Rims1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Rims1
Entrez Gene ID	84556
Full Name	regulating synaptic membrane exocytosis 1
Synonyms	RIM1a,Rim1
General protein information	Preferred Names regulating synaptic membrane exocytosis 1 Names regulating synaptic membrane exocytosis protein 1 RIM 1 Rab3 effector Rim1b protein rab-3-interacting molecule 1 rab3-interacting molecule 1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 9 Map Location: 9q13
Summary	interacts with synaptic GTP-binding protein Rab3s; may regulate exocytosis at the synapse [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_017596655.1	XP_017452144.1	regulating synaptic membrane exocytosis protein 1 isoform X1
XM_017596663.1	XP_017452152.1	regulating synaptic membrane exocytosis protein 1 isoform X10
XM_017596665.1	XP_017452154.1	regulating synaptic membrane exocytosis protein 1 isoform X12
NM_052829.1	NP_439894.1	regulating synaptic membrane exocytosis protein 1
XM_017596659.1	XP_017452148.1	regulating synaptic membrane exocytosis protein 1 isoform X5
XM_017596668.1	XP_017452157.1	regulating synaptic membrane exocytosis protein 1 isoform X15
XM_017596669.1	XP_017452158.1	regulating synaptic membrane exocytosis protein 1 isoform X16
XM_017596671.1	XP_017452160.1	regulating synaptic membrane exocytosis protein 1 isoform X18
XM_017596672.1	XP_017452161.1	regulating synaptic membrane exocytosis protein 1 isoform X19
XM_017596673.1	XP_017452162.1	regulating synaptic membrane exocytosis protein 1 isoform X20
XM_017596676.1	XP_017452165.1	regulating synaptic membrane exocytosis protein 1 isoform X23
XM_017596657.1	XP_017452146.1	regulating synaptic membrane exocytosis protein 1 isoform X3
XM_017596661.1	XP_017452150.1	regulating synaptic membrane exocytosis protein 1 isoform X8
XM_017596666.1	XP_017452155.1	regulating synaptic membrane exocytosis protein 1 isoform X13
XM_017596677.1	XP_017452166.1	regulating synaptic membrane exocytosis protein 1 isoform X24
XM_017596656.1	XP_017452145.1	regulating synaptic membrane exocytosis protein 1 isoform X2
XM_017596658.1	XP_017452147.1	regulating synaptic membrane exocytosis protein 1 isoform X4
XM_017596667.1	XP_017452156.1	regulating synaptic membrane exocytosis protein 1 isoform X14
XM_017596674.1	XP_017452163.1	regulating synaptic membrane exocytosis protein 1 isoform X21
XM_017596660.1	XP_017452149.1	regulating synaptic membrane exocytosis protein 1 isoform X6
XM_008766936.2	XP_008765158.1	regulating synaptic membrane exocytosis protein 1 isoform X7
XM_017596662.1	XP_017452151.1	regulating synaptic membrane exocytosis protein 1 isoform X9
XM_017596664.1	XP_017452153.1	regulating synaptic membrane exocytosis protein 1 isoform X11
XM_017596670.1	XP_017452159.1	regulating synaptic membrane exocytosis protein 1 isoform X17
XM_017596675.1	XP_017452164.1	regulating synaptic membrane exocytosis protein 1 isoform X22
XM_017596678.1	XP_017452167.1	regulating synaptic membrane exocytosis protein 1 isoform X25
XM_017596679.1	XP_017452168.1	regulating synaptic membrane exocytosis protein 1 isoform X26

Pathways from BioSystems

Homologies

Bos taurus (cattle)	RIMS1	XP_005210703.1
Mus musculus (house mouse)	Rims1	NP_444500.1
Rattus norvegicus (Norway rat)	Rims1	NP_439894.1
Gallus gallus (chicken)	RIMS1	XP_004940491.1
Danio rerio (zebrafish)	si:dkey-179o14.1	XP_005168452.1
	RIMS1	NP_001032357.1
Canis lupus familiaris (dog)	RIMS1	XP_005627828.1
Xenopus tropicalis (tropical clawed frog)	rims1	NP_001096180.1
Homo sapiens (human)	RIMS1	NP_055804.2
Pan troglodytes (chimpanzee)	RIMS1	XP_003311440.2

Gene Ontology
Bibliography

Related articles in PubMed

Fusion Competent Synaptic Vesicles Persist upon Active Zone Disruption and Loss of Vesicle Docking.
Wang SSH, Held RG, Wong MY, Liu C, Karakhanyan A, Kaeser PS
Neuron91(4)777-791(2016 Aug)

A trans-synaptic nanocolumn aligns neurotransmitter release to receptors.
Tang AH, Chen H, Li TP, Metzbower SR, MacGillavry HD, Blanpied TA
Nature536(7615)210-4(2016 08)

Protein mutated in paroxysmal dyskinesia interacts with the active zone protein RIM and suppresses synaptic vesicle exocytosis.
Shen Y, Ge WP, Li Y, Hirano A, Lee HY, Rohlmann A, Missler M, Tsien RW, Jan LY, Fu YH, Pt??ek LJ
Proceedings of the National Academy of Sciences of the United States of America112(10)2935-41(2015 Mar)

RIM1? SUMOylation is required for fast synaptic vesicle exocytosis.
Girach F, Craig TJ, Rocca DL, Henley JM
Cell reports5(5)1294-301(2013 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

RIM1alpha SUMOylation at K502 is required for its binding to Cav2.1 and for fast synaptic vesicle exocytosis.
Title: RIM1? SUMOylation is required for fast synaptic vesicle exocytosis.

Depletion of liprin-alpha2 resulted in reduced turnover of RIM1 and CASK at presynaptic terminals, suggesting that liprin-alpha2 promotes dynamic scaffolding for molecular complexes that facilitate synaptic vesicle release.
Title: Liprin-?2 promotes the presynaptic recruitment and turnover of RIM1/CASK to facilitate synaptic transmission.

Data show that Bassoon colocalizes with Aczonin at conventional synapses, but in ribbon synapses, CAST, Munc13, and RIM are segregated from Aczonin, and Aczonin is positioned and may control the access of neurotransmitter vesicles to the fusion site.
Title: Molecular in situ topology of Aczonin/Piccolo and associated proteins at the mammalian neurotransmitter release site.

Presynaptic silencing was accompanied by decreases in levels of the priming proteins Munc13-1 and Rim1.
Title: A role for the ubiquitin-proteasome system in activity-dependent presynaptic silencing.

The crystal structure of the RIM1alpha C2B domain dimer reveals that it forms a beta-sandwich and that the unique N- and C-terminal extensions form a small subdomain that packs against the beta-sandwich and mediates dimerization.
Title: Crystal structure of the RIM1alpha C2B domain at 1.7 A resolution.

The following Rims1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Rims1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa62894	XM_017596677.1 Latest version!	Rattus norvegicus regulating synaptic membrane exocytosis 1 (Rims1), transcript variant X24, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
ORa62891	XM_017596674.1 Latest version!	Rattus norvegicus regulating synaptic membrane exocytosis 1 (Rims1), transcript variant X21, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ORa62892	XM_017596675.1 Latest version!	Rattus norvegicus regulating synaptic membrane exocytosis 1 (Rims1), transcript variant X22, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ORa62893	XM_017596676.1 Latest version!	Rattus norvegicus regulating synaptic membrane exocytosis 1 (Rims1), transcript variant X23, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ORa09170	NM_052829.1 Latest version!	Rattus norvegicus regulating synaptic membrane exocytosis 1 (Rims1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00
ORa43850	XM_008766936.2 Latest version!	Rattus norvegicus regulating synaptic membrane exocytosis 1 (Rims1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00
ORa62872	XM_017596655.1 Latest version!	Rattus norvegicus regulating synaptic membrane exocytosis 1 (Rims1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00
ORa62873	XM_017596656.1 Latest version!	Rattus norvegicus regulating synaptic membrane exocytosis 1 (Rims1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00
ORa62874	XM_017596657.1 Latest version!	Rattus norvegicus regulating synaptic membrane exocytosis 1 (Rims1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00
ORa62875	XM_017596658.1 Latest version!	Rattus norvegicus regulating synaptic membrane exocytosis 1 (Rims1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00
ORa62876	XM_017596659.1 Latest version!	Rattus norvegicus regulating synaptic membrane exocytosis 1 (Rims1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00
ORa62877	XM_017596660.1 Latest version!	Rattus norvegicus regulating synaptic membrane exocytosis 1 (Rims1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00
ORa62878	XM_017596661.1 Latest version!	Rattus norvegicus regulating synaptic membrane exocytosis 1 (Rims1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00
ORa62879	XM_017596662.1 Latest version!	Rattus norvegicus regulating synaptic membrane exocytosis 1 (Rims1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00
ORa62880	XM_017596663.1 Latest version!	Rattus norvegicus regulating synaptic membrane exocytosis 1 (Rims1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00
ORa62881	XM_017596664.1 Latest version!	Rattus norvegicus regulating synaptic membrane exocytosis 1 (Rims1), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00
ORa62882	XM_017596665.1 Latest version!	Rattus norvegicus regulating synaptic membrane exocytosis 1 (Rims1), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00
ORa62883	XM_017596666.1 Latest version!	Rattus norvegicus regulating synaptic membrane exocytosis 1 (Rims1), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00
ORa62884	XM_017596667.1 Latest version!	Rattus norvegicus regulating synaptic membrane exocytosis 1 (Rims1), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00
ORa62885	XM_017596668.1 Latest version!	Rattus norvegicus regulating synaptic membrane exocytosis 1 (Rims1), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00
ORa62886	XM_017596669.1 Latest version!	Rattus norvegicus regulating synaptic membrane exocytosis 1 (Rims1), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$727.30 $1039.00
ORa62887	XM_017596670.1 Latest version!	Rattus norvegicus regulating synaptic membrane exocytosis 1 (Rims1), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$727.30 $1039.00
ORa62888	XM_017596671.1 Latest version!	Rattus norvegicus regulating synaptic membrane exocytosis 1 (Rims1), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$727.30 $1039.00
ORa62889	XM_017596672.1 Latest version!	Rattus norvegicus regulating synaptic membrane exocytosis 1 (Rims1), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$727.30 $1039.00
ORa62890	XM_017596673.1 Latest version!	Rattus norvegicus regulating synaptic membrane exocytosis 1 (Rims1), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$727.30 $1039.00
ORa62895	XM_017596678.1 Latest version!	Rattus norvegicus regulating synaptic membrane exocytosis 1 (Rims1), transcript variant X25, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00
ORa62896	XM_017596679.1 Latest version!	Rattus norvegicus regulating synaptic membrane exocytosis 1 (Rims1), transcript variant X26, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$727.30 $1039.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Rims1 ( XM_017596677.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017452166.1 Rattus norvegicus regulating synaptic membrane exocytosis 1 (Rims1), transcript variant X24, mRNA.

Price & Availability↑

CloneID	ORa62894
Clone ID Related Accession (Same CDS sequence)	XM_017596677.1
Accession Version	XM_017596677.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 660bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	regulating synaptic membrane exocytosis protein 1 isoform X24
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005108.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGCTG AAATGCGGAA GATGGTGAGA CAGCCGAGCC GGGAGTCCAC GGATGGCAGC 
ATCAACAGTT ATAGCTCGGA AGGAAACTTG ATATTTCCTG GAGTTCGAGT AGGACCCGAC 
AGTCAGTTCA GTGATTTCCT TGATGGGTTG GGACCAGCGC AGCTCGTTGG CCGTCAGACG 
CTCGCCACCC CGGCCATGGG CGATATCCAA ATCGGGATGG AGGATAAGAA GGGTCAGTTG 
GAGGTTGAGG TTATCAGAGC CCGGAGCCTT ACACAAAAAC CTGGTTCCAA ATCTACACCC 
GCTCCCTATG TGAAAGTATA TCTTTTGGAA AATGGAGCCT GTATTGCCAA AAAGAAGACA 
AGAATTGCAC GGAAAACTCT CGATCCTTTG TATCAGCAGT CCCTGGTTTT TGATGAAAGT 
CCACAGGGTA AAGTTCTTCA GGTGATTGTC TGGGGTGACT ATGGAAGAAT GGACCACAAA 
TGCTTTATGG GTGTGGCTCA AATCTTGTTG GAAGAACTTG ATCTATCCAG CATGGTGATT 
GGATGGTATA AATTGTTCCC TCCGTCCTCA CTGGTGGATC CCACTCTCGC TCCCCTGACC 
CGCCGGGCTT CCCAATCATC TCTGGAAAGT TCGTCCGGGC CTCCCTGCAT CCGGTCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017452166.1
CDS	219..878
Translation	MAAEMRKMVRQPSRESTDGSINSYSSEGNLIFPGVRVGPDSQFSDFLDGLGPAQLVGRQTLATPAMGDIQIGMEDKKGQLEVEVIRARSLTQKPGSKSTPAPYVKVYLLENGACIAKKKTRIARKTLDPLYQQSLVFDESPQGKVLQVIVWGDYGRMDHKCFMGVAQILLEELDLSSMVIGWYKLFPPSSLVDPTLAPLTRRASQSSLESSSGPPCIRS

Target ORF information:

RefSeq Version	XM_017596677.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus regulating synaptic membrane exocytosis 1 (Rims1), transcript variant X24, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017596677.1

ORF Insert Sequence:

ATGGCAGCTG AAATGCGGAA GATGGTGAGA CAGCCGAGCC GGGAGTCCAC GGATGGCAGC 
ATCAACAGTT ATAGCTCGGA AGGAAACTTG ATATTTCCTG GAGTTCGAGT AGGACCCGAC 
AGTCAGTTCA GTGATTTCCT TGATGGGTTG GGACCAGCGC AGCTCGTTGG CCGTCAGACG 
CTCGCCACCC CGGCCATGGG CGATATCCAA ATCGGGATGG AGGATAAGAA GGGTCAGTTG 
GAGGTTGAGG TTATCAGAGC CCGGAGCCTT ACACAAAAAC CTGGTTCCAA ATCTACACCC 
GCTCCCTATG TGAAAGTATA TCTTTTGGAA AATGGAGCCT GTATTGCCAA AAAGAAGACA 
AGAATTGCAC GGAAAACTCT CGATCCTTTG TATCAGCAGT CCCTGGTTTT TGATGAAAGT 
CCACAGGGTA AAGTTCTTCA GGTGATTGTC TGGGGTGACT ATGGAAGAAT GGACCACAAA 
TGCTTTATGG GTGTGGCTCA AATCTTGTTG GAAGAACTTG ATCTATCCAG CATGGTGATT 
GGATGGTATA AATTGTTCCC TCCGTCCTCA CTGGTGGATC CCACTCTCGC TCCCCTGACC 
CGCCGGGCTT CCCAATCATC TCTGGAAAGT TCGTCCGGGC CTCCCTGCAT CCGGTCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rims1 ( XM_017596674.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017452163.1 Rattus norvegicus regulating synaptic membrane exocytosis 1 (Rims1), transcript variant X21, mRNA.

Price & Availability↑

CloneID	ORa62891
Clone ID Related Accession (Same CDS sequence)	XM_017596674.1
Accession Version	XM_017596674.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3345bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	regulating synaptic membrane exocytosis protein 1 isoform X21
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005108.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTGCTG TGATGTGTGC ATCTGGGAGG CCCGTCCCTG CAGAAGGGTG GGGAGAAGAA 
CTGAGGTCTG TTGATTCTGA GGCGGGACCA AGTGAGACCC ACCGAACATG TGCTGGTGAC 
TTGGATTACT ACTGGTTGGA TCCCGCCACG TGGCACAGCA GGGAAACGTC GCCTATCAGT 
TCGCATCCTG TAACGTGGCA GCCGTCTAAA GAGGGAGATC GACTAATCGG CCGTGTTATT 
CTTAACAAAA GAACAACCAT GCCCAAAGAA TCAGGTGCAT TATTGGGTCT GAAGGTGGTT 
GGAGGAAAAA TGACGGACTT AGGGCGCCTT GGTGCTTTCA TCACCAAAGT AAAGAAGGGC 
AGCCTGGCAG ACGTCGTCGG ACACCTAAGA GCAGGGGACG AAGTCCTAGA GTGGAATGGT 
AAACCCCTGC CGGGAGCAAC AAACGAAGAA GTTTACAACA TTATCTTAGA ATCAAAATCA 
GAACCTCAAG TTGAGATTAT TGTTTCAAGG CCTATTGGTG ACATCCCCAG GATCCCTGAG 
AGTTCCCATC CTCCCCTGGA GTCCAGTTCA AGTTCCTTTG AATCTCAGAA AATGGAAAGG 
CCTTCTATTT CTGTTATTTC TCCAACCAGC CCTGGAGCTC TGAAAGATGC CCCACAAGTC 
TTACCAGGGC AACTCTCAGT GAAGCTATGG TATGATAAAG TGGGGCACCA GCTGATTGTA 
AATGTTCTAC AAGCAACAGA TCTACCCCCT AGAGTAGATG GCCGTCCCAG GAATCCCTAT 
GTAAAAATGT ATTTTCTTCC AGATAGAAGC GACAAAAGTA AAAGGAGAAC CAAAACAGTA 
AAGAAACTTC TAGAGCCAAA ATGGAACCAG ACATTTGTCT ACTCACACGT ACATCGTAGA 
GATTTTCGAG AGCGAATGTT AGAGATTACC GTGTGGGACC AGCCGAGAGT ACAGGACGAA 
GAGAGTGAAT TTCTTGGAGA GATCCTCATA GAGTTGGAAA CAGCGCTTTT AGATGATGAG 
CCCCATTGGT ATAAACTCCA GACACATGAC GAATCTTCAC TACCTCTGCC TCAGCCATCA 
CCGTTCATGC CCAGGCGGCA TATTCATGGA GAGAGCTCCA GCAAAAAGCT ACAAAGATCT 
CAGCGAATCA GTGATAGTGA CATCTCAGAT TATGAGGTTG ATGATGGTAT TGGAGTAGTG 
CCTCCAGTGG GTTATAGAGC TAGTGCTAGA GAGAGTAAAG CAACCACGTT AACAGTGCCA 
GAGCAACAAA GAACTACACA TCACCGCTCA CGTTCCGTGT CTCCTCATCG CGGCGATGAT 
CAGGGAAGGC CTCGTTCACG TTTACCAAAT GTGCCATTAC AGAGGAGCTT AGATGAAATT 
CATCCAACAC GAAGGTCACG TTCTCCAACC CGACACCATG ATGCCTCCCG AAGCCCGGCC 
GATCACAGAT CCAGACATGT GGAAAGTCAA TATTCGTCAG AGCCAGACAG TGAGCTTCTC 
ATGCTGCCCA GAGCAAAACG AGGACGAAGT GCAGAAAGCC TACACATGAC CAGTGAACTG 
CAGCCCTCTC TTGACAGGGC TAGGAGTGCT AGTACCAACT GCTTGAGACC AGATACTAGT 
TTGCATTCAC CAGAACGAGA AAGGGGTAGA TGGTCCCCCT CCCTAGATAG GAGGCGACCT 
GCTAGCCCCA GGATTCAAAT CCAGCATGCA TCTCCGGAGA ATGACAGGCA CTCCAGAAAG 
TCTGAAAGAT GTAGCATCCA AAAACAGTCT AGGAAAGGCA CAGCCTCTGA TGCAGACAGG 
GTTCTCCCAC CATGCCTTTC TAGAAGGGGA TACGCAACCC CAAGAGCAAC CGATCAACCG 
GTCGTTAGGG GAAAGCATCC CACTCGTTCA CGGTCGAGCG AGCACTCTAG TGTCAGAACC 
CTGTGTTCTA TGCACCACCT TGCCCCCGGA GGGTCGGCGC CACCTTCTCC ACTTCTGACA 
AGAACGCACC GACAAGGAAG CCCAACCCAG TCTCCTCCAG CAGACACATC CTTCGGCAGT 
CGCCGTGGAA GACAGCTCCC ACAGGTGCCA GTTCGAAGCG GCAGTATAGA ACAAGCAAGC 
TTAGTAGTGG AGGAGCGAAC GAGACAGATG AAAATGAAAG TTCACCGATT TAAGCAGACA 
ACAGGGTCTG GGTCTAGTCA AGAACTTGAC CACGAGCAAT ACTCCAAGAA GTCACGTTAC 
ATTATGGAGT ACAACATACA TAAAGATCAG TACAGAAGCT GTGATAACGC GTCTGCCAAG 
TCTTCAGATA GTGATGTCAG TGATGTGTCC GCCATTTCCA GAGCCAGCAG TACCTCACGC 
CTCAGCAGCA CAAGCTTTAT GTCAGAGCAG TCTGAGCGCC CCAGGGGTAG GATCAGTTCA 
TTTACCCCCA AAATGCAAGG CAGACGGATG GGGACTTCAG GAAGAGCCAT CATCAAGAGC 
ACCAGTGTAA GTGGAGAGAT ATATACACTG GAACGTAATG ACGGTAGCCA GTCGGACACG 
GCCGTGGGTA CCGTCGGAGC CGGTGGAAAG AAACGAAGAT CCAGCCTGAG CGCCAAAGTG 
GTAGCCATTG TGTCTCGAAG AAGCAGGAGC ACGTCACAGC TCAGCCAGAC AGAGTCGGGC 
CACAAGAAGT TGAAAAGCAC CATCCAGAGG AGTACGGAAA CAGGAATGGC AGCTGAAATG 
CGGAAGATGG TGAGACAGCC GAGCCGGGAG TCCACGGATG GCAGCATCAA CAGTTATAGC 
TCGGAAGGAA ACTTGATATT TCCTGGAGTT CGAGTAGGAC CCGACAGTCA GTTCAGTGAT 
TTCCTTGATG GGTTGGGACC AGCGCAGCTC GTTGGCCGTC AGACGCTCGC CACCCCGGCC 
ATGGGCGATA TCCAAATCGG GATGGAGGAT AAGAAGGGTC AGTTGGAGGT TGAGGTTATC 
AGAGCCCGGA GCCTTACACA AAAACCTGGT TCCAAATCTA CACCCGCTCC CTATGTGAAA 
GTATATCTTT TGGAAAATGG AGCCTGTATT GCCAAAAAGA AGACAAGAAT TGCACGGAAA 
ACTCTCGATC CTTTGTATCA GCAGTCCCTG GTTTTTGATG AAAGTCCACA GGGTAAAGTT 
CTTCAGGTGA TTGTCTGGGG TGACTATGGA AGAATGGACC ACAAATGCTT TATGGGTGTG 
GCTCAAATCT TGTTGGAAGA ACTTGATCTA TCCAGCATGG TGATTGGATG GTATAAATTG 
TTCCCTCCGT CCTCACTGGT GGATCCCACT CTCGCTCCCC TGACCCGCCG GGCTTCCCAA 
TCATCTCTGG AAAGTTCGTC CGGGCCTCCC TGCATCCGGT CATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017452163.1
CDS	675..4019
Translation	MVAVMCASGRPVPAEGWGEELRSVDSEAGPSETHRTCAGDLDYYWLDPATWHSRETSPISSHPVTWQPSKEGDRLIGRVILNKRTTMPKESGALLGLKVVGGKMTDLGRLGAFITKVKKGSLADVVGHLRAGDEVLEWNGKPLPGATNEEVYNIILESKSEPQVEIIVSRPIGDIPRIPESSHPPLESSSSSFESQKMERPSISVISPTSPGALKDAPQVLPGQLSVKLWYDKVGHQLIVNVLQATDLPPRVDGRPRNPYVKMYFLPDRSDKSKRRTKTVKKLLEPKWNQTFVYSHVHRRDFRERMLEITVWDQPRVQDEESEFLGEILIELETALLDDEPHWYKLQTHDESSLPLPQPSPFMPRRHIHGESSSKKLQRSQRISDSDISDYEVDDGIGVVPPVGYRASARESKATTLTVPEQQRTTHHRSRSVSPHRGDDQGRPRSRLPNVPLQRSLDEIHPTRRSRSPTRHHDASRSPADHRSRHVESQYSSEPDSELLMLPRAKRGRSAESLHMTSELQPSLDRARSASTNCLRPDTSLHSPERERGRWSPSLDRRRPASPRIQIQHASPENDRHSRKSERCSIQKQSRKGTASDADRVLPPCLSRRGYATPRATDQPVVRGKHPTRSRSSEHSSVRTLCSMHHLAPGGSAPPSPLLTRTHRQGSPTQSPPADTSFGSRRGRQLPQVPVRSGSIEQASLVVEERTRQMKMKVHRFKQTTGSGSSQELDHEQYSKKSRYIMEYNIHKDQYRSCDNASAKSSDSDVSDVSAISRASSTSRLSSTSFMSEQSERPRGRISSFTPKMQGRRMGTSGRAIIKSTSVSGEIYTLERNDGSQSDTAVGTVGAGGKKRRSSLSAKVVAIVSRRSRSTSQLSQTESGHKKLKSTIQRSTETGMAAEMRKMVRQPSRESTDGSINSYSSEGNLIFPGVRVGPDSQFSDFLDGLGPAQLVGRQTLATPAMGDIQIGMEDKKGQLEVEVIRARSLTQKPGSKSTPAPYVKVYLLENGACIAKKKTRIARKTLDPLYQQSLVFDESPQGKVLQVIVWGDYGRMDHKCFMGVAQILLEELDLSSMVIGWYKLFPPSSLVDPTLAPLTRRASQSSLESSSGPPCIRS

Target ORF information:

RefSeq Version	XM_017596674.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus regulating synaptic membrane exocytosis 1 (Rims1), transcript variant X21, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017596674.1

ORF Insert Sequence:

ATGGTTGCTG TGATGTGTGC ATCTGGGAGG CCCGTCCCTG CAGAAGGGTG GGGAGAAGAA 
CTGAGGTCTG TTGATTCTGA GGCGGGACCA AGTGAGACCC ACCGAACATG TGCTGGTGAC 
TTGGATTACT ACTGGTTGGA TCCCGCCACG TGGCACAGCA GGGAAACGTC GCCTATCAGT 
TCGCATCCTG TAACGTGGCA GCCGTCTAAA GAGGGAGATC GACTAATCGG CCGTGTTATT 
CTTAACAAAA GAACAACCAT GCCCAAAGAA TCAGGTGCAT TATTGGGTCT GAAGGTGGTT 
GGAGGAAAAA TGACGGACTT AGGGCGCCTT GGTGCTTTCA TCACCAAAGT AAAGAAGGGC 
AGCCTGGCAG ACGTCGTCGG ACACCTAAGA GCAGGGGACG AAGTCCTAGA GTGGAATGGT 
AAACCCCTGC CGGGAGCAAC AAACGAAGAA GTTTACAACA TTATCTTAGA ATCAAAATCA 
GAACCTCAAG TTGAGATTAT TGTTTCAAGG CCTATTGGTG ACATCCCCAG GATCCCTGAG 
AGTTCCCATC CTCCCCTGGA GTCCAGTTCA AGTTCCTTTG AATCTCAGAA AATGGAAAGG 
CCTTCTATTT CTGTTATTTC TCCAACCAGC CCTGGAGCTC TGAAAGATGC CCCACAAGTC 
TTACCAGGGC AACTCTCAGT GAAGCTATGG TATGATAAAG TGGGGCACCA GCTGATTGTA 
AATGTTCTAC AAGCAACAGA TCTACCCCCT AGAGTAGATG GCCGTCCCAG GAATCCCTAT 
GTAAAAATGT ATTTTCTTCC AGATAGAAGC GACAAAAGTA AAAGGAGAAC CAAAACAGTA 
AAGAAACTTC TAGAGCCAAA ATGGAACCAG ACATTTGTCT ACTCACACGT ACATCGTAGA 
GATTTTCGAG AGCGAATGTT AGAGATTACC GTGTGGGACC AGCCGAGAGT ACAGGACGAA 
GAGAGTGAAT TTCTTGGAGA GATCCTCATA GAGTTGGAAA CAGCGCTTTT AGATGATGAG 
CCCCATTGGT ATAAACTCCA GACACATGAC GAATCTTCAC TACCTCTGCC TCAGCCATCA 
CCGTTCATGC CCAGGCGGCA TATTCATGGA GAGAGCTCCA GCAAAAAGCT ACAAAGATCT 
CAGCGAATCA GTGATAGTGA CATCTCAGAT TATGAGGTTG ATGATGGTAT TGGAGTAGTG 
CCTCCAGTGG GTTATAGAGC TAGTGCTAGA GAGAGTAAAG CAACCACGTT AACAGTGCCA 
GAGCAACAAA GAACTACACA TCACCGCTCA CGTTCCGTGT CTCCTCATCG CGGCGATGAT 
CAGGGAAGGC CTCGTTCACG TTTACCAAAT GTGCCATTAC AGAGGAGCTT AGATGAAATT 
CATCCAACAC GAAGGTCACG TTCTCCAACC CGACACCATG ATGCCTCCCG AAGCCCGGCC 
GATCACAGAT CCAGACATGT GGAAAGTCAA TATTCGTCAG AGCCAGACAG TGAGCTTCTC 
ATGCTGCCCA GAGCAAAACG AGGACGAAGT GCAGAAAGCC TACACATGAC CAGTGAACTG 
CAGCCCTCTC TTGACAGGGC TAGGAGTGCT AGTACCAACT GCTTGAGACC AGATACTAGT 
TTGCATTCAC CAGAACGAGA AAGGGGTAGA TGGTCCCCCT CCCTAGATAG GAGGCGACCT 
GCTAGCCCCA GGATTCAAAT CCAGCATGCA TCTCCGGAGA ATGACAGGCA CTCCAGAAAG 
TCTGAAAGAT GTAGCATCCA AAAACAGTCT AGGAAAGGCA CAGCCTCTGA TGCAGACAGG 
GTTCTCCCAC CATGCCTTTC TAGAAGGGGA TACGCAACCC CAAGAGCAAC CGATCAACCG 
GTCGTTAGGG GAAAGCATCC CACTCGTTCA CGGTCGAGCG AGCACTCTAG TGTCAGAACC 
CTGTGTTCTA TGCACCACCT TGCCCCCGGA GGGTCGGCGC CACCTTCTCC ACTTCTGACA 
AGAACGCACC GACAAGGAAG CCCAACCCAG TCTCCTCCAG CAGACACATC CTTCGGCAGT 
CGCCGTGGAA GACAGCTCCC ACAGGTGCCA GTTCGAAGCG GCAGTATAGA ACAAGCAAGC 
TTAGTAGTGG AGGAGCGAAC GAGACAGATG AAAATGAAAG TTCACCGATT TAAGCAGACA 
ACAGGGTCTG GGTCTAGTCA AGAACTTGAC CACGAGCAAT ACTCCAAGAA GTCACGTTAC 
ATTATGGAGT ACAACATACA TAAAGATCAG TACAGAAGCT GTGATAACGC GTCTGCCAAG 
TCTTCAGATA GTGATGTCAG TGATGTGTCC GCCATTTCCA GAGCCAGCAG TACCTCACGC 
CTCAGCAGCA CAAGCTTTAT GTCAGAGCAG TCTGAGCGCC CCAGGGGTAG GATCAGTTCA 
TTTACCCCCA AAATGCAAGG CAGACGGATG GGGACTTCAG GAAGAGCCAT CATCAAGAGC 
ACCAGTGTAA GTGGAGAGAT ATATACACTG GAACGTAATG ACGGTAGCCA GTCGGACACG 
GCCGTGGGTA CCGTCGGAGC CGGTGGAAAG AAACGAAGAT CCAGCCTGAG CGCCAAAGTG 
GTAGCCATTG TGTCTCGAAG AAGCAGGAGC ACGTCACAGC TCAGCCAGAC AGAGTCGGGC 
CACAAGAAGT TGAAAAGCAC CATCCAGAGG AGTACGGAAA CAGGAATGGC AGCTGAAATG 
CGGAAGATGG TGAGACAGCC GAGCCGGGAG TCCACGGATG GCAGCATCAA CAGTTATAGC 
TCGGAAGGAA ACTTGATATT TCCTGGAGTT CGAGTAGGAC CCGACAGTCA GTTCAGTGAT 
TTCCTTGATG GGTTGGGACC AGCGCAGCTC GTTGGCCGTC AGACGCTCGC CACCCCGGCC 
ATGGGCGATA TCCAAATCGG GATGGAGGAT AAGAAGGGTC AGTTGGAGGT TGAGGTTATC 
AGAGCCCGGA GCCTTACACA AAAACCTGGT TCCAAATCTA CACCCGCTCC CTATGTGAAA 
GTATATCTTT TGGAAAATGG AGCCTGTATT GCCAAAAAGA AGACAAGAAT TGCACGGAAA 
ACTCTCGATC CTTTGTATCA GCAGTCCCTG GTTTTTGATG AAAGTCCACA GGGTAAAGTT 
CTTCAGGTGA TTGTCTGGGG TGACTATGGA AGAATGGACC ACAAATGCTT TATGGGTGTG 
GCTCAAATCT TGTTGGAAGA ACTTGATCTA TCCAGCATGG TGATTGGATG GTATAAATTG 
TTCCCTCCGT CCTCACTGGT GGATCCCACT CTCGCTCCCC TGACCCGCCG GGCTTCCCAA 
TCATCTCTGG AAAGTTCGTC CGGGCCTCCC TGCATCCGGT CATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rims1 ( XM_017596675.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017452164.1 Rattus norvegicus regulating synaptic membrane exocytosis 1 (Rims1), transcript variant X22, mRNA.

Price & Availability↑

CloneID	ORa62892
Clone ID Related Accession (Same CDS sequence)	XM_017596675.1
Accession Version	XM_017596675.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3240bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	regulating synaptic membrane exocytosis protein 1 isoform X22
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005108.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAAAGG GTGACTTGGA TTACTACTGG TTGGATCCCG CCACGTGGCA CAGCAGGGAA 
ACGTCGCCTA TCAGTTCGCA TCCTGTAACG TGGCAGCCGT CTAAAGAGGG AGATCGACTA 
ATCGGCCGTG TTATTCTTAA CAAAAGAACA ACCATGCCCA AAGAATCAGG TGCATTATTG 
GGTCTGAAGG TGGTTGGAGG AAAAATGACG GACTTAGGGC GCCTTGGTGC TTTCATCACC 
AAAGTAAAGA AGGGCAGCCT GGCAGACGTC GTCGGACACC TAAGAGCAGG GGACGAAGTC 
CTAGAGTGGA ATGGTAAACC CCTGCCGGGA GCAACAAACG AAGAAGTTTA CAACATTATC 
TTAGAATCAA AATCAGAACC TCAAGTTGAG ATTATTGTTT CAAGGCCTAT TGGTGACATC 
CCCAGGATCC CTGAGAGTTC CCATCCTCCC CTGGAGTCCA GTTCAAGTTC CTTTGAATCT 
CAGAAAATGG AAAGGCCTTC TATTTCTGTT ATTTCTCCAA CCAGCCCTGG AGCTCTGAAA 
GATGCCCCAC AAGTCTTACC AGGGCAACTC TCAGTGAAGC TATGGTATGA TAAAGTGGGG 
CACCAGCTGA TTGTAAATGT TCTACAAGCA ACAGATCTAC CCCCTAGAGT AGATGGCCGT 
CCCAGGAATC CCTATGTAAA AATGTATTTT CTTCCAGATA GAAGCGACAA AAGTAAAAGG 
AGAACCAAAA CAGTAAAGAA ACTTCTAGAG CCAAAATGGA ACCAGACATT TGTCTACTCA 
CACGTACATC GTAGAGATTT TCGAGAGCGA ATGTTAGAGA TTACCGTGTG GGACCAGCCG 
AGAGTACAGG ACGAAGAGAG TGAATTTCTT GGAGAGATCC TCATAGAGTT GGAAACAGCG 
CTTTTAGATG ATGAGCCCCA TTGGTATAAA CTCCAGACAC ATGACGAATC TTCACTACCT 
CTGCCTCAGC CATCACCGTT CATGCCCAGG CGGCATATTC ATGGAGAGAG CTCCAGCAAA 
AAGCTACAAA GATCTCAGCG AATCAGTGAT AGTGACATCT CAGATTATGA GGTTGATGAT 
GGTATTGGAG TAGTGCCTCC AGTGGGTTAT AGAGCTAGTG CTAGAGAGAG TAAAGCAACC 
ACGTTAACAG TGCCAGAGCA ACAAAGAACT ACACATCACC GCTCACGTTC CGTGTCTCCT 
CATCGCGGCG ATGATCAGGG AAGGCCTCGT TCACGTTTAC CAAATGTGCC ATTACAGAGG 
AGCTTAGATG AAATTCATCC AACACGAAGG TCACGTTCTC CAACCCGACA CCATGATGCC 
TCCCGAAGCC CGGCCGATCA CAGATCCAGA CATGTGGAAA GTCAATATTC GTCAGAGCCA 
GACAGTGAGC TTCTCATGCT GCCCAGAGCA AAACGAGGAC GAAGTGCAGA AAGCCTACAC 
ATGACCAGTG AACTGCAGCC CTCTCTTGAC AGGGCTAGGA GTGCTAGTAC CAACTGCTTG 
AGACCAGATA CTAGTTTGCA TTCACCAGAA CGAGAAAGGG GTAGATGGTC CCCCTCCCTA 
GATAGGAGGC GACCTGCTAG CCCCAGGATT CAAATCCAGC ATGCATCTCC GGAGAATGAC 
AGGCACTCCA GAAAGTCTGA AAGATGTAGC ATCCAAAAAC AGTCTAGGAA AGGCACAGCC 
TCTGATGCAG ACAGGGTTCT CCCACCATGC CTTTCTAGAA GGGGATACGC AACCCCAAGA 
GCAACCGATC AACCGGTCGT TAGGGGAAAG CATCCCACTC GTTCACGGTC GAGCGAGCAC 
TCTAGTGTCA GAACCCTGTG TTCTATGCAC CACCTTGCCC CCGGAGGGTC GGCGCCACCT 
TCTCCACTTC TGACAAGAAC GCACCGACAA GGAAGCCCAA CCCAGTCTCC TCCAGCAGAC 
ACATCCTTCG GCAGTCGCCG TGGAAGACAG CTCCCACAGG TGCCAGTTCG AAGCGGCAGT 
ATAGAACAAG CAAGCTTAGT AGTGGAGGAG CGAACGAGAC AGATGAAAAT GAAAGTTCAC 
CGATTTAAGC AGACAACAGG GTCTGGGTCT AGTCAAGAAC TTGACCACGA GCAATACTCC 
AAGAAGTCAC GTTACATTAT GGAGTACAAC ATACATAAAG ATCAGTACAG AAGCTGTGAT 
AACGCGTCTG CCAAGTCTTC AGATAGTGAT GTCAGTGATG TGTCCGCCAT TTCCAGAGCC 
AGCAGTACCT CACGCCTCAG CAGCACAAGC TTTATGTCAG AGCAGTCTGA GCGCCCCAGG 
GGTAGGATCA GTTCATTTAC CCCCAAAATG CAAGGCAGAC GGATGGGGAC TTCAGGAAGA 
GCCATCATCA AGAGCACCAG TGTAAGTGGA GAGATATATA CACTGGAACG TAATGACGGT 
AGCCAGTCGG ACACGGCCGT GGGTACCGTC GGAGCCGGTG GAAAGAAACG AAGATCCAGC 
CTGAGCGCCA AAGTGGTAGC CATTGTGTCT CGAAGAAGCA GGAGCACGTC ACAGCTCAGC 
CAGACAGAGT CGGGCCACAA GAAGTTGAAA AGCACCATCC AGAGGAGTAC GGAAACAGGA 
ATGGCAGCTG AAATGCGGAA GATGGTGAGA CAGCCGAGCC GGGAGTCCAC GGATGGCAGC 
ATCAACAGTT ATAGCTCGGA AGGAAACTTG ATATTTCCTG GAGTTCGAGT AGGACCCGAC 
AGTCAGTTCA GTGATTTCCT TGATGGGTTG GGACCAGCGC AGCTCGTTGG CCGTCAGACG 
CTCGCCACCC CGGCCATGGG CGATATCCAA ATCGGGATGG AGGATAAGAA GGGTCAGTTG 
GAGGTTGAGG TTATCAGAGC CCGGAGCCTT ACACAAAAAC CTGGTTCCAA ATCTACACCC 
GCTCCCTATG TGAAAGTATA TCTTTTGGAA AATGGAGCCT GTATTGCCAA AAAGAAGACA 
AGAATTGCAC GGAAAACTCT CGATCCTTTG TATCAGCAGT CCCTGGTTTT TGATGAAAGT 
CCACAGGGTA AAGTTCTTCA GGTGATTGTC TGGGGTGACT ATGGAAGAAT GGACCACAAA 
TGCTTTATGG GTGTGGCTCA AATCTTGTTG GAAGAACTTG ATCTATCCAG CATGGTGATT 
GGATGGTATA AATTGTTCCC TCCGTCCTCA CTGGTGGATC CCACTCTCGC TCCCCTGACC 
CGCCGGGCTT CCCAATCATC TCTGGAAAGT TCGTCCGGGC CTCCCTGCAT CCGGTCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017452164.1
CDS	76..3315
Translation	MQKGDLDYYWLDPATWHSRETSPISSHPVTWQPSKEGDRLIGRVILNKRTTMPKESGALLGLKVVGGKMTDLGRLGAFITKVKKGSLADVVGHLRAGDEVLEWNGKPLPGATNEEVYNIILESKSEPQVEIIVSRPIGDIPRIPESSHPPLESSSSSFESQKMERPSISVISPTSPGALKDAPQVLPGQLSVKLWYDKVGHQLIVNVLQATDLPPRVDGRPRNPYVKMYFLPDRSDKSKRRTKTVKKLLEPKWNQTFVYSHVHRRDFRERMLEITVWDQPRVQDEESEFLGEILIELETALLDDEPHWYKLQTHDESSLPLPQPSPFMPRRHIHGESSSKKLQRSQRISDSDISDYEVDDGIGVVPPVGYRASARESKATTLTVPEQQRTTHHRSRSVSPHRGDDQGRPRSRLPNVPLQRSLDEIHPTRRSRSPTRHHDASRSPADHRSRHVESQYSSEPDSELLMLPRAKRGRSAESLHMTSELQPSLDRARSASTNCLRPDTSLHSPERERGRWSPSLDRRRPASPRIQIQHASPENDRHSRKSERCSIQKQSRKGTASDADRVLPPCLSRRGYATPRATDQPVVRGKHPTRSRSSEHSSVRTLCSMHHLAPGGSAPPSPLLTRTHRQGSPTQSPPADTSFGSRRGRQLPQVPVRSGSIEQASLVVEERTRQMKMKVHRFKQTTGSGSSQELDHEQYSKKSRYIMEYNIHKDQYRSCDNASAKSSDSDVSDVSAISRASSTSRLSSTSFMSEQSERPRGRISSFTPKMQGRRMGTSGRAIIKSTSVSGEIYTLERNDGSQSDTAVGTVGAGGKKRRSSLSAKVVAIVSRRSRSTSQLSQTESGHKKLKSTIQRSTETGMAAEMRKMVRQPSRESTDGSINSYSSEGNLIFPGVRVGPDSQFSDFLDGLGPAQLVGRQTLATPAMGDIQIGMEDKKGQLEVEVIRARSLTQKPGSKSTPAPYVKVYLLENGACIAKKKTRIARKTLDPLYQQSLVFDESPQGKVLQVIVWGDYGRMDHKCFMGVAQILLEELDLSSMVIGWYKLFPPSSLVDPTLAPLTRRASQSSLESSSGPPCIRS

Target ORF information:

RefSeq Version	XM_017596675.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus regulating synaptic membrane exocytosis 1 (Rims1), transcript variant X22, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017596675.1

ORF Insert Sequence:

ATGCAAAAGG GTGACTTGGA TTACTACTGG TTGGATCCCG CCACGTGGCA CAGCAGGGAA 
ACGTCGCCTA TCAGTTCGCA TCCTGTAACG TGGCAGCCGT CTAAAGAGGG AGATCGACTA 
ATCGGCCGTG TTATTCTTAA CAAAAGAACA ACCATGCCCA AAGAATCAGG TGCATTATTG 
GGTCTGAAGG TGGTTGGAGG AAAAATGACG GACTTAGGGC GCCTTGGTGC TTTCATCACC 
AAAGTAAAGA AGGGCAGCCT GGCAGACGTC GTCGGACACC TAAGAGCAGG GGACGAAGTC 
CTAGAGTGGA ATGGTAAACC CCTGCCGGGA GCAACAAACG AAGAAGTTTA CAACATTATC 
TTAGAATCAA AATCAGAACC TCAAGTTGAG ATTATTGTTT CAAGGCCTAT TGGTGACATC 
CCCAGGATCC CTGAGAGTTC CCATCCTCCC CTGGAGTCCA GTTCAAGTTC CTTTGAATCT 
CAGAAAATGG AAAGGCCTTC TATTTCTGTT ATTTCTCCAA CCAGCCCTGG AGCTCTGAAA 
GATGCCCCAC AAGTCTTACC AGGGCAACTC TCAGTGAAGC TATGGTATGA TAAAGTGGGG 
CACCAGCTGA TTGTAAATGT TCTACAAGCA ACAGATCTAC CCCCTAGAGT AGATGGCCGT 
CCCAGGAATC CCTATGTAAA AATGTATTTT CTTCCAGATA GAAGCGACAA AAGTAAAAGG 
AGAACCAAAA CAGTAAAGAA ACTTCTAGAG CCAAAATGGA ACCAGACATT TGTCTACTCA 
CACGTACATC GTAGAGATTT TCGAGAGCGA ATGTTAGAGA TTACCGTGTG GGACCAGCCG 
AGAGTACAGG ACGAAGAGAG TGAATTTCTT GGAGAGATCC TCATAGAGTT GGAAACAGCG 
CTTTTAGATG ATGAGCCCCA TTGGTATAAA CTCCAGACAC ATGACGAATC TTCACTACCT 
CTGCCTCAGC CATCACCGTT CATGCCCAGG CGGCATATTC ATGGAGAGAG CTCCAGCAAA 
AAGCTACAAA GATCTCAGCG AATCAGTGAT AGTGACATCT CAGATTATGA GGTTGATGAT 
GGTATTGGAG TAGTGCCTCC AGTGGGTTAT AGAGCTAGTG CTAGAGAGAG TAAAGCAACC 
ACGTTAACAG TGCCAGAGCA ACAAAGAACT ACACATCACC GCTCACGTTC CGTGTCTCCT 
CATCGCGGCG ATGATCAGGG AAGGCCTCGT TCACGTTTAC CAAATGTGCC ATTACAGAGG 
AGCTTAGATG AAATTCATCC AACACGAAGG TCACGTTCTC CAACCCGACA CCATGATGCC 
TCCCGAAGCC CGGCCGATCA CAGATCCAGA CATGTGGAAA GTCAATATTC GTCAGAGCCA 
GACAGTGAGC TTCTCATGCT GCCCAGAGCA AAACGAGGAC GAAGTGCAGA AAGCCTACAC 
ATGACCAGTG AACTGCAGCC CTCTCTTGAC AGGGCTAGGA GTGCTAGTAC CAACTGCTTG 
AGACCAGATA CTAGTTTGCA TTCACCAGAA CGAGAAAGGG GTAGATGGTC CCCCTCCCTA 
GATAGGAGGC GACCTGCTAG CCCCAGGATT CAAATCCAGC ATGCATCTCC GGAGAATGAC 
AGGCACTCCA GAAAGTCTGA AAGATGTAGC ATCCAAAAAC AGTCTAGGAA AGGCACAGCC 
TCTGATGCAG ACAGGGTTCT CCCACCATGC CTTTCTAGAA GGGGATACGC AACCCCAAGA 
GCAACCGATC AACCGGTCGT TAGGGGAAAG CATCCCACTC GTTCACGGTC GAGCGAGCAC 
TCTAGTGTCA GAACCCTGTG TTCTATGCAC CACCTTGCCC CCGGAGGGTC GGCGCCACCT 
TCTCCACTTC TGACAAGAAC GCACCGACAA GGAAGCCCAA CCCAGTCTCC TCCAGCAGAC 
ACATCCTTCG GCAGTCGCCG TGGAAGACAG CTCCCACAGG TGCCAGTTCG AAGCGGCAGT 
ATAGAACAAG CAAGCTTAGT AGTGGAGGAG CGAACGAGAC AGATGAAAAT GAAAGTTCAC 
CGATTTAAGC AGACAACAGG GTCTGGGTCT AGTCAAGAAC TTGACCACGA GCAATACTCC 
AAGAAGTCAC GTTACATTAT GGAGTACAAC ATACATAAAG ATCAGTACAG AAGCTGTGAT 
AACGCGTCTG CCAAGTCTTC AGATAGTGAT GTCAGTGATG TGTCCGCCAT TTCCAGAGCC 
AGCAGTACCT CACGCCTCAG CAGCACAAGC TTTATGTCAG AGCAGTCTGA GCGCCCCAGG 
GGTAGGATCA GTTCATTTAC CCCCAAAATG CAAGGCAGAC GGATGGGGAC TTCAGGAAGA 
GCCATCATCA AGAGCACCAG TGTAAGTGGA GAGATATATA CACTGGAACG TAATGACGGT 
AGCCAGTCGG ACACGGCCGT GGGTACCGTC GGAGCCGGTG GAAAGAAACG AAGATCCAGC 
CTGAGCGCCA AAGTGGTAGC CATTGTGTCT CGAAGAAGCA GGAGCACGTC ACAGCTCAGC 
CAGACAGAGT CGGGCCACAA GAAGTTGAAA AGCACCATCC AGAGGAGTAC GGAAACAGGA 
ATGGCAGCTG AAATGCGGAA GATGGTGAGA CAGCCGAGCC GGGAGTCCAC GGATGGCAGC 
ATCAACAGTT ATAGCTCGGA AGGAAACTTG ATATTTCCTG GAGTTCGAGT AGGACCCGAC 
AGTCAGTTCA GTGATTTCCT TGATGGGTTG GGACCAGCGC AGCTCGTTGG CCGTCAGACG 
CTCGCCACCC CGGCCATGGG CGATATCCAA ATCGGGATGG AGGATAAGAA GGGTCAGTTG 
GAGGTTGAGG TTATCAGAGC CCGGAGCCTT ACACAAAAAC CTGGTTCCAA ATCTACACCC 
GCTCCCTATG TGAAAGTATA TCTTTTGGAA AATGGAGCCT GTATTGCCAA AAAGAAGACA 
AGAATTGCAC GGAAAACTCT CGATCCTTTG TATCAGCAGT CCCTGGTTTT TGATGAAAGT 
CCACAGGGTA AAGTTCTTCA GGTGATTGTC TGGGGTGACT ATGGAAGAAT GGACCACAAA 
TGCTTTATGG GTGTGGCTCA AATCTTGTTG GAAGAACTTG ATCTATCCAG CATGGTGATT 
GGATGGTATA AATTGTTCCC TCCGTCCTCA CTGGTGGATC CCACTCTCGC TCCCCTGACC 
CGCCGGGCTT CCCAATCATC TCTGGAAAGT TCGTCCGGGC CTCCCTGCAT CCGGTCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rims1 ( XM_017596676.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017452165.1 Rattus norvegicus regulating synaptic membrane exocytosis 1 (Rims1), transcript variant X23, mRNA.

