Abcf1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Abcf1
Entrez Gene ID	85493
Full Name	ATP binding cassette subfamily F member 1
Synonyms	Abc50
General protein information	Preferred Names ATP binding cassette subfamily F member 1 Names ATP-binding cassette sub-family F member 1 ATP-binding cassette 50 ATP-binding cassette, sub-family F (GCN20), member 1 ATP-binding cassette, subfamily F (GCN20), member 1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 20 Map Location: 20p12
Summary	ATP-binding cassette (ABC) protein family member that associates with eIF2 and ribosomes to facilitate mRNA translation [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_017601783.1	XP_017457272.1	ATP-binding cassette sub-family F member 1 isoform X1
NM_001109883.2	NP_001103353.1	ATP-binding cassette sub-family F member 1

Pathways from BioSystems

Homologies

Oryza sativa (rice)	Os03g0441500	NP_001050461.1
Mus musculus (house mouse)	Abcf1	NP_038882.1
Arabidopsis thaliana (thale cress)	GCN4	NP_567001.1
Rattus norvegicus (Norway rat)	Abcf1	NP_001103353.1
Danio rerio (zebrafish)	abcf1	NP_998351.1
Drosophila melanogaster (fruit fly)	CG1703	NP_572736.1
	ABCF1	NP_001098632.1
Canis lupus familiaris (dog)	ABCF1	XP_532056.4
Caenorhabditis elegans (roundworm)	abcf-1	NP_506192.1
Homo sapiens (human)	ABCF1	NP_001081.1
Pan troglodytes (chimpanzee)	ABCF1	NP_001035838.1
Bos taurus (cattle)	ABCF1	NP_001193838.1
Xenopus tropicalis (tropical clawed frog)	abcf1	XP_002939514.2

Gene Ontology
Bibliography

Related articles in PubMed

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Castello A, Fischer B, Eichelbaum K, Horos R, Beckmann BM, Strein C, Davey NE, Humphreys DT, Preiss T, Steinmetz LM, Krijgsveld J, Hentze MW
Cell149(6)1393-406(2012 Jun)

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Baltz AG, Munschauer M, Schwanh?usser B, Vasile A, Murakawa Y, Schueler M, Youngs N, Penfold-Brown D, Drew K, Milek M, Wyler E, Bonneau R, Selbach M, Dieterich C, Landthaler M
Molecular cell46(5)674-90(2012 Jun)

Defining the membrane proteome of NK cells.
Ghosh D, Lippert D, Krokhin O, Cortens JP, Wilkins JA
Journal of mass spectrometry : JMS45(1)1-25(2010 Jan)

ABC50 promotes translation initiation in mammalian cells.
Paytubi S, Wang X, Lam YW, Izquierdo L, Hunter MJ, Jan E, Hundal HS, Proud CG
The Journal of biological chemistry284(36)24061-73(2009 Sep)

The N-terminal region of ABC50 interacts with eukaryotic initiation factor eIF2 and is a target for regulatory phosphorylation by CK2.
Paytubi S, Morrice NA, Boudeau J, Proud CG
The Biochemical journal409(1)223-31(2008 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Abcf1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Abcf1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa00666	NM_001109883.2 Latest version!	Rattus norvegicus ATP binding cassette subfamily F member 1 (Abcf1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$475.30 $679.00
ORa60194	XM_017601783.1 Latest version!	Rattus norvegicus ATP binding cassette subfamily F member 1 (Abcf1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	10-12	$391.30 $559.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Abcf1 ( NM_001109883.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001103353.1 Rattus norvegicus ATP binding cassette subfamily F member 1 (Abcf1), mRNA.

