Rattus norvegicus angiopoietin 1 (Angpt1), mRNA.

Price & Availability↑

CloneID	ORa09851
Clone ID Related Accession (Same CDS sequence)	NM_053546.1
Accession Version	NM_053546.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1494bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Rattus norvegicus(Norway rat)
Product	angiopoietin-1 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AB080023.1. On Jan 12, 2005 this sequence version replaced XM_346797.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB080023.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01906348 [ECO:0000348] ##Evidence-Data-END##

ATGACAGTTT TCCTTTCCTT TGCATTCTTC GCTGCCATTC TGACTCACAT AGGGTGCAGC 
AACCAGCGCC GGAGTCCAGA AAACGGAGGG AGAAGATATA ACCGAATTCA ACATGGGCAA 
TGTGCCTACA CTTTCATTCT TCCAGAACAC GACGGGAACT GCCGTGAGAG TGCGACAGAG 
CAGTACAACA CCAACGCTCT GCAAAGGGAT GCTCCACACG TGGAGACGGA TTTCTCTTCC 
CAGAAACTTC AGCATCTGGA GCATGTGATG GAAAATTATA CTCAGTGGCT GCAAAAACTT 
GAGAATTACA TTGTGGAAAA TATGAAGTCG GAGATGGCCC AGATACAACA GAATGCGGTT 
CAAAACCACA CGGCCACCAT GCTGGAGATA GGAACCAGCC TCTTGTCTCA GACTGCAGAG 
CAGACCCGAA AGCTCACAGA TGTGGAGACC CAGGTACTAA ATCAAACATC CCGTCTTGAA 
ATCCAACTGC TGGAGAATTC ATTATCAACA TACGAGCTAG AGAAACAGCT TCTCCAACAG 
ACAAATGAAA TTCTGAAGAT TCAGGAAAAA AACAGTTTAT TAGAGCATAA AATCCTAGAA 
ATGGAGGGAA AACACAAGGA AGAGCTGGAC ACCTTGAAGG AGGAGAAAGA AAACCTTCAA 
GGCTTGGTTA CTCGTCAGAC ATTCATCATC CAAGAATTGG AGAAGCAACT TAGCAGAGCT 
ACCAGCAACA ACAGTGTTCT GCAGAAGCAA CAACTGGAGC TCATGGACAC AGTCCATAAC 
CTTGTCAGCC TTTGCACAAA AGAAGTTTTG CTAAAGGGAG GAAAAAGAGA AGAAGAGAAA 
CCATTTCGAG ACTGTGCAGA TGTATATCAA GCTGGTTTTA ATAAGAGTGG AATCTACACT 
ATTTATTTTA ATAATATGCC AGAACCCAAA AAGGTATTTT GCAATATGGA TGTGAATGAA 
GGAGGATGGA CAGTAATACA ACACCGTGAG GATGGAAGCC TAGATTTCCA GAGGGGCTGG 
AAGGAGTATA AAATGGGTTT TGGGAATCCC TCTGGTGAAT ATTGGCTTGG GAACGAGTTC 
ATTTTTGCAA TAACCAGTCA GAGGCAGTAC ATGCTGAGGA TCGAGCTGAT GGACTGGGAA 
GGGAACCGAG CCTACTCACA GTACGACAGA TTCCACATAG GAAACCAGAA GCAGAACTAC 
AGGTTATATT TAAAGGGTCA CACGGGGACA GCAGGCAAAC AGAGCAGCTT GATCTTACAT 
GGTGCTGATT TCAGCACAAA GGACGCTGAT AACGACAACT GTATGTGCAA ATGCGCCCTT 
ATGCTAACAG GAGGTTGGTG GTTTGATGCC TGTGGCCCTT CCAATCTAAA CGGAATGTTC 
TACACTGCAG GGCAAAACCA TGGAAAACTG AATGGGATAA AGTGGCACTA CTTCAAAGGA 
CCCAGTTACT CCTTACGTTC CACAACCATG ATGATCCGGC CCTTGGACTT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_445998.1
CDS	292..1785
Translation	MTVFLSFAFFAAILTHIGCSNQRRSPENGGRRYNRIQHGQCAYTFILPEHDGNCRESATEQYNTNALQRDAPHVETDFSSQKLQHLEHVMENYTQWLQKLENYIVENMKSEMAQIQQNAVQNHTATMLEIGTSLLSQTAEQTRKLTDVETQVLNQTSRLEIQLLENSLSTYELEKQLLQQTNEILKIQEKNSLLEHKILEMEGKHKEELDTLKEEKENLQGLVTRQTFIIQELEKQLSRATSNNSVLQKQQLELMDTVHNLVSLCTKEVLLKGGKREEEKPFRDCADVYQAGFNKSGIYTIYFNNMPEPKKVFCNMDVNEGGWTVIQHREDGSLDFQRGWKEYKMGFGNPSGEYWLGNEFIFAITSQRQYMLRIELMDWEGNRAYSQYDRFHIGNQKQNYRLYLKGHTGTAGKQSSLILHGADFSTKDADNDNCMCKCALMLTGGWWFDACGPSNLNGMFYTAGQNHGKLNGIKWHYFKGPSYSLRSTTMMIRPLDF

