Lyst cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Lyst
Entrez Gene ID	85419
Full Name	lysosomal trafficking regulator
Synonyms	Beige
General protein information	Preferred Names lysosomal trafficking regulator Names lysosomal-trafficking regulator
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 17 Map Location: 17q12.3
Summary	gene deletion is associated with the beige mutant, a model for human Chediak-Higashi syndrome [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_053518.1	NP_445970.1	lysosomal-trafficking regulator

Pathways from BioSystems

Homologies

	LOC718051	XP_002808311.1
Canis lupus familiaris (dog)	LYST	XP_005618856.1
Rattus norvegicus (Norway rat)	Lyst	NP_445970.1
Danio rerio (zebrafish)	lyst	XP_001919653.2
Bos taurus (cattle)	LYST	NP_776445.1
Gallus gallus (chicken)	LYST	XP_419558.4
Homo sapiens (human)	LYST	NP_000072.2
Mus musculus (house mouse)	Lyst	NP_034878.2
Xenopus tropicalis (tropical clawed frog)	lyst	XP_002935877.2
Pan troglodytes (chimpanzee)	LYST	XP_514288.2

Gene Ontology
Bibliography

Related articles in PubMed

Grey, a novel mutation in the murine Lyst gene, causes the beige phenotype by skipping of exon 25.
Runkel F, B?ssow H, Seburn KL, Cox GA, Ward DM, Kaplan J, Franz T
Mammalian genome : official journal of the International Mammalian Genome Society17(3)203-10(2006 Mar)

Histological study of granulated metrial gland (GMG) cells in beige (DA-bg/bg) rats.
Hondo E, Kokubu K, Nagahisa A, Nakamuta N, Ozaki K, Sagara E, Kiso Y
The Journal of reproduction and development51(6)799-803(2005 Dec)

A new beige mutant rat ACI/N-Lystbg-Kyo.
Mori M, Yamasaki K, Nakanishi S, Kitada K, Higuchi K, Namiki C, Hamada S, Serikawa T
Experimental animals52(1)31-6(2003 Jan)

The rat lysosomal trafficking regulator (Lyst) gene is mapped on the telomeric region of chromosome 17.
Masui N, Nishikawa T, Takagi Y, Mori M, Suzuki T, Sato K
Experimental animals52(1)89-91(2003 Jan)

Slate--a new coat color mutant in the mouse.
PIERRO LJ, CHASE HB
The Journal of heredity5447-50(1963 Mar-Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The size of intracellular granules in the granulated metrial gland cells of the beige rats was larger than for the two controls on each corresponding day of pregnancy.
Title: Histological study of granulated metrial gland (GMG) cells in beige (DA-bg/bg) rats.

The following Lyst gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Lyst cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa09778	NM_053518.1 Latest version!	Rattus norvegicus lysosomal trafficking regulator (Lyst), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Lyst ( NM_053518.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_445970.1 Rattus norvegicus lysosomal trafficking regulator (Lyst), mRNA.