Price & Availability↑

CloneID	ORa00666
Clone ID Related Accession (Same CDS sequence)	NM_001109883.2
Accession Version	NM_001109883.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2520bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Rattus norvegicus(Norway rat)
Product	ATP-binding cassette sub-family F member 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX883048.1, EV773343.1, CK356298.1, CB801303.1, CV114142.1, EV771702.1 and BP495802.1. On Oct 16, 2009 this sequence version replaced NM_001109883.1. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGCCGAAGG GTCCCAAGCA GCAGCCGCCC GAGCCCGAGT GGATCGGGGA CGGCGAAGGC 
ACGAGCCCCG CGGACAAAGT AGTGAAGAAA GGCAAAAAAG ACAAGAAGAC CAAAAAGACG 
TTCTTTGAGG AACTGGCAGT GGAAGACAAG CAAGCCGGGG AAGAGGAGAA ACTGCAGAAG 
GAGAAGGAGC AGCAGCAGCA GCAGCAGCAA CAGAAGAAAA AGCGAGACAC CAGGAAAGGT 
CGTCGGAAGA AGGATGTGGA TGATGATGAT GATGGTGATG AGAGAGTGCT CATGGAGCGC 
CTTAAGCAGC TGTCTGTGCC AGCCAGTGAT GAGGAAGATG AGGTACCTGT CCCCGTGCCC 
CGAGGACGGA AGAAGGCAAA GGGCGGAAAT GTTTTTGAAG CCCTGATTCA GGATGAAAGT 
GAGGAGGAAA AAGAAGAGGA GGAAGAAAAG CCTGTTCTCA AGCCTGCCAA GCCAGAGAAG 
AATCGCATCA ATAAGGCCGT GGCTGAGGAG CCTCCTGGGC TCCGAAATAA AAAGGGAAAG 
GAGGAGAAAT CGAAAGGGAA AGCCAAGAAT AAACCGTCTG CTACAGACAG TGAAGGGGAA 
GATGATGAGG ACATGACTAA AGAAAAGGAG CCTCCCAGGC CAGGGAAGGA CAAAGACAAA 
AAGGGAGCTG AGCAGGGTTC AGAGGAAGAG AAAGAAGAGA AGGAGGGGGA GGTGAAGGCG 
AATGATCCCT ATGCCCACCT TAGCAAAAAG GAAAAGAAAA AGCTGAAGAA ACAGATGGAT 
TATGAACGCC AGGTGGAATC ATTAAAGGCA GCTAATGCTG CAGAAAATGA CTTCTCTGTG 
TCCCAGGCAG AGGTGTCTTC CCGCCAGGCA ATGTTAGAAA ATGCATCTGA CATTAAGTTG 
GAAAAGTTCA GCATCTCGGC CCACGGCAAG GAGCTGTTTG TCAATGCTGA CCTGTACATC 
GTGGCTGGCC GCCGCTATGG GCTGGTGGGG CCCAACGGCA AGGGCAAGAC CACACTTCTG 
AAGCACATTG CCAACCGTGC CCTGAGTATC CCCCCTAACA TTGATGTGCT GCTGTGCGAG 
CAGGAGGTGG TGGCTGATGA AACACCAGCC GTGCAAGCTG TTCTTCGTGC AGACACAAAG 
CGACTGAGGT TGCTAGAGGA GGAGAAACGG CTTCAGGGAC AGCTGGAGCA GGGGGATGAT 
ACTGCCGCTG AGAAACTAGA AAAGGTATAT GAGGAACTGC GAGCTACTGG GGCAGCAGCT 
GCAGAGGCCA AGGCACGGCG GATCCTGGCT GGCTTGGGCT TTGACCCTGA GATGCAGAAT 
CGGCCCACAC AGAAGTTCTC TGGGGGTTGG CGAATGCGTG TCTCCCTGGC CAGGGCACTG 
TTCATGGAAC CCACACTGCT GATGCTGGAC GAGCCTACCA ATCACCTGGA CCTCAATGCC 