Target ORF information:

RefSeq Version	NM_053546.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus angiopoietin 1 (Angpt1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_053546.1

ORF Insert Sequence:

ATGACAGTTT TCCTTTCCTT TGCATTCTTC GCTGCCATTC TGACTCACAT AGGGTGCAGC 
AACCAGCGCC GGAGTCCAGA AAACGGAGGG AGAAGATATA ACCGAATTCA ACATGGGCAA 
TGTGCCTACA CTTTCATTCT TCCAGAACAC GACGGGAACT GCCGTGAGAG TGCGACAGAG 
CAGTACAACA CCAACGCTCT GCAAAGGGAT GCTCCACACG TGGAGACGGA TTTCTCTTCC 
CAGAAACTTC AGCATCTGGA GCATGTGATG GAAAATTATA CTCAGTGGCT GCAAAAACTT 
GAGAATTACA TTGTGGAAAA TATGAAGTCG GAGATGGCCC AGATACAACA GAATGCGGTT 
CAAAACCACA CGGCCACCAT GCTGGAGATA GGAACCAGCC TCTTGTCTCA GACTGCAGAG 
CAGACCCGAA AGCTCACAGA TGTGGAGACC CAGGTACTAA ATCAAACATC CCGTCTTGAA 
ATCCAACTGC TGGAGAATTC ATTATCAACA TACGAGCTAG AGAAACAGCT TCTCCAACAG 
ACAAATGAAA TTCTGAAGAT TCAGGAAAAA AACAGTTTAT TAGAGCATAA AATCCTAGAA 
ATGGAGGGAA AACACAAGGA AGAGCTGGAC ACCTTGAAGG AGGAGAAAGA AAACCTTCAA 
GGCTTGGTTA CTCGTCAGAC ATTCATCATC CAAGAATTGG AGAAGCAACT TAGCAGAGCT 
ACCAGCAACA ACAGTGTTCT GCAGAAGCAA CAACTGGAGC TCATGGACAC AGTCCATAAC 
CTTGTCAGCC TTTGCACAAA AGAAGTTTTG CTAAAGGGAG GAAAAAGAGA AGAAGAGAAA 
CCATTTCGAG ACTGTGCAGA TGTATATCAA GCTGGTTTTA ATAAGAGTGG AATCTACACT 
ATTTATTTTA ATAATATGCC AGAACCCAAA AAGGTATTTT GCAATATGGA TGTGAATGAA 
GGAGGATGGA CAGTAATACA ACACCGTGAG GATGGAAGCC TAGATTTCCA GAGGGGCTGG 
AAGGAGTATA AAATGGGTTT TGGGAATCCC TCTGGTGAAT ATTGGCTTGG GAACGAGTTC 
ATTTTTGCAA TAACCAGTCA GAGGCAGTAC ATGCTGAGGA TCGAGCTGAT GGACTGGGAA 
GGGAACCGAG CCTACTCACA GTACGACAGA TTCCACATAG GAAACCAGAA GCAGAACTAC 
AGGTTATATT TAAAGGGTCA CACGGGGACA GCAGGCAAAC AGAGCAGCTT GATCTTACAT 
GGTGCTGATT TCAGCACAAA GGACGCTGAT AACGACAACT GTATGTGCAA ATGCGCCCTT 
ATGCTAACAG GAGGTTGGTG GTTTGATGCC TGTGGCCCTT CCAATCTAAA CGGAATGTTC 
TACACTGCAG GGCAAAACCA TGGAAAACTG AATGGGATAA AGTGGCACTA CTTCAAAGGA 
CCCAGTTACT CCTTACGTTC CACAACCATG ATGATCCGGC CCTTGGACTT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Angpt1 ( XM_006241609.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006241671.1 Rattus norvegicus angiopoietin 1 (Angpt1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa30350
Clone ID Related Accession (Same CDS sequence)	XM_006241609.3
Accession Version	XM_006241609.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1497bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	angiopoietin-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005106.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006241609.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACAGTTT TCCTTTCCTT TGCATTCTTC GCTGCCATTC TGACTCACAT AGGGTGCAGC 