Price & Availability↑

CloneID	ORa62893
Clone ID Related Accession (Same CDS sequence)	XM_017596676.1
Accession Version	XM_017596676.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3087bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	regulating synaptic membrane exocytosis protein 1 isoform X23
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005108.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCAAAG AATCAGGTGC ATTATTGGGT CTGAAGGTGG TTGGAGGAAA AATGACGGAC 
TTAGGGCGCC TTGGTGCTTT CATCACCAAA GTAAAGAAGG GCAGCCTGGC AGACGTCGTC 
GGACACCTAA GAGCAGGGGA CGAAGTCCTA GAGTGGAATG GTAAACCCCT GCCGGGAGCA 
ACAAACGAAG AAGTTTACAA CATTATCTTA GAATCAAAAT CAGAACCTCA AGTTGAGATT 
ATTGTTTCAA GGCCTATTGG TGACATCCCC AGGATCCCTG AGAGTTCCCA TCCTCCCCTG 
GAGTCCAGTT CAAGTTCCTT TGAATCTCAG AAAATGGAAA GGCCTTCTAT TTCTGTTATT 
TCTCCAACCA GCCCTGGAGC TCTGAAAGAT GCCCCACAAG TCTTACCAGG GCAACTCTCA 
GTGAAGCTAT GGTATGATAA AGTGGGGCAC CAGCTGATTG TAAATGTTCT ACAAGCAACA 
GATCTACCCC CTAGAGTAGA TGGCCGTCCC AGGAATCCCT ATGTAAAAAT GTATTTTCTT 
CCAGATAGAA GCGACAAAAG TAAAAGGAGA ACCAAAACAG TAAAGAAACT TCTAGAGCCA 
AAATGGAACC AGACATTTGT CTACTCACAC GTACATCGTA GAGATTTTCG AGAGCGAATG 
TTAGAGATTA CCGTGTGGGA CCAGCCGAGA GTACAGGACG AAGAGAGTGA ATTTCTTGGA 
GAGATCCTCA TAGAGTTGGA AACAGCGCTT TTAGATGATG AGCCCCATTG GTATAAACTC 
CAGACACATG ACGAATCTTC ACTACCTCTG CCTCAGCCAT CACCGTTCAT GCCCAGGCGG 
CATATTCATG GAGAGAGCTC CAGCAAAAAG CTACAAAGAT CTCAGCGAAT CAGTGATAGT 
GACATCTCAG ATTATGAGGT TGATGATGGT ATTGGAGTAG TGCCTCCAGT GGGTTATAGA 
GCTAGTGCTA GAGAGAGTAA AGCAACCACG TTAACAGTGC CAGAGCAACA AAGAACTACA 
CATCACCGCT CACGTTCCGT GTCTCCTCAT CGCGGCGATG ATCAGGGAAG GCCTCGTTCA 
CGTTTACCAA ATGTGCCATT ACAGAGGAGC TTAGATGAAA TTCATCCAAC ACGAAGGTCA 
CGTTCTCCAA CCCGACACCA TGATGCCTCC CGAAGCCCGG CCGATCACAG ATCCAGACAT 
GTGGAAAGTC AATATTCGTC AGAGCCAGAC AGTGAGCTTC TCATGCTGCC CAGAGCAAAA 
CGAGGACGAA GTGCAGAAAG CCTACACATG ACCAGTGAAC TGCAGCCCTC TCTTGACAGG 
GCTAGGAGTG CTAGTACCAA CTGCTTGAGA CCAGATACTA GTTTGCATTC ACCAGAACGA 
GAAAGGGGTA GATGGTCCCC CTCCCTAGAT AGGAGGCGAC CTGCTAGCCC CAGGATTCAA 
ATCCAGCATG CATCTCCGGA GAATGACAGG CACTCCAGAA AGTCTGAAAG ATGTAGCATC 
CAAAAACAGT CTAGGAAAGG CACAGCCTCT GATGCAGACA GGGTTCTCCC ACCATGCCTT 
TCTAGAAGGG GATACGCAAC CCCAAGAGCA ACCGATCAAC CGGTCGTTAG GGGAAAGCAT 
CCCACTCGTT CACGGTCGAG CGAGCACTCT AGTGTCAGAA CCCTGTGTTC TATGCACCAC 
CTTGCCCCCG GAGGGTCGGC GCCACCTTCT CCACTTCTGA CAAGAACGCA CCGACAAGGA 
AGCCCAACCC AGTCTCCTCC AGCAGACACA TCCTTCGGCA GTCGCCGTGG AAGACAGCTC 
CCACAGGTGC CAGTTCGAAG CGGCAGTATA GAACAAGCAA GCTTAGTAGT GGAGGAGCGA 
ACGAGACAGA TGAAAATGAA AGTTCACCGA TTTAAGCAGA CAACAGGGTC TGGGTCTAGT 
CAAGAACTTG ACCACGAGCA ATACTCCAAG AAGTCACGTT ACATTATGGA GTACAACATA 
CATAAAGATC AGTACAGAAG CTGTGATAAC GCGTCTGCCA AGTCTTCAGA TAGTGATGTC 
AGTGATGTGT CCGCCATTTC CAGAGCCAGC AGTACCTCAC GCCTCAGCAG CACAAGCTTT 
ATGTCAGAGC AGTCTGAGCG CCCCAGGGGT AGGATCAGTT CATTTACCCC CAAAATGCAA 
GGCAGACGGA TGGGGACTTC AGGAAGAGCC ATCATCAAGA GCACCAGTGT AAGTGGAGAG 
ATATATACAC TGGAACGTAA TGACGGTAGC CAGTCGGACA CGGCCGTGGG TACCGTCGGA 
GCCGGTGGAA AGAAACGAAG ATCCAGCCTG AGCGCCAAAG TGGTAGCCAT TGTGTCTCGA 
AGAAGCAGGA GCACGTCACA GCTCAGCCAG ACAGAGTCGG GCCACAAGAA GTTGAAAAGC 
ACCATCCAGA GGAGTACGGA AACAGGAATG GCAGCTGAAA TGCGGAAGAT GGTGAGACAG 
CCGAGCCGGG AGTCCACGGA TGGCAGCATC AACAGTTATA GCTCGGAAGG AAACTTGATA 
TTTCCTGGAG TTCGAGTAGG ACCCGACAGT CAGTTCAGTG ATTTCCTTGA TGGGTTGGGA 
CCAGCGCAGC TCGTTGGCCG TCAGACGCTC GCCACCCCGG CCATGGGCGA TATCCAAATC 
GGGATGGAGG ATAAGAAGGG TCAGTTGGAG GTTGAGGTTA TCAGAGCCCG GAGCCTTACA 
CAAAAACCTG GTTCCAAATC TACACCCGCT CCCTATGTGA AAGTATATCT TTTGGAAAAT 
GGAGCCTGTA TTGCCAAAAA GAAGACAAGA ATTGCACGGA AAACTCTCGA TCCTTTGTAT 
CAGCAGTCCC TGGTTTTTGA TGAAAGTCCA CAGGGTAAAG TTCTTCAGGT GATTGTCTGG 
GGTGACTATG GAAGAATGGA CCACAAATGC TTTATGGGTG TGGCTCAAAT CTTGTTGGAA 
GAACTTGATC TATCCAGCAT GGTGATTGGA TGGTATAAAT TGTTCCCTCC GTCCTCACTG 
GTGGATCCCA CTCTCGCTCC CCTGACCCGC CGGGCTTCCC AATCATCTCT GGAAAGTTCG 
TCCGGGCCTC CCTGCATCCG GTCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017452165.1
CDS	826..3912
Translation	MPKESGALLGLKVVGGKMTDLGRLGAFITKVKKGSLADVVGHLRAGDEVLEWNGKPLPGATNEEVYNIILESKSEPQVEIIVSRPIGDIPRIPESSHPPLESSSSSFESQKMERPSISVISPTSPGALKDAPQVLPGQLSVKLWYDKVGHQLIVNVLQATDLPPRVDGRPRNPYVKMYFLPDRSDKSKRRTKTVKKLLEPKWNQTFVYSHVHRRDFRERMLEITVWDQPRVQDEESEFLGEILIELETALLDDEPHWYKLQTHDESSLPLPQPSPFMPRRHIHGESSSKKLQRSQRISDSDISDYEVDDGIGVVPPVGYRASARESKATTLTVPEQQRTTHHRSRSVSPHRGDDQGRPRSRLPNVPLQRSLDEIHPTRRSRSPTRHHDASRSPADHRSRHVESQYSSEPDSELLMLPRAKRGRSAESLHMTSELQPSLDRARSASTNCLRPDTSLHSPERERGRWSPSLDRRRPASPRIQIQHASPENDRHSRKSERCSIQKQSRKGTASDADRVLPPCLSRRGYATPRATDQPVVRGKHPTRSRSSEHSSVRTLCSMHHLAPGGSAPPSPLLTRTHRQGSPTQSPPADTSFGSRRGRQLPQVPVRSGSIEQASLVVEERTRQMKMKVHRFKQTTGSGSSQELDHEQYSKKSRYIMEYNIHKDQYRSCDNASAKSSDSDVSDVSAISRASSTSRLSSTSFMSEQSERPRGRISSFTPKMQGRRMGTSGRAIIKSTSVSGEIYTLERNDGSQSDTAVGTVGAGGKKRRSSLSAKVVAIVSRRSRSTSQLSQTESGHKKLKSTIQRSTETGMAAEMRKMVRQPSRESTDGSINSYSSEGNLIFPGVRVGPDSQFSDFLDGLGPAQLVGRQTLATPAMGDIQIGMEDKKGQLEVEVIRARSLTQKPGSKSTPAPYVKVYLLENGACIAKKKTRIARKTLDPLYQQSLVFDESPQGKVLQVIVWGDYGRMDHKCFMGVAQILLEELDLSSMVIGWYKLFPPSSLVDPTLAPLTRRASQSSLESSSGPPCIRS

Target ORF information:

RefSeq Version	XM_017596676.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus regulating synaptic membrane exocytosis 1 (Rims1), transcript variant X23, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017596676.1

ORF Insert Sequence:

ATGCCCAAAG AATCAGGTGC ATTATTGGGT CTGAAGGTGG TTGGAGGAAA AATGACGGAC 
TTAGGGCGCC TTGGTGCTTT CATCACCAAA GTAAAGAAGG GCAGCCTGGC AGACGTCGTC 
GGACACCTAA GAGCAGGGGA CGAAGTCCTA GAGTGGAATG GTAAACCCCT GCCGGGAGCA 
ACAAACGAAG AAGTTTACAA CATTATCTTA GAATCAAAAT CAGAACCTCA AGTTGAGATT 
ATTGTTTCAA GGCCTATTGG TGACATCCCC AGGATCCCTG AGAGTTCCCA TCCTCCCCTG 
GAGTCCAGTT CAAGTTCCTT TGAATCTCAG AAAATGGAAA GGCCTTCTAT TTCTGTTATT 
TCTCCAACCA GCCCTGGAGC TCTGAAAGAT GCCCCACAAG TCTTACCAGG GCAACTCTCA 
GTGAAGCTAT GGTATGATAA AGTGGGGCAC CAGCTGATTG TAAATGTTCT ACAAGCAACA 
GATCTACCCC CTAGAGTAGA TGGCCGTCCC AGGAATCCCT ATGTAAAAAT GTATTTTCTT 
CCAGATAGAA GCGACAAAAG TAAAAGGAGA ACCAAAACAG TAAAGAAACT TCTAGAGCCA 
AAATGGAACC AGACATTTGT CTACTCACAC GTACATCGTA GAGATTTTCG AGAGCGAATG 
TTAGAGATTA CCGTGTGGGA CCAGCCGAGA GTACAGGACG AAGAGAGTGA ATTTCTTGGA 
GAGATCCTCA TAGAGTTGGA AACAGCGCTT TTAGATGATG AGCCCCATTG GTATAAACTC 
CAGACACATG ACGAATCTTC ACTACCTCTG CCTCAGCCAT CACCGTTCAT GCCCAGGCGG 
CATATTCATG GAGAGAGCTC CAGCAAAAAG CTACAAAGAT CTCAGCGAAT CAGTGATAGT 
GACATCTCAG ATTATGAGGT TGATGATGGT ATTGGAGTAG TGCCTCCAGT GGGTTATAGA 
GCTAGTGCTA GAGAGAGTAA AGCAACCACG TTAACAGTGC CAGAGCAACA AAGAACTACA 
CATCACCGCT CACGTTCCGT GTCTCCTCAT CGCGGCGATG ATCAGGGAAG GCCTCGTTCA 
CGTTTACCAA ATGTGCCATT ACAGAGGAGC TTAGATGAAA TTCATCCAAC ACGAAGGTCA 
CGTTCTCCAA CCCGACACCA TGATGCCTCC CGAAGCCCGG CCGATCACAG ATCCAGACAT 
GTGGAAAGTC AATATTCGTC AGAGCCAGAC AGTGAGCTTC TCATGCTGCC CAGAGCAAAA 
CGAGGACGAA GTGCAGAAAG CCTACACATG ACCAGTGAAC TGCAGCCCTC TCTTGACAGG 
GCTAGGAGTG CTAGTACCAA CTGCTTGAGA CCAGATACTA GTTTGCATTC ACCAGAACGA 
GAAAGGGGTA GATGGTCCCC CTCCCTAGAT AGGAGGCGAC CTGCTAGCCC CAGGATTCAA 
ATCCAGCATG CATCTCCGGA GAATGACAGG CACTCCAGAA AGTCTGAAAG ATGTAGCATC 
CAAAAACAGT CTAGGAAAGG CACAGCCTCT GATGCAGACA GGGTTCTCCC ACCATGCCTT 
TCTAGAAGGG GATACGCAAC CCCAAGAGCA ACCGATCAAC CGGTCGTTAG GGGAAAGCAT 
CCCACTCGTT CACGGTCGAG CGAGCACTCT AGTGTCAGAA CCCTGTGTTC TATGCACCAC 
CTTGCCCCCG GAGGGTCGGC GCCACCTTCT CCACTTCTGA CAAGAACGCA CCGACAAGGA 
AGCCCAACCC AGTCTCCTCC AGCAGACACA TCCTTCGGCA GTCGCCGTGG AAGACAGCTC 
CCACAGGTGC CAGTTCGAAG CGGCAGTATA GAACAAGCAA GCTTAGTAGT GGAGGAGCGA 
ACGAGACAGA TGAAAATGAA AGTTCACCGA TTTAAGCAGA CAACAGGGTC TGGGTCTAGT 
CAAGAACTTG ACCACGAGCA ATACTCCAAG AAGTCACGTT ACATTATGGA GTACAACATA 
CATAAAGATC AGTACAGAAG CTGTGATAAC GCGTCTGCCA AGTCTTCAGA TAGTGATGTC 
AGTGATGTGT CCGCCATTTC CAGAGCCAGC AGTACCTCAC GCCTCAGCAG CACAAGCTTT 
ATGTCAGAGC AGTCTGAGCG CCCCAGGGGT AGGATCAGTT CATTTACCCC CAAAATGCAA 
GGCAGACGGA TGGGGACTTC AGGAAGAGCC ATCATCAAGA GCACCAGTGT AAGTGGAGAG 
ATATATACAC TGGAACGTAA TGACGGTAGC CAGTCGGACA CGGCCGTGGG TACCGTCGGA 
GCCGGTGGAA AGAAACGAAG ATCCAGCCTG AGCGCCAAAG TGGTAGCCAT TGTGTCTCGA 
AGAAGCAGGA GCACGTCACA GCTCAGCCAG ACAGAGTCGG GCCACAAGAA GTTGAAAAGC 
ACCATCCAGA GGAGTACGGA AACAGGAATG GCAGCTGAAA TGCGGAAGAT GGTGAGACAG 
CCGAGCCGGG AGTCCACGGA TGGCAGCATC AACAGTTATA GCTCGGAAGG AAACTTGATA 
TTTCCTGGAG TTCGAGTAGG ACCCGACAGT CAGTTCAGTG ATTTCCTTGA TGGGTTGGGA 
CCAGCGCAGC TCGTTGGCCG TCAGACGCTC GCCACCCCGG CCATGGGCGA TATCCAAATC 
GGGATGGAGG ATAAGAAGGG TCAGTTGGAG GTTGAGGTTA TCAGAGCCCG GAGCCTTACA 
CAAAAACCTG GTTCCAAATC TACACCCGCT CCCTATGTGA AAGTATATCT TTTGGAAAAT 
GGAGCCTGTA TTGCCAAAAA GAAGACAAGA ATTGCACGGA AAACTCTCGA TCCTTTGTAT 
CAGCAGTCCC TGGTTTTTGA TGAAAGTCCA CAGGGTAAAG TTCTTCAGGT GATTGTCTGG 
GGTGACTATG GAAGAATGGA CCACAAATGC TTTATGGGTG TGGCTCAAAT CTTGTTGGAA 
GAACTTGATC TATCCAGCAT GGTGATTGGA TGGTATAAAT TGTTCCCTCC GTCCTCACTG 
GTGGATCCCA CTCTCGCTCC CCTGACCCGC CGGGCTTCCC AATCATCTCT GGAAAGTTCG 
TCCGGGCCTC CCTGCATCCG GTCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rims1 ( NM_052829.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_439894.1 Rattus norvegicus regulating synaptic membrane exocytosis 1 (Rims1), mRNA.

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CloneID	ORa09170
Clone ID Related Accession (Same CDS sequence)	NM_052829.1
Accession Version	NM_052829.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4662bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-20
Organism	Rattus norvegicus(Norway rat)
Product	regulating synaptic membrane exocytosis protein 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF007836.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF007836.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGTCCTCGG CCGTGGGGCC CCGAGGTCCT CGCCCACCCA CGGTGCCTCC CCCTATGCAA 
GAACTGCCCG ACCTGAGCCA CCTGACCGAG GAGGAGAGGA ACATTATCAT GGCAGTGATG 
GACCGGCAGA AGGAAGAGGA GGAAAAAGAA GAGGCCATGC TCAAGTGTGT TGTCAGGGAC 
ATGGCGAAGC CTGCTGCCTG CAAAACACCA AGAAATGCTG AAAGCCAGCC CCATCAACCA 
CCACTGAACA TTTTCAGATG TGTCTGTGTT CCCAGAAAGC CAAGCAGCGA AGAGGGAGGC 
CCAGAAAGAG ACTGGAGATT GCATCAACAG TTTGAAAGCT ACAAGGAGCA AGTGAGAAAA 
ATCGGAGAGG AAGCGAGGCG TTACCAGGGC GAGCACAAGG ATGATGCCCC GACGTGTGGA 
ATCTGTCATA AGACAAAGTT TGCTGATGGA TGTGGCCATC TCTGCTCCTA TTGTCGCACC 
AAGTTCTGTG CACGCTGCGG AGGCCGTGTG TCTCTGCGAT CGAACAATGA GGACAAAGTG 
GTTATGTGGG TATGCAATTT ATGTCGAAAG CAACAAGAAA TCTTAACGAA ATCTGGAGCG 
TGGTTCTTTG GAAGTGGCCC TCAGCAGCCT AGTCAAGATG GGACTCTGAG TGACACGGCC 
ACAGGTGCTG GATCTGAGGT GCCAAGAGAA AAGAAAGCAA GGCTCCAAGA GCGATCAAGG 
TCTCAGACGC CCTTGAGTAC AGCAGCTGTC TCTTCCCAAG ACACTGCTAC CCCCGGTGCA 
CCGTTGCACA GGAACAAAGG GGCTGAGCCC TCACAGCAAG CCTTGGGTCC TGAACAGAAG 
CAGGCATCAA GATCAAGAAG CGAGCCACCG AGGGAAAGGA AGAAGGCTCC AGGGCTTTCA 
GAGCAGAATG GCAAGGGAGG CCAGAAGAGC GAGCGCAAAC GTGTCCCCAA GTCTGTGGTG 
CAACCCGGGG AAGGGATCGC GGATGAGAGG GAGAGGAAAG AGAGGCGGGA AACCCGCAGG 
TTGGAGAAAG GGCGCTCCCA GGACTACTCA GACCGGCCTG AGAAACGCGA CAATGGCAGG 
GTGGCGGAAG ACCAGAAGCA GAGGAAGGAG GAGGAGTACC AGACTAGGTA CCGCAGCGAC 
CCTAACCTGG CTCGCTACCC GGTGAAGGCG CCGCCAGAGG AGCAGCAGAT GCGCATGCAC 
GCCCGGGTGT CCCGAGCGAG GCACGAGCGG CGCCACAGCG ACGTGGCGCT CCCGCACACC 
GAGGCAGCTG CCGCCGCGCC GGCTGAGGCC ACGGCGGGCA AGCGCGCGCC GGCCACCGCC 
AGGGTCTCTC CCCCGGAGTC CCCGCGCGCA CGCGCGGCGG CCGCCCAGCC TCCCACCGAG 
CACGGGCCAC CGCCGCCGCG GCCAGCCCCG GGTCCCGCAG AGCCACCCGA GCCGCGCGTC 
CCCGAGCCGC TCCGTAAGCA GGGCCGCCTG GACCCGGGCT CGGCCGTGCT TCTGCGCAAG 
GCCAAGCGCG AGAAGGCGGA GAGCATGCTG CGGAACGACT CGCTGAGCTC CGATCAGTCC 
GAGTCCGTGC GGCCATCCCC GCCCAAGCCT CACCGGCCCA AGCGGGGAGG CAAGAGACGT 
CAGATGTCGG TGAGCAGCTC GGAGGAGGAG GGCGTGTCCA CACCGGAGTA CACGAGCTGC 
GAGGACGTGG AGCTGGAGAG CGAGAGCGTG AGCGAGAAAG GTGACTTGGA TTACTACTGG 
TTGGATCCCG CCACGTGGCA CAGCAGGGAA ACGTCGCCTA TCAGTTCGCA TCCTGTAACG 
TGGCAGCCGT CTAAAGAGGG AGATCGACTA ATCGGCCGTG TTATTCTTAA CAAAAGAACA 
ACCATGCCCA AAGAATCAGG TGCATTATTG GGTCTGAAGG TGGTTGGAGG AAAAATGACG 
GACTTAGGGC GCCTTGGTGC TTTCATCACC AAAGTAAAGA AGGGCAGCCT GGCAGACGTC 
GTCGGACACC TAAGAGCAGG GGACGAAGTC CTAGAGTGGA ATGGTAAACC CCTGCCGGGA 
GCAACAAACG AAGAAGTTTA CAACATTATC TTAGAATCAA AATCAGAACC TCAAGTTGAG 
ATTATTGTTT CAAGGCCTAT TGGTGACATC CCCAGGATCC CTGAGAGTTC CCATCCTCCC 
CTGGAGTCCA GTTCAAGTTC CTTTGAATCT CAGAAAATGG AAAGGCCTTC TATTTCTGTT 
ATTTCTCCAA CCAGCCCTGG AGCTCTGAAA GATGCCCCAC AAGTCTTACC AGGGCAACTC 
TCAGTGAAGC TATGGTATGA TAAAGTGGGG CACCAGCTGA TTGTAAATGT TCTACAAGCA 
ACAGATCTAC CCCCTAGAGT AGATGGCCGT CCCAGGAATC CCTATGTAAA AATGTATTTT 
CTTCCAGATA GAAGCGACAA AAGTAAAAGG AGAACCAAAA CAGTAAAGAA ACTTCTAGAG 
CCAAAATGGA ACCAGACATT TGTCTACTCA CACGTACATC GTAGAGATTT TCGAGAGCGA 
ATGTTAGAGA TTACCGTGTG GGACCAGCCG AGAGTACAGG ACGAAGAGAG TGAATTTCTT 
GGAGAGATCC TCATAGAGTT GGAAACAGCG CTTTTAGATG ATGAGCCCCA TTGGTATAAA 
CTCCAGACAC ATGACGAATC TTCACTACCT CTGCCTCAGC CATCACCGTT CATGCCCAGG 
CGGCATATTC ATGGAGAGAG CTCCAGCAAA AAGCTACAAA GATCTCAGCG AATCAGTGAT 
AGTGACATCT CAGATTATGA GGTTGATGAT GGTATTGGAG TAGTGCCTCC AGTGGGTTAT 
AGAGCTAGTG CTAGAGAGAG TAAAGCAACC ACGTTAACAG TGCCAGAGCA ACAAAGAACT 
ACACATCACC GCTCACGTTC CGTGTCTCCT CATCGCGGCG ATGATCAGGG AAGGCCTCGT 
TCACGTTTAC CAAATGTGCC ATTACAGAGG AGCTTAGATG AAATTCATCC AACACGAAGG 
TCACGTTCTC CAACCCGACA CCATGATGCC TCCCGAAGCC CGGCCGATCA CAGATCCAGA 
CATGTGGAAA GTCAATATTC GTCAGAGCCA GACAGTGAGC TTCTCATGCT GCCCAGAGCA 
AAACGAGGAC GAAGTGCAGA AAGCCTACAC ATGACCAGTG AACTGCAGCC CTCTCTTGAC 
AGGGCTAGGA GTGCTAGTAC CAACTGCTTG AGACCAGATA CTAGTTTGCA TTCACCAGAA 
CGAGAAAGGC ACTCCAGAAA GTCTGAAAGA TGTAGCATCC AAAAACAGTC TAGGAAAGGC 
ACAGCCTCTG ATGCAGACAG AACGCACCGA CAAGGAAGCC CAACCCAGTC TCCTCCAGCA 
GACACATCCT TCGGCAGTCG CCGTGGAAGA CAGCTCCCAC AGGTGCCAGT TCGAAGCGGC 
AGTATAGAAC AAGCAAGCTT AGTAGTGGAG GAGCGAACGA GACAGATGAA AGTGAAAGTT 
CACCGATTTA AGCAGACAAC AGGGTCTGGG TCTAGTCAAG AACTTGACCA CGAGCAATAC 
TCCAAGTACA ACATACATAA AGATCAGTAC AGAAGCTGTG ATAACGCGTC TGCCAAGTCT 
TCAGATAGTG ATGTCAGTGA TGTGTCCGCC ATTTCCAGAG CCAGCAGTAC CTCACGCCTC 
AGCAGCACAA GCTTTATGTC AGAGCAGTCT GAGCGCCCCA GGGGTAGGAT CAGTTCATTT 
ACCCCCAAAA TGCAAGGCAG ACGGATGGGG ACTTCAGGAA GAGCCATCAT CAAGAGCACC 
AGTGTAAGTG GAGAGATATA TACACTGGAA CGTAATGACG GTAGCCAGTC GGACACGGCC 
GTAGGTACCG TCGGAGCCGG TGGAAAGAAA CGAAGATCCA GCCTGAGCGC CAAAGTGGTA 
GCCATTGTGT CTCGAAGAAG CAGGAGCACG TCACAGCTCA GCCAGACAGA GTCGGGCCAC 
AAGAAGTTGA AAAGCACCAT CCAGAGGAGT ACGGAAACAG GAATGGCAGC TGAAATGCGG 
AAGATGGTGA GACAGCCGAG CCGGGAGTCC ACGGATGGCA GCATCAACAG TTATAGCTCG 
GAAGGAAACT TGATATTTCC TGGAGTTCGA GTAGGACCCG ACAGTCAGTT CAGTGATTTC 
CTTGATGGGT TGGGACCAGC GCAGCTCGTT GGCCGTCAGA CGCTCGCCAC CCCGGCCATG 
GGCGATATCC AAATCGGGAT GGAGGATAAG AAGGGTCAGT TGGAGGTTGA GGTTATCAGA 
GCCCGGAGCC TTACACAAAA ACCTGGTTCC AAATCTACAC CCGCTCCCTA TGTGAAAGTA 
TATCTTTTGG AAAATGGAGC CTGTATTGCC AAAAAGAAGA CAAGAATTGC ACGGAAAACT 
CTCGATCCTT TGTATCAGCA GTCCCTGGTT TTTGATGAAA GTCCACAGGG TAAAGTTCTT 
CAGGTGATTG TCTGGGGTGA CTATGGAAGA ATGGACCACA AATGCTTTAT GGGTGTGGCT 
CAAATCTTGT TGGAAGAACT TGATCTATCC AGCATGGTGA TTGGATGGTA TAAATTGTTC 
CCTCCGTCCT CACTGGTGGA TCCCACTCTC GCTCCCCTGA CCCGCCGGGC TTCCCAATCA 
TCTCTGGAAA GTTCGTCCGG GCCTCCCTGC ATCCGGTCAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_439894.1
CDS	415..5076
Translation	MSSAVGPRGPRPPTVPPPMQELPDLSHLTEEERNIIMAVMDRQKEEEEKEEAMLKCVVRDMAKPAACKTPRNAESQPHQPPLNIFRCVCVPRKPSSEEGGPERDWRLHQQFESYKEQVRKIGEEARRYQGEHKDDAPTCGICHKTKFADGCGHLCSYCRTKFCARCGGRVSLRSNNEDKVVMWVCNLCRKQQEILTKSGAWFFGSGPQQPSQDGTLSDTATGAGSEVPREKKARLQERSRSQTPLSTAAVSSQDTATPGAPLHRNKGAEPSQQALGPEQKQASRSRSEPPRERKKAPGLSEQNGKGGQKSERKRVPKSVVQPGEGIADERERKERRETRRLEKGRSQDYSDRPEKRDNGRVAEDQKQRKEEEYQTRYRSDPNLARYPVKAPPEEQQMRMHARVSRARHERRHSDVALPHTEAAAAAPAEATAGKRAPATARVSPPESPRARAAAAQPPTEHGPPPPRPAPGPAEPPEPRVPEPLRKQGRLDPGSAVLLRKAKREKAESMLRNDSLSSDQSESVRPSPPKPHRPKRGGKRRQMSVSSSEEEGVSTPEYTSCEDVELESESVSEKGDLDYYWLDPATWHSRETSPISSHPVTWQPSKEGDRLIGRVILNKRTTMPKESGALLGLKVVGGKMTDLGRLGAFITKVKKGSLADVVGHLRAGDEVLEWNGKPLPGATNEEVYNIILESKSEPQVEIIVSRPIGDIPRIPESSHPPLESSSSSFESQKMERPSISVISPTSPGALKDAPQVLPGQLSVKLWYDKVGHQLIVNVLQATDLPPRVDGRPRNPYVKMYFLPDRSDKSKRRTKTVKKLLEPKWNQTFVYSHVHRRDFRERMLEITVWDQPRVQDEESEFLGEILIELETALLDDEPHWYKLQTHDESSLPLPQPSPFMPRRHIHGESSSKKLQRSQRISDSDISDYEVDDGIGVVPPVGYRASARESKATTLTVPEQQRTTHHRSRSVSPHRGDDQGRPRSRLPNVPLQRSLDEIHPTRRSRSPTRHHDASRSPADHRSRHVESQYSSEPDSELLMLPRAKRGRSAESLHMTSELQPSLDRARSASTNCLRPDTSLHSPERERHSRKSERCSIQKQSRKGTASDADRTHRQGSPTQSPPADTSFGSRRGRQLPQVPVRSGSIEQASLVVEERTRQMKVKVHRFKQTTGSGSSQELDHEQYSKYNIHKDQYRSCDNASAKSSDSDVSDVSAISRASSTSRLSSTSFMSEQSERPRGRISSFTPKMQGRRMGTSGRAIIKSTSVSGEIYTLERNDGSQSDTAVGTVGAGGKKRRSSLSAKVVAIVSRRSRSTSQLSQTESGHKKLKSTIQRSTETGMAAEMRKMVRQPSRESTDGSINSYSSEGNLIFPGVRVGPDSQFSDFLDGLGPAQLVGRQTLATPAMGDIQIGMEDKKGQLEVEVIRARSLTQKPGSKSTPAPYVKVYLLENGACIAKKKTRIARKTLDPLYQQSLVFDESPQGKVLQVIVWGDYGRMDHKCFMGVAQILLEELDLSSMVIGWYKLFPPSSLVDPTLAPLTRRASQSSLESSSGPPCIRS

Target ORF information:

RefSeq Version	NM_052829.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus regulating synaptic membrane exocytosis 1 (Rims1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_052829.1

ORF Insert Sequence:

ATGTCCTCGG CCGTGGGGCC CCGAGGTCCT CGCCCACCCA CGGTGCCTCC CCCTATGCAA 
GAACTGCCCG ACCTGAGCCA CCTGACCGAG GAGGAGAGGA ACATTATCAT GGCAGTGATG 
GACCGGCAGA AGGAAGAGGA GGAAAAAGAA GAGGCCATGC TCAAGTGTGT TGTCAGGGAC 
ATGGCGAAGC CTGCTGCCTG CAAAACACCA AGAAATGCTG AAAGCCAGCC CCATCAACCA 
CCACTGAACA TTTTCAGATG TGTCTGTGTT CCCAGAAAGC CAAGCAGCGA AGAGGGAGGC 
CCAGAAAGAG ACTGGAGATT GCATCAACAG TTTGAAAGCT ACAAGGAGCA AGTGAGAAAA 
ATCGGAGAGG AAGCGAGGCG TTACCAGGGC GAGCACAAGG ATGATGCCCC GACGTGTGGA 
ATCTGTCATA AGACAAAGTT TGCTGATGGA TGTGGCCATC TCTGCTCCTA TTGTCGCACC 
AAGTTCTGTG CACGCTGCGG AGGCCGTGTG TCTCTGCGAT CGAACAATGA GGACAAAGTG 
GTTATGTGGG TATGCAATTT ATGTCGAAAG CAACAAGAAA TCTTAACGAA ATCTGGAGCG 
TGGTTCTTTG GAAGTGGCCC TCAGCAGCCT AGTCAAGATG GGACTCTGAG TGACACGGCC 
ACAGGTGCTG GATCTGAGGT GCCAAGAGAA AAGAAAGCAA GGCTCCAAGA GCGATCAAGG 
TCTCAGACGC CCTTGAGTAC AGCAGCTGTC TCTTCCCAAG ACACTGCTAC CCCCGGTGCA 
CCGTTGCACA GGAACAAAGG GGCTGAGCCC TCACAGCAAG CCTTGGGTCC TGAACAGAAG 
CAGGCATCAA GATCAAGAAG CGAGCCACCG AGGGAAAGGA AGAAGGCTCC AGGGCTTTCA 
GAGCAGAATG GCAAGGGAGG CCAGAAGAGC GAGCGCAAAC GTGTCCCCAA GTCTGTGGTG 
CAACCCGGGG AAGGGATCGC GGATGAGAGG GAGAGGAAAG AGAGGCGGGA AACCCGCAGG 
TTGGAGAAAG GGCGCTCCCA GGACTACTCA GACCGGCCTG AGAAACGCGA CAATGGCAGG 
GTGGCGGAAG ACCAGAAGCA GAGGAAGGAG GAGGAGTACC AGACTAGGTA CCGCAGCGAC 
CCTAACCTGG CTCGCTACCC GGTGAAGGCG CCGCCAGAGG AGCAGCAGAT GCGCATGCAC 
GCCCGGGTGT CCCGAGCGAG GCACGAGCGG CGCCACAGCG ACGTGGCGCT CCCGCACACC 
GAGGCAGCTG CCGCCGCGCC GGCTGAGGCC ACGGCGGGCA AGCGCGCGCC GGCCACCGCC 
AGGGTCTCTC CCCCGGAGTC CCCGCGCGCA CGCGCGGCGG CCGCCCAGCC TCCCACCGAG 
CACGGGCCAC CGCCGCCGCG GCCAGCCCCG GGTCCCGCAG AGCCACCCGA GCCGCGCGTC 
CCCGAGCCGC TCCGTAAGCA GGGCCGCCTG GACCCGGGCT CGGCCGTGCT TCTGCGCAAG 
GCCAAGCGCG AGAAGGCGGA GAGCATGCTG CGGAACGACT CGCTGAGCTC CGATCAGTCC 
GAGTCCGTGC GGCCATCCCC GCCCAAGCCT CACCGGCCCA AGCGGGGAGG CAAGAGACGT 
CAGATGTCGG TGAGCAGCTC GGAGGAGGAG GGCGTGTCCA CACCGGAGTA CACGAGCTGC 
GAGGACGTGG AGCTGGAGAG CGAGAGCGTG AGCGAGAAAG GTGACTTGGA TTACTACTGG 
TTGGATCCCG CCACGTGGCA CAGCAGGGAA ACGTCGCCTA TCAGTTCGCA TCCTGTAACG 
TGGCAGCCGT CTAAAGAGGG AGATCGACTA ATCGGCCGTG TTATTCTTAA CAAAAGAACA 
ACCATGCCCA AAGAATCAGG TGCATTATTG GGTCTGAAGG TGGTTGGAGG AAAAATGACG 
GACTTAGGGC GCCTTGGTGC TTTCATCACC AAAGTAAAGA AGGGCAGCCT GGCAGACGTC 
GTCGGACACC TAAGAGCAGG GGACGAAGTC CTAGAGTGGA ATGGTAAACC CCTGCCGGGA 
GCAACAAACG AAGAAGTTTA CAACATTATC TTAGAATCAA AATCAGAACC TCAAGTTGAG 
ATTATTGTTT CAAGGCCTAT TGGTGACATC CCCAGGATCC CTGAGAGTTC CCATCCTCCC 
CTGGAGTCCA GTTCAAGTTC CTTTGAATCT CAGAAAATGG AAAGGCCTTC TATTTCTGTT 
ATTTCTCCAA CCAGCCCTGG AGCTCTGAAA GATGCCCCAC AAGTCTTACC AGGGCAACTC 
TCAGTGAAGC TATGGTATGA TAAAGTGGGG CACCAGCTGA TTGTAAATGT TCTACAAGCA 
ACAGATCTAC CCCCTAGAGT AGATGGCCGT CCCAGGAATC CCTATGTAAA AATGTATTTT 
CTTCCAGATA GAAGCGACAA AAGTAAAAGG AGAACCAAAA CAGTAAAGAA ACTTCTAGAG 
CCAAAATGGA ACCAGACATT TGTCTACTCA CACGTACATC GTAGAGATTT TCGAGAGCGA 
ATGTTAGAGA TTACCGTGTG GGACCAGCCG AGAGTACAGG ACGAAGAGAG TGAATTTCTT 
GGAGAGATCC TCATAGAGTT GGAAACAGCG CTTTTAGATG ATGAGCCCCA TTGGTATAAA 
CTCCAGACAC ATGACGAATC TTCACTACCT CTGCCTCAGC CATCACCGTT CATGCCCAGG 
CGGCATATTC ATGGAGAGAG CTCCAGCAAA AAGCTACAAA GATCTCAGCG AATCAGTGAT 
AGTGACATCT CAGATTATGA GGTTGATGAT GGTATTGGAG TAGTGCCTCC AGTGGGTTAT 
AGAGCTAGTG CTAGAGAGAG TAAAGCAACC ACGTTAACAG TGCCAGAGCA ACAAAGAACT 
ACACATCACC GCTCACGTTC CGTGTCTCCT CATCGCGGCG ATGATCAGGG AAGGCCTCGT 
TCACGTTTAC CAAATGTGCC ATTACAGAGG AGCTTAGATG AAATTCATCC AACACGAAGG 
TCACGTTCTC CAACCCGACA CCATGATGCC TCCCGAAGCC CGGCCGATCA CAGATCCAGA 
CATGTGGAAA GTCAATATTC GTCAGAGCCA GACAGTGAGC TTCTCATGCT GCCCAGAGCA 
AAACGAGGAC GAAGTGCAGA AAGCCTACAC ATGACCAGTG AACTGCAGCC CTCTCTTGAC 
AGGGCTAGGA GTGCTAGTAC CAACTGCTTG AGACCAGATA CTAGTTTGCA TTCACCAGAA 
CGAGAAAGGC ACTCCAGAAA GTCTGAAAGA TGTAGCATCC AAAAACAGTC TAGGAAAGGC 
ACAGCCTCTG ATGCAGACAG AACGCACCGA CAAGGAAGCC CAACCCAGTC TCCTCCAGCA 
GACACATCCT TCGGCAGTCG CCGTGGAAGA CAGCTCCCAC AGGTGCCAGT TCGAAGCGGC 
AGTATAGAAC AAGCAAGCTT AGTAGTGGAG GAGCGAACGA GACAGATGAA AGTGAAAGTT 
CACCGATTTA AGCAGACAAC AGGGTCTGGG TCTAGTCAAG AACTTGACCA CGAGCAATAC 
TCCAAGTACA ACATACATAA AGATCAGTAC AGAAGCTGTG ATAACGCGTC TGCCAAGTCT 
TCAGATAGTG ATGTCAGTGA TGTGTCCGCC ATTTCCAGAG CCAGCAGTAC CTCACGCCTC 
AGCAGCACAA GCTTTATGTC AGAGCAGTCT GAGCGCCCCA GGGGTAGGAT CAGTTCATTT 
ACCCCCAAAA TGCAAGGCAG ACGGATGGGG ACTTCAGGAA GAGCCATCAT CAAGAGCACC 
AGTGTAAGTG GAGAGATATA TACACTGGAA CGTAATGACG GTAGCCAGTC GGACACGGCC 
GTAGGTACCG TCGGAGCCGG TGGAAAGAAA CGAAGATCCA GCCTGAGCGC CAAAGTGGTA 
GCCATTGTGT CTCGAAGAAG CAGGAGCACG TCACAGCTCA GCCAGACAGA GTCGGGCCAC 
AAGAAGTTGA AAAGCACCAT CCAGAGGAGT ACGGAAACAG GAATGGCAGC TGAAATGCGG 
AAGATGGTGA GACAGCCGAG CCGGGAGTCC ACGGATGGCA GCATCAACAG TTATAGCTCG 
GAAGGAAACT TGATATTTCC TGGAGTTCGA GTAGGACCCG ACAGTCAGTT CAGTGATTTC 
CTTGATGGGT TGGGACCAGC GCAGCTCGTT GGCCGTCAGA CGCTCGCCAC CCCGGCCATG 
GGCGATATCC AAATCGGGAT GGAGGATAAG AAGGGTCAGT TGGAGGTTGA GGTTATCAGA 
GCCCGGAGCC TTACACAAAA ACCTGGTTCC AAATCTACAC CCGCTCCCTA TGTGAAAGTA 
TATCTTTTGG AAAATGGAGC CTGTATTGCC AAAAAGAAGA CAAGAATTGC ACGGAAAACT 
CTCGATCCTT TGTATCAGCA GTCCCTGGTT TTTGATGAAA GTCCACAGGG TAAAGTTCTT 
CAGGTGATTG TCTGGGGTGA CTATGGAAGA ATGGACCACA AATGCTTTAT GGGTGTGGCT 
CAAATCTTGT TGGAAGAACT TGATCTATCC AGCATGGTGA TTGGATGGTA TAAATTGTTC 
CCTCCGTCCT CACTGGTGGA TCCCACTCTC GCTCCCCTGA CCCGCCGGGC TTCCCAATCA 
TCTCTGGAAA GTTCGTCCGG GCCTCCCTGC ATCCGGTCAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rims1 ( XM_008766936.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008765158.1 Rattus norvegicus regulating synaptic membrane exocytosis 1 (Rims1), transcript variant X7, mRNA.

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CloneID	ORa43850
Clone ID Related Accession (Same CDS sequence)	XM_008766936.2
Accession Version	XM_008766936.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4845bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	regulating synaptic membrane exocytosis protein 1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005108.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008766936.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCTCGG CCGTGGGGCC CCGAGGTCCT CGCCCACCCA CGGTGCCTCC CCCTATGCAA 
GAACTGCCCG ACCTGAGCCA CCTGACCGAG GAGGAGAGGA ACATTATCAT GGCAGTGATG 
GACCGGCAGA AGGAAGAGGA GGAAAAAGAA GAGGCCATGC TCAAGTGTGT TGTCAGGGAC 
ATGGCGAAGC CTGCTGCCTG CAAAACACCA AGAAATGCTG AAAGCCAGCC CCATCAACCA 
CCACTGAACA TTTTCAGATG TGTCTGTGTT CCCAGAAAGC CAAGCAGCGA AGAGGGAGGC 
CCAGAAAGAG ACTGGAGATT GCATCAACAG TTTGAAAGCT ACAAGGAGCA AGTGAGAAAA 
ATCGGAGAGG AAGCGAGGCG TTACCAGGGC GAGCACAAGG ATGATGCCCC GACGTGTGGA 
ATCTGTCATA AGACAAAGTT TGCTGATGGA TGTGGCCATC TCTGCTCCTA TTGTCGCACC 
AAGTTCTGTG CACGCTGCGG AGGCCGTGTG TCTCTGCGAT CGAACAATGA GGACAAAGTG 
GTTATGTGGG TATGCAATTT ATGTCGAAAG CAACAAGAAA TCTTAACGAA ATCTGGAGCG 
TGGTTCTTTG GAAGTGGCCC TCAGCAGCCT AGTCAAGATG GGACTCTGAG TGACACGGCC 
ACAGGTGCTG GATCTGAGGT GCCAAGAGAA AAGAAAGCAA GGCTCCAAGA GCGATCAAGG 
TCTCAGACGC CCTTGAGTAC AGCAGCTGTC TCTTCCCAAG ACACTGCTAC CCCCGGTGCA 
CCGTTGGACA GGAACAAAGG GGCTGAGCCC TCACAGCAAG CCTTGGGTCC TGAACAGAAG 
CAGGCATCAA GATCAAGAAG CGAGCCACCG AGGGAAAGGA AGAAGGCTCC AGGGCTTTCA 
GAGCAGAATG GCAAGGGAGG CCAGAAGAGC GAGCGCAAAC GTGTCCCCAA GTCTGTGGTG 
CAACCCGGGG AAGGGATCGC GGATGAGAGG GAGAGGAAAG AGAGGCGGGA AACCCGCAGG 
TTGGAGAAAG GGCGCTCCCA GGACTACTCA GACCGGCCTG AGAAACGCGA CAATGGCAGG 
GTGGCGGAAG ACCAGAAGCA GAGGAAGGAG GAGGAGTACC AGACTAGGTA CCGCAGCGAC 
CCTAACCTGG CTCGCTACCC GGTGAAGGCG CCGCCAGAGG AGCAGCAGAT GCGCATGCAC 
GCCCGGGTGT CCCGAGCGAG GCACGAGCGG CGCCACAGCG ACGTGGCGCT CCCGCACACC 
GAGGCAGCTG CCGCCGCGCC GGCTGAGGCC ACGGCGGGCA AGCGCGCGCC GGCCACCGCC 
AGGGTCTCTC CCCCGGAGTC CCCGCGCGCA CGCGCGCCCG CCGCCCAGCC TCCCACCGAG 
CACGGGCCAC CGCCGCCGCG GCCAGTCCCG GGTCCCGCAG AGCCACCCGA GCCGCGCGTC 
CCCGAGCCGC TCCGTAAGCA GGGCCGCCTG GACCCGGCCT CGGCCGTGCT TCTGCGCAAG 
GCCAAGCGCG AGAAGGCGGA GAGCATGCTG CGGAACGACT CGCTGAGCTC CGATCAGTCC 
GAGTCCGTGC GGCCATCCCC GCCCAAGCCT CACCGGCCCA AGCGGGGAGG CAAGAGACGT 
CAGATGTCGG TGAGCAGCTC GGAGGAGGAG GGCGTGTCCA CACCGGAGTA CACGAGCTGC 
GAGGACGTGG AGCTGGAGAG CGAGAGCGTG AGCGAGAAAG GTGACTTGGA TTACTACTGG 
TTGGATCCCG CCACGTGGCA CAGCAGGGAA ACGTCGCCTA TCAGTTCGCA TCCTGTAACG 
TGGCAGCCGT CTAAAGAGGG AGATCGACTA ATCGGCCGTG TTATTCTTAA CAAAAGAACA 
ACCATGCCCA AAGAATCAGG TGCATTATTG GGTCTGAAGG TGGTTGGAGG AAAAATGACG 
GACTTAGGGC GCCTTGGTGC TTTCATCACC AAAGTAAAGA AGGGCAGCCT GGCAGACGTC 
GTCGGACACC TAAGAGCAGG GGACGAAGTC CTAGAGTGGA ATGGTAAACC CCTGCCGGGA 
GCAACAAACG AAGAAGTTTA CAACATTATC TTAGAATCAA AATCAGAACC TCAAGTTGAG 
ATTATTGTTT CAAGGCCTAT TGGTGACATC CCCAGGATCC CTGAGAGTTC CCATCCTCCC 
CTGGAGTCCA GTTCAAGTTC CTTTGAATCT CAGAAAATGG AAAGGCCTTC TATTTCTGTT 
ATTTCTCCAA CCAGCCCTGG AGCTCTGAAA GATGCCCCAC AAGTCTTACC AGGGCAACTC 
TCAGTGAAGC TATGGTATGA TAAAGTGGGG CACCAGCTGA TTGTAAATGT TCTACAAGCA 
ACAGATCTAC CCCCTAGAGT AGATGGCCGT CCCAGGAATC CCTATGTAAA AATGTATTTT 
CTTCCAGATA GAAGCGACAA AAGTAAAAGG AGAACCAAAA CAGTAAAGAA ACTTCTAGAG 
CCAAAATGGA ACCAGACATT TGTCTACTCA CACGTACATC GTAGAGATTT TCGAGAGCGA 
ATGTTAGAGA TTACCGTGTG GGACCAGCCG AGAGTACAGG ACGAAGAGAG TGAATTTCTT 
GGAGAGATCC TCATAGAGTT GGAAACAGCG CTTTTAGATG ATGAGCCCCA TTGGTATAAA 
CTCCAGACAC ATGACGAATC TTCACTACCT CTGCCTCAGC CATCACCGTT CATGCCCAGG 
CGGCATATTC ATGGAGAGAG CTCCAGCAAA AAGCTACAAA GATCTCAGCG AATCAGTGAT 
AGTGACATCT CAGATTATGA GGTTGATGAT GGTATTGGAG TAGTGCCTCC AGTGGGTTAT 
AGAGCTAGTG CTAGAGAGAG TAAAGCAACC ACGTTAACAG TGCCAGAGCA ACAAAGAACT 
ACACATCACC GCTCACGTTC CGTGTCTCCT CATCGCGGCG ATGATCAGGG AAGGCCTCGT 
TCACGTTTAC CAAATGTGCC ATTACAGAGG AGCTTAGATG AAATTCATCC AACACGAAGG 
TCACGTTCTC CAACCCGACA CCATGATGCC TCCCGAAGCC CGGCCGATCA CAGATCCAGA 
CATGTGGAAA GTCAATATTC GTCAGAGCCA GACAGTGAGC TTCTCATGCT GCCCAGAGCA 
AAACGAGGAC GAAGTGCAGA AAGCCTACAC ATGACCAGTG AACTGCAGCC CTCTCTTGAC 
AGGGCTAGGA GTGCTAGTAC CAACTGCTTG AGACCAGATA CTAGTTTGCA TTCACCAGAA 
CGAGAAAGGC ACTCCAGAAA GTCTGAAAGA TGTAGCATCC AAAAACAGTC TAGGAAAGGC 
ACAGCCTCTG ATGCAGACAG GGTTCTCCCA CCATGCCTTT CTAGAAGGGG ATACGCAACC 
CCAAGAGCAA CCGATCAACC GGTCGTTAGG GGAAAGCATC CCACTCGTTC ACGGTCGAGC 
GAGCACTCTA GTGTCAGAAC CCTGTGTTCT ATGCACCACC TTGCCCCCGG AGGGTCGGCG 
CCACCTTCTC CACTTCTGAC AAGAACGCAC CGACAAGGAA GCCCAACCCA GTCTCCTCCA 
GCAGACACAT CCTTCGGCAG TCGCCGTGGA AGACAGCTCC CACAGGTGCC AGTTCGAAGC 
GGCAGTATAG AACAAGCAAG CTTAGTAGTG GAGGAGCGAA CGAGACAGAT GAAAATGAAA 
GTTCACCGAT TTAAGCAGAC AACAGGGTCT GGGTCTAGTC AAGAACTTGA CCACGAGCAA 
TACTCCAAGT ACAACATACA TAAAGATCAG TACAGAAGCT GTGATAACGC GTCTGCCAAG 
TCTTCAGATA GTGATGTCAG TGATGTGTCC GCCATTTCCA GAGCCAGCAG TACCTCACGC 
CTCAGCAGCA CAAGCTTTAT GTCAGAGCAG TCTGAGCGCC CCAGGGGTAG GATCAGTTCA 
TTTACCCCCA AAATGCAAGG CAGACGGATG GGGACTTCAG GAAGAGCCAT CATCAAGAGC 
ACCAGTGTAA GTGGAGAGAT ATATACACTG GAACGTAATG ACGGTAGCCA GTCGGACACG 
GCCGTGGGTA CCGTCGGAGC CGGTGGAAAG AAACGAAGAT CCAGCCTGAG CGCCAAAGTG 
GTAGCCATTG TGTCTCGAAG AAGCAGGAGC ACGTCACAGC TCAGCCAGAC AGAGTCGGGC 
CACAAGAAGT TGAAAAGCAC CATCCAGAGG AGTACGGAAA CAGGAATGGC AGCTGAAATG 
CGGAAGATGG TGAGACAGCC GAGCCGGGAG TCCACGGATG GCAGCATCAA CAGTTATAGC 
TCGGAAGGAA ACTTGATATT TCCTGGAGTT CGAGTAGGAC CCGACAGTCA GTTCAGTGAT 
TTCCTTGATG GGTTGGGACC AGCGCAGCTC GTTGGCCGTC AGACGCTCGC CACCCCGGCC 
ATGGGCGATA TCCAAATCGG GATGGAGGAT AAGAAGGGTC AGTTGGAGGT TGAGGTTATC 
AGAGCCCGGA GCCTTACACA AAAACCTGGT TCCAAATCTA CACCCGCTCC CTATGTGAAA 
GTATATCTTT TGGAAAATGG AGCCTGTATT GCCAAAAAGA AGACAAGAAT TGCACGGAAA 
ACTCTCGATC CTTTGTATCA GCAGTCCCTG GTTTTTGATG AAAGTCCACA GGGTAAAGTT 
CTTCAGGTGA TTGTCTGGGG TGACTATGGA AGAATGGACC ACAAATGCTT TATGGGTGTG 
GCTCAAATCT TGTTGGAAGA ACTTGATCTA TCCAGCATGG TGATTGGATG GTATAAATTG 
TTCCCTCCGT CCTCACTGGT GGATCCCACT CTCGCTCCCC TGACCCGCCG GGCTTCCCAA 
TCATCTCTGG AAAGTTCGTC CGGGCCTCCC TGCATCCGGT CATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008765158.1
CDS	393..5237
Translation	MSSAVGPRGPRPPTVPPPMQELPDLSHLTEEERNIIMAVMDRQKEEEEKEEAMLKCVVRDMAKPAACKTPRNAESQPHQPPLNIFRCVCVPRKPSSEEGGPERDWRLHQQFESYKEQVRKIGEEARRYQGEHKDDAPTCGICHKTKFADGCGHLCSYCRTKFCARCGGRVSLRSNNEDKVVMWVCNLCRKQQEILTKSGAWFFGSGPQQPSQDGTLSDTATGAGSEVPREKKARLQERSRSQTPLSTAAVSSQDTATPGAPLDRNKGAEPSQQALGPEQKQASRSRSEPPRERKKAPGLSEQNGKGGQKSERKRVPKSVVQPGEGIADERERKERRETRRLEKGRSQDYSDRPEKRDNGRVAEDQKQRKEEEYQTRYRSDPNLARYPVKAPPEEQQMRMHARVSRARHERRHSDVALPHTEAAAAAPAEATAGKRAPATARVSPPESPRARAPAAQPPTEHGPPPPRPVPGPAEPPEPRVPEPLRKQGRLDPASAVLLRKAKREKAESMLRNDSLSSDQSESVRPSPPKPHRPKRGGKRRQMSVSSSEEEGVSTPEYTSCEDVELESESVSEKGDLDYYWLDPATWHSRETSPISSHPVTWQPSKEGDRLIGRVILNKRTTMPKESGALLGLKVVGGKMTDLGRLGAFITKVKKGSLADVVGHLRAGDEVLEWNGKPLPGATNEEVYNIILESKSEPQVEIIVSRPIGDIPRIPESSHPPLESSSSSFESQKMERPSISVISPTSPGALKDAPQVLPGQLSVKLWYDKVGHQLIVNVLQATDLPPRVDGRPRNPYVKMYFLPDRSDKSKRRTKTVKKLLEPKWNQTFVYSHVHRRDFRERMLEITVWDQPRVQDEESEFLGEILIELETALLDDEPHWYKLQTHDESSLPLPQPSPFMPRRHIHGESSSKKLQRSQRISDSDISDYEVDDGIGVVPPVGYRASARESKATTLTVPEQQRTTHHRSRSVSPHRGDDQGRPRSRLPNVPLQRSLDEIHPTRRSRSPTRHHDASRSPADHRSRHVESQYSSEPDSELLMLPRAKRGRSAESLHMTSELQPSLDRARSASTNCLRPDTSLHSPERERHSRKSERCSIQKQSRKGTASDADRVLPPCLSRRGYATPRATDQPVVRGKHPTRSRSSEHSSVRTLCSMHHLAPGGSAPPSPLLTRTHRQGSPTQSPPADTSFGSRRGRQLPQVPVRSGSIEQASLVVEERTRQMKMKVHRFKQTTGSGSSQELDHEQYSKYNIHKDQYRSCDNASAKSSDSDVSDVSAISRASSTSRLSSTSFMSEQSERPRGRISSFTPKMQGRRMGTSGRAIIKSTSVSGEIYTLERNDGSQSDTAVGTVGAGGKKRRSSLSAKVVAIVSRRSRSTSQLSQTESGHKKLKSTIQRSTETGMAAEMRKMVRQPSRESTDGSINSYSSEGNLIFPGVRVGPDSQFSDFLDGLGPAQLVGRQTLATPAMGDIQIGMEDKKGQLEVEVIRARSLTQKPGSKSTPAPYVKVYLLENGACIAKKKTRIARKTLDPLYQQSLVFDESPQGKVLQVIVWGDYGRMDHKCFMGVAQILLEELDLSSMVIGWYKLFPPSSLVDPTLAPLTRRASQSSLESSSGPPCIRS

Target ORF information:

RefSeq Version	XM_008766936.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus regulating synaptic membrane exocytosis 1 (Rims1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008766936.2

ORF Insert Sequence:

ATGTCCTCGG CCGTGGGGCC CCGAGGTCCT CGCCCACCCA CGGTGCCTCC CCCTATGCAA 
GAACTGCCCG ACCTGAGCCA CCTGACCGAG GAGGAGAGGA ACATTATCAT GGCAGTGATG 
GACCGGCAGA AGGAAGAGGA GGAAAAAGAA GAGGCCATGC TCAAGTGTGT TGTCAGGGAC 
ATGGCGAAGC CTGCTGCCTG CAAAACACCA AGAAATGCTG AAAGCCAGCC CCATCAACCA 
CCACTGAACA TTTTCAGATG TGTCTGTGTT CCCAGAAAGC CAAGCAGCGA AGAGGGAGGC 
CCAGAAAGAG ACTGGAGATT GCATCAACAG TTTGAAAGCT ACAAGGAGCA AGTGAGAAAA 
ATCGGAGAGG AAGCGAGGCG TTACCAGGGC GAGCACAAGG ATGATGCCCC GACGTGTGGA 
ATCTGTCATA AGACAAAGTT TGCTGATGGA TGTGGCCATC TCTGCTCCTA TTGTCGCACC 
AAGTTCTGTG CACGCTGCGG AGGCCGTGTG TCTCTGCGAT CGAACAATGA GGACAAAGTG 
GTTATGTGGG TATGCAATTT ATGTCGAAAG CAACAAGAAA TCTTAACGAA ATCTGGAGCG 
TGGTTCTTTG GAAGTGGCCC TCAGCAGCCT AGTCAAGATG GGACTCTGAG TGACACGGCC 
ACAGGTGCTG GATCTGAGGT GCCAAGAGAA AAGAAAGCAA GGCTCCAAGA GCGATCAAGG 
TCTCAGACGC CCTTGAGTAC AGCAGCTGTC TCTTCCCAAG ACACTGCTAC CCCCGGTGCA 
CCGTTGGACA GGAACAAAGG GGCTGAGCCC TCACAGCAAG CCTTGGGTCC TGAACAGAAG 
CAGGCATCAA GATCAAGAAG CGAGCCACCG AGGGAAAGGA AGAAGGCTCC AGGGCTTTCA 
GAGCAGAATG GCAAGGGAGG CCAGAAGAGC GAGCGCAAAC GTGTCCCCAA GTCTGTGGTG 
CAACCCGGGG AAGGGATCGC GGATGAGAGG GAGAGGAAAG AGAGGCGGGA AACCCGCAGG 
TTGGAGAAAG GGCGCTCCCA GGACTACTCA GACCGGCCTG AGAAACGCGA CAATGGCAGG 
GTGGCGGAAG ACCAGAAGCA GAGGAAGGAG GAGGAGTACC AGACTAGGTA CCGCAGCGAC 
CCTAACCTGG CTCGCTACCC GGTGAAGGCG CCGCCAGAGG AGCAGCAGAT GCGCATGCAC 
GCCCGGGTGT CCCGAGCGAG GCACGAGCGG CGCCACAGCG ACGTGGCGCT CCCGCACACC 
GAGGCAGCTG CCGCCGCGCC GGCTGAGGCC ACGGCGGGCA AGCGCGCGCC GGCCACCGCC 
AGGGTCTCTC CCCCGGAGTC CCCGCGCGCA CGCGCGCCCG CCGCCCAGCC TCCCACCGAG 
CACGGGCCAC CGCCGCCGCG GCCAGTCCCG GGTCCCGCAG AGCCACCCGA GCCGCGCGTC 
CCCGAGCCGC TCCGTAAGCA GGGCCGCCTG GACCCGGCCT CGGCCGTGCT TCTGCGCAAG 
GCCAAGCGCG AGAAGGCGGA GAGCATGCTG CGGAACGACT CGCTGAGCTC CGATCAGTCC 
GAGTCCGTGC GGCCATCCCC GCCCAAGCCT CACCGGCCCA AGCGGGGAGG CAAGAGACGT 
CAGATGTCGG TGAGCAGCTC GGAGGAGGAG GGCGTGTCCA CACCGGAGTA CACGAGCTGC 
GAGGACGTGG AGCTGGAGAG CGAGAGCGTG AGCGAGAAAG GTGACTTGGA TTACTACTGG 
TTGGATCCCG CCACGTGGCA CAGCAGGGAA ACGTCGCCTA TCAGTTCGCA TCCTGTAACG 
TGGCAGCCGT CTAAAGAGGG AGATCGACTA ATCGGCCGTG TTATTCTTAA CAAAAGAACA 
ACCATGCCCA AAGAATCAGG TGCATTATTG GGTCTGAAGG TGGTTGGAGG AAAAATGACG 
GACTTAGGGC GCCTTGGTGC TTTCATCACC AAAGTAAAGA AGGGCAGCCT GGCAGACGTC 
GTCGGACACC TAAGAGCAGG GGACGAAGTC CTAGAGTGGA ATGGTAAACC CCTGCCGGGA 
GCAACAAACG AAGAAGTTTA CAACATTATC TTAGAATCAA AATCAGAACC TCAAGTTGAG 
ATTATTGTTT CAAGGCCTAT TGGTGACATC CCCAGGATCC CTGAGAGTTC CCATCCTCCC 
CTGGAGTCCA GTTCAAGTTC CTTTGAATCT CAGAAAATGG AAAGGCCTTC TATTTCTGTT 
ATTTCTCCAA CCAGCCCTGG AGCTCTGAAA GATGCCCCAC AAGTCTTACC AGGGCAACTC 
TCAGTGAAGC TATGGTATGA TAAAGTGGGG CACCAGCTGA TTGTAAATGT TCTACAAGCA 
ACAGATCTAC CCCCTAGAGT AGATGGCCGT CCCAGGAATC CCTATGTAAA AATGTATTTT 
CTTCCAGATA GAAGCGACAA AAGTAAAAGG AGAACCAAAA CAGTAAAGAA ACTTCTAGAG 
CCAAAATGGA ACCAGACATT TGTCTACTCA CACGTACATC GTAGAGATTT TCGAGAGCGA 
ATGTTAGAGA TTACCGTGTG GGACCAGCCG AGAGTACAGG ACGAAGAGAG TGAATTTCTT 
GGAGAGATCC TCATAGAGTT GGAAACAGCG CTTTTAGATG ATGAGCCCCA TTGGTATAAA 
CTCCAGACAC ATGACGAATC TTCACTACCT CTGCCTCAGC CATCACCGTT CATGCCCAGG 
CGGCATATTC ATGGAGAGAG CTCCAGCAAA AAGCTACAAA GATCTCAGCG AATCAGTGAT 
AGTGACATCT CAGATTATGA GGTTGATGAT GGTATTGGAG TAGTGCCTCC AGTGGGTTAT 
AGAGCTAGTG CTAGAGAGAG TAAAGCAACC ACGTTAACAG TGCCAGAGCA ACAAAGAACT 
ACACATCACC GCTCACGTTC CGTGTCTCCT CATCGCGGCG ATGATCAGGG AAGGCCTCGT 
TCACGTTTAC CAAATGTGCC ATTACAGAGG AGCTTAGATG AAATTCATCC AACACGAAGG 
TCACGTTCTC CAACCCGACA CCATGATGCC TCCCGAAGCC CGGCCGATCA CAGATCCAGA 
CATGTGGAAA GTCAATATTC GTCAGAGCCA GACAGTGAGC TTCTCATGCT GCCCAGAGCA 
AAACGAGGAC GAAGTGCAGA AAGCCTACAC ATGACCAGTG AACTGCAGCC CTCTCTTGAC 
AGGGCTAGGA GTGCTAGTAC CAACTGCTTG AGACCAGATA CTAGTTTGCA TTCACCAGAA 
CGAGAAAGGC ACTCCAGAAA GTCTGAAAGA TGTAGCATCC AAAAACAGTC TAGGAAAGGC 
ACAGCCTCTG ATGCAGACAG GGTTCTCCCA CCATGCCTTT CTAGAAGGGG ATACGCAACC 
CCAAGAGCAA CCGATCAACC GGTCGTTAGG GGAAAGCATC CCACTCGTTC ACGGTCGAGC 
GAGCACTCTA GTGTCAGAAC CCTGTGTTCT ATGCACCACC TTGCCCCCGG AGGGTCGGCG 
CCACCTTCTC CACTTCTGAC AAGAACGCAC CGACAAGGAA GCCCAACCCA GTCTCCTCCA 
GCAGACACAT CCTTCGGCAG TCGCCGTGGA AGACAGCTCC CACAGGTGCC AGTTCGAAGC 
GGCAGTATAG AACAAGCAAG CTTAGTAGTG GAGGAGCGAA CGAGACAGAT GAAAATGAAA 
GTTCACCGAT TTAAGCAGAC AACAGGGTCT GGGTCTAGTC AAGAACTTGA CCACGAGCAA 
TACTCCAAGT ACAACATACA TAAAGATCAG TACAGAAGCT GTGATAACGC GTCTGCCAAG 
TCTTCAGATA GTGATGTCAG TGATGTGTCC GCCATTTCCA GAGCCAGCAG TACCTCACGC 
CTCAGCAGCA CAAGCTTTAT GTCAGAGCAG TCTGAGCGCC CCAGGGGTAG GATCAGTTCA 
TTTACCCCCA AAATGCAAGG CAGACGGATG GGGACTTCAG GAAGAGCCAT CATCAAGAGC 
ACCAGTGTAA GTGGAGAGAT ATATACACTG GAACGTAATG ACGGTAGCCA GTCGGACACG 
GCCGTGGGTA CCGTCGGAGC CGGTGGAAAG AAACGAAGAT CCAGCCTGAG CGCCAAAGTG 
GTAGCCATTG TGTCTCGAAG AAGCAGGAGC ACGTCACAGC TCAGCCAGAC AGAGTCGGGC 
CACAAGAAGT TGAAAAGCAC CATCCAGAGG AGTACGGAAA CAGGAATGGC AGCTGAAATG 
CGGAAGATGG TGAGACAGCC GAGCCGGGAG TCCACGGATG GCAGCATCAA CAGTTATAGC 
TCGGAAGGAA ACTTGATATT TCCTGGAGTT CGAGTAGGAC CCGACAGTCA GTTCAGTGAT 
TTCCTTGATG GGTTGGGACC AGCGCAGCTC GTTGGCCGTC AGACGCTCGC CACCCCGGCC 
ATGGGCGATA TCCAAATCGG GATGGAGGAT AAGAAGGGTC AGTTGGAGGT TGAGGTTATC 
AGAGCCCGGA GCCTTACACA AAAACCTGGT TCCAAATCTA CACCCGCTCC CTATGTGAAA 
GTATATCTTT TGGAAAATGG AGCCTGTATT GCCAAAAAGA AGACAAGAAT TGCACGGAAA 
ACTCTCGATC CTTTGTATCA GCAGTCCCTG GTTTTTGATG AAAGTCCACA GGGTAAAGTT 
CTTCAGGTGA TTGTCTGGGG TGACTATGGA AGAATGGACC ACAAATGCTT TATGGGTGTG 
GCTCAAATCT TGTTGGAAGA ACTTGATCTA TCCAGCATGG TGATTGGATG GTATAAATTG 
TTCCCTCCGT CCTCACTGGT GGATCCCACT CTCGCTCCCC TGACCCGCCG GGCTTCCCAA 
TCATCTCTGG AAAGTTCGTC CGGGCCTCCC TGCATCCGGT CATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rims1 ( XM_017596655.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017452144.1 Rattus norvegicus regulating synaptic membrane exocytosis 1 (Rims1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa62872
Clone ID Related Accession (Same CDS sequence)	XM_017596655.1
Accession Version	XM_017596655.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4950bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	regulating synaptic membrane exocytosis protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005108.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCTCGG CCGTGGGGCC CCGAGGTCCT CGCCCACCCA CGGTGCCTCC CCCTATGCAA 
GAACTGCCCG ACCTGAGCCA CCTGACCGAG GAGGAGAGGA ACATTATCAT GGCAGTGATG 
GACCGGCAGA AGGAAGAGGA GGAAAAAGAA GAGGCCATGC TCAAGTGTGT TGTCAGGGAC 
ATGGCGAAGC CTGCTGCCTG CAAAACACCA AGAAATGCTG AAAGCCAGCC CCATCAACCA 
CCACTGAACA TTTTCAGATG TGTCTGTGTT CCCAGAAAGC CAAGCAGCGA AGAGGGAGGC 
CCAGAAAGAG ACTGGAGATT GCATCAACAG TTTGAAAGCT ACAAGGAGCA AGTGAGAAAA 
ATCGGAGAGG AAGCGAGGCG TTACCAGGGC GAGCACAAGG ATGATGCCCC GACGTGTGGA 
ATCTGTCATA AGACAAAGTT TGCTGATGGA TGTGGCCATC TCTGCTCCTA TTGTCGCACC 
AAGTTCTGTG CACGCTGCGG AGGCCGTGTG TCTCTGCGAT CGAACAATGA GGACAAAGTG 
GTTATGTGGG TATGCAATTT ATGTCGAAAG CAACAAGAAA TCTTAACGAA ATCTGGAGCG 
TGGTTCTTTG GAAGTGGCCC TCAGCAGCCT AGTCAAGATG GGACTCTGAG TGACACGGCC 
ACAGGTGCTG GATCTGAGGT GCCAAGAGAA AAGAAAGCAA GGCTCCAAGA GCGATCAAGG 
TCTCAGACGC CCTTGAGTAC AGCAGCTGTC TCTTCCCAAG ACACTGCTAC CCCCGGTGCA 
CCGTTGGACA GGAACAAAGG GGCTGAGCCC TCACAGCAAG CCTTGGGTCC TGAACAGAAG 
CAGGCATCAA GATCAAGAAG CGAGCCACCG AGGGAAAGGA AGAAGGCTCC AGGGCTTTCA 
GAGCAGAATG GCAAGGGAGG CCAGAAGAGC GAGCGCAAAC GTGTCCCCAA GTCTGTGGTG 
CAACCCGGGG AAGGGATCGC GGATGAGAGG GAGAGGAAAG AGAGGCGGGA AACCCGCAGG 
TTGGAGAAAG GGCGCTCCCA GGACTACTCA GACCGGCCTG AGAAACGCGA CAATGGCAGG 
GTGGCGGAAG ACCAGAAGCA GAGGAAGGAG GAGGAGTACC AGACTAGGTA CCGCAGCGAC 
CCTAACCTGG CTCGCTACCC GGTGAAGGCG CCGCCAGAGG AGCAGCAGAT GCGCATGCAC 
GCCCGGGTGT CCCGAGCGAG GCACGAGCGG CGCCACAGCG ACGTGGCGCT CCCGCACACC 
GAGGCAGCTG CCGCCGCGCC GGCTGAGGCC ACGGCGGGCA AGCGCGCGCC GGCCACCGCC 
AGGGTCTCTC CCCCGGAGTC CCCGCGCGCA CGCGCGCCCG CCGCCCAGCC TCCCACCGAG 
CACGGGCCAC CGCCGCCGCG GCCAGTCCCG GGTCCCGCAG AGCCACCCGA GCCGCGCGTC 
CCCGAGCCGC TCCGTAAGCA GGGCCGCCTG GACCCGGCCT CGGCCGTGCT TCTGCGCAAG 
GCCAAGCGCG AGAAGGCGGA GAGCATGCTG CGGAACGACT CGCTGAGCTC CGATCAGTCC 
GAGTCCGTGC GGCCATCCCC GCCCAAGCCT CACCGGCCCA AGCGGGGAGG CAAGAGACGT 
CAGATGTCGG TGAGCAGCTC GGAGGAGGAG GGCGTGTCCA CACCGGAGTA CACGAGCTGC 
GAGGACGTGG AGCTGGAGAG CGAGAGCGTG AGCGAGAAAG GTGACTTGGA TTACTACTGG 
TTGGATCCCG CCACGTGGCA CAGCAGGGAA ACGTCGCCTA TCAGTTCGCA TCCTGTAACG 
TGGCAGCCGT CTAAAGAGGG AGATCGACTA ATCGGCCGTG TTATTCTTAA CAAAAGAACA 
ACCATGCCCA AAGAATCAGG TGCATTATTG GGTCTGAAGG TGGTTGGAGG AAAAATGACG 
GACTTAGGGC GCCTTGGTGC TTTCATCACC AAAGTAAAGA AGGGCAGCCT GGCAGACGTC 
GTCGGACACC TAAGAGCAGG GGACGAAGTC CTAGAGTGGA ATGGTAAACC CCTGCCGGGA 
GCAACAAACG AAGAAGTTTA CAACATTATC TTAGAATCAA AATCAGAACC TCAAGTTGAG 
ATTATTGTTT CAAGGCCTAT TGGTGACATC CCCAGGATCC CTGAGAGTTC CCATCCTCCC 
CTGGAGTCCA GTTCAAGTTC CTTTGAATCT CAGAAAATGG AAAGGCCTTC TATTTCTGTT 
ATTTCTCCAA CCAGCCCTGG AGCTCTGAAA GATGCCCCAC AAGTCTTACC AGGGCAACTC 
TCAGTGAAGC TATGGTATGA TAAAGTGGGG CACCAGCTGA TTGTAAATGT TCTACAAGCA 
ACAGATCTAC CCCCTAGAGT AGATGGCCGT CCCAGGAATC CCTATGTAAA AATGTATTTT 
CTTCCAGATA GAAGCGACAA AAGTAAAAGG AGAACCAAAA CAGTAAAGAA ACTTCTAGAG 
CCAAAATGGA ACCAGACATT TGTCTACTCA CACGTACATC GTAGAGATTT TCGAGAGCGA 
ATGTTAGAGA TTACCGTGTG GGACCAGCCG AGAGTACAGG ACGAAGAGAG TGAATTTCTT 
GGAGAGATCC TCATAGAGTT GGAAACAGCG CTTTTAGATG ATGAGCCCCA TTGGTATAAA 
CTCCAGACAC ATGACGAATC TTCACTACCT CTGCCTCAGC CATCACCGTT CATGCCCAGG 
CGGCATATTC ATGGAGAGAG CTCCAGCAAA AAGCTACAAA GATCTCAGCG AATCAGTGAT 
AGTGACATCT CAGATTATGA GGTTGATGAT GGTATTGGAG TAGTGCCTCC AGTGGGTTAT 
AGAGCTAGTG CTAGAGAGAG TAAAGCAACC ACGTTAACAG TGCCAGAGCA ACAAAGAACT 
ACACATCACC GCTCACGTTC CGTGTCTCCT CATCGCGGCG ATGATCAGGG AAGGCCTCGT 
TCACGTTTAC CAAATGTGCC ATTACAGAGG AGCTTAGATG AAATTCATCC AACACGAAGG 
TCACGTTCTC CAACCCGACA CCATGATGCC TCCCGAAGCC CGGCCGATCA CAGATCCAGA 
CATGTGGAAA GTCAATATTC GTCAGAGCCA GACAGTGAGC TTCTCATGCT GCCCAGAGCA 
AAACGAGGAC GAAGTGCAGA AAGCCTACAC ATGACCAGTG AACTGCAGCC CTCTCTTGAC 
AGGGCTAGGA GTGCTAGTAC CAACTGCTTG AGACCAGATA CTAGTTTGCA TTCACCAGAA 
CGAGAAAGGG GTAGATGGTC CCCCTCCCTA GATAGGAGGC GACCTGCTAG CCCCAGGATT 
CAAATCCAGC ATGCATCTCC GGAGAATGAC AGGCACTCCA GAAAGTCTGA AAGATGTAGC 
ATCCAAAAAC AGTCTAGGAA AGGCACAGCC TCTGATGCAG ACAGGGTTCT CCCACCATGC 
CTTTCTAGAA GGGGATACGC AACCCCAAGA GCAACCGATC AACCGGTCGT TAGGGGAAAG 
CATCCCACTC GTTCACGGTC GAGCGAGCAC TCTAGTGTCA GAACCCTGTG TTCTATGCAC 
CACCTTGCCC CCGGAGGGTC GGCGCCACCT TCTCCACTTC TGACAAGAAC GCACCGACAA 
GGAAGCCCAA CCCAGTCTCC TCCAGCAGAC ACATCCTTCG GCAGTCGCCG TGGAAGACAG 
CTCCCACAGG TGCCAGTTCG AAGCGGCAGT ATAGAACAAG CAAGCTTAGT AGTGGAGGAG 
CGAACGAGAC AGATGAAAAT GAAAGTTCAC CGATTTAAGC AGACAACAGG GTCTGGGTCT 
AGTCAAGAAC TTGACCACGA GCAATACTCC AAGAAGTCAC GTTACATTAT GGAGTACAAC 
ATACATAAAG ATCAGTACAG AAGCTGTGAT AACGCGTCTG CCAAGTCTTC AGATAGTGAT 
GTCAGTGATG TGTCCGCCAT TTCCAGAGCC AGCAGTACCT CACGCCTCAG CAGCACAAGC 
TTTATGTCAG AGCAGTCTGA GCGCCCCAGG GGTAGGATCA GTTCATTTAC CCCCAAAATG 
CAAGGCAGAC GGATGGGGAC TTCAGGAAGA GCCATCATCA AGAGCACCAG TGTAAGTGGA 
GAGATATATA CACTGGAACG TAATGACGGT AGCCAGTCGG ACACGGCCGT GGGTACCGTC 
GGAGCCGGTG GAAAGAAACG AAGATCCAGC CTGAGCGCCA AAGTGGTAGC CATTGTGTCT 
CGAAGAAGCA GGAGCACGTC ACAGCTCAGC CAGACAGAGT CGGGCCACAA GAAGTTGAAA 
AGCACCATCC AGAGGAGTAC GGAAACAGGA ATGGCAGCTG AAATGCGGAA GATGGTGAGA 
CAGCCGAGCC GGGAGTCCAC GGATGGCAGC ATCAACAGTT ATAGCTCGGA AGGAAACTTG 
ATATTTCCTG GAGTTCGAGT AGGACCCGAC AGTCAGTTCA GTGATTTCCT TGATGGGTTG 
GGACCAGCGC AGCTCGTTGG CCGTCAGACG CTCGCCACCC CGGCCATGGG CGATATCCAA 
ATCGGGATGG AGGATAAGAA GGGTCAGTTG GAGGTTGAGG TTATCAGAGC CCGGAGCCTT 
ACACAAAAAC CTGGTTCCAA ATCTACACCC GCTCCCTATG TGAAAGTATA TCTTTTGGAA 
AATGGAGCCT GTATTGCCAA AAAGAAGACA AGAATTGCAC GGAAAACTCT CGATCCTTTG 
TATCAGCAGT CCCTGGTTTT TGATGAAAGT CCACAGGGTA AAGTTCTTCA GGTGATTGTC 
TGGGGTGACT ATGGAAGAAT GGACCACAAA TGCTTTATGG GTGTGGCTCA AATCTTGTTG 
GAAGAACTTG ATCTATCCAG CATGGTGATT GGATGGTATA AATTGTTCCC TCCGTCCTCA 
CTGGTGGATC CCACTCTCGC TCCCCTGACC CGCCGGGCTT CCCAATCATC TCTGGAAAGT 
TCGTCCGGGC CTCCCTGCAT CCGGTCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017452144.1
CDS	316..5265
Translation	MSSAVGPRGPRPPTVPPPMQELPDLSHLTEEERNIIMAVMDRQKEEEEKEEAMLKCVVRDMAKPAACKTPRNAESQPHQPPLNIFRCVCVPRKPSSEEGGPERDWRLHQQFESYKEQVRKIGEEARRYQGEHKDDAPTCGICHKTKFADGCGHLCSYCRTKFCARCGGRVSLRSNNEDKVVMWVCNLCRKQQEILTKSGAWFFGSGPQQPSQDGTLSDTATGAGSEVPREKKARLQERSRSQTPLSTAAVSSQDTATPGAPLDRNKGAEPSQQALGPEQKQASRSRSEPPRERKKAPGLSEQNGKGGQKSERKRVPKSVVQPGEGIADERERKERRETRRLEKGRSQDYSDRPEKRDNGRVAEDQKQRKEEEYQTRYRSDPNLARYPVKAPPEEQQMRMHARVSRARHERRHSDVALPHTEAAAAAPAEATAGKRAPATARVSPPESPRARAPAAQPPTEHGPPPPRPVPGPAEPPEPRVPEPLRKQGRLDPASAVLLRKAKREKAESMLRNDSLSSDQSESVRPSPPKPHRPKRGGKRRQMSVSSSEEEGVSTPEYTSCEDVELESESVSEKGDLDYYWLDPATWHSRETSPISSHPVTWQPSKEGDRLIGRVILNKRTTMPKESGALLGLKVVGGKMTDLGRLGAFITKVKKGSLADVVGHLRAGDEVLEWNGKPLPGATNEEVYNIILESKSEPQVEIIVSRPIGDIPRIPESSHPPLESSSSSFESQKMERPSISVISPTSPGALKDAPQVLPGQLSVKLWYDKVGHQLIVNVLQATDLPPRVDGRPRNPYVKMYFLPDRSDKSKRRTKTVKKLLEPKWNQTFVYSHVHRRDFRERMLEITVWDQPRVQDEESEFLGEILIELETALLDDEPHWYKLQTHDESSLPLPQPSPFMPRRHIHGESSSKKLQRSQRISDSDISDYEVDDGIGVVPPVGYRASARESKATTLTVPEQQRTTHHRSRSVSPHRGDDQGRPRSRLPNVPLQRSLDEIHPTRRSRSPTRHHDASRSPADHRSRHVESQYSSEPDSELLMLPRAKRGRSAESLHMTSELQPSLDRARSASTNCLRPDTSLHSPERERGRWSPSLDRRRPASPRIQIQHASPENDRHSRKSERCSIQKQSRKGTASDADRVLPPCLSRRGYATPRATDQPVVRGKHPTRSRSSEHSSVRTLCSMHHLAPGGSAPPSPLLTRTHRQGSPTQSPPADTSFGSRRGRQLPQVPVRSGSIEQASLVVEERTRQMKMKVHRFKQTTGSGSSQELDHEQYSKKSRYIMEYNIHKDQYRSCDNASAKSSDSDVSDVSAISRASSTSRLSSTSFMSEQSERPRGRISSFTPKMQGRRMGTSGRAIIKSTSVSGEIYTLERNDGSQSDTAVGTVGAGGKKRRSSLSAKVVAIVSRRSRSTSQLSQTESGHKKLKSTIQRSTETGMAAEMRKMVRQPSRESTDGSINSYSSEGNLIFPGVRVGPDSQFSDFLDGLGPAQLVGRQTLATPAMGDIQIGMEDKKGQLEVEVIRARSLTQKPGSKSTPAPYVKVYLLENGACIAKKKTRIARKTLDPLYQQSLVFDESPQGKVLQVIVWGDYGRMDHKCFMGVAQILLEELDLSSMVIGWYKLFPPSSLVDPTLAPLTRRASQSSLESSSGPPCIRS

Target ORF information:

RefSeq Version	XM_017596655.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus regulating synaptic membrane exocytosis 1 (Rims1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017596655.1

ORF Insert Sequence:

ATGTCCTCGG CCGTGGGGCC CCGAGGTCCT CGCCCACCCA CGGTGCCTCC CCCTATGCAA 
GAACTGCCCG ACCTGAGCCA CCTGACCGAG GAGGAGAGGA ACATTATCAT GGCAGTGATG 
GACCGGCAGA AGGAAGAGGA GGAAAAAGAA GAGGCCATGC TCAAGTGTGT TGTCAGGGAC 
ATGGCGAAGC CTGCTGCCTG CAAAACACCA AGAAATGCTG AAAGCCAGCC CCATCAACCA 
CCACTGAACA TTTTCAGATG TGTCTGTGTT CCCAGAAAGC CAAGCAGCGA AGAGGGAGGC 
CCAGAAAGAG ACTGGAGATT GCATCAACAG TTTGAAAGCT ACAAGGAGCA AGTGAGAAAA 
ATCGGAGAGG AAGCGAGGCG TTACCAGGGC GAGCACAAGG ATGATGCCCC GACGTGTGGA 
ATCTGTCATA AGACAAAGTT TGCTGATGGA TGTGGCCATC TCTGCTCCTA TTGTCGCACC 
AAGTTCTGTG CACGCTGCGG AGGCCGTGTG TCTCTGCGAT CGAACAATGA GGACAAAGTG 
GTTATGTGGG TATGCAATTT ATGTCGAAAG CAACAAGAAA TCTTAACGAA ATCTGGAGCG 
TGGTTCTTTG GAAGTGGCCC TCAGCAGCCT AGTCAAGATG GGACTCTGAG TGACACGGCC 
ACAGGTGCTG GATCTGAGGT GCCAAGAGAA AAGAAAGCAA GGCTCCAAGA GCGATCAAGG 
TCTCAGACGC CCTTGAGTAC AGCAGCTGTC TCTTCCCAAG ACACTGCTAC CCCCGGTGCA 
CCGTTGGACA GGAACAAAGG GGCTGAGCCC TCACAGCAAG CCTTGGGTCC TGAACAGAAG 
CAGGCATCAA GATCAAGAAG CGAGCCACCG AGGGAAAGGA AGAAGGCTCC AGGGCTTTCA 
GAGCAGAATG GCAAGGGAGG CCAGAAGAGC GAGCGCAAAC GTGTCCCCAA GTCTGTGGTG 
CAACCCGGGG AAGGGATCGC GGATGAGAGG GAGAGGAAAG AGAGGCGGGA AACCCGCAGG 
TTGGAGAAAG GGCGCTCCCA GGACTACTCA GACCGGCCTG AGAAACGCGA CAATGGCAGG 
GTGGCGGAAG ACCAGAAGCA GAGGAAGGAG GAGGAGTACC AGACTAGGTA CCGCAGCGAC 
CCTAACCTGG CTCGCTACCC GGTGAAGGCG CCGCCAGAGG AGCAGCAGAT GCGCATGCAC 
GCCCGGGTGT CCCGAGCGAG GCACGAGCGG CGCCACAGCG ACGTGGCGCT CCCGCACACC 
GAGGCAGCTG CCGCCGCGCC GGCTGAGGCC ACGGCGGGCA AGCGCGCGCC GGCCACCGCC 
AGGGTCTCTC CCCCGGAGTC CCCGCGCGCA CGCGCGCCCG CCGCCCAGCC TCCCACCGAG 
CACGGGCCAC CGCCGCCGCG GCCAGTCCCG GGTCCCGCAG AGCCACCCGA GCCGCGCGTC 
CCCGAGCCGC TCCGTAAGCA GGGCCGCCTG GACCCGGCCT CGGCCGTGCT TCTGCGCAAG 
GCCAAGCGCG AGAAGGCGGA GAGCATGCTG CGGAACGACT CGCTGAGCTC CGATCAGTCC 
GAGTCCGTGC GGCCATCCCC GCCCAAGCCT CACCGGCCCA AGCGGGGAGG CAAGAGACGT 
CAGATGTCGG TGAGCAGCTC GGAGGAGGAG GGCGTGTCCA CACCGGAGTA CACGAGCTGC 
GAGGACGTGG AGCTGGAGAG CGAGAGCGTG AGCGAGAAAG GTGACTTGGA TTACTACTGG 
TTGGATCCCG CCACGTGGCA CAGCAGGGAA ACGTCGCCTA TCAGTTCGCA TCCTGTAACG 
TGGCAGCCGT CTAAAGAGGG AGATCGACTA ATCGGCCGTG TTATTCTTAA CAAAAGAACA 
ACCATGCCCA AAGAATCAGG TGCATTATTG GGTCTGAAGG TGGTTGGAGG AAAAATGACG 
GACTTAGGGC GCCTTGGTGC TTTCATCACC AAAGTAAAGA AGGGCAGCCT GGCAGACGTC 
GTCGGACACC TAAGAGCAGG GGACGAAGTC CTAGAGTGGA ATGGTAAACC CCTGCCGGGA 
GCAACAAACG AAGAAGTTTA CAACATTATC TTAGAATCAA AATCAGAACC TCAAGTTGAG 
ATTATTGTTT CAAGGCCTAT TGGTGACATC CCCAGGATCC CTGAGAGTTC CCATCCTCCC 
CTGGAGTCCA GTTCAAGTTC CTTTGAATCT CAGAAAATGG AAAGGCCTTC TATTTCTGTT 
ATTTCTCCAA CCAGCCCTGG AGCTCTGAAA GATGCCCCAC AAGTCTTACC AGGGCAACTC 
TCAGTGAAGC TATGGTATGA TAAAGTGGGG CACCAGCTGA TTGTAAATGT TCTACAAGCA 
ACAGATCTAC CCCCTAGAGT AGATGGCCGT CCCAGGAATC CCTATGTAAA AATGTATTTT 
CTTCCAGATA GAAGCGACAA AAGTAAAAGG AGAACCAAAA CAGTAAAGAA ACTTCTAGAG 
CCAAAATGGA ACCAGACATT TGTCTACTCA CACGTACATC GTAGAGATTT TCGAGAGCGA 
ATGTTAGAGA TTACCGTGTG GGACCAGCCG AGAGTACAGG ACGAAGAGAG TGAATTTCTT 
GGAGAGATCC TCATAGAGTT GGAAACAGCG CTTTTAGATG ATGAGCCCCA TTGGTATAAA 
CTCCAGACAC ATGACGAATC TTCACTACCT CTGCCTCAGC CATCACCGTT CATGCCCAGG 
CGGCATATTC ATGGAGAGAG CTCCAGCAAA AAGCTACAAA GATCTCAGCG AATCAGTGAT 
AGTGACATCT CAGATTATGA GGTTGATGAT GGTATTGGAG TAGTGCCTCC AGTGGGTTAT 
AGAGCTAGTG CTAGAGAGAG TAAAGCAACC ACGTTAACAG TGCCAGAGCA ACAAAGAACT 
ACACATCACC GCTCACGTTC CGTGTCTCCT CATCGCGGCG ATGATCAGGG AAGGCCTCGT 
TCACGTTTAC CAAATGTGCC ATTACAGAGG AGCTTAGATG AAATTCATCC AACACGAAGG 
TCACGTTCTC CAACCCGACA CCATGATGCC TCCCGAAGCC CGGCCGATCA CAGATCCAGA 
CATGTGGAAA GTCAATATTC GTCAGAGCCA GACAGTGAGC TTCTCATGCT GCCCAGAGCA 
AAACGAGGAC GAAGTGCAGA AAGCCTACAC ATGACCAGTG AACTGCAGCC CTCTCTTGAC 
AGGGCTAGGA GTGCTAGTAC CAACTGCTTG AGACCAGATA CTAGTTTGCA TTCACCAGAA 
CGAGAAAGGG GTAGATGGTC CCCCTCCCTA GATAGGAGGC GACCTGCTAG CCCCAGGATT 
CAAATCCAGC ATGCATCTCC GGAGAATGAC AGGCACTCCA GAAAGTCTGA AAGATGTAGC 
ATCCAAAAAC AGTCTAGGAA AGGCACAGCC TCTGATGCAG ACAGGGTTCT CCCACCATGC 
CTTTCTAGAA GGGGATACGC AACCCCAAGA GCAACCGATC AACCGGTCGT TAGGGGAAAG 
CATCCCACTC GTTCACGGTC GAGCGAGCAC TCTAGTGTCA GAACCCTGTG TTCTATGCAC 
CACCTTGCCC CCGGAGGGTC GGCGCCACCT TCTCCACTTC TGACAAGAAC GCACCGACAA 
GGAAGCCCAA CCCAGTCTCC TCCAGCAGAC ACATCCTTCG GCAGTCGCCG TGGAAGACAG 
CTCCCACAGG TGCCAGTTCG AAGCGGCAGT ATAGAACAAG CAAGCTTAGT AGTGGAGGAG 
CGAACGAGAC AGATGAAAAT GAAAGTTCAC CGATTTAAGC AGACAACAGG GTCTGGGTCT 
AGTCAAGAAC TTGACCACGA GCAATACTCC AAGAAGTCAC GTTACATTAT GGAGTACAAC 
ATACATAAAG ATCAGTACAG AAGCTGTGAT AACGCGTCTG CCAAGTCTTC AGATAGTGAT 
GTCAGTGATG TGTCCGCCAT TTCCAGAGCC AGCAGTACCT CACGCCTCAG CAGCACAAGC 
TTTATGTCAG AGCAGTCTGA GCGCCCCAGG GGTAGGATCA GTTCATTTAC CCCCAAAATG 
CAAGGCAGAC GGATGGGGAC TTCAGGAAGA GCCATCATCA AGAGCACCAG TGTAAGTGGA 
GAGATATATA CACTGGAACG TAATGACGGT AGCCAGTCGG ACACGGCCGT GGGTACCGTC 
GGAGCCGGTG GAAAGAAACG AAGATCCAGC CTGAGCGCCA AAGTGGTAGC CATTGTGTCT 
CGAAGAAGCA GGAGCACGTC ACAGCTCAGC CAGACAGAGT CGGGCCACAA GAAGTTGAAA 
AGCACCATCC AGAGGAGTAC GGAAACAGGA ATGGCAGCTG AAATGCGGAA GATGGTGAGA 
CAGCCGAGCC GGGAGTCCAC GGATGGCAGC ATCAACAGTT ATAGCTCGGA AGGAAACTTG 
ATATTTCCTG GAGTTCGAGT AGGACCCGAC AGTCAGTTCA GTGATTTCCT TGATGGGTTG 
GGACCAGCGC AGCTCGTTGG CCGTCAGACG CTCGCCACCC CGGCCATGGG CGATATCCAA 
ATCGGGATGG AGGATAAGAA GGGTCAGTTG GAGGTTGAGG TTATCAGAGC CCGGAGCCTT 
ACACAAAAAC CTGGTTCCAA ATCTACACCC GCTCCCTATG TGAAAGTATA TCTTTTGGAA 
AATGGAGCCT GTATTGCCAA AAAGAAGACA AGAATTGCAC GGAAAACTCT CGATCCTTTG 
TATCAGCAGT CCCTGGTTTT TGATGAAAGT CCACAGGGTA AAGTTCTTCA GGTGATTGTC 
TGGGGTGACT ATGGAAGAAT GGACCACAAA TGCTTTATGG GTGTGGCTCA AATCTTGTTG 
GAAGAACTTG ATCTATCCAG CATGGTGATT GGATGGTATA AATTGTTCCC TCCGTCCTCA 
CTGGTGGATC CCACTCTCGC TCCCCTGACC CGCCGGGCTT CCCAATCATC TCTGGAAAGT 
TCGTCCGGGC CTCCCTGCAT CCGGTCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rims1 ( XM_017596656.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017452145.1 Rattus norvegicus regulating synaptic membrane exocytosis 1 (Rims1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa62873
Clone ID Related Accession (Same CDS sequence)	XM_017596656.1
Accession Version	XM_017596656.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4938bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	regulating synaptic membrane exocytosis protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005108.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCTCGG CCGTGGGGCC CCGAGGTCCT CGCCCACCCA CGGTGCCTCC CCCTATGCAA 
GAACTGCCCG ACCTGAGCCA CCTGACCGAG GAGGAGAGGA ACATTATCAT GGCAGTGATG 
GACCGGCAGA AGGAAGAGGA GGAAAAAGAA GAGGCCATGC TCAAGTGTGT TGTCAGGGAC 
ATGGCGAAGC CTGCTGCCTG CAAAACACCA AGAAATGCTG AAAGCCAGCC CCATCAACCA 
CCACTGAACA TTTTCAGATG TGTCTGTGTT CCCAGAAAGC CAAGCAGCGA AGAGGGAGGC 
CCAGAAAGAG ACTGGAGATT GCATCAACAG TTTGAAAGCT ACAAGGAGCA AGTGAGAAAA 
ATCGGAGAGG AAGCGAGGCG TTACCAGGGC GAGCACAAGG ATGATGCCCC GACGTGTGGA 
ATCTGTCATA AGACAAAGTT TGCTGATGGA TGTGGCCATC TCTGCTCCTA TTGTCGCACC 
AAGTTCTGTG CACGCTGCGG AGGCCGTGTG TCTCTGCGAT CGAACAATGT TATGTGGGTA 
TGCAATTTAT GTCGAAAGCA ACAAGAAATC TTAACGAAAT CTGGAGCGTG GTTCTTTGGA 
AGTGGCCCTC AGCAGCCTAG TCAAGATGGG ACTCTGAGTG ACACGGCCAC AGGTGCTGGA 
TCTGAGGTGC CAAGAGAAAA GAAAGCAAGG CTCCAAGAGC GATCAAGGTC TCAGACGCCC 
TTGAGTACAG CAGCTGTCTC TTCCCAAGAC ACTGCTACCC CCGGTGCACC GTTGGACAGG 
AACAAAGGGG CTGAGCCCTC ACAGCAAGCC TTGGGTCCTG AACAGAAGCA GGCATCAAGA 
TCAAGAAGCG AGCCACCGAG GGAAAGGAAG AAGGCTCCAG GGCTTTCAGA GCAGAATGGC 
AAGGGAGGCC AGAAGAGCGA GCGCAAACGT GTCCCCAAGT CTGTGGTGCA ACCCGGGGAA 
GGGATCGCGG ATGAGAGGGA GAGGAAAGAG AGGCGGGAAA CCCGCAGGTT GGAGAAAGGG 
CGCTCCCAGG ACTACTCAGA CCGGCCTGAG AAACGCGACA ATGGCAGGGT GGCGGAAGAC 
CAGAAGCAGA GGAAGGAGGA GGAGTACCAG ACTAGGTACC GCAGCGACCC TAACCTGGCT 
CGCTACCCGG TGAAGGCGCC GCCAGAGGAG CAGCAGATGC GCATGCACGC CCGGGTGTCC 
CGAGCGAGGC ACGAGCGGCG CCACAGCGAC GTGGCGCTCC CGCACACCGA GGCAGCTGCC 
GCCGCGCCGG CTGAGGCCAC GGCGGGCAAG CGCGCGCCGG CCACCGCCAG GGTCTCTCCC 
CCGGAGTCCC CGCGCGCACG CGCGCCCGCC GCCCAGCCTC CCACCGAGCA CGGGCCACCG 
CCGCCGCGGC CAGTCCCGGG TCCCGCAGAG CCACCCGAGC CGCGCGTCCC CGAGCCGCTC 
CGTAAGCAGG GCCGCCTGGA CCCGGCCTCG GCCGTGCTTC TGCGCAAGGC CAAGCGCGAG 
AAGGCGGAGA GCATGCTGCG GAACGACTCG CTGAGCTCCG ATCAGTCCGA GTCCGTGCGG 
CCATCCCCGC CCAAGCCTCA CCGGCCCAAG CGGGGAGGCA AGAGACGTCA GATGTCGGTG 
AGCAGCTCGG AGGAGGAGGG CGTGTCCACA CCGGAGTACA CGAGCTGCGA GGACGTGGAG 
CTGGAGAGCG AGAGCGTGAG CGAGAAAGGT GACTTGGATT ACTACTGGTT GGATCCCGCC 
ACGTGGCACA GCAGGGAAAC GTCGCCTATC AGTTCGCATC CTGTAACGTG GCAGCCGTCT 
AAAGAGGGAG ATCGACTAAT CGGCCGTGTT ATTCTTAACA AAAGAACAAC CATGCCCAAA 
GAATCAGGTG CATTATTGGG TCTGAAGGTG GTTGGAGGAA AAATGACGGA CTTAGGGCGC 
CTTGGTGCTT TCATCACCAA AGTAAAGAAG GGCAGCCTGG CAGACGTCGT CGGACACCTA 
AGAGCAGGGG ACGAAGTCCT AGAGTGGAAT GGTAAACCCC TGCCGGGAGC AACAAACGAA 
GAAGTTTACA ACATTATCTT AGAATCAAAA TCAGAACCTC AAGTTGAGAT TATTGTTTCA 
AGGCCTATTG GTGACATCCC CAGGATCCCT GAGAGTTCCC ATCCTCCCCT GGAGTCCAGT 
TCAAGTTCCT TTGAATCTCA GAAAATGGAA AGGCCTTCTA TTTCTGTTAT TTCTCCAACC 
AGCCCTGGAG CTCTGAAAGA TGCCCCACAA GTCTTACCAG GGCAACTCTC AGTGAAGCTA 
TGGTATGATA AAGTGGGGCA CCAGCTGATT GTAAATGTTC TACAAGCAAC AGATCTACCC 
CCTAGAGTAG ATGGCCGTCC CAGGAATCCC TATGTAAAAA TGTATTTTCT TCCAGATAGA 
AGCGACAAAA GTAAAAGGAG AACCAAAACA GTAAAGAAAC TTCTAGAGCC AAAATGGAAC 
CAGACATTTG TCTACTCACA CGTACATCGT AGAGATTTTC GAGAGCGAAT GTTAGAGATT 
ACCGTGTGGG ACCAGCCGAG AGTACAGGAC GAAGAGAGTG AATTTCTTGG AGAGATCCTC 
ATAGAGTTGG AAACAGCGCT TTTAGATGAT GAGCCCCATT GGTATAAACT CCAGACACAT 
GACGAATCTT CACTACCTCT GCCTCAGCCA TCACCGTTCA TGCCCAGGCG GCATATTCAT 
GGAGAGAGCT CCAGCAAAAA GCTACAAAGA TCTCAGCGAA TCAGTGATAG TGACATCTCA 
GATTATGAGG TTGATGATGG TATTGGAGTA GTGCCTCCAG TGGGTTATAG AGCTAGTGCT 
AGAGAGAGTA AAGCAACCAC GTTAACAGTG CCAGAGCAAC AAAGAACTAC ACATCACCGC 
TCACGTTCCG TGTCTCCTCA TCGCGGCGAT GATCAGGGAA GGCCTCGTTC ACGTTTACCA 
AATGTGCCAT TACAGAGGAG CTTAGATGAA ATTCATCCAA CACGAAGGTC ACGTTCTCCA 
ACCCGACACC ATGATGCCTC CCGAAGCCCG GCCGATCACA GATCCAGACA TGTGGAAAGT 
CAATATTCGT CAGAGCCAGA CAGTGAGCTT CTCATGCTGC CCAGAGCAAA ACGAGGACGA 
AGTGCAGAAA GCCTACACAT GACCAGTGAA CTGCAGCCCT CTCTTGACAG GGCTAGGAGT 
GCTAGTACCA ACTGCTTGAG ACCAGATACT AGTTTGCATT CACCAGAACG AGAAAGGGGT 
AGATGGTCCC CCTCCCTAGA TAGGAGGCGA CCTGCTAGCC CCAGGATTCA AATCCAGCAT 
GCATCTCCGG AGAATGACAG GCACTCCAGA AAGTCTGAAA GATGTAGCAT CCAAAAACAG 
TCTAGGAAAG GCACAGCCTC TGATGCAGAC AGGGTTCTCC CACCATGCCT TTCTAGAAGG 
GGATACGCAA CCCCAAGAGC AACCGATCAA CCGGTCGTTA GGGGAAAGCA TCCCACTCGT 
TCACGGTCGA GCGAGCACTC TAGTGTCAGA ACCCTGTGTT CTATGCACCA CCTTGCCCCC 
GGAGGGTCGG CGCCACCTTC TCCACTTCTG ACAAGAACGC ACCGACAAGG AAGCCCAACC 
CAGTCTCCTC CAGCAGACAC ATCCTTCGGC AGTCGCCGTG GAAGACAGCT CCCACAGGTG 
CCAGTTCGAA GCGGCAGTAT AGAACAAGCA AGCTTAGTAG TGGAGGAGCG AACGAGACAG 
ATGAAAATGA AAGTTCACCG ATTTAAGCAG ACAACAGGGT CTGGGTCTAG TCAAGAACTT 
GACCACGAGC AATACTCCAA GAAGTCACGT TACATTATGG AGTACAACAT ACATAAAGAT 
CAGTACAGAA GCTGTGATAA CGCGTCTGCC AAGTCTTCAG ATAGTGATGT CAGTGATGTG 
TCCGCCATTT CCAGAGCCAG CAGTACCTCA CGCCTCAGCA GCACAAGCTT TATGTCAGAG 
CAGTCTGAGC GCCCCAGGGG TAGGATCAGT TCATTTACCC CCAAAATGCA AGGCAGACGG 
ATGGGGACTT CAGGAAGAGC CATCATCAAG AGCACCAGTG TAAGTGGAGA GATATATACA 
CTGGAACGTA ATGACGGTAG CCAGTCGGAC ACGGCCGTGG GTACCGTCGG AGCCGGTGGA 
AAGAAACGAA GATCCAGCCT GAGCGCCAAA GTGGTAGCCA TTGTGTCTCG AAGAAGCAGG 
AGCACGTCAC AGCTCAGCCA GACAGAGTCG GGCCACAAGA AGTTGAAAAG CACCATCCAG 
AGGAGTACGG AAACAGGAAT GGCAGCTGAA ATGCGGAAGA TGGTGAGACA GCCGAGCCGG 
GAGTCCACGG ATGGCAGCAT CAACAGTTAT AGCTCGGAAG GAAACTTGAT ATTTCCTGGA 
GTTCGAGTAG GACCCGACAG TCAGTTCAGT GATTTCCTTG ATGGGTTGGG ACCAGCGCAG 
CTCGTTGGCC GTCAGACGCT CGCCACCCCG GCCATGGGCG ATATCCAAAT CGGGATGGAG 
GATAAGAAGG GTCAGTTGGA GGTTGAGGTT ATCAGAGCCC GGAGCCTTAC ACAAAAACCT 
GGTTCCAAAT CTACACCCGC TCCCTATGTG AAAGTATATC TTTTGGAAAA TGGAGCCTGT 
ATTGCCAAAA AGAAGACAAG AATTGCACGG AAAACTCTCG ATCCTTTGTA TCAGCAGTCC 
CTGGTTTTTG ATGAAAGTCC ACAGGGTAAA GTTCTTCAGG TGATTGTCTG GGGTGACTAT 
GGAAGAATGG ACCACAAATG CTTTATGGGT GTGGCTCAAA TCTTGTTGGA AGAACTTGAT 
CTATCCAGCA TGGTGATTGG ATGGTATAAA TTGTTCCCTC CGTCCTCACT GGTGGATCCC 
ACTCTCGCTC CCCTGACCCG CCGGGCTTCC CAATCATCTC TGGAAAGTTC GTCCGGGCCT 
CCCTGCATCC GGTCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017452145.1
CDS	315..5252
Translation	MSSAVGPRGPRPPTVPPPMQELPDLSHLTEEERNIIMAVMDRQKEEEEKEEAMLKCVVRDMAKPAACKTPRNAESQPHQPPLNIFRCVCVPRKPSSEEGGPERDWRLHQQFESYKEQVRKIGEEARRYQGEHKDDAPTCGICHKTKFADGCGHLCSYCRTKFCARCGGRVSLRSNNVMWVCNLCRKQQEILTKSGAWFFGSGPQQPSQDGTLSDTATGAGSEVPREKKARLQERSRSQTPLSTAAVSSQDTATPGAPLDRNKGAEPSQQALGPEQKQASRSRSEPPRERKKAPGLSEQNGKGGQKSERKRVPKSVVQPGEGIADERERKERRETRRLEKGRSQDYSDRPEKRDNGRVAEDQKQRKEEEYQTRYRSDPNLARYPVKAPPEEQQMRMHARVSRARHERRHSDVALPHTEAAAAAPAEATAGKRAPATARVSPPESPRARAPAAQPPTEHGPPPPRPVPGPAEPPEPRVPEPLRKQGRLDPASAVLLRKAKREKAESMLRNDSLSSDQSESVRPSPPKPHRPKRGGKRRQMSVSSSEEEGVSTPEYTSCEDVELESESVSEKGDLDYYWLDPATWHSRETSPISSHPVTWQPSKEGDRLIGRVILNKRTTMPKESGALLGLKVVGGKMTDLGRLGAFITKVKKGSLADVVGHLRAGDEVLEWNGKPLPGATNEEVYNIILESKSEPQVEIIVSRPIGDIPRIPESSHPPLESSSSSFESQKMERPSISVISPTSPGALKDAPQVLPGQLSVKLWYDKVGHQLIVNVLQATDLPPRVDGRPRNPYVKMYFLPDRSDKSKRRTKTVKKLLEPKWNQTFVYSHVHRRDFRERMLEITVWDQPRVQDEESEFLGEILIELETALLDDEPHWYKLQTHDESSLPLPQPSPFMPRRHIHGESSSKKLQRSQRISDSDISDYEVDDGIGVVPPVGYRASARESKATTLTVPEQQRTTHHRSRSVSPHRGDDQGRPRSRLPNVPLQRSLDEIHPTRRSRSPTRHHDASRSPADHRSRHVESQYSSEPDSELLMLPRAKRGRSAESLHMTSELQPSLDRARSASTNCLRPDTSLHSPERERGRWSPSLDRRRPASPRIQIQHASPENDRHSRKSERCSIQKQSRKGTASDADRVLPPCLSRRGYATPRATDQPVVRGKHPTRSRSSEHSSVRTLCSMHHLAPGGSAPPSPLLTRTHRQGSPTQSPPADTSFGSRRGRQLPQVPVRSGSIEQASLVVEERTRQMKMKVHRFKQTTGSGSSQELDHEQYSKKSRYIMEYNIHKDQYRSCDNASAKSSDSDVSDVSAISRASSTSRLSSTSFMSEQSERPRGRISSFTPKMQGRRMGTSGRAIIKSTSVSGEIYTLERNDGSQSDTAVGTVGAGGKKRRSSLSAKVVAIVSRRSRSTSQLSQTESGHKKLKSTIQRSTETGMAAEMRKMVRQPSRESTDGSINSYSSEGNLIFPGVRVGPDSQFSDFLDGLGPAQLVGRQTLATPAMGDIQIGMEDKKGQLEVEVIRARSLTQKPGSKSTPAPYVKVYLLENGACIAKKKTRIARKTLDPLYQQSLVFDESPQGKVLQVIVWGDYGRMDHKCFMGVAQILLEELDLSSMVIGWYKLFPPSSLVDPTLAPLTRRASQSSLESSSGPPCIRS

Target ORF information:

RefSeq Version	XM_017596656.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus regulating synaptic membrane exocytosis 1 (Rims1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017596656.1

ORF Insert Sequence:

ATGTCCTCGG CCGTGGGGCC CCGAGGTCCT CGCCCACCCA CGGTGCCTCC CCCTATGCAA 
GAACTGCCCG ACCTGAGCCA CCTGACCGAG GAGGAGAGGA ACATTATCAT GGCAGTGATG 
GACCGGCAGA AGGAAGAGGA GGAAAAAGAA GAGGCCATGC TCAAGTGTGT TGTCAGGGAC 
ATGGCGAAGC CTGCTGCCTG CAAAACACCA AGAAATGCTG AAAGCCAGCC CCATCAACCA 
CCACTGAACA TTTTCAGATG TGTCTGTGTT CCCAGAAAGC CAAGCAGCGA AGAGGGAGGC 
CCAGAAAGAG ACTGGAGATT GCATCAACAG TTTGAAAGCT ACAAGGAGCA AGTGAGAAAA 
ATCGGAGAGG AAGCGAGGCG TTACCAGGGC GAGCACAAGG ATGATGCCCC GACGTGTGGA 
ATCTGTCATA AGACAAAGTT TGCTGATGGA TGTGGCCATC TCTGCTCCTA TTGTCGCACC 
AAGTTCTGTG CACGCTGCGG AGGCCGTGTG TCTCTGCGAT CGAACAATGT TATGTGGGTA 
TGCAATTTAT GTCGAAAGCA ACAAGAAATC TTAACGAAAT CTGGAGCGTG GTTCTTTGGA 
AGTGGCCCTC AGCAGCCTAG TCAAGATGGG ACTCTGAGTG ACACGGCCAC AGGTGCTGGA 
TCTGAGGTGC CAAGAGAAAA GAAAGCAAGG CTCCAAGAGC GATCAAGGTC TCAGACGCCC 
TTGAGTACAG CAGCTGTCTC TTCCCAAGAC ACTGCTACCC CCGGTGCACC GTTGGACAGG 
AACAAAGGGG CTGAGCCCTC ACAGCAAGCC TTGGGTCCTG AACAGAAGCA GGCATCAAGA 
TCAAGAAGCG AGCCACCGAG GGAAAGGAAG AAGGCTCCAG GGCTTTCAGA GCAGAATGGC 
AAGGGAGGCC AGAAGAGCGA GCGCAAACGT GTCCCCAAGT CTGTGGTGCA ACCCGGGGAA 
GGGATCGCGG ATGAGAGGGA GAGGAAAGAG AGGCGGGAAA CCCGCAGGTT GGAGAAAGGG 
CGCTCCCAGG ACTACTCAGA CCGGCCTGAG AAACGCGACA ATGGCAGGGT GGCGGAAGAC 
CAGAAGCAGA GGAAGGAGGA GGAGTACCAG ACTAGGTACC GCAGCGACCC TAACCTGGCT 
CGCTACCCGG TGAAGGCGCC GCCAGAGGAG CAGCAGATGC GCATGCACGC CCGGGTGTCC 
CGAGCGAGGC ACGAGCGGCG CCACAGCGAC GTGGCGCTCC CGCACACCGA GGCAGCTGCC 
GCCGCGCCGG CTGAGGCCAC GGCGGGCAAG CGCGCGCCGG CCACCGCCAG GGTCTCTCCC 
CCGGAGTCCC CGCGCGCACG CGCGCCCGCC GCCCAGCCTC CCACCGAGCA CGGGCCACCG 
CCGCCGCGGC CAGTCCCGGG TCCCGCAGAG CCACCCGAGC CGCGCGTCCC CGAGCCGCTC 
CGTAAGCAGG GCCGCCTGGA CCCGGCCTCG GCCGTGCTTC TGCGCAAGGC CAAGCGCGAG 
AAGGCGGAGA GCATGCTGCG GAACGACTCG CTGAGCTCCG ATCAGTCCGA GTCCGTGCGG 
CCATCCCCGC CCAAGCCTCA CCGGCCCAAG CGGGGAGGCA AGAGACGTCA GATGTCGGTG 
AGCAGCTCGG AGGAGGAGGG CGTGTCCACA CCGGAGTACA CGAGCTGCGA GGACGTGGAG 
CTGGAGAGCG AGAGCGTGAG CGAGAAAGGT GACTTGGATT ACTACTGGTT GGATCCCGCC 
ACGTGGCACA GCAGGGAAAC GTCGCCTATC AGTTCGCATC CTGTAACGTG GCAGCCGTCT 
AAAGAGGGAG ATCGACTAAT CGGCCGTGTT ATTCTTAACA AAAGAACAAC CATGCCCAAA 
GAATCAGGTG CATTATTGGG TCTGAAGGTG GTTGGAGGAA AAATGACGGA CTTAGGGCGC 
CTTGGTGCTT TCATCACCAA AGTAAAGAAG GGCAGCCTGG CAGACGTCGT CGGACACCTA 
AGAGCAGGGG ACGAAGTCCT AGAGTGGAAT GGTAAACCCC TGCCGGGAGC AACAAACGAA 
GAAGTTTACA ACATTATCTT AGAATCAAAA TCAGAACCTC AAGTTGAGAT TATTGTTTCA 
AGGCCTATTG GTGACATCCC CAGGATCCCT GAGAGTTCCC ATCCTCCCCT GGAGTCCAGT 
TCAAGTTCCT TTGAATCTCA GAAAATGGAA AGGCCTTCTA TTTCTGTTAT TTCTCCAACC 
AGCCCTGGAG CTCTGAAAGA TGCCCCACAA GTCTTACCAG GGCAACTCTC AGTGAAGCTA 
TGGTATGATA AAGTGGGGCA CCAGCTGATT GTAAATGTTC TACAAGCAAC AGATCTACCC 
CCTAGAGTAG ATGGCCGTCC CAGGAATCCC TATGTAAAAA TGTATTTTCT TCCAGATAGA 
AGCGACAAAA GTAAAAGGAG AACCAAAACA GTAAAGAAAC TTCTAGAGCC AAAATGGAAC 
CAGACATTTG TCTACTCACA CGTACATCGT AGAGATTTTC GAGAGCGAAT GTTAGAGATT 
ACCGTGTGGG ACCAGCCGAG AGTACAGGAC GAAGAGAGTG AATTTCTTGG AGAGATCCTC 
ATAGAGTTGG AAACAGCGCT TTTAGATGAT GAGCCCCATT GGTATAAACT CCAGACACAT 
GACGAATCTT CACTACCTCT GCCTCAGCCA TCACCGTTCA TGCCCAGGCG GCATATTCAT 
GGAGAGAGCT CCAGCAAAAA GCTACAAAGA TCTCAGCGAA TCAGTGATAG TGACATCTCA 
GATTATGAGG TTGATGATGG TATTGGAGTA GTGCCTCCAG TGGGTTATAG AGCTAGTGCT 
AGAGAGAGTA AAGCAACCAC GTTAACAGTG CCAGAGCAAC AAAGAACTAC ACATCACCGC 
TCACGTTCCG TGTCTCCTCA TCGCGGCGAT GATCAGGGAA GGCCTCGTTC ACGTTTACCA 
AATGTGCCAT TACAGAGGAG CTTAGATGAA ATTCATCCAA CACGAAGGTC ACGTTCTCCA 
ACCCGACACC ATGATGCCTC CCGAAGCCCG GCCGATCACA GATCCAGACA TGTGGAAAGT 
CAATATTCGT CAGAGCCAGA CAGTGAGCTT CTCATGCTGC CCAGAGCAAA ACGAGGACGA 
AGTGCAGAAA GCCTACACAT GACCAGTGAA CTGCAGCCCT CTCTTGACAG GGCTAGGAGT 
GCTAGTACCA ACTGCTTGAG ACCAGATACT AGTTTGCATT CACCAGAACG AGAAAGGGGT 
AGATGGTCCC CCTCCCTAGA TAGGAGGCGA CCTGCTAGCC CCAGGATTCA AATCCAGCAT 
GCATCTCCGG AGAATGACAG GCACTCCAGA AAGTCTGAAA GATGTAGCAT CCAAAAACAG 
TCTAGGAAAG GCACAGCCTC TGATGCAGAC AGGGTTCTCC CACCATGCCT TTCTAGAAGG 
GGATACGCAA CCCCAAGAGC AACCGATCAA CCGGTCGTTA GGGGAAAGCA TCCCACTCGT 
TCACGGTCGA GCGAGCACTC TAGTGTCAGA ACCCTGTGTT CTATGCACCA CCTTGCCCCC 
GGAGGGTCGG CGCCACCTTC TCCACTTCTG ACAAGAACGC ACCGACAAGG AAGCCCAACC 
CAGTCTCCTC CAGCAGACAC ATCCTTCGGC AGTCGCCGTG GAAGACAGCT CCCACAGGTG 
CCAGTTCGAA GCGGCAGTAT AGAACAAGCA AGCTTAGTAG TGGAGGAGCG AACGAGACAG 
ATGAAAATGA AAGTTCACCG ATTTAAGCAG ACAACAGGGT CTGGGTCTAG TCAAGAACTT 
GACCACGAGC AATACTCCAA GAAGTCACGT TACATTATGG AGTACAACAT ACATAAAGAT 
CAGTACAGAA GCTGTGATAA CGCGTCTGCC AAGTCTTCAG ATAGTGATGT CAGTGATGTG 
TCCGCCATTT CCAGAGCCAG CAGTACCTCA CGCCTCAGCA GCACAAGCTT TATGTCAGAG 
CAGTCTGAGC GCCCCAGGGG TAGGATCAGT TCATTTACCC CCAAAATGCA AGGCAGACGG 
ATGGGGACTT CAGGAAGAGC CATCATCAAG AGCACCAGTG TAAGTGGAGA GATATATACA 
CTGGAACGTA ATGACGGTAG CCAGTCGGAC ACGGCCGTGG GTACCGTCGG AGCCGGTGGA 
AAGAAACGAA GATCCAGCCT GAGCGCCAAA GTGGTAGCCA TTGTGTCTCG AAGAAGCAGG 
AGCACGTCAC AGCTCAGCCA GACAGAGTCG GGCCACAAGA AGTTGAAAAG CACCATCCAG 
AGGAGTACGG AAACAGGAAT GGCAGCTGAA ATGCGGAAGA TGGTGAGACA GCCGAGCCGG 
GAGTCCACGG ATGGCAGCAT CAACAGTTAT AGCTCGGAAG GAAACTTGAT ATTTCCTGGA 
GTTCGAGTAG GACCCGACAG TCAGTTCAGT GATTTCCTTG ATGGGTTGGG ACCAGCGCAG 
CTCGTTGGCC GTCAGACGCT CGCCACCCCG GCCATGGGCG ATATCCAAAT CGGGATGGAG 
GATAAGAAGG GTCAGTTGGA GGTTGAGGTT ATCAGAGCCC GGAGCCTTAC ACAAAAACCT 
GGTTCCAAAT CTACACCCGC TCCCTATGTG AAAGTATATC TTTTGGAAAA TGGAGCCTGT 
ATTGCCAAAA AGAAGACAAG AATTGCACGG AAAACTCTCG ATCCTTTGTA TCAGCAGTCC 
CTGGTTTTTG ATGAAAGTCC ACAGGGTAAA GTTCTTCAGG TGATTGTCTG GGGTGACTAT 
GGAAGAATGG ACCACAAATG CTTTATGGGT GTGGCTCAAA TCTTGTTGGA AGAACTTGAT 
CTATCCAGCA TGGTGATTGG ATGGTATAAA TTGTTCCCTC CGTCCTCACT GGTGGATCCC 
ACTCTCGCTC CCCTGACCCG CCGGGCTTCC CAATCATCTC TGGAAAGTTC GTCCGGGCCT 
CCCTGCATCC GGTCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rims1 ( XM_017596657.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017452146.1 Rattus norvegicus regulating synaptic membrane exocytosis 1 (Rims1), transcript variant X3, mRNA.