GTCATCTGGC TCAATAACTA CCTTCAGGGC TGGAGGAAGA CACTGCTGAT CGTCTCCCAC 
GACCAGGGCT TTCTGGATGA TGTCTGCACC GATATCATCC ACCTGGACAC TCAGCGGCTC 
CATTACTACA GGGGCAATTA CATGACCTTC AAGAAGATGT ACCAGCAGAA GCAGAAGGAG 
CTGCTGAAGC AGTACGAGAA ACAGGAGAAG AAGCTGAAGG AGCTGAAGGC TGGGGGCAAG 
TCCACCAAGC AAGCGGAAAA GCAAACAAAG GAAGTCCTGA CTCGAAAACA GCAGAAATGC 
CGAAGGAAAA ACCAGGATGA GGAGTCTCAG GATCCCCCTG AGCTTCTGAA GCGCCCCAGG 
GAGTACACTG TGCGATTCAC CTTCCCAGAC CCCCCACCTC TCAGCCCACC TGTCCTGGGG 
CTGCATGGTG TGACGTTTGG CTACGAGGGG CAGAAGCCAC TCTTTAAGAA CCTGGATTTC 
GGCATCGACA TGGACTCCCG AATTTGCATT GTGGGTCCCA ATGGTGTGGG GAAGAGCACA 
CTACTCCTGT TGCTGACTGG CAAGCTGACA CCGACCAACG GGGAAATGAG GAAGAACCAT 
CGGCTGAAAA TCGGCTTCTT TAACCAGCAG TATGCAGAGC AGCTGCACAT GGAGGAGACG 
CCCACCGAGT ACCTGCAGCG GGGCTTCAAC CTGCCCTATC AGGATGCCCG GAAGTGCTTG 
GGCCGCTTCG GCCTGGAGAG CCACGCCCAC ACCATCCAGA TCTGCAAACT CTCGGGCGGG 
CAGAAAGCCC GAGTTGTGTT TGCGGAGCTG GCCTGTCGGG AGCCTGATGT CCTCATCTTG 
GATGAACCAA CCAATAACTT GGACATAGAG TCCATCGATG CCCTGGGAGA GGCCATCAAC 
GAGTACAAGG GAGCTGTGAT CGTTGTCAGC CATGATGCAC GCCTCATCAC AGAAACCAAC 
TGCCAGTTGT GGGTCGTGGA GGAGCAGAGT GTCAGTCAAA TTGATGGTGA CTTTGATGAC 
TACAAGCGAG AGGTGTTGGA GGCCCTGGGT GAAGTCATGG TCAACCGACC TCGGGATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001103353.1
CDS	34..2553
Translation	MPKGPKQQPPEPEWIGDGEGTSPADKVVKKGKKDKKTKKTFFEELAVEDKQAGEEEKLQKEKEQQQQQQQQKKKRDTRKGRRKKDVDDDDDGDERVLMERLKQLSVPASDEEDEVPVPVPRGRKKAKGGNVFEALIQDESEEEKEEEEEKPVLKPAKPEKNRINKAVAEEPPGLRNKKGKEEKSKGKAKNKPSATDSEGEDDEDMTKEKEPPRPGKDKDKKGAEQGSEEEKEEKEGEVKANDPYAHLSKKEKKKLKKQMDYERQVESLKAANAAENDFSVSQAEVSSRQAMLENASDIKLEKFSISAHGKELFVNADLYIVAGRRYGLVGPNGKGKTTLLKHIANRALSIPPNIDVLLCEQEVVADETPAVQAVLRADTKRLRLLEEEKRLQGQLEQGDDTAAEKLEKVYEELRATGAAAAEAKARRILAGLGFDPEMQNRPTQKFSGGWRMRVSLARALFMEPTLLMLDEPTNHLDLNAVIWLNNYLQGWRKTLLIVSHDQGFLDDVCTDIIHLDTQRLHYYRGNYMTFKKMYQQKQKELLKQYEKQEKKLKELKAGGKSTKQAEKQTKEVLTRKQQKCRRKNQDEESQDPPELLKRPREYTVRFTFPDPPPLSPPVLGLHGVTFGYEGQKPLFKNLDFGIDMDSRICIVGPNGVGKSTLLLLLTGKLTPTNGEMRKNHRLKIGFFNQQYAEQLHMEETPTEYLQRGFNLPYQDARKCLGRFGLESHAHTIQICKLSGGQKARVVFAELACREPDVLILDEPTNNLDIESIDALGEAINEYKGAVIVVSHDARLITETNCQLWVVEEQSVSQIDGDFDDYKREVLEALGEVMVNRPRD