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CloneID	ORa09778
Clone ID Related Accession (Same CDS sequence)	NM_053518.1
Accession Version	NM_053518.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 11367bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-30
Organism	Rattus norvegicus(Norway rat)
Product	lysosomal-trafficking regulator
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AB020019.1. On Jan 12, 2005 this sequence version replaced XM_346503.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB020019.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGAGCACGG ACAGCAACTC ATTGGCACGT GAGTTTCTGA TCGATGTCAA CCAGCTTTGC 
AATGCAGTGG TCCAGAGGGC AGAAGCCAGG GAAGAAGAAG AGGAGGAGAC ACATATGGCA 
ACTCTTGGAC AGTACCTTGT CCATGGACGA GGATTTCTGT TACTTACCAA ACTAAATTCT 
ATCATTTATC AGGCCCTGAC ATGCAGAGAA GAACTCCTGA CTCTGCTTCT GTCTCTCCTT 
CCCTTGGTGT GGAAGATACC TGTCCAGGAA CATCAGGCAA CAGATTTTTA CCTGCCATTG 
TCATCTGATA TAATCCTGTC CAAAGAAAAG AACTCAAGTT TGCAAAAATC AACTCAGGGA 
AAATTATATT TAGAAGGAAG TGCTCCAGCT AGTCAAGTTT CTGCATTAGT AAACCTTTTT 
CGAAAAATCA GGCGCCAGCG TAAAAGTACC CATCGCTATT CTGAAAGAGA TGCAAGAAAG 
ACACAGCTCT CCACCTCTGA CTCCGAAGGC AACTCAGATG AAAGAAATAC AGTTGTGCGT 
AAACACAGGA GGTTCCACAC GCTGCCACGG CTCTTGACAG AGTCTCCTAA GGAAGACCAC 
TTTGTAGCCA AACTTGACCC CTCTGCAACC AAAGAACAGG TCCTTTCTGA CACCATGTCT 
TTGGAAAACT CCAGAGAAGT CATTCTGAGA CAGGATTCAA ATGGGGACAT ACTAAGTGAG 
CCAGCTGCCT TGTCTATTCT CAGTAACATG AATAATTCTC CCTTTGACTT GTGTCATGTT 
TTGTTATCTC TATTGGAAAA AGTTTGTAAG TTTGACATTG CTTTGAATCA TAATTCTTCC 
CTAGCACTCA GTGTAGTACC CACACTGACT GAGTTCCTAG CAGGCTTTGG GGACTGCTGT 
AACCAGAGTG ACACTTTGGA AAGACAACTG GTTTCTGCAG GTTGGACCGA AGAGCCGGTA 
GCTTTGGTTC AACGGATGCT CTTTCGCACT GTGCTGCATC TTATGTCAGT AGACGTTAGC 
ACTGCAGAGG CAATGCCAGA AAGTCTTAGG AAAAATCTGA CTGAATTGCT TAGGGCAGCT 
TTAAAAATTA GAGCTTGCTT GGAAAAGCAG CCTGACCCTT TCTCACCGAG ACAAAAGAAA 
ACACTACAGG AGATCCAGGA GGGCTTTGTA TTTTCCAAGT ATCTCCATAG AGCCCTTCTT 
CTACCTGAGC TTCTGGAAGG AGTGCTACAG CTCCTCATCT CTTGTCTTCA GAGTGCAGCT 
TCAAATCCCT TTTACTTCAG TCAAGCCATG GATTTAGTTC AAGAATTTAT CCAGCACCAG 
GGATTTAATC TGTTTGAAAC AGCAGTTCTT CAGATGGAGT GGCTGCTTTC AAGGGATGCT 
GTTCCTTCAG AGGCCGCAGA ACATTTGAAA GCCGTGATAA ACAGTGTAAT AAAAATAATG 
AGTACTGTGA AAAAGGTGAA ATCAGAGCAA CTTCATCATT CCATGTGCAC AAGGAAAAGG 
CACCGGCGTT GTGAGTATTC CCACTTCATG CAGCACCACC GAGATCTCTC AGGTCTCCTG 
GTTTCAGCTT TTAAAAATCA GCTTTCTGAA AGCCCTTTTG AAGAGACTGC TGAGGGAGAT 
GTGCAGTATC CAGAACGGTG CTGCTGCATC GCAGTGTGCG CTCACCAGTG CTTGCGCTTG 
CTCCAGCAGG TTTCTCTGCA AACCACATGT ATCCAGATCC TATCAGGTGT ACACAGTGTT 
GGAATCTGTT GTTGTATGGA TCCTAAATCT GTGATTGTCC CTTTACTCCA TGCTTTTAAA 
TTGCCAGCAC TGAAAAATTT TCAGCAGCAT ATACTGAATG TCCTTAGCAA ACTCCTTTTG 
GATCAGTTAG GAGGAGCAGA GATATCACAG CAAATTAAAA AAGCCGCTTG CAACATCTGT 
ACTGTGGACT CTGACCAACT GGCTAAGTTA GGGGAGACAC TGCAAGGCAC CTTGTGTGGT 
GCTGGTCCTT CCTGTGGCTT GCCCAGTCCT TCTTACAGAT TTCAAGGGAT CCTGCCAAGC 
AGCGGGTCTG AAGACTTCTT GTGGAAATGG GATGCTTTAG AGGCTTACCA GAACTTTATC 
TTTCAAGAAG ACAGATTACA TAACACTCAG ATTGCAAGTC ACATTTGCAA TTTAATCCAG 
AAAGGCAATG TAATTGTTCA GTGGAAATTG TATAATTATA TCTTTAATCC TGTGCTCCAA 
AGAGGAGTTG AATTAGTACA TCATTGTCAA CAGCTAAGCA TTACTTCAGC TCAGACTCAT 
ATGTCTAGCC AACTGAAACA ATATTTGCCT CAGGAAGTGC TTCAAATTTA TTTAAAAACT 
CTGCCCATCC TACTTAAATC CAGGGTAATA AGAGATTTGT TTTTAAGTTG TAATGGAGTA 
AATCACATAA TTGAATTAAA TTACTTAGAT GGGATTCGAA GTCATTCTCT GAAAGCATTT 
GAAACTCTGA TTGTCAGCCT AGGGGAACAA CAGAAACGTG CTGCAGTTCC AGGCGTCGAT 
GGGTTAGACA TCCAGCAGGA GTTGTCATCC TTAAGTGTGG GTCCTTCTCT CCACAAGCAG 
CAAGCTTCTA CAGATTCTCC TTGCAGTCTC AGGAAATTTT ATGCCAGCCT CAGAGACACT 
GATCCAAAAA AACGGAAGAC TGTTCACCAG GATGCTCACA TAAACACCAT AAACCTCTTC 
CTCTGTGTGG CTTTTCTATG TGTCAGTAAA GAAGCGGACT CTGATAGGGA GTCTGCCAAT 
GAGTCAGAAG ATACTTCTGG CTATGACAGT ACTGCCAGTG AGCCATTAAG TCACATGCTG 
CCACGTCTGT CACTTGAGAA TGTTGTCTTA CCTTCCCCTG AATGTTTGCA CCACGCAGCA 
GACATTTGGT CCATGTGTCG TTGGATCTAC ATGTTGAACT CAGTCTTCCA GAAACAATTT 
CACAGACTTG GTGGTTTCCA AGTGTGCCAT GAGTTAATAT TTATGATAAT CCAAAAACTG 
TTCAGAAGTC ATACAGAGGA TCAAGGAAGA AGGCAGGGAG AAATGAGTGT AAACAAAAAC 
CAAGGGTTAA TGAGAATATC TCAACCTGAG ATGATATTGA AGGAAGATGT ATCATCTTCA 
ACAGCACCAG AACCAGGATT TCTGAAAAAG AGTGCTGACA GAGTGAGTGA ATTAGAGTCA 
CAGCATATGC TTCCCACAAG TGCAGAGCAG ATTTTGGCTA CTAAATCCAT TCCGGGGGAA 
GCAAAGACAT TTATGAATCA AGAAAGTGAG ACCTGTCTCC AGAGCATAAG ACTTTTGGAG 