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CloneID	ORa62874
Clone ID Related Accession (Same CDS sequence)	XM_017596657.1
Accession Version	XM_017596657.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4929bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	regulating synaptic membrane exocytosis protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005108.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCTCGG CCGTGGGGCC CCGAGGTCCT CGCCCACCCA CGGTGCCTCC CCCTATGCAA 
GAACTGCCCG ACCTGAGCCA CCTGACCGAG GAGGAGAGGA ACATTATCAT GGCAGTGATG 
GACCGGCAGA AGGAAGAGGA GGAAAAAGAA GAGGCCATGC TCAAGTGTGT TGTCAGGGAC 
ATGGCGAAGC CTGCTGCCTG CAAAACACCA AGAAATGCTG AAAGCCAGCC CCATCAACCA 
CCACTGAACA TTTTCAGATG TGTCTGTGTT CCCAGAAAGC CAAGCAGCGA AGAGGGAGGC 
CCAGAAAGAG ACTGGAGATT GCATCAACAG TTTGAAAGCT ACAAGGAGCA AGTGAGAAAA 
ATCGGAGAGG AAGCGAGGCG TTACCAGGGC GAGCACAAGG ATGATGCCCC GACGTGTGGA 
ATCTGTCATA AGACAAAGTT TGCTGATGGA TGTGGCCATC TCTGCTCCTA TTGTCGCACC 
AAGTTCTGTG CACGCTGCGG AGGCCGTGTG TCTCTGCGAT CGAACAATGA GGACAAAGTG 
GTTATGTGGG TATGCAATTT ATGTCGAAAG CAACAAGAAA TCTTAACGAA ATCTGGAGCG 
TGGTTCTTTG GAAGTGGCCC TCAGCAGCCT AGTCAAGATG GGACTCTGAG TGACACGGCC 
ACAGGTGCTG GATCTGAGGT GCCAAGAGAA AAGAAAGCAA GGCTCCAAGA GCGATCAAGG 
TCTCAGACGC CCTTGAGTAC AGCAGCTGTC TCTTCCCAAG ACACTGCTAC CCCCGGTGCA 
CCGTTGGACA GGAACAAAGG GGCTGAGCCC TCACAGCAAG CCTTGGGTCC TGAACAGAAG 
CAGGCATCAA GATCAAGAAG CGAGCCACCG AGGGAAAGGA AGAAGGCTCC AGGGCTTTCA 
GAGCAGAATG GCAAGGGAGG CCAGAAGAGC GAGCGCAAAC GTGTCCCCAA GTCTGTGGTG 
CAACCCGGGG AAGGGATCGC GGATGAGAGG GAGAGGAAAG AGAGGCGGGA AACCCGCAGG 
TTGGAGAAAG GGCGCTCCCA GGACTACTCA GACCGGCCTG AGAAACGCGA CAATGGCAGG 
GTGGCGGAAG ACCAGAAGCA GAGGAAGGAG GAGGAGTACC AGACTAGGTA CCGCAGCGAC 
CCTAACCTGG CTCGCTACCC GGTGAAGGCG CCGCCAGAGG AGCAGCAGAT GCGCATGCAC 
GCCCGGGTGT CCCGAGCGAG GCACGAGCGG CGCCACAGCG ACGTGGCGCT CCCGCACACC 
GAGGCAGCTG CCGCCGCGCC GGCTGAGGCC ACGGCGGGCA AGCGCGCGCC GGCCACCGCC 
AGGGTCTCTC CCCCGGAGTC CCCGCGCGCA CGCGCGCCCG CCGCCCAGCC TCCCACCGAG 
CACGGGCCAC CGCCGCCGCG GCCAGTCCCG GGTCCCGCAG AGCCACCCGA GCCGCGCGTC 
CCCGAGCCGC TCCGTAAGCA GGGCCGCCTG GACCCGGCCT CGGCCGTGCT TCTGCGCAAG 
GCCAAGCGCG AGAAGGCGGA GAGCATGCTG CGGAACGACT CGCTGAGCTC CGATCAGTCC 
GAGTCCGTGC GGCCATCCCC GCCCAAGCCT CACCGGCCCA AGCGGGGAGG CAAGAGACGT 
CAGATGTCGG TGAGCAGCTC GGAGGAGGAG GGCGTGTCCA CACCGGAGTA CACGAGCTGC 
GAGGACGTGG AGCTGGAGAG CGAGAGCGTG AGCGAGAAAG GTGACTTGGA TTACTACTGG 
TTGGATCCCG CCACGTGGCA CAGCAGGGAA ACGTCGCCTA TCAGTTCGCA TCCTGTAACG 
TGGCAGCCGT CTAAAGAGGG AGATCGACTA ATCGGCCGTG TTATTCTTAA CAAAAGAACA 
ACCATGCCCA AAGAATCAGG TGCATTATTG GGTCTGAAGG TGGTTGGAGG AAAAATGACG 
GACTTAGGGC GCCTTGGTGC TTTCATCACC AAAGTAAAGA AGGGCAGCCT GGCAGACGTC 
GTCGGACACC TAAGAGCAGG GGACGAAGTC CTAGAGTGGA ATGGTAAACC CCTGCCGGGA 
GCAACAAACG AAGAAGTTTA CAACATTATC TTAGAATCAA AATCAGAACC TCAAGTTGAG 
ATTATTGTTT CAAGGCCTAT TGGTGACATC CCCAGGATCC CTGAGAGTTC CCATCCTCCC 
CTGGAGTCCA GTTCAAGTTC CTTTGAATCT CAGAAAATGG AAAGGCCTTC TATTTCTGTT 
ATTTCTCCAA CCAGCCCTGG AGCTCTGAAA GATGCCCCAC AAGTCTTACC AGGGCAACTC 
TCAGTGAAGC TATGGTATGA TAAAGTGGGG CACCAGCTGA TTGTAAATGT TCTACAAGCA 
ACAGATCTAC CCCCTAGAGT AGATGGCCGT CCCAGGAATC CCTATGTAAA AATGTATTTT 
CTTCCAGATA GAAGCGACAA AAGTAAAAGG AGAACCAAAA CAGTAAAGAA ACTTCTAGAG 
CCAAAATGGA ACCAGACATT TGTCTACTCA CACGTACATC GTAGAGATTT TCGAGAGCGA 
ATGTTAGAGA TTACCGTGTG GGACCAGCCG AGAGTACAGG ACGAAGAGAG TGAATTTCTT 
GGAGAGATCC TCATAGAGTT GGAAACAGCG CTTTTAGATG ATGAGCCCCA TTGGTATAAA 
CTCCAGACAC ATGACGAATC TTCACTACCT CTGCCTCAGC CATCACCGTT CATGCCCAGG 
CGGCATATTC ATGGAGAGAG CTCCAGCAAA AAGCTACAAA GATCTCAGCG AATCAGTGAT 
AGTGACATCT CAGATTATGA GGTTGATGAT GGTATTGGAG TAGTGCCTCC AGTGGGTTAT 
AGAGCTAGTG CTAGAGAGAG TAAAGCAACC ACGTTAACAG TGCCAGAGCA ACAAAGAACT 
ACACATCACC GCTCACGTTC CGTGTCTCCT CATCGCGGCG ATGATCAGGG AAGGCCTCGT 
TCACGTTTAC CAAATGTGCC ATTACAGAGG AGCTTAGATG AAATTCATCC AACACGAAGG 
TCACGTTCTC CAACCCGACA CCATGATGCC TCCCGAAGCC CGGCCGATCA CAGATCCAGA 
CATGTGGAAA GTCAATATTC GTCAGAGCCA GACAGTGAGC TTCTCATGCT GCCCAGAGCA 
AAACGAGGAC GAAGTGCAGA AAGCCTACAC ATGACCAGTG AACTGCAGCC CTCTCTTGAC 
AGGGCTAGGA GTGCTAGTAC CAACTGCTTG AGACCAGATA CTAGTTTGCA TTCACCAGAA 
CGAGAAAGGG GTAGATGGTC CCCCTCCCTA GATAGGAGGC GACCTGCTAG CCCCAGGATT 
CAAATCCAGC ATGCATCTCC GGAGAATGAC AGGCACTCCA GAAAGTCTGA AAGATGTAGC 
ATCCAAAAAC AGTCTAGGAA AGGCACAGCC TCTGATGCAG ACAGGGTTCT CCCACCATGC 
CTTTCTAGAA GGGGATACGC AACCCCAAGA GCAACCGATC AACCGGTCGT TAGGGGAAAG 
CATCCCACTC GTTCACGGTC GAGCGAGCAC TCTAGTGTCA GAACCCTGTG TTCTATGCAC 
CACCTTGCCC CCGGAGGGTC GGCGCCACCT TCTCCACTTC TGACAAGAAC GCACCGACAA 
GGAAGCCCAA CCCAGTCTCC TCCAGCAGAC ACATCCTTCG GCAGTCGCCG TGGAAGACAG 
CTCCCACAGG TGCCAGTTCG AAGCGGCAGT ATAGAACAAG CAAGCTTAGT AGTGGAGGAG 
CGAACGAGAC AGATGAAAAT GAAAGTTCAC CGATTTAAGC AGACAACAGG GTCTGGGTCT 
AGTCAAGAAC TTGACCACGA GCAATACTCC AAGTACAACA TACATAAAGA TCAGTACAGA 
AGCTGTGATA ACGCGTCTGC CAAGTCTTCA GATAGTGATG TCAGTGATGT GTCCGCCATT 
TCCAGAGCCA GCAGTACCTC ACGCCTCAGC AGCACAAGCT TTATGTCAGA GCAGTCTGAG 
CGCCCCAGGG GTAGGATCAG TTCATTTACC CCCAAAATGC AAGGCAGACG GATGGGGACT 
TCAGGAAGAG CCATCATCAA GAGCACCAGT GTAAGTGGAG AGATATATAC ACTGGAACGT 
AATGACGGTA GCCAGTCGGA CACGGCCGTG GGTACCGTCG GAGCCGGTGG AAAGAAACGA 
AGATCCAGCC TGAGCGCCAA AGTGGTAGCC ATTGTGTCTC GAAGAAGCAG GAGCACGTCA 
CAGCTCAGCC AGACAGAGTC GGGCCACAAG AAGTTGAAAA GCACCATCCA GAGGAGTACG 
GAAACAGGAA TGGCAGCTGA AATGCGGAAG ATGGTGAGAC AGCCGAGCCG GGAGTCCACG 
GATGGCAGCA TCAACAGTTA TAGCTCGGAA GGAAACTTGA TATTTCCTGG AGTTCGAGTA 
GGACCCGACA GTCAGTTCAG TGATTTCCTT GATGGGTTGG GACCAGCGCA GCTCGTTGGC 
CGTCAGACGC TCGCCACCCC GGCCATGGGC GATATCCAAA TCGGGATGGA GGATAAGAAG 
GGTCAGTTGG AGGTTGAGGT TATCAGAGCC CGGAGCCTTA CACAAAAACC TGGTTCCAAA 
TCTACACCCG CTCCCTATGT GAAAGTATAT CTTTTGGAAA ATGGAGCCTG TATTGCCAAA 
AAGAAGACAA GAATTGCACG GAAAACTCTC GATCCTTTGT ATCAGCAGTC CCTGGTTTTT 
GATGAAAGTC CACAGGGTAA AGTTCTTCAG GTGATTGTCT GGGGTGACTA TGGAAGAATG 
GACCACAAAT GCTTTATGGG TGTGGCTCAA ATCTTGTTGG AAGAACTTGA TCTATCCAGC 
ATGGTGATTG GATGGTATAA ATTGTTCCCT CCGTCCTCAC TGGTGGATCC CACTCTCGCT 
CCCCTGACCC GCCGGGCTTC CCAATCATCT CTGGAAAGTT CGTCCGGGCC TCCCTGCATC 
CGGTCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017452146.1
CDS	314..5242
Translation	MSSAVGPRGPRPPTVPPPMQELPDLSHLTEEERNIIMAVMDRQKEEEEKEEAMLKCVVRDMAKPAACKTPRNAESQPHQPPLNIFRCVCVPRKPSSEEGGPERDWRLHQQFESYKEQVRKIGEEARRYQGEHKDDAPTCGICHKTKFADGCGHLCSYCRTKFCARCGGRVSLRSNNEDKVVMWVCNLCRKQQEILTKSGAWFFGSGPQQPSQDGTLSDTATGAGSEVPREKKARLQERSRSQTPLSTAAVSSQDTATPGAPLDRNKGAEPSQQALGPEQKQASRSRSEPPRERKKAPGLSEQNGKGGQKSERKRVPKSVVQPGEGIADERERKERRETRRLEKGRSQDYSDRPEKRDNGRVAEDQKQRKEEEYQTRYRSDPNLARYPVKAPPEEQQMRMHARVSRARHERRHSDVALPHTEAAAAAPAEATAGKRAPATARVSPPESPRARAPAAQPPTEHGPPPPRPVPGPAEPPEPRVPEPLRKQGRLDPASAVLLRKAKREKAESMLRNDSLSSDQSESVRPSPPKPHRPKRGGKRRQMSVSSSEEEGVSTPEYTSCEDVELESESVSEKGDLDYYWLDPATWHSRETSPISSHPVTWQPSKEGDRLIGRVILNKRTTMPKESGALLGLKVVGGKMTDLGRLGAFITKVKKGSLADVVGHLRAGDEVLEWNGKPLPGATNEEVYNIILESKSEPQVEIIVSRPIGDIPRIPESSHPPLESSSSSFESQKMERPSISVISPTSPGALKDAPQVLPGQLSVKLWYDKVGHQLIVNVLQATDLPPRVDGRPRNPYVKMYFLPDRSDKSKRRTKTVKKLLEPKWNQTFVYSHVHRRDFRERMLEITVWDQPRVQDEESEFLGEILIELETALLDDEPHWYKLQTHDESSLPLPQPSPFMPRRHIHGESSSKKLQRSQRISDSDISDYEVDDGIGVVPPVGYRASARESKATTLTVPEQQRTTHHRSRSVSPHRGDDQGRPRSRLPNVPLQRSLDEIHPTRRSRSPTRHHDASRSPADHRSRHVESQYSSEPDSELLMLPRAKRGRSAESLHMTSELQPSLDRARSASTNCLRPDTSLHSPERERGRWSPSLDRRRPASPRIQIQHASPENDRHSRKSERCSIQKQSRKGTASDADRVLPPCLSRRGYATPRATDQPVVRGKHPTRSRSSEHSSVRTLCSMHHLAPGGSAPPSPLLTRTHRQGSPTQSPPADTSFGSRRGRQLPQVPVRSGSIEQASLVVEERTRQMKMKVHRFKQTTGSGSSQELDHEQYSKYNIHKDQYRSCDNASAKSSDSDVSDVSAISRASSTSRLSSTSFMSEQSERPRGRISSFTPKMQGRRMGTSGRAIIKSTSVSGEIYTLERNDGSQSDTAVGTVGAGGKKRRSSLSAKVVAIVSRRSRSTSQLSQTESGHKKLKSTIQRSTETGMAAEMRKMVRQPSRESTDGSINSYSSEGNLIFPGVRVGPDSQFSDFLDGLGPAQLVGRQTLATPAMGDIQIGMEDKKGQLEVEVIRARSLTQKPGSKSTPAPYVKVYLLENGACIAKKKTRIARKTLDPLYQQSLVFDESPQGKVLQVIVWGDYGRMDHKCFMGVAQILLEELDLSSMVIGWYKLFPPSSLVDPTLAPLTRRASQSSLESSSGPPCIRS

Target ORF information:

RefSeq Version	XM_017596657.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus regulating synaptic membrane exocytosis 1 (Rims1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017596657.1

ORF Insert Sequence:

ATGTCCTCGG CCGTGGGGCC CCGAGGTCCT CGCCCACCCA CGGTGCCTCC CCCTATGCAA 
GAACTGCCCG ACCTGAGCCA CCTGACCGAG GAGGAGAGGA ACATTATCAT GGCAGTGATG 
GACCGGCAGA AGGAAGAGGA GGAAAAAGAA GAGGCCATGC TCAAGTGTGT TGTCAGGGAC 
ATGGCGAAGC CTGCTGCCTG CAAAACACCA AGAAATGCTG AAAGCCAGCC CCATCAACCA 
CCACTGAACA TTTTCAGATG TGTCTGTGTT CCCAGAAAGC CAAGCAGCGA AGAGGGAGGC 
CCAGAAAGAG ACTGGAGATT GCATCAACAG TTTGAAAGCT ACAAGGAGCA AGTGAGAAAA 
ATCGGAGAGG AAGCGAGGCG TTACCAGGGC GAGCACAAGG ATGATGCCCC GACGTGTGGA 
ATCTGTCATA AGACAAAGTT TGCTGATGGA TGTGGCCATC TCTGCTCCTA TTGTCGCACC 
AAGTTCTGTG CACGCTGCGG AGGCCGTGTG TCTCTGCGAT CGAACAATGA GGACAAAGTG 
GTTATGTGGG TATGCAATTT ATGTCGAAAG CAACAAGAAA TCTTAACGAA ATCTGGAGCG 
TGGTTCTTTG GAAGTGGCCC TCAGCAGCCT AGTCAAGATG GGACTCTGAG TGACACGGCC 
ACAGGTGCTG GATCTGAGGT GCCAAGAGAA AAGAAAGCAA GGCTCCAAGA GCGATCAAGG 
TCTCAGACGC CCTTGAGTAC AGCAGCTGTC TCTTCCCAAG ACACTGCTAC CCCCGGTGCA 
CCGTTGGACA GGAACAAAGG GGCTGAGCCC TCACAGCAAG CCTTGGGTCC TGAACAGAAG 
CAGGCATCAA GATCAAGAAG CGAGCCACCG AGGGAAAGGA AGAAGGCTCC AGGGCTTTCA 
GAGCAGAATG GCAAGGGAGG CCAGAAGAGC GAGCGCAAAC GTGTCCCCAA GTCTGTGGTG 
CAACCCGGGG AAGGGATCGC GGATGAGAGG GAGAGGAAAG AGAGGCGGGA AACCCGCAGG 
TTGGAGAAAG GGCGCTCCCA GGACTACTCA GACCGGCCTG AGAAACGCGA CAATGGCAGG 
GTGGCGGAAG ACCAGAAGCA GAGGAAGGAG GAGGAGTACC AGACTAGGTA CCGCAGCGAC 
CCTAACCTGG CTCGCTACCC GGTGAAGGCG CCGCCAGAGG AGCAGCAGAT GCGCATGCAC 
GCCCGGGTGT CCCGAGCGAG GCACGAGCGG CGCCACAGCG ACGTGGCGCT CCCGCACACC 
GAGGCAGCTG CCGCCGCGCC GGCTGAGGCC ACGGCGGGCA AGCGCGCGCC GGCCACCGCC 
AGGGTCTCTC CCCCGGAGTC CCCGCGCGCA CGCGCGCCCG CCGCCCAGCC TCCCACCGAG 
CACGGGCCAC CGCCGCCGCG GCCAGTCCCG GGTCCCGCAG AGCCACCCGA GCCGCGCGTC 
CCCGAGCCGC TCCGTAAGCA GGGCCGCCTG GACCCGGCCT CGGCCGTGCT TCTGCGCAAG 
GCCAAGCGCG AGAAGGCGGA GAGCATGCTG CGGAACGACT CGCTGAGCTC CGATCAGTCC 
GAGTCCGTGC GGCCATCCCC GCCCAAGCCT CACCGGCCCA AGCGGGGAGG CAAGAGACGT 
CAGATGTCGG TGAGCAGCTC GGAGGAGGAG GGCGTGTCCA CACCGGAGTA CACGAGCTGC 
GAGGACGTGG AGCTGGAGAG CGAGAGCGTG AGCGAGAAAG GTGACTTGGA TTACTACTGG 
TTGGATCCCG CCACGTGGCA CAGCAGGGAA ACGTCGCCTA TCAGTTCGCA TCCTGTAACG 
TGGCAGCCGT CTAAAGAGGG AGATCGACTA ATCGGCCGTG TTATTCTTAA CAAAAGAACA 
ACCATGCCCA AAGAATCAGG TGCATTATTG GGTCTGAAGG TGGTTGGAGG AAAAATGACG 
GACTTAGGGC GCCTTGGTGC TTTCATCACC AAAGTAAAGA AGGGCAGCCT GGCAGACGTC 
GTCGGACACC TAAGAGCAGG GGACGAAGTC CTAGAGTGGA ATGGTAAACC CCTGCCGGGA 
GCAACAAACG AAGAAGTTTA CAACATTATC TTAGAATCAA AATCAGAACC TCAAGTTGAG 
ATTATTGTTT CAAGGCCTAT TGGTGACATC CCCAGGATCC CTGAGAGTTC CCATCCTCCC 
CTGGAGTCCA GTTCAAGTTC CTTTGAATCT CAGAAAATGG AAAGGCCTTC TATTTCTGTT 
ATTTCTCCAA CCAGCCCTGG AGCTCTGAAA GATGCCCCAC AAGTCTTACC AGGGCAACTC 
TCAGTGAAGC TATGGTATGA TAAAGTGGGG CACCAGCTGA TTGTAAATGT TCTACAAGCA 
ACAGATCTAC CCCCTAGAGT AGATGGCCGT CCCAGGAATC CCTATGTAAA AATGTATTTT 
CTTCCAGATA GAAGCGACAA AAGTAAAAGG AGAACCAAAA CAGTAAAGAA ACTTCTAGAG 
CCAAAATGGA ACCAGACATT TGTCTACTCA CACGTACATC GTAGAGATTT TCGAGAGCGA 
ATGTTAGAGA TTACCGTGTG GGACCAGCCG AGAGTACAGG ACGAAGAGAG TGAATTTCTT 
GGAGAGATCC TCATAGAGTT GGAAACAGCG CTTTTAGATG ATGAGCCCCA TTGGTATAAA 
CTCCAGACAC ATGACGAATC TTCACTACCT CTGCCTCAGC CATCACCGTT CATGCCCAGG 
CGGCATATTC ATGGAGAGAG CTCCAGCAAA AAGCTACAAA GATCTCAGCG AATCAGTGAT 
AGTGACATCT CAGATTATGA GGTTGATGAT GGTATTGGAG TAGTGCCTCC AGTGGGTTAT 
AGAGCTAGTG CTAGAGAGAG TAAAGCAACC ACGTTAACAG TGCCAGAGCA ACAAAGAACT 
ACACATCACC GCTCACGTTC CGTGTCTCCT CATCGCGGCG ATGATCAGGG AAGGCCTCGT 
TCACGTTTAC CAAATGTGCC ATTACAGAGG AGCTTAGATG AAATTCATCC AACACGAAGG 
TCACGTTCTC CAACCCGACA CCATGATGCC TCCCGAAGCC CGGCCGATCA CAGATCCAGA 
CATGTGGAAA GTCAATATTC GTCAGAGCCA GACAGTGAGC TTCTCATGCT GCCCAGAGCA 
AAACGAGGAC GAAGTGCAGA AAGCCTACAC ATGACCAGTG AACTGCAGCC CTCTCTTGAC 
AGGGCTAGGA GTGCTAGTAC CAACTGCTTG AGACCAGATA CTAGTTTGCA TTCACCAGAA 
CGAGAAAGGG GTAGATGGTC CCCCTCCCTA GATAGGAGGC GACCTGCTAG CCCCAGGATT 
CAAATCCAGC ATGCATCTCC GGAGAATGAC AGGCACTCCA GAAAGTCTGA AAGATGTAGC 
ATCCAAAAAC AGTCTAGGAA AGGCACAGCC TCTGATGCAG ACAGGGTTCT CCCACCATGC 
CTTTCTAGAA GGGGATACGC AACCCCAAGA GCAACCGATC AACCGGTCGT TAGGGGAAAG 
CATCCCACTC GTTCACGGTC GAGCGAGCAC TCTAGTGTCA GAACCCTGTG TTCTATGCAC 
CACCTTGCCC CCGGAGGGTC GGCGCCACCT TCTCCACTTC TGACAAGAAC GCACCGACAA 
GGAAGCCCAA CCCAGTCTCC TCCAGCAGAC ACATCCTTCG GCAGTCGCCG TGGAAGACAG 
CTCCCACAGG TGCCAGTTCG AAGCGGCAGT ATAGAACAAG CAAGCTTAGT AGTGGAGGAG 
CGAACGAGAC AGATGAAAAT GAAAGTTCAC CGATTTAAGC AGACAACAGG GTCTGGGTCT 
AGTCAAGAAC TTGACCACGA GCAATACTCC AAGTACAACA TACATAAAGA TCAGTACAGA 
AGCTGTGATA ACGCGTCTGC CAAGTCTTCA GATAGTGATG TCAGTGATGT GTCCGCCATT 
TCCAGAGCCA GCAGTACCTC ACGCCTCAGC AGCACAAGCT TTATGTCAGA GCAGTCTGAG 
CGCCCCAGGG GTAGGATCAG TTCATTTACC CCCAAAATGC AAGGCAGACG GATGGGGACT 
TCAGGAAGAG CCATCATCAA GAGCACCAGT GTAAGTGGAG AGATATATAC ACTGGAACGT 
AATGACGGTA GCCAGTCGGA CACGGCCGTG GGTACCGTCG GAGCCGGTGG AAAGAAACGA 
AGATCCAGCC TGAGCGCCAA AGTGGTAGCC ATTGTGTCTC GAAGAAGCAG GAGCACGTCA 
CAGCTCAGCC AGACAGAGTC GGGCCACAAG AAGTTGAAAA GCACCATCCA GAGGAGTACG 
GAAACAGGAA TGGCAGCTGA AATGCGGAAG ATGGTGAGAC AGCCGAGCCG GGAGTCCACG 
GATGGCAGCA TCAACAGTTA TAGCTCGGAA GGAAACTTGA TATTTCCTGG AGTTCGAGTA 
GGACCCGACA GTCAGTTCAG TGATTTCCTT GATGGGTTGG GACCAGCGCA GCTCGTTGGC 
CGTCAGACGC TCGCCACCCC GGCCATGGGC GATATCCAAA TCGGGATGGA GGATAAGAAG 
GGTCAGTTGG AGGTTGAGGT TATCAGAGCC CGGAGCCTTA CACAAAAACC TGGTTCCAAA 
TCTACACCCG CTCCCTATGT GAAAGTATAT CTTTTGGAAA ATGGAGCCTG TATTGCCAAA 
AAGAAGACAA GAATTGCACG GAAAACTCTC GATCCTTTGT ATCAGCAGTC CCTGGTTTTT 
GATGAAAGTC CACAGGGTAA AGTTCTTCAG GTGATTGTCT GGGGTGACTA TGGAAGAATG 
GACCACAAAT GCTTTATGGG TGTGGCTCAA ATCTTGTTGG AAGAACTTGA TCTATCCAGC 
ATGGTGATTG GATGGTATAA ATTGTTCCCT CCGTCCTCAC TGGTGGATCC CACTCTCGCT 
CCCCTGACCC GCCGGGCTTC CCAATCATCT CTGGAAAGTT CGTCCGGGCC TCCCTGCATC 
CGGTCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rims1 ( XM_017596658.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017452147.1 Rattus norvegicus regulating synaptic membrane exocytosis 1 (Rims1), transcript variant X4, mRNA.

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CloneID	ORa62875
Clone ID Related Accession (Same CDS sequence)	XM_017596658.1
Accession Version	XM_017596658.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4881bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	regulating synaptic membrane exocytosis protein 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005108.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCTCGG CCGTGGGGCC CCGAGGTCCT CGCCCACCCA CGGTGCCTCC CCCTATGCAA 
GAACTGCCCG ACCTGAGCCA CCTGACCGAG GAGGAGAGGA ACATTATCAT GGCAGTGATG 
GACCGGCAGA AGGAAGAGGA GGAAAAAGAA GAGGCCATGC TCAAGTGTGT TGTCAGGGAC 
ATGGCGAAGC CTGCTGCCTG CAAAACACCA AGAAATGCTG AAAGCCAGCC CCATCAACCA 
CCACTGAGAT TGCATCAACA GTTTGAAAGC TACAAGGAGC AAGTGAGAAA AATCGGAGAG 
GAAGCGAGGC GTTACCAGGG CGAGCACAAG GATGATGCCC CGACGTGTGG AATCTGTCAT 
AAGACAAAGT TTGCTGATGG ATGTGGCCAT CTCTGCTCCT ATTGTCGCAC CAAGTTCTGT 
GCACGCTGCG GAGGCCGTGT GTCTCTGCGA TCGAACAATG AGGACAAAGT GGTTATGTGG 
GTATGCAATT TATGTCGAAA GCAACAAGAA ATCTTAACGA AATCTGGAGC GTGGTTCTTT 
GGAAGTGGCC CTCAGCAGCC TAGTCAAGAT GGGACTCTGA GTGACACGGC CACAGGTGCT 
GGATCTGAGG TGCCAAGAGA AAAGAAAGCA AGGCTCCAAG AGCGATCAAG GTCTCAGACG 
CCCTTGAGTA CAGCAGCTGT CTCTTCCCAA GACACTGCTA CCCCCGGTGC ACCGTTGGAC 
AGGAACAAAG GGGCTGAGCC CTCACAGCAA GCCTTGGGTC CTGAACAGAA GCAGGCATCA 
AGATCAAGAA GCGAGCCACC GAGGGAAAGG AAGAAGGCTC CAGGGCTTTC AGAGCAGAAT 
GGCAAGGGAG GCCAGAAGAG CGAGCGCAAA CGTGTCCCCA AGTCTGTGGT GCAACCCGGG 
GAAGGGATCG CGGATGAGAG GGAGAGGAAA GAGAGGCGGG AAACCCGCAG GTTGGAGAAA 
GGGCGCTCCC AGGACTACTC AGACCGGCCT GAGAAACGCG ACAATGGCAG GGTGGCGGAA 
GACCAGAAGC AGAGGAAGGA GGAGGAGTAC CAGACTAGGT ACCGCAGCGA CCCTAACCTG 
GCTCGCTACC CGGTGAAGGC GCCGCCAGAG GAGCAGCAGA TGCGCATGCA CGCCCGGGTG 
TCCCGAGCGA GGCACGAGCG GCGCCACAGC GACGTGGCGC TCCCGCACAC CGAGGCAGCT 
GCCGCCGCGC CGGCTGAGGC CACGGCGGGC AAGCGCGCGC CGGCCACCGC CAGGGTCTCT 
CCCCCGGAGT CCCCGCGCGC ACGCGCGCCC GCCGCCCAGC CTCCCACCGA GCACGGGCCA 
CCGCCGCCGC GGCCAGTCCC GGGTCCCGCA GAGCCACCCG AGCCGCGCGT CCCCGAGCCG 
CTCCGTAAGC AGGGCCGCCT GGACCCGGCC TCGGCCGTGC TTCTGCGCAA GGCCAAGCGC 
GAGAAGGCGG AGAGCATGCT GCGGAACGAC TCGCTGAGCT CCGATCAGTC CGAGTCCGTG 
CGGCCATCCC CGCCCAAGCC TCACCGGCCC AAGCGGGGAG GCAAGAGACG TCAGATGTCG 
GTGAGCAGCT CGGAGGAGGA GGGCGTGTCC ACACCGGAGT ACACGAGCTG CGAGGACGTG 
GAGCTGGAGA GCGAGAGCGT GAGCGAGAAA GGTGACTTGG ATTACTACTG GTTGGATCCC 
GCCACGTGGC ACAGCAGGGA AACGTCGCCT ATCAGTTCGC ATCCTGTAAC GTGGCAGCCG 
TCTAAAGAGG GAGATCGACT AATCGGCCGT GTTATTCTTA ACAAAAGAAC AACCATGCCC 
AAAGAATCAG GTGCATTATT GGGTCTGAAG GTGGTTGGAG GAAAAATGAC GGACTTAGGG 
CGCCTTGGTG CTTTCATCAC CAAAGTAAAG AAGGGCAGCC TGGCAGACGT CGTCGGACAC 
CTAAGAGCAG GGGACGAAGT CCTAGAGTGG AATGGTAAAC CCCTGCCGGG AGCAACAAAC 
GAAGAAGTTT ACAACATTAT CTTAGAATCA AAATCAGAAC CTCAAGTTGA GATTATTGTT 
TCAAGGCCTA TTGGTGACAT CCCCAGGATC CCTGAGAGTT CCCATCCTCC CCTGGAGTCC 
AGTTCAAGTT CCTTTGAATC TCAGAAAATG GAAAGGCCTT CTATTTCTGT TATTTCTCCA 
ACCAGCCCTG GAGCTCTGAA AGATGCCCCA CAAGTCTTAC CAGGGCAACT CTCAGTGAAG 
CTATGGTATG ATAAAGTGGG GCACCAGCTG ATTGTAAATG TTCTACAAGC AACAGATCTA 
CCCCCTAGAG TAGATGGCCG TCCCAGGAAT CCCTATGTAA AAATGTATTT TCTTCCAGAT 
AGAAGCGACA AAAGTAAAAG GAGAACCAAA ACAGTAAAGA AACTTCTAGA GCCAAAATGG 
AACCAGACAT TTGTCTACTC ACACGTACAT CGTAGAGATT TTCGAGAGCG AATGTTAGAG 
ATTACCGTGT GGGACCAGCC GAGAGTACAG GACGAAGAGA GTGAATTTCT TGGAGAGATC 
CTCATAGAGT TGGAAACAGC GCTTTTAGAT GATGAGCCCC ATTGGTATAA ACTCCAGACA 
CATGACGAAT CTTCACTACC TCTGCCTCAG CCATCACCGT TCATGCCCAG GCGGCATATT 
CATGGAGAGA GCTCCAGCAA AAAGCTACAA AGATCTCAGC GAATCAGTGA TAGTGACATC 
TCAGATTATG AGGTTGATGA TGGTATTGGA GTAGTGCCTC CAGTGGGTTA TAGAGCTAGT 
GCTAGAGAGA GTAAAGCAAC CACGTTAACA GTGCCAGAGC AACAAAGAAC TACACATCAC 
CGCTCACGTT CCGTGTCTCC TCATCGCGGC GATGATCAGG GAAGGCCTCG TTCACGTTTA 
CCAAATGTGC CATTACAGAG GAGCTTAGAT GAAATTCATC CAACACGAAG GTCACGTTCT 
CCAACCCGAC ACCATGATGC CTCCCGAAGC CCGGCCGATC ACAGATCCAG ACATGTGGAA 
AGTCAATATT CGTCAGAGCC AGACAGTGAG CTTCTCATGC TGCCCAGAGC AAAACGAGGA 
CGAAGTGCAG AAAGCCTACA CATGACCAGT GAACTGCAGC CCTCTCTTGA CAGGGCTAGG 
AGTGCTAGTA CCAACTGCTT GAGACCAGAT ACTAGTTTGC ATTCACCAGA ACGAGAAAGG 
GGTAGATGGT CCCCCTCCCT AGATAGGAGG CGACCTGCTA GCCCCAGGAT TCAAATCCAG 
CATGCATCTC CGGAGAATGA CAGGCACTCC AGAAAGTCTG AAAGATGTAG CATCCAAAAA 
CAGTCTAGGA AAGGCACAGC CTCTGATGCA GACAGGGTTC TCCCACCATG CCTTTCTAGA 
AGGGGATACG CAACCCCAAG AGCAACCGAT CAACCGGTCG TTAGGGGAAA GCATCCCACT 
CGTTCACGGT CGAGCGAGCA CTCTAGTGTC AGAACCCTGT GTTCTATGCA CCACCTTGCC 
CCCGGAGGGT CGGCGCCACC TTCTCCACTT CTGACAAGAA CGCACCGACA AGGAAGCCCA 
ACCCAGTCTC CTCCAGCAGA CACATCCTTC GGCAGTCGCC GTGGAAGACA GCTCCCACAG 
GTGCCAGTTC GAAGCGGCAG TATAGAACAA GCAAGCTTAG TAGTGGAGGA GCGAACGAGA 
CAGATGAAAA TGAAAGTTCA CCGATTTAAG CAGACAACAG GGTCTGGGTC TAGTCAAGAA 
CTTGACCACG AGCAATACTC CAAGAAGTCA CGTTACATTA TGGAGTACAA CATACATAAA 
GATCAGTACA GAAGCTGTGA TAACGCGTCT GCCAAGTCTT CAGATAGTGA TGTCAGTGAT 
GTGTCCGCCA TTTCCAGAGC CAGCAGTACC TCACGCCTCA GCAGCACAAG CTTTATGTCA 
GAGCAGTCTG AGCGCCCCAG GGGTAGGATC AGTTCATTTA CCCCCAAAAT GCAAGGCAGA 
CGGATGGGGA CTTCAGGAAG AGCCATCATC AAGAGCACCA GTGTAAGTGG AGAGATATAT 
ACACTGGAAC GTAATGACGG TAGCCAGTCG GACACGGCCG TGGGTACCGT CGGAGCCGGT 
GGAAAGAAAC GAAGATCCAG CCTGAGCGCC AAAGTGGTAG CCATTGTGTC TCGAAGAAGC 
AGGAGCACGT CACAGCTCAG CCAGACAGAG TCGGGCCACA AGAAGTTGAA AAGCACCATC 
CAGAGGAGTA CGGAAACAGG AATGGCAGCT GAAATGCGGA AGATGGTGAG ACAGCCGAGC 
CGGGAGTCCA CGGATGGCAG CATCAACAGT TATAGCTCGG AAGGAAACTT GATATTTCCT 
GGAGTTCGAG TAGGACCCGA CAGTCAGTTC AGTGATTTCC TTGATGGGTT GGGACCAGCG 
CAGCTCGTTG GCCGTCAGAC GCTCGCCACC CCGGCCATGG GCGATATCCA AATCGGGATG 
GAGGATAAGA AGGGTCAGTT GGAGGTTGAG GTTATCAGAG CCCGGAGCCT TACACAAAAA 
CCTGGTTCCA AATCTACACC CGCTCCCTAT GTGAAAGTAT ATCTTTTGGA AAATGGAGCC 
TGTATTGCCA AAAAGAAGAC AAGAATTGCA CGGAAAACTC TCGATCCTTT GTATCAGCAG 
TCCCTGGTTT TTGATGAAAG TCCACAGGGT AAAGTTCTTC AGGTGATTGT CTGGGGTGAC 
TATGGAAGAA TGGACCACAA ATGCTTTATG GGTGTGGCTC AAATCTTGTT GGAAGAACTT 
GATCTATCCA GCATGGTGAT TGGATGGTAT AAATTGTTCC CTCCGTCCTC ACTGGTGGAT 
CCCACTCTCG CTCCCCTGAC CCGCCGGGCT TCCCAATCAT CTCTGGAAAG TTCGTCCGGG 
CCTCCCTGCA TCCGGTCATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017452147.1
CDS	315..5195
Translation	MSSAVGPRGPRPPTVPPPMQELPDLSHLTEEERNIIMAVMDRQKEEEEKEEAMLKCVVRDMAKPAACKTPRNAESQPHQPPLRLHQQFESYKEQVRKIGEEARRYQGEHKDDAPTCGICHKTKFADGCGHLCSYCRTKFCARCGGRVSLRSNNEDKVVMWVCNLCRKQQEILTKSGAWFFGSGPQQPSQDGTLSDTATGAGSEVPREKKARLQERSRSQTPLSTAAVSSQDTATPGAPLDRNKGAEPSQQALGPEQKQASRSRSEPPRERKKAPGLSEQNGKGGQKSERKRVPKSVVQPGEGIADERERKERRETRRLEKGRSQDYSDRPEKRDNGRVAEDQKQRKEEEYQTRYRSDPNLARYPVKAPPEEQQMRMHARVSRARHERRHSDVALPHTEAAAAAPAEATAGKRAPATARVSPPESPRARAPAAQPPTEHGPPPPRPVPGPAEPPEPRVPEPLRKQGRLDPASAVLLRKAKREKAESMLRNDSLSSDQSESVRPSPPKPHRPKRGGKRRQMSVSSSEEEGVSTPEYTSCEDVELESESVSEKGDLDYYWLDPATWHSRETSPISSHPVTWQPSKEGDRLIGRVILNKRTTMPKESGALLGLKVVGGKMTDLGRLGAFITKVKKGSLADVVGHLRAGDEVLEWNGKPLPGATNEEVYNIILESKSEPQVEIIVSRPIGDIPRIPESSHPPLESSSSSFESQKMERPSISVISPTSPGALKDAPQVLPGQLSVKLWYDKVGHQLIVNVLQATDLPPRVDGRPRNPYVKMYFLPDRSDKSKRRTKTVKKLLEPKWNQTFVYSHVHRRDFRERMLEITVWDQPRVQDEESEFLGEILIELETALLDDEPHWYKLQTHDESSLPLPQPSPFMPRRHIHGESSSKKLQRSQRISDSDISDYEVDDGIGVVPPVGYRASARESKATTLTVPEQQRTTHHRSRSVSPHRGDDQGRPRSRLPNVPLQRSLDEIHPTRRSRSPTRHHDASRSPADHRSRHVESQYSSEPDSELLMLPRAKRGRSAESLHMTSELQPSLDRARSASTNCLRPDTSLHSPERERGRWSPSLDRRRPASPRIQIQHASPENDRHSRKSERCSIQKQSRKGTASDADRVLPPCLSRRGYATPRATDQPVVRGKHPTRSRSSEHSSVRTLCSMHHLAPGGSAPPSPLLTRTHRQGSPTQSPPADTSFGSRRGRQLPQVPVRSGSIEQASLVVEERTRQMKMKVHRFKQTTGSGSSQELDHEQYSKKSRYIMEYNIHKDQYRSCDNASAKSSDSDVSDVSAISRASSTSRLSSTSFMSEQSERPRGRISSFTPKMQGRRMGTSGRAIIKSTSVSGEIYTLERNDGSQSDTAVGTVGAGGKKRRSSLSAKVVAIVSRRSRSTSQLSQTESGHKKLKSTIQRSTETGMAAEMRKMVRQPSRESTDGSINSYSSEGNLIFPGVRVGPDSQFSDFLDGLGPAQLVGRQTLATPAMGDIQIGMEDKKGQLEVEVIRARSLTQKPGSKSTPAPYVKVYLLENGACIAKKKTRIARKTLDPLYQQSLVFDESPQGKVLQVIVWGDYGRMDHKCFMGVAQILLEELDLSSMVIGWYKLFPPSSLVDPTLAPLTRRASQSSLESSSGPPCIRS

Target ORF information:

RefSeq Version	XM_017596658.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus regulating synaptic membrane exocytosis 1 (Rims1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017596658.1

ORF Insert Sequence:

ATGTCCTCGG CCGTGGGGCC CCGAGGTCCT CGCCCACCCA CGGTGCCTCC CCCTATGCAA 
GAACTGCCCG ACCTGAGCCA CCTGACCGAG GAGGAGAGGA ACATTATCAT GGCAGTGATG 
GACCGGCAGA AGGAAGAGGA GGAAAAAGAA GAGGCCATGC TCAAGTGTGT TGTCAGGGAC 
ATGGCGAAGC CTGCTGCCTG CAAAACACCA AGAAATGCTG AAAGCCAGCC CCATCAACCA 
CCACTGAGAT TGCATCAACA GTTTGAAAGC TACAAGGAGC AAGTGAGAAA AATCGGAGAG 
GAAGCGAGGC GTTACCAGGG CGAGCACAAG GATGATGCCC CGACGTGTGG AATCTGTCAT 
AAGACAAAGT TTGCTGATGG ATGTGGCCAT CTCTGCTCCT ATTGTCGCAC CAAGTTCTGT 
GCACGCTGCG GAGGCCGTGT GTCTCTGCGA TCGAACAATG AGGACAAAGT GGTTATGTGG 
GTATGCAATT TATGTCGAAA GCAACAAGAA ATCTTAACGA AATCTGGAGC GTGGTTCTTT 
GGAAGTGGCC CTCAGCAGCC TAGTCAAGAT GGGACTCTGA GTGACACGGC CACAGGTGCT 
GGATCTGAGG TGCCAAGAGA AAAGAAAGCA AGGCTCCAAG AGCGATCAAG GTCTCAGACG 
CCCTTGAGTA CAGCAGCTGT CTCTTCCCAA GACACTGCTA CCCCCGGTGC ACCGTTGGAC 
AGGAACAAAG GGGCTGAGCC CTCACAGCAA GCCTTGGGTC CTGAACAGAA GCAGGCATCA 
AGATCAAGAA GCGAGCCACC GAGGGAAAGG AAGAAGGCTC CAGGGCTTTC AGAGCAGAAT 
GGCAAGGGAG GCCAGAAGAG CGAGCGCAAA CGTGTCCCCA AGTCTGTGGT GCAACCCGGG 
GAAGGGATCG CGGATGAGAG GGAGAGGAAA GAGAGGCGGG AAACCCGCAG GTTGGAGAAA 
GGGCGCTCCC AGGACTACTC AGACCGGCCT GAGAAACGCG ACAATGGCAG GGTGGCGGAA 
GACCAGAAGC AGAGGAAGGA GGAGGAGTAC CAGACTAGGT ACCGCAGCGA CCCTAACCTG 
GCTCGCTACC CGGTGAAGGC GCCGCCAGAG GAGCAGCAGA TGCGCATGCA CGCCCGGGTG 
TCCCGAGCGA GGCACGAGCG GCGCCACAGC GACGTGGCGC TCCCGCACAC CGAGGCAGCT 
GCCGCCGCGC CGGCTGAGGC CACGGCGGGC AAGCGCGCGC CGGCCACCGC CAGGGTCTCT 
CCCCCGGAGT CCCCGCGCGC ACGCGCGCCC GCCGCCCAGC CTCCCACCGA GCACGGGCCA 
CCGCCGCCGC GGCCAGTCCC GGGTCCCGCA GAGCCACCCG AGCCGCGCGT CCCCGAGCCG 
CTCCGTAAGC AGGGCCGCCT GGACCCGGCC TCGGCCGTGC TTCTGCGCAA GGCCAAGCGC 
GAGAAGGCGG AGAGCATGCT GCGGAACGAC TCGCTGAGCT CCGATCAGTC CGAGTCCGTG 
CGGCCATCCC CGCCCAAGCC TCACCGGCCC AAGCGGGGAG GCAAGAGACG TCAGATGTCG 
GTGAGCAGCT CGGAGGAGGA GGGCGTGTCC ACACCGGAGT ACACGAGCTG CGAGGACGTG 
GAGCTGGAGA GCGAGAGCGT GAGCGAGAAA GGTGACTTGG ATTACTACTG GTTGGATCCC 
GCCACGTGGC ACAGCAGGGA AACGTCGCCT ATCAGTTCGC ATCCTGTAAC GTGGCAGCCG 
TCTAAAGAGG GAGATCGACT AATCGGCCGT GTTATTCTTA ACAAAAGAAC AACCATGCCC 
AAAGAATCAG GTGCATTATT GGGTCTGAAG GTGGTTGGAG GAAAAATGAC GGACTTAGGG 
CGCCTTGGTG CTTTCATCAC CAAAGTAAAG AAGGGCAGCC TGGCAGACGT CGTCGGACAC 
CTAAGAGCAG GGGACGAAGT CCTAGAGTGG AATGGTAAAC CCCTGCCGGG AGCAACAAAC 
GAAGAAGTTT ACAACATTAT CTTAGAATCA AAATCAGAAC CTCAAGTTGA GATTATTGTT 
TCAAGGCCTA TTGGTGACAT CCCCAGGATC CCTGAGAGTT CCCATCCTCC CCTGGAGTCC 
AGTTCAAGTT CCTTTGAATC TCAGAAAATG GAAAGGCCTT CTATTTCTGT TATTTCTCCA 
ACCAGCCCTG GAGCTCTGAA AGATGCCCCA CAAGTCTTAC CAGGGCAACT CTCAGTGAAG 
CTATGGTATG ATAAAGTGGG GCACCAGCTG ATTGTAAATG TTCTACAAGC AACAGATCTA 
CCCCCTAGAG TAGATGGCCG TCCCAGGAAT CCCTATGTAA AAATGTATTT TCTTCCAGAT 
AGAAGCGACA AAAGTAAAAG GAGAACCAAA ACAGTAAAGA AACTTCTAGA GCCAAAATGG 
AACCAGACAT TTGTCTACTC ACACGTACAT CGTAGAGATT TTCGAGAGCG AATGTTAGAG 
ATTACCGTGT GGGACCAGCC GAGAGTACAG GACGAAGAGA GTGAATTTCT TGGAGAGATC 
CTCATAGAGT TGGAAACAGC GCTTTTAGAT GATGAGCCCC ATTGGTATAA ACTCCAGACA 
CATGACGAAT CTTCACTACC TCTGCCTCAG CCATCACCGT TCATGCCCAG GCGGCATATT 
CATGGAGAGA GCTCCAGCAA AAAGCTACAA AGATCTCAGC GAATCAGTGA TAGTGACATC 
TCAGATTATG AGGTTGATGA TGGTATTGGA GTAGTGCCTC CAGTGGGTTA TAGAGCTAGT 
GCTAGAGAGA GTAAAGCAAC CACGTTAACA GTGCCAGAGC AACAAAGAAC TACACATCAC 
CGCTCACGTT CCGTGTCTCC TCATCGCGGC GATGATCAGG GAAGGCCTCG TTCACGTTTA 
CCAAATGTGC CATTACAGAG GAGCTTAGAT GAAATTCATC CAACACGAAG GTCACGTTCT 
CCAACCCGAC ACCATGATGC CTCCCGAAGC CCGGCCGATC ACAGATCCAG ACATGTGGAA 
AGTCAATATT CGTCAGAGCC AGACAGTGAG CTTCTCATGC TGCCCAGAGC AAAACGAGGA 
CGAAGTGCAG AAAGCCTACA CATGACCAGT GAACTGCAGC CCTCTCTTGA CAGGGCTAGG 
AGTGCTAGTA CCAACTGCTT GAGACCAGAT ACTAGTTTGC ATTCACCAGA ACGAGAAAGG 
GGTAGATGGT CCCCCTCCCT AGATAGGAGG CGACCTGCTA GCCCCAGGAT TCAAATCCAG 
CATGCATCTC CGGAGAATGA CAGGCACTCC AGAAAGTCTG AAAGATGTAG CATCCAAAAA 
CAGTCTAGGA AAGGCACAGC CTCTGATGCA GACAGGGTTC TCCCACCATG CCTTTCTAGA 
AGGGGATACG CAACCCCAAG AGCAACCGAT CAACCGGTCG TTAGGGGAAA GCATCCCACT 
CGTTCACGGT CGAGCGAGCA CTCTAGTGTC AGAACCCTGT GTTCTATGCA CCACCTTGCC 
CCCGGAGGGT CGGCGCCACC TTCTCCACTT CTGACAAGAA CGCACCGACA AGGAAGCCCA 
ACCCAGTCTC CTCCAGCAGA CACATCCTTC GGCAGTCGCC GTGGAAGACA GCTCCCACAG 
GTGCCAGTTC GAAGCGGCAG TATAGAACAA GCAAGCTTAG TAGTGGAGGA GCGAACGAGA 
CAGATGAAAA TGAAAGTTCA CCGATTTAAG CAGACAACAG GGTCTGGGTC TAGTCAAGAA 
CTTGACCACG AGCAATACTC CAAGAAGTCA CGTTACATTA TGGAGTACAA CATACATAAA 
GATCAGTACA GAAGCTGTGA TAACGCGTCT GCCAAGTCTT CAGATAGTGA TGTCAGTGAT 
GTGTCCGCCA TTTCCAGAGC CAGCAGTACC TCACGCCTCA GCAGCACAAG CTTTATGTCA 
GAGCAGTCTG AGCGCCCCAG GGGTAGGATC AGTTCATTTA CCCCCAAAAT GCAAGGCAGA 
CGGATGGGGA CTTCAGGAAG AGCCATCATC AAGAGCACCA GTGTAAGTGG AGAGATATAT 
ACACTGGAAC GTAATGACGG TAGCCAGTCG GACACGGCCG TGGGTACCGT CGGAGCCGGT 
GGAAAGAAAC GAAGATCCAG CCTGAGCGCC AAAGTGGTAG CCATTGTGTC TCGAAGAAGC 
AGGAGCACGT CACAGCTCAG CCAGACAGAG TCGGGCCACA AGAAGTTGAA AAGCACCATC 
CAGAGGAGTA CGGAAACAGG AATGGCAGCT GAAATGCGGA AGATGGTGAG ACAGCCGAGC 
CGGGAGTCCA CGGATGGCAG CATCAACAGT TATAGCTCGG AAGGAAACTT GATATTTCCT 
GGAGTTCGAG TAGGACCCGA CAGTCAGTTC AGTGATTTCC TTGATGGGTT GGGACCAGCG 
CAGCTCGTTG GCCGTCAGAC GCTCGCCACC CCGGCCATGG GCGATATCCA AATCGGGATG 
GAGGATAAGA AGGGTCAGTT GGAGGTTGAG GTTATCAGAG CCCGGAGCCT TACACAAAAA 
CCTGGTTCCA AATCTACACC CGCTCCCTAT GTGAAAGTAT ATCTTTTGGA AAATGGAGCC 
TGTATTGCCA AAAAGAAGAC AAGAATTGCA CGGAAAACTC TCGATCCTTT GTATCAGCAG 
TCCCTGGTTT TTGATGAAAG TCCACAGGGT AAAGTTCTTC AGGTGATTGT CTGGGGTGAC 
TATGGAAGAA TGGACCACAA ATGCTTTATG GGTGTGGCTC AAATCTTGTT GGAAGAACTT 
GATCTATCCA GCATGGTGAT TGGATGGTAT AAATTGTTCC CTCCGTCCTC ACTGGTGGAT 
CCCACTCTCG CTCCCCTGAC CCGCCGGGCT TCCCAATCAT CTCTGGAAAG TTCGTCCGGG 
CCTCCCTGCA TCCGGTCATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rims1 ( XM_017596659.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017452148.1 Rattus norvegicus regulating synaptic membrane exocytosis 1 (Rims1), transcript variant X5, mRNA.