AACCAGCGCC GGAGTCCAGA AAACGGAGGG AGAAGATATA ACCGAATTCA ACATGGGCAA 
TGTGCCTACA CTTTCATTCT TCCAGAACAC GACGGGAACT GCCGTGAGAG TGCGACAGAG 
CAGTACAACA CCAACGCTCT GCAAAGGGAT GCTCCACACG TGGAGACGGA TTTCTCTTCC 
CAGAAACTTC AGCATCTGGA GCATGTGATG GAAAATTATA CTCAGTGGCT GCAAAAACTT 
GAGAATTACA TTGTGGAAAA TATGAAGTCG GAGATGGCCC AGATACAACA GAATGCGGTT 
CAAAACCACA CGGCCACCAT GCTGGAGATA GGAACCAGCC TCTTGTCTCA GACTGCAGAG 
CAGACCCGAA AGCTCACAGA TGTGGAGACC CAGGTACTAA ATCAAACATC CCGTCTTGAA 
ATCCAACTGC TGGAGAATTC ATTATCAACA TACAAGCTAG AGAAACAGCT TCTCCAACAG 
ACAAATGAAA TTCTGAAGAT TCAGGAAAAA AACAGTTTAT TAGAGCATAA AATCCTAGAA 
ATGGAGGGAA AACACAAGGA AGAGCTGGAC ACCTTGAAGG AGGAGAAAGA AAACCTTCAA 
GGCTTGGTTA CTCGTCAGAC ATTCATCATC CAAGAATTGG AGAAGCAACT TAGCAGAGCT 
ACCAACAACA ACAGTGTTCT GCAGAAGCAA CAACTGGAGC TCATGGACAC AGTCCATAAC 
CTTGTCAGCC TTTGCACAAA AGAAGGTGTT TTGCTAAAGG GAGGAAAAAG AGAAGAAGAG 
AAACCATTTC GAGACTGTGC AGATGTATAT CAAGCTGGTT TTAATAAGAG TGGAATCTAC 
ACTATTTATT TTAATAATAT GCCAGAACCC AAAAAGGTAT TTTGCAATAT GGATGTGAAT 
GGAGGAGGAT GGACAGTAAT ACAACACCGT GAGGATGGAA GCCTAGATTT CCAGAGGGGC 
TGGAAGGAGT ATAAAATGGG TTTTGGGAAT CCCTCTGGTG AATATTGGCT TGGGAACGAG 
TTCATTTTTG CAATAACCAG TCAGAGGCAG TACATGCTGA GGATCGAGCT GATGGACTGG 
GAAGGGAACC GAGCCTACTC ACAGTACGAC AGATTCCACA TAGGAAACGA GAAGCAGAAC 
TACAGGTTAT ATTTAAAGGG TCACACGGGG ACAGCAGGCA AACAGAGCAG CTTGATCTTA 
CATGGTGCTG ATTTCAGCAC AAAGGACGCT GATAACGACA ACTGTATGTG CAAATGCGCC 
CTTATGCTAA CAGGAGGTTG GTGGTTTGAT GCCTGTGGCC CTTCCAATCT AAACGGAATG 
TTCTACACTG CGGGGCAAAA CCATGGAAAA CTGAATGGGA TAAAGTGGCA CTACTTCAAA 
GGACCCAGTT ACTCCTTACG TTCCACAACC ATGATGATCC GGCCCTTGGA CTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006241671.1
CDS	452..1948
Translation	MTVFLSFAFFAAILTHIGCSNQRRSPENGGRRYNRIQHGQCAYTFILPEHDGNCRESATEQYNTNALQRDAPHVETDFSSQKLQHLEHVMENYTQWLQKLENYIVENMKSEMAQIQQNAVQNHTATMLEIGTSLLSQTAEQTRKLTDVETQVLNQTSRLEIQLLENSLSTYKLEKQLLQQTNEILKIQEKNSLLEHKILEMEGKHKEELDTLKEEKENLQGLVTRQTFIIQELEKQLSRATNNNSVLQKQQLELMDTVHNLVSLCTKEGVLLKGGKREEEKPFRDCADVYQAGFNKSGIYTIYFNNMPEPKKVFCNMDVNGGGWTVIQHREDGSLDFQRGWKEYKMGFGNPSGEYWLGNEFIFAITSQRQYMLRIELMDWEGNRAYSQYDRFHIGNEKQNYRLYLKGHTGTAGKQSSLILHGADFSTKDADNDNCMCKCALMLTGGWWFDACGPSNLNGMFYTAGQNHGKLNGIKWHYFKGPSYSLRSTTMMIRPLDF

