Cnga3 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cnga3
Entrez Gene ID	85257
Full Name	cyclic nucleotide gated channel alpha 3
General protein information	Preferred Names cyclic nucleotide gated channel alpha 3 Names cyclic nucleotide-gated cation channel alpha-3
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 9 Map Location: 9q21
Summary	a subunit of a cGMP-selective cyclic nucleotide-gated (CNG) channel; may have a role in olfaction and vision [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_017596680.1	XP_017452169.1	cyclic nucleotide-gated cation channel alpha-3 isoform X1
XM_008767043.2	XP_008765265.1	cyclic nucleotide-gated cation channel alpha-3 isoform X3
XM_008767042.2	XP_008765264.1	cyclic nucleotide-gated cation channel alpha-3 isoform X1
XM_017596681.1	XP_017452170.1	cyclic nucleotide-gated cation channel alpha-3 isoform X2
NM_053495.1	NP_445947.1	cyclic nucleotide-gated cation channel alpha-3

Pathways from BioSystems

Homologies

Caenorhabditis elegans (roundworm)	tax-4	NP_499033.1
Homo sapiens (human)	CNGA3	NP_001289.1
Mus musculus (house mouse)	Cnga3	NP_001268939.1
Xenopus tropicalis (tropical clawed frog)	LOC100494840	XP_002931690.2
	CNGA3	XP_001101944.2
Gallus gallus (chicken)	CNGA3	NP_990552.1
Canis lupus familiaris (dog)	CNGA3	XP_538462.3
Rattus norvegicus (Norway rat)	Cnga3	NP_445947.1
Danio rerio (zebrafish)	cnga3a	XP_005166141.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009050	XP_319802.4
Pan troglodytes (chimpanzee)	CNGA3	XP_001156943.1
Bos taurus (cattle)	CNGA3	NP_776704.1
Drosophila melanogaster (fruit fly)	Cng	NP_477116.1

Gene Ontology
Bibliography

Related articles in PubMed

Cyclic nucleotide-gated channel subunit glycosylation regulates matrix metalloproteinase-dependent changes in channel gating.
Meighan SE, Meighan PC, Rich ED, Brown RL, Varnum MD
Biochemistry52(46)8352-62(2013 Nov)

Cyclic nucleotide-gated channel ?-3 (CNGA3) interacts with stereocilia tip-link cadherin 23 + exon 68 or alternatively with myosin VIIa, two proteins required for hair cell mechanotransduction.
Selvakumar D, Drescher MJ, Drescher DG
The Journal of biological chemistry288(10)7215-29(2013 Mar)

Calcium signalling remodelling and disease.
Berridge MJ
Biochemical Society transactions40(2)297-309(2012 Apr)

CNGA3 is expressed in inner ear hair cells and binds to an intracellular C-terminus domain of EMILIN1.
Selvakumar D, Drescher MJ, Dowdall JR, Khan KM, Hatfield JS, Ramakrishnan NA, Drescher DG
The Biochemical journal443(2)463-76(2012 Apr)

Mouse model resources for vision research.
Won J, Shi LY, Hicks W, Wang J, Hurd R, Naggert JK, Chang B, Nishina PM
Journal of ophthalmology2011391384(2011)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data indicate that CNGA3 interacts with cadherin 23 and with cadherin 23 binding partner, myosin VIIa.
Title: Cyclic nucleotide-gated channel ?-3 (CNGA3) interacts with stereocilia tip-link cadherin 23 + exon 68 or alternatively with myosin VIIa, two proteins required for hair cell mechanotransduction.

Pull-down assays indicated that the binding to organ of Corti CNGA3 was attributable to the EMILIN1 intracellular sequence that follows a predicted transmembrane domain in the C-terminus
Title: CNGA3 is expressed in inner ear hair cells and binds to an intracellular C-terminus domain of EMILIN1.

This study showed that widespread expression of the CNGA3 in the rat olfactory bulb. appears in principal cells, including mitral cells and internal, medium and external tufted cells.
Title: Distribution of the A3 subunit of the cyclic nucleotide-gated ion channels in the main olfactory bulb of the rat.

Renal CNG-A3 mRNA and protein expression were lowered by high Na+ diet; aldosterone modulation was present in the cortical thick ascending loop and cortical collecting duct. Results suggests CNG-A3 may have a physiological role in sodium reabsorption.
Title: Modulation of renal CNG-A3 sodium channel in rats subjected to low- and high-sodium diets.

The following Cnga3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cnga3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa42249	NM_053495.1 Latest version!	Rattus norvegicus cyclic nucleotide gated channel subunit alpha 3 (Cnga3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
ORa43864	XM_008767043.2 Latest version!	Rattus norvegicus cyclic nucleotide gated channel alpha 3 (Cnga3), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
ORa43863	XM_017596680.1 Latest version!	Rattus norvegicus cyclic nucleotide gated channel alpha 3 (Cnga3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
ORa43863	XM_008767042.2 Latest version!	Rattus norvegicus cyclic nucleotide gated channel alpha 3 (Cnga3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
ORa62897	XM_017596681.1 Latest version!	Rattus norvegicus cyclic nucleotide gated channel alpha 3 (Cnga3), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cnga3 ( NM_053495.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_445947.1 Rattus norvegicus cyclic nucleotide gated channel subunit alpha 3 (Cnga3), mRNA.

Price & Availability↑

CloneID	ORa42249
Clone ID Related Accession (Same CDS sequence)	NM_053495.1
Accession Version	NM_053495.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1836bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-09
Organism	Rattus norvegicus(Norway rat)
Product	cyclic nucleotide-gated cation channel alpha-3
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AB002801.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB002801.1 [ECO:0000332] ##Evidence-Data-END##