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CloneID	ORa62876
Clone ID Related Accession (Same CDS sequence)	XM_017596659.1
Accession Version	XM_017596659.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4869bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	regulating synaptic membrane exocytosis protein 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005108.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCTCGG CCGTGGGGCC CCGAGGTCCT CGCCCACCCA CGGTGCCTCC CCCTATGCAA 
GAACTGCCCG ACCTGAGCCA CCTGACCGAG GAGGAGAGGA ACATTATCAT GGCAGTGATG 
GACCGGCAGA AGGAAGAGGA GGAAAAAGAA GAGGCCATGC TCAAGAACAT TTTCAGATGT 
GTCTGTGTTC CCAGAAAGCC AAGCAGCGAA GAGGGAGGCC CAGAAAGAGA CTGGAGATTG 
CATCAACAGT TTGAAAGCTA CAAGGAGCAA GTGAGAAAAA TCGGAGAGGA AGCGAGGCGT 
TACCAGGGCG AGCACAAGGA TGATGCCCCG ACGTGTGGAA TCTGTCATAA GACAAAGTTT 
GCTGATGGAT GTGGCCATCT CTGCTCCTAT TGTCGCACCA AGTTCTGTGC ACGCTGCGGA 
GGCCGTGTGT CTCTGCGATC GAACAATGAG GACAAAGTGG TTATGTGGGT ATGCAATTTA 
TGTCGAAAGC AACAAGAAAT CTTAACGAAA TCTGGAGCGT GGTTCTTTGG AAGTGGCCCT 
CAGCAGCCTA GTCAAGATGG GACTCTGAGT GACACGGCCA CAGGTGCTGG ATCTGAGGTG 
CCAAGAGAAA AGAAAGCAAG GCTCCAAGAG CGATCAAGGT CTCAGACGCC CTTGAGTACA 
GCAGCTGTCT CTTCCCAAGA CACTGCTACC CCCGGTGCAC CGTTGGACAG GAACAAAGGG 
GCTGAGCCCT CACAGCAAGC CTTGGGTCCT GAACAGAAGC AGGCATCAAG ATCAAGAAGC 
GAGCCACCGA GGGAAAGGAA GAAGGCTCCA GGGCTTTCAG AGCAGAATGG CAAGGGAGGC 
CAGAAGAGCG AGCGCAAACG TGTCCCCAAG TCTGTGGTGC AACCCGGGGA AGGGATCGCG 
GATGAGAGGG AGAGGAAAGA GAGGCGGGAA ACCCGCAGGT TGGAGAAAGG GCGCTCCCAG 
GACTACTCAG ACCGGCCTGA GAAACGCGAC AATGGCAGGG TGGCGGAAGA CCAGAAGCAG 
AGGAAGGAGG AGGAGTACCA GACTAGGTAC CGCAGCGACC CTAACCTGGC TCGCTACCCG 
GTGAAGGCGC CGCCAGAGGA GCAGCAGATG CGCATGCACG CCCGGGTGTC CCGAGCGAGG 
CACGAGCGGC GCCACAGCGA CGTGGCGCTC CCGCACACCG AGGCAGCTGC CGCCGCGCCG 
GCTGAGGCCA CGGCGGGCAA GCGCGCGCCG GCCACCGCCA GGGTCTCTCC CCCGGAGTCC 
CCGCGCGCAC GCGCGCCCGC CGCCCAGCCT CCCACCGAGC ACGGGCCACC GCCGCCGCGG 
CCAGTCCCGG GTCCCGCAGA GCCACCCGAG CCGCGCGTCC CCGAGCCGCT CCGTAAGCAG 
GGCCGCCTGG ACCCGGCCTC GGCCGTGCTT CTGCGCAAGG CCAAGCGCGA GAAGGCGGAG 
AGCATGCTGC GGAACGACTC GCTGAGCTCC GATCAGTCCG AGTCCGTGCG GCCATCCCCG 
CCCAAGCCTC ACCGGCCCAA GCGGGGAGGC AAGAGACGTC AGATGTCGGT GAGCAGCTCG 
GAGGAGGAGG GCGTGTCCAC ACCGGAGTAC ACGAGCTGCG AGGACGTGGA GCTGGAGAGC 
GAGAGCGTGA GCGAGAAAGG TGACTTGGAT TACTACTGGT TGGATCCCGC CACGTGGCAC 
AGCAGGGAAA CGTCGCCTAT CAGTTCGCAT CCTGTAACGT GGCAGCCGTC TAAAGAGGGA 
GATCGACTAA TCGGCCGTGT TATTCTTAAC AAAAGAACAA CCATGCCCAA AGAATCAGGT 
GCATTATTGG GTCTGAAGGT GGTTGGAGGA AAAATGACGG ACTTAGGGCG CCTTGGTGCT 
TTCATCACCA AAGTAAAGAA GGGCAGCCTG GCAGACGTCG TCGGACACCT AAGAGCAGGG 
GACGAAGTCC TAGAGTGGAA TGGTAAACCC CTGCCGGGAG CAACAAACGA AGAAGTTTAC 
AACATTATCT TAGAATCAAA ATCAGAACCT CAAGTTGAGA TTATTGTTTC AAGGCCTATT 
GGTGACATCC CCAGGATCCC TGAGAGTTCC CATCCTCCCC TGGAGTCCAG TTCAAGTTCC 
TTTGAATCTC AGAAAATGGA AAGGCCTTCT ATTTCTGTTA TTTCTCCAAC CAGCCCTGGA 
GCTCTGAAAG ATGCCCCACA AGTCTTACCA GGGCAACTCT CAGTGAAGCT ATGGTATGAT 
AAAGTGGGGC ACCAGCTGAT TGTAAATGTT CTACAAGCAA CAGATCTACC CCCTAGAGTA 
GATGGCCGTC CCAGGAATCC CTATGTAAAA ATGTATTTTC TTCCAGATAG AAGCGACAAA 
AGTAAAAGGA GAACCAAAAC AGTAAAGAAA CTTCTAGAGC CAAAATGGAA CCAGACATTT 
GTCTACTCAC ACGTACATCG TAGAGATTTT CGAGAGCGAA TGTTAGAGAT TACCGTGTGG 
GACCAGCCGA GAGTACAGGA CGAAGAGAGT GAATTTCTTG GAGAGATCCT CATAGAGTTG 
GAAACAGCGC TTTTAGATGA TGAGCCCCAT TGGTATAAAC TCCAGACACA TGACGAATCT 
TCACTACCTC TGCCTCAGCC ATCACCGTTC ATGCCCAGGC GGCATATTCA TGGAGAGAGC 
TCCAGCAAAA AGCTACAAAG ATCTCAGCGA ATCAGTGATA GTGACATCTC AGATTATGAG 
GTTGATGATG GTATTGGAGT AGTGCCTCCA GTGGGTTATA GAGCTAGTGC TAGAGAGAGT 
AAAGCAACCA CGTTAACAGT GCCAGAGCAA CAAAGAACTA CACATCACCG CTCACGTTCC 
GTGTCTCCTC ATCGCGGCGA TGATCAGGGA AGGCCTCGTT CACGTTTACC AAATGTGCCA 
TTACAGAGGA GCTTAGATGA AATTCATCCA ACACGAAGGT CACGTTCTCC AACCCGACAC 
CATGATGCCT CCCGAAGCCC GGCCGATCAC AGATCCAGAC ATGTGGAAAG TCAATATTCG 
TCAGAGCCAG ACAGTGAGCT TCTCATGCTG CCCAGAGCAA AACGAGGACG AAGTGCAGAA 
AGCCTACACA TGACCAGTGA ACTGCAGCCC TCTCTTGACA GGGCTAGGAG TGCTAGTACC 
AACTGCTTGA GACCAGATAC TAGTTTGCAT TCACCAGAAC GAGAAAGGGG TAGATGGTCC 
CCCTCCCTAG ATAGGAGGCG ACCTGCTAGC CCCAGGATTC AAATCCAGCA TGCATCTCCG 
GAGAATGACA GGCACTCCAG AAAGTCTGAA AGATGTAGCA TCCAAAAACA GTCTAGGAAA 
GGCACAGCCT CTGATGCAGA CAGGGTTCTC CCACCATGCC TTTCTAGAAG GGGATACGCA 
ACCCCAAGAG CAACCGATCA ACCGGTCGTT AGGGGAAAGC ATCCCACTCG TTCACGGTCG 
AGCGAGCACT CTAGTGTCAG AACCCTGTGT TCTATGCACC ACCTTGCCCC CGGAGGGTCG 
GCGCCACCTT CTCCACTTCT GACAAGAACG CACCGACAAG GAAGCCCAAC CCAGTCTCCT 
CCAGCAGACA CATCCTTCGG CAGTCGCCGT GGAAGACAGC TCCCACAGGT GCCAGTTCGA 
AGCGGCAGTA TAGAACAAGC AAGCTTAGTA GTGGAGGAGC GAACGAGACA GATGAAAATG 
AAAGTTCACC GATTTAAGCA GACAACAGGG TCTGGGTCTA GTCAAGAACT TGACCACGAG 
CAATACTCCA AGAAGTCACG TTACATTATG GAGTACAACA TACATAAAGA TCAGTACAGA 
AGCTGTGATA ACGCGTCTGC CAAGTCTTCA GATAGTGATG TCAGTGATGT GTCCGCCATT 
TCCAGAGCCA GCAGTACCTC ACGCCTCAGC AGCACAAGCT TTATGTCAGA GCAGTCTGAG 
CGCCCCAGGG GTAGGATCAG TTCATTTACC CCCAAAATGC AAGGCAGACG GATGGGGACT 
TCAGGAAGAG CCATCATCAA GAGCACCAGT GTAAGTGGAG AGATATATAC ACTGGAACGT 
AATGACGGTA GCCAGTCGGA CACGGCCGTG GGTACCGTCG GAGCCGGTGG AAAGAAACGA 
AGATCCAGCC TGAGCGCCAA AGTGGTAGCC ATTGTGTCTC GAAGAAGCAG GAGCACGTCA 
CAGCTCAGCC AGACAGAGTC GGGCCACAAG AAGTTGAAAA GCACCATCCA GAGGAGTACG 
GAAACAGGAA TGGCAGCTGA AATGCGGAAG ATGGTGAGAC AGCCGAGCCG GGAGTCCACG 
GATGGCAGCA TCAACAGTTA TAGCTCGGAA GGAAACTTGA TATTTCCTGG AGTTCGAGTA 
GGACCCGACA GTCAGTTCAG TGATTTCCTT GATGGGTTGG GACCAGCGCA GCTCGTTGGC 
CGTCAGACGC TCGCCACCCC GGCCATGGGC GATATCCAAA TCGGGATGGA GGATAAGAAG 
GGTCAGTTGG AGGTTGAGGT TATCAGAGCC CGGAGCCTTA CACAAAAACC TGGTTCCAAA 
TCTACACCCG CTCCCTATGT GAAAGTATAT CTTTTGGAAA ATGGAGCCTG TATTGCCAAA 
AAGAAGACAA GAATTGCACG GAAAACTCTC GATCCTTTGT ATCAGCAGTC CCTGGTTTTT 
GATGAAAGTC CACAGGGTAA AGTTCTTCAG GTGATTGTCT GGGGTGACTA TGGAAGAATG 
GACCACAAAT GCTTTATGGG TGTGGCTCAA ATCTTGTTGG AAGAACTTGA TCTATCCAGC 
ATGGTGATTG GATGGTATAA ATTGTTCCCT CCGTCCTCAC TGGTGGATCC CACTCTCGCT 
CCCCTGACCC GCCGGGCTTC CCAATCATCT CTGGAAAGTT CGTCCGGGCC TCCCTGCATC 
CGGTCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017452148.1
CDS	315..5183
Translation	MSSAVGPRGPRPPTVPPPMQELPDLSHLTEEERNIIMAVMDRQKEEEEKEEAMLKNIFRCVCVPRKPSSEEGGPERDWRLHQQFESYKEQVRKIGEEARRYQGEHKDDAPTCGICHKTKFADGCGHLCSYCRTKFCARCGGRVSLRSNNEDKVVMWVCNLCRKQQEILTKSGAWFFGSGPQQPSQDGTLSDTATGAGSEVPREKKARLQERSRSQTPLSTAAVSSQDTATPGAPLDRNKGAEPSQQALGPEQKQASRSRSEPPRERKKAPGLSEQNGKGGQKSERKRVPKSVVQPGEGIADERERKERRETRRLEKGRSQDYSDRPEKRDNGRVAEDQKQRKEEEYQTRYRSDPNLARYPVKAPPEEQQMRMHARVSRARHERRHSDVALPHTEAAAAAPAEATAGKRAPATARVSPPESPRARAPAAQPPTEHGPPPPRPVPGPAEPPEPRVPEPLRKQGRLDPASAVLLRKAKREKAESMLRNDSLSSDQSESVRPSPPKPHRPKRGGKRRQMSVSSSEEEGVSTPEYTSCEDVELESESVSEKGDLDYYWLDPATWHSRETSPISSHPVTWQPSKEGDRLIGRVILNKRTTMPKESGALLGLKVVGGKMTDLGRLGAFITKVKKGSLADVVGHLRAGDEVLEWNGKPLPGATNEEVYNIILESKSEPQVEIIVSRPIGDIPRIPESSHPPLESSSSSFESQKMERPSISVISPTSPGALKDAPQVLPGQLSVKLWYDKVGHQLIVNVLQATDLPPRVDGRPRNPYVKMYFLPDRSDKSKRRTKTVKKLLEPKWNQTFVYSHVHRRDFRERMLEITVWDQPRVQDEESEFLGEILIELETALLDDEPHWYKLQTHDESSLPLPQPSPFMPRRHIHGESSSKKLQRSQRISDSDISDYEVDDGIGVVPPVGYRASARESKATTLTVPEQQRTTHHRSRSVSPHRGDDQGRPRSRLPNVPLQRSLDEIHPTRRSRSPTRHHDASRSPADHRSRHVESQYSSEPDSELLMLPRAKRGRSAESLHMTSELQPSLDRARSASTNCLRPDTSLHSPERERGRWSPSLDRRRPASPRIQIQHASPENDRHSRKSERCSIQKQSRKGTASDADRVLPPCLSRRGYATPRATDQPVVRGKHPTRSRSSEHSSVRTLCSMHHLAPGGSAPPSPLLTRTHRQGSPTQSPPADTSFGSRRGRQLPQVPVRSGSIEQASLVVEERTRQMKMKVHRFKQTTGSGSSQELDHEQYSKKSRYIMEYNIHKDQYRSCDNASAKSSDSDVSDVSAISRASSTSRLSSTSFMSEQSERPRGRISSFTPKMQGRRMGTSGRAIIKSTSVSGEIYTLERNDGSQSDTAVGTVGAGGKKRRSSLSAKVVAIVSRRSRSTSQLSQTESGHKKLKSTIQRSTETGMAAEMRKMVRQPSRESTDGSINSYSSEGNLIFPGVRVGPDSQFSDFLDGLGPAQLVGRQTLATPAMGDIQIGMEDKKGQLEVEVIRARSLTQKPGSKSTPAPYVKVYLLENGACIAKKKTRIARKTLDPLYQQSLVFDESPQGKVLQVIVWGDYGRMDHKCFMGVAQILLEELDLSSMVIGWYKLFPPSSLVDPTLAPLTRRASQSSLESSSGPPCIRS

Target ORF information:

RefSeq Version	XM_017596659.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus regulating synaptic membrane exocytosis 1 (Rims1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017596659.1

ORF Insert Sequence:

ATGTCCTCGG CCGTGGGGCC CCGAGGTCCT CGCCCACCCA CGGTGCCTCC CCCTATGCAA 
GAACTGCCCG ACCTGAGCCA CCTGACCGAG GAGGAGAGGA ACATTATCAT GGCAGTGATG 
GACCGGCAGA AGGAAGAGGA GGAAAAAGAA GAGGCCATGC TCAAGAACAT TTTCAGATGT 
GTCTGTGTTC CCAGAAAGCC AAGCAGCGAA GAGGGAGGCC CAGAAAGAGA CTGGAGATTG 
CATCAACAGT TTGAAAGCTA CAAGGAGCAA GTGAGAAAAA TCGGAGAGGA AGCGAGGCGT 
TACCAGGGCG AGCACAAGGA TGATGCCCCG ACGTGTGGAA TCTGTCATAA GACAAAGTTT 
GCTGATGGAT GTGGCCATCT CTGCTCCTAT TGTCGCACCA AGTTCTGTGC ACGCTGCGGA 
GGCCGTGTGT CTCTGCGATC GAACAATGAG GACAAAGTGG TTATGTGGGT ATGCAATTTA 
TGTCGAAAGC AACAAGAAAT CTTAACGAAA TCTGGAGCGT GGTTCTTTGG AAGTGGCCCT 
CAGCAGCCTA GTCAAGATGG GACTCTGAGT GACACGGCCA CAGGTGCTGG ATCTGAGGTG 
CCAAGAGAAA AGAAAGCAAG GCTCCAAGAG CGATCAAGGT CTCAGACGCC CTTGAGTACA 
GCAGCTGTCT CTTCCCAAGA CACTGCTACC CCCGGTGCAC CGTTGGACAG GAACAAAGGG 
GCTGAGCCCT CACAGCAAGC CTTGGGTCCT GAACAGAAGC AGGCATCAAG ATCAAGAAGC 
GAGCCACCGA GGGAAAGGAA GAAGGCTCCA GGGCTTTCAG AGCAGAATGG CAAGGGAGGC 
CAGAAGAGCG AGCGCAAACG TGTCCCCAAG TCTGTGGTGC AACCCGGGGA AGGGATCGCG 
GATGAGAGGG AGAGGAAAGA GAGGCGGGAA ACCCGCAGGT TGGAGAAAGG GCGCTCCCAG 
GACTACTCAG ACCGGCCTGA GAAACGCGAC AATGGCAGGG TGGCGGAAGA CCAGAAGCAG 
AGGAAGGAGG AGGAGTACCA GACTAGGTAC CGCAGCGACC CTAACCTGGC TCGCTACCCG 
GTGAAGGCGC CGCCAGAGGA GCAGCAGATG CGCATGCACG CCCGGGTGTC CCGAGCGAGG 
CACGAGCGGC GCCACAGCGA CGTGGCGCTC CCGCACACCG AGGCAGCTGC CGCCGCGCCG 
GCTGAGGCCA CGGCGGGCAA GCGCGCGCCG GCCACCGCCA GGGTCTCTCC CCCGGAGTCC 
CCGCGCGCAC GCGCGCCCGC CGCCCAGCCT CCCACCGAGC ACGGGCCACC GCCGCCGCGG 
CCAGTCCCGG GTCCCGCAGA GCCACCCGAG CCGCGCGTCC CCGAGCCGCT CCGTAAGCAG 
GGCCGCCTGG ACCCGGCCTC GGCCGTGCTT CTGCGCAAGG CCAAGCGCGA GAAGGCGGAG 
AGCATGCTGC GGAACGACTC GCTGAGCTCC GATCAGTCCG AGTCCGTGCG GCCATCCCCG 
CCCAAGCCTC ACCGGCCCAA GCGGGGAGGC AAGAGACGTC AGATGTCGGT GAGCAGCTCG 
GAGGAGGAGG GCGTGTCCAC ACCGGAGTAC ACGAGCTGCG AGGACGTGGA GCTGGAGAGC 
GAGAGCGTGA GCGAGAAAGG TGACTTGGAT TACTACTGGT TGGATCCCGC CACGTGGCAC 
AGCAGGGAAA CGTCGCCTAT CAGTTCGCAT CCTGTAACGT GGCAGCCGTC TAAAGAGGGA 
GATCGACTAA TCGGCCGTGT TATTCTTAAC AAAAGAACAA CCATGCCCAA AGAATCAGGT 
GCATTATTGG GTCTGAAGGT GGTTGGAGGA AAAATGACGG ACTTAGGGCG CCTTGGTGCT 
TTCATCACCA AAGTAAAGAA GGGCAGCCTG GCAGACGTCG TCGGACACCT AAGAGCAGGG 
GACGAAGTCC TAGAGTGGAA TGGTAAACCC CTGCCGGGAG CAACAAACGA AGAAGTTTAC 
AACATTATCT TAGAATCAAA ATCAGAACCT CAAGTTGAGA TTATTGTTTC AAGGCCTATT 
GGTGACATCC CCAGGATCCC TGAGAGTTCC CATCCTCCCC TGGAGTCCAG TTCAAGTTCC 
TTTGAATCTC AGAAAATGGA AAGGCCTTCT ATTTCTGTTA TTTCTCCAAC CAGCCCTGGA 
GCTCTGAAAG ATGCCCCACA AGTCTTACCA GGGCAACTCT CAGTGAAGCT ATGGTATGAT 
AAAGTGGGGC ACCAGCTGAT TGTAAATGTT CTACAAGCAA CAGATCTACC CCCTAGAGTA 
GATGGCCGTC CCAGGAATCC CTATGTAAAA ATGTATTTTC TTCCAGATAG AAGCGACAAA 
AGTAAAAGGA GAACCAAAAC AGTAAAGAAA CTTCTAGAGC CAAAATGGAA CCAGACATTT 
GTCTACTCAC ACGTACATCG TAGAGATTTT CGAGAGCGAA TGTTAGAGAT TACCGTGTGG 
GACCAGCCGA GAGTACAGGA CGAAGAGAGT GAATTTCTTG GAGAGATCCT CATAGAGTTG 
GAAACAGCGC TTTTAGATGA TGAGCCCCAT TGGTATAAAC TCCAGACACA TGACGAATCT 
TCACTACCTC TGCCTCAGCC ATCACCGTTC ATGCCCAGGC GGCATATTCA TGGAGAGAGC 
TCCAGCAAAA AGCTACAAAG ATCTCAGCGA ATCAGTGATA GTGACATCTC AGATTATGAG 
GTTGATGATG GTATTGGAGT AGTGCCTCCA GTGGGTTATA GAGCTAGTGC TAGAGAGAGT 
AAAGCAACCA CGTTAACAGT GCCAGAGCAA CAAAGAACTA CACATCACCG CTCACGTTCC 
GTGTCTCCTC ATCGCGGCGA TGATCAGGGA AGGCCTCGTT CACGTTTACC AAATGTGCCA 
TTACAGAGGA GCTTAGATGA AATTCATCCA ACACGAAGGT CACGTTCTCC AACCCGACAC 
CATGATGCCT CCCGAAGCCC GGCCGATCAC AGATCCAGAC ATGTGGAAAG TCAATATTCG 
TCAGAGCCAG ACAGTGAGCT TCTCATGCTG CCCAGAGCAA AACGAGGACG AAGTGCAGAA 
AGCCTACACA TGACCAGTGA ACTGCAGCCC TCTCTTGACA GGGCTAGGAG TGCTAGTACC 
AACTGCTTGA GACCAGATAC TAGTTTGCAT TCACCAGAAC GAGAAAGGGG TAGATGGTCC 
CCCTCCCTAG ATAGGAGGCG ACCTGCTAGC CCCAGGATTC AAATCCAGCA TGCATCTCCG 
GAGAATGACA GGCACTCCAG AAAGTCTGAA AGATGTAGCA TCCAAAAACA GTCTAGGAAA 
GGCACAGCCT CTGATGCAGA CAGGGTTCTC CCACCATGCC TTTCTAGAAG GGGATACGCA 
ACCCCAAGAG CAACCGATCA ACCGGTCGTT AGGGGAAAGC ATCCCACTCG TTCACGGTCG 
AGCGAGCACT CTAGTGTCAG AACCCTGTGT TCTATGCACC ACCTTGCCCC CGGAGGGTCG 
GCGCCACCTT CTCCACTTCT GACAAGAACG CACCGACAAG GAAGCCCAAC CCAGTCTCCT 
CCAGCAGACA CATCCTTCGG CAGTCGCCGT GGAAGACAGC TCCCACAGGT GCCAGTTCGA 
AGCGGCAGTA TAGAACAAGC AAGCTTAGTA GTGGAGGAGC GAACGAGACA GATGAAAATG 
AAAGTTCACC GATTTAAGCA GACAACAGGG TCTGGGTCTA GTCAAGAACT TGACCACGAG 
CAATACTCCA AGAAGTCACG TTACATTATG GAGTACAACA TACATAAAGA TCAGTACAGA 
AGCTGTGATA ACGCGTCTGC CAAGTCTTCA GATAGTGATG TCAGTGATGT GTCCGCCATT 
TCCAGAGCCA GCAGTACCTC ACGCCTCAGC AGCACAAGCT TTATGTCAGA GCAGTCTGAG 
CGCCCCAGGG GTAGGATCAG TTCATTTACC CCCAAAATGC AAGGCAGACG GATGGGGACT 
TCAGGAAGAG CCATCATCAA GAGCACCAGT GTAAGTGGAG AGATATATAC ACTGGAACGT 
AATGACGGTA GCCAGTCGGA CACGGCCGTG GGTACCGTCG GAGCCGGTGG AAAGAAACGA 
AGATCCAGCC TGAGCGCCAA AGTGGTAGCC ATTGTGTCTC GAAGAAGCAG GAGCACGTCA 
CAGCTCAGCC AGACAGAGTC GGGCCACAAG AAGTTGAAAA GCACCATCCA GAGGAGTACG 
GAAACAGGAA TGGCAGCTGA AATGCGGAAG ATGGTGAGAC AGCCGAGCCG GGAGTCCACG 
GATGGCAGCA TCAACAGTTA TAGCTCGGAA GGAAACTTGA TATTTCCTGG AGTTCGAGTA 
GGACCCGACA GTCAGTTCAG TGATTTCCTT GATGGGTTGG GACCAGCGCA GCTCGTTGGC 
CGTCAGACGC TCGCCACCCC GGCCATGGGC GATATCCAAA TCGGGATGGA GGATAAGAAG 
GGTCAGTTGG AGGTTGAGGT TATCAGAGCC CGGAGCCTTA CACAAAAACC TGGTTCCAAA 
TCTACACCCG CTCCCTATGT GAAAGTATAT CTTTTGGAAA ATGGAGCCTG TATTGCCAAA 
AAGAAGACAA GAATTGCACG GAAAACTCTC GATCCTTTGT ATCAGCAGTC CCTGGTTTTT 
GATGAAAGTC CACAGGGTAA AGTTCTTCAG GTGATTGTCT GGGGTGACTA TGGAAGAATG 
GACCACAAAT GCTTTATGGG TGTGGCTCAA ATCTTGTTGG AAGAACTTGA TCTATCCAGC 
ATGGTGATTG GATGGTATAA ATTGTTCCCT CCGTCCTCAC TGGTGGATCC CACTCTCGCT 
CCCCTGACCC GCCGGGCTTC CCAATCATCT CTGGAAAGTT CGTCCGGGCC TCCCTGCATC 
CGGTCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rims1 ( XM_017596660.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017452149.1 Rattus norvegicus regulating synaptic membrane exocytosis 1 (Rims1), transcript variant X6, mRNA.

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CloneID	ORa62877
Clone ID Related Accession (Same CDS sequence)	XM_017596660.1
Accession Version	XM_017596660.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4866bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	regulating synaptic membrane exocytosis protein 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005108.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCTCGG CCGTGGGGCC CCGAGGTCCT CGCCCACCCA CGGTGCCTCC CCCTATGCAA 
GAACTGCCCG ACCTGAGCCA CCTGACCGAG GAGGAGAGGA ACATTATCAT GGCAGTGATG 
GACCGGCAGA AGGAAGAGGA GGAAAAAGAA GAGGCCATGC TCAAGTGTGT TGTCAGGGAC 
ATGGCGAAGC CTGCTGCCTG CAAAACACCA AGAAATGCTG AAAGCCAGCC CCATCAACCA 
CCACTGAACA TTTTCAGATG TGTCTGTGTT CCCAGAAAGC CAAGCAGCGA AGAGGGAGGC 
CCAGAAAGAG ACTGGAGATT GCATCAACAG TTTGAAAGCT ACAAGGAGCA AGTGAGAAAA 
ATCGGAGAGG AAGCGAGGCG TTACCAGGGC GAGCACAAGG ATGATGCCCC GACGTGTGGA 
ATCTGTCATA AGACAAAGTT TGCTGATGGA TGTGGCCATC TCTGCTCCTA TTGTCGCACC 
AAGTTCTGTG CACGCTGCGG AGGCCGTGTG TCTCTGCGAT CGAACAATGA GGACAAAGTG 
GTTATGTGGG TATGCAATTT ATGTCGAAAG CAACAAGAAA TCTTAACGAA ATCTGGAGCG 
TGGTTCTTTG GAAGTGGCCC TCAGCAGCCT AGTCAAGATG GGACTCTGAG TGACACGGCC 
ACAGGTGCTG GATCTGAGGT GCCAAGAGAA AAGAAAGCAA GGCTCCAAGA GCGATCAAGG 
TCTCAGACGC CCTTGAGTAC AGCAGCTGTC TCTTCCCAAG ACACTGCTAC CCCCGGTGCA 
CCGTTGGACA GGAACAAAGG GGCTGAGCCC TCACAGCAAG CCTTGGGTCC TGAACAGAAG 
CAGGCATCAA GATCAAGAAG CGAGCCACCG AGGGAAAGGA AGAAGGCTCC AGGGCTTTCA 
GAGCAGAATG GCAAGGGAGG CCAGAAGAGC GAGCGCAAAC GTGTCCCCAA GTCTGTGGTG 
CAACCCGGGG AAGGGATCGC GGATGAGAGG GAGAGGAAAG AGAGGCGGGA AACCCGCAGG 
TTGGAGAAAG GGCGCTCCCA GGACTACTCA GACCGGCCTG AGAAACGCGA CAATGGCAGG 
GTGGCGGAAG ACCAGAAGCA GAGGAAGGAG GAGGAGTACC AGACTAGGTA CCGCAGCGAC 
CCTAACCTGG CTCGCTACCC GGTGAAGGCG CCGCCAGAGG AGCAGCAGAT GCGCATGCAC 
GCCCGGGTGT CCCGAGCGAG GCACGAGCGG CGCCACAGCG ACGTGGCGCT CCCGCACACC 
GAGGCAGCTG CCGCCGCGCC GGCTGAGGCC ACGGCGGGCA AGCGCGCGCC GGCCACCGCC 
AGGGTCTCTC CCCCGGAGTC CCCGCGCGCA CGCGCGCCCG CCGCCCAGCC TCCCACCGAG 
CACGGGCCAC CGCCGCCGCG GCCAGTCCCG GGTCCCGCAG AGCCACCCGA GCCGCGCGTC 
CCCGAGCCGC TCCGTAAGCA GGGCCGCCTG GACCCGGCCT CGGCCGTGCT TCTGCGCAAG 
GCCAAGCGCG AGAAGGCGGA GAGCATGCTG CGGAACGACT CGCTGAGCTC CGATCAGTCC 
GAGTCCGTGC GGCCATCCCC GCCCAAGCCT CACCGGCCCA AGCGGGGAGG CAAGAGACGT 
CAGATGTCGG TGAGCAGCTC GGAGGAGGAG GGCGTGTCCA CACCGGAGTA CACGAGCTGC 
GAGGACGTGG AGCTGGAGAG CGAGAGCGTG AGCGAGAAAG GTGACTTGGA TTACTACTGG 
TTGGATCCCG CCACGTGGCA CAGCAGGGAA ACGTCGCCTA TCAGTTCGCA TCCTGTAACG 
TGGCAGCCGT CTAAAGAGGG AGATCGACTA ATCGGCCGTG TTATTCTTAA CAAAAGAACA 
ACCATGCCCA AAGAATCAGG TGCATTATTG GGTCTGAAGG TGGTTGGAGG AAAAATGACG 
GACTTAGGGC GCCTTGGTGC TTTCATCACC AAAGTAAAGA AGGGCAGCCT GGCAGACGTC 
GTCGGACACC TAAGAGCAGG GGACGAAGTC CTAGAGTGGA ATGGTAAACC CCTGCCGGGA 
GCAACAAACG AAGAAGTTTA CAACATTATC TTAGAATCAA AATCAGAACC TCAAGTTGAG 
ATTATTGTTT CAAGGCCTAT TGGTGACATC CCCAGGATCC CTGAGAGTTC CCATCCTCCC 
CTGGAGTCCA GTTCAAGTTC CTTTGAATCT CAGAAAATGG AAAGGCCTTC TATTTCTGTT 
ATTTCTCCAA CCAGCCCTGG AGCTCTGAAA GATGCCCCAC AAGTCTTACC AGGGCAACTC 
TCAGTGAAGC TATGGTATGA TAAAGTGGGG CACCAGCTGA TTGTAAATGT TCTACAAGCA 
ACAGATCTAC CCCCTAGAGT AGATGGCCGT CCCAGGAATC CCTATGTAAA AATGTATTTT 
CTTCCAGATA GAAGCGACAA AAGTAAAAGG AGAACCAAAA CAGTAAAGAA ACTTCTAGAG 
CCAAAATGGA ACCAGACATT TGTCTACTCA CACGTACATC GTAGAGATTT TCGAGAGCGA 
ATGTTAGAGA TTACCGTGTG GGACCAGCCG AGAGTACAGG ACGAAGAGAG TGAATTTCTT 
GGAGAGATCC TCATAGAGTT GGAAACAGCG CTTTTAGATG ATGAGCCCCA TTGGTATAAA 
CTCCAGACAC ATGACGAATC TTCACTACCT CTGCCTCAGC CATCACCGTT CATGCCCAGG 
CGGCATATTC ATGGAGAGAG CTCCAGCAAA AAGCTACAAA GATCTCAGCG AATCAGTGAT 
AGTGACATCT CAGATTATGA GGTTGATGAT GGTATTGGAG TAGTGCCTCC AGTGGGTTAT 
AGAGCTAGTG CTAGAGAGAG TAAAGCAACC ACGTTAACAG TGCCAGAGCA ACAAAGAACT 
ACACATCACC GCTCACGTTC CGTGTCTCCT CATCGCGGCG ATGATCAGGG AAGGCCTCGT 
TCACGTTTAC CAAATGTGCC ATTACAGAGG AGCTTAGATG AAATTCATCC AACACGAAGG 
TCACGTTCTC CAACCCGACA CCATGATGCC TCCCGAAGCC CGGCCGATCA CAGATCCAGA 
CATGTGGAAA GTCAATATTC GTCAGAGCCA GACAGTGAGC TTCTCATGCT GCCCAGAGCA 
AAACGAGGAC GAAGTGCAGA AAGCCTACAC ATGACCAGTG AACTGCAGCC CTCTCTTGAC 
AGGGCTAGGA GTGCTAGTAC CAACTGCTTG AGACCAGATA CTAGTTTGCA TTCACCAGAA 
CGAGAAAGGC ACTCCAGAAA GTCTGAAAGA TGTAGCATCC AAAAACAGTC TAGGAAAGGC 
ACAGCCTCTG ATGCAGACAG GGTTCTCCCA CCATGCCTTT CTAGAAGGGG ATACGCAACC 
CCAAGAGCAA CCGATCAACC GGTCGTTAGG GGAAAGCATC CCACTCGTTC ACGGTCGAGC 
GAGCACTCTA GTGTCAGAAC CCTGTGTTCT ATGCACCACC TTGCCCCCGG AGGGTCGGCG 
CCACCTTCTC CACTTCTGAC AAGAACGCAC CGACAAGGAA GCCCAACCCA GTCTCCTCCA 
GCAGACACAT CCTTCGGCAG TCGCCGTGGA AGACAGCTCC CACAGGTGCC AGTTCGAAGC 
GGCAGTATAG AACAAGCAAG CTTAGTAGTG GAGGAGCGAA CGAGACAGAT GAAAATGAAA 
GTTCACCGAT TTAAGCAGAC AACAGGGTCT GGGTCTAGTC AAGAACTTGA CCACGAGCAA 
TACTCCAAGA AGTCACGTTA CATTATGGAG TACAACATAC ATAAAGATCA GTACAGAAGC 
TGTGATAACG CGTCTGCCAA GTCTTCAGAT AGTGATGTCA GTGATGTGTC CGCCATTTCC 
AGAGCCAGCA GTACCTCACG CCTCAGCAGC ACAAGCTTTA TGTCAGAGCA GTCTGAGCGC 
CCCAGGGGTA GGATCAGTTC ATTTACCCCC AAAATGCAAG GCAGACGGAT GGGGACTTCA 
GGAAGAGCCA TCATCAAGAG CACCAGTGTA AGTGGAGAGA TATATACACT GGAACGTAAT 
GACGGTAGCC AGTCGGACAC GGCCGTGGGT ACCGTCGGAG CCGGTGGAAA GAAACGAAGA 
TCCAGCCTGA GCGCCAAAGT GGTAGCCATT GTGTCTCGAA GAAGCAGGAG CACGTCACAG 
CTCAGCCAGA CAGAGTCGGG CCACAAGAAG TTGAAAAGCA CCATCCAGAG GAGTACGGAA 
ACAGGAATGG CAGCTGAAAT GCGGAAGATG GTGAGACAGC CGAGCCGGGA GTCCACGGAT 
GGCAGCATCA ACAGTTATAG CTCGGAAGGA AACTTGATAT TTCCTGGAGT TCGAGTAGGA 
CCCGACAGTC AGTTCAGTGA TTTCCTTGAT GGGTTGGGAC CAGCGCAGCT CGTTGGCCGT 
CAGACGCTCG CCACCCCGGC CATGGGCGAT ATCCAAATCG GGATGGAGGA TAAGAAGGGT 
CAGTTGGAGG TTGAGGTTAT CAGAGCCCGG AGCCTTACAC AAAAACCTGG TTCCAAATCT 
ACACCCGCTC CCTATGTGAA AGTATATCTT TTGGAAAATG GAGCCTGTAT TGCCAAAAAG 
AAGACAAGAA TTGCACGGAA AACTCTCGAT CCTTTGTATC AGCAGTCCCT GGTTTTTGAT 
GAAAGTCCAC AGGGTAAAGT TCTTCAGGTG ATTGTCTGGG GTGACTATGG AAGAATGGAC 
CACAAATGCT TTATGGGTGT GGCTCAAATC TTGTTGGAAG AACTTGATCT ATCCAGCATG 
GTGATTGGAT GGTATAAATT GTTCCCTCCG TCCTCACTGG TGGATCCCAC TCTCGCTCCC 
CTGACCCGCC GGGCTTCCCA ATCATCTCTG GAAAGTTCGT CCGGGCCTCC CTGCATCCGG 
TCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017452149.1
CDS	315..5180
Translation	MSSAVGPRGPRPPTVPPPMQELPDLSHLTEEERNIIMAVMDRQKEEEEKEEAMLKCVVRDMAKPAACKTPRNAESQPHQPPLNIFRCVCVPRKPSSEEGGPERDWRLHQQFESYKEQVRKIGEEARRYQGEHKDDAPTCGICHKTKFADGCGHLCSYCRTKFCARCGGRVSLRSNNEDKVVMWVCNLCRKQQEILTKSGAWFFGSGPQQPSQDGTLSDTATGAGSEVPREKKARLQERSRSQTPLSTAAVSSQDTATPGAPLDRNKGAEPSQQALGPEQKQASRSRSEPPRERKKAPGLSEQNGKGGQKSERKRVPKSVVQPGEGIADERERKERRETRRLEKGRSQDYSDRPEKRDNGRVAEDQKQRKEEEYQTRYRSDPNLARYPVKAPPEEQQMRMHARVSRARHERRHSDVALPHTEAAAAAPAEATAGKRAPATARVSPPESPRARAPAAQPPTEHGPPPPRPVPGPAEPPEPRVPEPLRKQGRLDPASAVLLRKAKREKAESMLRNDSLSSDQSESVRPSPPKPHRPKRGGKRRQMSVSSSEEEGVSTPEYTSCEDVELESESVSEKGDLDYYWLDPATWHSRETSPISSHPVTWQPSKEGDRLIGRVILNKRTTMPKESGALLGLKVVGGKMTDLGRLGAFITKVKKGSLADVVGHLRAGDEVLEWNGKPLPGATNEEVYNIILESKSEPQVEIIVSRPIGDIPRIPESSHPPLESSSSSFESQKMERPSISVISPTSPGALKDAPQVLPGQLSVKLWYDKVGHQLIVNVLQATDLPPRVDGRPRNPYVKMYFLPDRSDKSKRRTKTVKKLLEPKWNQTFVYSHVHRRDFRERMLEITVWDQPRVQDEESEFLGEILIELETALLDDEPHWYKLQTHDESSLPLPQPSPFMPRRHIHGESSSKKLQRSQRISDSDISDYEVDDGIGVVPPVGYRASARESKATTLTVPEQQRTTHHRSRSVSPHRGDDQGRPRSRLPNVPLQRSLDEIHPTRRSRSPTRHHDASRSPADHRSRHVESQYSSEPDSELLMLPRAKRGRSAESLHMTSELQPSLDRARSASTNCLRPDTSLHSPERERHSRKSERCSIQKQSRKGTASDADRVLPPCLSRRGYATPRATDQPVVRGKHPTRSRSSEHSSVRTLCSMHHLAPGGSAPPSPLLTRTHRQGSPTQSPPADTSFGSRRGRQLPQVPVRSGSIEQASLVVEERTRQMKMKVHRFKQTTGSGSSQELDHEQYSKKSRYIMEYNIHKDQYRSCDNASAKSSDSDVSDVSAISRASSTSRLSSTSFMSEQSERPRGRISSFTPKMQGRRMGTSGRAIIKSTSVSGEIYTLERNDGSQSDTAVGTVGAGGKKRRSSLSAKVVAIVSRRSRSTSQLSQTESGHKKLKSTIQRSTETGMAAEMRKMVRQPSRESTDGSINSYSSEGNLIFPGVRVGPDSQFSDFLDGLGPAQLVGRQTLATPAMGDIQIGMEDKKGQLEVEVIRARSLTQKPGSKSTPAPYVKVYLLENGACIAKKKTRIARKTLDPLYQQSLVFDESPQGKVLQVIVWGDYGRMDHKCFMGVAQILLEELDLSSMVIGWYKLFPPSSLVDPTLAPLTRRASQSSLESSSGPPCIRS

Target ORF information:

RefSeq Version	XM_017596660.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus regulating synaptic membrane exocytosis 1 (Rims1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017596660.1

ORF Insert Sequence:

ATGTCCTCGG CCGTGGGGCC CCGAGGTCCT CGCCCACCCA CGGTGCCTCC CCCTATGCAA 
GAACTGCCCG ACCTGAGCCA CCTGACCGAG GAGGAGAGGA ACATTATCAT GGCAGTGATG 
GACCGGCAGA AGGAAGAGGA GGAAAAAGAA GAGGCCATGC TCAAGTGTGT TGTCAGGGAC 
ATGGCGAAGC CTGCTGCCTG CAAAACACCA AGAAATGCTG AAAGCCAGCC CCATCAACCA 
CCACTGAACA TTTTCAGATG TGTCTGTGTT CCCAGAAAGC CAAGCAGCGA AGAGGGAGGC 
CCAGAAAGAG ACTGGAGATT GCATCAACAG TTTGAAAGCT ACAAGGAGCA AGTGAGAAAA 
ATCGGAGAGG AAGCGAGGCG TTACCAGGGC GAGCACAAGG ATGATGCCCC GACGTGTGGA 
ATCTGTCATA AGACAAAGTT TGCTGATGGA TGTGGCCATC TCTGCTCCTA TTGTCGCACC 
AAGTTCTGTG CACGCTGCGG AGGCCGTGTG TCTCTGCGAT CGAACAATGA GGACAAAGTG 
GTTATGTGGG TATGCAATTT ATGTCGAAAG CAACAAGAAA TCTTAACGAA ATCTGGAGCG 
TGGTTCTTTG GAAGTGGCCC TCAGCAGCCT AGTCAAGATG GGACTCTGAG TGACACGGCC 
ACAGGTGCTG GATCTGAGGT GCCAAGAGAA AAGAAAGCAA GGCTCCAAGA GCGATCAAGG 
TCTCAGACGC CCTTGAGTAC AGCAGCTGTC TCTTCCCAAG ACACTGCTAC CCCCGGTGCA 
CCGTTGGACA GGAACAAAGG GGCTGAGCCC TCACAGCAAG CCTTGGGTCC TGAACAGAAG 
CAGGCATCAA GATCAAGAAG CGAGCCACCG AGGGAAAGGA AGAAGGCTCC AGGGCTTTCA 
GAGCAGAATG GCAAGGGAGG CCAGAAGAGC GAGCGCAAAC GTGTCCCCAA GTCTGTGGTG 
CAACCCGGGG AAGGGATCGC GGATGAGAGG GAGAGGAAAG AGAGGCGGGA AACCCGCAGG 
TTGGAGAAAG GGCGCTCCCA GGACTACTCA GACCGGCCTG AGAAACGCGA CAATGGCAGG 
GTGGCGGAAG ACCAGAAGCA GAGGAAGGAG GAGGAGTACC AGACTAGGTA CCGCAGCGAC 
CCTAACCTGG CTCGCTACCC GGTGAAGGCG CCGCCAGAGG AGCAGCAGAT GCGCATGCAC 
GCCCGGGTGT CCCGAGCGAG GCACGAGCGG CGCCACAGCG ACGTGGCGCT CCCGCACACC 
GAGGCAGCTG CCGCCGCGCC GGCTGAGGCC ACGGCGGGCA AGCGCGCGCC GGCCACCGCC 
AGGGTCTCTC CCCCGGAGTC CCCGCGCGCA CGCGCGCCCG CCGCCCAGCC TCCCACCGAG 
CACGGGCCAC CGCCGCCGCG GCCAGTCCCG GGTCCCGCAG AGCCACCCGA GCCGCGCGTC 
CCCGAGCCGC TCCGTAAGCA GGGCCGCCTG GACCCGGCCT CGGCCGTGCT TCTGCGCAAG 
GCCAAGCGCG AGAAGGCGGA GAGCATGCTG CGGAACGACT CGCTGAGCTC CGATCAGTCC 
GAGTCCGTGC GGCCATCCCC GCCCAAGCCT CACCGGCCCA AGCGGGGAGG CAAGAGACGT 
CAGATGTCGG TGAGCAGCTC GGAGGAGGAG GGCGTGTCCA CACCGGAGTA CACGAGCTGC 
GAGGACGTGG AGCTGGAGAG CGAGAGCGTG AGCGAGAAAG GTGACTTGGA TTACTACTGG 
TTGGATCCCG CCACGTGGCA CAGCAGGGAA ACGTCGCCTA TCAGTTCGCA TCCTGTAACG 
TGGCAGCCGT CTAAAGAGGG AGATCGACTA ATCGGCCGTG TTATTCTTAA CAAAAGAACA 
ACCATGCCCA AAGAATCAGG TGCATTATTG GGTCTGAAGG TGGTTGGAGG AAAAATGACG 
GACTTAGGGC GCCTTGGTGC TTTCATCACC AAAGTAAAGA AGGGCAGCCT GGCAGACGTC 
GTCGGACACC TAAGAGCAGG GGACGAAGTC CTAGAGTGGA ATGGTAAACC CCTGCCGGGA 
GCAACAAACG AAGAAGTTTA CAACATTATC TTAGAATCAA AATCAGAACC TCAAGTTGAG 
ATTATTGTTT CAAGGCCTAT TGGTGACATC CCCAGGATCC CTGAGAGTTC CCATCCTCCC 
CTGGAGTCCA GTTCAAGTTC CTTTGAATCT CAGAAAATGG AAAGGCCTTC TATTTCTGTT 
ATTTCTCCAA CCAGCCCTGG AGCTCTGAAA GATGCCCCAC AAGTCTTACC AGGGCAACTC 
TCAGTGAAGC TATGGTATGA TAAAGTGGGG CACCAGCTGA TTGTAAATGT TCTACAAGCA 
ACAGATCTAC CCCCTAGAGT AGATGGCCGT CCCAGGAATC CCTATGTAAA AATGTATTTT 
CTTCCAGATA GAAGCGACAA AAGTAAAAGG AGAACCAAAA CAGTAAAGAA ACTTCTAGAG 
CCAAAATGGA ACCAGACATT TGTCTACTCA CACGTACATC GTAGAGATTT TCGAGAGCGA 
ATGTTAGAGA TTACCGTGTG GGACCAGCCG AGAGTACAGG ACGAAGAGAG TGAATTTCTT 
GGAGAGATCC TCATAGAGTT GGAAACAGCG CTTTTAGATG ATGAGCCCCA TTGGTATAAA 
CTCCAGACAC ATGACGAATC TTCACTACCT CTGCCTCAGC CATCACCGTT CATGCCCAGG 
CGGCATATTC ATGGAGAGAG CTCCAGCAAA AAGCTACAAA GATCTCAGCG AATCAGTGAT 
AGTGACATCT CAGATTATGA GGTTGATGAT GGTATTGGAG TAGTGCCTCC AGTGGGTTAT 
AGAGCTAGTG CTAGAGAGAG TAAAGCAACC ACGTTAACAG TGCCAGAGCA ACAAAGAACT 
ACACATCACC GCTCACGTTC CGTGTCTCCT CATCGCGGCG ATGATCAGGG AAGGCCTCGT 
TCACGTTTAC CAAATGTGCC ATTACAGAGG AGCTTAGATG AAATTCATCC AACACGAAGG 
TCACGTTCTC CAACCCGACA CCATGATGCC TCCCGAAGCC CGGCCGATCA CAGATCCAGA 
CATGTGGAAA GTCAATATTC GTCAGAGCCA GACAGTGAGC TTCTCATGCT GCCCAGAGCA 
AAACGAGGAC GAAGTGCAGA AAGCCTACAC ATGACCAGTG AACTGCAGCC CTCTCTTGAC 
AGGGCTAGGA GTGCTAGTAC CAACTGCTTG AGACCAGATA CTAGTTTGCA TTCACCAGAA 
CGAGAAAGGC ACTCCAGAAA GTCTGAAAGA TGTAGCATCC AAAAACAGTC TAGGAAAGGC 
ACAGCCTCTG ATGCAGACAG GGTTCTCCCA CCATGCCTTT CTAGAAGGGG ATACGCAACC 
CCAAGAGCAA CCGATCAACC GGTCGTTAGG GGAAAGCATC CCACTCGTTC ACGGTCGAGC 
GAGCACTCTA GTGTCAGAAC CCTGTGTTCT ATGCACCACC TTGCCCCCGG AGGGTCGGCG 
CCACCTTCTC CACTTCTGAC AAGAACGCAC CGACAAGGAA GCCCAACCCA GTCTCCTCCA 
GCAGACACAT CCTTCGGCAG TCGCCGTGGA AGACAGCTCC CACAGGTGCC AGTTCGAAGC 
GGCAGTATAG AACAAGCAAG CTTAGTAGTG GAGGAGCGAA CGAGACAGAT GAAAATGAAA 
GTTCACCGAT TTAAGCAGAC AACAGGGTCT GGGTCTAGTC AAGAACTTGA CCACGAGCAA 
TACTCCAAGA AGTCACGTTA CATTATGGAG TACAACATAC ATAAAGATCA GTACAGAAGC 
TGTGATAACG CGTCTGCCAA GTCTTCAGAT AGTGATGTCA GTGATGTGTC CGCCATTTCC 
AGAGCCAGCA GTACCTCACG CCTCAGCAGC ACAAGCTTTA TGTCAGAGCA GTCTGAGCGC 
CCCAGGGGTA GGATCAGTTC ATTTACCCCC AAAATGCAAG GCAGACGGAT GGGGACTTCA 
GGAAGAGCCA TCATCAAGAG CACCAGTGTA AGTGGAGAGA TATATACACT GGAACGTAAT 
GACGGTAGCC AGTCGGACAC GGCCGTGGGT ACCGTCGGAG CCGGTGGAAA GAAACGAAGA 
TCCAGCCTGA GCGCCAAAGT GGTAGCCATT GTGTCTCGAA GAAGCAGGAG CACGTCACAG 
CTCAGCCAGA CAGAGTCGGG CCACAAGAAG TTGAAAAGCA CCATCCAGAG GAGTACGGAA 
ACAGGAATGG CAGCTGAAAT GCGGAAGATG GTGAGACAGC CGAGCCGGGA GTCCACGGAT 
GGCAGCATCA ACAGTTATAG CTCGGAAGGA AACTTGATAT TTCCTGGAGT TCGAGTAGGA 
CCCGACAGTC AGTTCAGTGA TTTCCTTGAT GGGTTGGGAC CAGCGCAGCT CGTTGGCCGT 
CAGACGCTCG CCACCCCGGC CATGGGCGAT ATCCAAATCG GGATGGAGGA TAAGAAGGGT 
CAGTTGGAGG TTGAGGTTAT CAGAGCCCGG AGCCTTACAC AAAAACCTGG TTCCAAATCT 
ACACCCGCTC CCTATGTGAA AGTATATCTT TTGGAAAATG GAGCCTGTAT TGCCAAAAAG 
AAGACAAGAA TTGCACGGAA AACTCTCGAT CCTTTGTATC AGCAGTCCCT GGTTTTTGAT 
GAAAGTCCAC AGGGTAAAGT TCTTCAGGTG ATTGTCTGGG GTGACTATGG AAGAATGGAC 
CACAAATGCT TTATGGGTGT GGCTCAAATC TTGTTGGAAG AACTTGATCT ATCCAGCATG 
GTGATTGGAT GGTATAAATT GTTCCCTCCG TCCTCACTGG TGGATCCCAC TCTCGCTCCC 
CTGACCCGCC GGGCTTCCCA ATCATCTCTG GAAAGTTCGT CCGGGCCTCC CTGCATCCGG 
TCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rims1 ( XM_017596661.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017452150.1 Rattus norvegicus regulating synaptic membrane exocytosis 1 (Rims1), transcript variant X8, mRNA.

Price & Availability↑

CloneID	ORa62878
Clone ID Related Accession (Same CDS sequence)	XM_017596661.1
Accession Version	XM_017596661.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4800bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	regulating synaptic membrane exocytosis protein 1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005108.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCTCGG CCGTGGGGCC CCGAGGTCCT CGCCCACCCA CGGTGCCTCC CCCTATGCAA 
GAACTGCCCG ACCTGAGCCA CCTGACCGAG GAGGAGAGGA ACATTATCAT GGCAGTGATG 
GACCGGCAGA AGGAAGAGGA GGAAAAAGAA GAGGCCATGC TCAAGAGATT GCATCAACAG 
TTTGAAAGCT ACAAGGAGCA AGTGAGAAAA ATCGGAGAGG AAGCGAGGCG TTACCAGGGC 
GAGCACAAGG ATGATGCCCC GACGTGTGGA ATCTGTCATA AGACAAAGTT TGCTGATGGA 
TGTGGCCATC TCTGCTCCTA TTGTCGCACC AAGTTCTGTG CACGCTGCGG AGGCCGTGTG 
TCTCTGCGAT CGAACAATGA GGACAAAGTG GTTATGTGGG TATGCAATTT ATGTCGAAAG 
CAACAAGAAA TCTTAACGAA ATCTGGAGCG TGGTTCTTTG GAAGTGGCCC TCAGCAGCCT 
AGTCAAGATG GGACTCTGAG TGACACGGCC ACAGGTGCTG GATCTGAGGT GCCAAGAGAA 
AAGAAAGCAA GGCTCCAAGA GCGATCAAGG TCTCAGACGC CCTTGAGTAC AGCAGCTGTC 
TCTTCCCAAG ACACTGCTAC CCCCGGTGCA CCGTTGGACA GGAACAAAGG GGCTGAGCCC 
TCACAGCAAG CCTTGGGTCC TGAACAGAAG CAGGCATCAA GATCAAGAAG CGAGCCACCG 
AGGGAAAGGA AGAAGGCTCC AGGGCTTTCA GAGCAGAATG GCAAGGGAGG CCAGAAGAGC 
GAGCGCAAAC GTGTCCCCAA GTCTGTGGTG CAACCCGGGG AAGGGATCGC GGATGAGAGG 
GAGAGGAAAG AGAGGCGGGA AACCCGCAGG TTGGAGAAAG GGCGCTCCCA GGACTACTCA 
GACCGGCCTG AGAAACGCGA CAATGGCAGG GTGGCGGAAG ACCAGAAGCA GAGGAAGGAG 
GAGGAGTACC AGACTAGGTA CCGCAGCGAC CCTAACCTGG CTCGCTACCC GGTGAAGGCG 
CCGCCAGAGG AGCAGCAGAT GCGCATGCAC GCCCGGGTGT CCCGAGCGAG GCACGAGCGG 
CGCCACAGCG ACGTGGCGCT CCCGCACACC GAGGCAGCTG CCGCCGCGCC GGCTGAGGCC 
ACGGCGGGCA AGCGCGCGCC GGCCACCGCC AGGGTCTCTC CCCCGGAGTC CCCGCGCGCA 
CGCGCGCCCG CCGCCCAGCC TCCCACCGAG CACGGGCCAC CGCCGCCGCG GCCAGTCCCG 
GGTCCCGCAG AGCCACCCGA GCCGCGCGTC CCCGAGCCGC TCCGTAAGCA GGGCCGCCTG 
GACCCGGCCT CGGCCGTGCT TCTGCGCAAG GCCAAGCGCG AGAAGGCGGA GAGCATGCTG 
CGGAACGACT CGCTGAGCTC CGATCAGTCC GAGTCCGTGC GGCCATCCCC GCCCAAGCCT 
CACCGGCCCA AGCGGGGAGG CAAGAGACGT CAGATGTCGG TGAGCAGCTC GGAGGAGGAG 
GGCGTGTCCA CACCGGAGTA CACGAGCTGC GAGGACGTGG AGCTGGAGAG CGAGAGCGTG 
AGCGAGAAAG GTGACTTGGA TTACTACTGG TTGGATCCCG CCACGTGGCA CAGCAGGGAA 
ACGTCGCCTA TCAGTTCGCA TCCTGTAACG TGGCAGCCGT CTAAAGAGGG AGATCGACTA 
ATCGGCCGTG TTATTCTTAA CAAAAGAACA ACCATGCCCA AAGAATCAGG TGCATTATTG 
GGTCTGAAGG TGGTTGGAGG AAAAATGACG GACTTAGGGC GCCTTGGTGC TTTCATCACC 
AAAGTAAAGA AGGGCAGCCT GGCAGACGTC GTCGGACACC TAAGAGCAGG GGACGAAGTC 
CTAGAGTGGA ATGGTAAACC CCTGCCGGGA GCAACAAACG AAGAAGTTTA CAACATTATC 
TTAGAATCAA AATCAGAACC TCAAGTTGAG ATTATTGTTT CAAGGCCTAT TGGTGACATC 
CCCAGGATCC CTGAGAGTTC CCATCCTCCC CTGGAGTCCA GTTCAAGTTC CTTTGAATCT 
CAGAAAATGG AAAGGCCTTC TATTTCTGTT ATTTCTCCAA CCAGCCCTGG AGCTCTGAAA 
GATGCCCCAC AAGTCTTACC AGGGCAACTC TCAGTGAAGC TATGGTATGA TAAAGTGGGG 
CACCAGCTGA TTGTAAATGT TCTACAAGCA ACAGATCTAC CCCCTAGAGT AGATGGCCGT 
CCCAGGAATC CCTATGTAAA AATGTATTTT CTTCCAGATA GAAGCGACAA AAGTAAAAGG 
AGAACCAAAA CAGTAAAGAA ACTTCTAGAG CCAAAATGGA ACCAGACATT TGTCTACTCA 
CACGTACATC GTAGAGATTT TCGAGAGCGA ATGTTAGAGA TTACCGTGTG GGACCAGCCG 
AGAGTACAGG ACGAAGAGAG TGAATTTCTT GGAGAGATCC TCATAGAGTT GGAAACAGCG 
CTTTTAGATG ATGAGCCCCA TTGGTATAAA CTCCAGACAC ATGACGAATC TTCACTACCT 
CTGCCTCAGC CATCACCGTT CATGCCCAGG CGGCATATTC ATGGAGAGAG CTCCAGCAAA 
AAGCTACAAA GATCTCAGCG AATCAGTGAT AGTGACATCT CAGATTATGA GGTTGATGAT 
GGTATTGGAG TAGTGCCTCC AGTGGGTTAT AGAGCTAGTG CTAGAGAGAG TAAAGCAACC 
ACGTTAACAG TGCCAGAGCA ACAAAGAACT ACACATCACC GCTCACGTTC CGTGTCTCCT 
CATCGCGGCG ATGATCAGGG AAGGCCTCGT TCACGTTTAC CAAATGTGCC ATTACAGAGG 
AGCTTAGATG AAATTCATCC AACACGAAGG TCACGTTCTC CAACCCGACA CCATGATGCC 
TCCCGAAGCC CGGCCGATCA CAGATCCAGA CATGTGGAAA GTCAATATTC GTCAGAGCCA 
GACAGTGAGC TTCTCATGCT GCCCAGAGCA AAACGAGGAC GAAGTGCAGA AAGCCTACAC 
ATGACCAGTG AACTGCAGCC CTCTCTTGAC AGGGCTAGGA GTGCTAGTAC CAACTGCTTG 
AGACCAGATA CTAGTTTGCA TTCACCAGAA CGAGAAAGGG GTAGATGGTC CCCCTCCCTA 
GATAGGAGGC GACCTGCTAG CCCCAGGATT CAAATCCAGC ATGCATCTCC GGAGAATGAC 
AGGCACTCCA GAAAGTCTGA AAGATGTAGC ATCCAAAAAC AGTCTAGGAA AGGCACAGCC 
TCTGATGCAG ACAGGGTTCT CCCACCATGC CTTTCTAGAA GGGGATACGC AACCCCAAGA 
GCAACCGATC AACCGGTCGT TAGGGGAAAG CATCCCACTC GTTCACGGTC GAGCGAGCAC 
TCTAGTGTCA GAACCCTGTG TTCTATGCAC CACCTTGCCC CCGGAGGGTC GGCGCCACCT 
TCTCCACTTC TGACAAGAAC GCACCGACAA GGAAGCCCAA CCCAGTCTCC TCCAGCAGAC 
ACATCCTTCG GCAGTCGCCG TGGAAGACAG CTCCCACAGG TGCCAGTTCG AAGCGGCAGT 
ATAGAACAAG CAAGCTTAGT AGTGGAGGAG CGAACGAGAC AGATGAAAAT GAAAGTTCAC 
CGATTTAAGC AGACAACAGG GTCTGGGTCT AGTCAAGAAC TTGACCACGA GCAATACTCC 
AAGAAGTCAC GTTACATTAT GGAGTACAAC ATACATAAAG ATCAGTACAG AAGCTGTGAT 
AACGCGTCTG CCAAGTCTTC AGATAGTGAT GTCAGTGATG TGTCCGCCAT TTCCAGAGCC 
AGCAGTACCT CACGCCTCAG CAGCACAAGC TTTATGTCAG AGCAGTCTGA GCGCCCCAGG 
GGTAGGATCA GTTCATTTAC CCCCAAAATG CAAGGCAGAC GGATGGGGAC TTCAGGAAGA 
GCCATCATCA AGAGCACCAG TGTAAGTGGA GAGATATATA CACTGGAACG TAATGACGGT 
AGCCAGTCGG ACACGGCCGT GGGTACCGTC GGAGCCGGTG GAAAGAAACG AAGATCCAGC 
CTGAGCGCCA AAGTGGTAGC CATTGTGTCT CGAAGAAGCA GGAGCACGTC ACAGCTCAGC 
CAGACAGAGT CGGGCCACAA GAAGTTGAAA AGCACCATCC AGAGGAGTAC GGAAACAGGA 
ATGGCAGCTG AAATGCGGAA GATGGTGAGA CAGCCGAGCC GGGAGTCCAC GGATGGCAGC 
ATCAACAGTT ATAGCTCGGA AGGAAACTTG ATATTTCCTG GAGTTCGAGT AGGACCCGAC 
AGTCAGTTCA GTGATTTCCT TGATGGGTTG GGACCAGCGC AGCTCGTTGG CCGTCAGACG 
CTCGCCACCC CGGCCATGGG CGATATCCAA ATCGGGATGG AGGATAAGAA GGGTCAGTTG 
GAGGTTGAGG TTATCAGAGC CCGGAGCCTT ACACAAAAAC CTGGTTCCAA ATCTACACCC 
GCTCCCTATG TGAAAGTATA TCTTTTGGAA AATGGAGCCT GTATTGCCAA AAAGAAGACA 
AGAATTGCAC GGAAAACTCT CGATCCTTTG TATCAGCAGT CCCTGGTTTT TGATGAAAGT 
CCACAGGGTA AAGTTCTTCA GGTGATTGTC TGGGGTGACT ATGGAAGAAT GGACCACAAA 
TGCTTTATGG GTGTGGCTCA AATCTTGTTG GAAGAACTTG ATCTATCCAG CATGGTGATT 
GGATGGTATA AATTGTTCCC TCCGTCCTCA CTGGTGGATC CCACTCTCGC TCCCCTGACC 
CGCCGGGCTT CCCAATCATC TCTGGAAAGT TCGTCCGGGC CTCCCTGCAT CCGGTCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017452150.1
CDS	314..5113
Translation	MSSAVGPRGPRPPTVPPPMQELPDLSHLTEEERNIIMAVMDRQKEEEEKEEAMLKRLHQQFESYKEQVRKIGEEARRYQGEHKDDAPTCGICHKTKFADGCGHLCSYCRTKFCARCGGRVSLRSNNEDKVVMWVCNLCRKQQEILTKSGAWFFGSGPQQPSQDGTLSDTATGAGSEVPREKKARLQERSRSQTPLSTAAVSSQDTATPGAPLDRNKGAEPSQQALGPEQKQASRSRSEPPRERKKAPGLSEQNGKGGQKSERKRVPKSVVQPGEGIADERERKERRETRRLEKGRSQDYSDRPEKRDNGRVAEDQKQRKEEEYQTRYRSDPNLARYPVKAPPEEQQMRMHARVSRARHERRHSDVALPHTEAAAAAPAEATAGKRAPATARVSPPESPRARAPAAQPPTEHGPPPPRPVPGPAEPPEPRVPEPLRKQGRLDPASAVLLRKAKREKAESMLRNDSLSSDQSESVRPSPPKPHRPKRGGKRRQMSVSSSEEEGVSTPEYTSCEDVELESESVSEKGDLDYYWLDPATWHSRETSPISSHPVTWQPSKEGDRLIGRVILNKRTTMPKESGALLGLKVVGGKMTDLGRLGAFITKVKKGSLADVVGHLRAGDEVLEWNGKPLPGATNEEVYNIILESKSEPQVEIIVSRPIGDIPRIPESSHPPLESSSSSFESQKMERPSISVISPTSPGALKDAPQVLPGQLSVKLWYDKVGHQLIVNVLQATDLPPRVDGRPRNPYVKMYFLPDRSDKSKRRTKTVKKLLEPKWNQTFVYSHVHRRDFRERMLEITVWDQPRVQDEESEFLGEILIELETALLDDEPHWYKLQTHDESSLPLPQPSPFMPRRHIHGESSSKKLQRSQRISDSDISDYEVDDGIGVVPPVGYRASARESKATTLTVPEQQRTTHHRSRSVSPHRGDDQGRPRSRLPNVPLQRSLDEIHPTRRSRSPTRHHDASRSPADHRSRHVESQYSSEPDSELLMLPRAKRGRSAESLHMTSELQPSLDRARSASTNCLRPDTSLHSPERERGRWSPSLDRRRPASPRIQIQHASPENDRHSRKSERCSIQKQSRKGTASDADRVLPPCLSRRGYATPRATDQPVVRGKHPTRSRSSEHSSVRTLCSMHHLAPGGSAPPSPLLTRTHRQGSPTQSPPADTSFGSRRGRQLPQVPVRSGSIEQASLVVEERTRQMKMKVHRFKQTTGSGSSQELDHEQYSKKSRYIMEYNIHKDQYRSCDNASAKSSDSDVSDVSAISRASSTSRLSSTSFMSEQSERPRGRISSFTPKMQGRRMGTSGRAIIKSTSVSGEIYTLERNDGSQSDTAVGTVGAGGKKRRSSLSAKVVAIVSRRSRSTSQLSQTESGHKKLKSTIQRSTETGMAAEMRKMVRQPSRESTDGSINSYSSEGNLIFPGVRVGPDSQFSDFLDGLGPAQLVGRQTLATPAMGDIQIGMEDKKGQLEVEVIRARSLTQKPGSKSTPAPYVKVYLLENGACIAKKKTRIARKTLDPLYQQSLVFDESPQGKVLQVIVWGDYGRMDHKCFMGVAQILLEELDLSSMVIGWYKLFPPSSLVDPTLAPLTRRASQSSLESSSGPPCIRS