TCTCTCCTGG CCATTTGTCT TCACAGTGCC AGAGCCAGTC AACAGAAGAT GGAATTAGAG 
CTGCCTTCTC AGAGCTTGTC TCTGGAAAAT ATATTGTGTG AACTGAGGGA CCACCTTTCC 
CAGTCAAAGG TGGCAGAAAC AGAATTAGCA AAGCCTTTAT TTGATGCCCT GCTTCGAGTA 
GCCCTGGGGA ATCATTCAGC AGATTTGGAT CCTGGTGATA CTGTGACTGA AAAGAGTCAT 
CCCTCTGAAG AAGTGTTGTC CCAGCCTGGA GATTTTTCAG AAGAAGCTGA GGATTCTCAG 
TGTTGTAGTT TGAAACTTCT GGGTGAGGAA GAAGGTTATG AAGCAGATAG TGAAAGCAAT 
CCTGAGGATG GTGAGACCCA AGATGATGGA GTAGAATTAC CTGAAGCAGA AGGTTTCAGT 
GGATCAATTG TTCCAAACAA CTTACTTGAA AGCCTCACTC ATGGGGAAAT AATATACCCT 
GAGATTTGCA TTCTGGGATT AAATTTGCTT TCTGGTAGCA AAGCTAAACT TGATGTGCTT 
GCTCATGTGT TTGAGAGCTT TCTGAAAATT GCCAGGCAGA AGGAAAAAAA CATTTCTCTG 
CTCATGCAAC AGGGAACTGT GAAAATCCTT CTAGGAGGGT TCTTGAATGT TTTAACACAA 
ACTAACTCTG ATTTCCAAGC ATGCCAGAGA GTATTGGTGG ATCTCTTGGT ATCTTTGATG 
AGTTCAAGAA TGTCCTCAGA AGACTTAACC CTTCTTTTGA GAATATTTCT GGAGAAATCT 
CCTTGTACAG AAATTCTTCT CCATGGTATT CAGAAAATTG TTGAAAGTGA TTTTACTATG 
AGCCCTTCAC AGTATCTGAC CTTCCCTTTG CTGCATACCC CAAGTTTAAG CAATGGTGTC 
TCATCACAAA AGCCTCCTGG GATTCTTAAC AGTAAAGCCT TAGGCTTGCT GAGAAGAGCA 
CGGATTTCCC GAGGCAAGAA AGAGGCTGAT AGAGAGAGTT TTCCCTATAG GCTGCTTTCT 
TCCTGGGACA TAGCCCCAAT CCACTTGCCA TGGCTGGGGC AGAACTGCTG GCCACACCTG 
TCAGAAGGAT TTAGTGTTTC TCTGTGGTTT AATGTGGAAT ATATCCATGA ATCCGAGAGT 
GCTGCCGAAA GAGGAAAGAA AGTAAAGAAA AGAAACAAAC CATCAGTTCT AGAAGACAGC 
AGTTTTGAAA GAGCAGAAGG TGATAGACCA GAAGTTACAG AATCCATCAG TCCTAGTGAC 
AGACTCATAG AAGATGGCTG TATTCACTTG ATTTCATTGG GATCCAAAGC ATTGATGATC 
CAAGTGTGGG CTGATCCTCA TAGTGGCACT TTTATCTTTC GTGTGTGCGT GGATTCAAAT 
GATGATATGA AAGCTGTTAC ATTAGCACAG GTGGAATCAC AGGAGAACAT TTTCTTTCCA 
AGCTCATGGC AGCACTTAAT ACTTACCTAT ATTCAGCATC CTCAAGGGAA AAAGAATGTC 
CATGGGGAAA TCTCGATATG GGTGTCTGGG CAGAGGAAGA CTGATGTCAT CTTGGATTTT 
GTACTACCAA GAAAAACAAG CTTATCATCA GACAGCAATA AAACGTTTTG CATGATTGGC 
CATTGCTTAA CATCCCAAGA AGAGTTTCTT CAATTAGCTG GAAAATGGGA CCTTGGCAAC 
TTGCTCCTCT TCAATGGAGC TAAAATTGGC TCACAGGAGG CCTTTTACCT GTATGCTTGT 
GGACCCAACT ACACATCCAT AATGCCGTGT AAATATGGCA AGCCAGTGGT TGACTACTCC 
AAATATATTA ATAAAGAAAT TTTGAGATGT GATGAAATCA GAGACCTTTT TATGACTAAG 
AAAGATGTGG ATGTTGGACT CTTAATTGAA AGTCTTTCAG TTGTTTATAC AACTTACTGT 
CCTGCTCAGT ACACCATCTA TGAACCTGTG ATTCGACTCA AGGGCCAAGT GAAAACTCAG 
CTGTCTCAAA GACCCTTCAG TTCAAAGGAA GCCCAGAGCA TCTTGCTAGA ACCTTCTCAA 
CTCAAAAGCC TCCAGCCTAC AGAATGTAAA ACCATCCAGG GCATTCTGCA TGAGATTGGT 
GGGGCTGGTA TATTTGTTTT TCTCTTTGCT AGGGTTGTTG AACTTAGTAG CTGTGAAGAA 
ACTCAAGCAT TAGCACTGCG GGTTATACTG TCTTTAATTA AGTACAGCCA ACAGAGAACA 
CATGAACTGG AAAATTGTAA TGGACTTTCT ATGATTCACC AAGTATTGGT CAAACAGAAA 
TGCGTTGTTG GCTTTCACAT TTTAAAGACC CTTCTTGAAG GTTGCTGTGG TGAAGAAGTT 
ATCTTCGTCA ATGAGCATGG AGAGTTCAAG TTGGATGTTG AATCTCATGC TATAATCCAA 
GATGTTAAAC TGTTGCAGGA ACTGTTGCTT GACTGGAAGA TATGGAATAA GGCAGAGCAA 
GGCGTGTGGG AGACTCTGCT GGCAGCTCTG GAAGTCCTCA TCCGGGTAGA GCACCAGCAG 
CAGCAGTTTA ATATTACGCA GTTGCTGAAC GCCCGTGTCG TTCACCACTT CCTACTGACC 
TGTCAGGTTT TACAGGAACA CAGAGAGGGG CAGCTTACAT CAATGCCCCG AGAAGTTTGC 
AGATCGTTTG TGAAAATTAT TGCAGAAGTC CTTGGTTCTC CTCCAGACTT GGAATTATTG 
TCAGTTATCT TCAATTTCCT GTTAGCAGTA CACCCTCCTA CTAATACTTA TGTTTGTCAC 
AACCCTGCAA ACTTCAACTT CTCTTTGCAC ATAGATGGCA AGATCTTTCA TGAGAAAGTG 
CGGTCAATCA TGTACCTGAG GCATTCTAGC AGTGGAGGGC AATCCTTTCC CAGCCCTGGG 
TTTATGGTAA TAAGCCCGTC TGCCTTTACT GCATCTCCTC CTGAAGGAAC CAGTTCTTCC 
AATATTGTTC CACAGAGGAT GGCTGCCCAG ATGGTACGAT CTAGAAGTCT ACCAGCATTT 
CCTACTTATT CACCACTAAT GCAAGCACAA AAACTGGCGG GAAGTTTGGT CTTTAGTGTT 
GACAAGTTAC AAAATATTGC AGATGCCAGC CCAGAGAAAC GGAATCCTTT AGGCAGTCCC 
TACACATTGA AAAAAAGCAA AGAGGAAGCA TTCATTAGTA GCTGTGAGTC TGCAAAAACT 
GTTTGTGAAA TGGAGGCATT TCTTGCAGCC CAGGCCTCTG CCAATGGGGT CTCCAGAGGT 
TCACTGAGGT TTCCCATGGC CAGAATAGAT CACAAAGACT GGGGCACTGA GCACAGGTCA 
TATGATGACA GTCCTGGGGA TGAGTCTTAC CCACGTCGGC CTGACGACCT CAAGGGACTG 
GCCTCATTCC AGCGAAGCCA CAGCACTATT GCGAGCCTTG GACTTGCATT TCCCTCCCAG 
AATGGATCTG CAGTTGCTAG CAGATGGCCG AGTCTTGTTG ATAGGAATGC TGATGATTGG 
GAAAACTTTA CCTTTTCTCC TGCTTATGAG GCAAACTACA ACCGAGCTAC AAGCACCCAC 
AGTGCCATTG AAGACTGTTT GATACCTATC TGCTGTGGAT TTTATGAACT CCTAAGTGGG 
GTTCTTCTTG TTCTGCCTGA TGCTATGCTT GAAGATGTGA TGGACAGGAT TATTCAAGCA 
GATATTCTTC TAGTCCTTGT TAACCACCCA TCACCTGCTA TCCAGCAAGG AGTAATTAAA 
CTGTTACAAG CATACATTAA TAGAGCATCA AAGGAACAAA AGGACAAGTT TCTGAAGAAC 
CGTGGCTTTT CCTTACTAGC CAACCAGTTG TATCTTCACA GGGGAACTCA GGAGTTGTTG 