Target ORF information:

RefSeq Version	NM_001109883.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ATP binding cassette subfamily F member 1 (Abcf1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001109883.2

ORF Insert Sequence:

ATGCCGAAGG GTCCCAAGCA GCAGCCGCCC GAGCCCGAGT GGATCGGGGA CGGCGAAGGC 
ACGAGCCCCG CGGACAAAGT AGTGAAGAAA GGCAAAAAAG ACAAGAAGAC CAAAAAGACG 
TTCTTTGAGG AACTGGCAGT GGAAGACAAG CAAGCCGGGG AAGAGGAGAA ACTGCAGAAG 
GAGAAGGAGC AGCAGCAGCA GCAGCAGCAA CAGAAGAAAA AGCGAGACAC CAGGAAAGGT 
CGTCGGAAGA AGGATGTGGA TGATGATGAT GATGGTGATG AGAGAGTGCT CATGGAGCGC 
CTTAAGCAGC TGTCTGTGCC AGCCAGTGAT GAGGAAGATG AGGTACCTGT CCCCGTGCCC 
CGAGGACGGA AGAAGGCAAA GGGCGGAAAT GTTTTTGAAG CCCTGATTCA GGATGAAAGT 
GAGGAGGAAA AAGAAGAGGA GGAAGAAAAG CCTGTTCTCA AGCCTGCCAA GCCAGAGAAG 
AATCGCATCA ATAAGGCCGT GGCTGAGGAG CCTCCTGGGC TCCGAAATAA AAAGGGAAAG 
GAGGAGAAAT CGAAAGGGAA AGCCAAGAAT AAACCGTCTG CTACAGACAG TGAAGGGGAA 
GATGATGAGG ACATGACTAA AGAAAAGGAG CCTCCCAGGC CAGGGAAGGA CAAAGACAAA 
AAGGGAGCTG AGCAGGGTTC AGAGGAAGAG AAAGAAGAGA AGGAGGGGGA GGTGAAGGCG 
AATGATCCCT ATGCCCACCT TAGCAAAAAG GAAAAGAAAA AGCTGAAGAA ACAGATGGAT 
TATGAACGCC AGGTGGAATC ATTAAAGGCA GCTAATGCTG CAGAAAATGA CTTCTCTGTG 
TCCCAGGCAG AGGTGTCTTC CCGCCAGGCA ATGTTAGAAA ATGCATCTGA CATTAAGTTG 
GAAAAGTTCA GCATCTCGGC CCACGGCAAG GAGCTGTTTG TCAATGCTGA CCTGTACATC 
GTGGCTGGCC GCCGCTATGG GCTGGTGGGG CCCAACGGCA AGGGCAAGAC CACACTTCTG 
AAGCACATTG CCAACCGTGC CCTGAGTATC CCCCCTAACA TTGATGTGCT GCTGTGCGAG 
CAGGAGGTGG TGGCTGATGA AACACCAGCC GTGCAAGCTG TTCTTCGTGC AGACACAAAG 
CGACTGAGGT TGCTAGAGGA GGAGAAACGG CTTCAGGGAC AGCTGGAGCA GGGGGATGAT 
ACTGCCGCTG AGAAACTAGA AAAGGTATAT GAGGAACTGC GAGCTACTGG GGCAGCAGCT 
GCAGAGGCCA AGGCACGGCG GATCCTGGCT GGCTTGGGCT TTGACCCTGA GATGCAGAAT 
CGGCCCACAC AGAAGTTCTC TGGGGGTTGG CGAATGCGTG TCTCCCTGGC CAGGGCACTG 
TTCATGGAAC CCACACTGCT GATGCTGGAC GAGCCTACCA ATCACCTGGA CCTCAATGCC 
GTCATCTGGC TCAATAACTA CCTTCAGGGC TGGAGGAAGA CACTGCTGAT CGTCTCCCAC 
GACCAGGGCT TTCTGGATGA TGTCTGCACC GATATCATCC ACCTGGACAC TCAGCGGCTC 
CATTACTACA GGGGCAATTA CATGACCTTC AAGAAGATGT ACCAGCAGAA GCAGAAGGAG 
CTGCTGAAGC AGTACGAGAA ACAGGAGAAG AAGCTGAAGG AGCTGAAGGC TGGGGGCAAG 
TCCACCAAGC AAGCGGAAAA GCAAACAAAG GAAGTCCTGA CTCGAAAACA GCAGAAATGC 
CGAAGGAAAA ACCAGGATGA GGAGTCTCAG GATCCCCCTG AGCTTCTGAA GCGCCCCAGG 
GAGTACACTG TGCGATTCAC CTTCCCAGAC CCCCCACCTC TCAGCCCACC TGTCCTGGGG 