Target ORF information:

RefSeq Version	XM_017596661.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus regulating synaptic membrane exocytosis 1 (Rims1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017596661.1

ORF Insert Sequence:

ATGTCCTCGG CCGTGGGGCC CCGAGGTCCT CGCCCACCCA CGGTGCCTCC CCCTATGCAA 
GAACTGCCCG ACCTGAGCCA CCTGACCGAG GAGGAGAGGA ACATTATCAT GGCAGTGATG 
GACCGGCAGA AGGAAGAGGA GGAAAAAGAA GAGGCCATGC TCAAGAGATT GCATCAACAG 
TTTGAAAGCT ACAAGGAGCA AGTGAGAAAA ATCGGAGAGG AAGCGAGGCG TTACCAGGGC 
GAGCACAAGG ATGATGCCCC GACGTGTGGA ATCTGTCATA AGACAAAGTT TGCTGATGGA 
TGTGGCCATC TCTGCTCCTA TTGTCGCACC AAGTTCTGTG CACGCTGCGG AGGCCGTGTG 
TCTCTGCGAT CGAACAATGA GGACAAAGTG GTTATGTGGG TATGCAATTT ATGTCGAAAG 
CAACAAGAAA TCTTAACGAA ATCTGGAGCG TGGTTCTTTG GAAGTGGCCC TCAGCAGCCT 
AGTCAAGATG GGACTCTGAG TGACACGGCC ACAGGTGCTG GATCTGAGGT GCCAAGAGAA 
AAGAAAGCAA GGCTCCAAGA GCGATCAAGG TCTCAGACGC CCTTGAGTAC AGCAGCTGTC 
TCTTCCCAAG ACACTGCTAC CCCCGGTGCA CCGTTGGACA GGAACAAAGG GGCTGAGCCC 
TCACAGCAAG CCTTGGGTCC TGAACAGAAG CAGGCATCAA GATCAAGAAG CGAGCCACCG 
AGGGAAAGGA AGAAGGCTCC AGGGCTTTCA GAGCAGAATG GCAAGGGAGG CCAGAAGAGC 
GAGCGCAAAC GTGTCCCCAA GTCTGTGGTG CAACCCGGGG AAGGGATCGC GGATGAGAGG 
GAGAGGAAAG AGAGGCGGGA AACCCGCAGG TTGGAGAAAG GGCGCTCCCA GGACTACTCA 
GACCGGCCTG AGAAACGCGA CAATGGCAGG GTGGCGGAAG ACCAGAAGCA GAGGAAGGAG 
GAGGAGTACC AGACTAGGTA CCGCAGCGAC CCTAACCTGG CTCGCTACCC GGTGAAGGCG 
CCGCCAGAGG AGCAGCAGAT GCGCATGCAC GCCCGGGTGT CCCGAGCGAG GCACGAGCGG 
CGCCACAGCG ACGTGGCGCT CCCGCACACC GAGGCAGCTG CCGCCGCGCC GGCTGAGGCC 
ACGGCGGGCA AGCGCGCGCC GGCCACCGCC AGGGTCTCTC CCCCGGAGTC CCCGCGCGCA 
CGCGCGCCCG CCGCCCAGCC TCCCACCGAG CACGGGCCAC CGCCGCCGCG GCCAGTCCCG 
GGTCCCGCAG AGCCACCCGA GCCGCGCGTC CCCGAGCCGC TCCGTAAGCA GGGCCGCCTG 
GACCCGGCCT CGGCCGTGCT TCTGCGCAAG GCCAAGCGCG AGAAGGCGGA GAGCATGCTG 
CGGAACGACT CGCTGAGCTC CGATCAGTCC GAGTCCGTGC GGCCATCCCC GCCCAAGCCT 
CACCGGCCCA AGCGGGGAGG CAAGAGACGT CAGATGTCGG TGAGCAGCTC GGAGGAGGAG 
GGCGTGTCCA CACCGGAGTA CACGAGCTGC GAGGACGTGG AGCTGGAGAG CGAGAGCGTG 
AGCGAGAAAG GTGACTTGGA TTACTACTGG TTGGATCCCG CCACGTGGCA CAGCAGGGAA 
ACGTCGCCTA TCAGTTCGCA TCCTGTAACG TGGCAGCCGT CTAAAGAGGG AGATCGACTA 
ATCGGCCGTG TTATTCTTAA CAAAAGAACA ACCATGCCCA AAGAATCAGG TGCATTATTG 
GGTCTGAAGG TGGTTGGAGG AAAAATGACG GACTTAGGGC GCCTTGGTGC TTTCATCACC 
AAAGTAAAGA AGGGCAGCCT GGCAGACGTC GTCGGACACC TAAGAGCAGG GGACGAAGTC 
CTAGAGTGGA ATGGTAAACC CCTGCCGGGA GCAACAAACG AAGAAGTTTA CAACATTATC 
TTAGAATCAA AATCAGAACC TCAAGTTGAG ATTATTGTTT CAAGGCCTAT TGGTGACATC 
CCCAGGATCC CTGAGAGTTC CCATCCTCCC CTGGAGTCCA GTTCAAGTTC CTTTGAATCT 
CAGAAAATGG AAAGGCCTTC TATTTCTGTT ATTTCTCCAA CCAGCCCTGG AGCTCTGAAA 
GATGCCCCAC AAGTCTTACC AGGGCAACTC TCAGTGAAGC TATGGTATGA TAAAGTGGGG 
CACCAGCTGA TTGTAAATGT TCTACAAGCA ACAGATCTAC CCCCTAGAGT AGATGGCCGT 
CCCAGGAATC CCTATGTAAA AATGTATTTT CTTCCAGATA GAAGCGACAA AAGTAAAAGG 
AGAACCAAAA CAGTAAAGAA ACTTCTAGAG CCAAAATGGA ACCAGACATT TGTCTACTCA 
CACGTACATC GTAGAGATTT TCGAGAGCGA ATGTTAGAGA TTACCGTGTG GGACCAGCCG 
AGAGTACAGG ACGAAGAGAG TGAATTTCTT GGAGAGATCC TCATAGAGTT GGAAACAGCG 
CTTTTAGATG ATGAGCCCCA TTGGTATAAA CTCCAGACAC ATGACGAATC TTCACTACCT 
CTGCCTCAGC CATCACCGTT CATGCCCAGG CGGCATATTC ATGGAGAGAG CTCCAGCAAA 
AAGCTACAAA GATCTCAGCG AATCAGTGAT AGTGACATCT CAGATTATGA GGTTGATGAT 
GGTATTGGAG TAGTGCCTCC AGTGGGTTAT AGAGCTAGTG CTAGAGAGAG TAAAGCAACC 
ACGTTAACAG TGCCAGAGCA ACAAAGAACT ACACATCACC GCTCACGTTC CGTGTCTCCT 
CATCGCGGCG ATGATCAGGG AAGGCCTCGT TCACGTTTAC CAAATGTGCC ATTACAGAGG 
AGCTTAGATG AAATTCATCC AACACGAAGG TCACGTTCTC CAACCCGACA CCATGATGCC 
TCCCGAAGCC CGGCCGATCA CAGATCCAGA CATGTGGAAA GTCAATATTC GTCAGAGCCA 
GACAGTGAGC TTCTCATGCT GCCCAGAGCA AAACGAGGAC GAAGTGCAGA AAGCCTACAC 
ATGACCAGTG AACTGCAGCC CTCTCTTGAC AGGGCTAGGA GTGCTAGTAC CAACTGCTTG 
AGACCAGATA CTAGTTTGCA TTCACCAGAA CGAGAAAGGG GTAGATGGTC CCCCTCCCTA 
GATAGGAGGC GACCTGCTAG CCCCAGGATT CAAATCCAGC ATGCATCTCC GGAGAATGAC 
AGGCACTCCA GAAAGTCTGA AAGATGTAGC ATCCAAAAAC AGTCTAGGAA AGGCACAGCC 
TCTGATGCAG ACAGGGTTCT CCCACCATGC CTTTCTAGAA GGGGATACGC AACCCCAAGA 
GCAACCGATC AACCGGTCGT TAGGGGAAAG CATCCCACTC GTTCACGGTC GAGCGAGCAC 
TCTAGTGTCA GAACCCTGTG TTCTATGCAC CACCTTGCCC CCGGAGGGTC GGCGCCACCT 
TCTCCACTTC TGACAAGAAC GCACCGACAA GGAAGCCCAA CCCAGTCTCC TCCAGCAGAC 
ACATCCTTCG GCAGTCGCCG TGGAAGACAG CTCCCACAGG TGCCAGTTCG AAGCGGCAGT 
ATAGAACAAG CAAGCTTAGT AGTGGAGGAG CGAACGAGAC AGATGAAAAT GAAAGTTCAC 
CGATTTAAGC AGACAACAGG GTCTGGGTCT AGTCAAGAAC TTGACCACGA GCAATACTCC 
AAGAAGTCAC GTTACATTAT GGAGTACAAC ATACATAAAG ATCAGTACAG AAGCTGTGAT 
AACGCGTCTG CCAAGTCTTC AGATAGTGAT GTCAGTGATG TGTCCGCCAT TTCCAGAGCC 
AGCAGTACCT CACGCCTCAG CAGCACAAGC TTTATGTCAG AGCAGTCTGA GCGCCCCAGG 
GGTAGGATCA GTTCATTTAC CCCCAAAATG CAAGGCAGAC GGATGGGGAC TTCAGGAAGA 
GCCATCATCA AGAGCACCAG TGTAAGTGGA GAGATATATA CACTGGAACG TAATGACGGT 
AGCCAGTCGG ACACGGCCGT GGGTACCGTC GGAGCCGGTG GAAAGAAACG AAGATCCAGC 
CTGAGCGCCA AAGTGGTAGC CATTGTGTCT CGAAGAAGCA GGAGCACGTC ACAGCTCAGC 
CAGACAGAGT CGGGCCACAA GAAGTTGAAA AGCACCATCC AGAGGAGTAC GGAAACAGGA 
ATGGCAGCTG AAATGCGGAA GATGGTGAGA CAGCCGAGCC GGGAGTCCAC GGATGGCAGC 
ATCAACAGTT ATAGCTCGGA AGGAAACTTG ATATTTCCTG GAGTTCGAGT AGGACCCGAC 
AGTCAGTTCA GTGATTTCCT TGATGGGTTG GGACCAGCGC AGCTCGTTGG CCGTCAGACG 
CTCGCCACCC CGGCCATGGG CGATATCCAA ATCGGGATGG AGGATAAGAA GGGTCAGTTG 
GAGGTTGAGG TTATCAGAGC CCGGAGCCTT ACACAAAAAC CTGGTTCCAA ATCTACACCC 
GCTCCCTATG TGAAAGTATA TCTTTTGGAA AATGGAGCCT GTATTGCCAA AAAGAAGACA 
AGAATTGCAC GGAAAACTCT CGATCCTTTG TATCAGCAGT CCCTGGTTTT TGATGAAAGT 
CCACAGGGTA AAGTTCTTCA GGTGATTGTC TGGGGTGACT ATGGAAGAAT GGACCACAAA 
TGCTTTATGG GTGTGGCTCA AATCTTGTTG GAAGAACTTG ATCTATCCAG CATGGTGATT 
GGATGGTATA AATTGTTCCC TCCGTCCTCA CTGGTGGATC CCACTCTCGC TCCCCTGACC 
CGCCGGGCTT CCCAATCATC TCTGGAAAGT TCGTCCGGGC CTCCCTGCAT CCGGTCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rims1 ( XM_017596662.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017452151.1 Rattus norvegicus regulating synaptic membrane exocytosis 1 (Rims1), transcript variant X9, mRNA.

Price & Availability↑

CloneID	ORa62879
Clone ID Related Accession (Same CDS sequence)	XM_017596662.1
Accession Version	XM_017596662.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4788bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	regulating synaptic membrane exocytosis protein 1 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005108.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCTCGG CCGTGGGGCC CCGAGGTCCT CGCCCACCCA CGGTGCCTCC CCCTATGCAA 
GAACTGCCCG ACCTGAGCCA CCTGACCGAG GAGGAGAGGA ACATTATCAT GGCAGTGATG 
GACCGGCAGA AGGAAGAGGA GGAAAAAGAA GAGGCCATGC TCAAGAGATT GCATCAACAG 
TTTGAAAGCT ACAAGGAGCA AGTGAGAAAA ATCGGAGAGG AAGCGAGGCG TTACCAGGGC 
GAGCACAAGG ATGATGCCCC GACGTGTGGA ATCTGTCATA AGACAAAGTT TGCTGATGGA 
TGTGGCCATC TCTGCTCCTA TTGTCGCACC AAGTTCTGTG CACGCTGCGG AGGCCGTGTG 
TCTCTGCGAT CGAACAATGT TATGTGGGTA TGCAATTTAT GTCGAAAGCA ACAAGAAATC 
TTAACGAAAT CTGGAGCGTG GTTCTTTGGA AGTGGCCCTC AGCAGCCTAG TCAAGATGGG 
ACTCTGAGTG ACACGGCCAC AGGTGCTGGA TCTGAGGTGC CAAGAGAAAA GAAAGCAAGG 
CTCCAAGAGC GATCAAGGTC TCAGACGCCC TTGAGTACAG CAGCTGTCTC TTCCCAAGAC 
ACTGCTACCC CCGGTGCACC GTTGGACAGG AACAAAGGGG CTGAGCCCTC ACAGCAAGCC 
TTGGGTCCTG AACAGAAGCA GGCATCAAGA TCAAGAAGCG AGCCACCGAG GGAAAGGAAG 
AAGGCTCCAG GGCTTTCAGA GCAGAATGGC AAGGGAGGCC AGAAGAGCGA GCGCAAACGT 
GTCCCCAAGT CTGTGGTGCA ACCCGGGGAA GGGATCGCGG ATGAGAGGGA GAGGAAAGAG 
AGGCGGGAAA CCCGCAGGTT GGAGAAAGGG CGCTCCCAGG ACTACTCAGA CCGGCCTGAG 
AAACGCGACA ATGGCAGGGT GGCGGAAGAC CAGAAGCAGA GGAAGGAGGA GGAGTACCAG 
ACTAGGTACC GCAGCGACCC TAACCTGGCT CGCTACCCGG TGAAGGCGCC GCCAGAGGAG 
CAGCAGATGC GCATGCACGC CCGGGTGTCC CGAGCGAGGC ACGAGCGGCG CCACAGCGAC 
GTGGCGCTCC CGCACACCGA GGCAGCTGCC GCCGCGCCGG CTGAGGCCAC GGCGGGCAAG 
CGCGCGCCGG CCACCGCCAG GGTCTCTCCC CCGGAGTCCC CGCGCGCACG CGCGCCCGCC 
GCCCAGCCTC CCACCGAGCA CGGGCCACCG CCGCCGCGGC CAGTCCCGGG TCCCGCAGAG 
CCACCCGAGC CGCGCGTCCC CGAGCCGCTC CGTAAGCAGG GCCGCCTGGA CCCGGCCTCG 
GCCGTGCTTC TGCGCAAGGC CAAGCGCGAG AAGGCGGAGA GCATGCTGCG GAACGACTCG 
CTGAGCTCCG ATCAGTCCGA GTCCGTGCGG CCATCCCCGC CCAAGCCTCA CCGGCCCAAG 
CGGGGAGGCA AGAGACGTCA GATGTCGGTG AGCAGCTCGG AGGAGGAGGG CGTGTCCACA 
CCGGAGTACA CGAGCTGCGA GGACGTGGAG CTGGAGAGCG AGAGCGTGAG CGAGAAAGGT 
GACTTGGATT ACTACTGGTT GGATCCCGCC ACGTGGCACA GCAGGGAAAC GTCGCCTATC 
AGTTCGCATC CTGTAACGTG GCAGCCGTCT AAAGAGGGAG ATCGACTAAT CGGCCGTGTT 
ATTCTTAACA AAAGAACAAC CATGCCCAAA GAATCAGGTG CATTATTGGG TCTGAAGGTG 
GTTGGAGGAA AAATGACGGA CTTAGGGCGC CTTGGTGCTT TCATCACCAA AGTAAAGAAG 
GGCAGCCTGG CAGACGTCGT CGGACACCTA AGAGCAGGGG ACGAAGTCCT AGAGTGGAAT 
GGTAAACCCC TGCCGGGAGC AACAAACGAA GAAGTTTACA ACATTATCTT AGAATCAAAA 
TCAGAACCTC AAGTTGAGAT TATTGTTTCA AGGCCTATTG GTGACATCCC CAGGATCCCT 
GAGAGTTCCC ATCCTCCCCT GGAGTCCAGT TCAAGTTCCT TTGAATCTCA GAAAATGGAA 
AGGCCTTCTA TTTCTGTTAT TTCTCCAACC AGCCCTGGAG CTCTGAAAGA TGCCCCACAA 
GTCTTACCAG GGCAACTCTC AGTGAAGCTA TGGTATGATA AAGTGGGGCA CCAGCTGATT 
GTAAATGTTC TACAAGCAAC AGATCTACCC CCTAGAGTAG ATGGCCGTCC CAGGAATCCC 
TATGTAAAAA TGTATTTTCT TCCAGATAGA AGCGACAAAA GTAAAAGGAG AACCAAAACA 
GTAAAGAAAC TTCTAGAGCC AAAATGGAAC CAGACATTTG TCTACTCACA CGTACATCGT 
AGAGATTTTC GAGAGCGAAT GTTAGAGATT ACCGTGTGGG ACCAGCCGAG AGTACAGGAC 
GAAGAGAGTG AATTTCTTGG AGAGATCCTC ATAGAGTTGG AAACAGCGCT TTTAGATGAT 
GAGCCCCATT GGTATAAACT CCAGACACAT GACGAATCTT CACTACCTCT GCCTCAGCCA 
TCACCGTTCA TGCCCAGGCG GCATATTCAT GGAGAGAGCT CCAGCAAAAA GCTACAAAGA 
TCTCAGCGAA TCAGTGATAG TGACATCTCA GATTATGAGG TTGATGATGG TATTGGAGTA 
GTGCCTCCAG TGGGTTATAG AGCTAGTGCT AGAGAGAGTA AAGCAACCAC GTTAACAGTG 
CCAGAGCAAC AAAGAACTAC ACATCACCGC TCACGTTCCG TGTCTCCTCA TCGCGGCGAT 
GATCAGGGAA GGCCTCGTTC ACGTTTACCA AATGTGCCAT TACAGAGGAG CTTAGATGAA 
ATTCATCCAA CACGAAGGTC ACGTTCTCCA ACCCGACACC ATGATGCCTC CCGAAGCCCG 
GCCGATCACA GATCCAGACA TGTGGAAAGT CAATATTCGT CAGAGCCAGA CAGTGAGCTT 
CTCATGCTGC CCAGAGCAAA ACGAGGACGA AGTGCAGAAA GCCTACACAT GACCAGTGAA 
CTGCAGCCCT CTCTTGACAG GGCTAGGAGT GCTAGTACCA ACTGCTTGAG ACCAGATACT 
AGTTTGCATT CACCAGAACG AGAAAGGGGT AGATGGTCCC CCTCCCTAGA TAGGAGGCGA 
CCTGCTAGCC CCAGGATTCA AATCCAGCAT GCATCTCCGG AGAATGACAG GCACTCCAGA 
AAGTCTGAAA GATGTAGCAT CCAAAAACAG TCTAGGAAAG GCACAGCCTC TGATGCAGAC 
AGGGTTCTCC CACCATGCCT TTCTAGAAGG GGATACGCAA CCCCAAGAGC AACCGATCAA 
CCGGTCGTTA GGGGAAAGCA TCCCACTCGT TCACGGTCGA GCGAGCACTC TAGTGTCAGA 
ACCCTGTGTT CTATGCACCA CCTTGCCCCC GGAGGGTCGG CGCCACCTTC TCCACTTCTG 
ACAAGAACGC ACCGACAAGG AAGCCCAACC CAGTCTCCTC CAGCAGACAC ATCCTTCGGC 
AGTCGCCGTG GAAGACAGCT CCCACAGGTG CCAGTTCGAA GCGGCAGTAT AGAACAAGCA 
AGCTTAGTAG TGGAGGAGCG AACGAGACAG ATGAAAATGA AAGTTCACCG ATTTAAGCAG 
ACAACAGGGT CTGGGTCTAG TCAAGAACTT GACCACGAGC AATACTCCAA GAAGTCACGT 
TACATTATGG AGTACAACAT ACATAAAGAT CAGTACAGAA GCTGTGATAA CGCGTCTGCC 
AAGTCTTCAG ATAGTGATGT CAGTGATGTG TCCGCCATTT CCAGAGCCAG CAGTACCTCA 
CGCCTCAGCA GCACAAGCTT TATGTCAGAG CAGTCTGAGC GCCCCAGGGG TAGGATCAGT 
TCATTTACCC CCAAAATGCA AGGCAGACGG ATGGGGACTT CAGGAAGAGC CATCATCAAG 
AGCACCAGTG TAAGTGGAGA GATATATACA CTGGAACGTA ATGACGGTAG CCAGTCGGAC 
ACGGCCGTGG GTACCGTCGG AGCCGGTGGA AAGAAACGAA GATCCAGCCT GAGCGCCAAA 
GTGGTAGCCA TTGTGTCTCG AAGAAGCAGG AGCACGTCAC AGCTCAGCCA GACAGAGTCG 
GGCCACAAGA AGTTGAAAAG CACCATCCAG AGGAGTACGG AAACAGGAAT GGCAGCTGAA 
ATGCGGAAGA TGGTGAGACA GCCGAGCCGG GAGTCCACGG ATGGCAGCAT CAACAGTTAT 
AGCTCGGAAG GAAACTTGAT ATTTCCTGGA GTTCGAGTAG GACCCGACAG TCAGTTCAGT 
GATTTCCTTG ATGGGTTGGG ACCAGCGCAG CTCGTTGGCC GTCAGACGCT CGCCACCCCG 
GCCATGGGCG ATATCCAAAT CGGGATGGAG GATAAGAAGG GTCAGTTGGA GGTTGAGGTT 
ATCAGAGCCC GGAGCCTTAC ACAAAAACCT GGTTCCAAAT CTACACCCGC TCCCTATGTG 
AAAGTATATC TTTTGGAAAA TGGAGCCTGT ATTGCCAAAA AGAAGACAAG AATTGCACGG 
AAAACTCTCG ATCCTTTGTA TCAGCAGTCC CTGGTTTTTG ATGAAAGTCC ACAGGGTAAA 
GTTCTTCAGG TGATTGTCTG GGGTGACTAT GGAAGAATGG ACCACAAATG CTTTATGGGT 
GTGGCTCAAA TCTTGTTGGA AGAACTTGAT CTATCCAGCA TGGTGATTGG ATGGTATAAA 
TTGTTCCCTC CGTCCTCACT GGTGGATCCC ACTCTCGCTC CCCTGACCCG CCGGGCTTCC 
CAATCATCTC TGGAAAGTTC GTCCGGGCCT CCCTGCATCC GGTCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017452151.1
CDS	313..5100
Translation	MSSAVGPRGPRPPTVPPPMQELPDLSHLTEEERNIIMAVMDRQKEEEEKEEAMLKRLHQQFESYKEQVRKIGEEARRYQGEHKDDAPTCGICHKTKFADGCGHLCSYCRTKFCARCGGRVSLRSNNVMWVCNLCRKQQEILTKSGAWFFGSGPQQPSQDGTLSDTATGAGSEVPREKKARLQERSRSQTPLSTAAVSSQDTATPGAPLDRNKGAEPSQQALGPEQKQASRSRSEPPRERKKAPGLSEQNGKGGQKSERKRVPKSVVQPGEGIADERERKERRETRRLEKGRSQDYSDRPEKRDNGRVAEDQKQRKEEEYQTRYRSDPNLARYPVKAPPEEQQMRMHARVSRARHERRHSDVALPHTEAAAAAPAEATAGKRAPATARVSPPESPRARAPAAQPPTEHGPPPPRPVPGPAEPPEPRVPEPLRKQGRLDPASAVLLRKAKREKAESMLRNDSLSSDQSESVRPSPPKPHRPKRGGKRRQMSVSSSEEEGVSTPEYTSCEDVELESESVSEKGDLDYYWLDPATWHSRETSPISSHPVTWQPSKEGDRLIGRVILNKRTTMPKESGALLGLKVVGGKMTDLGRLGAFITKVKKGSLADVVGHLRAGDEVLEWNGKPLPGATNEEVYNIILESKSEPQVEIIVSRPIGDIPRIPESSHPPLESSSSSFESQKMERPSISVISPTSPGALKDAPQVLPGQLSVKLWYDKVGHQLIVNVLQATDLPPRVDGRPRNPYVKMYFLPDRSDKSKRRTKTVKKLLEPKWNQTFVYSHVHRRDFRERMLEITVWDQPRVQDEESEFLGEILIELETALLDDEPHWYKLQTHDESSLPLPQPSPFMPRRHIHGESSSKKLQRSQRISDSDISDYEVDDGIGVVPPVGYRASARESKATTLTVPEQQRTTHHRSRSVSPHRGDDQGRPRSRLPNVPLQRSLDEIHPTRRSRSPTRHHDASRSPADHRSRHVESQYSSEPDSELLMLPRAKRGRSAESLHMTSELQPSLDRARSASTNCLRPDTSLHSPERERGRWSPSLDRRRPASPRIQIQHASPENDRHSRKSERCSIQKQSRKGTASDADRVLPPCLSRRGYATPRATDQPVVRGKHPTRSRSSEHSSVRTLCSMHHLAPGGSAPPSPLLTRTHRQGSPTQSPPADTSFGSRRGRQLPQVPVRSGSIEQASLVVEERTRQMKMKVHRFKQTTGSGSSQELDHEQYSKKSRYIMEYNIHKDQYRSCDNASAKSSDSDVSDVSAISRASSTSRLSSTSFMSEQSERPRGRISSFTPKMQGRRMGTSGRAIIKSTSVSGEIYTLERNDGSQSDTAVGTVGAGGKKRRSSLSAKVVAIVSRRSRSTSQLSQTESGHKKLKSTIQRSTETGMAAEMRKMVRQPSRESTDGSINSYSSEGNLIFPGVRVGPDSQFSDFLDGLGPAQLVGRQTLATPAMGDIQIGMEDKKGQLEVEVIRARSLTQKPGSKSTPAPYVKVYLLENGACIAKKKTRIARKTLDPLYQQSLVFDESPQGKVLQVIVWGDYGRMDHKCFMGVAQILLEELDLSSMVIGWYKLFPPSSLVDPTLAPLTRRASQSSLESSSGPPCIRS

Target ORF information:

RefSeq Version	XM_017596662.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus regulating synaptic membrane exocytosis 1 (Rims1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017596662.1

ORF Insert Sequence:

ATGTCCTCGG CCGTGGGGCC CCGAGGTCCT CGCCCACCCA CGGTGCCTCC CCCTATGCAA 
GAACTGCCCG ACCTGAGCCA CCTGACCGAG GAGGAGAGGA ACATTATCAT GGCAGTGATG 
GACCGGCAGA AGGAAGAGGA GGAAAAAGAA GAGGCCATGC TCAAGAGATT GCATCAACAG 
TTTGAAAGCT ACAAGGAGCA AGTGAGAAAA ATCGGAGAGG AAGCGAGGCG TTACCAGGGC 
GAGCACAAGG ATGATGCCCC GACGTGTGGA ATCTGTCATA AGACAAAGTT TGCTGATGGA 
TGTGGCCATC TCTGCTCCTA TTGTCGCACC AAGTTCTGTG CACGCTGCGG AGGCCGTGTG 
TCTCTGCGAT CGAACAATGT TATGTGGGTA TGCAATTTAT GTCGAAAGCA ACAAGAAATC 
TTAACGAAAT CTGGAGCGTG GTTCTTTGGA AGTGGCCCTC AGCAGCCTAG TCAAGATGGG 
ACTCTGAGTG ACACGGCCAC AGGTGCTGGA TCTGAGGTGC CAAGAGAAAA GAAAGCAAGG 
CTCCAAGAGC GATCAAGGTC TCAGACGCCC TTGAGTACAG CAGCTGTCTC TTCCCAAGAC 
ACTGCTACCC CCGGTGCACC GTTGGACAGG AACAAAGGGG CTGAGCCCTC ACAGCAAGCC 
TTGGGTCCTG AACAGAAGCA GGCATCAAGA TCAAGAAGCG AGCCACCGAG GGAAAGGAAG 
AAGGCTCCAG GGCTTTCAGA GCAGAATGGC AAGGGAGGCC AGAAGAGCGA GCGCAAACGT 
GTCCCCAAGT CTGTGGTGCA ACCCGGGGAA GGGATCGCGG ATGAGAGGGA GAGGAAAGAG 
AGGCGGGAAA CCCGCAGGTT GGAGAAAGGG CGCTCCCAGG ACTACTCAGA CCGGCCTGAG 
AAACGCGACA ATGGCAGGGT GGCGGAAGAC CAGAAGCAGA GGAAGGAGGA GGAGTACCAG 
ACTAGGTACC GCAGCGACCC TAACCTGGCT CGCTACCCGG TGAAGGCGCC GCCAGAGGAG 
CAGCAGATGC GCATGCACGC CCGGGTGTCC CGAGCGAGGC ACGAGCGGCG CCACAGCGAC 
GTGGCGCTCC CGCACACCGA GGCAGCTGCC GCCGCGCCGG CTGAGGCCAC GGCGGGCAAG 
CGCGCGCCGG CCACCGCCAG GGTCTCTCCC CCGGAGTCCC CGCGCGCACG CGCGCCCGCC 
GCCCAGCCTC CCACCGAGCA CGGGCCACCG CCGCCGCGGC CAGTCCCGGG TCCCGCAGAG 
CCACCCGAGC CGCGCGTCCC CGAGCCGCTC CGTAAGCAGG GCCGCCTGGA CCCGGCCTCG 
GCCGTGCTTC TGCGCAAGGC CAAGCGCGAG AAGGCGGAGA GCATGCTGCG GAACGACTCG 
CTGAGCTCCG ATCAGTCCGA GTCCGTGCGG CCATCCCCGC CCAAGCCTCA CCGGCCCAAG 
CGGGGAGGCA AGAGACGTCA GATGTCGGTG AGCAGCTCGG AGGAGGAGGG CGTGTCCACA 
CCGGAGTACA CGAGCTGCGA GGACGTGGAG CTGGAGAGCG AGAGCGTGAG CGAGAAAGGT 
GACTTGGATT ACTACTGGTT GGATCCCGCC ACGTGGCACA GCAGGGAAAC GTCGCCTATC 
AGTTCGCATC CTGTAACGTG GCAGCCGTCT AAAGAGGGAG ATCGACTAAT CGGCCGTGTT 
ATTCTTAACA AAAGAACAAC CATGCCCAAA GAATCAGGTG CATTATTGGG TCTGAAGGTG 
GTTGGAGGAA AAATGACGGA CTTAGGGCGC CTTGGTGCTT TCATCACCAA AGTAAAGAAG 
GGCAGCCTGG CAGACGTCGT CGGACACCTA AGAGCAGGGG ACGAAGTCCT AGAGTGGAAT 
GGTAAACCCC TGCCGGGAGC AACAAACGAA GAAGTTTACA ACATTATCTT AGAATCAAAA 
TCAGAACCTC AAGTTGAGAT TATTGTTTCA AGGCCTATTG GTGACATCCC CAGGATCCCT 
GAGAGTTCCC ATCCTCCCCT GGAGTCCAGT TCAAGTTCCT TTGAATCTCA GAAAATGGAA 
AGGCCTTCTA TTTCTGTTAT TTCTCCAACC AGCCCTGGAG CTCTGAAAGA TGCCCCACAA 
GTCTTACCAG GGCAACTCTC AGTGAAGCTA TGGTATGATA AAGTGGGGCA CCAGCTGATT 
GTAAATGTTC TACAAGCAAC AGATCTACCC CCTAGAGTAG ATGGCCGTCC CAGGAATCCC 
TATGTAAAAA TGTATTTTCT TCCAGATAGA AGCGACAAAA GTAAAAGGAG AACCAAAACA 
GTAAAGAAAC TTCTAGAGCC AAAATGGAAC CAGACATTTG TCTACTCACA CGTACATCGT 
AGAGATTTTC GAGAGCGAAT GTTAGAGATT ACCGTGTGGG ACCAGCCGAG AGTACAGGAC 
GAAGAGAGTG AATTTCTTGG AGAGATCCTC ATAGAGTTGG AAACAGCGCT TTTAGATGAT 
GAGCCCCATT GGTATAAACT CCAGACACAT GACGAATCTT CACTACCTCT GCCTCAGCCA 
TCACCGTTCA TGCCCAGGCG GCATATTCAT GGAGAGAGCT CCAGCAAAAA GCTACAAAGA 
TCTCAGCGAA TCAGTGATAG TGACATCTCA GATTATGAGG TTGATGATGG TATTGGAGTA 
GTGCCTCCAG TGGGTTATAG AGCTAGTGCT AGAGAGAGTA AAGCAACCAC GTTAACAGTG 
CCAGAGCAAC AAAGAACTAC ACATCACCGC TCACGTTCCG TGTCTCCTCA TCGCGGCGAT 
GATCAGGGAA GGCCTCGTTC ACGTTTACCA AATGTGCCAT TACAGAGGAG CTTAGATGAA 
ATTCATCCAA CACGAAGGTC ACGTTCTCCA ACCCGACACC ATGATGCCTC CCGAAGCCCG 
GCCGATCACA GATCCAGACA TGTGGAAAGT CAATATTCGT CAGAGCCAGA CAGTGAGCTT 
CTCATGCTGC CCAGAGCAAA ACGAGGACGA AGTGCAGAAA GCCTACACAT GACCAGTGAA 
CTGCAGCCCT CTCTTGACAG GGCTAGGAGT GCTAGTACCA ACTGCTTGAG ACCAGATACT 
AGTTTGCATT CACCAGAACG AGAAAGGGGT AGATGGTCCC CCTCCCTAGA TAGGAGGCGA 
CCTGCTAGCC CCAGGATTCA AATCCAGCAT GCATCTCCGG AGAATGACAG GCACTCCAGA 
AAGTCTGAAA GATGTAGCAT CCAAAAACAG TCTAGGAAAG GCACAGCCTC TGATGCAGAC 
AGGGTTCTCC CACCATGCCT TTCTAGAAGG GGATACGCAA CCCCAAGAGC AACCGATCAA 
CCGGTCGTTA GGGGAAAGCA TCCCACTCGT TCACGGTCGA GCGAGCACTC TAGTGTCAGA 
ACCCTGTGTT CTATGCACCA CCTTGCCCCC GGAGGGTCGG CGCCACCTTC TCCACTTCTG 
ACAAGAACGC ACCGACAAGG AAGCCCAACC CAGTCTCCTC CAGCAGACAC ATCCTTCGGC 
AGTCGCCGTG GAAGACAGCT CCCACAGGTG CCAGTTCGAA GCGGCAGTAT AGAACAAGCA 
AGCTTAGTAG TGGAGGAGCG AACGAGACAG ATGAAAATGA AAGTTCACCG ATTTAAGCAG 
ACAACAGGGT CTGGGTCTAG TCAAGAACTT GACCACGAGC AATACTCCAA GAAGTCACGT 
TACATTATGG AGTACAACAT ACATAAAGAT CAGTACAGAA GCTGTGATAA CGCGTCTGCC 
AAGTCTTCAG ATAGTGATGT CAGTGATGTG TCCGCCATTT CCAGAGCCAG CAGTACCTCA 
CGCCTCAGCA GCACAAGCTT TATGTCAGAG CAGTCTGAGC GCCCCAGGGG TAGGATCAGT 
TCATTTACCC CCAAAATGCA AGGCAGACGG ATGGGGACTT CAGGAAGAGC CATCATCAAG 
AGCACCAGTG TAAGTGGAGA GATATATACA CTGGAACGTA ATGACGGTAG CCAGTCGGAC 
ACGGCCGTGG GTACCGTCGG AGCCGGTGGA AAGAAACGAA GATCCAGCCT GAGCGCCAAA 
GTGGTAGCCA TTGTGTCTCG AAGAAGCAGG AGCACGTCAC AGCTCAGCCA GACAGAGTCG 
GGCCACAAGA AGTTGAAAAG CACCATCCAG AGGAGTACGG AAACAGGAAT GGCAGCTGAA 
ATGCGGAAGA TGGTGAGACA GCCGAGCCGG GAGTCCACGG ATGGCAGCAT CAACAGTTAT 
AGCTCGGAAG GAAACTTGAT ATTTCCTGGA GTTCGAGTAG GACCCGACAG TCAGTTCAGT 
GATTTCCTTG ATGGGTTGGG ACCAGCGCAG CTCGTTGGCC GTCAGACGCT CGCCACCCCG 
GCCATGGGCG ATATCCAAAT CGGGATGGAG GATAAGAAGG GTCAGTTGGA GGTTGAGGTT 
ATCAGAGCCC GGAGCCTTAC ACAAAAACCT GGTTCCAAAT CTACACCCGC TCCCTATGTG 
AAAGTATATC TTTTGGAAAA TGGAGCCTGT ATTGCCAAAA AGAAGACAAG AATTGCACGG 
AAAACTCTCG ATCCTTTGTA TCAGCAGTCC CTGGTTTTTG ATGAAAGTCC ACAGGGTAAA 
GTTCTTCAGG TGATTGTCTG GGGTGACTAT GGAAGAATGG ACCACAAATG CTTTATGGGT 
GTGGCTCAAA TCTTGTTGGA AGAACTTGAT CTATCCAGCA TGGTGATTGG ATGGTATAAA 
TTGTTCCCTC CGTCCTCACT GGTGGATCCC ACTCTCGCTC CCCTGACCCG CCGGGCTTCC 
CAATCATCTC TGGAAAGTTC GTCCGGGCCT CCCTGCATCC GGTCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rims1 ( XM_017596663.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017452152.1 Rattus norvegicus regulating synaptic membrane exocytosis 1 (Rims1), transcript variant X10, mRNA.

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CloneID	ORa62880
Clone ID Related Accession (Same CDS sequence)	XM_017596663.1
Accession Version	XM_017596663.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4767bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	regulating synaptic membrane exocytosis protein 1 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005108.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCTCGG CCGTGGGGCC CCGAGGTCCT CGCCCACCCA CGGTGCCTCC CCCTATGCAA 
GAACTGCCCG ACCTGAGCCA CCTGACCGAG GAGGAGAGGA ACATTATCAT GGCAGTGATG 
GACCGGCAGA AGGAAGAGGA GGAAAAAGAA GAGGCCATGC TCAAGTGTGT TGTCAGGGAC 
ATGGCGAAGC CTGCTGCCTG CAAAACACCA AGAAATGCTG AAAGCCAGCC CCATCAACCA 
CCACTGAACA TTTTCAGATG TGTCTGTGTT CCCAGAAAGC CAAGCAGCGA AGAGGGAGGC 
CCAGAAAGAG ACTGGAGATT GCATCAACAG TTTGAAAGCT ACAAGGAGCA AGTGAGAAAA 
ATCGGAGAGG AAGCGAGGCG TTACCAGGGC GAGCACAAGG ATGATGCCCC GACGTGTGGA 
ATCTGTCATA AGACAAAGTT TGCTGATGGA TGTGGCCATC TCTGCTCCTA TTGTCGCACC 
AAGTTCTGTG CACGCTGCGG AGGCCGTGTG TCTCTGCGAT CGAACAATGA GGACAAAGTG 
GTTATGTGGG TATGCAATTT ATGTCGAAAG CAACAAGAAA TCTTAACGAA ATCTGGAGCG 
TGGTTCTTTG GAAGTGGCCC TCAGCAGCCT AGTCAAGATG GGACTCTGAG TGACACGGCC 
ACAGGTGCTG GATCTGAGGT GCCAAGAGAA AAGAAAGCAA GGCTCCAAGA GCGATCAAGG 
TCTCAGACGC CCTTGAGTAC AGCAGCTGTC TCTTCCCAAG ACACTGCTAC CCCCGGTGCA 
CCGTTGGACA GGAACAAAGG GGCTGAGCCC TCACAGCAAG CCTTGGGTCC TGAACAGAAG 
CAGGCATCAA GATCAAGAAG CGAGCCACCG AGGGAAAGGA AGAAGGCTCC AGGGCTTTCA 
GAGCAGAATG GCAAGGGAGG CCAGAAGAGC GAGCGCAAAC GTGTCCCCAA GTCTGTGGTG 
CAACCCGGGG AAGGGATCGC GGATGAGAGG GAGAGGAAAG AGAGGCGGGA AACCCGCAGG 
TTGGAGAAAG GGCGCTCCCA GGACTACTCA GACCGGCCTG AGAAACGCGA CAATGGCAGG 
GTGGCGGAAG ACCAGAAGCA GAGGAAGGAG GAGGAGTACC AGACTAGGTA CCGCAGCGAC 
CCTAACCTGG CTCGCTACCC GGTGAAGGCG CCGCCAGAGG AGCAGCAGAT GCGCATGCAC 
GCCCGGGTGT CCCGAGCGAG GCACGAGCGG CGCCACAGCG ACGTGGCGCT CCCGCACACC 
GAGGCAGCTG CCGCCGCGCC GGCTGAGGCC ACGGCGGGCA AGCGCGCGCC GGCCACCGCC 
AGGGTCTCTC CCCCGGAGTC CCCGCGCGCA CGCGCGCCCG CCGCCCAGCC TCCCACCGAG 
CACGGGCCAC CGCCGCCGCG GCCAGTCCCG GGTCCCGCAG AGCCACCCGA GCCGCGCGTC 
CCCGAGCCGC TCCGTAAGCA GGGCCGCCTG GACCCGGCCT CGGCCGTGCT TCTGCGCAAG 
GCCAAGCGCG AGAAGGCGGA GAGCATGCTG CGGAACGACT CGCTGAGCTC CGATCAGTCC 
GAGTCCGTGC GGCCATCCCC GCCCAAGCCT CACCGGCCCA AGCGGGGAGG CAAGAGACGT 
CAGATGTCGG TGAGCAGCTC GGAGGAGGAG GGCGTGTCCA CACCGGAGTA CACGAGCTGC 
GAGGACGTGG AGCTGGAGAG CGAGAGCGTG AGCGAGAAAG GTGACTTGGA TTACTACTGG 
TTGGATCCCG CCACGTGGCA CAGCAGGGAA ACGTCGCCTA TCAGTTCGCA TCCTGTAACG 
TGGCAGCCGT CTAAAGAGGG AGATCGACTA ATCGGCCGTG TTATTCTTAA CAAAAGAACA 
ACCATGCCCA AAGAATCAGG TGCATTATTG GGTCTGAAGG TGGTTGGAGG AAAAATGACG 
GACTTAGGGC GCCTTGGTGC TTTCATCACC AAAGTAAAGA AGGGCAGCCT GGCAGACGTC 
GTCGGACACC TAAGAGCAGG GGACGAAGTC CTAGAGTGGA ATGGTAAACC CCTGCCGGGA 
GCAACAAACG AAGAAGTTTA CAACATTATC TTAGAATCAA AATCAGAACC TCAAGTTGAG 
ATTATTGTTT CAAGGCCTAT TGGTGACATC CCCAGGATCC CTGAGAGTTC CCATCCTCCC 
CTGGAGTCCA GTTCAAGTTC CTTTGAATCT CAGAAAATGG AAAGGCCTTC TATTTCTGTT 
ATTTCTCCAA CCAGCCCTGG AGCTCTGAAA GATGCCCCAC AAGTCTTACC AGGGCAACTC 
TCAGTGAAGC TATGGTATGA TAAAGTGGGG CACCAGCTGA TTGTAAATGT TCTACAAGCA 
ACAGATCTAC CCCCTAGAGT AGATGGCCGT CCCAGGAATC CCTATGTAAA AATGTATTTT 
CTTCCAGATA GAAGCGACAA AAGTAAAAGG AGAACCAAAA CAGTAAAGAA ACTTCTAGAG 
CCAAAATGGA ACCAGACATT TGTCTACTCA CACGTACATC GTAGAGATTT TCGAGAGCGA 
ATGTTAGAGA TTACCGTGTG GGACCAGCCG AGAGTACAGG ACGAAGAGAG TGAATTTCTT 
GGAGAGATCC TCATAGAGTT GGAAACAGCG CTTTTAGATG ATGAGCCCCA TTGGTATAAA 
CTCCAGACAC ATGACGAATC TTCACTACCT CTGCCTCAGC CATCACCGTT CATGCCCAGG 
CGGCATATTC ATGGAGAGAG CTCCAGCAAA AAGCTACAAA GATCTCAGCG AATCAGTGAT 
AGTGACATCT CAGATTATGA GGTTGATGAT GGTATTGGAG TAGTGCCTCC AGTGGGTTAT 
AGAGCTAGTG CTAGAGAGAG TAAAGCAACC ACGTTAACAG TGCCAGAGCA ACAAAGAACT 
ACACATCACC GCTCACGTTC CGTGTCTCCT CATCGCGGCG ATGATCAGGG AAGGCCTCGT 
TCACGTTTAC CAAATGTGCC ATTACAGAGG AGCTTAGATG AAATTCATCC AACACGAAGG 
TCACGTTCTC CAACCCGACA CCATGATGCC TCCCGAAGCC CGGCCGATCA CAGATCCAGA 
CATGTGGAAA GTCAATATTC GTCAGAGCCA GACAGTGAGC TTCTCATGCT GCCCAGAGCA 
AAACGAGGAC GAAGTGCAGA AAGCCTACAC ATGACCAGTG AACTGCAGCC CTCTCTTGAC 
AGGGCTAGGA GTGCTAGTAC CAACTGCTTG AGACCAGATA CTAGTTTGCA TTCACCAGAA 
CGAGAAAGGG GTAGATGGTC CCCCTCCCTA GATAGGAGGC GACCTGCTAG CCCCAGGATT 
CAAATCCAGC ATGCATCTCC GGAGAATGAC AGGCACTCCA GAAAGTCTGA AAGATGTAGC 
ATCCAAAAAC AGTCTAGGAA AGGCACAGCC TCTGATGCAG ACAGAACGCA CCGACAAGGA 
AGCCCAACCC AGTCTCCTCC AGCAGACACA TCCTTCGGCA GTCGCCGTGG AAGACAGCTC 
CCACAGGTGC CAGTTCGAAG CGGCAGTATA GAACAAGCAA GCTTAGTAGT GGAGGAGCGA 
ACGAGACAGA TGAAAATGAA AGTTCACCGA TTTAAGCAGA CAACAGGGTC TGGGTCTAGT 
CAAGAACTTG ACCACGAGCA ATACTCCAAG AAGTCACGTT ACATTATGGA GTACAACATA 
CATAAAGATC AGTACAGAAG CTGTGATAAC GCGTCTGCCA AGTCTTCAGA TAGTGATGTC 
AGTGATGTGT CCGCCATTTC CAGAGCCAGC AGTACCTCAC GCCTCAGCAG CACAAGCTTT 
ATGTCAGAGC AGTCTGAGCG CCCCAGGGGT AGGATCAGTT CATTTACCCC CAAAATGCAA 
GGCAGACGGA TGGGGACTTC AGGAAGAGCC ATCATCAAGA GCACCAGTGT AAGTGGAGAG 
ATATATACAC TGGAACGTAA TGACGGTAGC CAGTCGGACA CGGCCGTGGG TACCGTCGGA 
GCCGGTGGAA AGAAACGAAG ATCCAGCCTG AGCGCCAAAG TGGTAGCCAT TGTGTCTCGA 
AGAAGCAGGA GCACGTCACA GCTCAGCCAG ACAGAGTCGG GCCACAAGAA GTTGAAAAGC 
ACCATCCAGA GGAGTACGGA AACAGGAATG GCAGCTGAAA TGCGGAAGAT GGTGAGACAG 
CCGAGCCGGG AGTCCACGGA TGGCAGCATC AACAGTTATA GCTCGGAAGG AAACTTGATA 
TTTCCTGGAG TTCGAGTAGG ACCCGACAGT CAGTTCAGTG ATTTCCTTGA TGGGTTGGGA 
CCAGCGCAGC TCGTTGGCCG TCAGACGCTC GCCACCCCGG CCATGGGCGA TATCCAAATC 
GGGATGGAGG ATAAGAAGGG TCAGTTGGAG GTTGAGGTTA TCAGAGCCCG GAGCCTTACA 
CAAAAACCTG GTTCCAAATC TACACCCGCT CCCTATGTGA AAGTATATCT TTTGGAAAAT 
GGAGCCTGTA TTGCCAAAAA GAAGACAAGA ATTGCACGGA AAACTCTCGA TCCTTTGTAT 
CAGCAGTCCC TGGTTTTTGA TGAAAGTCCA CAGGGTAAAG TTCTTCAGGT GATTGTCTGG 
GGTGACTATG GAAGAATGGA CCACAAATGC TTTATGGGTG TGGCTCAAAT CTTGTTGGAA 
GAACTTGATC TATCCAGCAT GGTGATTGGA TGGTATAAAT TGTTCCCTCC GTCCTCACTG 
GTGGATCCCA CTCTCGCTCC CCTGACCCGC CGGGCTTCCC AATCATCTCT GGAAAGTTCG 
TCCGGGCCTC CCTGCATCCG GTCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017452152.1
CDS	310..5076
Translation	MSSAVGPRGPRPPTVPPPMQELPDLSHLTEEERNIIMAVMDRQKEEEEKEEAMLKCVVRDMAKPAACKTPRNAESQPHQPPLNIFRCVCVPRKPSSEEGGPERDWRLHQQFESYKEQVRKIGEEARRYQGEHKDDAPTCGICHKTKFADGCGHLCSYCRTKFCARCGGRVSLRSNNEDKVVMWVCNLCRKQQEILTKSGAWFFGSGPQQPSQDGTLSDTATGAGSEVPREKKARLQERSRSQTPLSTAAVSSQDTATPGAPLDRNKGAEPSQQALGPEQKQASRSRSEPPRERKKAPGLSEQNGKGGQKSERKRVPKSVVQPGEGIADERERKERRETRRLEKGRSQDYSDRPEKRDNGRVAEDQKQRKEEEYQTRYRSDPNLARYPVKAPPEEQQMRMHARVSRARHERRHSDVALPHTEAAAAAPAEATAGKRAPATARVSPPESPRARAPAAQPPTEHGPPPPRPVPGPAEPPEPRVPEPLRKQGRLDPASAVLLRKAKREKAESMLRNDSLSSDQSESVRPSPPKPHRPKRGGKRRQMSVSSSEEEGVSTPEYTSCEDVELESESVSEKGDLDYYWLDPATWHSRETSPISSHPVTWQPSKEGDRLIGRVILNKRTTMPKESGALLGLKVVGGKMTDLGRLGAFITKVKKGSLADVVGHLRAGDEVLEWNGKPLPGATNEEVYNIILESKSEPQVEIIVSRPIGDIPRIPESSHPPLESSSSSFESQKMERPSISVISPTSPGALKDAPQVLPGQLSVKLWYDKVGHQLIVNVLQATDLPPRVDGRPRNPYVKMYFLPDRSDKSKRRTKTVKKLLEPKWNQTFVYSHVHRRDFRERMLEITVWDQPRVQDEESEFLGEILIELETALLDDEPHWYKLQTHDESSLPLPQPSPFMPRRHIHGESSSKKLQRSQRISDSDISDYEVDDGIGVVPPVGYRASARESKATTLTVPEQQRTTHHRSRSVSPHRGDDQGRPRSRLPNVPLQRSLDEIHPTRRSRSPTRHHDASRSPADHRSRHVESQYSSEPDSELLMLPRAKRGRSAESLHMTSELQPSLDRARSASTNCLRPDTSLHSPERERGRWSPSLDRRRPASPRIQIQHASPENDRHSRKSERCSIQKQSRKGTASDADRTHRQGSPTQSPPADTSFGSRRGRQLPQVPVRSGSIEQASLVVEERTRQMKMKVHRFKQTTGSGSSQELDHEQYSKKSRYIMEYNIHKDQYRSCDNASAKSSDSDVSDVSAISRASSTSRLSSTSFMSEQSERPRGRISSFTPKMQGRRMGTSGRAIIKSTSVSGEIYTLERNDGSQSDTAVGTVGAGGKKRRSSLSAKVVAIVSRRSRSTSQLSQTESGHKKLKSTIQRSTETGMAAEMRKMVRQPSRESTDGSINSYSSEGNLIFPGVRVGPDSQFSDFLDGLGPAQLVGRQTLATPAMGDIQIGMEDKKGQLEVEVIRARSLTQKPGSKSTPAPYVKVYLLENGACIAKKKTRIARKTLDPLYQQSLVFDESPQGKVLQVIVWGDYGRMDHKCFMGVAQILLEELDLSSMVIGWYKLFPPSSLVDPTLAPLTRRASQSSLESSSGPPCIRS

Target ORF information:

RefSeq Version	XM_017596663.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus regulating synaptic membrane exocytosis 1 (Rims1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017596663.1

ORF Insert Sequence:

ATGTCCTCGG CCGTGGGGCC CCGAGGTCCT CGCCCACCCA CGGTGCCTCC CCCTATGCAA 
GAACTGCCCG ACCTGAGCCA CCTGACCGAG GAGGAGAGGA ACATTATCAT GGCAGTGATG 
GACCGGCAGA AGGAAGAGGA GGAAAAAGAA GAGGCCATGC TCAAGTGTGT TGTCAGGGAC 
ATGGCGAAGC CTGCTGCCTG CAAAACACCA AGAAATGCTG AAAGCCAGCC CCATCAACCA 
CCACTGAACA TTTTCAGATG TGTCTGTGTT CCCAGAAAGC CAAGCAGCGA AGAGGGAGGC 
CCAGAAAGAG ACTGGAGATT GCATCAACAG TTTGAAAGCT ACAAGGAGCA AGTGAGAAAA 
ATCGGAGAGG AAGCGAGGCG TTACCAGGGC GAGCACAAGG ATGATGCCCC GACGTGTGGA 
ATCTGTCATA AGACAAAGTT TGCTGATGGA TGTGGCCATC TCTGCTCCTA TTGTCGCACC 
AAGTTCTGTG CACGCTGCGG AGGCCGTGTG TCTCTGCGAT CGAACAATGA GGACAAAGTG 
GTTATGTGGG TATGCAATTT ATGTCGAAAG CAACAAGAAA TCTTAACGAA ATCTGGAGCG 
TGGTTCTTTG GAAGTGGCCC TCAGCAGCCT AGTCAAGATG GGACTCTGAG TGACACGGCC 
ACAGGTGCTG GATCTGAGGT GCCAAGAGAA AAGAAAGCAA GGCTCCAAGA GCGATCAAGG 
TCTCAGACGC CCTTGAGTAC AGCAGCTGTC TCTTCCCAAG ACACTGCTAC CCCCGGTGCA 
CCGTTGGACA GGAACAAAGG GGCTGAGCCC TCACAGCAAG CCTTGGGTCC TGAACAGAAG 
CAGGCATCAA GATCAAGAAG CGAGCCACCG AGGGAAAGGA AGAAGGCTCC AGGGCTTTCA 
GAGCAGAATG GCAAGGGAGG CCAGAAGAGC GAGCGCAAAC GTGTCCCCAA GTCTGTGGTG 
CAACCCGGGG AAGGGATCGC GGATGAGAGG GAGAGGAAAG AGAGGCGGGA AACCCGCAGG 
TTGGAGAAAG GGCGCTCCCA GGACTACTCA GACCGGCCTG AGAAACGCGA CAATGGCAGG 
GTGGCGGAAG ACCAGAAGCA GAGGAAGGAG GAGGAGTACC AGACTAGGTA CCGCAGCGAC 
CCTAACCTGG CTCGCTACCC GGTGAAGGCG CCGCCAGAGG AGCAGCAGAT GCGCATGCAC 
GCCCGGGTGT CCCGAGCGAG GCACGAGCGG CGCCACAGCG ACGTGGCGCT CCCGCACACC 
GAGGCAGCTG CCGCCGCGCC GGCTGAGGCC ACGGCGGGCA AGCGCGCGCC GGCCACCGCC 
AGGGTCTCTC CCCCGGAGTC CCCGCGCGCA CGCGCGCCCG CCGCCCAGCC TCCCACCGAG 
CACGGGCCAC CGCCGCCGCG GCCAGTCCCG GGTCCCGCAG AGCCACCCGA GCCGCGCGTC 
CCCGAGCCGC TCCGTAAGCA GGGCCGCCTG GACCCGGCCT CGGCCGTGCT TCTGCGCAAG 
GCCAAGCGCG AGAAGGCGGA GAGCATGCTG CGGAACGACT CGCTGAGCTC CGATCAGTCC 
GAGTCCGTGC GGCCATCCCC GCCCAAGCCT CACCGGCCCA AGCGGGGAGG CAAGAGACGT 
CAGATGTCGG TGAGCAGCTC GGAGGAGGAG GGCGTGTCCA CACCGGAGTA CACGAGCTGC 
GAGGACGTGG AGCTGGAGAG CGAGAGCGTG AGCGAGAAAG GTGACTTGGA TTACTACTGG 
TTGGATCCCG CCACGTGGCA CAGCAGGGAA ACGTCGCCTA TCAGTTCGCA TCCTGTAACG 
TGGCAGCCGT CTAAAGAGGG AGATCGACTA ATCGGCCGTG TTATTCTTAA CAAAAGAACA 
ACCATGCCCA AAGAATCAGG TGCATTATTG GGTCTGAAGG TGGTTGGAGG AAAAATGACG 
GACTTAGGGC GCCTTGGTGC TTTCATCACC AAAGTAAAGA AGGGCAGCCT GGCAGACGTC 
GTCGGACACC TAAGAGCAGG GGACGAAGTC CTAGAGTGGA ATGGTAAACC CCTGCCGGGA 
GCAACAAACG AAGAAGTTTA CAACATTATC TTAGAATCAA AATCAGAACC TCAAGTTGAG 
ATTATTGTTT CAAGGCCTAT TGGTGACATC CCCAGGATCC CTGAGAGTTC CCATCCTCCC 
CTGGAGTCCA GTTCAAGTTC CTTTGAATCT CAGAAAATGG AAAGGCCTTC TATTTCTGTT 
ATTTCTCCAA CCAGCCCTGG AGCTCTGAAA GATGCCCCAC AAGTCTTACC AGGGCAACTC 
TCAGTGAAGC TATGGTATGA TAAAGTGGGG CACCAGCTGA TTGTAAATGT TCTACAAGCA 
ACAGATCTAC CCCCTAGAGT AGATGGCCGT CCCAGGAATC CCTATGTAAA AATGTATTTT 
CTTCCAGATA GAAGCGACAA AAGTAAAAGG AGAACCAAAA CAGTAAAGAA ACTTCTAGAG 
CCAAAATGGA ACCAGACATT TGTCTACTCA CACGTACATC GTAGAGATTT TCGAGAGCGA 
ATGTTAGAGA TTACCGTGTG GGACCAGCCG AGAGTACAGG ACGAAGAGAG TGAATTTCTT 
GGAGAGATCC TCATAGAGTT GGAAACAGCG CTTTTAGATG ATGAGCCCCA TTGGTATAAA 
CTCCAGACAC ATGACGAATC TTCACTACCT CTGCCTCAGC CATCACCGTT CATGCCCAGG 
CGGCATATTC ATGGAGAGAG CTCCAGCAAA AAGCTACAAA GATCTCAGCG AATCAGTGAT 
AGTGACATCT CAGATTATGA GGTTGATGAT GGTATTGGAG TAGTGCCTCC AGTGGGTTAT 
AGAGCTAGTG CTAGAGAGAG TAAAGCAACC ACGTTAACAG TGCCAGAGCA ACAAAGAACT 
ACACATCACC GCTCACGTTC CGTGTCTCCT CATCGCGGCG ATGATCAGGG AAGGCCTCGT 
TCACGTTTAC CAAATGTGCC ATTACAGAGG AGCTTAGATG AAATTCATCC AACACGAAGG 
TCACGTTCTC CAACCCGACA CCATGATGCC TCCCGAAGCC CGGCCGATCA CAGATCCAGA 
CATGTGGAAA GTCAATATTC GTCAGAGCCA GACAGTGAGC TTCTCATGCT GCCCAGAGCA 
AAACGAGGAC GAAGTGCAGA AAGCCTACAC ATGACCAGTG AACTGCAGCC CTCTCTTGAC 
AGGGCTAGGA GTGCTAGTAC CAACTGCTTG AGACCAGATA CTAGTTTGCA TTCACCAGAA 
CGAGAAAGGG GTAGATGGTC CCCCTCCCTA GATAGGAGGC GACCTGCTAG CCCCAGGATT 
CAAATCCAGC ATGCATCTCC GGAGAATGAC AGGCACTCCA GAAAGTCTGA AAGATGTAGC 
ATCCAAAAAC AGTCTAGGAA AGGCACAGCC TCTGATGCAG ACAGAACGCA CCGACAAGGA 
AGCCCAACCC AGTCTCCTCC AGCAGACACA TCCTTCGGCA GTCGCCGTGG AAGACAGCTC 
CCACAGGTGC CAGTTCGAAG CGGCAGTATA GAACAAGCAA GCTTAGTAGT GGAGGAGCGA 
ACGAGACAGA TGAAAATGAA AGTTCACCGA TTTAAGCAGA CAACAGGGTC TGGGTCTAGT 
CAAGAACTTG ACCACGAGCA ATACTCCAAG AAGTCACGTT ACATTATGGA GTACAACATA 
CATAAAGATC AGTACAGAAG CTGTGATAAC GCGTCTGCCA AGTCTTCAGA TAGTGATGTC 
AGTGATGTGT CCGCCATTTC CAGAGCCAGC AGTACCTCAC GCCTCAGCAG CACAAGCTTT 
ATGTCAGAGC AGTCTGAGCG CCCCAGGGGT AGGATCAGTT CATTTACCCC CAAAATGCAA 
GGCAGACGGA TGGGGACTTC AGGAAGAGCC ATCATCAAGA GCACCAGTGT AAGTGGAGAG 
ATATATACAC TGGAACGTAA TGACGGTAGC CAGTCGGACA CGGCCGTGGG TACCGTCGGA 
GCCGGTGGAA AGAAACGAAG ATCCAGCCTG AGCGCCAAAG TGGTAGCCAT TGTGTCTCGA 
AGAAGCAGGA GCACGTCACA GCTCAGCCAG ACAGAGTCGG GCCACAAGAA GTTGAAAAGC 
ACCATCCAGA GGAGTACGGA AACAGGAATG GCAGCTGAAA TGCGGAAGAT GGTGAGACAG 
CCGAGCCGGG AGTCCACGGA TGGCAGCATC AACAGTTATA GCTCGGAAGG AAACTTGATA 
TTTCCTGGAG TTCGAGTAGG ACCCGACAGT CAGTTCAGTG ATTTCCTTGA TGGGTTGGGA 
CCAGCGCAGC TCGTTGGCCG TCAGACGCTC GCCACCCCGG CCATGGGCGA TATCCAAATC 
GGGATGGAGG ATAAGAAGGG TCAGTTGGAG GTTGAGGTTA TCAGAGCCCG GAGCCTTACA 
CAAAAACCTG GTTCCAAATC TACACCCGCT CCCTATGTGA AAGTATATCT TTTGGAAAAT 
GGAGCCTGTA TTGCCAAAAA GAAGACAAGA ATTGCACGGA AAACTCTCGA TCCTTTGTAT 
CAGCAGTCCC TGGTTTTTGA TGAAAGTCCA CAGGGTAAAG TTCTTCAGGT GATTGTCTGG 
GGTGACTATG GAAGAATGGA CCACAAATGC TTTATGGGTG TGGCTCAAAT CTTGTTGGAA 
GAACTTGATC TATCCAGCAT GGTGATTGGA TGGTATAAAT TGTTCCCTCC GTCCTCACTG 
GTGGATCCCA CTCTCGCTCC CCTGACCCGC CGGGCTTCCC AATCATCTCT GGAAAGTTCG 
TCCGGGCCTC CCTGCATCCG GTCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rims1 ( XM_017596664.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017452153.1 Rattus norvegicus regulating synaptic membrane exocytosis 1 (Rims1), transcript variant X11, mRNA.

