Slc25a27 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Slc25a27
Entrez Gene ID	85262
Full Name	solute carrier family 25, member 27
Synonyms	Ucp4
General protein information	Preferred Names solute carrier family 25, member 27 Names mitochondrial uncoupling protein 4 uncoupling protein UCP-4
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 9 Map Location: 9q13
Summary	involved in mitochondrial proton leak [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_006244612.3	XP_006244674.1	mitochondrial uncoupling protein 4 isoform X3
XM_006244611.3	XP_006244673.1	mitochondrial uncoupling protein 4 isoform X2
XM_006244610.3	XP_006244672.1	mitochondrial uncoupling protein 4 isoform X1
XM_008766882.2	XP_008765104.1	mitochondrial uncoupling protein 4 isoform X4
NM_053500.1	NP_445952.1	mitochondrial uncoupling protein 4

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	SLC25A27	XP_852329.1
Mus musculus (house mouse)	Slc25a27	NP_082987.1
Homo sapiens (human)	SLC25A27	NP_004268.3
	SLC25A27	XP_001103050.1
Pan troglodytes (chimpanzee)	SLC25A27	XP_527398.1
Danio rerio (zebrafish)	slc25a27	NP_956635.1
Arabidopsis thaliana (thale cress)	AT1G14140	NP_172866.1
Rattus norvegicus (Norway rat)	Slc25a27	NP_445952.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009603	XP_318630.3
Caenorhabditis elegans (roundworm)	ucp-4	NP_505414.1
Xenopus tropicalis (tropical clawed frog)	slc25a27	NP_001011241.1
Bos taurus (cattle)	SLC25A27	NP_001193123.1
Drosophila melanogaster (fruit fly)	Ucp4A	NP_001188664.1

Gene Ontology
Bibliography

Related articles in PubMed

Effects of UCP4 on the Proliferation and Apoptosis of Chondrocytes: Its Possible Involvement and Regulation in Osteoarthritis.
Huang Z, Li J, Du S, Chen G, Qi Y, Huang L, Xiao L, Tong P
PloS one11(3)e0150684(2016)

Chronic alcohol consumption increases the expression of uncoupling protein-2 and -4 in the brain.
Graw JA, von Haefen C, Poyraz D, M?bius N, Sifringer M, Spies CD
Alcoholism, clinical and experimental research37(10)1650-6(2013 Oct)

Evidence for involvement of uncoupling proteins in cerebral mitochondrial oxidative phosphorylation deficiency of rats exposed to 5,000 m high altitude.
Xu Y, Liu Y, Xia C, Gao P, Liu JZ
Neurochemical research38(2)282-9(2013 Feb)

Role of mitochondrial uncoupling protein 4 in rat inner ear.
Smorodchenko A, Rupprecht A, Fuchs J, Gross J, Pohl EE
Molecular and cellular neurosciences47(4)244-53(2011 Aug)

UCP4 overexpression improves fatty acid oxidation and insulin sensitivity in L6 myocytes.
Gao CL, Ni YH, Liu G, Chen XH, Ji CB, Qin DN, Kou CZ, Zhu C, Zhang CM, Xia ZK, Guo XR
Journal of bioenergetics and biomembranes43(2)109-18(2011 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Silencing of UCP4 in primary chondrocytes significantly inhibited cell survival, but induced reactive oxygen species production and cell apoptosis.
Title: Effects of UCP4 on the Proliferation and Apoptosis of Chondrocytes: Its Possible Involvement and Regulation in Osteoarthritis.

A compensatory effect was found for UCP-4 (and UCP-2) in the brain during chronic alcohol consumption.
Title: Chronic alcohol consumption increases the expression of uncoupling protein-2 and -4 in the brain.

After high altitude exposure, UCP-4 mRNA abundance is 2.9-fold compared with the control; UCP-5 mRNA abundance is 2.3-fold compared with the control.
Title: Evidence for involvement of uncoupling proteins in cerebral mitochondrial oxidative phosphorylation deficiency of rats exposed to 5,000 m high altitude.

Data demonstrate that UCP4 expression is differently regulated during postnatal stages and is region-specific, and may play an important role in functional maturation of the rat inner ear.
Title: Role of mitochondrial uncoupling protein 4 in rat inner ear.

UCP4 overexpression reduced the insulin sensitivity and mitochondrial fatty acid oxidation of L6 myocytes.
Title: UCP4 overexpression improves fatty acid oxidation and insulin sensitivity in L6 myocytes.