CTGCATGGTG TGACGTTTGG CTACGAGGGG CAGAAGCCAC TCTTTAAGAA CCTGGATTTC 
GGCATCGACA TGGACTCCCG AATTTGCATT GTGGGTCCCA ATGGTGTGGG GAAGAGCACA 
CTACTCCTGT TGCTGACTGG CAAGCTGACA CCGACCAACG GGGAAATGAG GAAGAACCAT 
CGGCTGAAAA TCGGCTTCTT TAACCAGCAG TATGCAGAGC AGCTGCACAT GGAGGAGACG 
CCCACCGAGT ACCTGCAGCG GGGCTTCAAC CTGCCCTATC AGGATGCCCG GAAGTGCTTG 
GGCCGCTTCG GCCTGGAGAG CCACGCCCAC ACCATCCAGA TCTGCAAACT CTCGGGCGGG 
CAGAAAGCCC GAGTTGTGTT TGCGGAGCTG GCCTGTCGGG AGCCTGATGT CCTCATCTTG 
GATGAACCAA CCAATAACTT GGACATAGAG TCCATCGATG CCCTGGGAGA GGCCATCAAC 
GAGTACAAGG GAGCTGTGAT CGTTGTCAGC CATGATGCAC GCCTCATCAC AGAAACCAAC 
TGCCAGTTGT GGGTCGTGGA GGAGCAGAGT GTCAGTCAAA TTGATGGTGA CTTTGATGAC 
TACAAGCGAG AGGTGTTGGA GGCCCTGGGT GAAGTCATGG TCAACCGACC TCGGGATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Abcf1 ( XM_017601783.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017457272.1 Rattus norvegicus ATP binding cassette subfamily F member 1 (Abcf1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa60194
Clone ID Related Accession (Same CDS sequence)	XM_017601783.1
Accession Version	XM_017601783.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2229bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	ATP-binding cassette sub-family F member 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005119.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCGCC TTAAGCAGCT GTCTGTGCCA GCCAGTGATG AGGAAGATGA GGTACCTGTC 
CCCGTGCCCC GAGGACGGAA GAAGGCAAAG GGCGGAAATG TTTTTGAAGC CCTGATTCAG 
GATGAAAGTG AGGAGGAAAA AGAAGAGGAG GAAGAAAAGC CTGTTCTCAA GCCTGCCAAG 
CCAGAGAAGA ATCGCATCAA TAAGGCCGTG GCTGAGGAGC CTCCTGGGCT CCGAAATAAA 
AAGGGAAAGG AGGAGAAATC GAAAGGGAAA GCCAAGAATA AACCGTCTGC TACAGACAGT 
GAAGGGGAAG ATGATGAGGA CATGACTAAA GAAAAGGAGC CTCCCAGGCC AGGGAAGGAC 
AAAGACAAAA AGGGAGCTGA GCAGGGTTCA GAGGAAGAGA AAGAAGAGAA GGAGGGGGAG 
GTGAAGGCGA ATGATCCCTA TGCCCACCTT AGCAAAAAGG AAAAGAAAAA GCTGAAGAAA 
CAGATGGATT ATGAACGCCA GGTGGAATCA TTAAAGGCAG CTAATGCTGC AGAAAATGAC 
TTCTCTGTGT CCCAGGCAGA GGTGTCTTCC CGCCAGGCAA TGTTAGAAAA TGCATCTGAC 
ATTAAGTTGG AAAAGTTCAG CATCTCGGCC CACGGCAAGG AGCTGTTTGT CAATGCTGAC 
CTGTACATCG TGGCTGGCCG CCGCTATGGG CTGGTGGGGC CCAACGGCAA GGGCAAGACC 
ACACTTCTGA AGCACATTGC CAACCGTGCC CTGAGTATCC CCCCTAACAT TGATGTGCTG 
CTGTGCGAGC AGGAGGTGGT GGCTGATGAA ACACCAGCCG TGCAAGCTGT TCTTCGTGCA 
GACACAAAGC GACTGAGGTT GCTAGAGGAG GAGAAACGGC TTCAGGGACA GCTGGAGCAG 