Target ORF information:

RefSeq Version	XM_006241609.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus angiopoietin 1 (Angpt1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006241609.3

ORF Insert Sequence:

ATGACAGTTT TCCTTTCCTT TGCATTCTTC GCTGCCATTC TGACTCACAT AGGGTGCAGC 
AACCAGCGCC GGAGTCCAGA AAACGGAGGG AGAAGATATA ACCGAATTCA ACATGGGCAA 
TGTGCCTACA CTTTCATTCT TCCAGAACAC GACGGGAACT GCCGTGAGAG TGCGACAGAG 
CAGTACAACA CCAACGCTCT GCAAAGGGAT GCTCCACACG TGGAGACGGA TTTCTCTTCC 
CAGAAACTTC AGCATCTGGA GCATGTGATG GAAAATTATA CTCAGTGGCT GCAAAAACTT 
GAGAATTACA TTGTGGAAAA TATGAAGTCG GAGATGGCCC AGATACAACA GAATGCGGTT 
CAAAACCACA CGGCCACCAT GCTGGAGATA GGAACCAGCC TCTTGTCTCA GACTGCAGAG 
CAGACCCGAA AGCTCACAGA TGTGGAGACC CAGGTACTAA ATCAAACATC CCGTCTTGAA 
ATCCAACTGC TGGAGAATTC ATTATCAACA TACAAGCTAG AGAAACAGCT TCTCCAACAG 
ACAAATGAAA TTCTGAAGAT TCAGGAAAAA AACAGTTTAT TAGAGCATAA AATCCTAGAA 
ATGGAGGGAA AACACAAGGA AGAGCTGGAC ACCTTGAAGG AGGAGAAAGA AAACCTTCAA 
GGCTTGGTTA CTCGTCAGAC ATTCATCATC CAAGAATTGG AGAAGCAACT TAGCAGAGCT 
ACCAACAACA ACAGTGTTCT GCAGAAGCAA CAACTGGAGC TCATGGACAC AGTCCATAAC 
CTTGTCAGCC TTTGCACAAA AGAAGGTGTT TTGCTAAAGG GAGGAAAAAG AGAAGAAGAG 
AAACCATTTC GAGACTGTGC AGATGTATAT CAAGCTGGTT TTAATAAGAG TGGAATCTAC 
ACTATTTATT TTAATAATAT GCCAGAACCC AAAAAGGTAT TTTGCAATAT GGATGTGAAT 
GGAGGAGGAT GGACAGTAAT ACAACACCGT GAGGATGGAA GCCTAGATTT CCAGAGGGGC 
TGGAAGGAGT ATAAAATGGG TTTTGGGAAT CCCTCTGGTG AATATTGGCT TGGGAACGAG 
TTCATTTTTG CAATAACCAG TCAGAGGCAG TACATGCTGA GGATCGAGCT GATGGACTGG 
GAAGGGAACC GAGCCTACTC ACAGTACGAC AGATTCCACA TAGGAAACGA GAAGCAGAAC 
TACAGGTTAT ATTTAAAGGG TCACACGGGG ACAGCAGGCA AACAGAGCAG CTTGATCTTA 
CATGGTGCTG ATTTCAGCAC AAAGGACGCT GATAACGACA ACTGTATGTG CAAATGCGCC 
CTTATGCTAA CAGGAGGTTG GTGGTTTGAT GCCTGTGGCC CTTCCAATCT AAACGGAATG 
TTCTACACTG CGGGGCAAAA CCATGGAAAA CTGAATGGGA TAAAGTGGCA CTACTTCAAA 
GGACCCAGTT ACTCCTTACG TTCCACAACC ATGATGATCC GGCCCTTGGA CTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Angpt1 ( XM_008765463.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008763685.1 Rattus norvegicus angiopoietin 1 (Angpt1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa43884
Clone ID Related Accession (Same CDS sequence)	XM_008765463.2