GAGTGCTTCA TTGAAATGTT CTTTGGTCGG CCATTTGGCC TGGATGAAGA ATTTGATCTG 
GATGAAATGA AACACATGGA ACTGTTCCAG AAGTGGTCTG TCATTCCAAT TCTGGGACTA 
ATAGAGACCT CTCTCTATGA CAATATACTC TTGCACAATT CTCTTTTACT TCTTCTGCAA 
GTTTTAAACT CTTGTTCCAA GGTAGCAGAC ATGCTGCTAG ACAATGGACT GCTCTATGTG 
TTATGTAATA CAGTAGCAGC CCTGAATGGA TTAGAAAAGA ACATTCCTTT GAATGAATTC 
AGATTGCTCG CATGCGATAT ACAGCAACTT TTCATAGCGG TTACAATCCA CGCTTGCAGT 
TCTTCGGGCT CACAGTATTT TAGAGTTATC GAAGACCCCA TTGTACTTAT TGGAGATCTT 
CATAATAGCA AAAATAAGAG GACACAAAAT ATGGCTTTGG CCCTGCAGCT TAGAGTTCTC 
CAGGCTGCTT TGGAATTTAT AAGGAGCACA GCCAATCACG ACTCTGAAAG TCCCTTAGAT 
GCAGTGCAGT CGCCTTCTGC CTACCACCAT TCAGTGCCTC AGAAGAGGAG AAGCATTGCT 
GGTCCTCGCA AATTCCCCCT GGCTCAGACA GAGTCCCTCC TGATGAAGAT GCGCTCGGTG 
GCCAGTGATG AGCTGCACTC TATGATGCAG AGGAGGATGA GCCAGGAGCA CCCCAGCCAG 
GCCTCAGAGG CAGAGCTCGC CCAGCGGCTG CAGAGGCTCA CCATCTTAGC TGTGAACAGG 
ATCATTTACC AAGAGTTGAA TTCAGATATT ATTGACATTT TGAGAACTCC AGAAAATACA 
TCCCAAAGCA AGACCTCAGT GTCCCAGACT GAAACTTCTG AAGAAGATGT GCATTATGAG 
CAACCTTCTG TATCCAATCC ATTCCAAAAA GAAATGTTCA CATATCTGTT GGATGGTTTC 
AAAATATCTA TTGGTTCAAG TAAAACTGGC ACTTCTAAGC AGCAGTGGAC TAAAATTTTG 
GGGTCTTCTA AAGAAACCTT ACGAGTCCAG CTTGGAAGAT TGCTAGCACA TATTTTGTCA 
CCAACCCACA CTGTACAAGA AAGGAAGCAG ATACTTGAGA TAGTCCATGA ACCAGCTCAT 
CAGGATATAC TTCGTGACTG TCTCAGCCCC TCCCCACAAC ATGGAGCCAA GTTGGTTTTG 
TATTTGTCAG AGCTGATACA TAACCATCAA GATGAGTTAA ATGAAGTAGA AATGGACACA 
GCAGAACTGC TTATGAATGC TCTAAAGTTA TGTGGCCACA AGTGCGTCCC TCCCAGTGCC 
CCTTCCAAAC CAGAGCTCAT TAAGATTATC AAAGAGGAGC AAAAGAAATA TGACAGTGAA 
GAGAGTGTGA GTAAAGTTGC GTGGCAGAAG ACAGTGAGCA ACAACCAGCA GAGTCTCTTC 
CAGAGGCTCG ATTTAAAATC CAAGGATATA TCTAAAATAG CTGCAGATAT CACCCAGGCT 
GTATCTCTTT CCCAAGGACT TGAAAGGAAA AAAGTGATTC AGCACATCAG AGGGATGTAC 
AAAGTCGACC TGAGTGCCAG CAGACACTGG CAGGAACTCA TCCAGCAGCT GACACATGAC 
AGAGCAGTCT GGTATGACCC AATCTACTAT CCAACTTCAT GGCAGTTGGA TCCAACAGAA 
GGGCCAAACC GAGAGAGGAG ACGTTTGCAA AGATGCTATT TAACTATTCC AAATAAGTAT 
CTCCTTAGGG ACAGACAAAA GTCGGAAGGT GTGCTCAAGC CACCACTCTC TTACCTTTTT 
GAAGATAAAA CTCATTCTTC TTTCTCCTCT ACTGTCAAAG ACAAAGCTGC AAGTGAATCC 
AACAGAGTGA ATCGAAGATG TATCAGTGTT GCACCATCTA GAGAGACAGC TGGGGAACTG 
TTGTTAGGTA AATGTGGAAT GTATTTTGTG GAAGACAATG CCTCGGATAC AGTTGAAAGT 
TCGAGCCTTC AAGGGGAGTT AGAACCAGCA TCATTTTCTT GGACGTATGA GGAAATTAAA 
GAAATTCATA GGCGCTGGTG GCAGCTGAGA GATAATGCTG TAGAAATCTT TTTAACAAAT 
GGCAGAACAC TCCTCTTGGC ATTTGACAAT ACCAAGGTTC GTGATGATGT CTACCAGAGC 
ATTCTCACAA ATAACCTTCC AAGTCTTCTG GAGTATGGCA ACATCACTGC TCTGACAAAC 
CTGTGGTACA CTGGACAAAT TACCAATTTT GAATATTTGA CTCATTTAAA CAAGCACGCG 
GGCCGATCCT TCAATGATCT CATGCAGTAT CCGGTGTTCC CCTTCATTCT CTCTGACTAT 
GTTAGTGAAA CTCTTGACCT CAATGATCCA TCTATCTATA GAAACCTATC TAAGCCTATA 
GCTGTGCAGT ATAAAGAAAA AGAAGACCGT TACGTTGACA CATACAAGTA CTTGGAGGAG 
GAGTTCCGCA AAGGAGCCCG AGAGGATGAC CCCATGCCTC CTGTGCAGCC CTACCACTAT 
GGCTCCCACT ACTCCAACAG TGGCACCGTG CTCCATTTCC TGGTCAGAAT GCCTCCTTTC 
ACTAAAATGT TTCTAGCCTA TCAAGATCAG AGTTTTGACA TTCCAGACAG AACTTTTCAT 
TCTACAAACA CAACTTGGCG CCTCTCATCC TTTGAATCCA TGACTGATGT GAAGGAGCTG 
ATTCCCGAGT TTTTCTATCT TCCTGAGTTC TTAGTGAACC GTGAAGGTTT TGACTTCGGT 
GTTCGTCAGA ATGGTGAACG GGTTAACCAC GTCAACCTTC CTCCCTGGGC ACGCAACGAT 
CCTCGGCTGT TCATCCTTAT TCACCGGCAG GCACTAGAAT CTGACCATGT GTCCCAGAAC 
ATCTGTCACT GGATTGACTT AGTGTTTGGC TACAAGCAAA AGGGAAAGGC ATCTGTTCAA 
GCCATCAATG TTTTCCACCC TGCCACATAT TTTGGAATGG ATGTCTCTGC AGTTGAAGAT 
CCAGTGCAGA GACGTGCTTT AGAAACCATG ATAAAAACCT ACGGGCAGAC CCCACGTCAG 
TTGTTCCACA CAGCCCATGC CAGCCGACCT GGAGCCAAGC TCAACATTGA AGGAGAGCTC 
CCAGCAGCTG TTGGCTTGTT AGTACAATTT GCTTTCAGAG AGACCCGAGA ACCAATCAAG 
GAGATCACTT ATCCGAGCCC TTTGTCATGG ATAAAAGGCT TGAAGTGGGG AGAGTACGTA 
GGTTCCCCCA GTGCTCCAGT GCCTGTGGTA TGCTTCAGCC AGCCCCATGG AGAAAGATTC 
GGTTCCCTAC AGGCACTGCC CACCAGAGCC ATCTGTGGTT TATCCCAAAA CTTCTGTCTT 
CTGATGACCT ACAGCAAGGA ACAAGGTGTA AGAAGCATGA ACAACACAGA CATTCAGTGG 
TCTGCTATCC TAAGCTGGGG ATATGCTGAC AACATCTTAC GGTTGAAAAG TAAGCAGAGT 
GAGCCACCAA TCAACTTCAT ACAGAGTTCA CAGCAGCACC AGGTGACCAG TTGTGCCTGG 
GTGCCTGACA GTTGTCAGCT CTTCACTGGG AGCAAGTGTG GTGTCATCAC AGCCTATACC 
AACAGGTTCA CAAGCGGCAC GCCCTCAGAA ATTGAAATGG AGAGTCAGAT GCATCTCTAT 
GGACACACAG AAGAGATAAC CAGCTTATGT GTCTGCAAGC CTTATAGCGT GATGATAAGC 
GTGAGCAGAG ATGGGACCTG CATTGTATGG GACCTGAACA GGCTATGCTA TGTACAAAGT 
TTGGCCGGAC ACAAAAACCC TGTGACGGCT GTCTCTGCCA GTGAAACGTC AGGTGACATT 