The following Slc25a27 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Slc25a27 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa13501	NM_053500.1 Latest version!	Rattus norvegicus solute carrier family 25, member 27 (Slc25a27), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00
ORa32053	XM_006244610.3 Latest version!	Rattus norvegicus solute carrier family 25, member 27 (Slc25a27), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
ORa32054	XM_006244611.3 Latest version!	Rattus norvegicus solute carrier family 25, member 27 (Slc25a27), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
ORa32055	XM_006244612.3 Latest version!	Rattus norvegicus solute carrier family 25, member 27 (Slc25a27), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
ORa43866	XM_008766882.2 Latest version!	Rattus norvegicus solute carrier family 25, member 27 (Slc25a27), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Slc25a27 ( NM_053500.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_445952.1 Rattus norvegicus solute carrier family 25, member 27 (Slc25a27), mRNA.

Price & Availability↑

CloneID	ORa13501
Clone ID Related Accession (Same CDS sequence)	NM_053500.1
Accession Version	NM_053500.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 969bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-26
Organism	Rattus norvegicus(Norway rat)
Product	mitochondrial uncoupling protein 4
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AJ300162.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AJ300162.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGCCTTTCC CTGAGGAGGA GTCGCTACAG CCACTCACGC AGAGATGGCC CCGGACCAGC 
AAGTTCCTAC TGTCCGGCTG CGCCGCAACC GTGGCCGAGC TAGCAACCTT TCCCCTCGAT 
CTCACAAAAA CCCGACTCCA GATGCAAGGA GAAGCTGCCC TTGCTAAGTT GGGAGATGGT 
GCGATGGAAT CCGCCCCTTA CAGGGGCATG ATGCGCACGG CCCTGGGGAT TGTCCAGGAG 
GAAGGCTTCC TAAAGTTGTG GCAAGGAGTG ACGCCCGCCA TTTACAGACA CGTAGTGTAC 
TCTGGAGGTC GGATGGTCAC CTACGAACAT CTCCGGGAAG TTGTGTTTGG CAAAAGTGAA 
GATGAACATT ATCCCCTCTG GAAGTCCGTC ATTGGAGGGA TGATGGCTGG CGTCATCGGA 
CAGTTTTTAG CCAACCCCAC TGACCTCGTG AAGGTCCAGA TGCAAATGGA AGGGAAACGT 
AGACTGGAAG GGAAACCCTT GAGATTCCGT GGAGTACATC ATGCGTTTGC AAAAATCTTA 
GCTGAAGGAG GAATCCGTGG GCTTTGGGCA GGCTGGATAC CCAATATTCA GAGAGCAGCA 
CTTGTGAACA TGGGAGATTT AACCACTTAC GACACAGTGA AGCACTACCT GGTATTGAAC 
ACAGCACTTG AAGACAATAT CGCCACTCAC GGCTTATCCA GTTTGTGTTC TGGACTGGTG 
GCTTCTATTC TGGGAACACC AGCCGATGTC ATCAAAAGCC GAATAATGAA CCAACCTCGA 
GACAAACAAG GAAGAGGGCT TTTGTATAAG TCATCAACTG ACTGCGTGAT CCAGGCTGTT 
CAAGGAGAAG GCTTCCTGAG CCTGTATAAA GGCTTTCTAC CCTCCTGGCT GAGAATGACC 
CCTTGGTCAA TGGTGTTCTG GCTTACTTAT GAAAAAATCA GACAGCTGAG TGGAGTCAGT 
CCATTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_445952.1
CDS	52..1020
Translation	MPFPEEESLQPLTQRWPRTSKFLLSGCAATVAELATFPLDLTKTRLQMQGEAALAKLGDGAMESAPYRGMMRTALGIVQEEGFLKLWQGVTPAIYRHVVYSGGRMVTYEHLREVVFGKSEDEHYPLWKSVIGGMMAGVIGQFLANPTDLVKVQMQMEGKRRLEGKPLRFRGVHHAFAKILAEGGIRGLWAGWIPNIQRAALVNMGDLTTYDTVKHYLVLNTALEDNIATHGLSSLCSGLVASILGTPADVIKSRIMNQPRDKQGRGLLYKSSTDCVIQAVQGEGFLSLYKGFLPSWLRMTPWSMVFWLTYEKIRQLSGVSPF

Target ORF information:

RefSeq Version	NM_053500.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus solute carrier family 25, member 27 (Slc25a27), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_053500.1

ORF Insert Sequence:

ATGCCTTTCC CTGAGGAGGA GTCGCTACAG CCACTCACGC AGAGATGGCC CCGGACCAGC 
AAGTTCCTAC TGTCCGGCTG CGCCGCAACC GTGGCCGAGC TAGCAACCTT TCCCCTCGAT 
CTCACAAAAA CCCGACTCCA GATGCAAGGA GAAGCTGCCC TTGCTAAGTT GGGAGATGGT 
GCGATGGAAT CCGCCCCTTA CAGGGGCATG ATGCGCACGG CCCTGGGGAT TGTCCAGGAG 
GAAGGCTTCC TAAAGTTGTG GCAAGGAGTG ACGCCCGCCA TTTACAGACA CGTAGTGTAC 
TCTGGAGGTC GGATGGTCAC CTACGAACAT CTCCGGGAAG TTGTGTTTGG CAAAAGTGAA 
GATGAACATT ATCCCCTCTG GAAGTCCGTC ATTGGAGGGA TGATGGCTGG CGTCATCGGA 
CAGTTTTTAG CCAACCCCAC TGACCTCGTG AAGGTCCAGA TGCAAATGGA AGGGAAACGT 
AGACTGGAAG GGAAACCCTT GAGATTCCGT GGAGTACATC ATGCGTTTGC AAAAATCTTA 
GCTGAAGGAG GAATCCGTGG GCTTTGGGCA GGCTGGATAC CCAATATTCA GAGAGCAGCA 
CTTGTGAACA TGGGAGATTT AACCACTTAC GACACAGTGA AGCACTACCT GGTATTGAAC 
ACAGCACTTG AAGACAATAT CGCCACTCAC GGCTTATCCA GTTTGTGTTC TGGACTGGTG 
GCTTCTATTC TGGGAACACC AGCCGATGTC ATCAAAAGCC GAATAATGAA CCAACCTCGA 
GACAAACAAG GAAGAGGGCT TTTGTATAAG TCATCAACTG ACTGCGTGAT CCAGGCTGTT 
CAAGGAGAAG GCTTCCTGAG CCTGTATAAA GGCTTTCTAC CCTCCTGGCT GAGAATGACC 
CCTTGGTCAA TGGTGTTCTG GCTTACTTAT GAAAAAATCA GACAGCTGAG TGGAGTCAGT 
CCATTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Slc25a27 ( XM_006244610.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006244672.1 Rattus norvegicus solute carrier family 25, member 27 (Slc25a27), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa32053
Clone ID Related Accession (Same CDS sequence)	XM_006244610.3
Accession Version	XM_006244610.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1098bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	mitochondrial uncoupling protein 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005108.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006244610.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTTTCC CTGAGGAGGA GTCGCTACAG CCACTCACGC AGAGATGGCC CCGGACCAGC 
AAGTTCCTAC TGTCCGGCTG CGCCGCAACC GTGGCCGAGC TAGCAACCTT TCCCCTCGAT 
CTCACAAAAA CCCGACTCCA GATGCAAGGA GAAGCTGCCC TTGCTAAGCT GGGAGATGGT 
GCGATGGAAT CCGCCCCTTA CAGGGGCATG ATGCGCACGG CCCTGGGGAT TGTCCAGGAG 
GAAGGCTTCC TAAAGTTGTG GCAAGGAGTG ACGCCCGCCA TTTACAGACA CGTAGTGTAC 
TCTGGAGGTC GGATGGTCAC CTACGAACAT CTCCGGGAAG TTGTGTTTGG CAAAAGTGAA 
GATGAACATT ATCCCCTCTG GAAGTCCGTC ATTGGAGGGA TGATGGCTGG CGTCATCGGA 
CAGTTTTTAG CCAACCCCAC TGACCTCGTG AAGGTCCAGA TGCAAATGGA AGGGAAACGT 
AGACTGGAAG GGAAACCCTT GAGATTCCGG GGAGTACATC ACGCGTTTGC AAAAATCTTA 
GCTGAAGGAG GAATCCGTGG GCTTTGGGCA GGCTGGATAC CCAATATTCA GAGAGCAGCA 
CTTGTGAACA TGGGAGATTT AACCACTTAC GACACAGTGA AGCACTACCT GGTATTGAAC 
ACAGCACTTG AAGACAATAT CGCCACTCAC GGCTTATCCA GTTTGTGTTC TGGACTGGTG 
GCTTCTATTC TGGGAACACC AGCCGATGTC ATCAAAAGCC GAATAATGAA CCAACCTCGA 
GACAAACAAG GAAGAGGGCT TTTGTATAAG TCATCAACTG ACTGCGTGAT CCAGGCTGTT 
CAAGGAGAAG GCTTCCTGAG CCTGTATAAA GGCTTTCTAC CCTCCTGGCT GAGAATGGTA 
AAGACGGGGA GGTTCTGTTT CTTCCTTTGT TTTCTTTATA TTTCACTTAC TTGTACTTTG 
CAGGTATTTT TCATCCTCCT CTCTGATGTG TGGCCACACG AGAGGCCCAT CCACCTTCCT 
TTTAAAAATC TTTACTGTAG GGGTTTCTTT TTCTTTCAAC CCTTTCTTAG AATCATGTTT 
CCTTTCTTGA CAGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006244672.1
CDS	169..1266
Translation	MPFPEEESLQPLTQRWPRTSKFLLSGCAATVAELATFPLDLTKTRLQMQGEAALAKLGDGAMESAPYRGMMRTALGIVQEEGFLKLWQGVTPAIYRHVVYSGGRMVTYEHLREVVFGKSEDEHYPLWKSVIGGMMAGVIGQFLANPTDLVKVQMQMEGKRRLEGKPLRFRGVHHAFAKILAEGGIRGLWAGWIPNIQRAALVNMGDLTTYDTVKHYLVLNTALEDNIATHGLSSLCSGLVASILGTPADVIKSRIMNQPRDKQGRGLLYKSSTDCVIQAVQGEGFLSLYKGFLPSWLRMVKTGRFCFFLCFLYISLTCTLQVFFILLSDVWPHERPIHLPFKNLYCRGFFFFQPFLRIMFPFLTD