GGGGATGATA CTGCCGCTGA GAAACTAGAA AAGGTATATG AGGAACTGCG AGCTACTGGG 
GCAGCAGCTG CAGAGGCCAA GGCACGGCGG ATCCTGGCTG GCTTGGGCTT TGACCCTGAG 
ATGCAGAATC GGCCCACACA GAAGTTCTCT GGGGGTTGGC GAATGCGTGT CTCCCTGGCC 
AGGGCACTGT TCATGGAACC CACACTGCTG ATGCTGGACG AGCCTACCAA TCACCTGGAC 
CTCAATGCCG TCATCTGGCT CAATAACTAC CTTCAGGGCT GGAGGAAGAC ACTGCTGATC 
GTCTCCCACG ACCAGGGCTT TCTGGATGAT GTCTGCACCG ATATCATCCA CCTGGACACT 
CAGCGGCTCC ATTACTACAG GGGCAATTAC ATGACCTTCA AGAAGATGTA CCAGCAGAAG 
CAGAAGGAGC TGCTGAAGCA GTACGAGAAA CAGGAGAAGA AGCTGAAGGA GCTGAAGGCT 
GGGGGCAAGT CCACCAAGCA AGCGGAAAAG CAAACAAAGG AAGTCCTGAC TCGAAAACAG 
CAGAAATGCC GAAGGAAAAA CCAGGATGAG GAGTCTCAGG ATCCCCCTGA GCTTCTGAAG 
CGCCCCAGGG AGTACACTGT GCGATTCACC TTCCCAGACC CCCCACCTCT CAGCCCACCT 
GTCCTGGGGC TGCATGGTGT GACGTTTGGC TACGAGGGGC AGAAGCCACT CTTTAAGAAC 
CTGGATTTCG GCATCGACAT GGACTCCCGA ATTTGCATTG TGGGTCCCAA TGGTGTGGGG 
AAGAGCACAC TACTCCTGTT GCTGACTGGC AAGCTGACAC CGACCAACGG GGAAATGAGG 
AAGAACCATC GGCTGAAAAT CGGCTTCTTT AACCAGCAGT ATGCAGAGCA GCTGCACATG 
GAGGAGACGC CCACCGAGTA CCTGCAGCGG GGCTTCAACC TGCCCTATCA GGATGCCCGG 
AAGTGCTTGG GCCGCTTCGG CCTGGAGAGC CACGCCCACA CCATCCAGAT CTGCAAACTC 
TCGGGCGGGC AGAAAGCCCG AGTTGTGTTT GCGGAGCTGG CCTGTCGGGA GCCTGATGTC 
CTCATCTTGG ATGAACCAAC CAATAACTTG GACATAGAGT CCATCGATGC CCTGGGAGAG 
GCCATCAACG AGTACAAGGG AGCTGTGATC GTTGTCAGCC ATGATGCACG CCTCATCACA 
GAAACCAACT GCCAGTTGTG GGTCGTGGAG GAGCAGAGTG TCAGTCAAAT TGATGGTGAC 
TTTGATGACT ACAAGCGAGA GGTGTTGGAG GCCCTGGGTG AAGTCATGGT CAACCGACCT 
CGGGATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017457272.1
CDS	311..2539
Translation	MERLKQLSVPASDEEDEVPVPVPRGRKKAKGGNVFEALIQDESEEEKEEEEEKPVLKPAKPEKNRINKAVAEEPPGLRNKKGKEEKSKGKAKNKPSATDSEGEDDEDMTKEKEPPRPGKDKDKKGAEQGSEEEKEEKEGEVKANDPYAHLSKKEKKKLKKQMDYERQVESLKAANAAENDFSVSQAEVSSRQAMLENASDIKLEKFSISAHGKELFVNADLYIVAGRRYGLVGPNGKGKTTLLKHIANRALSIPPNIDVLLCEQEVVADETPAVQAVLRADTKRLRLLEEEKRLQGQLEQGDDTAAEKLEKVYEELRATGAAAAEAKARRILAGLGFDPEMQNRPTQKFSGGWRMRVSLARALFMEPTLLMLDEPTNHLDLNAVIWLNNYLQGWRKTLLIVSHDQGFLDDVCTDIIHLDTQRLHYYRGNYMTFKKMYQQKQKELLKQYEKQEKKLKELKAGGKSTKQAEKQTKEVLTRKQQKCRRKNQDEESQDPPELLKRPREYTVRFTFPDPPPLSPPVLGLHGVTFGYEGQKPLFKNLDFGIDMDSRICIVGPNGVGKSTLLLLLTGKLTPTNGEMRKNHRLKIGFFNQQYAEQLHMEETPTEYLQRGFNLPYQDARKCLGRFGLESHAHTIQICKLSGGQKARVVFAELACREPDVLILDEPTNNLDIESIDALGEAINEYKGAVIVVSHDARLITETNCQLWVVEEQSVSQIDGDFDDYKREVLEALGEVMVNRPRD