GCTACTGTGT GTGACTCAGC TGGAGGGGGC AGTGACCTGA GACTCTGGAC AGTGAATGGG 
GACCTCGTTG GACATGTCCA CTGCAGAGAG ATCATTTGTT CTGTAGCTTT CTCCAACCAG 
CCTGAGGGTG TCTCCATCAA TGTCATTGCG GGAGGATTAG AAAATGGCAT TGTAAGGTTG 
TGGAGCACAT GGGACTTGAA GCCTGTGAGA GAAATTACAT TTCCCAAATC AAACAAGCCC 
ATCATAAGCC TGACATTTTC TTGCGATGGC CACCATTTGT ACACTGCCAA CAGTGACGGG 
ACAGTGATTG CATGGTGCCG GAAGGACCAG CAGCGTGTGA AGCAGCCCAT GTTCTACTCT 
TTCCTCAGCA GCTACGCAGC TGGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_445970.1
CDS	1..11367
Translation	MSTDSNSLAREFLIDVNQLCNAVVQRAEAREEEEEETHMATLGQYLVHGRGFLLLTKLNSIIYQALTCREELLTLLLSLLPLVWKIPVQEHQATDFYLPLSSDIILSKEKNSSLQKSTQGKLYLEGSAPASQVSALVNLFRKIRRQRKSTHRYSERDARKTQLSTSDSEGNSDERNTVVRKHRRFHTLPRLLTESPKEDHFVAKLDPSATKEQVLSDTMSLENSREVILRQDSNGDILSEPAALSILSNMNNSPFDLCHVLLSLLEKVCKFDIALNHNSSLALSVVPTLTEFLAGFGDCCNQSDTLERQLVSAGWTEEPVALVQRMLFRTVLHLMSVDVSTAEAMPESLRKNLTELLRAALKIRACLEKQPDPFSPRQKKTLQEIQEGFVFSKYLHRALLLPELLEGVLQLLISCLQSAASNPFYFSQAMDLVQEFIQHQGFNLFETAVLQMEWLLSRDAVPSEAAEHLKAVINSVIKIMSTVKKVKSEQLHHSMCTRKRHRRCEYSHFMQHHRDLSGLLVSAFKNQLSESPFEETAEGDVQYPERCCCIAVCAHQCLRLLQQVSLQTTCIQILSGVHSVGICCCMDPKSVIVPLLHAFKLPALKNFQQHILNVLSKLLLDQLGGAEISQQIKKAACNICTVDSDQLAKLGETLQGTLCGAGPSCGLPSPSYRFQGILPSSGSEDFLWKWDALEAYQNFIFQEDRLHNTQIASHICNLIQKGNVIVQWKLYNYIFNPVLQRGVELVHHCQQLSITSAQTHMSSQLKQYLPQEVLQIYLKTLPILLKSRVIRDLFLSCNGVNHIIELNYLDGIRSHSLKAFETLIVSLGEQQKRAAVPGVDGLDIQQELSSLSVGPSLHKQQASTDSPCSLRKFYASLRDTDPKKRKTVHQDAHINTINLFLCVAFLCVSKEADSDRESANESEDTSGYDSTASEPLSHMLPRLSLENVVLPSPECLHHAADIWSMCRWIYMLNSVFQKQFHRLGGFQVCHELIFMIIQKLFRSHTEDQGRRQGEMSVNKNQGLMRISQPEMILKEDVSSSTAPEPGFLKKSADRVSELESQHMLPTSAEQILATKSIPGEAKTFMNQESETCLQSIRLLESLLAICLHSARASQQKMELELPSQSLSLENILCELRDHLSQSKVAETELAKPLFDALLRVALGNHSADLDPGDTVTEKSHPSEEVLSQPGDFSEEAEDSQCCSLKLLGEEEGYEADSESNPEDGETQDDGVELPEAEGFSGSIVPNNLLESLTHGEIIYPEICILGLNLLSGSKAKLDVLAHVFESFLKIARQKEKNISLLMQQGTVKILLGGFLNVLTQTNSDFQACQRVLVDLLVSLMSSRMSSEDLTLLLRIFLEKSPCTEILLHGIQKIVESDFTMSPSQYLTFPLLHTPSLSNGVSSQKPPGILNSKALGLLRRARISRGKKEADRESFPYRLLSSWDIAPIHLPWLGQNCWPHLSEGFSVSLWFNVEYIHESESAAERGKKVKKRNKPSVLEDSSFERAEGDRPEVTESISPSDRLIEDGCIHLISLGSKALMIQVWADPHSGTFIFRVCVDSNDDMKAVTLAQVESQENIFFPSSWQHLILTYIQHPQGKKNVHGEISIWVSGQRKTDVILDFVLPRKTSLSSDSNKTFCMIGHCLTSQEEFLQLAGKWDLGNLLLFNGAKIGSQEAFYLYACGPNYTSIMPCKYGKPVVDYSKYINKEILRCDEIRDLFMTKKDVDVGLLIESLSVVYTTYCPAQYTIYEPVIRLKGQVKTQLSQRPFSSKEAQSILLEPSQLKSLQPTECKTIQGILHEIGGAGIFVFLFARVVELSSCEETQALALRVILSLIKYSQQRTHELENCNGLSMIHQVLVKQKCVVGFHILKTLLEGCCGEEVIFVNEHGEFKLDVESHAIIQDVKLLQELLLDWKIWNKAEQGVWETLLAALEVLIRVEHQQQQFNITQLLNARVVHHFLLTCQVLQEHREGQLTSMPREVCRSFVKIIAEVLGSPPDLELLSVIFNFLLAVHPPTNTYVCHNPANFNFSLHIDGKIFHEKVRSIMYLRHSSSGGQSFPSPGFMVISPSAFTASPPEGTSSSNIVPQRMAAQMVRSRSLPAFPTYSPLMQAQKLAGSLVFSVDKLQNIADASPEKRNPLGSPYTLKKSKEEAFISSCESAKTVCEMEAFLAAQASANGVSRGSLRFPMARIDHKDWGTEHRSYDDSPGDESYPRRPDDLKGLASFQRSHSTIASLGLAFPSQNGSAVASRWPSLVDRNADDWENFTFSPAYEANYNRATSTHSAIEDCLIPICCGFYELLSGVLLVLPDAMLEDVMDRIIQADILLVLVNHPSPAIQQGVIKLLQAYINRASKEQKDKFLKNRGFSLLANQLYLHRGTQELLECFIEMFFGRPFGLDEEFDLDEMKHMELFQKWSVIPILGLIETSLYDNILLHNSLLLLLQVLNSCSKVADMLLDNGLLYVLCNTVAALNGLEKNIPLNEFRLLACDIQQLFIAVTIHACSSSGSQYFRVIEDPIVLIGDLHNSKNKRTQNMALALQLRVLQAALEFIRSTANHDSESPLDAVQSPSAYHHSVPQKRRSIAGPRKFPLAQTESLLMKMRSVASDELHSMMQRRMSQEHPSQASEAELAQRLQRLTILAVNRIIYQELNSDIIDILRTPENTSQSKTSVSQTETSEEDVHYEQPSVSNPFQKEMFTYLLDGFKISIGSSKTGTSKQQWTKILGSSKETLRVQLGRLLAHILSPTHTVQERKQILEIVHEPAHQDILRDCLSPSPQHGAKLVLYLSELIHNHQDELNEVEMDTAELLMNALKLCGHKCVPPSAPSKPELIKIIKEEQKKYDSEESVSKVAWQKTVSNNQQSLFQRLDLKSKDISKIAADITQAVSLSQGLERKKVIQHIRGMYKVDLSASRHWQELIQQLTHDRAVWYDPIYYPTSWQLDPTEGPNRERRRLQRCYLTIPNKYLLRDRQKSEGVLKPPLSYLFEDKTHSSFSSTVKDKAASESNRVNRRCISVAPSRETAGELLLGKCGMYFVEDNASDTVESSSLQGELEPASFSWTYEEIKEIHRRWWQLRDNAVEIFLTNGRTLLLAFDNTKVRDDVYQSILTNNLPSLLEYGNITALTNLWYTGQITNFEYLTHLNKHAGRSFNDLMQYPVFPFILSDYVSETLDLNDPSIYRNLSKPIAVQYKEKEDRYVDTYKYLEEEFRKGAREDDPMPPVQPYHYGSHYSNSGTVLHFLVRMPPFTKMFLAYQDQSFDIPDRTFHSTNTTWRLSSFESMTDVKELIPEFFYLPEFLVNREGFDFGVRQNGERVNHVNLPPWARNDPRLFILIHRQALESDHVSQNICHWIDLVFGYKQKGKASVQAINVFHPATYFGMDVSAVEDPVQRRALETMIKTYGQTPRQLFHTAHASRPGAKLNIEGELPAAVGLLVQFAFRETREPIKEITYPSPLSWIKGLKWGEYVGSPSAPVPVVCFSQPHGERFGSLQALPTRAICGLSQNFCLLMTYSKEQGVRSMNNTDIQWSAILSWGYADNILRLKSKQSEPPINFIQSSQQHQVTSCAWVPDSCQLFTGSKCGVITAYTNRFTSGTPSEIEMESQMHLYGHTEEITSLCVCKPYSVMISVSRDGTCIVWDLNRLCYVQSLAGHKNPVTAVSASETSGDIATVCDSAGGGSDLRLWTVNGDLVGHVHCREIICSVAFSNQPEGVSINVIAGGLENGIVRLWSTWDLKPVREITFPKSNKPIISLTFSCDGHHLYTANSDGTVIAWCRKDQQRVKQPMFYSFLSSYAAG

