Nucb1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Nucb1
Entrez Gene ID	84595
Full Name	nucleobindin 1
Synonyms	Nucb
General protein information	Preferred Names nucleobindin 1 Names nucleobindin-1 CALNUC bone 63 kDa calcium-binding protein
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 1 Map Location: 1q22
Summary	extracellular calcium binding protein of the mineralized matrix of bone [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_053463.1	NP_445915.1	nucleobindin-1 precursor
XM_006229165.1	XP_006229227.1	nucleobindin-1 isoform X4
XM_006229163.1	XP_006229225.1	nucleobindin-1 isoform X3
XM_017589802.1	XP_017445291.1	nucleobindin-1 isoform X2
XM_006229164.3	XP_006229226.1	nucleobindin-1 isoform X3
XM_017589801.1	XP_017445290.1	nucleobindin-1 isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	NUCB1	NP_006175.2
Canis lupus familiaris (dog)	NUCB1	XP_541506.2
Danio rerio (zebrafish)	nucb1	NP_001038928.1
Xenopus tropicalis (tropical clawed frog)	nucb1	NP_998854.1
Mus musculus (house mouse)	Nucb1	NP_001157134.1
Rattus norvegicus (Norway rat)	Nucb1	NP_445915.1
Pan troglodytes (chimpanzee)	NUCB1	XP_512807.3
Bos taurus (cattle)	NUCB1	NP_001068667.1

Gene Ontology
Bibliography

Related articles in PubMed

Nucleobindin 1 (NUCB1) is a Golgi-resident marker of neurons.
Tulke S, Williams P, Hellysaz A, Ilegems E, Wendel M, Broberger C
Neuroscience314179-88(2016 Feb)

The novel neuropeptide phoenixin is highly co-expressed with nesfatin-1 in the rat hypothalamus, an immunohistochemical study.
Pa?asz A, Rojczyk E, Bogus K, Worthington JJ, Wiaderkiewicz R
Neuroscience letters59217-21(2015 Apr)

Modulation of food intake by mTOR signalling in the dorsal motor nucleus of the vagus in male rats: focus on ghrelin and nesfatin-1.
Zhang W, Zhang C, Fritze D, Chai B, Li J, Mulholland MW
Experimental physiology98(12)1696-704(2013 Dec)

G Protein binding sites on Calnuc (nucleobindin 1) and NUCB2 (nucleobindin 2) define a new class of G(alpha)i-regulatory motifs.
Garcia-Marcos M, Kietrsunthorn PS, Wang H, Ghosh P, Farquhar MG
The Journal of biological chemistry286(32)28138-49(2011 Aug)

Nucleobindin 1 is a calcium-regulated guanine nucleotide dissociation inhibitor of G{alpha}i1.
Kapoor N, Gupta R, Menon ST, Folta-Stogniew E, Raleigh DP, Sakmar TP
The Journal of biological chemistry285(41)31647-60(2010 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

NUCB1 mRNA and protein was found to be present in all brain regions, extending to the spinal cord and dorsal root ganglia; the protein was detected in cell somata and proximal dendrites, but not in axons or terminal structures. Further examination of the subcellular distribution of NUCB1 using organelle-specific markers revealed its consistent presence in the Golgi apparatus.
Title: Nucleobindin 1 (NUCB1) is a Golgi-resident marker of neurons.

a wide distribution of phoenixin in the hypothalamus which could implicate a potential functional relationship with nesfatin-1, possibly in the regulation of the hypothalamic-pituitary-gonadal axis or other autonomic functions
Title: The novel neuropeptide phoenixin is highly co-expressed with nesfatin-1 in the rat hypothalamus, an immunohistochemical study.

When administrated by injection into the fourth ventricle, nesfatin-1 suppressed food intake in comparison with the control vehicle.
Title: Modulation of food intake by mTOR signalling in the dorsal motor nucleus of the vagus in male rats: focus on ghrelin and nesfatin-1.

structural basis for the properties of Calnuc and NUCB2 binding to Galpha subunits and its regulation by calcium ions.
Title: G Protein binding sites on Calnuc (nucleobindin 1) and NUCB2 (nucleobindin 2) define a new class of G(alpha)i-regulatory motifs.

Nucleobindin 1 is a calcium-regulated guanine nucleotide dissociation inhibitor of G{alpha}i1.
Title: Nucleobindin 1 is a calcium-regulated guanine nucleotide dissociation inhibitor of G{alpha}i1.

The following Nucb1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nucb1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa09627	NM_053463.1 Latest version!	Rattus norvegicus nucleobindin 1 (Nucb1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00
ORa09627	XM_006229165.1 Latest version!	Rattus norvegicus nucleobindin 1 (Nucb1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00
ORa22790	XM_006229163.1 Latest version!	Rattus norvegicus nucleobindin 1 (Nucb1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
ORa22790	XM_006229164.3 Latest version!	Rattus norvegicus nucleobindin 1 (Nucb1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
ORa56881	XM_017589801.1 Latest version!	Rattus norvegicus nucleobindin 1 (Nucb1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
ORa56882	XM_017589802.1 Latest version!	Rattus norvegicus nucleobindin 1 (Nucb1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nucb1 ( NM_053463.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_445915.1 Rattus norvegicus nucleobindin 1 (Nucb1), mRNA.

Price & Availability↑

CloneID	ORa09627
Clone ID Related Accession (Same CDS sequence)	NM_053463.1 , XM_006229165.1
Accession Version	NM_053463.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1380bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-21
Organism	Rattus norvegicus(Norway rat)
Product	nucleobindin-1 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF336828.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF336828.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01906350 [ECO:0000348] ##Evidence-Data-END##