Accession Version	XM_008765463.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1227bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	angiopoietin-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005106.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008765463.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCATGA CGATTTGTGA TTACTATCTT GAGAATTACA TTGTGGAAAA TATGAAGTCG 
GAGATGGCCC AGATACAACA GAATGCGGTT CAAAACCACA CGGCCACCAT GCTGGAGATA 
GGAACCAGCC TCTTGTCTCA GACTGCAGAG CAGACCCGAA AGCTCACAGA TGTGGAGACC 
CAGGTACTAA ATCAAACATC CCGTCTTGAA ATCCAACTGC TGGAGAATTC ATTATCAACA 
TACAAGCTAG AGAAACAGCT TCTCCAACAG ACAAATGAAA TTCTGAAGAT TCAGGAAAAA 
AACAGTTTAT TAGAGCATAA AATCCTAGAA ATGGAGGGAA AACACAAGGA AGAGCTGGAC 
ACCTTGAAGG AGGAGAAAGA AAACCTTCAA GGCTTGGTTA CTCGTCAGAC ATTCATCATC 
CAAGAATTGG AGAAGCAACT TAGCAGAGCT ACCAACAACA ACAGTGTTCT GCAGAAGCAA 
CAACTGGAGC TCATGGACAC AGTCCATAAC CTTGTCAGCC TTTGCACAAA AGAAGGTGTT 
TTGCTAAAGG GAGGAAAAAG AGAAGAAGAG AAACCATTTC GAGACTGTGC AGATGTATAT 
CAAGCTGGTT TTAATAAGAG TGGAATCTAC ACTATTTATT TTAATAATAT GCCAGAACCC 
AAAAAGGTAT TTTGCAATAT GGATGTGAAT GGAGGAGGAT GGACAGTAAT ACAACACCGT 
GAGGATGGAA GCCTAGATTT CCAGAGGGGC TGGAAGGAGT ATAAAATGGG TTTTGGGAAT 
CCCTCTGGTG AATATTGGCT TGGGAACGAG TTCATTTTTG CAATAACCAG TCAGAGGCAG 
TACATGCTGA GGATCGAGCT GATGGACTGG GAAGGGAACC GAGCCTACTC ACAGTACGAC 
AGATTCCACA TAGGAAACGA GAAGCAGAAC TACAGGTTAT ATTTAAAGGG TCACACGGGG 
ACAGCAGGCA AACAGAGCAG CTTGATCTTA CATGGTGCTG ATTTCAGCAC AAAGGACGCT 
GATAACGACA ACTGTATGTG CAAATGCGCC CTTATGCTAA CAGGAGGTTG GTGGTTTGAT 
GCCTGTGGCC CTTCCAATCT AAACGGAATG TTCTACACTG CGGGGCAAAA CCATGGAAAA 
CTGAATGGGA TAAAGTGGCA CTACTTCAAA GGACCCAGTT ACTCCTTACG TTCCACAACC 
ATGATGATCC GGCCCTTGGA CTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008763685.1
CDS	89..1315
Translation	MFMTICDYYLENYIVENMKSEMAQIQQNAVQNHTATMLEIGTSLLSQTAEQTRKLTDVETQVLNQTSRLEIQLLENSLSTYKLEKQLLQQTNEILKIQEKNSLLEHKILEMEGKHKEELDTLKEEKENLQGLVTRQTFIIQELEKQLSRATNNNSVLQKQQLELMDTVHNLVSLCTKEGVLLKGGKREEEKPFRDCADVYQAGFNKSGIYTIYFNNMPEPKKVFCNMDVNGGGWTVIQHREDGSLDFQRGWKEYKMGFGNPSGEYWLGNEFIFAITSQRQYMLRIELMDWEGNRAYSQYDRFHIGNEKQNYRLYLKGHTGTAGKQSSLILHGADFSTKDADNDNCMCKCALMLTGGWWFDACGPSNLNGMFYTAGQNHGKLNGIKWHYFKGPSYSLRSTTMMIRPLDF