Target ORF information:

RefSeq Version	XM_006244610.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus solute carrier family 25, member 27 (Slc25a27), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006244610.3

ORF Insert Sequence:

ATGCCTTTCC CTGAGGAGGA GTCGCTACAG CCACTCACGC AGAGATGGCC CCGGACCAGC 
AAGTTCCTAC TGTCCGGCTG CGCCGCAACC GTGGCCGAGC TAGCAACCTT TCCCCTCGAT 
CTCACAAAAA CCCGACTCCA GATGCAAGGA GAAGCTGCCC TTGCTAAGCT GGGAGATGGT 
GCGATGGAAT CCGCCCCTTA CAGGGGCATG ATGCGCACGG CCCTGGGGAT TGTCCAGGAG 
GAAGGCTTCC TAAAGTTGTG GCAAGGAGTG ACGCCCGCCA TTTACAGACA CGTAGTGTAC 
TCTGGAGGTC GGATGGTCAC CTACGAACAT CTCCGGGAAG TTGTGTTTGG CAAAAGTGAA 
GATGAACATT ATCCCCTCTG GAAGTCCGTC ATTGGAGGGA TGATGGCTGG CGTCATCGGA 
CAGTTTTTAG CCAACCCCAC TGACCTCGTG AAGGTCCAGA TGCAAATGGA AGGGAAACGT 
AGACTGGAAG GGAAACCCTT GAGATTCCGG GGAGTACATC ACGCGTTTGC AAAAATCTTA 
GCTGAAGGAG GAATCCGTGG GCTTTGGGCA GGCTGGATAC CCAATATTCA GAGAGCAGCA 
CTTGTGAACA TGGGAGATTT AACCACTTAC GACACAGTGA AGCACTACCT GGTATTGAAC 
ACAGCACTTG AAGACAATAT CGCCACTCAC GGCTTATCCA GTTTGTGTTC TGGACTGGTG 
GCTTCTATTC TGGGAACACC AGCCGATGTC ATCAAAAGCC GAATAATGAA CCAACCTCGA 
GACAAACAAG GAAGAGGGCT TTTGTATAAG TCATCAACTG ACTGCGTGAT CCAGGCTGTT 
CAAGGAGAAG GCTTCCTGAG CCTGTATAAA GGCTTTCTAC CCTCCTGGCT GAGAATGGTA 
AAGACGGGGA GGTTCTGTTT CTTCCTTTGT TTTCTTTATA TTTCACTTAC TTGTACTTTG 
CAGGTATTTT TCATCCTCCT CTCTGATGTG TGGCCACACG AGAGGCCCAT CCACCTTCCT 
TTTAAAAATC TTTACTGTAG GGGTTTCTTT TTCTTTCAAC CCTTTCTTAG AATCATGTTT 
CCTTTCTTGA CAGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Slc25a27 ( XM_006244611.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006244673.1 Rattus norvegicus solute carrier family 25, member 27 (Slc25a27), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa32054
Clone ID Related Accession (Same CDS sequence)	XM_006244611.3