Target ORF information:

RefSeq Version	NM_053518.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus lysosomal trafficking regulator (Lyst), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_053518.1

ORF Insert Sequence:

ATGAGCACGG ACAGCAACTC ATTGGCACGT GAGTTTCTGA TCGATGTCAA CCAGCTTTGC 
AATGCAGTGG TCCAGAGGGC AGAAGCCAGG GAAGAAGAAG AGGAGGAGAC ACATATGGCA 
ACTCTTGGAC AGTACCTTGT CCATGGACGA GGATTTCTGT TACTTACCAA ACTAAATTCT 
ATCATTTATC AGGCCCTGAC ATGCAGAGAA GAACTCCTGA CTCTGCTTCT GTCTCTCCTT 
CCCTTGGTGT GGAAGATACC TGTCCAGGAA CATCAGGCAA CAGATTTTTA CCTGCCATTG 
TCATCTGATA TAATCCTGTC CAAAGAAAAG AACTCAAGTT TGCAAAAATC AACTCAGGGA 
AAATTATATT TAGAAGGAAG TGCTCCAGCT AGTCAAGTTT CTGCATTAGT AAACCTTTTT 
CGAAAAATCA GGCGCCAGCG TAAAAGTACC CATCGCTATT CTGAAAGAGA TGCAAGAAAG 
ACACAGCTCT CCACCTCTGA CTCCGAAGGC AACTCAGATG AAAGAAATAC AGTTGTGCGT 
AAACACAGGA GGTTCCACAC GCTGCCACGG CTCTTGACAG AGTCTCCTAA GGAAGACCAC 
TTTGTAGCCA AACTTGACCC CTCTGCAACC AAAGAACAGG TCCTTTCTGA CACCATGTCT 
TTGGAAAACT CCAGAGAAGT CATTCTGAGA CAGGATTCAA ATGGGGACAT ACTAAGTGAG 
CCAGCTGCCT TGTCTATTCT CAGTAACATG AATAATTCTC CCTTTGACTT GTGTCATGTT 
TTGTTATCTC TATTGGAAAA AGTTTGTAAG TTTGACATTG CTTTGAATCA TAATTCTTCC 
CTAGCACTCA GTGTAGTACC CACACTGACT GAGTTCCTAG CAGGCTTTGG GGACTGCTGT 
AACCAGAGTG ACACTTTGGA AAGACAACTG GTTTCTGCAG GTTGGACCGA AGAGCCGGTA 
GCTTTGGTTC AACGGATGCT CTTTCGCACT GTGCTGCATC TTATGTCAGT AGACGTTAGC 
ACTGCAGAGG CAATGCCAGA AAGTCTTAGG AAAAATCTGA CTGAATTGCT TAGGGCAGCT 
TTAAAAATTA GAGCTTGCTT GGAAAAGCAG CCTGACCCTT TCTCACCGAG ACAAAAGAAA 
ACACTACAGG AGATCCAGGA GGGCTTTGTA TTTTCCAAGT ATCTCCATAG AGCCCTTCTT 
CTACCTGAGC TTCTGGAAGG AGTGCTACAG CTCCTCATCT CTTGTCTTCA GAGTGCAGCT 
TCAAATCCCT TTTACTTCAG TCAAGCCATG GATTTAGTTC AAGAATTTAT CCAGCACCAG 
GGATTTAATC TGTTTGAAAC AGCAGTTCTT CAGATGGAGT GGCTGCTTTC AAGGGATGCT 
GTTCCTTCAG AGGCCGCAGA ACATTTGAAA GCCGTGATAA ACAGTGTAAT AAAAATAATG 
AGTACTGTGA AAAAGGTGAA ATCAGAGCAA CTTCATCATT CCATGTGCAC AAGGAAAAGG 
CACCGGCGTT GTGAGTATTC CCACTTCATG CAGCACCACC GAGATCTCTC AGGTCTCCTG 
GTTTCAGCTT TTAAAAATCA GCTTTCTGAA AGCCCTTTTG AAGAGACTGC TGAGGGAGAT 
GTGCAGTATC CAGAACGGTG CTGCTGCATC GCAGTGTGCG CTCACCAGTG CTTGCGCTTG 
CTCCAGCAGG TTTCTCTGCA AACCACATGT ATCCAGATCC TATCAGGTGT ACACAGTGTT 
GGAATCTGTT GTTGTATGGA TCCTAAATCT GTGATTGTCC CTTTACTCCA TGCTTTTAAA 
TTGCCAGCAC TGAAAAATTT TCAGCAGCAT ATACTGAATG TCCTTAGCAA ACTCCTTTTG 
GATCAGTTAG GAGGAGCAGA GATATCACAG CAAATTAAAA AAGCCGCTTG CAACATCTGT 
ACTGTGGACT CTGACCAACT GGCTAAGTTA GGGGAGACAC TGCAAGGCAC CTTGTGTGGT 
GCTGGTCCTT CCTGTGGCTT GCCCAGTCCT TCTTACAGAT TTCAAGGGAT CCTGCCAAGC 
AGCGGGTCTG AAGACTTCTT GTGGAAATGG GATGCTTTAG AGGCTTACCA GAACTTTATC 
TTTCAAGAAG ACAGATTACA TAACACTCAG ATTGCAAGTC ACATTTGCAA TTTAATCCAG 
AAAGGCAATG TAATTGTTCA GTGGAAATTG TATAATTATA TCTTTAATCC TGTGCTCCAA 
AGAGGAGTTG AATTAGTACA TCATTGTCAA CAGCTAAGCA TTACTTCAGC TCAGACTCAT 
ATGTCTAGCC AACTGAAACA ATATTTGCCT CAGGAAGTGC TTCAAATTTA TTTAAAAACT 
CTGCCCATCC TACTTAAATC CAGGGTAATA AGAGATTTGT TTTTAAGTTG TAATGGAGTA 
AATCACATAA TTGAATTAAA TTACTTAGAT GGGATTCGAA GTCATTCTCT GAAAGCATTT 
GAAACTCTGA TTGTCAGCCT AGGGGAACAA CAGAAACGTG CTGCAGTTCC AGGCGTCGAT 
GGGTTAGACA TCCAGCAGGA GTTGTCATCC TTAAGTGTGG GTCCTTCTCT CCACAAGCAG 
CAAGCTTCTA CAGATTCTCC TTGCAGTCTC AGGAAATTTT ATGCCAGCCT CAGAGACACT 
GATCCAAAAA AACGGAAGAC TGTTCACCAG GATGCTCACA TAAACACCAT AAACCTCTTC 
CTCTGTGTGG CTTTTCTATG TGTCAGTAAA GAAGCGGACT CTGATAGGGA GTCTGCCAAT 
GAGTCAGAAG ATACTTCTGG CTATGACAGT ACTGCCAGTG AGCCATTAAG TCACATGCTG 
CCACGTCTGT CACTTGAGAA TGTTGTCTTA CCTTCCCCTG AATGTTTGCA CCACGCAGCA 
GACATTTGGT CCATGTGTCG TTGGATCTAC ATGTTGAACT CAGTCTTCCA GAAACAATTT 
CACAGACTTG GTGGTTTCCA AGTGTGCCAT GAGTTAATAT TTATGATAAT CCAAAAACTG 
TTCAGAAGTC ATACAGAGGA TCAAGGAAGA AGGCAGGGAG AAATGAGTGT AAACAAAAAC 
CAAGGGTTAA TGAGAATATC TCAACCTGAG ATGATATTGA AGGAAGATGT ATCATCTTCA 
ACAGCACCAG AACCAGGATT TCTGAAAAAG AGTGCTGACA GAGTGAGTGA ATTAGAGTCA 
CAGCATATGC TTCCCACAAG TGCAGAGCAG ATTTTGGCTA CTAAATCCAT TCCGGGGGAA 
GCAAAGACAT TTATGAATCA AGAAAGTGAG ACCTGTCTCC AGAGCATAAG ACTTTTGGAG 
TCTCTCCTGG CCATTTGTCT TCACAGTGCC AGAGCCAGTC AACAGAAGAT GGAATTAGAG 
CTGCCTTCTC AGAGCTTGTC TCTGGAAAAT ATATTGTGTG AACTGAGGGA CCACCTTTCC 
CAGTCAAAGG TGGCAGAAAC AGAATTAGCA AAGCCTTTAT TTGATGCCCT GCTTCGAGTA 
GCCCTGGGGA ATCATTCAGC AGATTTGGAT CCTGGTGATA CTGTGACTGA AAAGAGTCAT 
CCCTCTGAAG AAGTGTTGTC CCAGCCTGGA GATTTTTCAG AAGAAGCTGA GGATTCTCAG 
TGTTGTAGTT TGAAACTTCT GGGTGAGGAA GAAGGTTATG AAGCAGATAG TGAAAGCAAT 
CCTGAGGATG GTGAGACCCA AGATGATGGA GTAGAATTAC CTGAAGCAGA AGGTTTCAGT 
GGATCAATTG TTCCAAACAA CTTACTTGAA AGCCTCACTC ATGGGGAAAT AATATACCCT 
GAGATTTGCA TTCTGGGATT AAATTTGCTT TCTGGTAGCA AAGCTAAACT TGATGTGCTT 
GCTCATGTGT TTGAGAGCTT TCTGAAAATT GCCAGGCAGA AGGAAAAAAA CATTTCTCTG 
CTCATGCAAC AGGGAACTGT GAAAATCCTT CTAGGAGGGT TCTTGAATGT TTTAACACAA 
ACTAACTCTG ATTTCCAAGC ATGCCAGAGA GTATTGGTGG ATCTCTTGGT ATCTTTGATG 