Target ORF information:

RefSeq Version	XM_008765463.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus angiopoietin 1 (Angpt1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008765463.2

ORF Insert Sequence:

ATGTTCATGA CGATTTGTGA TTACTATCTT GAGAATTACA TTGTGGAAAA TATGAAGTCG 
GAGATGGCCC AGATACAACA GAATGCGGTT CAAAACCACA CGGCCACCAT GCTGGAGATA 
GGAACCAGCC TCTTGTCTCA GACTGCAGAG CAGACCCGAA AGCTCACAGA TGTGGAGACC 
CAGGTACTAA ATCAAACATC CCGTCTTGAA ATCCAACTGC TGGAGAATTC ATTATCAACA 
TACAAGCTAG AGAAACAGCT TCTCCAACAG ACAAATGAAA TTCTGAAGAT TCAGGAAAAA 
AACAGTTTAT TAGAGCATAA AATCCTAGAA ATGGAGGGAA AACACAAGGA AGAGCTGGAC 
ACCTTGAAGG AGGAGAAAGA AAACCTTCAA GGCTTGGTTA CTCGTCAGAC ATTCATCATC 
CAAGAATTGG AGAAGCAACT TAGCAGAGCT ACCAACAACA ACAGTGTTCT GCAGAAGCAA 
CAACTGGAGC TCATGGACAC AGTCCATAAC CTTGTCAGCC TTTGCACAAA AGAAGGTGTT 
TTGCTAAAGG GAGGAAAAAG AGAAGAAGAG AAACCATTTC GAGACTGTGC AGATGTATAT 
CAAGCTGGTT TTAATAAGAG TGGAATCTAC ACTATTTATT TTAATAATAT GCCAGAACCC 
AAAAAGGTAT TTTGCAATAT GGATGTGAAT GGAGGAGGAT GGACAGTAAT ACAACACCGT 
GAGGATGGAA GCCTAGATTT CCAGAGGGGC TGGAAGGAGT ATAAAATGGG TTTTGGGAAT 
CCCTCTGGTG AATATTGGCT TGGGAACGAG TTCATTTTTG CAATAACCAG TCAGAGGCAG 
TACATGCTGA GGATCGAGCT GATGGACTGG GAAGGGAACC GAGCCTACTC ACAGTACGAC 
AGATTCCACA TAGGAAACGA GAAGCAGAAC TACAGGTTAT ATTTAAAGGG TCACACGGGG 
ACAGCAGGCA AACAGAGCAG CTTGATCTTA CATGGTGCTG ATTTCAGCAC AAAGGACGCT 
GATAACGACA ACTGTATGTG CAAATGCGCC CTTATGCTAA CAGGAGGTTG GTGGTTTGAT 
GCCTGTGGCC CTTCCAATCT AAACGGAATG TTCTACACTG CGGGGCAAAA CCATGGAAAA 
CTGAATGGGA TAAAGTGGCA CTACTTCAAA GGACCCAGTT ACTCCTTACG TTCCACAACC 
ATGATGATCC GGCCCTTGGA CTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Angpt1 ( XM_008765464.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008763686.1 Rattus norvegicus angiopoietin 1 (Angpt1), transcript variant X3, mRNA.