Accession Version	XM_006244611.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1035bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	mitochondrial uncoupling protein 4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005108.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006244611.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTTTCC CTGAGGAGGA GTCGCTACAG CCACTCACGC AGAGATGGCC CCGGACCAGC 
AAGTTCCTAC TGTCCGGCTG CGCCGCAACC GTGGCCGAGC TAGCAACCTT TCCCCTCGAT 
CTCACAAAAA CCCGACTCCA GATGCAAGGA GAAGCTGCCC TTGCTAAGCT GGGAGATGGT 
GCGATGGAAT CCGCCCCTTA CAGGGGCATG ATGCGCACGG CCCTGGGGAT TGTCCAGGAG 
GAAGGCTTCC TAAAGTTGTG GCAAGGAGTG ACGCCCGCCA TTTACAGACA CGTAGTGTAC 
TCTGGAGGTC GGATGGTCAC CTACGAACAT CTCCGGGAAG TTGTGTTTGG CAAAAGTGAA 
GATGAACATT ATCCCCTCTG GAAGTCCGTC ATTGGAGGGA TGATGGCTGG CGTCATCGGA 
CAGTTTTTAG CCAACCCCAC TGACCTCGTG AAGGTCCAGA TGCAAATGGA AGGGAAACGT 
AGACTGGAAG GGAAACCCTT GAGATTCCGG GGAGTACATC ACGCGTTTGC AAAAATCTTA 
GCTGAAGGAG GAATCCGTGG GCTTTGGGCA GGCTGGATAC CCAATATTCA GAGAGCAGCA 
CTTGTGAACA TGGGAGATTT AACCACTTAC GACACAGTGA AGCACTACCT GGTATTGAAC 
ACAGCACTTG AAGACAATAT CGCCACTCAC GGCTTATCCA GTTTGTGTTC TGGACTGGTG 
GCTTCTATTC TGGGAACACC AGCCGATGTC ATCAAAAGCC GAATAATGAA CCAACCTCGA 
GACAAACAAG GAAGAGGGCT TTTGTATAAG TCATCAACTG ACTGCGTGAT CCAGGCTGTT 
CAAGGAGAAG GCTTCCTGAG CCTGTATAAA GGCTTTCTAC CCTCCTGGCT GAGAATGGAA 
GATACTTTAC TCCACAGAGA CTGCGATTTG CAGGGGGCAA CACTTTATCC CTCTCTGGAA 
ACCGGAGTTC TCTCTGACTC CTCCTTTTAT CTAAGAGCAA TCTGTCTGGT CCAGTCTCAC 
GAGACCAACC ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006244673.1
CDS	169..1203
Translation	MPFPEEESLQPLTQRWPRTSKFLLSGCAATVAELATFPLDLTKTRLQMQGEAALAKLGDGAMESAPYRGMMRTALGIVQEEGFLKLWQGVTPAIYRHVVYSGGRMVTYEHLREVVFGKSEDEHYPLWKSVIGGMMAGVIGQFLANPTDLVKVQMQMEGKRRLEGKPLRFRGVHHAFAKILAEGGIRGLWAGWIPNIQRAALVNMGDLTTYDTVKHYLVLNTALEDNIATHGLSSLCSGLVASILGTPADVIKSRIMNQPRDKQGRGLLYKSSTDCVIQAVQGEGFLSLYKGFLPSWLRMEDTLLHRDCDLQGATLYPSLETGVLSDSSFYLRAICLVQSHETNH