AGTTCAAGAA TGTCCTCAGA AGACTTAACC CTTCTTTTGA GAATATTTCT GGAGAAATCT 
CCTTGTACAG AAATTCTTCT CCATGGTATT CAGAAAATTG TTGAAAGTGA TTTTACTATG 
AGCCCTTCAC AGTATCTGAC CTTCCCTTTG CTGCATACCC CAAGTTTAAG CAATGGTGTC 
TCATCACAAA AGCCTCCTGG GATTCTTAAC AGTAAAGCCT TAGGCTTGCT GAGAAGAGCA 
CGGATTTCCC GAGGCAAGAA AGAGGCTGAT AGAGAGAGTT TTCCCTATAG GCTGCTTTCT 
TCCTGGGACA TAGCCCCAAT CCACTTGCCA TGGCTGGGGC AGAACTGCTG GCCACACCTG 
TCAGAAGGAT TTAGTGTTTC TCTGTGGTTT AATGTGGAAT ATATCCATGA ATCCGAGAGT 
GCTGCCGAAA GAGGAAAGAA AGTAAAGAAA AGAAACAAAC CATCAGTTCT AGAAGACAGC 
AGTTTTGAAA GAGCAGAAGG TGATAGACCA GAAGTTACAG AATCCATCAG TCCTAGTGAC 
AGACTCATAG AAGATGGCTG TATTCACTTG ATTTCATTGG GATCCAAAGC ATTGATGATC 
CAAGTGTGGG CTGATCCTCA TAGTGGCACT TTTATCTTTC GTGTGTGCGT GGATTCAAAT 
GATGATATGA AAGCTGTTAC ATTAGCACAG GTGGAATCAC AGGAGAACAT TTTCTTTCCA 
AGCTCATGGC AGCACTTAAT ACTTACCTAT ATTCAGCATC CTCAAGGGAA AAAGAATGTC 
CATGGGGAAA TCTCGATATG GGTGTCTGGG CAGAGGAAGA CTGATGTCAT CTTGGATTTT 
GTACTACCAA GAAAAACAAG CTTATCATCA GACAGCAATA AAACGTTTTG CATGATTGGC 
CATTGCTTAA CATCCCAAGA AGAGTTTCTT CAATTAGCTG GAAAATGGGA CCTTGGCAAC 
TTGCTCCTCT TCAATGGAGC TAAAATTGGC TCACAGGAGG CCTTTTACCT GTATGCTTGT 
GGACCCAACT ACACATCCAT AATGCCGTGT AAATATGGCA AGCCAGTGGT TGACTACTCC 
AAATATATTA ATAAAGAAAT TTTGAGATGT GATGAAATCA GAGACCTTTT TATGACTAAG 
AAAGATGTGG ATGTTGGACT CTTAATTGAA AGTCTTTCAG TTGTTTATAC AACTTACTGT 
CCTGCTCAGT ACACCATCTA TGAACCTGTG ATTCGACTCA AGGGCCAAGT GAAAACTCAG 
CTGTCTCAAA GACCCTTCAG TTCAAAGGAA GCCCAGAGCA TCTTGCTAGA ACCTTCTCAA 
CTCAAAAGCC TCCAGCCTAC AGAATGTAAA ACCATCCAGG GCATTCTGCA TGAGATTGGT 
GGGGCTGGTA TATTTGTTTT TCTCTTTGCT AGGGTTGTTG AACTTAGTAG CTGTGAAGAA 
ACTCAAGCAT TAGCACTGCG GGTTATACTG TCTTTAATTA AGTACAGCCA ACAGAGAACA 
CATGAACTGG AAAATTGTAA TGGACTTTCT ATGATTCACC AAGTATTGGT CAAACAGAAA 
TGCGTTGTTG GCTTTCACAT TTTAAAGACC CTTCTTGAAG GTTGCTGTGG TGAAGAAGTT 
ATCTTCGTCA ATGAGCATGG AGAGTTCAAG TTGGATGTTG AATCTCATGC TATAATCCAA 
GATGTTAAAC TGTTGCAGGA ACTGTTGCTT GACTGGAAGA TATGGAATAA GGCAGAGCAA 
GGCGTGTGGG AGACTCTGCT GGCAGCTCTG GAAGTCCTCA TCCGGGTAGA GCACCAGCAG 
CAGCAGTTTA ATATTACGCA GTTGCTGAAC GCCCGTGTCG TTCACCACTT CCTACTGACC 
TGTCAGGTTT TACAGGAACA CAGAGAGGGG CAGCTTACAT CAATGCCCCG AGAAGTTTGC 
AGATCGTTTG TGAAAATTAT TGCAGAAGTC CTTGGTTCTC CTCCAGACTT GGAATTATTG 
TCAGTTATCT TCAATTTCCT GTTAGCAGTA CACCCTCCTA CTAATACTTA TGTTTGTCAC 
AACCCTGCAA ACTTCAACTT CTCTTTGCAC ATAGATGGCA AGATCTTTCA TGAGAAAGTG 
CGGTCAATCA TGTACCTGAG GCATTCTAGC AGTGGAGGGC AATCCTTTCC CAGCCCTGGG 
TTTATGGTAA TAAGCCCGTC TGCCTTTACT GCATCTCCTC CTGAAGGAAC CAGTTCTTCC 
AATATTGTTC CACAGAGGAT GGCTGCCCAG ATGGTACGAT CTAGAAGTCT ACCAGCATTT 
CCTACTTATT CACCACTAAT GCAAGCACAA AAACTGGCGG GAAGTTTGGT CTTTAGTGTT 
GACAAGTTAC AAAATATTGC AGATGCCAGC CCAGAGAAAC GGAATCCTTT AGGCAGTCCC 
TACACATTGA AAAAAAGCAA AGAGGAAGCA TTCATTAGTA GCTGTGAGTC TGCAAAAACT 
GTTTGTGAAA TGGAGGCATT TCTTGCAGCC CAGGCCTCTG CCAATGGGGT CTCCAGAGGT 
TCACTGAGGT TTCCCATGGC CAGAATAGAT CACAAAGACT GGGGCACTGA GCACAGGTCA 
TATGATGACA GTCCTGGGGA TGAGTCTTAC CCACGTCGGC CTGACGACCT CAAGGGACTG 
GCCTCATTCC AGCGAAGCCA CAGCACTATT GCGAGCCTTG GACTTGCATT TCCCTCCCAG 
AATGGATCTG CAGTTGCTAG CAGATGGCCG AGTCTTGTTG ATAGGAATGC TGATGATTGG 
GAAAACTTTA CCTTTTCTCC TGCTTATGAG GCAAACTACA ACCGAGCTAC AAGCACCCAC 
AGTGCCATTG AAGACTGTTT GATACCTATC TGCTGTGGAT TTTATGAACT CCTAAGTGGG 
GTTCTTCTTG TTCTGCCTGA TGCTATGCTT GAAGATGTGA TGGACAGGAT TATTCAAGCA 
GATATTCTTC TAGTCCTTGT TAACCACCCA TCACCTGCTA TCCAGCAAGG AGTAATTAAA 
CTGTTACAAG CATACATTAA TAGAGCATCA AAGGAACAAA AGGACAAGTT TCTGAAGAAC 
CGTGGCTTTT CCTTACTAGC CAACCAGTTG TATCTTCACA GGGGAACTCA GGAGTTGTTG 
GAGTGCTTCA TTGAAATGTT CTTTGGTCGG CCATTTGGCC TGGATGAAGA ATTTGATCTG 
GATGAAATGA AACACATGGA ACTGTTCCAG AAGTGGTCTG TCATTCCAAT TCTGGGACTA 
ATAGAGACCT CTCTCTATGA CAATATACTC TTGCACAATT CTCTTTTACT TCTTCTGCAA 
GTTTTAAACT CTTGTTCCAA GGTAGCAGAC ATGCTGCTAG ACAATGGACT GCTCTATGTG 
TTATGTAATA CAGTAGCAGC CCTGAATGGA TTAGAAAAGA ACATTCCTTT GAATGAATTC 
AGATTGCTCG CATGCGATAT ACAGCAACTT TTCATAGCGG TTACAATCCA CGCTTGCAGT 
TCTTCGGGCT CACAGTATTT TAGAGTTATC GAAGACCCCA TTGTACTTAT TGGAGATCTT 
CATAATAGCA AAAATAAGAG GACACAAAAT ATGGCTTTGG CCCTGCAGCT TAGAGTTCTC 
CAGGCTGCTT TGGAATTTAT AAGGAGCACA GCCAATCACG ACTCTGAAAG TCCCTTAGAT 
GCAGTGCAGT CGCCTTCTGC CTACCACCAT TCAGTGCCTC AGAAGAGGAG AAGCATTGCT 
GGTCCTCGCA AATTCCCCCT GGCTCAGACA GAGTCCCTCC TGATGAAGAT GCGCTCGGTG 
GCCAGTGATG AGCTGCACTC TATGATGCAG AGGAGGATGA GCCAGGAGCA CCCCAGCCAG 
GCCTCAGAGG CAGAGCTCGC CCAGCGGCTG CAGAGGCTCA CCATCTTAGC TGTGAACAGG 
ATCATTTACC AAGAGTTGAA TTCAGATATT ATTGACATTT TGAGAACTCC AGAAAATACA 
TCCCAAAGCA AGACCTCAGT GTCCCAGACT GAAACTTCTG AAGAAGATGT GCATTATGAG 
CAACCTTCTG TATCCAATCC ATTCCAAAAA GAAATGTTCA CATATCTGTT GGATGGTTTC 
AAAATATCTA TTGGTTCAAG TAAAACTGGC ACTTCTAAGC AGCAGTGGAC TAAAATTTTG 
GGGTCTTCTA AAGAAACCTT ACGAGTCCAG CTTGGAAGAT TGCTAGCACA TATTTTGTCA 