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CloneID	ORa62881
Clone ID Related Accession (Same CDS sequence)	XM_017596664.1
Accession Version	XM_017596664.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4695bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	regulating synaptic membrane exocytosis protein 1 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005108.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCTCGG CCGTGGGGCC CCGAGGTCCT CGCCCACCCA CGGTGCCTCC CCCTATGCAA 
GAACTGCCCG ACCTGAGCCA CCTGACCGAG GAGGAGAGGA ACATTATCAT GGCAGTGATG 
GACCGGCAGA AGGAAGAGGA GGAAAAAGAA GAGGCCATGC TCAAGTGTGT TGTCAGGGAC 
ATGGCGAAGC CTGCTGCCTG CAAAACACCA AGAAATGCTG AAAGCCAGCC CCATCAACCA 
CCACTGAACA TTTTCAGATG TGTCTGTGTT CCCAGAAAGC CAAGCAGCGA AGAGGGAGGC 
CCAGAAAGAG ACTGGAGATT GCATCAACAG TTTGAAAGCT ACAAGGAGCA AGTGAGAAAA 
ATCGGAGAGG AAGCGAGGCG TTACCAGGGC GAGCACAAGG ATGATGCCCC GACGTGTGGA 
ATCTGTCATA AGACAAAGTT TGCTGATGGA TGTGGCCATC TCTGCTCCTA TTGTCGCACC 
AAGTTCTGTG CACGCTGCGG AGGCCGTGTG TCTCTGCGAT CGAACAATGA GGACAAAGTG 
GTTATGTGGG TATGCAATTT ATGTCGAAAG CAACAAGAAA TCTTAACGAA ATCTGGAGCG 
TGGTTCTTTG GAAGTGGCCC TCAGCAGCCT AGTCAAGATG GGACTCTGAG TGACACGGCC 
ACAGGTGCTG GATCTGAGGT GCCAAGAGAA AAGAAAGCAA GGCTCCAAGA GCGATCAAGG 
TCTCAGACGC CCTTGAGTAC AGCAGCTGTC TCTTCCCAAG ACACTGCTAC CCCCGGTGCA 
CCGTTGGACA GGAACAAAGG GGCTGAGCCC TCACAGCAAG CCTTGGGTCC TGAACAGAAG 
CAGGCATCAA GATCAAGAAG CGAGCCACCG AGGGAAAGGA AGAAGGCTCC AGGGCTTTCA 
GAGCAGAATG GCAAGGGAGG CCAGAAGAGC GAGCGCAAAC GTGTCCCCAA GTCTGTGGTG 
CAACCCGGGG AAGGGATCGC GGATGAGAGG GAGAGGAAAG AGAGGCGGGA AACCCGCAGG 
TTGGAGAAAG GGCGCTCCCA GGACTACTCA GACCGGCCTG AGAAACGCGA CAATGGCAGG 
GTGGCGGAAG ACCAGAAGCA GAGGAAGGAG GAGGAGTACC AGACTAGGTA CCGCAGCGAC 
CCTAACCTGG CTCGCTACCC GGTGAAGGCG CCGCCAGAGG AGCAGCAGAT GCGCATGCAC 
GCCCGGGTGT CCCGAGCGAG GCACGAGCGG CGCCACAGCG ACGTGGCGCT CCCGCACACC 
GAGGCAGCTG CCGCCGCGCC GGCTGAGGCC ACGGCGGGCA AGCGCGCGCC GGCCACCGCC 
AGGGTCTCTC CCCCGGAGTC CCCGCGCGCA CGCGCGCCCG CCGCCCAGCC TCCCACCGAG 
CACGGGCCAC CGCCGCCGCG GCCAGTCCCG GGTCCCGCAG AGCCACCCGA GCCGCGCGTC 
CCCGAGCCGC TCCGTAAGCA GGGCCGCCTG GACCCGGCCT CGGCCGTGCT TCTGCGCAAG 
GCCAAGCGCG AGAAGGCGGA GAGCATGCTG CGGAACGACT CGCTGAGCTC CGATCAGTCC 
GAGTCCGTGC GGCCATCCCC GCCCAAGCCT CACCGGCCCA AGCGGGGAGG CAAGAGACGT 
CAGATGTCGG TGAGCAGCTC GGAGGAGGAG GGCGTGTCCA CACCGGAGTA CACGAGCTGC 
GAGGACGTGG AGCTGGAGAG CGAGAGCGTG AGCGAGAAAG GTGACTTGGA TTACTACTGG 
TTGGATCCCG CCACGTGGCA CAGCAGGGAA ACGTCGCCTA TCAGTTCGCA TCCTGTAACG 
TGGCAGCCGT CTAAAGAGGG AGATCGACTA ATCGGCCGTG TTATTCTTAA CAAAAGAACA 
ACCATGCCCA AAGAATCAGG TGCATTATTG GGTCTGAAGG TGGTTGGAGG AAAAATGACG 
GACTTAGGGC GCCTTGGTGC TTTCATCACC AAAGTAAAGA AGGGCAGCCT GGCAGACGTC 
GTCGGACACC TAAGAGCAGG GGACGAAGTC CTAGAGTGGA ATGGTAAACC CCTGCCGGGA 
GCAACAAACG AAGAAGTTTA CAACATTATC TTAGAATCAA AATCAGAACC TCAAGTTGAG 
ATTATTGTTT CAAGGCCTAT TGGTGACATC CCCAGGATCC CTGAGAGTTC CCATCCTCCC 
CTGGAGTCCA GTTCAAGTTC CTTTGAATCT CAGAAAATGG AAAGGCCTTC TATTTCTGTT 
ATTTCTCCAA CCAGCCCTGG AGCTCTGAAA GATGCCCCAC AAGTCTTACC AGGGCAACTC 
TCAGTGAAGC TATGGTATGA TAAAGTGGGG CACCAGCTGA TTGTAAATGT TCTACAAGCA 
ACAGATCTAC CCCCTAGAGT AGATGGCCGT CCCAGGAATC CCTATGTAAA AATGTATTTT 
CTTCCAGATA GAAGCGACAA AAGTAAAAGG AGAACCAAAA CAGTAAAGAA ACTTCTAGAG 
CCAAAATGGA ACCAGACATT TGTCTACTCA CACGTACATC GTAGAGATTT TCGAGAGCGA 
ATGTTAGAGA TTACCGTGTG GGACCAGCCG AGAGTACAGG ACGAAGAGAG TGAATTTCTT 
GGAGAGATCC TCATAGAGTT GGAAACAGCG CTTTTAGATG ATGAGCCCCA TTGGTATAAA 
CTCCAGACAC ATGACGAATC TTCACTACCT CTGCCTCAGC CATCACCGTT CATGCCCAGG 
CGGCATATTC ATGGAGAGAG CTCCAGCAAA AAGCTACAAA GATCTCAGCG AATCAGTGAT 
AGTGACATCT CAGATTATGA GGTTGATGAT GGTATTGGAG TAGTGCCTCC AGTGGGTTAT 
AGAGCTAGTG CTAGAGAGAG TAAAGCAACC ACGTTAACAG TGCCAGAGCA ACAAAGAACT 
ACACATCACC GCTCACGTTC CGTGTCTCCT CATCGCGGCG ATGATCAGGG AAGGCCTCGT 
TCACGTTTAC CAAATGTGCC ATTACAGAGG AGCTTAGATG AAATTCATCC AACACGAAGG 
TCACGTTCTC CAACCCGACA CCATGATGCC TCCCGAAGCC CGGCCGATCA CAGATCCAGA 
CATGTGGAAA GTCAATATTC GTCAGAGCCA GACAGTGAGC TTCTCATGCT GCCCAGAGCA 
AAACGAGGAC GAAGTGCAGA AAGCCTACAC ATGACCAGTG AACTGCAGCC CTCTCTTGAC 
AGGGCTAGGA GTGCTAGTAC CAACTGCTTG AGACCAGATA CTAGTTTGCA TTCACCAGAA 
CGAGAAAGGG GTAGATGGTC CCCCTCCCTA GATAGGAGGC GACCTGCTAG CCCCAGGATT 
CAAATCCAGC ATGCATCTCC GGAGAATGAC AGAACGCACC GACAAGGAAG CCCAACCCAG 
TCTCCTCCAG CAGACACATC CTTCGGCAGT CGCCGTGGAA GACAGCTCCC ACAGGTGCCA 
GTTCGAAGCG GCAGTATAGA ACAAGCAAGC TTAGTAGTGG AGGAGCGAAC GAGACAGATG 
AAAATGAAAG TTCACCGATT TAAGCAGACA ACAGGGTCTG GGTCTAGTCA AGAACTTGAC 
CACGAGCAAT ACTCCAAGAA GTCACGTTAC ATTATGGAGT ACAACATACA TAAAGATCAG 
TACAGAAGCT GTGATAACGC GTCTGCCAAG TCTTCAGATA GTGATGTCAG TGATGTGTCC 
GCCATTTCCA GAGCCAGCAG TACCTCACGC CTCAGCAGCA CAAGCTTTAT GTCAGAGCAG 
TCTGAGCGCC CCAGGGGTAG GATCAGTTCA TTTACCCCCA AAATGCAAGG CAGACGGATG 
GGGACTTCAG GAAGAGCCAT CATCAAGAGC ACCAGTGTAA GTGGAGAGAT ATATACACTG 
GAACGTAATG ACGGTAGCCA GTCGGACACG GCCGTGGGTA CCGTCGGAGC CGGTGGAAAG 
AAACGAAGAT CCAGCCTGAG CGCCAAAGTG GTAGCCATTG TGTCTCGAAG AAGCAGGAGC 
ACGTCACAGC TCAGCCAGAC AGAGTCGGGC CACAAGAAGT TGAAAAGCAC CATCCAGAGG 
AGTACGGAAA CAGGAATGGC AGCTGAAATG CGGAAGATGG TGAGACAGCC GAGCCGGGAG 
TCCACGGATG GCAGCATCAA CAGTTATAGC TCGGAAGGAA ACTTGATATT TCCTGGAGTT 
CGAGTAGGAC CCGACAGTCA GTTCAGTGAT TTCCTTGATG GGTTGGGACC AGCGCAGCTC 
GTTGGCCGTC AGACGCTCGC CACCCCGGCC ATGGGCGATA TCCAAATCGG GATGGAGGAT 
AAGAAGGGTC AGTTGGAGGT TGAGGTTATC AGAGCCCGGA GCCTTACACA AAAACCTGGT 
TCCAAATCTA CACCCGCTCC CTATGTGAAA GTATATCTTT TGGAAAATGG AGCCTGTATT 
GCCAAAAAGA AGACAAGAAT TGCACGGAAA ACTCTCGATC CTTTGTATCA GCAGTCCCTG 
GTTTTTGATG AAAGTCCACA GGGTAAAGTT CTTCAGGTGA TTGTCTGGGG TGACTATGGA 
AGAATGGACC ACAAATGCTT TATGGGTGTG GCTCAAATCT TGTTGGAAGA ACTTGATCTA 
TCCAGCATGG TGATTGGATG GTATAAATTG TTCCCTCCGT CCTCACTGGT GGATCCCACT 
CTCGCTCCCC TGACCCGCCG GGCTTCCCAA TCATCTCTGG AAAGTTCGTC CGGGCCTCCC 
TGCATCCGGT CATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017452153.1
CDS	309..5003
Translation	MSSAVGPRGPRPPTVPPPMQELPDLSHLTEEERNIIMAVMDRQKEEEEKEEAMLKCVVRDMAKPAACKTPRNAESQPHQPPLNIFRCVCVPRKPSSEEGGPERDWRLHQQFESYKEQVRKIGEEARRYQGEHKDDAPTCGICHKTKFADGCGHLCSYCRTKFCARCGGRVSLRSNNEDKVVMWVCNLCRKQQEILTKSGAWFFGSGPQQPSQDGTLSDTATGAGSEVPREKKARLQERSRSQTPLSTAAVSSQDTATPGAPLDRNKGAEPSQQALGPEQKQASRSRSEPPRERKKAPGLSEQNGKGGQKSERKRVPKSVVQPGEGIADERERKERRETRRLEKGRSQDYSDRPEKRDNGRVAEDQKQRKEEEYQTRYRSDPNLARYPVKAPPEEQQMRMHARVSRARHERRHSDVALPHTEAAAAAPAEATAGKRAPATARVSPPESPRARAPAAQPPTEHGPPPPRPVPGPAEPPEPRVPEPLRKQGRLDPASAVLLRKAKREKAESMLRNDSLSSDQSESVRPSPPKPHRPKRGGKRRQMSVSSSEEEGVSTPEYTSCEDVELESESVSEKGDLDYYWLDPATWHSRETSPISSHPVTWQPSKEGDRLIGRVILNKRTTMPKESGALLGLKVVGGKMTDLGRLGAFITKVKKGSLADVVGHLRAGDEVLEWNGKPLPGATNEEVYNIILESKSEPQVEIIVSRPIGDIPRIPESSHPPLESSSSSFESQKMERPSISVISPTSPGALKDAPQVLPGQLSVKLWYDKVGHQLIVNVLQATDLPPRVDGRPRNPYVKMYFLPDRSDKSKRRTKTVKKLLEPKWNQTFVYSHVHRRDFRERMLEITVWDQPRVQDEESEFLGEILIELETALLDDEPHWYKLQTHDESSLPLPQPSPFMPRRHIHGESSSKKLQRSQRISDSDISDYEVDDGIGVVPPVGYRASARESKATTLTVPEQQRTTHHRSRSVSPHRGDDQGRPRSRLPNVPLQRSLDEIHPTRRSRSPTRHHDASRSPADHRSRHVESQYSSEPDSELLMLPRAKRGRSAESLHMTSELQPSLDRARSASTNCLRPDTSLHSPERERGRWSPSLDRRRPASPRIQIQHASPENDRTHRQGSPTQSPPADTSFGSRRGRQLPQVPVRSGSIEQASLVVEERTRQMKMKVHRFKQTTGSGSSQELDHEQYSKKSRYIMEYNIHKDQYRSCDNASAKSSDSDVSDVSAISRASSTSRLSSTSFMSEQSERPRGRISSFTPKMQGRRMGTSGRAIIKSTSVSGEIYTLERNDGSQSDTAVGTVGAGGKKRRSSLSAKVVAIVSRRSRSTSQLSQTESGHKKLKSTIQRSTETGMAAEMRKMVRQPSRESTDGSINSYSSEGNLIFPGVRVGPDSQFSDFLDGLGPAQLVGRQTLATPAMGDIQIGMEDKKGQLEVEVIRARSLTQKPGSKSTPAPYVKVYLLENGACIAKKKTRIARKTLDPLYQQSLVFDESPQGKVLQVIVWGDYGRMDHKCFMGVAQILLEELDLSSMVIGWYKLFPPSSLVDPTLAPLTRRASQSSLESSSGPPCIRS

Target ORF information:

RefSeq Version	XM_017596664.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus regulating synaptic membrane exocytosis 1 (Rims1), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017596664.1

ORF Insert Sequence:

ATGTCCTCGG CCGTGGGGCC CCGAGGTCCT CGCCCACCCA CGGTGCCTCC CCCTATGCAA 
GAACTGCCCG ACCTGAGCCA CCTGACCGAG GAGGAGAGGA ACATTATCAT GGCAGTGATG 
GACCGGCAGA AGGAAGAGGA GGAAAAAGAA GAGGCCATGC TCAAGTGTGT TGTCAGGGAC 
ATGGCGAAGC CTGCTGCCTG CAAAACACCA AGAAATGCTG AAAGCCAGCC CCATCAACCA 
CCACTGAACA TTTTCAGATG TGTCTGTGTT CCCAGAAAGC CAAGCAGCGA AGAGGGAGGC 
CCAGAAAGAG ACTGGAGATT GCATCAACAG TTTGAAAGCT ACAAGGAGCA AGTGAGAAAA 
ATCGGAGAGG AAGCGAGGCG TTACCAGGGC GAGCACAAGG ATGATGCCCC GACGTGTGGA 
ATCTGTCATA AGACAAAGTT TGCTGATGGA TGTGGCCATC TCTGCTCCTA TTGTCGCACC 
AAGTTCTGTG CACGCTGCGG AGGCCGTGTG TCTCTGCGAT CGAACAATGA GGACAAAGTG 
GTTATGTGGG TATGCAATTT ATGTCGAAAG CAACAAGAAA TCTTAACGAA ATCTGGAGCG 
TGGTTCTTTG GAAGTGGCCC TCAGCAGCCT AGTCAAGATG GGACTCTGAG TGACACGGCC 
ACAGGTGCTG GATCTGAGGT GCCAAGAGAA AAGAAAGCAA GGCTCCAAGA GCGATCAAGG 
TCTCAGACGC CCTTGAGTAC AGCAGCTGTC TCTTCCCAAG ACACTGCTAC CCCCGGTGCA 
CCGTTGGACA GGAACAAAGG GGCTGAGCCC TCACAGCAAG CCTTGGGTCC TGAACAGAAG 
CAGGCATCAA GATCAAGAAG CGAGCCACCG AGGGAAAGGA AGAAGGCTCC AGGGCTTTCA 
GAGCAGAATG GCAAGGGAGG CCAGAAGAGC GAGCGCAAAC GTGTCCCCAA GTCTGTGGTG 
CAACCCGGGG AAGGGATCGC GGATGAGAGG GAGAGGAAAG AGAGGCGGGA AACCCGCAGG 
TTGGAGAAAG GGCGCTCCCA GGACTACTCA GACCGGCCTG AGAAACGCGA CAATGGCAGG 
GTGGCGGAAG ACCAGAAGCA GAGGAAGGAG GAGGAGTACC AGACTAGGTA CCGCAGCGAC 
CCTAACCTGG CTCGCTACCC GGTGAAGGCG CCGCCAGAGG AGCAGCAGAT GCGCATGCAC 
GCCCGGGTGT CCCGAGCGAG GCACGAGCGG CGCCACAGCG ACGTGGCGCT CCCGCACACC 
GAGGCAGCTG CCGCCGCGCC GGCTGAGGCC ACGGCGGGCA AGCGCGCGCC GGCCACCGCC 
AGGGTCTCTC CCCCGGAGTC CCCGCGCGCA CGCGCGCCCG CCGCCCAGCC TCCCACCGAG 
CACGGGCCAC CGCCGCCGCG GCCAGTCCCG GGTCCCGCAG AGCCACCCGA GCCGCGCGTC 
CCCGAGCCGC TCCGTAAGCA GGGCCGCCTG GACCCGGCCT CGGCCGTGCT TCTGCGCAAG 
GCCAAGCGCG AGAAGGCGGA GAGCATGCTG CGGAACGACT CGCTGAGCTC CGATCAGTCC 
GAGTCCGTGC GGCCATCCCC GCCCAAGCCT CACCGGCCCA AGCGGGGAGG CAAGAGACGT 
CAGATGTCGG TGAGCAGCTC GGAGGAGGAG GGCGTGTCCA CACCGGAGTA CACGAGCTGC 
GAGGACGTGG AGCTGGAGAG CGAGAGCGTG AGCGAGAAAG GTGACTTGGA TTACTACTGG 
TTGGATCCCG CCACGTGGCA CAGCAGGGAA ACGTCGCCTA TCAGTTCGCA TCCTGTAACG 
TGGCAGCCGT CTAAAGAGGG AGATCGACTA ATCGGCCGTG TTATTCTTAA CAAAAGAACA 
ACCATGCCCA AAGAATCAGG TGCATTATTG GGTCTGAAGG TGGTTGGAGG AAAAATGACG 
GACTTAGGGC GCCTTGGTGC TTTCATCACC AAAGTAAAGA AGGGCAGCCT GGCAGACGTC 
GTCGGACACC TAAGAGCAGG GGACGAAGTC CTAGAGTGGA ATGGTAAACC CCTGCCGGGA 
GCAACAAACG AAGAAGTTTA CAACATTATC TTAGAATCAA AATCAGAACC TCAAGTTGAG 
ATTATTGTTT CAAGGCCTAT TGGTGACATC CCCAGGATCC CTGAGAGTTC CCATCCTCCC 
CTGGAGTCCA GTTCAAGTTC CTTTGAATCT CAGAAAATGG AAAGGCCTTC TATTTCTGTT 
ATTTCTCCAA CCAGCCCTGG AGCTCTGAAA GATGCCCCAC AAGTCTTACC AGGGCAACTC 
TCAGTGAAGC TATGGTATGA TAAAGTGGGG CACCAGCTGA TTGTAAATGT TCTACAAGCA 
ACAGATCTAC CCCCTAGAGT AGATGGCCGT CCCAGGAATC CCTATGTAAA AATGTATTTT 
CTTCCAGATA GAAGCGACAA AAGTAAAAGG AGAACCAAAA CAGTAAAGAA ACTTCTAGAG 
CCAAAATGGA ACCAGACATT TGTCTACTCA CACGTACATC GTAGAGATTT TCGAGAGCGA 
ATGTTAGAGA TTACCGTGTG GGACCAGCCG AGAGTACAGG ACGAAGAGAG TGAATTTCTT 
GGAGAGATCC TCATAGAGTT GGAAACAGCG CTTTTAGATG ATGAGCCCCA TTGGTATAAA 
CTCCAGACAC ATGACGAATC TTCACTACCT CTGCCTCAGC CATCACCGTT CATGCCCAGG 
CGGCATATTC ATGGAGAGAG CTCCAGCAAA AAGCTACAAA GATCTCAGCG AATCAGTGAT 
AGTGACATCT CAGATTATGA GGTTGATGAT GGTATTGGAG TAGTGCCTCC AGTGGGTTAT 
AGAGCTAGTG CTAGAGAGAG TAAAGCAACC ACGTTAACAG TGCCAGAGCA ACAAAGAACT 
ACACATCACC GCTCACGTTC CGTGTCTCCT CATCGCGGCG ATGATCAGGG AAGGCCTCGT 
TCACGTTTAC CAAATGTGCC ATTACAGAGG AGCTTAGATG AAATTCATCC AACACGAAGG 
TCACGTTCTC CAACCCGACA CCATGATGCC TCCCGAAGCC CGGCCGATCA CAGATCCAGA 
CATGTGGAAA GTCAATATTC GTCAGAGCCA GACAGTGAGC TTCTCATGCT GCCCAGAGCA 
AAACGAGGAC GAAGTGCAGA AAGCCTACAC ATGACCAGTG AACTGCAGCC CTCTCTTGAC 
AGGGCTAGGA GTGCTAGTAC CAACTGCTTG AGACCAGATA CTAGTTTGCA TTCACCAGAA 
CGAGAAAGGG GTAGATGGTC CCCCTCCCTA GATAGGAGGC GACCTGCTAG CCCCAGGATT 
CAAATCCAGC ATGCATCTCC GGAGAATGAC AGAACGCACC GACAAGGAAG CCCAACCCAG 
TCTCCTCCAG CAGACACATC CTTCGGCAGT CGCCGTGGAA GACAGCTCCC ACAGGTGCCA 
GTTCGAAGCG GCAGTATAGA ACAAGCAAGC TTAGTAGTGG AGGAGCGAAC GAGACAGATG 
AAAATGAAAG TTCACCGATT TAAGCAGACA ACAGGGTCTG GGTCTAGTCA AGAACTTGAC 
CACGAGCAAT ACTCCAAGAA GTCACGTTAC ATTATGGAGT ACAACATACA TAAAGATCAG 
TACAGAAGCT GTGATAACGC GTCTGCCAAG TCTTCAGATA GTGATGTCAG TGATGTGTCC 
GCCATTTCCA GAGCCAGCAG TACCTCACGC CTCAGCAGCA CAAGCTTTAT GTCAGAGCAG 
TCTGAGCGCC CCAGGGGTAG GATCAGTTCA TTTACCCCCA AAATGCAAGG CAGACGGATG 
GGGACTTCAG GAAGAGCCAT CATCAAGAGC ACCAGTGTAA GTGGAGAGAT ATATACACTG 
GAACGTAATG ACGGTAGCCA GTCGGACACG GCCGTGGGTA CCGTCGGAGC CGGTGGAAAG 
AAACGAAGAT CCAGCCTGAG CGCCAAAGTG GTAGCCATTG TGTCTCGAAG AAGCAGGAGC 
ACGTCACAGC TCAGCCAGAC AGAGTCGGGC CACAAGAAGT TGAAAAGCAC CATCCAGAGG 
AGTACGGAAA CAGGAATGGC AGCTGAAATG CGGAAGATGG TGAGACAGCC GAGCCGGGAG 
TCCACGGATG GCAGCATCAA CAGTTATAGC TCGGAAGGAA ACTTGATATT TCCTGGAGTT 
CGAGTAGGAC CCGACAGTCA GTTCAGTGAT TTCCTTGATG GGTTGGGACC AGCGCAGCTC 
GTTGGCCGTC AGACGCTCGC CACCCCGGCC ATGGGCGATA TCCAAATCGG GATGGAGGAT 
AAGAAGGGTC AGTTGGAGGT TGAGGTTATC AGAGCCCGGA GCCTTACACA AAAACCTGGT 
TCCAAATCTA CACCCGCTCC CTATGTGAAA GTATATCTTT TGGAAAATGG AGCCTGTATT 
GCCAAAAAGA AGACAAGAAT TGCACGGAAA ACTCTCGATC CTTTGTATCA GCAGTCCCTG 
GTTTTTGATG AAAGTCCACA GGGTAAAGTT CTTCAGGTGA TTGTCTGGGG TGACTATGGA 
AGAATGGACC ACAAATGCTT TATGGGTGTG GCTCAAATCT TGTTGGAAGA ACTTGATCTA 
TCCAGCATGG TGATTGGATG GTATAAATTG TTCCCTCCGT CCTCACTGGT GGATCCCACT 
CTCGCTCCCC TGACCCGCCG GGCTTCCCAA TCATCTCTGG AAAGTTCGTC CGGGCCTCCC 
TGCATCCGGT CATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rims1 ( XM_017596665.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017452154.1 Rattus norvegicus regulating synaptic membrane exocytosis 1 (Rims1), transcript variant X12, mRNA.

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CloneID	ORa62882
Clone ID Related Accession (Same CDS sequence)	XM_017596665.1
Accession Version	XM_017596665.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4683bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	regulating synaptic membrane exocytosis protein 1 isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005108.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCTCGG CCGTGGGGCC CCGAGGTCCT CGCCCACCCA CGGTGCCTCC CCCTATGCAA 
GAACTGCCCG ACCTGAGCCA CCTGACCGAG GAGGAGAGGA ACATTATCAT GGCAGTGATG 
GACCGGCAGA AGGAAGAGGA GGAAAAAGAA GAGGCCATGC TCAAGTGTGT TGTCAGGGAC 
ATGGCGAAGC CTGCTGCCTG CAAAACACCA AGAAATGCTG AAAGCCAGCC CCATCAACCA 
CCACTGAACA TTTTCAGATG TGTCTGTGTT CCCAGAAAGC CAAGCAGCGA AGAGGGAGGC 
CCAGAAAGAG ACTGGAGATT GCATCAACAG TTTGAAAGCT ACAAGGAGCA AGTGAGAAAA 
ATCGGAGAGG AAGCGAGGCG TTACCAGGGC GAGCACAAGG ATGATGCCCC GACGTGTGGA 
ATCTGTCATA AGACAAAGTT TGCTGATGGA TGTGGCCATC TCTGCTCCTA TTGTCGCACC 
AAGTTCTGTG CACGCTGCGG AGGCCGTGTG TCTCTGCGAT CGAACAATGA GGACAAAGTG 
GTTATGTGGG TATGCAATTT ATGTCGAAAG CAACAAGAAA TCTTAACGAA ATCTGGAGCG 
TGGTTCTTTG GAAGTGGCCC TCAGCAGCCT AGTCAAGATG GGACTCTGAG TGACACGGCC 
ACAGGTGCTG GATCTGAGGT GCCAAGAGAA AAGAAAGCAA GGCTCCAAGA GCGATCAAGG 
TCTCAGACGC CCTTGAGTAC AGCAGCTGTC TCTTCCCAAG ACACTGCTAC CCCCGGTGCA 
CCGTTGGACA GGAACAAAGG GGCTGAGCCC TCACAGCAAG CCTTGGGTCC TGAACAGAAG 
CAGGCATCAA GATCAAGAAG CGAGCCACCG AGGGAAAGGA AGAAGGCTCC AGGGCTTTCA 
GAGCAGAATG GCAAGGGAGG CCAGAAGAGC GAGCGCAAAC GTGTCCCCAA GTCTGTGGTG 
CAACCCGGGG AAGGGATCGC GGATGAGAGG GAGAGGAAAG AGAGGCGGGA AACCCGCAGG 
TTGGAGAAAG GGCGCTCCCA GGACTACTCA GACCGGCCTG AGAAACGCGA CAATGGCAGG 
GTGGCGGAAG ACCAGAAGCA GAGGAAGGAG GAGGAGTACC AGACTAGGTA CCGCAGCGAC 
CCTAACCTGG CTCGCTACCC GGTGAAGGCG CCGCCAGAGG AGCAGCAGAT GCGCATGCAC 
GCCCGGGTGT CCCGAGCGAG GCACGAGCGG CGCCACAGCG ACGTGGCGCT CCCGCACACC 
GAGGCAGCTG CCGCCGCGCC GGCTGAGGCC ACGGCGGGCA AGCGCGCGCC GGCCACCGCC 
AGGGTCTCTC CCCCGGAGTC CCCGCGCGCA CGCGCGCCCG CCGCCCAGCC TCCCACCGAG 
CACGGGCCAC CGCCGCCGCG GCCAGTCCCG GGTCCCGCAG AGCCACCCGA GCCGCGCGTC 
CCCGAGCCGC TCCGTAAGCA GGGCCGCCTG GACCCGGCCT CGGCCGTGCT TCTGCGCAAG 
GCCAAGCGCG AGAAGGCGGA GAGCATGCTG CGGAACGACT CGCTGAGCTC CGATCAGTCC 
GAGTCCGTGC GGCCATCCCC GCCCAAGCCT CACCGGCCCA AGCGGGGAGG CAAGAGACGT 
CAGATGTCGG TGAGCAGCTC GGAGGAGGAG GGCGTGTCCA CACCGGAGTA CACGAGCTGC 
GAGGACGTGG AGCTGGAGAG CGAGAGCGTG AGCGAGAAAG GTGACTTGGA TTACTACTGG 
TTGGATCCCG CCACGTGGCA CAGCAGGGAA ACGTCGCCTA TCAGTTCGCA TCCTGTAACG 
TGGCAGCCGT CTAAAGAGGG AGATCGACTA ATCGGCCGTG TTATTCTTAA CAAAAGAACA 
ACCATGCCCA AAGAATCAGG TGCATTATTG GGTCTGAAGG TGGTTGGAGG AAAAATGACG 
GACTTAGGGC GCCTTGGTGC TTTCATCACC AAAGTAAAGA AGGGCAGCCT GGCAGACGTC 
GTCGGACACC TAAGAGCAGG GGACGAAGTC CTAGAGTGGA ATGGTAAACC CCTGCCGGGA 
GCAACAAACG AAGAAGTTTA CAACATTATC TTAGAATCAA AATCAGAACC TCAAGTTGAG 
ATTATTGTTT CAAGGCCTAT TGGTGACATC CCCAGGATCC CTGAGAGTTC CCATCCTCCC 
CTGGAGTCCA GTTCAAGTTC CTTTGAATCT CAGAAAATGG AAAGGCCTTC TATTTCTGTT 
ATTTCTCCAA CCAGCCCTGG AGCTCTGAAA GATGCCCCAC AAGTCTTACC AGGGCAACTC 
TCAGTGAAGC TATGGTATGA TAAAGTGGGG CACCAGCTGA TTGTAAATGT TCTACAAGCA 
ACAGATCTAC CCCCTAGAGT AGATGGCCGT CCCAGGAATC CCTATGTAAA AATGTATTTT 
CTTCCAGATA GAAGCGACAA AAGTAAAAGG AGAACCAAAA CAGTAAAGAA ACTTCTAGAG 
CCAAAATGGA ACCAGACATT TGTCTACTCA CACGTACATC GTAGAGATTT TCGAGAGCGA 
ATGTTAGAGA TTACCGTGTG GGACCAGCCG AGAGTACAGG ACGAAGAGAG TGAATTTCTT 
GGAGAGATCC TCATAGAGTT GGAAACAGCG CTTTTAGATG ATGAGCCCCA TTGGTATAAA 
CTCCAGACAC ATGACGAATC TTCACTACCT CTGCCTCAGC CATCACCGTT CATGCCCAGG 
CGGCATATTC ATGGAGAGAG CTCCAGCAAA AAGCTACAAA GATCTCAGCG AATCAGTGAT 
AGTGACATCT CAGATTATGA GGTTGATGAT GGTATTGGAG TAGTGCCTCC AGTGGGTTAT 
AGAGCTAGTG CTAGAGAGAG TAAAGCAACC ACGTTAACAG TGCCAGAGCA ACAAAGAACT 
ACACATCACC GCTCACGTTC CGTGTCTCCT CATCGCGGCG ATGATCAGGG AAGGCCTCGT 
TCACGTTTAC CAAATGTGCC ATTACAGAGG AGCTTAGATG AAATTCATCC AACACGAAGG 
TCACGTTCTC CAACCCGACA CCATGATGCC TCCCGAAGCC CGGCCGATCA CAGATCCAGA 
CATGTGGAAA GTCAATATTC GTCAGAGCCA GACAGTGAGC TTCTCATGCT GCCCAGAGCA 
AAACGAGGAC GAAGTGCAGA AAGCCTACAC ATGACCAGTG AACTGCAGCC CTCTCTTGAC 
AGGGCTAGGA GTGCTAGTAC CAACTGCTTG AGACCAGATA CTAGTTTGCA TTCACCAGAA 
CGAGAAAGGC ACTCCAGAAA GTCTGAAAGA TGTAGCATCC AAAAACAGTC TAGGAAAGGC 
ACAGCCTCTG ATGCAGACAG AACGCACCGA CAAGGAAGCC CAACCCAGTC TCCTCCAGCA 
GACACATCCT TCGGCAGTCG CCGTGGAAGA CAGCTCCCAC AGGTGCCAGT TCGAAGCGGC 
AGTATAGAAC AAGCAAGCTT AGTAGTGGAG GAGCGAACGA GACAGATGAA AATGAAAGTT 
CACCGATTTA AGCAGACAAC AGGGTCTGGG TCTAGTCAAG AACTTGACCA CGAGCAATAC 
TCCAAGAAGT CACGTTACAT TATGGAGTAC AACATACATA AAGATCAGTA CAGAAGCTGT 
GATAACGCGT CTGCCAAGTC TTCAGATAGT GATGTCAGTG ATGTGTCCGC CATTTCCAGA 
GCCAGCAGTA CCTCACGCCT CAGCAGCACA AGCTTTATGT CAGAGCAGTC TGAGCGCCCC 
AGGGGTAGGA TCAGTTCATT TACCCCCAAA ATGCAAGGCA GACGGATGGG GACTTCAGGA 
AGAGCCATCA TCAAGAGCAC CAGTGTAAGT GGAGAGATAT ATACACTGGA ACGTAATGAC 
GGTAGCCAGT CGGACACGGC CGTGGGTACC GTCGGAGCCG GTGGAAAGAA ACGAAGATCC 
AGCCTGAGCG CCAAAGTGGT AGCCATTGTG TCTCGAAGAA GCAGGAGCAC GTCACAGCTC 
AGCCAGACAG AGTCGGGCCA CAAGAAGTTG AAAAGCACCA TCCAGAGGAG TACGGAAACA 
GGAATGGCAG CTGAAATGCG GAAGATGGTG AGACAGCCGA GCCGGGAGTC CACGGATGGC 
AGCATCAACA GTTATAGCTC GGAAGGAAAC TTGATATTTC CTGGAGTTCG AGTAGGACCC 
GACAGTCAGT TCAGTGATTT CCTTGATGGG TTGGGACCAG CGCAGCTCGT TGGCCGTCAG 
ACGCTCGCCA CCCCGGCCAT GGGCGATATC CAAATCGGGA TGGAGGATAA GAAGGGTCAG 
TTGGAGGTTG AGGTTATCAG AGCCCGGAGC CTTACACAAA AACCTGGTTC CAAATCTACA 
CCCGCTCCCT ATGTGAAAGT ATATCTTTTG GAAAATGGAG CCTGTATTGC CAAAAAGAAG 
ACAAGAATTG CACGGAAAAC TCTCGATCCT TTGTATCAGC AGTCCCTGGT TTTTGATGAA 
AGTCCACAGG GTAAAGTTCT TCAGGTGATT GTCTGGGGTG ACTATGGAAG AATGGACCAC 
AAATGCTTTA TGGGTGTGGC TCAAATCTTG TTGGAAGAAC TTGATCTATC CAGCATGGTG 
ATTGGATGGT ATAAATTGTT CCCTCCGTCC TCACTGGTGG ATCCCACTCT CGCTCCCCTG 
ACCCGCCGGG CTTCCCAATC ATCTCTGGAA AGTTCGTCCG GGCCTCCCTG CATCCGGTCA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017452154.1
CDS	309..4991
Translation	MSSAVGPRGPRPPTVPPPMQELPDLSHLTEEERNIIMAVMDRQKEEEEKEEAMLKCVVRDMAKPAACKTPRNAESQPHQPPLNIFRCVCVPRKPSSEEGGPERDWRLHQQFESYKEQVRKIGEEARRYQGEHKDDAPTCGICHKTKFADGCGHLCSYCRTKFCARCGGRVSLRSNNEDKVVMWVCNLCRKQQEILTKSGAWFFGSGPQQPSQDGTLSDTATGAGSEVPREKKARLQERSRSQTPLSTAAVSSQDTATPGAPLDRNKGAEPSQQALGPEQKQASRSRSEPPRERKKAPGLSEQNGKGGQKSERKRVPKSVVQPGEGIADERERKERRETRRLEKGRSQDYSDRPEKRDNGRVAEDQKQRKEEEYQTRYRSDPNLARYPVKAPPEEQQMRMHARVSRARHERRHSDVALPHTEAAAAAPAEATAGKRAPATARVSPPESPRARAPAAQPPTEHGPPPPRPVPGPAEPPEPRVPEPLRKQGRLDPASAVLLRKAKREKAESMLRNDSLSSDQSESVRPSPPKPHRPKRGGKRRQMSVSSSEEEGVSTPEYTSCEDVELESESVSEKGDLDYYWLDPATWHSRETSPISSHPVTWQPSKEGDRLIGRVILNKRTTMPKESGALLGLKVVGGKMTDLGRLGAFITKVKKGSLADVVGHLRAGDEVLEWNGKPLPGATNEEVYNIILESKSEPQVEIIVSRPIGDIPRIPESSHPPLESSSSSFESQKMERPSISVISPTSPGALKDAPQVLPGQLSVKLWYDKVGHQLIVNVLQATDLPPRVDGRPRNPYVKMYFLPDRSDKSKRRTKTVKKLLEPKWNQTFVYSHVHRRDFRERMLEITVWDQPRVQDEESEFLGEILIELETALLDDEPHWYKLQTHDESSLPLPQPSPFMPRRHIHGESSSKKLQRSQRISDSDISDYEVDDGIGVVPPVGYRASARESKATTLTVPEQQRTTHHRSRSVSPHRGDDQGRPRSRLPNVPLQRSLDEIHPTRRSRSPTRHHDASRSPADHRSRHVESQYSSEPDSELLMLPRAKRGRSAESLHMTSELQPSLDRARSASTNCLRPDTSLHSPERERHSRKSERCSIQKQSRKGTASDADRTHRQGSPTQSPPADTSFGSRRGRQLPQVPVRSGSIEQASLVVEERTRQMKMKVHRFKQTTGSGSSQELDHEQYSKKSRYIMEYNIHKDQYRSCDNASAKSSDSDVSDVSAISRASSTSRLSSTSFMSEQSERPRGRISSFTPKMQGRRMGTSGRAIIKSTSVSGEIYTLERNDGSQSDTAVGTVGAGGKKRRSSLSAKVVAIVSRRSRSTSQLSQTESGHKKLKSTIQRSTETGMAAEMRKMVRQPSRESTDGSINSYSSEGNLIFPGVRVGPDSQFSDFLDGLGPAQLVGRQTLATPAMGDIQIGMEDKKGQLEVEVIRARSLTQKPGSKSTPAPYVKVYLLENGACIAKKKTRIARKTLDPLYQQSLVFDESPQGKVLQVIVWGDYGRMDHKCFMGVAQILLEELDLSSMVIGWYKLFPPSSLVDPTLAPLTRRASQSSLESSSGPPCIRS

Target ORF information:

RefSeq Version	XM_017596665.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus regulating synaptic membrane exocytosis 1 (Rims1), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017596665.1

ORF Insert Sequence:

ATGTCCTCGG CCGTGGGGCC CCGAGGTCCT CGCCCACCCA CGGTGCCTCC CCCTATGCAA 
GAACTGCCCG ACCTGAGCCA CCTGACCGAG GAGGAGAGGA ACATTATCAT GGCAGTGATG 
GACCGGCAGA AGGAAGAGGA GGAAAAAGAA GAGGCCATGC TCAAGTGTGT TGTCAGGGAC 
ATGGCGAAGC CTGCTGCCTG CAAAACACCA AGAAATGCTG AAAGCCAGCC CCATCAACCA 
CCACTGAACA TTTTCAGATG TGTCTGTGTT CCCAGAAAGC CAAGCAGCGA AGAGGGAGGC 
CCAGAAAGAG ACTGGAGATT GCATCAACAG TTTGAAAGCT ACAAGGAGCA AGTGAGAAAA 
ATCGGAGAGG AAGCGAGGCG TTACCAGGGC GAGCACAAGG ATGATGCCCC GACGTGTGGA 
ATCTGTCATA AGACAAAGTT TGCTGATGGA TGTGGCCATC TCTGCTCCTA TTGTCGCACC 
AAGTTCTGTG CACGCTGCGG AGGCCGTGTG TCTCTGCGAT CGAACAATGA GGACAAAGTG 
GTTATGTGGG TATGCAATTT ATGTCGAAAG CAACAAGAAA TCTTAACGAA ATCTGGAGCG 
TGGTTCTTTG GAAGTGGCCC TCAGCAGCCT AGTCAAGATG GGACTCTGAG TGACACGGCC 
ACAGGTGCTG GATCTGAGGT GCCAAGAGAA AAGAAAGCAA GGCTCCAAGA GCGATCAAGG 
TCTCAGACGC CCTTGAGTAC AGCAGCTGTC TCTTCCCAAG ACACTGCTAC CCCCGGTGCA 
CCGTTGGACA GGAACAAAGG GGCTGAGCCC TCACAGCAAG CCTTGGGTCC TGAACAGAAG 
CAGGCATCAA GATCAAGAAG CGAGCCACCG AGGGAAAGGA AGAAGGCTCC AGGGCTTTCA 
GAGCAGAATG GCAAGGGAGG CCAGAAGAGC GAGCGCAAAC GTGTCCCCAA GTCTGTGGTG 
CAACCCGGGG AAGGGATCGC GGATGAGAGG GAGAGGAAAG AGAGGCGGGA AACCCGCAGG 
TTGGAGAAAG GGCGCTCCCA GGACTACTCA GACCGGCCTG AGAAACGCGA CAATGGCAGG 
GTGGCGGAAG ACCAGAAGCA GAGGAAGGAG GAGGAGTACC AGACTAGGTA CCGCAGCGAC 
CCTAACCTGG CTCGCTACCC GGTGAAGGCG CCGCCAGAGG AGCAGCAGAT GCGCATGCAC 
GCCCGGGTGT CCCGAGCGAG GCACGAGCGG CGCCACAGCG ACGTGGCGCT CCCGCACACC 
GAGGCAGCTG CCGCCGCGCC GGCTGAGGCC ACGGCGGGCA AGCGCGCGCC GGCCACCGCC 
AGGGTCTCTC CCCCGGAGTC CCCGCGCGCA CGCGCGCCCG CCGCCCAGCC TCCCACCGAG 
CACGGGCCAC CGCCGCCGCG GCCAGTCCCG GGTCCCGCAG AGCCACCCGA GCCGCGCGTC 
CCCGAGCCGC TCCGTAAGCA GGGCCGCCTG GACCCGGCCT CGGCCGTGCT TCTGCGCAAG 
GCCAAGCGCG AGAAGGCGGA GAGCATGCTG CGGAACGACT CGCTGAGCTC CGATCAGTCC 
GAGTCCGTGC GGCCATCCCC GCCCAAGCCT CACCGGCCCA AGCGGGGAGG CAAGAGACGT 
CAGATGTCGG TGAGCAGCTC GGAGGAGGAG GGCGTGTCCA CACCGGAGTA CACGAGCTGC 
GAGGACGTGG AGCTGGAGAG CGAGAGCGTG AGCGAGAAAG GTGACTTGGA TTACTACTGG 
TTGGATCCCG CCACGTGGCA CAGCAGGGAA ACGTCGCCTA TCAGTTCGCA TCCTGTAACG 
TGGCAGCCGT CTAAAGAGGG AGATCGACTA ATCGGCCGTG TTATTCTTAA CAAAAGAACA 
ACCATGCCCA AAGAATCAGG TGCATTATTG GGTCTGAAGG TGGTTGGAGG AAAAATGACG 
GACTTAGGGC GCCTTGGTGC TTTCATCACC AAAGTAAAGA AGGGCAGCCT GGCAGACGTC 
GTCGGACACC TAAGAGCAGG GGACGAAGTC CTAGAGTGGA ATGGTAAACC CCTGCCGGGA 
GCAACAAACG AAGAAGTTTA CAACATTATC TTAGAATCAA AATCAGAACC TCAAGTTGAG 
ATTATTGTTT CAAGGCCTAT TGGTGACATC CCCAGGATCC CTGAGAGTTC CCATCCTCCC 
CTGGAGTCCA GTTCAAGTTC CTTTGAATCT CAGAAAATGG AAAGGCCTTC TATTTCTGTT 
ATTTCTCCAA CCAGCCCTGG AGCTCTGAAA GATGCCCCAC AAGTCTTACC AGGGCAACTC 
TCAGTGAAGC TATGGTATGA TAAAGTGGGG CACCAGCTGA TTGTAAATGT TCTACAAGCA 
ACAGATCTAC CCCCTAGAGT AGATGGCCGT CCCAGGAATC CCTATGTAAA AATGTATTTT 
CTTCCAGATA GAAGCGACAA AAGTAAAAGG AGAACCAAAA CAGTAAAGAA ACTTCTAGAG 
CCAAAATGGA ACCAGACATT TGTCTACTCA CACGTACATC GTAGAGATTT TCGAGAGCGA 
ATGTTAGAGA TTACCGTGTG GGACCAGCCG AGAGTACAGG ACGAAGAGAG TGAATTTCTT 
GGAGAGATCC TCATAGAGTT GGAAACAGCG CTTTTAGATG ATGAGCCCCA TTGGTATAAA 
CTCCAGACAC ATGACGAATC TTCACTACCT CTGCCTCAGC CATCACCGTT CATGCCCAGG 
CGGCATATTC ATGGAGAGAG CTCCAGCAAA AAGCTACAAA GATCTCAGCG AATCAGTGAT 
AGTGACATCT CAGATTATGA GGTTGATGAT GGTATTGGAG TAGTGCCTCC AGTGGGTTAT 
AGAGCTAGTG CTAGAGAGAG TAAAGCAACC ACGTTAACAG TGCCAGAGCA ACAAAGAACT 
ACACATCACC GCTCACGTTC CGTGTCTCCT CATCGCGGCG ATGATCAGGG AAGGCCTCGT 
TCACGTTTAC CAAATGTGCC ATTACAGAGG AGCTTAGATG AAATTCATCC AACACGAAGG 
TCACGTTCTC CAACCCGACA CCATGATGCC TCCCGAAGCC CGGCCGATCA CAGATCCAGA 
CATGTGGAAA GTCAATATTC GTCAGAGCCA GACAGTGAGC TTCTCATGCT GCCCAGAGCA 
AAACGAGGAC GAAGTGCAGA AAGCCTACAC ATGACCAGTG AACTGCAGCC CTCTCTTGAC 
AGGGCTAGGA GTGCTAGTAC CAACTGCTTG AGACCAGATA CTAGTTTGCA TTCACCAGAA 
CGAGAAAGGC ACTCCAGAAA GTCTGAAAGA TGTAGCATCC AAAAACAGTC TAGGAAAGGC 
ACAGCCTCTG ATGCAGACAG AACGCACCGA CAAGGAAGCC CAACCCAGTC TCCTCCAGCA 
GACACATCCT TCGGCAGTCG CCGTGGAAGA CAGCTCCCAC AGGTGCCAGT TCGAAGCGGC 
AGTATAGAAC AAGCAAGCTT AGTAGTGGAG GAGCGAACGA GACAGATGAA AATGAAAGTT 
CACCGATTTA AGCAGACAAC AGGGTCTGGG TCTAGTCAAG AACTTGACCA CGAGCAATAC 
TCCAAGAAGT CACGTTACAT TATGGAGTAC AACATACATA AAGATCAGTA CAGAAGCTGT 
GATAACGCGT CTGCCAAGTC TTCAGATAGT GATGTCAGTG ATGTGTCCGC CATTTCCAGA 
GCCAGCAGTA CCTCACGCCT CAGCAGCACA AGCTTTATGT CAGAGCAGTC TGAGCGCCCC 
AGGGGTAGGA TCAGTTCATT TACCCCCAAA ATGCAAGGCA GACGGATGGG GACTTCAGGA 
AGAGCCATCA TCAAGAGCAC CAGTGTAAGT GGAGAGATAT ATACACTGGA ACGTAATGAC 
GGTAGCCAGT CGGACACGGC CGTGGGTACC GTCGGAGCCG GTGGAAAGAA ACGAAGATCC 
AGCCTGAGCG CCAAAGTGGT AGCCATTGTG TCTCGAAGAA GCAGGAGCAC GTCACAGCTC 
AGCCAGACAG AGTCGGGCCA CAAGAAGTTG AAAAGCACCA TCCAGAGGAG TACGGAAACA 
GGAATGGCAG CTGAAATGCG GAAGATGGTG AGACAGCCGA GCCGGGAGTC CACGGATGGC 
AGCATCAACA GTTATAGCTC GGAAGGAAAC TTGATATTTC CTGGAGTTCG AGTAGGACCC 
GACAGTCAGT TCAGTGATTT CCTTGATGGG TTGGGACCAG CGCAGCTCGT TGGCCGTCAG 
ACGCTCGCCA CCCCGGCCAT GGGCGATATC CAAATCGGGA TGGAGGATAA GAAGGGTCAG 
TTGGAGGTTG AGGTTATCAG AGCCCGGAGC CTTACACAAA AACCTGGTTC CAAATCTACA 
CCCGCTCCCT ATGTGAAAGT ATATCTTTTG GAAAATGGAG CCTGTATTGC CAAAAAGAAG 
ACAAGAATTG CACGGAAAAC TCTCGATCCT TTGTATCAGC AGTCCCTGGT TTTTGATGAA 
AGTCCACAGG GTAAAGTTCT TCAGGTGATT GTCTGGGGTG ACTATGGAAG AATGGACCAC 
AAATGCTTTA TGGGTGTGGC TCAAATCTTG TTGGAAGAAC TTGATCTATC CAGCATGGTG 
ATTGGATGGT ATAAATTGTT CCCTCCGTCC TCACTGGTGG ATCCCACTCT CGCTCCCCTG 
ACCCGCCGGG CTTCCCAATC ATCTCTGGAA AGTTCGTCCG GGCCTCCCTG CATCCGGTCA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rims1 ( XM_017596666.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017452155.1 Rattus norvegicus regulating synaptic membrane exocytosis 1 (Rims1), transcript variant X13, mRNA.