Target ORF information:

RefSeq Version	XM_006244611.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus solute carrier family 25, member 27 (Slc25a27), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006244611.3

ORF Insert Sequence:

ATGCCTTTCC CTGAGGAGGA GTCGCTACAG CCACTCACGC AGAGATGGCC CCGGACCAGC 
AAGTTCCTAC TGTCCGGCTG CGCCGCAACC GTGGCCGAGC TAGCAACCTT TCCCCTCGAT 
CTCACAAAAA CCCGACTCCA GATGCAAGGA GAAGCTGCCC TTGCTAAGCT GGGAGATGGT 
GCGATGGAAT CCGCCCCTTA CAGGGGCATG ATGCGCACGG CCCTGGGGAT TGTCCAGGAG 
GAAGGCTTCC TAAAGTTGTG GCAAGGAGTG ACGCCCGCCA TTTACAGACA CGTAGTGTAC 
TCTGGAGGTC GGATGGTCAC CTACGAACAT CTCCGGGAAG TTGTGTTTGG CAAAAGTGAA 
GATGAACATT ATCCCCTCTG GAAGTCCGTC ATTGGAGGGA TGATGGCTGG CGTCATCGGA 
CAGTTTTTAG CCAACCCCAC TGACCTCGTG AAGGTCCAGA TGCAAATGGA AGGGAAACGT 
AGACTGGAAG GGAAACCCTT GAGATTCCGG GGAGTACATC ACGCGTTTGC AAAAATCTTA 
GCTGAAGGAG GAATCCGTGG GCTTTGGGCA GGCTGGATAC CCAATATTCA GAGAGCAGCA 
CTTGTGAACA TGGGAGATTT AACCACTTAC GACACAGTGA AGCACTACCT GGTATTGAAC 
ACAGCACTTG AAGACAATAT CGCCACTCAC GGCTTATCCA GTTTGTGTTC TGGACTGGTG 
GCTTCTATTC TGGGAACACC AGCCGATGTC ATCAAAAGCC GAATAATGAA CCAACCTCGA 
GACAAACAAG GAAGAGGGCT TTTGTATAAG TCATCAACTG ACTGCGTGAT CCAGGCTGTT 
CAAGGAGAAG GCTTCCTGAG CCTGTATAAA GGCTTTCTAC CCTCCTGGCT GAGAATGGAA 
GATACTTTAC TCCACAGAGA CTGCGATTTG CAGGGGGCAA CACTTTATCC CTCTCTGGAA 
ACCGGAGTTC TCTCTGACTC CTCCTTTTAT CTAAGAGCAA TCTGTCTGGT CCAGTCTCAC 
GAGACCAACC ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Slc25a27 ( XM_006244612.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006244674.1 Rattus norvegicus solute carrier family 25, member 27 (Slc25a27), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa32055
Clone ID Related Accession (Same CDS sequence)	XM_006244612.3
Accession Version	XM_006244612.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 957bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	mitochondrial uncoupling protein 4 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005108.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006244612.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTTTCC CTGAGGAGGA GTCGCTACAG CCACTCACGC AGAGATGGCC CCGGACCAGC 
AAGTTCCTAC TGTCCGGCTG CGCCGCAACC GTGGCCGAGC TAGCAACCTT TCCCCTCGAT 
CTCACAAAAA CCCGACTCCA GATGCAAGGA GAAGCTGCCC TTGCTAAGCT GGGAGATGGT 
GCGATGGAAT CCGCCCCTTA CAGGGGCATG ATGCGCACGG CCCTGGGGAT TGTCCAGGAG 
GAAGGCTTCC TAAAGTTGTG GCAAGGAGTG ACGCCCGCCA TTTACAGACA CGTAGTGTAC 
TCTGGAGGTC GGATGGTCAC CTACGAACAT CTCCGGGAAG TTGTGTTTGG CAAAAGTGAA 
GATGAACATT ATCCCCTCTG GAAGTCCGTC ATTGGAGGGA TGATGGCTGG CGTCATCGGA 
CAGTTTTTAG CCAACCCCAC TGACCTCGTG AAGGTCCAGA TGCAAATGGA AGGGAAACGT 
AGACTGGAAG GGAAACCCTT GAGATTCCGG GGAGTACATC ACGCGTTTGC AAAAATCTTA 
GCTGAAGGAG GAATCCGTGG GCTTTGGGCA GGCTGGATAC CCAATATTCA GAGAGCAGCA 
CTTGTGAACA TGGGAGATTT AACCACTTAC GACACAGTGA AGCACTACCT GGTATTGAAC 
ACAGCACTTG AAGACAATAT CGCCACTCAC GGCTTATCCA GTTTGTGTTC TGGACTGGTG 
GCTTCTATTC TGGGAACACC AGCCGATGTC ATCAAAAGCC GAATAATGAA CCAACCTCGA 
GACAAACAAG GAAGAGGGCT TTTGTATAAG TCATCAACTG ACTGCGTGAT CCAGGCTGTT 
CAAGGAGAAG GCTTCCTGAG CCTGTATAAA GGCTTTCTAC CCTCCTGGCT GAGAATGAGA 
CTGCGATTTG CAGGGGGCAA CACTTTATCC CTCTCTGGAA ACCGGAGTTC TCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006244674.1
CDS	169..1125
Translation	MPFPEEESLQPLTQRWPRTSKFLLSGCAATVAELATFPLDLTKTRLQMQGEAALAKLGDGAMESAPYRGMMRTALGIVQEEGFLKLWQGVTPAIYRHVVYSGGRMVTYEHLREVVFGKSEDEHYPLWKSVIGGMMAGVIGQFLANPTDLVKVQMQMEGKRRLEGKPLRFRGVHHAFAKILAEGGIRGLWAGWIPNIQRAALVNMGDLTTYDTVKHYLVLNTALEDNIATHGLSSLCSGLVASILGTPADVIKSRIMNQPRDKQGRGLLYKSSTDCVIQAVQGEGFLSLYKGFLPSWLRMRLRFAGGNTLSLSGNRSSL

Target ORF information:

RefSeq Version	XM_006244612.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus solute carrier family 25, member 27 (Slc25a27), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006244612.3

ORF Insert Sequence:

ATGCCTTTCC CTGAGGAGGA GTCGCTACAG CCACTCACGC AGAGATGGCC CCGGACCAGC 
AAGTTCCTAC TGTCCGGCTG CGCCGCAACC GTGGCCGAGC TAGCAACCTT TCCCCTCGAT 
CTCACAAAAA CCCGACTCCA GATGCAAGGA GAAGCTGCCC TTGCTAAGCT GGGAGATGGT 
GCGATGGAAT CCGCCCCTTA CAGGGGCATG ATGCGCACGG CCCTGGGGAT TGTCCAGGAG 
GAAGGCTTCC TAAAGTTGTG GCAAGGAGTG ACGCCCGCCA TTTACAGACA CGTAGTGTAC 
TCTGGAGGTC GGATGGTCAC CTACGAACAT CTCCGGGAAG TTGTGTTTGG CAAAAGTGAA 
GATGAACATT ATCCCCTCTG GAAGTCCGTC ATTGGAGGGA TGATGGCTGG CGTCATCGGA 
CAGTTTTTAG CCAACCCCAC TGACCTCGTG AAGGTCCAGA TGCAAATGGA AGGGAAACGT 
AGACTGGAAG GGAAACCCTT GAGATTCCGG GGAGTACATC ACGCGTTTGC AAAAATCTTA 
GCTGAAGGAG GAATCCGTGG GCTTTGGGCA GGCTGGATAC CCAATATTCA GAGAGCAGCA 
CTTGTGAACA TGGGAGATTT AACCACTTAC GACACAGTGA AGCACTACCT GGTATTGAAC 
ACAGCACTTG AAGACAATAT CGCCACTCAC GGCTTATCCA GTTTGTGTTC TGGACTGGTG 
GCTTCTATTC TGGGAACACC AGCCGATGTC ATCAAAAGCC GAATAATGAA CCAACCTCGA 
GACAAACAAG GAAGAGGGCT TTTGTATAAG TCATCAACTG ACTGCGTGAT CCAGGCTGTT 
CAAGGAGAAG GCTTCCTGAG CCTGTATAAA GGCTTTCTAC CCTCCTGGCT GAGAATGAGA 
CTGCGATTTG CAGGGGGCAA CACTTTATCC CTCTCTGGAA ACCGGAGTTC TCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Slc25a27 ( XM_008766882.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008765104.1 Rattus norvegicus solute carrier family 25, member 27 (Slc25a27), transcript variant X4, mRNA.

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CloneID	ORa43866
Clone ID Related Accession (Same CDS sequence)	XM_008766882.2
Accession Version	XM_008766882.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 948bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	mitochondrial uncoupling protein 4 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005108.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008766882.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGGAG CAGATGCAAC CTTTCCCCTC GATCTCACAA AAACCCGACT CCAGATGCAA 
GGAGAAGCTG CCCTTGCTAA GCTGGGAGAT GGTGCGATGG AATCCGCCCC TTACAGGGGC 
ATGATGCGCA CGGCCCTGGG GATTGTCCAG GAGGAAGGCT TCCTAAAGTT GTGGCAAGGA 
GTGACGCCCG CCATTTACAG ACACGTAGTG TACTCTGGAG GTCGGATGGT CACCTACGAA 
CATCTCCGGG AAGTTGTGTT TGGCAAAAGT GAAGATGAAC ATTATCCCCT CTGGAAGTCC 
GTCATTGGAG GGATGATGGC TGGCGTCATC GGACAGTTTT TAGCCAACCC CACTGACCTC 
GTGAAGGTCC AGATGCAAAT GGAAGGGAAA CGTAGACTGG AAGGGAAACC CTTGAGATTC 
CGGGGAGTAC ATCACGCGTT TGCAAAAATC TTAGCTGAAG GAGGAATCCG TGGGCTTTGG 
GCAGGCTGGA TACCCAATAT TCAGAGAGCA GCACTTGTGA ACATGGGAGA TTTAACCACT 
TACGACACAG TGAAGCACTA CCTGGTATTG AACACAGCAC TTGAAGACAA TATCGCCACT 
CACGGCTTAT CCAGTTTGTG TTCTGGACTG GTGGCTTCTA TTCTGGGAAC ACCAGCCGAT 
GTCATCAAAA GCCGAATAAT GAACCAACCT CGAGACAAAC AAGGAAGAGG GCTTTTGTAT 
AAGTCATCAA CTGACTGCGT GATCCAGGCT GTTCAAGGAG AAGGCTTCCT GAGCCTGTAT 
AAAGGCTTTC TACCCTCCTG GCTGAGAATG GAAGATACTT TACTCCACAG AGACTGCGAT 
TTGCAGGGGG CAACACTTTA TCCCTCTCTG GAAACCGGAG TTCTCTCTGA CTCCTCCTTT 
TATCTAAGAG CAATCTGTCT GGTCCAGTCT CACGAGACCA ACCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008765104.1
CDS	477..1424
Translation	MAGADATFPLDLTKTRLQMQGEAALAKLGDGAMESAPYRGMMRTALGIVQEEGFLKLWQGVTPAIYRHVVYSGGRMVTYEHLREVVFGKSEDEHYPLWKSVIGGMMAGVIGQFLANPTDLVKVQMQMEGKRRLEGKPLRFRGVHHAFAKILAEGGIRGLWAGWIPNIQRAALVNMGDLTTYDTVKHYLVLNTALEDNIATHGLSSLCSGLVASILGTPADVIKSRIMNQPRDKQGRGLLYKSSTDCVIQAVQGEGFLSLYKGFLPSWLRMEDTLLHRDCDLQGATLYPSLETGVLSDSSFYLRAICLVQSHETNH