ATGCATCAGA TGGAAACATC GACCATGGTC CAAGACAACA GGGTGTCACG CTTCATCATC 
TCCATTCGTG CGTGGGCCGC CAGGCACTTA CACCATGAAG ACCCGACGCC TGACTCCTTT 
TTGGATCGTT TTCATGGAGC TGAGCCTAAG GAAGTATCCA GCCAGGAAAG GAATGCCCAG 
CCCAACCCAG GAGGACAGGA ACCACCAGAG GGAGGGAAAG GCAGGAAGAA GGATCCCATT 
GTGGTGGACC CCTCCAGCAA CATCTACTAC CGCTGGCTGA CTGCCATCGC CCTCCCGGTC 
TTCTATAACT GGTGTCTACT TGTGTGCAGG GCCTGTTTTG ATGAGCTACA GTCAGAACAC 
CTGACACTGT GGCTGGTCCT GGACTACTCT GCAGATGCCC TGTATGTTGT GGACATGCTG 
GTTCGTGCCC GGACAGGTTT CCTTGAACAA GGCCTAATGG TCAGGGATAC CAAGAGGCTG 
TGGAAACATT ACACAAAGAC CTTGCACTTC AAGCTGGACA TCCTGTCTCT CATCCCCACA 
GACCTGGCTT ATTTGAAGTT GGGCATGAAC TACCCGGAAC TGAGGTTCAA TCGCCTCCTG 
AGGTTCTCTC GGCTCTTTGA GTTCTTTGAC CGCACGGAGA CAAGGACCAA CTACCCCAAT 
GTGTTCAGGA TAGGGAACTT GGTTCTGTAC ACCCTCATTA TCATCCACTG GAACGCCTGC 
ATCTACTTTG CCATTTCCAA GTTCATCGGT TTTGGAACAG ATTCCTGGGT CTATCCAAAC 
ACCTCCAAGC CGGAGTATGG ACGCCTCTCC AGGAAGTACA TTTACAGCCT CTACTGGTCC 
ACCTTGACCC TGACCACCAT TGGGGAGACC CCGCCCCCCG TGAAGGATGA GGAGTATCTC 
TTTGTGGTCA TAGACTTCCT GGTGGGCGTC CTGATCTTCG CCACTATAGT AGGCAATGTG 
GGCTCCATGA TTTCCAACAT GAACGCTTCC CGGGCGGAGT TCCAGGCTAA GATAGATTCC 
ATCAAGCAGT ACATGCAGTT CCGGAAGGTA ACCAAGGACT TGGAGACTCG GGTTATCCGG 
TGGTTTGACT ATCTGTGGGC TAACAGGAAG ACGGTGGATG AAAAGGAAGT GCTCAAAAAC 
CTCCCAGACA AGCTGAAGGC CGAGATCGCC ATCAACGTGC ACCTGGACAC ACTGAAGAAA 
GTCCGAATCT TCCAGGACTG CGAGGCAGGG CTTCTGGTGG AGCTGGTGCT GAAGCTTCGT 
CCTGCTGTGT TCAGCCCTGG GGACTACATT TGCAAAAAGG GGGACATTGG AAGGGAGATG 
TACATCATCA AGGAGGGCAA GCTGGCTGTC GTGGCTGACG ATGGGGTCAC CCAGTTTGTG 
GTCCTCAGTG ATGGCAGTTA CTTTGGGGAG ATCAGCATCT TAAACATTAA GGGGAGCAAG 
TCGGGGAACC GCAGGACAGC CAACATCAGG AGCATCGGCT ACTCGGACCT GTTCTGCCTC 
TCCAAGGATG ACTTGATGGA GACCCTCACG GAGTACCCGG ATGCTAAGAG GGCTCTGGAG 
GAAAAGGGTC GGCAGATTCT GATGAAGGAC AACCTAATCG ATGACGACCT GGTGACGGCC 
AGGGCAGATG CCAGGAACAT CGAGGAGAAG GTGGAGTACC TGGAGTCATC CTTGGACGGC 
CTGCAGACGA GGTTCGCCCG ACTCCTGGCT GAGTACAGTG CCTCCCAGAT GAAGCTGAAA 
CAGCGGCTCA GCCAGCTGGA GAGCCAGATG ACCAGGAGGG GTCATGGCTT CTCACCTGAC 
AGGGAGAATT CCGAGGATGC TTCAAAAGCT GACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_445947.1
CDS	14..1849
Translation	MHQMETSTMVQDNRVSRFIISIRAWAARHLHHEDPTPDSFLDRFHGAEPKEVSSQERNAQPNPGGQEPPEGGKGRKKDPIVVDPSSNIYYRWLTAIALPVFYNWCLLVCRACFDELQSEHLTLWLVLDYSADALYVVDMLVRARTGFLEQGLMVRDTKRLWKHYTKTLHFKLDILSLIPTDLAYLKLGMNYPELRFNRLLRFSRLFEFFDRTETRTNYPNVFRIGNLVLYTLIIIHWNACIYFAISKFIGFGTDSWVYPNTSKPEYGRLSRKYIYSLYWSTLTLTTIGETPPPVKDEEYLFVVIDFLVGVLIFATIVGNVGSMISNMNASRAEFQAKIDSIKQYMQFRKVTKDLETRVIRWFDYLWANRKTVDEKEVLKNLPDKLKAEIAINVHLDTLKKVRIFQDCEAGLLVELVLKLRPAVFSPGDYICKKGDIGREMYIIKEGKLAVVADDGVTQFVVLSDGSYFGEISILNIKGSKSGNRRTANIRSIGYSDLFCLSKDDLMETLTEYPDAKRALEEKGRQILMKDNLIDDDLVTARADARNIEEKVEYLESSLDGLQTRFARLLAEYSASQMKLKQRLSQLESQMTRRGHGFSPDRENSEDASKAD

Target ORF information:

RefSeq Version	NM_053495.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus cyclic nucleotide gated channel subunit alpha 3 (Cnga3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_053495.1

ORF Insert Sequence:

ATGCATCAGA TGGAAACATC GACCATGGTC CAAGACAACA GGGTGTCACG CTTCATCATC 
TCCATTCGTG CGTGGGCCGC CAGGCACTTA CACCATGAAG ACCCGACGCC TGACTCCTTT 
TTGGATCGTT TTCATGGAGC TGAGCCTAAG GAAGTATCCA GCCAGGAAAG GAATGCCCAG 
CCCAACCCAG GAGGACAGGA ACCACCAGAG GGAGGGAAAG GCAGGAAGAA GGATCCCATT 
GTGGTGGACC CCTCCAGCAA CATCTACTAC CGCTGGCTGA CTGCCATCGC CCTCCCGGTC 
TTCTATAACT GGTGTCTACT TGTGTGCAGG GCCTGTTTTG ATGAGCTACA GTCAGAACAC 
CTGACACTGT GGCTGGTCCT GGACTACTCT GCAGATGCCC TGTATGTTGT GGACATGCTG 
GTTCGTGCCC GGACAGGTTT CCTTGAACAA GGCCTAATGG TCAGGGATAC CAAGAGGCTG 
TGGAAACATT ACACAAAGAC CTTGCACTTC AAGCTGGACA TCCTGTCTCT CATCCCCACA 
GACCTGGCTT ATTTGAAGTT GGGCATGAAC TACCCGGAAC TGAGGTTCAA TCGCCTCCTG 
AGGTTCTCTC GGCTCTTTGA GTTCTTTGAC CGCACGGAGA CAAGGACCAA CTACCCCAAT 
GTGTTCAGGA TAGGGAACTT GGTTCTGTAC ACCCTCATTA TCATCCACTG GAACGCCTGC 
ATCTACTTTG CCATTTCCAA GTTCATCGGT TTTGGAACAG ATTCCTGGGT CTATCCAAAC 
ACCTCCAAGC CGGAGTATGG ACGCCTCTCC AGGAAGTACA TTTACAGCCT CTACTGGTCC 
ACCTTGACCC TGACCACCAT TGGGGAGACC CCGCCCCCCG TGAAGGATGA GGAGTATCTC 
TTTGTGGTCA TAGACTTCCT GGTGGGCGTC CTGATCTTCG CCACTATAGT AGGCAATGTG 
GGCTCCATGA TTTCCAACAT GAACGCTTCC CGGGCGGAGT TCCAGGCTAA GATAGATTCC 
ATCAAGCAGT ACATGCAGTT CCGGAAGGTA ACCAAGGACT TGGAGACTCG GGTTATCCGG 
TGGTTTGACT ATCTGTGGGC TAACAGGAAG ACGGTGGATG AAAAGGAAGT GCTCAAAAAC 
CTCCCAGACA AGCTGAAGGC CGAGATCGCC ATCAACGTGC ACCTGGACAC ACTGAAGAAA 
GTCCGAATCT TCCAGGACTG CGAGGCAGGG CTTCTGGTGG AGCTGGTGCT GAAGCTTCGT 
CCTGCTGTGT TCAGCCCTGG GGACTACATT TGCAAAAAGG GGGACATTGG AAGGGAGATG 
TACATCATCA AGGAGGGCAA GCTGGCTGTC GTGGCTGACG ATGGGGTCAC CCAGTTTGTG 
GTCCTCAGTG ATGGCAGTTA CTTTGGGGAG ATCAGCATCT TAAACATTAA GGGGAGCAAG 
TCGGGGAACC GCAGGACAGC CAACATCAGG AGCATCGGCT ACTCGGACCT GTTCTGCCTC 
TCCAAGGATG ACTTGATGGA GACCCTCACG GAGTACCCGG ATGCTAAGAG GGCTCTGGAG 
GAAAAGGGTC GGCAGATTCT GATGAAGGAC AACCTAATCG ATGACGACCT GGTGACGGCC 
AGGGCAGATG CCAGGAACAT CGAGGAGAAG GTGGAGTACC TGGAGTCATC CTTGGACGGC 
CTGCAGACGA GGTTCGCCCG ACTCCTGGCT GAGTACAGTG CCTCCCAGAT GAAGCTGAAA 
CAGCGGCTCA GCCAGCTGGA GAGCCAGATG ACCAGGAGGG GTCATGGCTT CTCACCTGAC 
AGGGAGAATT CCGAGGATGC TTCAAAAGCT GACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cnga3 ( XM_008767043.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008765265.1 Rattus norvegicus cyclic nucleotide gated channel alpha 3 (Cnga3), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ORa43864
Clone ID Related Accession (Same CDS sequence)	XM_008767043.2
Accession Version	XM_008767043.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1899bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	cyclic nucleotide-gated cation channel alpha-3 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005108.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008767043.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAGG TCAATACCCA ATGCTCACAG CCCTCCCCGA GACAACTTTC AGTAAAGGAC 
GCCGCAGACA GAGACCTCGA TCAAGTGGAG AATGGCCTCG GCAGGGTGTC ACGCTTCATC 
ATCTCCATTC GTGCGTGGGC CGCCAGGCAC TTACACCATG AAGACCCGAC GCCTGACTCC 
TTTTTGGATC GTTTTCATGG AGCTGAGCTT AAGGAAGTAT CCAGCCAGGA AAGGAATGCC 
CAGCCCAACC CAGGAGGACA GGAACCACCA GAGGGAGGGA AAGGCAGGAA GAAGGATCCC 
ATTGTGGTGG ACCCCTCCAG CAACATCTAC TACCGCTGGC TGACTGCCAT CGCCCTCCCG 
GTCTTCTATA ACTGGTGTCT ACTTGTGTGC AGGGCCTGTT TTGATGAGCT ACAGTCAGAA 
CACCTGACAC TGTGGCTGGT CCTGGACTAC TCTGCAGATG CCCTGTATGT TGTGGACATG 
CTGGTTCGTG CCCGGACAGG TTTCCTTGAA CAAGGCCTAA TGGTCAGGGA TACCAAGAGG 
CTGTGGAAAC ATTACACAAA GACCTTGCAC TTCAAGCTGG ACATCCTGTC TCTCATCCCC 
ACAGACCTGG CTTATTTGAA GTTGGGCATG AACTACCCGG AACTGAGGTT CAATCGCCTC 
CTGAGGTTCT CTCGGCTCTT TGAGTTCTTT GACCGCACGG AGACAAGGAC CAACTACCCC 
AATGTGTTCA GGATAGGGAA CTTGGTTCTG TACACCCTCA TTATCATCCA CTGGAACGCC 
TGCATCTACT TTGCCATTTC CAAGTTCATC GGTTTTGGAA CAGATTCCTG GGTCTATCCA 
AACACCTCCA AGCCGGAGTA TGGACGCCTC TCCAGGAAGT ACATTTACAG CCTCTACTGG 
TCCACCTTGA CCCTGACCAC CATTGGGGAG ACCCCGCCCC CCGTGAAGGA TGAGGAGTAT 
CTCTTTGTGG TCATAGACTT CCTGGTGGGC GTCCTGATCT TCGCCACTAT AGTAGGCAAT 
GTGGGCTCCA TGATTTCCAA CATGAACGCT TCCCGGGCGG AGTTCCAGGC TAAGATAGAT 
TCCATCAAGC AGTACATGCA GTTCCGGAAG GTAACCAAGG ACTTGGAGAC TCGGGTTATC 
CGGTGGTTTG ACTATCTGTG GGCTAACAGG AAGACGGTGG ATGAAAAGGA AGTGCTCAAA 
AACCTCCCAG ACAAGCTGAA GGCCGAGATC GCCATCAACG TGCACCTGGA CACACTGAAG 
AAAGTCCGAA TCTTCCAGGA CTGCGAGGCA GGGCTTCTGG TGGAGCTGGT GCTGAAGCTT 
CGTCCTGCTG TGTTCAGCCC TGGGGACTAC ATTTGCAAAA AGGGGGACAT TGGAAGGGAG 
ATGTACATCA TCAAGGAGGG CAAGCTGGCT GTCGTGGCTG ACGATGGGGT CACCCAGTTT 
GTGGTCCTCA GTGATGGCAG TTACTTTGGG GAGATCAGCA TCTTAAACAT TAAGGGGAGC 
AAGTCGGGGA ACCGCAGGAC AGCCAACATC AGGAGCATCG GCTACTCGGA CCTGTTCTGC 
CTCTCCAAGG ATGACTTGAT GGAGACCCTC ACGGAGTACC CGGATGCTAA GAGGGCTCTG 
GAGGAAAAGG GTCGGCAGAT TCTGATGAAG GACAACCTAA TCGATGACGA CCTGGTGACG 
GCCAGGGCAG ATGCCAGGAA CATCGAGGAG AAGGTGGAGT ACCTGGAGTC ATCCTTGGAC 
GGCCTGCAGA CGAGGTTCGC CCGACTCCTG GCTGAGTACA GTGCCTCCCA GATGAAGCTG 
AAACAGCGGC TCAGCCAGCT GGAGAGCCAG ATGACCAGGA GGGGTCATGG CTTCTCACCT 
GACAGGGAGA ATTCCGAGGA TGCTTCAAAA GCTGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008765265.1
CDS	388..2286
Translation	MAKVNTQCSQPSPRQLSVKDAADRDLDQVENGLGRVSRFIISIRAWAARHLHHEDPTPDSFLDRFHGAELKEVSSQERNAQPNPGGQEPPEGGKGRKKDPIVVDPSSNIYYRWLTAIALPVFYNWCLLVCRACFDELQSEHLTLWLVLDYSADALYVVDMLVRARTGFLEQGLMVRDTKRLWKHYTKTLHFKLDILSLIPTDLAYLKLGMNYPELRFNRLLRFSRLFEFFDRTETRTNYPNVFRIGNLVLYTLIIIHWNACIYFAISKFIGFGTDSWVYPNTSKPEYGRLSRKYIYSLYWSTLTLTTIGETPPPVKDEEYLFVVIDFLVGVLIFATIVGNVGSMISNMNASRAEFQAKIDSIKQYMQFRKVTKDLETRVIRWFDYLWANRKTVDEKEVLKNLPDKLKAEIAINVHLDTLKKVRIFQDCEAGLLVELVLKLRPAVFSPGDYICKKGDIGREMYIIKEGKLAVVADDGVTQFVVLSDGSYFGEISILNIKGSKSGNRRTANIRSIGYSDLFCLSKDDLMETLTEYPDAKRALEEKGRQILMKDNLIDDDLVTARADARNIEEKVEYLESSLDGLQTRFARLLAEYSASQMKLKQRLSQLESQMTRRGHGFSPDRENSEDASKAD

Target ORF information:

RefSeq Version	XM_008767043.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus cyclic nucleotide gated channel alpha 3 (Cnga3), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008767043.2

ORF Insert Sequence:

ATGGCCAAGG TCAATACCCA ATGCTCACAG CCCTCCCCGA GACAACTTTC AGTAAAGGAC 
GCCGCAGACA GAGACCTCGA TCAAGTGGAG AATGGCCTCG GCAGGGTGTC ACGCTTCATC 
ATCTCCATTC GTGCGTGGGC CGCCAGGCAC TTACACCATG AAGACCCGAC GCCTGACTCC 
TTTTTGGATC GTTTTCATGG AGCTGAGCTT AAGGAAGTAT CCAGCCAGGA AAGGAATGCC 
CAGCCCAACC CAGGAGGACA GGAACCACCA GAGGGAGGGA AAGGCAGGAA GAAGGATCCC 
ATTGTGGTGG ACCCCTCCAG CAACATCTAC TACCGCTGGC TGACTGCCAT CGCCCTCCCG 
GTCTTCTATA ACTGGTGTCT ACTTGTGTGC AGGGCCTGTT TTGATGAGCT ACAGTCAGAA 
CACCTGACAC TGTGGCTGGT CCTGGACTAC TCTGCAGATG CCCTGTATGT TGTGGACATG 
CTGGTTCGTG CCCGGACAGG TTTCCTTGAA CAAGGCCTAA TGGTCAGGGA TACCAAGAGG 
CTGTGGAAAC ATTACACAAA GACCTTGCAC TTCAAGCTGG ACATCCTGTC TCTCATCCCC 
ACAGACCTGG CTTATTTGAA GTTGGGCATG AACTACCCGG AACTGAGGTT CAATCGCCTC 
CTGAGGTTCT CTCGGCTCTT TGAGTTCTTT GACCGCACGG AGACAAGGAC CAACTACCCC 
AATGTGTTCA GGATAGGGAA CTTGGTTCTG TACACCCTCA TTATCATCCA CTGGAACGCC 
TGCATCTACT TTGCCATTTC CAAGTTCATC GGTTTTGGAA CAGATTCCTG GGTCTATCCA 
AACACCTCCA AGCCGGAGTA TGGACGCCTC TCCAGGAAGT ACATTTACAG CCTCTACTGG 
TCCACCTTGA CCCTGACCAC CATTGGGGAG ACCCCGCCCC CCGTGAAGGA TGAGGAGTAT 
CTCTTTGTGG TCATAGACTT CCTGGTGGGC GTCCTGATCT TCGCCACTAT AGTAGGCAAT 
GTGGGCTCCA TGATTTCCAA CATGAACGCT TCCCGGGCGG AGTTCCAGGC TAAGATAGAT 
TCCATCAAGC AGTACATGCA GTTCCGGAAG GTAACCAAGG ACTTGGAGAC TCGGGTTATC 
CGGTGGTTTG ACTATCTGTG GGCTAACAGG AAGACGGTGG ATGAAAAGGA AGTGCTCAAA 
AACCTCCCAG ACAAGCTGAA GGCCGAGATC GCCATCAACG TGCACCTGGA CACACTGAAG 
AAAGTCCGAA TCTTCCAGGA CTGCGAGGCA GGGCTTCTGG TGGAGCTGGT GCTGAAGCTT 
CGTCCTGCTG TGTTCAGCCC TGGGGACTAC ATTTGCAAAA AGGGGGACAT TGGAAGGGAG 
ATGTACATCA TCAAGGAGGG CAAGCTGGCT GTCGTGGCTG ACGATGGGGT CACCCAGTTT 
GTGGTCCTCA GTGATGGCAG TTACTTTGGG GAGATCAGCA TCTTAAACAT TAAGGGGAGC 
AAGTCGGGGA ACCGCAGGAC AGCCAACATC AGGAGCATCG GCTACTCGGA CCTGTTCTGC 
CTCTCCAAGG ATGACTTGAT GGAGACCCTC ACGGAGTACC CGGATGCTAA GAGGGCTCTG 
GAGGAAAAGG GTCGGCAGAT TCTGATGAAG GACAACCTAA TCGATGACGA CCTGGTGACG 
GCCAGGGCAG ATGCCAGGAA CATCGAGGAG AAGGTGGAGT ACCTGGAGTC ATCCTTGGAC 
GGCCTGCAGA CGAGGTTCGC CCGACTCCTG GCTGAGTACA GTGCCTCCCA GATGAAGCTG 
AAACAGCGGC TCAGCCAGCT GGAGAGCCAG ATGACCAGGA GGGGTCATGG CTTCTCACCT 
GACAGGGAGA ATTCCGAGGA TGCTTCAAAA GCTGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cnga3 ( XM_017596680.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017452169.1 Rattus norvegicus cyclic nucleotide gated channel alpha 3 (Cnga3), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa43863
Clone ID Related Accession (Same CDS sequence)	XM_017596680.1 , XM_008767042.2
Accession Version	XM_017596680.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2013bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	cyclic nucleotide-gated cation channel alpha-3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005108.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAGG TCAATACCCA ATGCTCACAG CCCTCCCCGA GACAACTTTC AGTAAAGGAC 
GCCGCAGACA GAGACCTCGA TCAAGTGGAG AATGGCCTCG GCAGGTCTCA CTCACCATGT 
GAGGAGACAT CTTCCACAGT GCATCAGGGG ATCGTCATGG AGACCGGAGA ACTGGCTGGA 
GCCGCACAGA GTTTATTCAC AAGCCAGGGG CCAGCCAGGG TGTCACGCTT CATCATCTCC 
ATTCGTGCGT GGGCCGCCAG GCACTTACAC CATGAAGACC CGACGCCTGA CTCCTTTTTG 
GATCGTTTTC ATGGAGCTGA GCTTAAGGAA GTATCCAGCC AGGAAAGGAA TGCCCAGCCC 
AACCCAGGAG GACAGGAACC ACCAGAGGGA GGGAAAGGCA GGAAGAAGGA TCCCATTGTG 
GTGGACCCCT CCAGCAACAT CTACTACCGC TGGCTGACTG CCATCGCCCT CCCGGTCTTC 
TATAACTGGT GTCTACTTGT GTGCAGGGCC TGTTTTGATG AGCTACAGTC AGAACACCTG 
ACACTGTGGC TGGTCCTGGA CTACTCTGCA GATGCCCTGT ATGTTGTGGA CATGCTGGTT 
CGTGCCCGGA CAGGTTTCCT TGAACAAGGC CTAATGGTCA GGGATACCAA GAGGCTGTGG 
AAACATTACA CAAAGACCTT GCACTTCAAG CTGGACATCC TGTCTCTCAT CCCCACAGAC 
CTGGCTTATT TGAAGTTGGG CATGAACTAC CCGGAACTGA GGTTCAATCG CCTCCTGAGG 
TTCTCTCGGC TCTTTGAGTT CTTTGACCGC ACGGAGACAA GGACCAACTA CCCCAATGTG 
TTCAGGATAG GGAACTTGGT TCTGTACACC CTCATTATCA TCCACTGGAA CGCCTGCATC 
TACTTTGCCA TTTCCAAGTT CATCGGTTTT GGAACAGATT CCTGGGTCTA TCCAAACACC 
TCCAAGCCGG AGTATGGACG CCTCTCCAGG AAGTACATTT ACAGCCTCTA CTGGTCCACC 
TTGACCCTGA CCACCATTGG GGAGACCCCG CCCCCCGTGA AGGATGAGGA GTATCTCTTT 
GTGGTCATAG ACTTCCTGGT GGGCGTCCTG ATCTTCGCCA CTATAGTAGG CAATGTGGGC 
TCCATGATTT CCAACATGAA CGCTTCCCGG GCGGAGTTCC AGGCTAAGAT AGATTCCATC 
AAGCAGTACA TGCAGTTCCG GAAGGTAACC AAGGACTTGG AGACTCGGGT TATCCGGTGG 
TTTGACTATC TGTGGGCTAA CAGGAAGACG GTGGATGAAA AGGAAGTGCT CAAAAACCTC 
CCAGACAAGC TGAAGGCCGA GATCGCCATC AACGTGCACC TGGACACACT GAAGAAAGTC 
CGAATCTTCC AGGACTGCGA GGCAGGGCTT CTGGTGGAGC TGGTGCTGAA GCTTCGTCCT 
GCTGTGTTCA GCCCTGGGGA CTACATTTGC AAAAAGGGGG ACATTGGAAG GGAGATGTAC 
ATCATCAAGG AGGGCAAGCT GGCTGTCGTG GCTGACGATG GGGTCACCCA GTTTGTGGTC 
CTCAGTGATG GCAGTTACTT TGGGGAGATC AGCATCTTAA ACATTAAGGG GAGCAAGTCG 
GGGAACCGCA GGACAGCCAA CATCAGGAGC ATCGGCTACT CGGACCTGTT CTGCCTCTCC 
AAGGATGACT TGATGGAGAC CCTCACGGAG TACCCGGATG CTAAGAGGGC TCTGGAGGAA 
AAGGGTCGGC AGATTCTGAT GAAGGACAAC CTAATCGATG ACGACCTGGT GACGGCCAGG 
GCAGATGCCA GGAACATCGA GGAGAAGGTG GAGTACCTGG AGTCATCCTT GGACGGCCTG 
CAGACGAGGT TCGCCCGACT CCTGGCTGAG TACAGTGCCT CCCAGATGAA GCTGAAACAG 
CGGCTCAGCC AGCTGGAGAG CCAGATGACC AGGAGGGGTC ATGGCTTCTC ACCTGACAGG 
GAGAATTCCG AGGATGCTTC AAAAGCTGAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017452169.1
CDS	1414..3426
Translation	MAKVNTQCSQPSPRQLSVKDAADRDLDQVENGLGRSHSPCEETSSTVHQGIVMETGELAGAAQSLFTSQGPARVSRFIISIRAWAARHLHHEDPTPDSFLDRFHGAELKEVSSQERNAQPNPGGQEPPEGGKGRKKDPIVVDPSSNIYYRWLTAIALPVFYNWCLLVCRACFDELQSEHLTLWLVLDYSADALYVVDMLVRARTGFLEQGLMVRDTKRLWKHYTKTLHFKLDILSLIPTDLAYLKLGMNYPELRFNRLLRFSRLFEFFDRTETRTNYPNVFRIGNLVLYTLIIIHWNACIYFAISKFIGFGTDSWVYPNTSKPEYGRLSRKYIYSLYWSTLTLTTIGETPPPVKDEEYLFVVIDFLVGVLIFATIVGNVGSMISNMNASRAEFQAKIDSIKQYMQFRKVTKDLETRVIRWFDYLWANRKTVDEKEVLKNLPDKLKAEIAINVHLDTLKKVRIFQDCEAGLLVELVLKLRPAVFSPGDYICKKGDIGREMYIIKEGKLAVVADDGVTQFVVLSDGSYFGEISILNIKGSKSGNRRTANIRSIGYSDLFCLSKDDLMETLTEYPDAKRALEEKGRQILMKDNLIDDDLVTARADARNIEEKVEYLESSLDGLQTRFARLLAEYSASQMKLKQRLSQLESQMTRRGHGFSPDRENSEDASKAD