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CloneID	ORa43885
Clone ID Related Accession (Same CDS sequence)	XM_008765464.2
Accession Version	XM_008765464.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1218bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	angiopoietin-1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005106.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008765464.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAATT CTTCTGAGCT TGAGAATTAC ATTGTGGAAA ATATGAAGTC GGAGATGGCC 
CAGATACAAC AGAATGCGGT TCAAAACCAC ACGGCCACCA TGCTGGAGAT AGGAACCAGC 
CTCTTGTCTC AGACTGCAGA GCAGACCCGA AAGCTCACAG ATGTGGAGAC CCAGGTACTA 
AATCAAACAT CCCGTCTTGA AATCCAACTG CTGGAGAATT CATTATCAAC ATACAAGCTA 
GAGAAACAGC TTCTCCAACA GACAAATGAA ATTCTGAAGA TTCAGGAAAA AAACAGTTTA 
TTAGAGCATA AAATCCTAGA AATGGAGGGA AAACACAAGG AAGAGCTGGA CACCTTGAAG 
GAGGAGAAAG AAAACCTTCA AGGCTTGGTT ACTCGTCAGA CATTCATCAT CCAAGAATTG 
GAGAAGCAAC TTAGCAGAGC TACCAACAAC AACAGTGTTC TGCAGAAGCA ACAACTGGAG 
CTCATGGACA CAGTCCATAA CCTTGTCAGC CTTTGCACAA AAGAAGGTGT TTTGCTAAAG 
GGAGGAAAAA GAGAAGAAGA GAAACCATTT CGAGACTGTG CAGATGTATA TCAAGCTGGT 
TTTAATAAGA GTGGAATCTA CACTATTTAT TTTAATAATA TGCCAGAACC CAAAAAGGTA 
TTTTGCAATA TGGATGTGAA TGGAGGAGGA TGGACAGTAA TACAACACCG TGAGGATGGA 
AGCCTAGATT TCCAGAGGGG CTGGAAGGAG TATAAAATGG GTTTTGGGAA TCCCTCTGGT 
GAATATTGGC TTGGGAACGA GTTCATTTTT GCAATAACCA GTCAGAGGCA GTACATGCTG 
AGGATCGAGC TGATGGACTG GGAAGGGAAC CGAGCCTACT CACAGTACGA CAGATTCCAC 
ATAGGAAACG AGAAGCAGAA CTACAGGTTA TATTTAAAGG GTCACACGGG GACAGCAGGC 
AAACAGAGCA GCTTGATCTT ACATGGTGCT GATTTCAGCA CAAAGGACGC TGATAACGAC 
AACTGTATGT GCAAATGCGC CCTTATGCTA ACAGGAGGTT GGTGGTTTGA TGCCTGTGGC 
CCTTCCAATC TAAACGGAAT GTTCTACACT GCGGGGCAAA ACCATGGAAA ACTGAATGGG 
ATAAAGTGGC ACTACTTCAA AGGACCCAGT TACTCCTTAC GTTCCACAAC CATGATGATC 
CGGCCCTTGG ACTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008763686.1
CDS	4379..5596
Translation	MLNSSELENYIVENMKSEMAQIQQNAVQNHTATMLEIGTSLLSQTAEQTRKLTDVETQVLNQTSRLEIQLLENSLSTYKLEKQLLQQTNEILKIQEKNSLLEHKILEMEGKHKEELDTLKEEKENLQGLVTRQTFIIQELEKQLSRATNNNSVLQKQQLELMDTVHNLVSLCTKEGVLLKGGKREEEKPFRDCADVYQAGFNKSGIYTIYFNNMPEPKKVFCNMDVNGGGWTVIQHREDGSLDFQRGWKEYKMGFGNPSGEYWLGNEFIFAITSQRQYMLRIELMDWEGNRAYSQYDRFHIGNEKQNYRLYLKGHTGTAGKQSSLILHGADFSTKDADNDNCMCKCALMLTGGWWFDACGPSNLNGMFYTAGQNHGKLNGIKWHYFKGPSYSLRSTTMMIRPLDF