ATGCCTACCT CTGTGCCCCG CGGGGCGCCT TTCCTTCTCC TACCACCTTT ACTGATGCTG 
TCTGCTGTGC TGGCAGTGCC TGTGGACCGC GCAGCACCTC ATCAGGAGGA CAACCAGGCC 
ACTGAGACCC CGGACACAGG CCTGTACTAC CATCGGTACC TCCAGGAGGT CATCAACGTG 
CTAGAGACAG ATGGGCACTT CCGGGAGAAG CTGCAAGCTG CCAACGCTGA GGACATTAAG 
AGTGGAAAGC TGAGTCAAGA GCTGGACTTC GTCAGCCACA ACGTCCGCAC CAAGCTGGAT 
GAGCTCAAGC GACAGGAGGT ATCAAGGCTT CGGATGCTGC TCAAGGCCAA GATGGATGCA 
AAGCAGGAGC CTAACTTGCA GGTGGACCAC ATGAACCTTC TGAAGCAGTT TGAACACCTG 
GACCCTCAGA ACCAGCACAC GTTTGAGGCT CGGGACCTAG AGCTGCTGAT CCAGACGGCC 
ACCCGAGACC TCGCCCAGTA TGACGCTGCA CACCATGAAG AGTTCAAACG CTACGAGATG 
CTCAAGGAAC ATGAGCGAAG ACGTTACCTG GAGTCTCTGG GAGAGGAGCA GCGGAAGGAG 
GCTGAGAGGA AGCTACAAGA GCAGCAGCGC AGACACCGGG AACACCCCAA AGTCAATGTT 
CCTGGCAGCC AAGCCCAGTT GAAGGAGGTG TGGGAGGAGC TAGATGGATT GGACCCCAAC 
AGGTTCAACC CCAAGACCTT CTTCATACTG CATGACATCA ACAGCGATGG TGTCCTAGAT 
GAGCAAGAAC TGGAAGCTCT GTTTACCAAG GAGCTGGAGA AGGTGTACGA CCCGAAGAAC 
GAGGAGGACG ACATGAGGGA GATGGAGGAG GAGCGGCTGC GCATGCGGGA GCATGTGATG 
AAGAACGTGG ACACCAACCA GGACCGACTT GTGACCCTGG AGGAATTCCT GGCATCCACA 
CAGAGGAAGG AGTTTGGGGA AACTGCGGAG GGATGGAAGA CAGTGGAAAT GTATCCAGCC 
TACACAGAGG AAGAGCTGAA GCGTTTTGAA GAGGAGCTTG CTGCCCGGGA GGCTGAGCTA 
AATGCCAGAG CCCAGCGCCT CAGCCAGGAG ACAGAGGCCC TGGGGCGCTC CCAGGACCGC 
CTAGAGGCTC AGAAGAGAGA GCTGCAGCAG GCCGTTCTGC AGATGGAGCA AAGGAAGCAG 
CAACAGCAAG AGCAGAGCGC TCCGCCTTCC CAACCTGATG GGCAGCTGCA GTTCCGTGCA 
GACACAGGGG ATGCTCCTGT CCCAGCTCCA GCAGGTGACC AGAAAGACGT GCCTGCTTCT 
GAAAAGAAGG TTCCAGAGCA GCCCCCTGTG CTGCCACAGC TGGATTCTCA GCATTTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_445915.1
CDS	3..1382
Translation	MPTSVPRGAPFLLLPPLLMLSAVLAVPVDRAAPHQEDNQATETPDTGLYYHRYLQEVINVLETDGHFREKLQAANAEDIKSGKLSQELDFVSHNVRTKLDELKRQEVSRLRMLLKAKMDAKQEPNLQVDHMNLLKQFEHLDPQNQHTFEARDLELLIQTATRDLAQYDAAHHEEFKRYEMLKEHERRRYLESLGEEQRKEAERKLQEQQRRHREHPKVNVPGSQAQLKEVWEELDGLDPNRFNPKTFFILHDINSDGVLDEQELEALFTKELEKVYDPKNEEDDMREMEEERLRMREHVMKNVDTNQDRLVTLEEFLASTQRKEFGETAEGWKTVEMYPAYTEEELKRFEEELAAREAELNARAQRLSQETEALGRSQDRLEAQKRELQQAVLQMEQRKQQQQEQSAPPSQPDGQLQFRADTGDAPVPAPAGDQKDVPASEKKVPEQPPVLPQLDSQHL

Target ORF information:

RefSeq Version	NM_053463.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus nucleobindin 1 (Nucb1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_053463.1

ORF Insert Sequence:

ATGCCTACCT CTGTGCCCCG CGGGGCGCCT TTCCTTCTCC TACCACCTTT ACTGATGCTG 
TCTGCTGTGC TGGCAGTGCC TGTGGACCGC GCAGCACCTC ATCAGGAGGA CAACCAGGCC 
ACTGAGACCC CGGACACAGG CCTGTACTAC CATCGGTACC TCCAGGAGGT CATCAACGTG 
CTAGAGACAG ATGGGCACTT CCGGGAGAAG CTGCAAGCTG CCAACGCTGA GGACATTAAG 
AGTGGAAAGC TGAGTCAAGA GCTGGACTTC GTCAGCCACA ACGTCCGCAC CAAGCTGGAT 
GAGCTCAAGC GACAGGAGGT ATCAAGGCTT CGGATGCTGC TCAAGGCCAA GATGGATGCA 
AAGCAGGAGC CTAACTTGCA GGTGGACCAC ATGAACCTTC TGAAGCAGTT TGAACACCTG 
GACCCTCAGA ACCAGCACAC GTTTGAGGCT CGGGACCTAG AGCTGCTGAT CCAGACGGCC 
ACCCGAGACC TCGCCCAGTA TGACGCTGCA CACCATGAAG AGTTCAAACG CTACGAGATG 
CTCAAGGAAC ATGAGCGAAG ACGTTACCTG GAGTCTCTGG GAGAGGAGCA GCGGAAGGAG 
GCTGAGAGGA AGCTACAAGA GCAGCAGCGC AGACACCGGG AACACCCCAA AGTCAATGTT 
CCTGGCAGCC AAGCCCAGTT GAAGGAGGTG TGGGAGGAGC TAGATGGATT GGACCCCAAC 
AGGTTCAACC CCAAGACCTT CTTCATACTG CATGACATCA ACAGCGATGG TGTCCTAGAT 
GAGCAAGAAC TGGAAGCTCT GTTTACCAAG GAGCTGGAGA AGGTGTACGA CCCGAAGAAC 
GAGGAGGACG ACATGAGGGA GATGGAGGAG GAGCGGCTGC GCATGCGGGA GCATGTGATG 
AAGAACGTGG ACACCAACCA GGACCGACTT GTGACCCTGG AGGAATTCCT GGCATCCACA 
CAGAGGAAGG AGTTTGGGGA AACTGCGGAG GGATGGAAGA CAGTGGAAAT GTATCCAGCC 
TACACAGAGG AAGAGCTGAA GCGTTTTGAA GAGGAGCTTG CTGCCCGGGA GGCTGAGCTA 
AATGCCAGAG CCCAGCGCCT CAGCCAGGAG ACAGAGGCCC TGGGGCGCTC CCAGGACCGC 
CTAGAGGCTC AGAAGAGAGA GCTGCAGCAG GCCGTTCTGC AGATGGAGCA AAGGAAGCAG 
CAACAGCAAG AGCAGAGCGC TCCGCCTTCC CAACCTGATG GGCAGCTGCA GTTCCGTGCA 
GACACAGGGG ATGCTCCTGT CCCAGCTCCA GCAGGTGACC AGAAAGACGT GCCTGCTTCT 
GAAAAGAAGG TTCCAGAGCA GCCCCCTGTG CTGCCACAGC TGGATTCTCA GCATTTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nucb1 ( XM_006229165.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006229227.1 Rattus norvegicus nucleobindin 1 (Nucb1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	ORa09627
Clone ID Related Accession (Same CDS sequence)	NM_053463.1 , XM_006229165.1
Accession Version	XM_006229165.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1380bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	nucleobindin-1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTACCT CTGTGCCCCG CGGGGCGCCT TTCCTTCTCC TACCACCTTT ACTGATGCTG 
TCTGCTGTGC TGGCAGTGCC TGTGGACCGC GCAGCACCTC ATCAGGAGGA CAACCAGGCC 
ACTGAGACCC CGGACACAGG CCTGTACTAC CATCGGTACC TCCAGGAGGT CATCAACGTG 
CTAGAGACAG ATGGGCACTT CCGGGAGAAG CTGCAAGCTG CCAACGCTGA GGACATTAAG 
AGTGGAAAGC TGAGTCAAGA GCTGGACTTC GTCAGCCACA ACGTCCGCAC CAAGCTGGAT 
GAGCTCAAGC GACAGGAGGT ATCAAGGCTT CGGATGCTGC TCAAGGCCAA GATGGATGCA 
AAGCAGGAGC CTAACTTGCA GGTGGACCAC ATGAACCTTC TGAAGCAGTT TGAACACCTG 
GACCCTCAGA ACCAGCACAC GTTTGAGGCT CGGGACCTAG AGCTGCTGAT CCAGACGGCC 
ACCCGAGACC TCGCCCAGTA TGACGCTGCA CACCATGAAG AGTTCAAACG CTACGAGATG 
CTCAAGGAAC ATGAGCGAAG ACGTTACCTG GAGTCTCTGG GAGAGGAGCA GCGGAAGGAG 
GCTGAGAGGA AGCTACAAGA GCAGCAGCGC AGACACCGGG AACACCCCAA AGTCAATGTT 
CCTGGCAGCC AAGCCCAGTT GAAGGAGGTG TGGGAGGAGC TAGATGGATT GGACCCCAAC 
AGGTTCAACC CCAAGACCTT CTTCATACTG CATGACATCA ACAGCGATGG TGTCCTAGAT 
GAGCAAGAAC TGGAAGCTCT GTTTACCAAG GAGCTGGAGA AGGTGTACGA CCCGAAGAAC 
GAGGAGGACG ACATGAGGGA GATGGAGGAG GAGCGGCTGC GCATGCGGGA GCATGTGATG 
AAGAACGTGG ACACCAACCA GGACCGACTT GTGACCCTGG AGGAATTCCT GGCATCCACA 
CAGAGGAAGG AGTTTGGGGA AACTGCGGAG GGATGGAAGA CAGTGGAAAT GTATCCAGCC 
TACACAGAGG AAGAGCTGAA GCGTTTTGAA GAGGAGCTTG CTGCCCGGGA GGCTGAGCTA 
AATGCCAGAG CCCAGCGCCT CAGCCAGGAG ACAGAGGCCC TGGGGCGCTC CCAGGACCGC 
CTAGAGGCTC AGAAGAGAGA GCTGCAGCAG GCCGTTCTGC AGATGGAGCA AAGGAAGCAG 
CAACAGCAAG AGCAGAGCGC TCCGCCTTCC CAACCTGATG GGCAGCTGCA GTTCCGTGCA 
GACACAGGGG ATGCTCCTGT CCCAGCTCCA GCAGGTGACC AGAAAGACGT GCCTGCTTCT 
GAAAAGAAGG TTCCAGAGCA GCCCCCTGTG CTGCCACAGC TGGATTCTCA GCATTTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006229227.1
CDS	120..1499
Translation	MPTSVPRGAPFLLLPPLLMLSAVLAVPVDRAAPHQEDNQATETPDTGLYYHRYLQEVINVLETDGHFREKLQAANAEDIKSGKLSQELDFVSHNVRTKLDELKRQEVSRLRMLLKAKMDAKQEPNLQVDHMNLLKQFEHLDPQNQHTFEARDLELLIQTATRDLAQYDAAHHEEFKRYEMLKEHERRRYLESLGEEQRKEAERKLQEQQRRHREHPKVNVPGSQAQLKEVWEELDGLDPNRFNPKTFFILHDINSDGVLDEQELEALFTKELEKVYDPKNEEDDMREMEEERLRMREHVMKNVDTNQDRLVTLEEFLASTQRKEFGETAEGWKTVEMYPAYTEEELKRFEEELAAREAELNARAQRLSQETEALGRSQDRLEAQKRELQQAVLQMEQRKQQQQEQSAPPSQPDGQLQFRADTGDAPVPAPAGDQKDVPASEKKVPEQPPVLPQLDSQHL

Target ORF information:

RefSeq Version	XM_006229165.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus nucleobindin 1 (Nucb1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006229165.1

ORF Insert Sequence:

ATGCCTACCT CTGTGCCCCG CGGGGCGCCT TTCCTTCTCC TACCACCTTT ACTGATGCTG 
TCTGCTGTGC TGGCAGTGCC TGTGGACCGC GCAGCACCTC ATCAGGAGGA CAACCAGGCC 
ACTGAGACCC CGGACACAGG CCTGTACTAC CATCGGTACC TCCAGGAGGT CATCAACGTG 
CTAGAGACAG ATGGGCACTT CCGGGAGAAG CTGCAAGCTG CCAACGCTGA GGACATTAAG 
AGTGGAAAGC TGAGTCAAGA GCTGGACTTC GTCAGCCACA ACGTCCGCAC CAAGCTGGAT 
GAGCTCAAGC GACAGGAGGT ATCAAGGCTT CGGATGCTGC TCAAGGCCAA GATGGATGCA 
AAGCAGGAGC CTAACTTGCA GGTGGACCAC ATGAACCTTC TGAAGCAGTT TGAACACCTG 
GACCCTCAGA ACCAGCACAC GTTTGAGGCT CGGGACCTAG AGCTGCTGAT CCAGACGGCC 
ACCCGAGACC TCGCCCAGTA TGACGCTGCA CACCATGAAG AGTTCAAACG CTACGAGATG 
CTCAAGGAAC ATGAGCGAAG ACGTTACCTG GAGTCTCTGG GAGAGGAGCA GCGGAAGGAG 
GCTGAGAGGA AGCTACAAGA GCAGCAGCGC AGACACCGGG AACACCCCAA AGTCAATGTT 
CCTGGCAGCC AAGCCCAGTT GAAGGAGGTG TGGGAGGAGC TAGATGGATT GGACCCCAAC 
AGGTTCAACC CCAAGACCTT CTTCATACTG CATGACATCA ACAGCGATGG TGTCCTAGAT 
GAGCAAGAAC TGGAAGCTCT GTTTACCAAG GAGCTGGAGA AGGTGTACGA CCCGAAGAAC 
GAGGAGGACG ACATGAGGGA GATGGAGGAG GAGCGGCTGC GCATGCGGGA GCATGTGATG 
AAGAACGTGG ACACCAACCA GGACCGACTT GTGACCCTGG AGGAATTCCT GGCATCCACA 
CAGAGGAAGG AGTTTGGGGA AACTGCGGAG GGATGGAAGA CAGTGGAAAT GTATCCAGCC 
TACACAGAGG AAGAGCTGAA GCGTTTTGAA GAGGAGCTTG CTGCCCGGGA GGCTGAGCTA 
AATGCCAGAG CCCAGCGCCT CAGCCAGGAG ACAGAGGCCC TGGGGCGCTC CCAGGACCGC 
CTAGAGGCTC AGAAGAGAGA GCTGCAGCAG GCCGTTCTGC AGATGGAGCA AAGGAAGCAG 
CAACAGCAAG AGCAGAGCGC TCCGCCTTCC CAACCTGATG GGCAGCTGCA GTTCCGTGCA 
GACACAGGGG ATGCTCCTGT CCCAGCTCCA GCAGGTGACC AGAAAGACGT GCCTGCTTCT 
GAAAAGAAGG TTCCAGAGCA GCCCCCTGTG CTGCCACAGC TGGATTCTCA GCATTTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nucb1 ( XM_006229163.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006229225.1 Rattus norvegicus nucleobindin 1 (Nucb1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa22790
Clone ID Related Accession (Same CDS sequence)	XM_006229163.1 , XM_006229164.3
Accession Version	XM_006229163.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1383bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	nucleobindin-1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTACCT CTGTGCCCCG CGGGGCGCCT TTCCTTCTCC TACCACCTTT ACTGATGCTG 
TCTGCTGTGC TGGCAGTGCC TGTGGACCGC GCAGCACCTC ATCAGGAGGA CAACCAGGCC 
ACTGAGACCC CGGACACAGG CCTGTACTAC CATCGGTACC TCCAGGAGGT CATCAACGTG 
CTAGAGACAG ATGGGCACTT CCGGGAGAAG CTGCAAGCTG CCAACGCTGA GGACATTAAG 
AGTGGAAAGC TGAGTCAAGA GCTGGACTTC GTCAGCCACA ACGTCCGCAC CAAGCTGGAT 
GAGCTCAAGC GACAGGAGGT ATCAAGGCTT CGGATGCTGC TCAAGGCCAA GATGGATGCA 
AAGCAGGAGC CTACAGACTT GCAGGTGGAC CACATGAACC TTCTGAAGCA GTTTGAACAC 
CTGGACCCTC AGAACCAGCA CACGTTTGAG GCTCGGGACC TAGAGCTGCT GATCCAGACG 
GCCACCCGAG ACCTCGCCCA GTATGACGCT GCACACCATG AAGAGTTCAA ACGCTACGAG 
ATGCTCAAGG AACATGAGCG AAGACGTTAC CTGGAGTCTC TGGGAGAGGA GCAGCGGAAG 
GAGGCTGAGA GGAAGCTACA AGAGCAGCAG CGCAGACACC GGGAACACCC CAAAGTCAAT 
GTTCCTGGCA GCCAAGCCCA GTTGAAGGAG GTGTGGGAGG AGCTAGATGG ATTGGACCCC 
AACAGGTTCA ACCCCAAGAC CTTCTTCATA CTGCATGACA TCAACAGCGA TGGTGTCCTA 
GATGAGCAAG AACTGGAAGC TCTGTTTACC AAGGAGCTGG AGAAGGTGTA CGACCCGAAG 
AACGAGGAGG ACGACATGAG GGAGATGGAG GAGGAGCGGC TGCGCATGCG GGAGCATGTG 
ATGAAGAACG TGGACACCAA CCAGGACCGA CTTGTGACCC TGGAGGAATT CCTGGCATCC 
ACACAGAGGA AGGAGTTTGG GGAAACTGCG GAGGGATGGA AGACAGTGGA AATGTATCCA 
GCCTACACAG AGGAAGAGCT GAAGCGTTTT GAAGAGGAGC TTGCTGCCCG GGAGGCTGAG 
CTAAATGCCA GAGCCCAGCG CCTCAGCCAG GAGACAGAGG CCCTGGGGCG CTCCCAGGAC 
CGCCTAGAGG CTCAGAAGAG AGAGCTGCAG CAGGCCGTTC TGCAGATGGA GCAAAGGAAG 
CAGCAACAGC AAGAGCAGAG CGCTCCGCCT TCCCAACCTG ATGGGCAGCT GCAGTTCCGT 
GCAGACACAG GGGATGCTCC TGTCCCAGCT CCAGCAGGTG ACCAGAAAGA CGTGCCTGCT 
TCTGAAAAGA AGGTTCCAGA GCAGCCCCCT GTGCTGCCAC AGCTGGATTC TCAGCATTTA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006229225.1
CDS	120..1502
Translation	MPTSVPRGAPFLLLPPLLMLSAVLAVPVDRAAPHQEDNQATETPDTGLYYHRYLQEVINVLETDGHFREKLQAANAEDIKSGKLSQELDFVSHNVRTKLDELKRQEVSRLRMLLKAKMDAKQEPTDLQVDHMNLLKQFEHLDPQNQHTFEARDLELLIQTATRDLAQYDAAHHEEFKRYEMLKEHERRRYLESLGEEQRKEAERKLQEQQRRHREHPKVNVPGSQAQLKEVWEELDGLDPNRFNPKTFFILHDINSDGVLDEQELEALFTKELEKVYDPKNEEDDMREMEEERLRMREHVMKNVDTNQDRLVTLEEFLASTQRKEFGETAEGWKTVEMYPAYTEEELKRFEEELAAREAELNARAQRLSQETEALGRSQDRLEAQKRELQQAVLQMEQRKQQQQEQSAPPSQPDGQLQFRADTGDAPVPAPAGDQKDVPASEKKVPEQPPVLPQLDSQHL

Target ORF information:

RefSeq Version	XM_006229163.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus nucleobindin 1 (Nucb1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006229163.1

ORF Insert Sequence:

ATGCCTACCT CTGTGCCCCG CGGGGCGCCT TTCCTTCTCC TACCACCTTT ACTGATGCTG 
TCTGCTGTGC TGGCAGTGCC TGTGGACCGC GCAGCACCTC ATCAGGAGGA CAACCAGGCC 
ACTGAGACCC CGGACACAGG CCTGTACTAC CATCGGTACC TCCAGGAGGT CATCAACGTG 
CTAGAGACAG ATGGGCACTT CCGGGAGAAG CTGCAAGCTG CCAACGCTGA GGACATTAAG 
AGTGGAAAGC TGAGTCAAGA GCTGGACTTC GTCAGCCACA ACGTCCGCAC CAAGCTGGAT 
GAGCTCAAGC GACAGGAGGT ATCAAGGCTT CGGATGCTGC TCAAGGCCAA GATGGATGCA 
AAGCAGGAGC CTACAGACTT GCAGGTGGAC CACATGAACC TTCTGAAGCA GTTTGAACAC 
CTGGACCCTC AGAACCAGCA CACGTTTGAG GCTCGGGACC TAGAGCTGCT GATCCAGACG 
GCCACCCGAG ACCTCGCCCA GTATGACGCT GCACACCATG AAGAGTTCAA ACGCTACGAG 
ATGCTCAAGG AACATGAGCG AAGACGTTAC CTGGAGTCTC TGGGAGAGGA GCAGCGGAAG 
GAGGCTGAGA GGAAGCTACA AGAGCAGCAG CGCAGACACC GGGAACACCC CAAAGTCAAT 
GTTCCTGGCA GCCAAGCCCA GTTGAAGGAG GTGTGGGAGG AGCTAGATGG ATTGGACCCC 
AACAGGTTCA ACCCCAAGAC CTTCTTCATA CTGCATGACA TCAACAGCGA TGGTGTCCTA 
GATGAGCAAG AACTGGAAGC TCTGTTTACC AAGGAGCTGG AGAAGGTGTA CGACCCGAAG 
AACGAGGAGG ACGACATGAG GGAGATGGAG GAGGAGCGGC TGCGCATGCG GGAGCATGTG 
ATGAAGAACG TGGACACCAA CCAGGACCGA CTTGTGACCC TGGAGGAATT CCTGGCATCC 
ACACAGAGGA AGGAGTTTGG GGAAACTGCG GAGGGATGGA AGACAGTGGA AATGTATCCA 
GCCTACACAG AGGAAGAGCT GAAGCGTTTT GAAGAGGAGC TTGCTGCCCG GGAGGCTGAG 
CTAAATGCCA GAGCCCAGCG CCTCAGCCAG GAGACAGAGG CCCTGGGGCG CTCCCAGGAC 
CGCCTAGAGG CTCAGAAGAG AGAGCTGCAG CAGGCCGTTC TGCAGATGGA GCAAAGGAAG 
CAGCAACAGC AAGAGCAGAG CGCTCCGCCT TCCCAACCTG ATGGGCAGCT GCAGTTCCGT 
GCAGACACAG GGGATGCTCC TGTCCCAGCT CCAGCAGGTG ACCAGAAAGA CGTGCCTGCT 
TCTGAAAAGA AGGTTCCAGA GCAGCCCCCT GTGCTGCCAC AGCTGGATTC TCAGCATTTA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nucb1 ( XM_006229164.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006229226.1 Rattus norvegicus nucleobindin 1 (Nucb1), transcript variant X4, mRNA.

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CloneID	ORa22790
Clone ID Related Accession (Same CDS sequence)	XM_006229163.1 , XM_006229164.3
Accession Version	XM_006229164.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1383bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	nucleobindin-1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006229164.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTACCT CTGTGCCCCG CGGGGCGCCT TTCCTTCTCC TACCACCTTT ACTGATGCTG 
TCTGCTGTGC TGGCAGTGCC TGTGGACCGC GCAGCACCTC ATCAGGAGGA CAACCAGGCC 
ACTGAGACCC CGGACACAGG CCTGTACTAC CATCGGTACC TCCAGGAGGT CATCAACGTG 
CTAGAGACAG ATGGGCACTT CCGGGAGAAG CTGCAAGCTG CCAACGCTGA GGACATTAAG 
AGTGGAAAGC TGAGTCAAGA GCTGGACTTC GTCAGCCACA ACGTCCGCAC CAAGCTGGAT 
GAGCTCAAGC GACAGGAGGT ATCAAGGCTT CGGATGCTGC TCAAGGCCAA GATGGATGCA 
AAGCAGGAGC CTACAGACTT GCAGGTGGAC CACATGAACC TTCTGAAGCA GTTTGAACAC 
CTGGACCCTC AGAACCAGCA CACGTTTGAG GCTCGGGACC TAGAGCTGCT GATCCAGACG 
GCCACCCGAG ACCTCGCCCA GTATGACGCT GCACACCATG AAGAGTTCAA ACGCTACGAG 
ATGCTCAAGG AACATGAGCG AAGACGTTAC CTGGAGTCTC TGGGAGAGGA GCAGCGGAAG 
GAGGCTGAGA GGAAGCTACA AGAGCAGCAG CGCAGACACC GGGAACACCC CAAAGTCAAT 
GTTCCTGGCA GCCAAGCCCA GTTGAAGGAG GTGTGGGAGG AGCTAGATGG ATTGGACCCC 
AACAGGTTCA ACCCCAAGAC CTTCTTCATA CTGCATGACA TCAACAGCGA TGGTGTCCTA 
GATGAGCAAG AACTGGAAGC TCTGTTTACC AAGGAGCTGG AGAAGGTGTA CGACCCGAAG 
AACGAGGAGG ACGACATGAG GGAGATGGAG GAGGAGCGGC TGCGCATGCG GGAGCATGTG 
ATGAAGAACG TGGACACCAA CCAGGACCGA CTTGTGACCC TGGAGGAATT CCTGGCATCC 
ACACAGAGGA AGGAGTTTGG GGAAACTGCG GAGGGATGGA AGACAGTGGA AATGTATCCA 
GCCTACACAG AGGAAGAGCT GAAGCGTTTT GAAGAGGAGC TTGCTGCCCG GGAGGCTGAG 
CTAAATGCCA GAGCCCAGCG CCTCAGCCAG GAGACAGAGG CCCTGGGGCG CTCCCAGGAC 
CGCCTAGAGG CTCAGAAGAG AGAGCTGCAG CAGGCCGTTC TGCAGATGGA GCAAAGGAAG 
CAGCAACAGC AAGAGCAGAG CGCTCCGCCT TCCCAACCTG ATGGGCAGCT GCAGTTCCGT 
GCAGACACAG GGGATGCTCC TGTCCCAGCT CCAGCAGGTG ACCAGAAAGA CGTGCCTGCT 
TCTGAAAAGA AGGTTCCAGA GCAGCCCCCT GTGCTGCCAC AGCTGGATTC TCAGCATTTA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006229226.1
CDS	81..1463
Translation	MPTSVPRGAPFLLLPPLLMLSAVLAVPVDRAAPHQEDNQATETPDTGLYYHRYLQEVINVLETDGHFREKLQAANAEDIKSGKLSQELDFVSHNVRTKLDELKRQEVSRLRMLLKAKMDAKQEPTDLQVDHMNLLKQFEHLDPQNQHTFEARDLELLIQTATRDLAQYDAAHHEEFKRYEMLKEHERRRYLESLGEEQRKEAERKLQEQQRRHREHPKVNVPGSQAQLKEVWEELDGLDPNRFNPKTFFILHDINSDGVLDEQELEALFTKELEKVYDPKNEEDDMREMEEERLRMREHVMKNVDTNQDRLVTLEEFLASTQRKEFGETAEGWKTVEMYPAYTEEELKRFEEELAAREAELNARAQRLSQETEALGRSQDRLEAQKRELQQAVLQMEQRKQQQQEQSAPPSQPDGQLQFRADTGDAPVPAPAGDQKDVPASEKKVPEQPPVLPQLDSQHL

Target ORF information:

RefSeq Version	XM_006229164.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus nucleobindin 1 (Nucb1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006229164.3

ORF Insert Sequence:

ATGCCTACCT CTGTGCCCCG CGGGGCGCCT TTCCTTCTCC TACCACCTTT ACTGATGCTG 
TCTGCTGTGC TGGCAGTGCC TGTGGACCGC GCAGCACCTC ATCAGGAGGA CAACCAGGCC 
ACTGAGACCC CGGACACAGG CCTGTACTAC CATCGGTACC TCCAGGAGGT CATCAACGTG 
CTAGAGACAG ATGGGCACTT CCGGGAGAAG CTGCAAGCTG CCAACGCTGA GGACATTAAG 
AGTGGAAAGC TGAGTCAAGA GCTGGACTTC GTCAGCCACA ACGTCCGCAC CAAGCTGGAT 
GAGCTCAAGC GACAGGAGGT ATCAAGGCTT CGGATGCTGC TCAAGGCCAA GATGGATGCA 
AAGCAGGAGC CTACAGACTT GCAGGTGGAC CACATGAACC TTCTGAAGCA GTTTGAACAC 
CTGGACCCTC AGAACCAGCA CACGTTTGAG GCTCGGGACC TAGAGCTGCT GATCCAGACG 
GCCACCCGAG ACCTCGCCCA GTATGACGCT GCACACCATG AAGAGTTCAA ACGCTACGAG 
ATGCTCAAGG AACATGAGCG AAGACGTTAC CTGGAGTCTC TGGGAGAGGA GCAGCGGAAG 
GAGGCTGAGA GGAAGCTACA AGAGCAGCAG CGCAGACACC GGGAACACCC CAAAGTCAAT 
GTTCCTGGCA GCCAAGCCCA GTTGAAGGAG GTGTGGGAGG AGCTAGATGG ATTGGACCCC 
AACAGGTTCA ACCCCAAGAC CTTCTTCATA CTGCATGACA TCAACAGCGA TGGTGTCCTA 
GATGAGCAAG AACTGGAAGC TCTGTTTACC AAGGAGCTGG AGAAGGTGTA CGACCCGAAG 
AACGAGGAGG ACGACATGAG GGAGATGGAG GAGGAGCGGC TGCGCATGCG GGAGCATGTG 
ATGAAGAACG TGGACACCAA CCAGGACCGA CTTGTGACCC TGGAGGAATT CCTGGCATCC 
ACACAGAGGA AGGAGTTTGG GGAAACTGCG GAGGGATGGA AGACAGTGGA AATGTATCCA 
GCCTACACAG AGGAAGAGCT GAAGCGTTTT GAAGAGGAGC TTGCTGCCCG GGAGGCTGAG 
CTAAATGCCA GAGCCCAGCG CCTCAGCCAG GAGACAGAGG CCCTGGGGCG CTCCCAGGAC 
CGCCTAGAGG CTCAGAAGAG AGAGCTGCAG CAGGCCGTTC TGCAGATGGA GCAAAGGAAG 
CAGCAACAGC AAGAGCAGAG CGCTCCGCCT TCCCAACCTG ATGGGCAGCT GCAGTTCCGT 
GCAGACACAG GGGATGCTCC TGTCCCAGCT CCAGCAGGTG ACCAGAAAGA CGTGCCTGCT 
TCTGAAAAGA AGGTTCCAGA GCAGCCCCCT GTGCTGCCAC AGCTGGATTC TCAGCATTTA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nucb1 ( XM_017589801.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017445290.1 Rattus norvegicus nucleobindin 1 (Nucb1), transcript variant X1, mRNA.

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CloneID	ORa56881
Clone ID Related Accession (Same CDS sequence)	XM_017589801.1
Accession Version	XM_017589801.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1404bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	nucleobindin-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAATTG AACCAGCTAC GATGCCTACC TCTGTGCCCC GCGGGGCGCC TTTCCTTCTC 
CTACCACCTT TACTGATGCT GTCTGCTGTG CTGGCAGTGC CTGTGGACCG CGCAGCACCT 
CATCAGGAGG ACAACCAGGC CACTGAGACC CCGGACACAG GCCTGTACTA CCATCGGTAC 
CTCCAGGAGG TCATCAACGT GCTAGAGACA GATGGGCACT TCCGGGAGAA GCTGCAAGCT 
GCCAACGCTG AGGACATTAA GAGTGGAAAG CTGAGTCAAG AGCTGGACTT CGTCAGCCAC 
AACGTCCGCA CCAAGCTGGA TGAGCTCAAG CGACAGGAGG TATCAAGGCT TCGGATGCTG 
CTCAAGGCCA AGATGGATGC AAAGCAGGAG CCTACAGACT TGCAGGTGGA CCACATGAAC 
CTTCTGAAGC AGTTTGAACA CCTGGACCCT CAGAACCAGC ACACGTTTGA GGCTCGGGAC 
CTAGAGCTGC TGATCCAGAC GGCCACCCGA GACCTCGCCC AGTATGACGC TGCACACCAT 
GAAGAGTTCA AACGCTACGA GATGCTCAAG GAACATGAGC GAAGACGTTA CCTGGAGTCT 
CTGGGAGAGG AGCAGCGGAA GGAGGCTGAG AGGAAGCTAC AAGAGCAGCA GCGCAGACAC 
CGGGAACACC CCAAAGTCAA TGTTCCTGGC AGCCAAGCCC AGTTGAAGGA GGTGTGGGAG 
GAGCTAGATG GATTGGACCC CAACAGGTTC AACCCCAAGA CCTTCTTCAT ACTGCATGAC 
ATCAACAGCG ATGGTGTCCT AGATGAGCAA GAACTGGAAG CTCTGTTTAC CAAGGAGCTG 
GAGAAGGTGT ACGACCCGAA GAACGAGGAG GACGACATGA GGGAGATGGA GGAGGAGCGG 
CTGCGCATGC GGGAGCATGT GATGAAGAAC GTGGACACCA ACCAGGACCG ACTTGTGACC 
CTGGAGGAAT TCCTGGCATC CACACAGAGG AAGGAGTTTG GGGAAACTGC GGAGGGATGG 
AAGACAGTGG AAATGTATCC AGCCTACACA GAGGAAGAGC TGAAGCGTTT TGAAGAGGAG 
CTTGCTGCCC GGGAGGCTGA GCTAAATGCC AGAGCCCAGC GCCTCAGCCA GGAGACAGAG 
GCCCTGGGGC GCTCCCAGGA CCGCCTAGAG GCTCAGAAGA GAGAGCTGCA GCAGGCCGTT 
CTGCAGATGG AGCAAAGGAA GCAGCAACAG CAAGAGCAGA GCGCTCCGCC TTCCCAACCT 
GATGGGCAGC TGCAGTTCCG TGCAGACACA GGGGATGCTC CTGTCCCAGC TCCAGCAGGT 
GACCAGAAAG ACGTGCCTGC TTCTGAAAAG AAGGTTCCAG AGCAGCCCCC TGTGCTGCCA 
CAGCTGGATT CTCAGCATTT ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017445290.1
CDS	14..1417
Translation	MKIEPATMPTSVPRGAPFLLLPPLLMLSAVLAVPVDRAAPHQEDNQATETPDTGLYYHRYLQEVINVLETDGHFREKLQAANAEDIKSGKLSQELDFVSHNVRTKLDELKRQEVSRLRMLLKAKMDAKQEPTDLQVDHMNLLKQFEHLDPQNQHTFEARDLELLIQTATRDLAQYDAAHHEEFKRYEMLKEHERRRYLESLGEEQRKEAERKLQEQQRRHREHPKVNVPGSQAQLKEVWEELDGLDPNRFNPKTFFILHDINSDGVLDEQELEALFTKELEKVYDPKNEEDDMREMEEERLRMREHVMKNVDTNQDRLVTLEEFLASTQRKEFGETAEGWKTVEMYPAYTEEELKRFEEELAAREAELNARAQRLSQETEALGRSQDRLEAQKRELQQAVLQMEQRKQQQQEQSAPPSQPDGQLQFRADTGDAPVPAPAGDQKDVPASEKKVPEQPPVLPQLDSQHL