Target ORF information:

RefSeq Version	XM_017601783.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ATP binding cassette subfamily F member 1 (Abcf1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017601783.1

ORF Insert Sequence:

ATGGAGCGCC TTAAGCAGCT GTCTGTGCCA GCCAGTGATG AGGAAGATGA GGTACCTGTC 
CCCGTGCCCC GAGGACGGAA GAAGGCAAAG GGCGGAAATG TTTTTGAAGC CCTGATTCAG 
GATGAAAGTG AGGAGGAAAA AGAAGAGGAG GAAGAAAAGC CTGTTCTCAA GCCTGCCAAG 
CCAGAGAAGA ATCGCATCAA TAAGGCCGTG GCTGAGGAGC CTCCTGGGCT CCGAAATAAA 
AAGGGAAAGG AGGAGAAATC GAAAGGGAAA GCCAAGAATA AACCGTCTGC TACAGACAGT 
GAAGGGGAAG ATGATGAGGA CATGACTAAA GAAAAGGAGC CTCCCAGGCC AGGGAAGGAC 
AAAGACAAAA AGGGAGCTGA GCAGGGTTCA GAGGAAGAGA AAGAAGAGAA GGAGGGGGAG 
GTGAAGGCGA ATGATCCCTA TGCCCACCTT AGCAAAAAGG AAAAGAAAAA GCTGAAGAAA 
CAGATGGATT ATGAACGCCA GGTGGAATCA TTAAAGGCAG CTAATGCTGC AGAAAATGAC 
TTCTCTGTGT CCCAGGCAGA GGTGTCTTCC CGCCAGGCAA TGTTAGAAAA TGCATCTGAC 
ATTAAGTTGG AAAAGTTCAG CATCTCGGCC CACGGCAAGG AGCTGTTTGT CAATGCTGAC 
CTGTACATCG TGGCTGGCCG CCGCTATGGG CTGGTGGGGC CCAACGGCAA GGGCAAGACC 
ACACTTCTGA AGCACATTGC CAACCGTGCC CTGAGTATCC CCCCTAACAT TGATGTGCTG 
CTGTGCGAGC AGGAGGTGGT GGCTGATGAA ACACCAGCCG TGCAAGCTGT TCTTCGTGCA 
GACACAAAGC GACTGAGGTT GCTAGAGGAG GAGAAACGGC TTCAGGGACA GCTGGAGCAG 
GGGGATGATA CTGCCGCTGA GAAACTAGAA AAGGTATATG AGGAACTGCG AGCTACTGGG 
GCAGCAGCTG CAGAGGCCAA GGCACGGCGG ATCCTGGCTG GCTTGGGCTT TGACCCTGAG 
ATGCAGAATC GGCCCACACA GAAGTTCTCT GGGGGTTGGC GAATGCGTGT CTCCCTGGCC 
AGGGCACTGT TCATGGAACC CACACTGCTG ATGCTGGACG AGCCTACCAA TCACCTGGAC 
CTCAATGCCG TCATCTGGCT CAATAACTAC CTTCAGGGCT GGAGGAAGAC ACTGCTGATC 
GTCTCCCACG ACCAGGGCTT TCTGGATGAT GTCTGCACCG ATATCATCCA CCTGGACACT 
CAGCGGCTCC ATTACTACAG GGGCAATTAC ATGACCTTCA AGAAGATGTA CCAGCAGAAG 
CAGAAGGAGC TGCTGAAGCA GTACGAGAAA CAGGAGAAGA AGCTGAAGGA GCTGAAGGCT 
GGGGGCAAGT CCACCAAGCA AGCGGAAAAG CAAACAAAGG AAGTCCTGAC TCGAAAACAG 
