Sfrp4 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Sfrp4
Entrez Gene ID	89803
Full Name	secreted frizzled-related protein 4
General protein information	Preferred Names secreted frizzled-related protein 4 Names secreted frizzled-related protein 4 DDC-4 protein FRP frizzled related protein sFRP-4
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 17 Map Location: 17q11
Summary	Wnt binding protein involved in modulation of Wnt signaling [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_053544.1	NP_445996.1	secreted frizzled-related protein 4 precursor

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	SFRP4	XP_519048.3
Canis lupus familiaris (dog)	SFRP4	XP_540377.2
Gallus gallus (chicken)	SFRP4	XP_418831.4
	SFRP4	NP_001028134.1
Rattus norvegicus (Norway rat)	Sfrp4	NP_445996.1
Xenopus tropicalis (tropical clawed frog)	sfrp4	XP_002939467.2
Mus musculus (house mouse)	Sfrp4	NP_057896.1
Homo sapiens (human)	SFRP4	NP_003005.2
Bos taurus (cattle)	SFRP4	NP_001069232.1

Gene Ontology
Bibliography

Related articles in PubMed

Cortical-Bone Fragility--Insights from sFRP4 Deficiency in Pyle's Disease.
Kiper POS, Saito H, Gori F, Unger S, Hesse E, Yamana K, Kiviranta R, Solban N, Liu J, Brommage R, Boduroglu K, Bonaf? L, Campos-Xavier B, Dikoglu E, Eastell R, Gossiel F, Harshman K, Nishimura G, Girisha KM, Stevenson BJ, Takita H, Rivolta C, Superti-Furga A, Baron R
The New England journal of medicine374(26)2553-2562(2016 Jun)

MeCP2 modulates the canonical Wnt pathway activation by targeting SFRP4 in rheumatoid arthritis fibroblast-like synoviocytes in rats.
Miao CG, Huang C, Huang Y, Yang YY, He X, Zhang L, Lv XW, Jin Y, Li J
Cellular signalling25(3)598-608(2013 Mar)

Secreted and transmembrane wnt inhibitors and activators.
Cruciat CM, Niehrs C
Cold Spring Harbor perspectives in biology5(3)a015081(2013 Mar)

Hydroxytyrosol inhibits growth and cell proliferation and promotes high expression of sfrp4 in rat mammary tumours.
Granados-Principal S, Quiles JL, Ramirez-Tortosa C, Camacho-Corencia P, Sanchez-Rovira P, Vera-Ramirez L, Ramirez-Tortosa MC
Molecular nutrition & food research55 Suppl 1S117-26(2011 May)

Secreted frizzled related protein 4 reduces fibrosis scar size and ameliorates cardiac function after ischemic injury.
Matsushima K, Suyama T, Takenaka C, Nishishita N, Ikeda K, Ikada Y, Sawa Y, Jakt LM, Mori H, Kawamata S
Tissue engineering. Part A16(11)3329-41(2010 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Increased SFRP4 down-regulated the key gene beta-catenin, the downstream effectors gene ccnd1 and fibronectin expression in canonical Wnt pathway at the same time.
Title: MeCP2 modulates the canonical Wnt pathway activation by targeting SFRP4 in rheumatoid arthritis fibroblast-like synoviocytes in rats.

Hydroxytyrosol alters several genes associated with cell proliferation, apoptosis and the Wnt signalling pathway, promoting a high expression of Sfrp4 in rat mammary tumors.
Title: Hydroxytyrosol inhibits growth and cell proliferation and promotes high expression of sfrp4 in rat mammary tumours.

observations support the hypothesis that increased placental expression of SFRP4 inhibits the WNT pathway and thereby influences placental growth via effects on cell fate signaling
Title: Placental expression of secreted frizzled related protein-4 in the rat and the impact of glucocorticoid-induced fetal and placental growth restriction.

Regulation of sFRP-4 by LH and PRL may be important for modulating Fz-1, known to be expressed in periovulatory follicles, and Wnt-4/Fz-4, which are expressed in corpora lutea.
Title: Expression and localization of secreted frizzled-related protein-4 in the rodent ovary: evidence for selective up-regulation in luteinized granulosa cells.

This study demonstrates an association between Secreted Frizzled Related Protein 4 and apoptosis during rat ovulation.
Title: The role of sFRP4, a secreted frizzled-related protein, in ovulation.