Target ORF information:

RefSeq Version	XM_017589801.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus nucleobindin 1 (Nucb1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017589801.1

ORF Insert Sequence:

ATGAAAATTG AACCAGCTAC GATGCCTACC TCTGTGCCCC GCGGGGCGCC TTTCCTTCTC 
CTACCACCTT TACTGATGCT GTCTGCTGTG CTGGCAGTGC CTGTGGACCG CGCAGCACCT 
CATCAGGAGG ACAACCAGGC CACTGAGACC CCGGACACAG GCCTGTACTA CCATCGGTAC 
CTCCAGGAGG TCATCAACGT GCTAGAGACA GATGGGCACT TCCGGGAGAA GCTGCAAGCT 
GCCAACGCTG AGGACATTAA GAGTGGAAAG CTGAGTCAAG AGCTGGACTT CGTCAGCCAC 
AACGTCCGCA CCAAGCTGGA TGAGCTCAAG CGACAGGAGG TATCAAGGCT TCGGATGCTG 
CTCAAGGCCA AGATGGATGC AAAGCAGGAG CCTACAGACT TGCAGGTGGA CCACATGAAC 
CTTCTGAAGC AGTTTGAACA CCTGGACCCT CAGAACCAGC ACACGTTTGA GGCTCGGGAC 
CTAGAGCTGC TGATCCAGAC GGCCACCCGA GACCTCGCCC AGTATGACGC TGCACACCAT 
GAAGAGTTCA AACGCTACGA GATGCTCAAG GAACATGAGC GAAGACGTTA CCTGGAGTCT 
CTGGGAGAGG AGCAGCGGAA GGAGGCTGAG AGGAAGCTAC AAGAGCAGCA GCGCAGACAC 
CGGGAACACC CCAAAGTCAA TGTTCCTGGC AGCCAAGCCC AGTTGAAGGA GGTGTGGGAG 
GAGCTAGATG GATTGGACCC CAACAGGTTC AACCCCAAGA CCTTCTTCAT ACTGCATGAC 
ATCAACAGCG ATGGTGTCCT AGATGAGCAA GAACTGGAAG CTCTGTTTAC CAAGGAGCTG 
GAGAAGGTGT ACGACCCGAA GAACGAGGAG GACGACATGA GGGAGATGGA GGAGGAGCGG 
CTGCGCATGC GGGAGCATGT GATGAAGAAC GTGGACACCA ACCAGGACCG ACTTGTGACC 
CTGGAGGAAT TCCTGGCATC CACACAGAGG AAGGAGTTTG GGGAAACTGC GGAGGGATGG 
AAGACAGTGG AAATGTATCC AGCCTACACA GAGGAAGAGC TGAAGCGTTT TGAAGAGGAG 
CTTGCTGCCC GGGAGGCTGA GCTAAATGCC AGAGCCCAGC GCCTCAGCCA GGAGACAGAG 
GCCCTGGGGC GCTCCCAGGA CCGCCTAGAG GCTCAGAAGA GAGAGCTGCA GCAGGCCGTT 
CTGCAGATGG AGCAAAGGAA GCAGCAACAG CAAGAGCAGA GCGCTCCGCC TTCCCAACCT 
GATGGGCAGC TGCAGTTCCG TGCAGACACA GGGGATGCTC CTGTCCCAGC TCCAGCAGGT 
GACCAGAAAG ACGTGCCTGC TTCTGAAAAG AAGGTTCCAG AGCAGCCCCC TGTGCTGCCA 
CAGCTGGATT CTCAGCATTT ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nucb1 ( XM_017589802.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017445291.1 Rattus norvegicus nucleobindin 1 (Nucb1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa56882
Clone ID Related Accession (Same CDS sequence)	XM_017589802.1
Accession Version	XM_017589802.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1401bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	nucleobindin-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAATTG AACCAGCTAC GATGCCTACC TCTGTGCCCC GCGGGGCGCC TTTCCTTCTC 
CTACCACCTT TACTGATGCT GTCTGCTGTG CTGGCAGTGC CTGTGGACCG CGCAGCACCT 
CATCAGGAGG ACAACCAGGC CACTGAGACC CCGGACACAG GCCTGTACTA CCATCGGTAC 
CTCCAGGAGG TCATCAACGT GCTAGAGACA GATGGGCACT TCCGGGAGAA GCTGCAAGCT 
GCCAACGCTG AGGACATTAA GAGTGGAAAG CTGAGTCAAG AGCTGGACTT CGTCAGCCAC 
AACGTCCGCA CCAAGCTGGA TGAGCTCAAG CGACAGGAGG TATCAAGGCT TCGGATGCTG 
CTCAAGGCCA AGATGGATGC AAAGCAGGAG CCTAACTTGC AGGTGGACCA CATGAACCTT 
CTGAAGCAGT TTGAACACCT GGACCCTCAG AACCAGCACA CGTTTGAGGC TCGGGACCTA 
GAGCTGCTGA TCCAGACGGC CACCCGAGAC CTCGCCCAGT ATGACGCTGC ACACCATGAA 
GAGTTCAAAC GCTACGAGAT GCTCAAGGAA CATGAGCGAA GACGTTACCT GGAGTCTCTG 
GGAGAGGAGC AGCGGAAGGA GGCTGAGAGG AAGCTACAAG AGCAGCAGCG CAGACACCGG 
GAACACCCCA AAGTCAATGT TCCTGGCAGC CAAGCCCAGT TGAAGGAGGT GTGGGAGGAG 
CTAGATGGAT TGGACCCCAA CAGGTTCAAC CCCAAGACCT TCTTCATACT GCATGACATC 
AACAGCGATG GTGTCCTAGA TGAGCAAGAA CTGGAAGCTC TGTTTACCAA GGAGCTGGAG 
AAGGTGTACG ACCCGAAGAA CGAGGAGGAC GACATGAGGG AGATGGAGGA GGAGCGGCTG 
CGCATGCGGG AGCATGTGAT GAAGAACGTG GACACCAACC AGGACCGACT TGTGACCCTG 
GAGGAATTCC TGGCATCCAC ACAGAGGAAG GAGTTTGGGG AAACTGCGGA GGGATGGAAG 
ACAGTGGAAA TGTATCCAGC CTACACAGAG GAAGAGCTGA AGCGTTTTGA AGAGGAGCTT 
GCTGCCCGGG AGGCTGAGCT AAATGCCAGA GCCCAGCGCC TCAGCCAGGA GACAGAGGCC 
CTGGGGCGCT CCCAGGACCG CCTAGAGGCT CAGAAGAGAG AGCTGCAGCA GGCCGTTCTG 
CAGATGGAGC AAAGGAAGCA GCAACAGCAA GAGCAGAGCG CTCCGCCTTC CCAACCTGAT 
GGGCAGCTGC AGTTCCGTGC AGACACAGGG GATGCTCCTG TCCCAGCTCC AGCAGGTGAC 
CAGAAAGACG TGCCTGCTTC TGAAAAGAAG GTTCCAGAGC AGCCCCCTGT GCTGCCACAG 
CTGGATTCTC AGCATTTATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017445291.1
CDS	14..1414
Translation	MKIEPATMPTSVPRGAPFLLLPPLLMLSAVLAVPVDRAAPHQEDNQATETPDTGLYYHRYLQEVINVLETDGHFREKLQAANAEDIKSGKLSQELDFVSHNVRTKLDELKRQEVSRLRMLLKAKMDAKQEPNLQVDHMNLLKQFEHLDPQNQHTFEARDLELLIQTATRDLAQYDAAHHEEFKRYEMLKEHERRRYLESLGEEQRKEAERKLQEQQRRHREHPKVNVPGSQAQLKEVWEELDGLDPNRFNPKTFFILHDINSDGVLDEQELEALFTKELEKVYDPKNEEDDMREMEEERLRMREHVMKNVDTNQDRLVTLEEFLASTQRKEFGETAEGWKTVEMYPAYTEEELKRFEEELAAREAELNARAQRLSQETEALGRSQDRLEAQKRELQQAVLQMEQRKQQQQEQSAPPSQPDGQLQFRADTGDAPVPAPAGDQKDVPASEKKVPEQPPVLPQLDSQHL