Target ORF information:

RefSeq Version	XM_008765464.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus angiopoietin 1 (Angpt1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008765464.2

ORF Insert Sequence:

ATGCTGAATT CTTCTGAGCT TGAGAATTAC ATTGTGGAAA ATATGAAGTC GGAGATGGCC 
CAGATACAAC AGAATGCGGT TCAAAACCAC ACGGCCACCA TGCTGGAGAT AGGAACCAGC 
CTCTTGTCTC AGACTGCAGA GCAGACCCGA AAGCTCACAG ATGTGGAGAC CCAGGTACTA 
AATCAAACAT CCCGTCTTGA AATCCAACTG CTGGAGAATT CATTATCAAC ATACAAGCTA 
GAGAAACAGC TTCTCCAACA GACAAATGAA ATTCTGAAGA TTCAGGAAAA AAACAGTTTA 
TTAGAGCATA AAATCCTAGA AATGGAGGGA AAACACAAGG AAGAGCTGGA CACCTTGAAG 
GAGGAGAAAG AAAACCTTCA AGGCTTGGTT ACTCGTCAGA CATTCATCAT CCAAGAATTG 
GAGAAGCAAC TTAGCAGAGC TACCAACAAC AACAGTGTTC TGCAGAAGCA ACAACTGGAG 
CTCATGGACA CAGTCCATAA CCTTGTCAGC CTTTGCACAA AAGAAGGTGT TTTGCTAAAG 
GGAGGAAAAA GAGAAGAAGA GAAACCATTT CGAGACTGTG CAGATGTATA TCAAGCTGGT 
TTTAATAAGA GTGGAATCTA CACTATTTAT TTTAATAATA TGCCAGAACC CAAAAAGGTA 
TTTTGCAATA TGGATGTGAA TGGAGGAGGA TGGACAGTAA TACAACACCG TGAGGATGGA 
AGCCTAGATT TCCAGAGGGG CTGGAAGGAG TATAAAATGG GTTTTGGGAA TCCCTCTGGT 
GAATATTGGC TTGGGAACGA GTTCATTTTT GCAATAACCA GTCAGAGGCA GTACATGCTG 
AGGATCGAGC TGATGGACTG GGAAGGGAAC CGAGCCTACT CACAGTACGA CAGATTCCAC 
ATAGGAAACG AGAAGCAGAA CTACAGGTTA TATTTAAAGG GTCACACGGG GACAGCAGGC 
AAACAGAGCA GCTTGATCTT ACATGGTGCT GATTTCAGCA CAAAGGACGC TGATAACGAC 
AACTGTATGT GCAAATGCGC CCTTATGCTA ACAGGAGGTT GGTGGTTTGA TGCCTGTGGC 
CCTTCCAATC TAAACGGAAT GTTCTACACT GCGGGGCAAA ACCATGGAAA ACTGAATGGG 
ATAAAGTGGC ACTACTTCAA AGGACCCAGT TACTCCTTAC GTTCCACAAC CATGATGATC 
CGGCCCTTGG ACTTCTGA