Target ORF information:

RefSeq Version	XM_017596680.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus cyclic nucleotide gated channel alpha 3 (Cnga3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017596680.1

ORF Insert Sequence:

ATGGCCAAGG TCAATACCCA ATGCTCACAG CCCTCCCCGA GACAACTTTC AGTAAAGGAC 
GCCGCAGACA GAGACCTCGA TCAAGTGGAG AATGGCCTCG GCAGGTCTCA CTCACCATGT 
GAGGAGACAT CTTCCACAGT GCATCAGGGG ATCGTCATGG AGACCGGAGA ACTGGCTGGA 
GCCGCACAGA GTTTATTCAC AAGCCAGGGG CCAGCCAGGG TGTCACGCTT CATCATCTCC 
ATTCGTGCGT GGGCCGCCAG GCACTTACAC CATGAAGACC CGACGCCTGA CTCCTTTTTG 
GATCGTTTTC ATGGAGCTGA GCTTAAGGAA GTATCCAGCC AGGAAAGGAA TGCCCAGCCC 
AACCCAGGAG GACAGGAACC ACCAGAGGGA GGGAAAGGCA GGAAGAAGGA TCCCATTGTG 
GTGGACCCCT CCAGCAACAT CTACTACCGC TGGCTGACTG CCATCGCCCT CCCGGTCTTC 
TATAACTGGT GTCTACTTGT GTGCAGGGCC TGTTTTGATG AGCTACAGTC AGAACACCTG 
ACACTGTGGC TGGTCCTGGA CTACTCTGCA GATGCCCTGT ATGTTGTGGA CATGCTGGTT 
CGTGCCCGGA CAGGTTTCCT TGAACAAGGC CTAATGGTCA GGGATACCAA GAGGCTGTGG 
AAACATTACA CAAAGACCTT GCACTTCAAG CTGGACATCC TGTCTCTCAT CCCCACAGAC 
CTGGCTTATT TGAAGTTGGG CATGAACTAC CCGGAACTGA GGTTCAATCG CCTCCTGAGG 
TTCTCTCGGC TCTTTGAGTT CTTTGACCGC ACGGAGACAA GGACCAACTA CCCCAATGTG 
TTCAGGATAG GGAACTTGGT TCTGTACACC CTCATTATCA TCCACTGGAA CGCCTGCATC 
TACTTTGCCA TTTCCAAGTT CATCGGTTTT GGAACAGATT CCTGGGTCTA TCCAAACACC 
TCCAAGCCGG AGTATGGACG CCTCTCCAGG AAGTACATTT ACAGCCTCTA CTGGTCCACC 
TTGACCCTGA CCACCATTGG GGAGACCCCG CCCCCCGTGA AGGATGAGGA GTATCTCTTT 
GTGGTCATAG ACTTCCTGGT GGGCGTCCTG ATCTTCGCCA CTATAGTAGG CAATGTGGGC 
TCCATGATTT CCAACATGAA CGCTTCCCGG GCGGAGTTCC AGGCTAAGAT AGATTCCATC 
AAGCAGTACA TGCAGTTCCG GAAGGTAACC AAGGACTTGG AGACTCGGGT TATCCGGTGG 
TTTGACTATC TGTGGGCTAA CAGGAAGACG GTGGATGAAA AGGAAGTGCT CAAAAACCTC 
CCAGACAAGC TGAAGGCCGA GATCGCCATC AACGTGCACC TGGACACACT GAAGAAAGTC 
CGAATCTTCC AGGACTGCGA GGCAGGGCTT CTGGTGGAGC TGGTGCTGAA GCTTCGTCCT 
GCTGTGTTCA GCCCTGGGGA CTACATTTGC AAAAAGGGGG ACATTGGAAG GGAGATGTAC 
ATCATCAAGG AGGGCAAGCT GGCTGTCGTG GCTGACGATG GGGTCACCCA GTTTGTGGTC 
CTCAGTGATG GCAGTTACTT TGGGGAGATC AGCATCTTAA ACATTAAGGG GAGCAAGTCG 
GGGAACCGCA GGACAGCCAA CATCAGGAGC ATCGGCTACT CGGACCTGTT CTGCCTCTCC 
AAGGATGACT TGATGGAGAC CCTCACGGAG TACCCGGATG CTAAGAGGGC TCTGGAGGAA 
AAGGGTCGGC AGATTCTGAT GAAGGACAAC CTAATCGATG ACGACCTGGT GACGGCCAGG 
GCAGATGCCA GGAACATCGA GGAGAAGGTG GAGTACCTGG AGTCATCCTT GGACGGCCTG 
CAGACGAGGT TCGCCCGACT CCTGGCTGAG TACAGTGCCT CCCAGATGAA GCTGAAACAG 
CGGCTCAGCC AGCTGGAGAG CCAGATGACC AGGAGGGGTC ATGGCTTCTC ACCTGACAGG 
GAGAATTCCG AGGATGCTTC AAAAGCTGAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cnga3 ( XM_008767042.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008765264.1 Rattus norvegicus cyclic nucleotide gated channel alpha 3 (Cnga3), transcript variant X1, mRNA.