Target ORF information:

RefSeq Version	XM_017589802.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus nucleobindin 1 (Nucb1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017589802.1

ORF Insert Sequence:

ATGAAAATTG AACCAGCTAC GATGCCTACC TCTGTGCCCC GCGGGGCGCC TTTCCTTCTC 
CTACCACCTT TACTGATGCT GTCTGCTGTG CTGGCAGTGC CTGTGGACCG CGCAGCACCT 
CATCAGGAGG ACAACCAGGC CACTGAGACC CCGGACACAG GCCTGTACTA CCATCGGTAC 
CTCCAGGAGG TCATCAACGT GCTAGAGACA GATGGGCACT TCCGGGAGAA GCTGCAAGCT 
GCCAACGCTG AGGACATTAA GAGTGGAAAG CTGAGTCAAG AGCTGGACTT CGTCAGCCAC 
AACGTCCGCA CCAAGCTGGA TGAGCTCAAG CGACAGGAGG TATCAAGGCT TCGGATGCTG 
CTCAAGGCCA AGATGGATGC AAAGCAGGAG CCTAACTTGC AGGTGGACCA CATGAACCTT 
CTGAAGCAGT TTGAACACCT GGACCCTCAG AACCAGCACA CGTTTGAGGC TCGGGACCTA 
GAGCTGCTGA TCCAGACGGC CACCCGAGAC CTCGCCCAGT ATGACGCTGC ACACCATGAA 
GAGTTCAAAC GCTACGAGAT GCTCAAGGAA CATGAGCGAA GACGTTACCT GGAGTCTCTG 
GGAGAGGAGC AGCGGAAGGA GGCTGAGAGG AAGCTACAAG AGCAGCAGCG CAGACACCGG 
GAACACCCCA AAGTCAATGT TCCTGGCAGC CAAGCCCAGT TGAAGGAGGT GTGGGAGGAG 
CTAGATGGAT TGGACCCCAA CAGGTTCAAC CCCAAGACCT TCTTCATACT GCATGACATC 
AACAGCGATG GTGTCCTAGA TGAGCAAGAA CTGGAAGCTC TGTTTACCAA GGAGCTGGAG 
AAGGTGTACG ACCCGAAGAA CGAGGAGGAC GACATGAGGG AGATGGAGGA GGAGCGGCTG 
CGCATGCGGG AGCATGTGAT GAAGAACGTG GACACCAACC AGGACCGACT TGTGACCCTG 
GAGGAATTCC TGGCATCCAC ACAGAGGAAG GAGTTTGGGG AAACTGCGGA GGGATGGAAG 
ACAGTGGAAA TGTATCCAGC CTACACAGAG GAAGAGCTGA AGCGTTTTGA AGAGGAGCTT 
GCTGCCCGGG AGGCTGAGCT AAATGCCAGA GCCCAGCGCC TCAGCCAGGA GACAGAGGCC 
CTGGGGCGCT CCCAGGACCG CCTAGAGGCT CAGAAGAGAG AGCTGCAGCA GGCCGTTCTG 
CAGATGGAGC AAAGGAAGCA GCAACAGCAA GAGCAGAGCG CTCCGCCTTC CCAACCTGAT 
GGGCAGCTGC AGTTCCGTGC AGACACAGGG GATGCTCCTG TCCCAGCTCC AGCAGGTGAC 
CAGAAAGACG TGCCTGCTTC TGAAAAGAAG GTTCCAGAGC AGCCCCCTGT GCTGCCACAG 
CTGGATTCTC AGCATTTATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Nucleobindin 1 (NUCB1) is a Golgi-resident marker of neurons.
Neuroscience314179-88(2016 Feb)
Tulke S,Williams P,Hellysaz A,Ilegems E,Wendel M,Broberger C

The novel neuropeptide phoenixin is highly co-expressed with nesfatin-1 in the rat hypothalamus, an immunohistochemical study.
Neuroscience letters59217-21(2015 Apr)
Pa?asz A,Rojczyk E,Bogus K,Worthington JJ,Wiaderkiewicz R

Modulation of food intake by mTOR signalling in the dorsal motor nucleus of the vagus in male rats: focus on ghrelin and nesfatin-1.
Experimental physiology98(12)1696-704(2013 Dec)
Zhang W,Zhang C,Fritze D,Chai B,Li J,Mulholland MW

G Protein binding sites on Calnuc (nucleobindin 1) and NUCB2 (nucleobindin 2) define a new class of G(alpha)i-regulatory motifs.
The Journal of biological chemistry286(32)28138-49(2011 Aug)
Garcia-Marcos M,Kietrsunthorn PS,Wang H,Ghosh P,Farquhar MG

Nucleobindin 1 is a calcium-regulated guanine nucleotide dissociation inhibitor of G{alpha}i1.
The Journal of biological chemistry285(41)31647-60(2010 Oct)
Kapoor N,Gupta R,Menon ST,Folta-Stogniew E,Raleigh DP,Sakmar TP

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Nucb1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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