CAGAAATGCC GAAGGAAAAA CCAGGATGAG GAGTCTCAGG ATCCCCCTGA GCTTCTGAAG 
CGCCCCAGGG AGTACACTGT GCGATTCACC TTCCCAGACC CCCCACCTCT CAGCCCACCT 
GTCCTGGGGC TGCATGGTGT GACGTTTGGC TACGAGGGGC AGAAGCCACT CTTTAAGAAC 
CTGGATTTCG GCATCGACAT GGACTCCCGA ATTTGCATTG TGGGTCCCAA TGGTGTGGGG 
AAGAGCACAC TACTCCTGTT GCTGACTGGC AAGCTGACAC CGACCAACGG GGAAATGAGG 
AAGAACCATC GGCTGAAAAT CGGCTTCTTT AACCAGCAGT ATGCAGAGCA GCTGCACATG 
GAGGAGACGC CCACCGAGTA CCTGCAGCGG GGCTTCAACC TGCCCTATCA GGATGCCCGG 
AAGTGCTTGG GCCGCTTCGG CCTGGAGAGC CACGCCCACA CCATCCAGAT CTGCAAACTC 
TCGGGCGGGC AGAAAGCCCG AGTTGTGTTT GCGGAGCTGG CCTGTCGGGA GCCTGATGTC 
CTCATCTTGG ATGAACCAAC CAATAACTTG GACATAGAGT CCATCGATGC CCTGGGAGAG 
GCCATCAACG AGTACAAGGG AGCTGTGATC GTTGTCAGCC ATGATGCACG CCTCATCACA 
GAAACCAACT GCCAGTTGTG GGTCGTGGAG GAGCAGAGTG TCAGTCAAAT TGATGGTGAC 
TTTGATGACT ACAAGCGAGA GGTGTTGGAG GCCCTGGGTG AAGTCATGGT CAACCGACCT 
CGGGATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Cell149(6)1393-406(2012 Jun)
Castello A,Fischer B,Eichelbaum K,Horos R,Beckmann BM,Strein C,Davey NE,Humphreys DT,Preiss T,Steinmetz LM,Krijgsveld J,Hentze MW

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Molecular cell46(5)674-90(2012 Jun)
Baltz AG,Munschauer M,Schwanh?usser B,Vasile A,Murakawa Y,Schueler M,Youngs N,Penfold-Brown D,Drew K,Milek M,Wyler E,Bonneau R,Selbach M,Dieterich C,Landthaler M

Defining the membrane proteome of NK cells.
Journal of mass spectrometry : JMS45(1)1-25(2010 Jan)
Ghosh D,Lippert D,Krokhin O,Cortens JP,Wilkins JA

ABC50 promotes translation initiation in mammalian cells.
The Journal of biological chemistry284(36)24061-73(2009 Sep)
Paytubi S,Wang X,Lam YW,Izquierdo L,Hunter MJ,Jan E,Hundal HS,Proud CG

The N-terminal region of ABC50 interacts with eukaryotic initiation factor eIF2 and is a target for regulatory phosphorylation by CK2.
The Biochemical journal409(1)223-31(2008 Jan)
Paytubi S,Morrice NA,Boudeau J,Proud CG