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CloneID	ORa43863
Clone ID Related Accession (Same CDS sequence)	XM_017596680.1 , XM_008767042.2
Accession Version	XM_008767042.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2013bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	cyclic nucleotide-gated cation channel alpha-3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005108.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008767042.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAGG TCAATACCCA ATGCTCACAG CCCTCCCCGA GACAACTTTC AGTAAAGGAC 
GCCGCAGACA GAGACCTCGA TCAAGTGGAG AATGGCCTCG GCAGGTCTCA CTCACCATGT 
GAGGAGACAT CTTCCACAGT GCATCAGGGG ATCGTCATGG AGACCGGAGA ACTGGCTGGA 
GCCGCACAGA GTTTATTCAC AAGCCAGGGG CCAGCCAGGG TGTCACGCTT CATCATCTCC 
ATTCGTGCGT GGGCCGCCAG GCACTTACAC CATGAAGACC CGACGCCTGA CTCCTTTTTG 
GATCGTTTTC ATGGAGCTGA GCTTAAGGAA GTATCCAGCC AGGAAAGGAA TGCCCAGCCC 
AACCCAGGAG GACAGGAACC ACCAGAGGGA GGGAAAGGCA GGAAGAAGGA TCCCATTGTG 
GTGGACCCCT CCAGCAACAT CTACTACCGC TGGCTGACTG CCATCGCCCT CCCGGTCTTC 
TATAACTGGT GTCTACTTGT GTGCAGGGCC TGTTTTGATG AGCTACAGTC AGAACACCTG 
ACACTGTGGC TGGTCCTGGA CTACTCTGCA GATGCCCTGT ATGTTGTGGA CATGCTGGTT 
CGTGCCCGGA CAGGTTTCCT TGAACAAGGC CTAATGGTCA GGGATACCAA GAGGCTGTGG 
AAACATTACA CAAAGACCTT GCACTTCAAG CTGGACATCC TGTCTCTCAT CCCCACAGAC 
CTGGCTTATT TGAAGTTGGG CATGAACTAC CCGGAACTGA GGTTCAATCG CCTCCTGAGG 
TTCTCTCGGC TCTTTGAGTT CTTTGACCGC ACGGAGACAA GGACCAACTA CCCCAATGTG 
TTCAGGATAG GGAACTTGGT TCTGTACACC CTCATTATCA TCCACTGGAA CGCCTGCATC 
TACTTTGCCA TTTCCAAGTT CATCGGTTTT GGAACAGATT CCTGGGTCTA TCCAAACACC 
TCCAAGCCGG AGTATGGACG CCTCTCCAGG AAGTACATTT ACAGCCTCTA CTGGTCCACC 
TTGACCCTGA CCACCATTGG GGAGACCCCG CCCCCCGTGA AGGATGAGGA GTATCTCTTT 
GTGGTCATAG ACTTCCTGGT GGGCGTCCTG ATCTTCGCCA CTATAGTAGG CAATGTGGGC 
TCCATGATTT CCAACATGAA CGCTTCCCGG GCGGAGTTCC AGGCTAAGAT AGATTCCATC 
AAGCAGTACA TGCAGTTCCG GAAGGTAACC AAGGACTTGG AGACTCGGGT TATCCGGTGG 
TTTGACTATC TGTGGGCTAA CAGGAAGACG GTGGATGAAA AGGAAGTGCT CAAAAACCTC 
CCAGACAAGC TGAAGGCCGA GATCGCCATC AACGTGCACC TGGACACACT GAAGAAAGTC 
CGAATCTTCC AGGACTGCGA GGCAGGGCTT CTGGTGGAGC TGGTGCTGAA GCTTCGTCCT 
GCTGTGTTCA GCCCTGGGGA CTACATTTGC AAAAAGGGGG ACATTGGAAG GGAGATGTAC 
ATCATCAAGG AGGGCAAGCT GGCTGTCGTG GCTGACGATG GGGTCACCCA GTTTGTGGTC 
CTCAGTGATG GCAGTTACTT TGGGGAGATC AGCATCTTAA ACATTAAGGG GAGCAAGTCG 
GGGAACCGCA GGACAGCCAA CATCAGGAGC ATCGGCTACT CGGACCTGTT CTGCCTCTCC 
AAGGATGACT TGATGGAGAC CCTCACGGAG TACCCGGATG CTAAGAGGGC TCTGGAGGAA 
AAGGGTCGGC AGATTCTGAT GAAGGACAAC CTAATCGATG ACGACCTGGT GACGGCCAGG 
GCAGATGCCA GGAACATCGA GGAGAAGGTG GAGTACCTGG AGTCATCCTT GGACGGCCTG 
CAGACGAGGT TCGCCCGACT CCTGGCTGAG TACAGTGCCT CCCAGATGAA GCTGAAACAG 
CGGCTCAGCC AGCTGGAGAG CCAGATGACC AGGAGGGGTC ATGGCTTCTC ACCTGACAGG 
GAGAATTCCG AGGATGCTTC AAAAGCTGAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008765264.1
CDS	389..2401
Translation	MAKVNTQCSQPSPRQLSVKDAADRDLDQVENGLGRSHSPCEETSSTVHQGIVMETGELAGAAQSLFTSQGPARVSRFIISIRAWAARHLHHEDPTPDSFLDRFHGAELKEVSSQERNAQPNPGGQEPPEGGKGRKKDPIVVDPSSNIYYRWLTAIALPVFYNWCLLVCRACFDELQSEHLTLWLVLDYSADALYVVDMLVRARTGFLEQGLMVRDTKRLWKHYTKTLHFKLDILSLIPTDLAYLKLGMNYPELRFNRLLRFSRLFEFFDRTETRTNYPNVFRIGNLVLYTLIIIHWNACIYFAISKFIGFGTDSWVYPNTSKPEYGRLSRKYIYSLYWSTLTLTTIGETPPPVKDEEYLFVVIDFLVGVLIFATIVGNVGSMISNMNASRAEFQAKIDSIKQYMQFRKVTKDLETRVIRWFDYLWANRKTVDEKEVLKNLPDKLKAEIAINVHLDTLKKVRIFQDCEAGLLVELVLKLRPAVFSPGDYICKKGDIGREMYIIKEGKLAVVADDGVTQFVVLSDGSYFGEISILNIKGSKSGNRRTANIRSIGYSDLFCLSKDDLMETLTEYPDAKRALEEKGRQILMKDNLIDDDLVTARADARNIEEKVEYLESSLDGLQTRFARLLAEYSASQMKLKQRLSQLESQMTRRGHGFSPDRENSEDASKAD

Target ORF information:

RefSeq Version	XM_008767042.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus cyclic nucleotide gated channel alpha 3 (Cnga3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008767042.2

ORF Insert Sequence:

ATGGCCAAGG TCAATACCCA ATGCTCACAG CCCTCCCCGA GACAACTTTC AGTAAAGGAC 
GCCGCAGACA GAGACCTCGA TCAAGTGGAG AATGGCCTCG GCAGGTCTCA CTCACCATGT 
GAGGAGACAT CTTCCACAGT GCATCAGGGG ATCGTCATGG AGACCGGAGA ACTGGCTGGA 
GCCGCACAGA GTTTATTCAC AAGCCAGGGG CCAGCCAGGG TGTCACGCTT CATCATCTCC 
ATTCGTGCGT GGGCCGCCAG GCACTTACAC CATGAAGACC CGACGCCTGA CTCCTTTTTG 
GATCGTTTTC ATGGAGCTGA GCTTAAGGAA GTATCCAGCC AGGAAAGGAA TGCCCAGCCC 
AACCCAGGAG GACAGGAACC ACCAGAGGGA GGGAAAGGCA GGAAGAAGGA TCCCATTGTG 
GTGGACCCCT CCAGCAACAT CTACTACCGC TGGCTGACTG CCATCGCCCT CCCGGTCTTC 
TATAACTGGT GTCTACTTGT GTGCAGGGCC TGTTTTGATG AGCTACAGTC AGAACACCTG 
ACACTGTGGC TGGTCCTGGA CTACTCTGCA GATGCCCTGT ATGTTGTGGA CATGCTGGTT 
CGTGCCCGGA CAGGTTTCCT TGAACAAGGC CTAATGGTCA GGGATACCAA GAGGCTGTGG 
AAACATTACA CAAAGACCTT GCACTTCAAG CTGGACATCC TGTCTCTCAT CCCCACAGAC 
CTGGCTTATT TGAAGTTGGG CATGAACTAC CCGGAACTGA GGTTCAATCG CCTCCTGAGG 
TTCTCTCGGC TCTTTGAGTT CTTTGACCGC ACGGAGACAA GGACCAACTA CCCCAATGTG 
TTCAGGATAG GGAACTTGGT TCTGTACACC CTCATTATCA TCCACTGGAA CGCCTGCATC 
TACTTTGCCA TTTCCAAGTT CATCGGTTTT GGAACAGATT CCTGGGTCTA TCCAAACACC 
TCCAAGCCGG AGTATGGACG CCTCTCCAGG AAGTACATTT ACAGCCTCTA CTGGTCCACC 
TTGACCCTGA CCACCATTGG GGAGACCCCG CCCCCCGTGA AGGATGAGGA GTATCTCTTT 
GTGGTCATAG ACTTCCTGGT GGGCGTCCTG ATCTTCGCCA CTATAGTAGG CAATGTGGGC 
TCCATGATTT CCAACATGAA CGCTTCCCGG GCGGAGTTCC AGGCTAAGAT AGATTCCATC 
AAGCAGTACA TGCAGTTCCG GAAGGTAACC AAGGACTTGG AGACTCGGGT TATCCGGTGG 
TTTGACTATC TGTGGGCTAA CAGGAAGACG GTGGATGAAA AGGAAGTGCT CAAAAACCTC 
CCAGACAAGC TGAAGGCCGA GATCGCCATC AACGTGCACC TGGACACACT GAAGAAAGTC 
CGAATCTTCC AGGACTGCGA GGCAGGGCTT CTGGTGGAGC TGGTGCTGAA GCTTCGTCCT 
GCTGTGTTCA GCCCTGGGGA CTACATTTGC AAAAAGGGGG ACATTGGAAG GGAGATGTAC 
ATCATCAAGG AGGGCAAGCT GGCTGTCGTG GCTGACGATG GGGTCACCCA GTTTGTGGTC 
CTCAGTGATG GCAGTTACTT TGGGGAGATC AGCATCTTAA ACATTAAGGG GAGCAAGTCG 
GGGAACCGCA GGACAGCCAA CATCAGGAGC ATCGGCTACT CGGACCTGTT CTGCCTCTCC 
AAGGATGACT TGATGGAGAC CCTCACGGAG TACCCGGATG CTAAGAGGGC TCTGGAGGAA 
AAGGGTCGGC AGATTCTGAT GAAGGACAAC CTAATCGATG ACGACCTGGT GACGGCCAGG 
GCAGATGCCA GGAACATCGA GGAGAAGGTG GAGTACCTGG AGTCATCCTT GGACGGCCTG 
CAGACGAGGT TCGCCCGACT CCTGGCTGAG TACAGTGCCT CCCAGATGAA GCTGAAACAG 
CGGCTCAGCC AGCTGGAGAG CCAGATGACC AGGAGGGGTC ATGGCTTCTC ACCTGACAGG 
GAGAATTCCG AGGATGCTTC AAAAGCTGAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cnga3 ( XM_017596681.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017452170.1 Rattus norvegicus cyclic nucleotide gated channel alpha 3 (Cnga3), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa62897
Clone ID Related Accession (Same CDS sequence)	XM_017596681.1
Accession Version	XM_017596681.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2010bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	cyclic nucleotide-gated cation channel alpha-3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005108.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAGG TCAATACCCA ATGCTCACAG CCCTCCCCGA GACAACTTTC AGTAAAGGAC 
GCCGCAGACA GAGACCTCGA TCAAGTGGAG AATGGCCTCG GCAGGTCTCA CTCACCATGT 
GAGGAGACAT CTTCCACAGT GCATCAGGGG ATCGTCATGG AGACCGGAGA ACTGGCTGGA 
GCCGCACAGA GTTTATTCAC AAGCCAGGGG CCAGCCAGGG TGTCACGCTT CATCATCTCC 
ATTCGTGCGT GGGCCGCCAG GCACTTACAC CATGAAGACC CGACGCCTGA CTCCTTTTTG 
GATCGTTTTC ATGGAGCTGA GCTTAAGGAA GTATCCAGCC AGGAAAGGAA TGCCCAGCCC 
AACCCAGGAG GACAGGAACC ACCAGAGGGA GGGAAAGGGA AGAAGGATCC CATTGTGGTG 
GACCCCTCCA GCAACATCTA CTACCGCTGG CTGACTGCCA TCGCCCTCCC GGTCTTCTAT 
AACTGGTGTC TACTTGTGTG CAGGGCCTGT TTTGATGAGC TACAGTCAGA ACACCTGACA 
CTGTGGCTGG TCCTGGACTA CTCTGCAGAT GCCCTGTATG TTGTGGACAT GCTGGTTCGT 
GCCCGGACAG GTTTCCTTGA ACAAGGCCTA ATGGTCAGGG ATACCAAGAG GCTGTGGAAA 
CATTACACAA AGACCTTGCA CTTCAAGCTG GACATCCTGT CTCTCATCCC CACAGACCTG 
GCTTATTTGA AGTTGGGCAT GAACTACCCG GAACTGAGGT TCAATCGCCT CCTGAGGTTC 
TCTCGGCTCT TTGAGTTCTT TGACCGCACG GAGACAAGGA CCAACTACCC CAATGTGTTC 
AGGATAGGGA ACTTGGTTCT GTACACCCTC ATTATCATCC ACTGGAACGC CTGCATCTAC 
TTTGCCATTT CCAAGTTCAT CGGTTTTGGA ACAGATTCCT GGGTCTATCC AAACACCTCC 
AAGCCGGAGT ATGGACGCCT CTCCAGGAAG TACATTTACA GCCTCTACTG GTCCACCTTG 
ACCCTGACCA CCATTGGGGA GACCCCGCCC CCCGTGAAGG ATGAGGAGTA TCTCTTTGTG 
GTCATAGACT TCCTGGTGGG CGTCCTGATC TTCGCCACTA TAGTAGGCAA TGTGGGCTCC 
ATGATTTCCA ACATGAACGC TTCCCGGGCG GAGTTCCAGG CTAAGATAGA TTCCATCAAG 
CAGTACATGC AGTTCCGGAA GGTAACCAAG GACTTGGAGA CTCGGGTTAT CCGGTGGTTT 
GACTATCTGT GGGCTAACAG GAAGACGGTG GATGAAAAGG AAGTGCTCAA AAACCTCCCA 
GACAAGCTGA AGGCCGAGAT CGCCATCAAC GTGCACCTGG ACACACTGAA GAAAGTCCGA 
ATCTTCCAGG ACTGCGAGGC AGGGCTTCTG GTGGAGCTGG TGCTGAAGCT TCGTCCTGCT 
GTGTTCAGCC CTGGGGACTA CATTTGCAAA AAGGGGGACA TTGGAAGGGA GATGTACATC 
ATCAAGGAGG GCAAGCTGGC TGTCGTGGCT GACGATGGGG TCACCCAGTT TGTGGTCCTC 
AGTGATGGCA GTTACTTTGG GGAGATCAGC ATCTTAAACA TTAAGGGGAG CAAGTCGGGG 
AACCGCAGGA CAGCCAACAT CAGGAGCATC GGCTACTCGG ACCTGTTCTG CCTCTCCAAG 
GATGACTTGA TGGAGACCCT CACGGAGTAC CCGGATGCTA AGAGGGCTCT GGAGGAAAAG 
GGTCGGCAGA TTCTGATGAA GGACAACCTA ATCGATGACG ACCTGGTGAC GGCCAGGGCA 
GATGCCAGGA ACATCGAGGA GAAGGTGGAG TACCTGGAGT CATCCTTGGA CGGCCTGCAG 
ACGAGGTTCG CCCGACTCCT GGCTGAGTAC AGTGCCTCCC AGATGAAGCT GAAACAGCGG 
CTCAGCCAGC TGGAGAGCCA GATGACCAGG AGGGGTCATG GCTTCTCACC TGACAGGGAG 
AATTCCGAGG ATGCTTCAAA AGCTGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017452170.1
CDS	389..2398
Translation	MAKVNTQCSQPSPRQLSVKDAADRDLDQVENGLGRSHSPCEETSSTVHQGIVMETGELAGAAQSLFTSQGPARVSRFIISIRAWAARHLHHEDPTPDSFLDRFHGAELKEVSSQERNAQPNPGGQEPPEGGKGKKDPIVVDPSSNIYYRWLTAIALPVFYNWCLLVCRACFDELQSEHLTLWLVLDYSADALYVVDMLVRARTGFLEQGLMVRDTKRLWKHYTKTLHFKLDILSLIPTDLAYLKLGMNYPELRFNRLLRFSRLFEFFDRTETRTNYPNVFRIGNLVLYTLIIIHWNACIYFAISKFIGFGTDSWVYPNTSKPEYGRLSRKYIYSLYWSTLTLTTIGETPPPVKDEEYLFVVIDFLVGVLIFATIVGNVGSMISNMNASRAEFQAKIDSIKQYMQFRKVTKDLETRVIRWFDYLWANRKTVDEKEVLKNLPDKLKAEIAINVHLDTLKKVRIFQDCEAGLLVELVLKLRPAVFSPGDYICKKGDIGREMYIIKEGKLAVVADDGVTQFVVLSDGSYFGEISILNIKGSKSGNRRTANIRSIGYSDLFCLSKDDLMETLTEYPDAKRALEEKGRQILMKDNLIDDDLVTARADARNIEEKVEYLESSLDGLQTRFARLLAEYSASQMKLKQRLSQLESQMTRRGHGFSPDRENSEDASKAD