Target ORF information:

RefSeq Version	XM_008766882.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus solute carrier family 25, member 27 (Slc25a27), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008766882.2

ORF Insert Sequence:

ATGGCTGGAG CAGATGCAAC CTTTCCCCTC GATCTCACAA AAACCCGACT CCAGATGCAA 
GGAGAAGCTG CCCTTGCTAA GCTGGGAGAT GGTGCGATGG AATCCGCCCC TTACAGGGGC 
ATGATGCGCA CGGCCCTGGG GATTGTCCAG GAGGAAGGCT TCCTAAAGTT GTGGCAAGGA 
GTGACGCCCG CCATTTACAG ACACGTAGTG TACTCTGGAG GTCGGATGGT CACCTACGAA 
CATCTCCGGG AAGTTGTGTT TGGCAAAAGT GAAGATGAAC ATTATCCCCT CTGGAAGTCC 
GTCATTGGAG GGATGATGGC TGGCGTCATC GGACAGTTTT TAGCCAACCC CACTGACCTC 
GTGAAGGTCC AGATGCAAAT GGAAGGGAAA CGTAGACTGG AAGGGAAACC CTTGAGATTC 
CGGGGAGTAC ATCACGCGTT TGCAAAAATC TTAGCTGAAG GAGGAATCCG TGGGCTTTGG 
GCAGGCTGGA TACCCAATAT TCAGAGAGCA GCACTTGTGA ACATGGGAGA TTTAACCACT 
TACGACACAG TGAAGCACTA CCTGGTATTG AACACAGCAC TTGAAGACAA TATCGCCACT 
CACGGCTTAT CCAGTTTGTG TTCTGGACTG GTGGCTTCTA TTCTGGGAAC ACCAGCCGAT 
GTCATCAAAA GCCGAATAAT GAACCAACCT CGAGACAAAC AAGGAAGAGG GCTTTTGTAT 
AAGTCATCAA CTGACTGCGT GATCCAGGCT GTTCAAGGAG AAGGCTTCCT GAGCCTGTAT 
AAAGGCTTTC TACCCTCCTG GCTGAGAATG GAAGATACTT TACTCCACAG AGACTGCGAT 
TTGCAGGGGG CAACACTTTA TCCCTCTCTG GAAACCGGAG TTCTCTCTGA CTCCTCCTTT 
TATCTAAGAG CAATCTGTCT GGTCCAGTCT CACGAGACCA ACCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Effects of UCP4 on the Proliferation and Apoptosis of Chondrocytes: Its Possible Involvement and Regulation in Osteoarthritis.
PloS one11(3)e0150684(2016)
Huang Z,Li J,Du S,Chen G,Qi Y,Huang L,Xiao L,Tong P

Chronic alcohol consumption increases the expression of uncoupling protein-2 and -4 in the brain.
Alcoholism, clinical and experimental research37(10)1650-6(2013 Oct)
Graw JA,von Haefen C,Poyraz D,M?bius N,Sifringer M,Spies CD

Evidence for involvement of uncoupling proteins in cerebral mitochondrial oxidative phosphorylation deficiency of rats exposed to 5,000 m high altitude.
Neurochemical research38(2)282-9(2013 Feb)
Xu Y,Liu Y,Xia C,Gao P,Liu JZ

Role of mitochondrial uncoupling protein 4 in rat inner ear.
Molecular and cellular neurosciences47(4)244-53(2011 Aug)
Smorodchenko A,Rupprecht A,Fuchs J,Gross J,Pohl EE

UCP4 overexpression improves fatty acid oxidation and insulin sensitivity in L6 myocytes.
Journal of bioenergetics and biomembranes43(2)109-18(2011 Apr)
Gao CL,Ni YH,Liu G,Chen XH,Ji CB,Qin DN,Kou CZ,Zhu C,Zhang CM,Xia ZK,Guo XR

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Slc25a27 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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