CCAACCCACA CTGTACAAGA AAGGAAGCAG ATACTTGAGA TAGTCCATGA ACCAGCTCAT 
CAGGATATAC TTCGTGACTG TCTCAGCCCC TCCCCACAAC ATGGAGCCAA GTTGGTTTTG 
TATTTGTCAG AGCTGATACA TAACCATCAA GATGAGTTAA ATGAAGTAGA AATGGACACA 
GCAGAACTGC TTATGAATGC TCTAAAGTTA TGTGGCCACA AGTGCGTCCC TCCCAGTGCC 
CCTTCCAAAC CAGAGCTCAT TAAGATTATC AAAGAGGAGC AAAAGAAATA TGACAGTGAA 
GAGAGTGTGA GTAAAGTTGC GTGGCAGAAG ACAGTGAGCA ACAACCAGCA GAGTCTCTTC 
CAGAGGCTCG ATTTAAAATC CAAGGATATA TCTAAAATAG CTGCAGATAT CACCCAGGCT 
GTATCTCTTT CCCAAGGACT TGAAAGGAAA AAAGTGATTC AGCACATCAG AGGGATGTAC 
AAAGTCGACC TGAGTGCCAG CAGACACTGG CAGGAACTCA TCCAGCAGCT GACACATGAC 
AGAGCAGTCT GGTATGACCC AATCTACTAT CCAACTTCAT GGCAGTTGGA TCCAACAGAA 
GGGCCAAACC GAGAGAGGAG ACGTTTGCAA AGATGCTATT TAACTATTCC AAATAAGTAT 
CTCCTTAGGG ACAGACAAAA GTCGGAAGGT GTGCTCAAGC CACCACTCTC TTACCTTTTT 
GAAGATAAAA CTCATTCTTC TTTCTCCTCT ACTGTCAAAG ACAAAGCTGC AAGTGAATCC 
AACAGAGTGA ATCGAAGATG TATCAGTGTT GCACCATCTA GAGAGACAGC TGGGGAACTG 
TTGTTAGGTA AATGTGGAAT GTATTTTGTG GAAGACAATG CCTCGGATAC AGTTGAAAGT 
TCGAGCCTTC AAGGGGAGTT AGAACCAGCA TCATTTTCTT GGACGTATGA GGAAATTAAA 
GAAATTCATA GGCGCTGGTG GCAGCTGAGA GATAATGCTG TAGAAATCTT TTTAACAAAT 
GGCAGAACAC TCCTCTTGGC ATTTGACAAT ACCAAGGTTC GTGATGATGT CTACCAGAGC 
ATTCTCACAA ATAACCTTCC AAGTCTTCTG GAGTATGGCA ACATCACTGC TCTGACAAAC 
CTGTGGTACA CTGGACAAAT TACCAATTTT GAATATTTGA CTCATTTAAA CAAGCACGCG 
GGCCGATCCT TCAATGATCT CATGCAGTAT CCGGTGTTCC CCTTCATTCT CTCTGACTAT 
GTTAGTGAAA CTCTTGACCT CAATGATCCA TCTATCTATA GAAACCTATC TAAGCCTATA 
GCTGTGCAGT ATAAAGAAAA AGAAGACCGT TACGTTGACA CATACAAGTA CTTGGAGGAG 
GAGTTCCGCA AAGGAGCCCG AGAGGATGAC CCCATGCCTC CTGTGCAGCC CTACCACTAT 
GGCTCCCACT ACTCCAACAG TGGCACCGTG CTCCATTTCC TGGTCAGAAT GCCTCCTTTC 
ACTAAAATGT TTCTAGCCTA TCAAGATCAG AGTTTTGACA TTCCAGACAG AACTTTTCAT 
TCTACAAACA CAACTTGGCG CCTCTCATCC TTTGAATCCA TGACTGATGT GAAGGAGCTG 
ATTCCCGAGT TTTTCTATCT TCCTGAGTTC TTAGTGAACC GTGAAGGTTT TGACTTCGGT 
GTTCGTCAGA ATGGTGAACG GGTTAACCAC GTCAACCTTC CTCCCTGGGC ACGCAACGAT 
CCTCGGCTGT TCATCCTTAT TCACCGGCAG GCACTAGAAT CTGACCATGT GTCCCAGAAC 
ATCTGTCACT GGATTGACTT AGTGTTTGGC TACAAGCAAA AGGGAAAGGC ATCTGTTCAA 
GCCATCAATG TTTTCCACCC TGCCACATAT TTTGGAATGG ATGTCTCTGC AGTTGAAGAT 
CCAGTGCAGA GACGTGCTTT AGAAACCATG ATAAAAACCT ACGGGCAGAC CCCACGTCAG 
TTGTTCCACA CAGCCCATGC CAGCCGACCT GGAGCCAAGC TCAACATTGA AGGAGAGCTC 
CCAGCAGCTG TTGGCTTGTT AGTACAATTT GCTTTCAGAG AGACCCGAGA ACCAATCAAG 
GAGATCACTT ATCCGAGCCC TTTGTCATGG ATAAAAGGCT TGAAGTGGGG AGAGTACGTA 
GGTTCCCCCA GTGCTCCAGT GCCTGTGGTA TGCTTCAGCC AGCCCCATGG AGAAAGATTC 
GGTTCCCTAC AGGCACTGCC CACCAGAGCC ATCTGTGGTT TATCCCAAAA CTTCTGTCTT 
CTGATGACCT ACAGCAAGGA ACAAGGTGTA AGAAGCATGA ACAACACAGA CATTCAGTGG 
TCTGCTATCC TAAGCTGGGG ATATGCTGAC AACATCTTAC GGTTGAAAAG TAAGCAGAGT 
GAGCCACCAA TCAACTTCAT ACAGAGTTCA CAGCAGCACC AGGTGACCAG TTGTGCCTGG 
GTGCCTGACA GTTGTCAGCT CTTCACTGGG AGCAAGTGTG GTGTCATCAC AGCCTATACC 
AACAGGTTCA CAAGCGGCAC GCCCTCAGAA ATTGAAATGG AGAGTCAGAT GCATCTCTAT 
GGACACACAG AAGAGATAAC CAGCTTATGT GTCTGCAAGC CTTATAGCGT GATGATAAGC 
GTGAGCAGAG ATGGGACCTG CATTGTATGG GACCTGAACA GGCTATGCTA TGTACAAAGT 
TTGGCCGGAC ACAAAAACCC TGTGACGGCT GTCTCTGCCA GTGAAACGTC AGGTGACATT 
GCTACTGTGT GTGACTCAGC TGGAGGGGGC AGTGACCTGA GACTCTGGAC AGTGAATGGG 
GACCTCGTTG GACATGTCCA CTGCAGAGAG ATCATTTGTT CTGTAGCTTT CTCCAACCAG 
CCTGAGGGTG TCTCCATCAA TGTCATTGCG GGAGGATTAG AAAATGGCAT TGTAAGGTTG 
TGGAGCACAT GGGACTTGAA GCCTGTGAGA GAAATTACAT TTCCCAAATC AAACAAGCCC 
ATCATAAGCC TGACATTTTC TTGCGATGGC CACCATTTGT ACACTGCCAA CAGTGACGGG 
ACAGTGATTG CATGGTGCCG GAAGGACCAG CAGCGTGTGA AGCAGCCCAT GTTCTACTCT 
TTCCTCAGCA GCTACGCAGC TGGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Grey, a novel mutation in the murine Lyst gene, causes the beige phenotype by skipping of exon 25.
Mammalian genome : official journal of the International Mammalian Genome Society17(3)203-10(2006 Mar)
Runkel F,B?ssow H,Seburn KL,Cox GA,Ward DM,Kaplan J,Franz T

Histological study of granulated metrial gland (GMG) cells in beige (DA-bg/bg) rats.
The Journal of reproduction and development51(6)799-803(2005 Dec)
Hondo E,Kokubu K,Nagahisa A,Nakamuta N,Ozaki K,Sagara E,Kiso Y

A new beige mutant rat ACI/N-Lystbg-Kyo.
Experimental animals52(1)31-6(2003 Jan)
Mori M,Yamasaki K,Nakanishi S,Kitada K,Higuchi K,Namiki C,Hamada S,Serikawa T

The rat lysosomal trafficking regulator (Lyst) gene is mapped on the telomeric region of chromosome 17.
Experimental animals52(1)89-91(2003 Jan)
Masui N,Nishikawa T,Takagi Y,Mori M,Suzuki T,Sato K

Slate--a new coat color mutant in the mouse.
The Journal of heredity5447-50(1963 Mar-Apr)
PIERRO LJ,CHASE HB

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Lyst cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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