Target ORF information:

RefSeq Version	XM_017596681.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus cyclic nucleotide gated channel alpha 3 (Cnga3), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017596681.1

ORF Insert Sequence:

ATGGCCAAGG TCAATACCCA ATGCTCACAG CCCTCCCCGA GACAACTTTC AGTAAAGGAC 
GCCGCAGACA GAGACCTCGA TCAAGTGGAG AATGGCCTCG GCAGGTCTCA CTCACCATGT 
GAGGAGACAT CTTCCACAGT GCATCAGGGG ATCGTCATGG AGACCGGAGA ACTGGCTGGA 
GCCGCACAGA GTTTATTCAC AAGCCAGGGG CCAGCCAGGG TGTCACGCTT CATCATCTCC 
ATTCGTGCGT GGGCCGCCAG GCACTTACAC CATGAAGACC CGACGCCTGA CTCCTTTTTG 
GATCGTTTTC ATGGAGCTGA GCTTAAGGAA GTATCCAGCC AGGAAAGGAA TGCCCAGCCC 
AACCCAGGAG GACAGGAACC ACCAGAGGGA GGGAAAGGGA AGAAGGATCC CATTGTGGTG 
GACCCCTCCA GCAACATCTA CTACCGCTGG CTGACTGCCA TCGCCCTCCC GGTCTTCTAT 
AACTGGTGTC TACTTGTGTG CAGGGCCTGT TTTGATGAGC TACAGTCAGA ACACCTGACA 
CTGTGGCTGG TCCTGGACTA CTCTGCAGAT GCCCTGTATG TTGTGGACAT GCTGGTTCGT 
GCCCGGACAG GTTTCCTTGA ACAAGGCCTA ATGGTCAGGG ATACCAAGAG GCTGTGGAAA 
CATTACACAA AGACCTTGCA CTTCAAGCTG GACATCCTGT CTCTCATCCC CACAGACCTG 
GCTTATTTGA AGTTGGGCAT GAACTACCCG GAACTGAGGT TCAATCGCCT CCTGAGGTTC 
TCTCGGCTCT TTGAGTTCTT TGACCGCACG GAGACAAGGA CCAACTACCC CAATGTGTTC 
AGGATAGGGA ACTTGGTTCT GTACACCCTC ATTATCATCC ACTGGAACGC CTGCATCTAC 
TTTGCCATTT CCAAGTTCAT CGGTTTTGGA ACAGATTCCT GGGTCTATCC AAACACCTCC 
AAGCCGGAGT ATGGACGCCT CTCCAGGAAG TACATTTACA GCCTCTACTG GTCCACCTTG 
ACCCTGACCA CCATTGGGGA GACCCCGCCC CCCGTGAAGG ATGAGGAGTA TCTCTTTGTG 
GTCATAGACT TCCTGGTGGG CGTCCTGATC TTCGCCACTA TAGTAGGCAA TGTGGGCTCC 
ATGATTTCCA ACATGAACGC TTCCCGGGCG GAGTTCCAGG CTAAGATAGA TTCCATCAAG 
CAGTACATGC AGTTCCGGAA GGTAACCAAG GACTTGGAGA CTCGGGTTAT CCGGTGGTTT 
GACTATCTGT GGGCTAACAG GAAGACGGTG GATGAAAAGG AAGTGCTCAA AAACCTCCCA 
GACAAGCTGA AGGCCGAGAT CGCCATCAAC GTGCACCTGG ACACACTGAA GAAAGTCCGA 
ATCTTCCAGG ACTGCGAGGC AGGGCTTCTG GTGGAGCTGG TGCTGAAGCT TCGTCCTGCT 
GTGTTCAGCC CTGGGGACTA CATTTGCAAA AAGGGGGACA TTGGAAGGGA GATGTACATC 
ATCAAGGAGG GCAAGCTGGC TGTCGTGGCT GACGATGGGG TCACCCAGTT TGTGGTCCTC 
AGTGATGGCA GTTACTTTGG GGAGATCAGC ATCTTAAACA TTAAGGGGAG CAAGTCGGGG 
AACCGCAGGA CAGCCAACAT CAGGAGCATC GGCTACTCGG ACCTGTTCTG CCTCTCCAAG 
GATGACTTGA TGGAGACCCT CACGGAGTAC CCGGATGCTA AGAGGGCTCT GGAGGAAAAG 
GGTCGGCAGA TTCTGATGAA GGACAACCTA ATCGATGACG ACCTGGTGAC GGCCAGGGCA 
GATGCCAGGA ACATCGAGGA GAAGGTGGAG TACCTGGAGT CATCCTTGGA CGGCCTGCAG 
ACGAGGTTCG CCCGACTCCT GGCTGAGTAC AGTGCCTCCC AGATGAAGCT GAAACAGCGG 
CTCAGCCAGC TGGAGAGCCA GATGACCAGG AGGGGTCATG GCTTCTCACC TGACAGGGAG 
AATTCCGAGG ATGCTTCAAA AGCTGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Cyclic nucleotide-gated channel subunit glycosylation regulates matrix metalloproteinase-dependent changes in channel gating.
Biochemistry52(46)8352-62(2013 Nov)
Meighan SE,Meighan PC,Rich ED,Brown RL,Varnum MD

Cyclic nucleotide-gated channel ?-3 (CNGA3) interacts with stereocilia tip-link cadherin 23 + exon 68 or alternatively with myosin VIIa, two proteins required for hair cell mechanotransduction.
The Journal of biological chemistry288(10)7215-29(2013 Mar)
Selvakumar D,Drescher MJ,Drescher DG

Calcium signalling remodelling and disease.
Biochemical Society transactions40(2)297-309(2012 Apr)
Berridge MJ

CNGA3 is expressed in inner ear hair cells and binds to an intracellular C-terminus domain of EMILIN1.
The Biochemical journal443(2)463-76(2012 Apr)
Selvakumar D,Drescher MJ,Dowdall JR,Khan KM,Hatfield JS,Ramakrishnan NA,Drescher DG

Mouse model resources for vision research.
Journal of ophthalmology2011391384(2011)
Won J,Shi LY,Hicks W,Wang J,Hurd R,Naggert JK,Chang B,Nishina PM

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Cnga3 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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