The following Sfrp4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Sfrp4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa09845	NM_053544.1 Latest version!	Rattus norvegicus secreted frizzled-related protein 4 (Sfrp4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Sfrp4 ( NM_053544.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_445996.1 Rattus norvegicus secreted frizzled-related protein 4 (Sfrp4), mRNA.

Price & Availability↑

CloneID	ORa09845
Clone ID Related Accession (Same CDS sequence)	NM_053544.1
Accession Version	NM_053544.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1047bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-15
Organism	Rattus norvegicus(Norway rat)
Product	secreted frizzled-related protein 4 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF012891.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF012891.1, AF140346.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01906350, SAMN01906356 [ECO:0000348] ##Evidence-Data-END##

ATGCTCCTCT CCATCCTGGT AGCGTTATGC CTGTGCGTGC GCCTGGCTCT GGGAGTGCGC 
GGAGCGCCCT GCGAGGCTGT GCGCATCCCC ATGTGTAGGC ACATGCCCTG GAACATCACC 
CGGATGCCCA ACCACCTGCA CCACAGCACT CAGGAGAACG CCATCCTGGC CATCGAGCAG 
TACGAGGAGC TGGTAGACGT GAACTGCAGC TCTGTACTGC GCTTCTTTCT CTGTGCCATG 
TATGCACCCA TCTGTACCCT GGAGTTCCTG CACGATCCCA TCAAACCCTG CAAATCTGTG 
TGCCAGCGCG CACGCGACGA CTGCGAGCCC CTCATGAAGA TGTATAACCA CAGCTGGCCA 
GAGAGCCTGG CTTGCGATGA GCTGCCTGTC TATGACCGTG GAGTGTGCAT CTCTCCGGAG 
GCGATAGTCA CTGACCTTCC GGAAGATGTG AAGTGGATAG ACATCACACC AGATATGATG 
GTTCAAGAAA GGTCCTTTGA TGCTGACTGT AAACATCTGA GCCCTGATCG GTGCAAGTGC 
AAAAAGGTGA AGCCAACTTT GGCAACGTAC CTGAGCAAAA ACTACAGCTA TGTTATTCAT 
GCCAAAATAA AAGCGGTCCA GAGGAGTGGT TGCAATGAGG TCACAACTGT GGTCGATGTA 
AAAGAGATCT TCAAGTCTTC ATCACCTATC CCTCGAACGC AAGTCCCCCT CATCACCAAT 
TCCTCCTGCC AGTGTCCACA CATCCTGCCC CNTCAAGATG TCCTAATCAT GTGTTATGAG 
CGGCGTTCAA GGATGATGCT TCTTGAAAAT TGTTTAGTTG AGAAATGGAG AGATCAACTA 
AGCAGAAGGT CCACACAGTG GGAAGAGAGG CTTCAGGAAC AGCAGAGAAC AACTCAGGAC 
AAGAAGCAAA TAGCCAGCCG CACCAGTCGC AGTAACCCCC CAAAGCCAAA GGGAAGGTCA 
CCTGCTTCCA AACCTGCCAG TCCTAAGAAG AACATCAAAG CTAGAAGTGC ACCCAAAAAG 
TCAAACCCAA AGAAAAGTAC AAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_445996.1
CDS	95..1141
Translation	MLLSILVALCLCVRLALGVRGAPCEAVRIPMCRHMPWNITRMPNHLHHSTQENAILAIEQYEELVDVNCSSVLRFFLCAMYAPICTLEFLHDPIKPCKSVCQRARDDCEPLMKMYNHSWPESLACDELPVYDRGVCISPEAIVTDLPEDVKWIDITPDMMVQERSFDADCKHLSPDRCKCKKVKPTLATYLSKNYSYVIHAKIKAVQRSGCNEVTTVVDVKEIFKSSSPIPRTQVPLITNSSCQCPHILPnullQDVLIMCYERRSRMMLLENCLVEKWRDQLSRRSTQWEERLQEQQRTTQDKKQIASRTSRSNPPKPKGRSPASKPASPKKNIKARSAPKKSNPKKSTS

Target ORF information:

RefSeq Version	NM_053544.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus secreted frizzled-related protein 4 (Sfrp4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_053544.1

ORF Insert Sequence:

ATGCTCCTCT CCATCCTGGT AGCGTTATGC CTGTGCGTGC GCCTGGCTCT GGGAGTGCGC 
GGAGCGCCCT GCGAGGCTGT GCGCATCCCC ATGTGTAGGC ACATGCCCTG GAACATCACC 
CGGATGCCCA ACCACCTGCA CCACAGCACT CAGGAGAACG CCATCCTGGC CATCGAGCAG 
TACGAGGAGC TGGTAGACGT GAACTGCAGC TCTGTACTGC GCTTCTTTCT CTGTGCCATG 
TATGCACCCA TCTGTACCCT GGAGTTCCTG CACGATCCCA TCAAACCCTG CAAATCTGTG 
TGCCAGCGCG CACGCGACGA CTGCGAGCCC CTCATGAAGA TGTATAACCA CAGCTGGCCA 
GAGAGCCTGG CTTGCGATGA GCTGCCTGTC TATGACCGTG GAGTGTGCAT CTCTCCGGAG 
GCGATAGTCA CTGACCTTCC GGAAGATGTG AAGTGGATAG ACATCACACC AGATATGATG 
GTTCAAGAAA GGTCCTTTGA TGCTGACTGT AAACATCTGA GCCCTGATCG GTGCAAGTGC 
AAAAAGGTGA AGCCAACTTT GGCAACGTAC CTGAGCAAAA ACTACAGCTA TGTTATTCAT 
GCCAAAATAA AAGCGGTCCA GAGGAGTGGT TGCAATGAGG TCACAACTGT GGTCGATGTA 
AAAGAGATCT TCAAGTCTTC ATCACCTATC CCTCGAACGC AAGTCCCCCT CATCACCAAT 
TCCTCCTGCC AGTGTCCACA CATCCTGCCC CNTCAAGATG TCCTAATCAT GTGTTATGAG 
CGGCGTTCAA GGATGATGCT TCTTGAAAAT TGTTTAGTTG AGAAATGGAG AGATCAACTA 
AGCAGAAGGT CCACACAGTG GGAAGAGAGG CTTCAGGAAC AGCAGAGAAC AACTCAGGAC 
AAGAAGCAAA TAGCCAGCCG CACCAGTCGC AGTAACCCCC CAAAGCCAAA GGGAAGGTCA 
CCTGCTTCCA AACCTGCCAG TCCTAAGAAG AACATCAAAG CTAGAAGTGC ACCCAAAAAG 
TCAAACCCAA AGAAAAGTAC AAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Cortical-Bone Fragility--Insights from sFRP4 Deficiency in Pyle's Disease.
The New England journal of medicine374(26)2553-2562(2016 Jun)
Kiper POS,Saito H,Gori F,Unger S,Hesse E,Yamana K,Kiviranta R,Solban N,Liu J,Brommage R,Boduroglu K,Bonaf? L,Campos-Xavier B,Dikoglu E,Eastell R,Gossiel F,Harshman K,Nishimura G,Girisha KM,Stevenson BJ,Takita H,Rivolta C,Superti-Furga A,Baron R

MeCP2 modulates the canonical Wnt pathway activation by targeting SFRP4 in rheumatoid arthritis fibroblast-like synoviocytes in rats.
Cellular signalling25(3)598-608(2013 Mar)
Miao CG,Huang C,Huang Y,Yang YY,He X,Zhang L,Lv XW,Jin Y,Li J

Secreted and transmembrane wnt inhibitors and activators.
Cold Spring Harbor perspectives in biology5(3)a015081(2013 Mar)
Cruciat CM,Niehrs C

Hydroxytyrosol inhibits growth and cell proliferation and promotes high expression of sfrp4 in rat mammary tumours.
Molecular nutrition & food research55 Suppl 1S117-26(2011 May)
Granados-Principal S,Quiles JL,Ramirez-Tortosa C,Camacho-Corencia P,Sanchez-Rovira P,Vera-Ramirez L,Ramirez-Tortosa MC

Secreted frizzled related protein 4 reduces fibrosis scar size and ameliorates cardiac function after ischemic injury.
Tissue engineering. Part A16(11)3329-41(2010 Nov)
Matsushima K,Suyama T,Takenaka C,Nishishita N,Ikeda K,Ikada Y,Sawa Y,Jakt LM,Mori H,Kawamata S

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Sfrp4 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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