Price & Availability↑

CloneID	ORa62883
Clone ID Related Accession (Same CDS sequence)	XM_017596666.1
Accession Version	XM_017596666.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4611bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	regulating synaptic membrane exocytosis protein 1 isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005108.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCTCGG CCGTGGGGCC CCGAGGTCCT CGCCCACCCA CGGTGCCTCC CCCTATGCAA 
GAACTGCCCG ACCTGAGCCA CCTGACCGAG GAGGAGAGGA ACATTATCAT GGCAGTGATG 
GACCGGCAGA AGGAAGAGGA GGAAAAAGAA GAGGCCATGC TCAAGTGTGT TGTCAGGGAC 
ATGGCGAAGC CTGCTGCCTG CAAAACACCA AGAAATGCTG AAAGCCAGCC CCATCAACCA 
CCACTGAACA TTTTCAGATG TGTCTGTGTT CCCAGAAAGC CAAGCAGCGA AGAGGGAGGC 
CCAGAAAGAG ACTGGAGATT GCATCAACAG TTTGAAAGCT ACAAGGAGCA AGTGAGAAAA 
ATCGGAGAGG AAGCGAGGCG TTACCAGGGC GAGCACAAGG ATGATGCCCC GACGTGTGGA 
ATCTGTCATA AGACAAAGTT TGCTGATGGA TGTGGCCATC TCTGCTCCTA TTGTCGCACC 
AAGTTCTGTG CACGCTGCGG AGGCCGTGTG TCTCTGCGAT CGAACAATGA GGACAAAGTG 
GTTATGTGGG TATGCAATTT ATGTCGAAAG CAACAAGAAA TCTTAACGAA ATCTGGAGCG 
TGGTTCTTTG GAAGTGGCCC TCAGCAGCCT AGTCAAGATG GGACTCTGAG TGACACGGCC 
ACAGGTGCTG GATCTGAGGT GCCAAGAGAA AAGAAAGCAA GGCTCCAAGA GCGATCAAGG 
TCTCAGACGC CCTTGAGTAC AGCAGCTGTC TCTTCCCAAG ACACTGCTAC CCCCGGTGCA 
CCGTTGGACA GGAACAAAGG GGCTGAGCCC TCACAGCAAG CCTTGGGTCC TGAACAGAAG 
CAGGCATCAA GATCAAGAAG CGAGCCACCG AGGGAAAGGA AGAAGGCTCC AGGGCTTTCA 
GAGCAGAATG GCAAGGGAGG CCAGAAGAGC GAGCGCAAAC GTGTCCCCAA GTCTGTGGTG 
CAACCCGGGG AAGGGATCGC GGATGAGAGG GAGAGGAAAG AGAGGCGGGA AACCCGCAGG 
TTGGAGAAAG GGCGCTCCCA GGACTACTCA GACCGGCCTG AGAAACGCGA CAATGGCAGG 
GTGGCGGAAG ACCAGAAGCA GAGGAAGGAG GAGGAGTACC AGACTAGGTA CCGCAGCGAC 
CCTAACCTGG CTCGCTACCC GGTGAAGGCG CCGCCAGAGG AGCAGCAGAT GCGCATGCAC 
GCCCGGGTGT CCCGAGCGAG GCACGAGCGG CGCCACAGCG ACGTGGCGCT CCCGCACACC 
GAGGCAGCTG CCGCCGCGCC GGCTGAGGCC ACGGCGGGCA AGCGCGCGCC GGCCACCGCC 
AGGGTCTCTC CCCCGGAGTC CCCGCGCGCA CGCGCGCCCG CCGCCCAGCC TCCCACCGAG 
CACGGGCCAC CGCCGCCGCG GCCAGTCCCG GGTCCCGCAG AGCCACCCGA GCCGCGCGTC 
CCCGAGCCGC TCCGTAAGCA GGGCCGCCTG GACCCGGCCT CGGCCGTGCT TCTGCGCAAG 
GCCAAGCGCG AGAAGGCGGA GAGCATGCTG CGGAACGACT CGCTGAGCTC CGATCAGTCC 
GAGTCCGTGC GGCCATCCCC GCCCAAGCCT CACCGGCCCA AGCGGGGAGG CAAGAGACGT 
CAGATGTCGG TGAGCAGCTC GGAGGAGGAG GGCGTGTCCA CACCGGAGTA CACGAGCTGC 
GAGGACGTGG AGCTGGAGAG CGAGAGCGTG AGCGAGAAAG GTGACTTGGA TTACTACTGG 
TTGGATCCCG CCACGTGGCA CAGCAGGGAA ACGTCGCCTA TCAGTTCGCA TCCTGTAACG 
TGGCAGCCGT CTAAAGAGGG AGATCGACTA ATCGGCCGTG TTATTCTTAA CAAAAGAACA 
ACCATGCCCA AAGAATCAGG TGCATTATTG GGTCTGAAGG TGGTTGGAGG AAAAATGACG 
GACTTAGGGC GCCTTGGTGC TTTCATCACC AAAGTAAAGA AGGGCAGCCT GGCAGACGTC 
GTCGGACACC TAAGAGCAGG GGACGAAGTC CTAGAGTGGA ATGGTAAACC CCTGCCGGGA 
GCAACAAACG AAGAAGTTTA CAACATTATC TTAGAATCAA AATCAGAACC TCAAGTTGAG 
ATTATTGTTT CAAGGCCTAT TGGTGACATC CCCAGGATCC CTGAGAGTTC CCATCCTCCC 
CTGGAGTCCA GTTCAAGTTC CTTTGAATCT CAGAAAATGG AAAGGCCTTC TATTTCTGTT 
ATTTCTCCAA CCAGCCCTGG AGCTCTGAAA GATGCCCCAC AAGTCTTACC AGGGCAACTC 
TCAGTGAAGC TATGGTATGA TAAAGTGGGG CACCAGCTGA TTGTAAATGT TCTACAAGCA 
ACAGATCTAC CCCCTAGAGT AGATGGCCGT CCCAGGAATC CCTATGTAAA AATGTATTTT 
CTTCCAGATA GAAGCGACAA AAGTAAAAGG AGAACCAAAA CAGTAAAGAA ACTTCTAGAG 
CCAAAATGGA ACCAGACATT TGTCTACTCA CACGTACATC GTAGAGATTT TCGAGAGCGA 
ATGTTAGAGA TTACCGTGTG GGACCAGCCG AGAGTACAGG ACGAAGAGAG TGAATTTCTT 
GGAGAGATCC TCATAGAGTT GGAAACAGCG CTTTTAGATG ATGAGCCCCA TTGGTATAAA 
CTCCAGACAC ATGACGAATC TTCACTACCT CTGCCTCAGC CATCACCGTT CATGCCCAGG 
CGGCATATTC ATGGAGAGAG CTCCAGCAAA AAGCTACAAA GATCTCAGCG AATCAGTGAT 
AGTGACATCT CAGATTATGA GGTTGATGAT GGTATTGGAG TAGTGCCTCC AGTGGGTTAT 
AGAGCTAGTG CTAGAGAGAG TAAAGCAACC ACGTTAACAG TGCCAGAGCA ACAAAGAACT 
ACACATCACC GCTCACGTTC CGTGTCTCCT CATCGCGGCG ATGATCAGGG AAGGCCTCGT 
TCACGTTTAC CAAATGTGCC ATTACAGAGG AGCTTAGATG AAATTCATCC AACACGAAGG 
TCACGTTCTC CAACCCGACA CCATGATGCC TCCCGAAGCC CGGCCGATCA CAGATCCAGA 
CATGTGGAAA GTCAATATTC GTCAGAGCCA GACAGTGAGC TTCTCATGCT GCCCAGAGCA 
AAACGAGGAC GAAGTGCAGA AAGCCTACAC ATGACCAGTG AACTGCAGCC CTCTCTTGAC 
AGGGCTAGGA GTGCTAGTAC CAACTGCTTG AGACCAGATA CTAGTTTGCA TTCACCAGAA 
CGAGAAAGAA CGCACCGACA AGGAAGCCCA ACCCAGTCTC CTCCAGCAGA CACATCCTTC 
GGCAGTCGCC GTGGAAGACA GCTCCCACAG GTGCCAGTTC GAAGCGGCAG TATAGAACAA 
GCAAGCTTAG TAGTGGAGGA GCGAACGAGA CAGATGAAAA TGAAAGTTCA CCGATTTAAG 
CAGACAACAG GGTCTGGGTC TAGTCAAGAA CTTGACCACG AGCAATACTC CAAGAAGTCA 
CGTTACATTA TGGAGTACAA CATACATAAA GATCAGTACA GAAGCTGTGA TAACGCGTCT 
GCCAAGTCTT CAGATAGTGA TGTCAGTGAT GTGTCCGCCA TTTCCAGAGC CAGCAGTACC 
TCACGCCTCA GCAGCACAAG CTTTATGTCA GAGCAGTCTG AGCGCCCCAG GGGTAGGATC 
AGTTCATTTA CCCCCAAAAT GCAAGGCAGA CGGATGGGGA CTTCAGGAAG AGCCATCATC 
AAGAGCACCA GTGTAAGTGG AGAGATATAT ACACTGGAAC GTAATGACGG TAGCCAGTCG 
GACACGGCCG TGGGTACCGT CGGAGCCGGT GGAAAGAAAC GAAGATCCAG CCTGAGCGCC 
AAAGTGGTAG CCATTGTGTC TCGAAGAAGC AGGAGCACGT CACAGCTCAG CCAGACAGAG 
TCGGGCCACA AGAAGTTGAA AAGCACCATC CAGAGGAGTA CGGAAACAGG AATGGCAGCT 
GAAATGCGGA AGATGGTGAG ACAGCCGAGC CGGGAGTCCA CGGATGGCAG CATCAACAGT 
TATAGCTCGG AAGGAAACTT GATATTTCCT GGAGTTCGAG TAGGACCCGA CAGTCAGTTC 
AGTGATTTCC TTGATGGGTT GGGACCAGCG CAGCTCGTTG GCCGTCAGAC GCTCGCCACC 
CCGGCCATGG GCGATATCCA AATCGGGATG GAGGATAAGA AGGGTCAGTT GGAGGTTGAG 
GTTATCAGAG CCCGGAGCCT TACACAAAAA CCTGGTTCCA AATCTACACC CGCTCCCTAT 
GTGAAAGTAT ATCTTTTGGA AAATGGAGCC TGTATTGCCA AAAAGAAGAC AAGAATTGCA 
CGGAAAACTC TCGATCCTTT GTATCAGCAG TCCCTGGTTT TTGATGAAAG TCCACAGGGT 
AAAGTTCTTC AGGTGATTGT CTGGGGTGAC TATGGAAGAA TGGACCACAA ATGCTTTATG 
GGTGTGGCTC AAATCTTGTT GGAAGAACTT GATCTATCCA GCATGGTGAT TGGATGGTAT 
AAATTGTTCC CTCCGTCCTC ACTGGTGGAT CCCACTCTCG CTCCCCTGAC CCGCCGGGCT 
TCCCAATCAT CTCTGGAAAG TTCGTCCGGG CCTCCCTGCA TCCGGTCATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017452155.1
CDS	307..4917
Translation	MSSAVGPRGPRPPTVPPPMQELPDLSHLTEEERNIIMAVMDRQKEEEEKEEAMLKCVVRDMAKPAACKTPRNAESQPHQPPLNIFRCVCVPRKPSSEEGGPERDWRLHQQFESYKEQVRKIGEEARRYQGEHKDDAPTCGICHKTKFADGCGHLCSYCRTKFCARCGGRVSLRSNNEDKVVMWVCNLCRKQQEILTKSGAWFFGSGPQQPSQDGTLSDTATGAGSEVPREKKARLQERSRSQTPLSTAAVSSQDTATPGAPLDRNKGAEPSQQALGPEQKQASRSRSEPPRERKKAPGLSEQNGKGGQKSERKRVPKSVVQPGEGIADERERKERRETRRLEKGRSQDYSDRPEKRDNGRVAEDQKQRKEEEYQTRYRSDPNLARYPVKAPPEEQQMRMHARVSRARHERRHSDVALPHTEAAAAAPAEATAGKRAPATARVSPPESPRARAPAAQPPTEHGPPPPRPVPGPAEPPEPRVPEPLRKQGRLDPASAVLLRKAKREKAESMLRNDSLSSDQSESVRPSPPKPHRPKRGGKRRQMSVSSSEEEGVSTPEYTSCEDVELESESVSEKGDLDYYWLDPATWHSRETSPISSHPVTWQPSKEGDRLIGRVILNKRTTMPKESGALLGLKVVGGKMTDLGRLGAFITKVKKGSLADVVGHLRAGDEVLEWNGKPLPGATNEEVYNIILESKSEPQVEIIVSRPIGDIPRIPESSHPPLESSSSSFESQKMERPSISVISPTSPGALKDAPQVLPGQLSVKLWYDKVGHQLIVNVLQATDLPPRVDGRPRNPYVKMYFLPDRSDKSKRRTKTVKKLLEPKWNQTFVYSHVHRRDFRERMLEITVWDQPRVQDEESEFLGEILIELETALLDDEPHWYKLQTHDESSLPLPQPSPFMPRRHIHGESSSKKLQRSQRISDSDISDYEVDDGIGVVPPVGYRASARESKATTLTVPEQQRTTHHRSRSVSPHRGDDQGRPRSRLPNVPLQRSLDEIHPTRRSRSPTRHHDASRSPADHRSRHVESQYSSEPDSELLMLPRAKRGRSAESLHMTSELQPSLDRARSASTNCLRPDTSLHSPERERTHRQGSPTQSPPADTSFGSRRGRQLPQVPVRSGSIEQASLVVEERTRQMKMKVHRFKQTTGSGSSQELDHEQYSKKSRYIMEYNIHKDQYRSCDNASAKSSDSDVSDVSAISRASSTSRLSSTSFMSEQSERPRGRISSFTPKMQGRRMGTSGRAIIKSTSVSGEIYTLERNDGSQSDTAVGTVGAGGKKRRSSLSAKVVAIVSRRSRSTSQLSQTESGHKKLKSTIQRSTETGMAAEMRKMVRQPSRESTDGSINSYSSEGNLIFPGVRVGPDSQFSDFLDGLGPAQLVGRQTLATPAMGDIQIGMEDKKGQLEVEVIRARSLTQKPGSKSTPAPYVKVYLLENGACIAKKKTRIARKTLDPLYQQSLVFDESPQGKVLQVIVWGDYGRMDHKCFMGVAQILLEELDLSSMVIGWYKLFPPSSLVDPTLAPLTRRASQSSLESSSGPPCIRS

Target ORF information:

RefSeq Version	XM_017596666.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus regulating synaptic membrane exocytosis 1 (Rims1), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017596666.1

ORF Insert Sequence:

ATGTCCTCGG CCGTGGGGCC CCGAGGTCCT CGCCCACCCA CGGTGCCTCC CCCTATGCAA 
GAACTGCCCG ACCTGAGCCA CCTGACCGAG GAGGAGAGGA ACATTATCAT GGCAGTGATG 
GACCGGCAGA AGGAAGAGGA GGAAAAAGAA GAGGCCATGC TCAAGTGTGT TGTCAGGGAC 
ATGGCGAAGC CTGCTGCCTG CAAAACACCA AGAAATGCTG AAAGCCAGCC CCATCAACCA 
CCACTGAACA TTTTCAGATG TGTCTGTGTT CCCAGAAAGC CAAGCAGCGA AGAGGGAGGC 
CCAGAAAGAG ACTGGAGATT GCATCAACAG TTTGAAAGCT ACAAGGAGCA AGTGAGAAAA 
ATCGGAGAGG AAGCGAGGCG TTACCAGGGC GAGCACAAGG ATGATGCCCC GACGTGTGGA 
ATCTGTCATA AGACAAAGTT TGCTGATGGA TGTGGCCATC TCTGCTCCTA TTGTCGCACC 
AAGTTCTGTG CACGCTGCGG AGGCCGTGTG TCTCTGCGAT CGAACAATGA GGACAAAGTG 
GTTATGTGGG TATGCAATTT ATGTCGAAAG CAACAAGAAA TCTTAACGAA ATCTGGAGCG 
TGGTTCTTTG GAAGTGGCCC TCAGCAGCCT AGTCAAGATG GGACTCTGAG TGACACGGCC 
ACAGGTGCTG GATCTGAGGT GCCAAGAGAA AAGAAAGCAA GGCTCCAAGA GCGATCAAGG 
TCTCAGACGC CCTTGAGTAC AGCAGCTGTC TCTTCCCAAG ACACTGCTAC CCCCGGTGCA 
CCGTTGGACA GGAACAAAGG GGCTGAGCCC TCACAGCAAG CCTTGGGTCC TGAACAGAAG 
CAGGCATCAA GATCAAGAAG CGAGCCACCG AGGGAAAGGA AGAAGGCTCC AGGGCTTTCA 
GAGCAGAATG GCAAGGGAGG CCAGAAGAGC GAGCGCAAAC GTGTCCCCAA GTCTGTGGTG 
CAACCCGGGG AAGGGATCGC GGATGAGAGG GAGAGGAAAG AGAGGCGGGA AACCCGCAGG 
TTGGAGAAAG GGCGCTCCCA GGACTACTCA GACCGGCCTG AGAAACGCGA CAATGGCAGG 
GTGGCGGAAG ACCAGAAGCA GAGGAAGGAG GAGGAGTACC AGACTAGGTA CCGCAGCGAC 
CCTAACCTGG CTCGCTACCC GGTGAAGGCG CCGCCAGAGG AGCAGCAGAT GCGCATGCAC 
GCCCGGGTGT CCCGAGCGAG GCACGAGCGG CGCCACAGCG ACGTGGCGCT CCCGCACACC 
GAGGCAGCTG CCGCCGCGCC GGCTGAGGCC ACGGCGGGCA AGCGCGCGCC GGCCACCGCC 
AGGGTCTCTC CCCCGGAGTC CCCGCGCGCA CGCGCGCCCG CCGCCCAGCC TCCCACCGAG 
CACGGGCCAC CGCCGCCGCG GCCAGTCCCG GGTCCCGCAG AGCCACCCGA GCCGCGCGTC 
CCCGAGCCGC TCCGTAAGCA GGGCCGCCTG GACCCGGCCT CGGCCGTGCT TCTGCGCAAG 
GCCAAGCGCG AGAAGGCGGA GAGCATGCTG CGGAACGACT CGCTGAGCTC CGATCAGTCC 
GAGTCCGTGC GGCCATCCCC GCCCAAGCCT CACCGGCCCA AGCGGGGAGG CAAGAGACGT 
CAGATGTCGG TGAGCAGCTC GGAGGAGGAG GGCGTGTCCA CACCGGAGTA CACGAGCTGC 
GAGGACGTGG AGCTGGAGAG CGAGAGCGTG AGCGAGAAAG GTGACTTGGA TTACTACTGG 
TTGGATCCCG CCACGTGGCA CAGCAGGGAA ACGTCGCCTA TCAGTTCGCA TCCTGTAACG 
TGGCAGCCGT CTAAAGAGGG AGATCGACTA ATCGGCCGTG TTATTCTTAA CAAAAGAACA 
ACCATGCCCA AAGAATCAGG TGCATTATTG GGTCTGAAGG TGGTTGGAGG AAAAATGACG 
GACTTAGGGC GCCTTGGTGC TTTCATCACC AAAGTAAAGA AGGGCAGCCT GGCAGACGTC 
GTCGGACACC TAAGAGCAGG GGACGAAGTC CTAGAGTGGA ATGGTAAACC CCTGCCGGGA 
GCAACAAACG AAGAAGTTTA CAACATTATC TTAGAATCAA AATCAGAACC TCAAGTTGAG 
ATTATTGTTT CAAGGCCTAT TGGTGACATC CCCAGGATCC CTGAGAGTTC CCATCCTCCC 
CTGGAGTCCA GTTCAAGTTC CTTTGAATCT CAGAAAATGG AAAGGCCTTC TATTTCTGTT 
ATTTCTCCAA CCAGCCCTGG AGCTCTGAAA GATGCCCCAC AAGTCTTACC AGGGCAACTC 
TCAGTGAAGC TATGGTATGA TAAAGTGGGG CACCAGCTGA TTGTAAATGT TCTACAAGCA 
ACAGATCTAC CCCCTAGAGT AGATGGCCGT CCCAGGAATC CCTATGTAAA AATGTATTTT 
CTTCCAGATA GAAGCGACAA AAGTAAAAGG AGAACCAAAA CAGTAAAGAA ACTTCTAGAG 
CCAAAATGGA ACCAGACATT TGTCTACTCA CACGTACATC GTAGAGATTT TCGAGAGCGA 
ATGTTAGAGA TTACCGTGTG GGACCAGCCG AGAGTACAGG ACGAAGAGAG TGAATTTCTT 
GGAGAGATCC TCATAGAGTT GGAAACAGCG CTTTTAGATG ATGAGCCCCA TTGGTATAAA 
CTCCAGACAC ATGACGAATC TTCACTACCT CTGCCTCAGC CATCACCGTT CATGCCCAGG 
CGGCATATTC ATGGAGAGAG CTCCAGCAAA AAGCTACAAA GATCTCAGCG AATCAGTGAT 
AGTGACATCT CAGATTATGA GGTTGATGAT GGTATTGGAG TAGTGCCTCC AGTGGGTTAT 
AGAGCTAGTG CTAGAGAGAG TAAAGCAACC ACGTTAACAG TGCCAGAGCA ACAAAGAACT 
ACACATCACC GCTCACGTTC CGTGTCTCCT CATCGCGGCG ATGATCAGGG AAGGCCTCGT 
TCACGTTTAC CAAATGTGCC ATTACAGAGG AGCTTAGATG AAATTCATCC AACACGAAGG 
TCACGTTCTC CAACCCGACA CCATGATGCC TCCCGAAGCC CGGCCGATCA CAGATCCAGA 
CATGTGGAAA GTCAATATTC GTCAGAGCCA GACAGTGAGC TTCTCATGCT GCCCAGAGCA 
AAACGAGGAC GAAGTGCAGA AAGCCTACAC ATGACCAGTG AACTGCAGCC CTCTCTTGAC 
AGGGCTAGGA GTGCTAGTAC CAACTGCTTG AGACCAGATA CTAGTTTGCA TTCACCAGAA 
CGAGAAAGAA CGCACCGACA AGGAAGCCCA ACCCAGTCTC CTCCAGCAGA CACATCCTTC 
GGCAGTCGCC GTGGAAGACA GCTCCCACAG GTGCCAGTTC GAAGCGGCAG TATAGAACAA 
GCAAGCTTAG TAGTGGAGGA GCGAACGAGA CAGATGAAAA TGAAAGTTCA CCGATTTAAG 
CAGACAACAG GGTCTGGGTC TAGTCAAGAA CTTGACCACG AGCAATACTC CAAGAAGTCA 
CGTTACATTA TGGAGTACAA CATACATAAA GATCAGTACA GAAGCTGTGA TAACGCGTCT 
GCCAAGTCTT CAGATAGTGA TGTCAGTGAT GTGTCCGCCA TTTCCAGAGC CAGCAGTACC 
TCACGCCTCA GCAGCACAAG CTTTATGTCA GAGCAGTCTG AGCGCCCCAG GGGTAGGATC 
AGTTCATTTA CCCCCAAAAT GCAAGGCAGA CGGATGGGGA CTTCAGGAAG AGCCATCATC 
AAGAGCACCA GTGTAAGTGG AGAGATATAT ACACTGGAAC GTAATGACGG TAGCCAGTCG 
GACACGGCCG TGGGTACCGT CGGAGCCGGT GGAAAGAAAC GAAGATCCAG CCTGAGCGCC 
AAAGTGGTAG CCATTGTGTC TCGAAGAAGC AGGAGCACGT CACAGCTCAG CCAGACAGAG 
TCGGGCCACA AGAAGTTGAA AAGCACCATC CAGAGGAGTA CGGAAACAGG AATGGCAGCT 
GAAATGCGGA AGATGGTGAG ACAGCCGAGC CGGGAGTCCA CGGATGGCAG CATCAACAGT 
TATAGCTCGG AAGGAAACTT GATATTTCCT GGAGTTCGAG TAGGACCCGA CAGTCAGTTC 
AGTGATTTCC TTGATGGGTT GGGACCAGCG CAGCTCGTTG GCCGTCAGAC GCTCGCCACC 
CCGGCCATGG GCGATATCCA AATCGGGATG GAGGATAAGA AGGGTCAGTT GGAGGTTGAG 
GTTATCAGAG CCCGGAGCCT TACACAAAAA CCTGGTTCCA AATCTACACC CGCTCCCTAT 
GTGAAAGTAT ATCTTTTGGA AAATGGAGCC TGTATTGCCA AAAAGAAGAC AAGAATTGCA 
CGGAAAACTC TCGATCCTTT GTATCAGCAG TCCCTGGTTT TTGATGAAAG TCCACAGGGT 
AAAGTTCTTC AGGTGATTGT CTGGGGTGAC TATGGAAGAA TGGACCACAA ATGCTTTATG 
GGTGTGGCTC AAATCTTGTT GGAAGAACTT GATCTATCCA GCATGGTGAT TGGATGGTAT 
AAATTGTTCC CTCCGTCCTC ACTGGTGGAT CCCACTCTCG CTCCCCTGAC CCGCCGGGCT 
TCCCAATCAT CTCTGGAAAG TTCGTCCGGG CCTCCCTGCA TCCGGTCATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rims1 ( XM_017596667.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017452156.1 Rattus norvegicus regulating synaptic membrane exocytosis 1 (Rims1), transcript variant X14, mRNA.

Price & Availability↑

CloneID	ORa62884
Clone ID Related Accession (Same CDS sequence)	XM_017596667.1
Accession Version	XM_017596667.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4590bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	regulating synaptic membrane exocytosis protein 1 isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005108.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCTCGG CCGTGGGGCC CCGAGGTCCT CGCCCACCCA CGGTGCCTCC CCCTATGCAA 
GAACTGCCCG ACCTGAGCCA CCTGACCGAG GAGGAGAGGA ACATTATCAT GGCAGTGATG 
GACCGGCAGA AGGAAGAGGA GGAAAAAGAA GAGGCCATGC TCAAGTGTGT TGTCAGGGAC 
ATGGCGAAGC CTGCTGCCTG CAAAACACCA AGAAATGCTG AAAGCCAGCC CCATCAACCA 
CCACTGAACA TTTTCAGATG TGTCTGTGTT CCCAGAAAGC CAAGCAGCGA AGAGGGAGGC 
CCAGAAAGAG ACTGGAGATT GCATCAACAG TTTGAAAGCT ACAAGGAGCA AGTGAGAAAA 
ATCGGAGAGG AAGCGAGGCG TTACCAGGGC GAGCACAAGG ATGATGCCCC GACGTGTGGA 
ATCTGTCATA AGACAAAGTT TGCTGATGGA TGTGGCCATC TCTGCTCCTA TTGTCGCACC 
AAGTTCTGTG CACGCTGCGG AGGCCGTGTG TCTCTGCGAT CGAACAATGA GGACAAAGTG 
GTTATGTGGG TATGCAATTT ATGTCGAAAG CAACAAGAAA TCTTAACGAA ATCTGGAGCG 
TGGTTCTTTG GAAGTGGCCC TCAGCAGCCT AGTCAAGATG GGACTCTGAG TGACACGGCC 
ACAGGTGCTG GATCTGAGGT GCCAAGAGAA AAGAAAGCAA GGCTCCAAGA GCGATCAAGG 
TCTCAGACGC CCTTGAGTAC AGCAGCTGTC TCTTCCCAAG ACACTGCTAC CCCCGGTGCA 
CCGTTGGACA GGAACAAAGG GGCTGAGCCC TCACAGCAAG CCTTGGGTCC TGAACAGAAG 
CAGGCATCAA GATCAAGAAG CGAGCCACCG AGGGAAAGGA AGAAGGCTCC AGGGCTTTCA 
GAGCAGAATG GCAAGGGAGG CCAGAAGAGC GAGCGCAAAC GTGTCCCCAA GTCTGTGGTG 
CAACCCGGGG AAGGGATCGC GGATGAGAGG GAGAGGAAAG AGAGGCGGGA AACCCGCAGG 
TTGGAGAAAG GGCGCTCCCA GGACTACTCA GACCGGCCTG AGAAACGCGA CAATGGCAGG 
GTGGCGGAAG ACCAGAAGCA GAGGAAGGAG GAGGAGTACC AGACTAGGTA CCGCAGCGAC 
CCTAACCTGG CTCGCTACCC GGTGAAGGCG CCGCCAGAGG AGCAGCAGAT GCGCATGCAC 
GCCCGGGTGT CCCGAGCGAG GCACGAGCGG CGCCACAGCG ACGTGGCGCT CCCGCACACC 
GAGGCAGCTG CCGCCGCGCC GGCTGAGGCC ACGGCGGGCA AGCGCGCGCC GGCCACCGCC 
AGGGTCTCTC CCCCGGAGTC CCCGCGCGCA CGCGCGCCCG CCGCCCAGCC TCCCACCGAG 
CACGGGCCAC CGCCGCCGCG GCCAGTCCCG GGTCCCGCAG AGCCACCCGA GCCGCGCGTC 
CCCGAGCCGC TCCGTAAGCA GGGCCGCCTG GACCCGGCCT CGGCCGTGCT TCTGCGCAAG 
GCCAAGCGCG AGAAGGCGGA GAGCATGCTG CGGAACGACT CGCTGAGCTC CGATCAGTCC 
GAGTCCGTGC GGCCATCCCC GCCCAAGCCT CACCGGCCCA AGCGGGGAGG CAAGAGACGT 
CAGATGTCGG TGAGCAGCTC GGAGGAGGAG GGCGTGTCCA CACCGGAGTA CACGAGCTGC 
GAGGACGTGG AGCTGGAGAG CGAGAGCGTG AGCGAGAAAG GTGACTTGGA TTACTACTGG 
TTGGATCCCG CCACGTGGCA CAGCAGGGAA ACGTCGCCTA TCAGTTCGCA TCCTGTAACG 
TGGCAGCCGT CTAAAGAGGG AGATCGACTA ATCGGCCGTG TTATTCTTAA CAAAAGAACA 
ACCATGCCCA AAGAATCAGG TGCATTATTG GGTCTGAAGG TGGTTGGAGG AAAAATGACG 
GACTTAGGGC GCCTTGGTGC TTTCATCACC AAAGTAAAGA AGGGCAGCCT GGCAGACGTC 
GTCGGACACC TAAGAGCAGG GGACGAAGTC CTAGAGTGGA ATGGTAAACC CCTGCCGGGA 
GCAACAAACG AAGAAGTTTA CAACATTATC TTAGAATCAA AATCAGAACC TCAAGTTGAG 
ATTATTGTTT CAAGGCCTAT TGGTGACATC CCCAGGATCC CTGAGAGTTC CCATCCTCCC 
CTGGAGTCCA GTTCAAGTTC CTTTGAATCT CAGAAAATGG AAAGGCCTTC TATTTCTGTT 
ATTTCTCCAA CCAGCCCTGG AGCTCTGAAA GATGCCCCAC AAGTCTTACC AGGGCAACTC 
TCAGTGAAGC TATGGTATGA TAAAGTGGGG CACCAGCTGA TTGTAAATGT TCTACAAGCA 
ACAGATCTAC CCCCTAGAGT AGATGGCCGT CCCAGGAATC CCTATGTAAA AATGTATTTT 
CTTCCAGATA GAAGCGACAA AAGTAAAAGG AGAACCAAAA CAGTAAAGAA ACTTCTAGAG 
CCAAAATGGA ACCAGACATT TGTCTACTCA CACGTACATC GTAGAGATTT TCGAGAGCGA 
ATGTTAGAGA TTACCGTGTG GGACCAGCCG AGAGTACAGG ACGAAGAGAG TGAATTTCTT 
GGAGAGATCC TCATAGAGTT GGAAACAGCG CTTTTAGATG ATGAGCCCCA TTGGTATAAA 
CTCCAGACAC ATGACGAATC TTCACTACCT CTGCCTCAGC CATCACCGTT CATGCCCAGG 
CGGCATATTC ATGGAGAGAG CTCCAGCAAA AAGCTACAAA GATCTCAGCG AATCAGTGAT 
AGTGACATCT CAGATTATGA GGTTGATGAT GGTATTGGAG TAGTGCCTCC AGTGGGTTAT 
AGAGCTAGTG CTAGAGAGAG TAAAGCAACC ACGTTAACAG TGCCAGAGCA ACAAAGAACT 
ACACATCACC GCTCACGTTC CGTGTCTCCT CATCGCGGCG ATGATCAGGG AAGGCCTCGT 
TCACGTTTAC CAAATGTGCC ATTACAGAGG AGCTTAGATG AAATTCATCC AACACGAAGG 
TCACGTTCTC CAACCCGACA CCATGATGCC TCCCGAAGCC CGGCCGATCA CAGATCCAGA 
CATGTGGAAA GTCAATATTC GTCAGAGCCA GACAGTGAGC TTCTCATGCT GCCCAGAGCA 
AAACGAGGAC GAAGTGCAGA AAGCCTACAC ATGACCAGTG AACTGCAGCC CTCTCTTGAC 
AGGGCTAGGA GTGCTAGTAC CAACTGCTTG AGACCAGATA CTAGTTTGCA TTCACCAGAA 
CGAGAAAGAA CGCACCGACA AGGAAGCCCA ACCCAGTCTC CTCCAGCAGA CACATCCTTC 
GGCAGTCGCC GTGGAAGACA GCTCCCACAG GTGCCAGTTC GAAGCGGCAG TATAGAACAA 
GCAAGCTTAG TAGTGGAGGA GCGAACGAGA CAGATGAAAA TGAAAGTTCA CCGATTTAAG 
CAGACAACAG GGTCTGGGTC TAGTCAAGAA CTTGACCACG AGCAATACTC CAAGTACAAC 
ATACATAAAG ATCAGTACAG AAGCTGTGAT AACGCGTCTG CCAAGTCTTC AGATAGTGAT 
GTCAGTGATG TGTCCGCCAT TTCCAGAGCC AGCAGTACCT CACGCCTCAG CAGCACAAGC 
TTTATGTCAG AGCAGTCTGA GCGCCCCAGG GGTAGGATCA GTTCATTTAC CCCCAAAATG 
CAAGGCAGAC GGATGGGGAC TTCAGGAAGA GCCATCATCA AGAGCACCAG TGTAAGTGGA 
GAGATATATA CACTGGAACG TAATGACGGT AGCCAGTCGG ACACGGCCGT GGGTACCGTC 
GGAGCCGGTG GAAAGAAACG AAGATCCAGC CTGAGCGCCA AAGTGGTAGC CATTGTGTCT 
CGAAGAAGCA GGAGCACGTC ACAGCTCAGC CAGACAGAGT CGGGCCACAA GAAGTTGAAA 
AGCACCATCC AGAGGAGTAC GGAAACAGGA ATGGCAGCTG AAATGCGGAA GATGGTGAGA 
CAGCCGAGCC GGGAGTCCAC GGATGGCAGC ATCAACAGTT ATAGCTCGGA AGGAAACTTG 
ATATTTCCTG GAGTTCGAGT AGGACCCGAC AGTCAGTTCA GTGATTTCCT TGATGGGTTG 
GGACCAGCGC AGCTCGTTGG CCGTCAGACG CTCGCCACCC CGGCCATGGG CGATATCCAA 
ATCGGGATGG AGGATAAGAA GGGTCAGTTG GAGGTTGAGG TTATCAGAGC CCGGAGCCTT 
ACACAAAAAC CTGGTTCCAA ATCTACACCC GCTCCCTATG TGAAAGTATA TCTTTTGGAA 
AATGGAGCCT GTATTGCCAA AAAGAAGACA AGAATTGCAC GGAAAACTCT CGATCCTTTG 
TATCAGCAGT CCCTGGTTTT TGATGAAAGT CCACAGGGTA AAGTTCTTCA GGTGATTGTC 
TGGGGTGACT ATGGAAGAAT GGACCACAAA TGCTTTATGG GTGTGGCTCA AATCTTGTTG 
GAAGAACTTG ATCTATCCAG CATGGTGATT GGATGGTATA AATTGTTCCC TCCGTCCTCA 
CTGGTGGATC CCACTCTCGC TCCCCTGACC CGCCGGGCTT CCCAATCATC TCTGGAAAGT 
TCGTCCGGGC CTCCCTGCAT CCGGTCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017452156.1
CDS	306..4895
Translation	MSSAVGPRGPRPPTVPPPMQELPDLSHLTEEERNIIMAVMDRQKEEEEKEEAMLKCVVRDMAKPAACKTPRNAESQPHQPPLNIFRCVCVPRKPSSEEGGPERDWRLHQQFESYKEQVRKIGEEARRYQGEHKDDAPTCGICHKTKFADGCGHLCSYCRTKFCARCGGRVSLRSNNEDKVVMWVCNLCRKQQEILTKSGAWFFGSGPQQPSQDGTLSDTATGAGSEVPREKKARLQERSRSQTPLSTAAVSSQDTATPGAPLDRNKGAEPSQQALGPEQKQASRSRSEPPRERKKAPGLSEQNGKGGQKSERKRVPKSVVQPGEGIADERERKERRETRRLEKGRSQDYSDRPEKRDNGRVAEDQKQRKEEEYQTRYRSDPNLARYPVKAPPEEQQMRMHARVSRARHERRHSDVALPHTEAAAAAPAEATAGKRAPATARVSPPESPRARAPAAQPPTEHGPPPPRPVPGPAEPPEPRVPEPLRKQGRLDPASAVLLRKAKREKAESMLRNDSLSSDQSESVRPSPPKPHRPKRGGKRRQMSVSSSEEEGVSTPEYTSCEDVELESESVSEKGDLDYYWLDPATWHSRETSPISSHPVTWQPSKEGDRLIGRVILNKRTTMPKESGALLGLKVVGGKMTDLGRLGAFITKVKKGSLADVVGHLRAGDEVLEWNGKPLPGATNEEVYNIILESKSEPQVEIIVSRPIGDIPRIPESSHPPLESSSSSFESQKMERPSISVISPTSPGALKDAPQVLPGQLSVKLWYDKVGHQLIVNVLQATDLPPRVDGRPRNPYVKMYFLPDRSDKSKRRTKTVKKLLEPKWNQTFVYSHVHRRDFRERMLEITVWDQPRVQDEESEFLGEILIELETALLDDEPHWYKLQTHDESSLPLPQPSPFMPRRHIHGESSSKKLQRSQRISDSDISDYEVDDGIGVVPPVGYRASARESKATTLTVPEQQRTTHHRSRSVSPHRGDDQGRPRSRLPNVPLQRSLDEIHPTRRSRSPTRHHDASRSPADHRSRHVESQYSSEPDSELLMLPRAKRGRSAESLHMTSELQPSLDRARSASTNCLRPDTSLHSPERERTHRQGSPTQSPPADTSFGSRRGRQLPQVPVRSGSIEQASLVVEERTRQMKMKVHRFKQTTGSGSSQELDHEQYSKYNIHKDQYRSCDNASAKSSDSDVSDVSAISRASSTSRLSSTSFMSEQSERPRGRISSFTPKMQGRRMGTSGRAIIKSTSVSGEIYTLERNDGSQSDTAVGTVGAGGKKRRSSLSAKVVAIVSRRSRSTSQLSQTESGHKKLKSTIQRSTETGMAAEMRKMVRQPSRESTDGSINSYSSEGNLIFPGVRVGPDSQFSDFLDGLGPAQLVGRQTLATPAMGDIQIGMEDKKGQLEVEVIRARSLTQKPGSKSTPAPYVKVYLLENGACIAKKKTRIARKTLDPLYQQSLVFDESPQGKVLQVIVWGDYGRMDHKCFMGVAQILLEELDLSSMVIGWYKLFPPSSLVDPTLAPLTRRASQSSLESSSGPPCIRS

Target ORF information:

RefSeq Version	XM_017596667.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus regulating synaptic membrane exocytosis 1 (Rims1), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017596667.1

ORF Insert Sequence:

ATGTCCTCGG CCGTGGGGCC CCGAGGTCCT CGCCCACCCA CGGTGCCTCC CCCTATGCAA 
GAACTGCCCG ACCTGAGCCA CCTGACCGAG GAGGAGAGGA ACATTATCAT GGCAGTGATG 
GACCGGCAGA AGGAAGAGGA GGAAAAAGAA GAGGCCATGC TCAAGTGTGT TGTCAGGGAC 
ATGGCGAAGC CTGCTGCCTG CAAAACACCA AGAAATGCTG AAAGCCAGCC CCATCAACCA 
CCACTGAACA TTTTCAGATG TGTCTGTGTT CCCAGAAAGC CAAGCAGCGA AGAGGGAGGC 
CCAGAAAGAG ACTGGAGATT GCATCAACAG TTTGAAAGCT ACAAGGAGCA AGTGAGAAAA 
ATCGGAGAGG AAGCGAGGCG TTACCAGGGC GAGCACAAGG ATGATGCCCC GACGTGTGGA 
ATCTGTCATA AGACAAAGTT TGCTGATGGA TGTGGCCATC TCTGCTCCTA TTGTCGCACC 
AAGTTCTGTG CACGCTGCGG AGGCCGTGTG TCTCTGCGAT CGAACAATGA GGACAAAGTG 
GTTATGTGGG TATGCAATTT ATGTCGAAAG CAACAAGAAA TCTTAACGAA ATCTGGAGCG 
TGGTTCTTTG GAAGTGGCCC TCAGCAGCCT AGTCAAGATG GGACTCTGAG TGACACGGCC 
ACAGGTGCTG GATCTGAGGT GCCAAGAGAA AAGAAAGCAA GGCTCCAAGA GCGATCAAGG 
TCTCAGACGC CCTTGAGTAC AGCAGCTGTC TCTTCCCAAG ACACTGCTAC CCCCGGTGCA 
CCGTTGGACA GGAACAAAGG GGCTGAGCCC TCACAGCAAG CCTTGGGTCC TGAACAGAAG 
CAGGCATCAA GATCAAGAAG CGAGCCACCG AGGGAAAGGA AGAAGGCTCC AGGGCTTTCA 
GAGCAGAATG GCAAGGGAGG CCAGAAGAGC GAGCGCAAAC GTGTCCCCAA GTCTGTGGTG 
CAACCCGGGG AAGGGATCGC GGATGAGAGG GAGAGGAAAG AGAGGCGGGA AACCCGCAGG 
TTGGAGAAAG GGCGCTCCCA GGACTACTCA GACCGGCCTG AGAAACGCGA CAATGGCAGG 
GTGGCGGAAG ACCAGAAGCA GAGGAAGGAG GAGGAGTACC AGACTAGGTA CCGCAGCGAC 
CCTAACCTGG CTCGCTACCC GGTGAAGGCG CCGCCAGAGG AGCAGCAGAT GCGCATGCAC 
GCCCGGGTGT CCCGAGCGAG GCACGAGCGG CGCCACAGCG ACGTGGCGCT CCCGCACACC 
GAGGCAGCTG CCGCCGCGCC GGCTGAGGCC ACGGCGGGCA AGCGCGCGCC GGCCACCGCC 
AGGGTCTCTC CCCCGGAGTC CCCGCGCGCA CGCGCGCCCG CCGCCCAGCC TCCCACCGAG 
CACGGGCCAC CGCCGCCGCG GCCAGTCCCG GGTCCCGCAG AGCCACCCGA GCCGCGCGTC 
CCCGAGCCGC TCCGTAAGCA GGGCCGCCTG GACCCGGCCT CGGCCGTGCT TCTGCGCAAG 
GCCAAGCGCG AGAAGGCGGA GAGCATGCTG CGGAACGACT CGCTGAGCTC CGATCAGTCC 
GAGTCCGTGC GGCCATCCCC GCCCAAGCCT CACCGGCCCA AGCGGGGAGG CAAGAGACGT 
CAGATGTCGG TGAGCAGCTC GGAGGAGGAG GGCGTGTCCA CACCGGAGTA CACGAGCTGC 
GAGGACGTGG AGCTGGAGAG CGAGAGCGTG AGCGAGAAAG GTGACTTGGA TTACTACTGG 
TTGGATCCCG CCACGTGGCA CAGCAGGGAA ACGTCGCCTA TCAGTTCGCA TCCTGTAACG 
TGGCAGCCGT CTAAAGAGGG AGATCGACTA ATCGGCCGTG TTATTCTTAA CAAAAGAACA 
ACCATGCCCA AAGAATCAGG TGCATTATTG GGTCTGAAGG TGGTTGGAGG AAAAATGACG 
GACTTAGGGC GCCTTGGTGC TTTCATCACC AAAGTAAAGA AGGGCAGCCT GGCAGACGTC 
GTCGGACACC TAAGAGCAGG GGACGAAGTC CTAGAGTGGA ATGGTAAACC CCTGCCGGGA 
GCAACAAACG AAGAAGTTTA CAACATTATC TTAGAATCAA AATCAGAACC TCAAGTTGAG 
ATTATTGTTT CAAGGCCTAT TGGTGACATC CCCAGGATCC CTGAGAGTTC CCATCCTCCC 
CTGGAGTCCA GTTCAAGTTC CTTTGAATCT CAGAAAATGG AAAGGCCTTC TATTTCTGTT 
ATTTCTCCAA CCAGCCCTGG AGCTCTGAAA GATGCCCCAC AAGTCTTACC AGGGCAACTC 
TCAGTGAAGC TATGGTATGA TAAAGTGGGG CACCAGCTGA TTGTAAATGT TCTACAAGCA 
ACAGATCTAC CCCCTAGAGT AGATGGCCGT CCCAGGAATC CCTATGTAAA AATGTATTTT 
CTTCCAGATA GAAGCGACAA AAGTAAAAGG AGAACCAAAA CAGTAAAGAA ACTTCTAGAG 
CCAAAATGGA ACCAGACATT TGTCTACTCA CACGTACATC GTAGAGATTT TCGAGAGCGA 
ATGTTAGAGA TTACCGTGTG GGACCAGCCG AGAGTACAGG ACGAAGAGAG TGAATTTCTT 
GGAGAGATCC TCATAGAGTT GGAAACAGCG CTTTTAGATG ATGAGCCCCA TTGGTATAAA 
CTCCAGACAC ATGACGAATC TTCACTACCT CTGCCTCAGC CATCACCGTT CATGCCCAGG 
CGGCATATTC ATGGAGAGAG CTCCAGCAAA AAGCTACAAA GATCTCAGCG AATCAGTGAT 
AGTGACATCT CAGATTATGA GGTTGATGAT GGTATTGGAG TAGTGCCTCC AGTGGGTTAT 
AGAGCTAGTG CTAGAGAGAG TAAAGCAACC ACGTTAACAG TGCCAGAGCA ACAAAGAACT 
ACACATCACC GCTCACGTTC CGTGTCTCCT CATCGCGGCG ATGATCAGGG AAGGCCTCGT 
TCACGTTTAC CAAATGTGCC ATTACAGAGG AGCTTAGATG AAATTCATCC AACACGAAGG 
TCACGTTCTC CAACCCGACA CCATGATGCC TCCCGAAGCC CGGCCGATCA CAGATCCAGA 
CATGTGGAAA GTCAATATTC GTCAGAGCCA GACAGTGAGC TTCTCATGCT GCCCAGAGCA 
AAACGAGGAC GAAGTGCAGA AAGCCTACAC ATGACCAGTG AACTGCAGCC CTCTCTTGAC 
AGGGCTAGGA GTGCTAGTAC CAACTGCTTG AGACCAGATA CTAGTTTGCA TTCACCAGAA 
CGAGAAAGAA CGCACCGACA AGGAAGCCCA ACCCAGTCTC CTCCAGCAGA CACATCCTTC 
GGCAGTCGCC GTGGAAGACA GCTCCCACAG GTGCCAGTTC GAAGCGGCAG TATAGAACAA 
GCAAGCTTAG TAGTGGAGGA GCGAACGAGA CAGATGAAAA TGAAAGTTCA CCGATTTAAG 
CAGACAACAG GGTCTGGGTC TAGTCAAGAA CTTGACCACG AGCAATACTC CAAGTACAAC 
ATACATAAAG ATCAGTACAG AAGCTGTGAT AACGCGTCTG CCAAGTCTTC AGATAGTGAT 
GTCAGTGATG TGTCCGCCAT TTCCAGAGCC AGCAGTACCT CACGCCTCAG CAGCACAAGC 
TTTATGTCAG AGCAGTCTGA GCGCCCCAGG GGTAGGATCA GTTCATTTAC CCCCAAAATG 
CAAGGCAGAC GGATGGGGAC TTCAGGAAGA GCCATCATCA AGAGCACCAG TGTAAGTGGA 
GAGATATATA CACTGGAACG TAATGACGGT AGCCAGTCGG ACACGGCCGT GGGTACCGTC 
GGAGCCGGTG GAAAGAAACG AAGATCCAGC CTGAGCGCCA AAGTGGTAGC CATTGTGTCT 
CGAAGAAGCA GGAGCACGTC ACAGCTCAGC CAGACAGAGT CGGGCCACAA GAAGTTGAAA 
AGCACCATCC AGAGGAGTAC GGAAACAGGA ATGGCAGCTG AAATGCGGAA GATGGTGAGA 
CAGCCGAGCC GGGAGTCCAC GGATGGCAGC ATCAACAGTT ATAGCTCGGA AGGAAACTTG 
ATATTTCCTG GAGTTCGAGT AGGACCCGAC AGTCAGTTCA GTGATTTCCT TGATGGGTTG 
GGACCAGCGC AGCTCGTTGG CCGTCAGACG CTCGCCACCC CGGCCATGGG CGATATCCAA 
ATCGGGATGG AGGATAAGAA GGGTCAGTTG GAGGTTGAGG TTATCAGAGC CCGGAGCCTT 
ACACAAAAAC CTGGTTCCAA ATCTACACCC GCTCCCTATG TGAAAGTATA TCTTTTGGAA 
AATGGAGCCT GTATTGCCAA AAAGAAGACA AGAATTGCAC GGAAAACTCT CGATCCTTTG 
TATCAGCAGT CCCTGGTTTT TGATGAAAGT CCACAGGGTA AAGTTCTTCA GGTGATTGTC 
TGGGGTGACT ATGGAAGAAT GGACCACAAA TGCTTTATGG GTGTGGCTCA AATCTTGTTG 
GAAGAACTTG ATCTATCCAG CATGGTGATT GGATGGTATA AATTGTTCCC TCCGTCCTCA 
CTGGTGGATC CCACTCTCGC TCCCCTGACC CGCCGGGCTT CCCAATCATC TCTGGAAAGT 
TCGTCCGGGC CTCCCTGCAT CCGGTCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rims1 ( XM_017596668.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017452157.1 Rattus norvegicus regulating synaptic membrane exocytosis 1 (Rims1), transcript variant X15, mRNA.

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CloneID	ORa62885
Clone ID Related Accession (Same CDS sequence)	XM_017596668.1
Accession Version	XM_017596668.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4521bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	regulating synaptic membrane exocytosis protein 1 isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005108.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCTCGG CCGTGGGGCC CCGAGGTCCT CGCCCACCCA CGGTGCCTCC CCCTATGCAA 
GAACTGCCCG ACCTGAGCCA CCTGACCGAG GAGGAGAGGA ACATTATCAT GGCAGTGATG 
GACCGGCAGA AGGAAGAGGA GGAAAAAGAA GAGGCCATGC TCAAGTGTGT TGTCAGGGAC 
ATGGCGAAGC CTGCTGCCTG CAAAACACCA AGAAATGCTG AAAGCCAGCC CCATCAACCA 
CCACTGAGAT TGCATCAACA GTTTGAAAGC TACAAGGAGC AAGTGAGAAA AATCGGAGAG 
GAAGCGAGGC GTTACCAGGG CGAGCACAAG GATGATGCCC CGACGTGTGG AATCTGTCAT 
AAGACAAAGT TTGCTGATGG ATGTGGCCAT CTCTGCTCCT ATTGTCGCAC CAAGTTCTGT 
GCACGCTGCG GAGGCCGTGT GTCTCTGCGA TCGAACAATG AGGACAAAGT GGTTATGTGG 
GTATGCAATT TATGTCGAAA GCAACAAGAA ATCTTAACGA AATCTGGAGC GTGGTTCTTT 
GGAAGTGGCC CTCAGCAGCC TAGTCAAGAT GGGACTCTGA GTGACACGGC CACAGGTGCT 
GGATCTGAGG TGCCAAGAGA AAAGAAAGCA AGGCTCCAAG AGCGATCAAG GTCTCAGACG 
CCCTTGAGTA CAGCAGCTGT CTCTTCCCAA GACACTGCTA CCCCCGGTGC ACCGTTGGAC 
AGGAACAAAG GGGCTGAGCC CTCACAGCAA GCCTTGGGTC CTGAACAGAA GCAGGCATCA 
AGATCAAGAA GCGAGCCACC GAGGGAAAGG AAGAAGGCTC CAGGGCTTTC AGAGCAGAAT 
GGCAAGGGAG GCCAGAAGAG CGAGCGCAAA CGTGTCCCCA AGTCTGTGGT GCAACCCGGG 
GAAGGGATCG CGGATGAGAG GGAGAGGAAA GAGAGGCGGG AAACCCGCAG GTTGGAGAAA 
GGGCGCTCCC AGGACTACTC AGACCGGCCT GAGAAACGCG ACAATGGCAG GGTGGCGGAA 
GACCAGAAGC AGAGGAAGGA GGAGGAGTAC CAGACTAGGT ACCGCAGCGA CCCTAACCTG 
GCTCGCTACC CGGTGAAGGC GCCGCCAGAG GAGCAGCAGA TGCGCATGCA CGCCCGGGTG 
TCCCGAGCGA GGCACGAGCG GCGCCACAGC GACGTGGCGC TCCCGCACAC CGAGGCAGCT 
GCCGCCGCGC CGGCTGAGGC CACGGCGGGC AAGCGCGCGC CGGCCACCGC CAGGGTCTCT 
CCCCCGGAGT CCCCGCGCGC ACGCGCGCCC GCCGCCCAGC CTCCCACCGA GCACGGGCCA 
CCGCCGCCGC GGCCAGTCCC GGGTCCCGCA GAGCCACCCG AGCCGCGCGT CCCCGAGCCG 
CTCCGTAAGC AGGGCCGCCT GGACCCGGCC TCGGCCGTGC TTCTGCGCAA GGCCAAGCGC 
GAGAAGGCGG AGAGCATGCT GCGGAACGAC TCGCTGAGCT CCGATCAGTC CGAGTCCGTG 
CGGCCATCCC CGCCCAAGCC TCACCGGCCC AAGCGGGGAG GCAAGAGACG TCAGATGTCG 
GTGAGCAGCT CGGAGGAGGA GGGCGTGTCC ACACCGGAGT ACACGAGCTG CGAGGACGTG 
GAGCTGGAGA GCGAGAGCGT GAGCGAGAAA GGTGACTTGG ATTACTACTG GTTGGATCCC 
GCCACGTGGC ACAGCAGGGA AACGTCGCCT ATCAGTTCGC ATCCTGTAAC GTGGCAGCCG 
TCTAAAGAGG GAGATCGACT AATCGGCCGT GTTATTCTTA ACAAAAGAAC AACCATGCCC 
AAAGAATCAG GTGCATTATT GGGTCTGAAG GTGGTTGGAG GAAAAATGAC GGACTTAGGG 
CGCCTTGGTG CTTTCATCAC CAAAGTAAAG AAGGGCAGCC TGGCAGACGT CGTCGGACAC 
CTAAGAGCAG GGGACGAAGT CCTAGAGTGG AATGGTAAAC CCCTGCCGGG AGCAACAAAC 
GAAGAAGTTT ACAACATTAT CTTAGAATCA AAATCAGAAC CTCAAGTTGA GATTATTGTT 
TCAAGGCCTA TTGGTGACAT CCCCAGGATC CCTGAGAGTT CCCATCCTCC CCTGGAGTCC 
AGTTCAAGTT CCTTTGAATC TCAGAAAATG GAAAGGCCTT CTATTTCTGT TATTTCTCCA 
ACCAGCCCTG GAGCTCTGAA AGATGCCCCA CAAGTCTTAC CAGGGCAACT CTCAGTGAAG 
CTATGGTATG ATAAAGTGGG GCACCAGCTG ATTGTAAATG TTCTACAAGC AACAGATCTA 
CCCCCTAGAG TAGATGGCCG TCCCAGGAAT CCCTATGTAA AAATGTATTT TCTTCCAGAT 
AGAAGCGACA AAAGTAAAAG GAGAACCAAA ACAGTAAAGA AACTTCTAGA GCCAAAATGG 
AACCAGACAT TTGTCTACTC ACACGTACAT CGTAGAGATT TTCGAGAGCG AATGTTAGAG 
ATTACCGTGT GGGACCAGCC GAGAGTACAG GACGAAGAGA GTGAATTTCT TGGAGAGATC 
CTCATAGAGT TGGAAACAGC GCTTTTAGAT GATGAGCCCC ATTGGTATAA ACTCCAGACA 
CATGACGAAT CTTCACTACC TCTGCCTCAG CCATCACCGT TCATGCCCAG GCGGCATATT 
CATGGAGAGA GCTCCAGCAA AAAGCTACAA AGATCTCAGC GAATCAGTGA TAGTGACATC 
TCAGATTATG AGGTTGATGA TGGTATTGGA GTAGTGCCTC CAGTGGGTTA TAGAGCTAGT 
GCTAGAGAGA GTAAAGCAAC CACGTTAACA GTGCCAGAGC AACAAAGAAC TACACATCAC 
CGCTCACGTT CCGTGTCTCC TCATCGCGGC GATGATCAGG GAAGGCCTCG TTCACGTTTA 
CCAAATGTGC CATTACAGAG GAGCTTAGAT GAAATTCATC CAACACGAAG GTCACGTTCT 
CCAACCCGAC ACCATGATGC CTCCCGAAGC CCGGCCGATC ACAGATCCAG ACATGTGGAA 
AGTCAATATT CGTCAGAGCC AGACAGTGAG CTTCTCATGC TGCCCAGAGC AAAACGAGGA 
CGAAGTGCAG AAAGCCTACA CATGACCAGT GAACTGCAGC CCTCTCTTGA CAGGGCTAGG 
AGTGCTAGTA CCAACTGCTT GAGACCAGAT ACTAGTTTGC ATTCACCAGA ACGAGAAAGA 
ACGCACCGAC AAGGAAGCCC AACCCAGTCT CCTCCAGCAG ACACATCCTT CGGCAGTCGC 
CGTGGAAGAC AGCTCCCACA GGTGCCAGTT CGAAGCGGCA GTATAGAACA AGCAAGCTTA 
GTAGTGGAGG AGCGAACGAG ACAGATGAAA ATGAAAGTTC ACCGATTTAA GCAGACAACA 
GGGTCTGGGT CTAGTCAAGA ACTTGACCAC GAGCAATACT CCAAGTACAA CATACATAAA 
GATCAGTACA GAAGCTGTGA TAACGCGTCT GCCAAGTCTT CAGATAGTGA TGTCAGTGAT 
GTGTCCGCCA TTTCCAGAGC CAGCAGTACC TCACGCCTCA GCAGCACAAG CTTTATGTCA 
GAGCAGTCTG AGCGCCCCAG GGGTAGGATC AGTTCATTTA CCCCCAAAAT GCAAGGCAGA 
CGGATGGGGA CTTCAGGAAG AGCCATCATC AAGAGCACCA GTGTAAGTGG AGAGATATAT 
ACACTGGAAC GTAATGACGG TAGCCAGTCG GACACGGCCG TGGGTACCGT CGGAGCCGGT 
GGAAAGAAAC GAAGATCCAG CCTGAGCGCC AAAGTGGTAG CCATTGTGTC TCGAAGAAGC 
AGGAGCACGT CACAGCTCAG CCAGACAGAG TCGGGCCACA AGAAGTTGAA AAGCACCATC 
CAGAGGAGTA CGGAAACAGG AATGGCAGCT GAAATGCGGA AGATGGTGAG ACAGCCGAGC 
CGGGAGTCCA CGGATGGCAG CATCAACAGT TATAGCTCGG AAGGAAACTT GATATTTCCT 
GGAGTTCGAG TAGGACCCGA CAGTCAGTTC AGTGATTTCC TTGATGGGTT GGGACCAGCG 
CAGCTCGTTG GCCGTCAGAC GCTCGCCACC CCGGCCATGG GCGATATCCA AATCGGGATG 
GAGGATAAGA AGGGTCAGTT GGAGGTTGAG GTTATCAGAG CCCGGAGCCT TACACAAAAA 
CCTGGTTCCA AATCTACACC CGCTCCCTAT GTGAAAGTAT ATCTTTTGGA AAATGGAGCC 
TGTATTGCCA AAAAGAAGAC AAGAATTGCA CGGAAAACTC TCGATCCTTT GTATCAGCAG 
TCCCTGGTTT TTGATGAAAG TCCACAGGGT AAAGTTCTTC AGGTGATTGT CTGGGGTGAC 
TATGGAAGAA TGGACCACAA ATGCTTTATG GGTGTGGCTC AAATCTTGTT GGAAGAACTT 
GATCTATCCA GCATGGTGAT TGGATGGTAT AAATTGTTCC CTCCGTCCTC ACTGGTGGAT 
CCCACTCTCG CTCCCCTGAC CCGCCGGGCT TCCCAATCAT CTCTGGAAAG TTCGTCCGGG 
CCTCCCTGCA TCCGGTCATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017452157.1
CDS	306..4826
Translation	MSSAVGPRGPRPPTVPPPMQELPDLSHLTEEERNIIMAVMDRQKEEEEKEEAMLKCVVRDMAKPAACKTPRNAESQPHQPPLRLHQQFESYKEQVRKIGEEARRYQGEHKDDAPTCGICHKTKFADGCGHLCSYCRTKFCARCGGRVSLRSNNEDKVVMWVCNLCRKQQEILTKSGAWFFGSGPQQPSQDGTLSDTATGAGSEVPREKKARLQERSRSQTPLSTAAVSSQDTATPGAPLDRNKGAEPSQQALGPEQKQASRSRSEPPRERKKAPGLSEQNGKGGQKSERKRVPKSVVQPGEGIADERERKERRETRRLEKGRSQDYSDRPEKRDNGRVAEDQKQRKEEEYQTRYRSDPNLARYPVKAPPEEQQMRMHARVSRARHERRHSDVALPHTEAAAAAPAEATAGKRAPATARVSPPESPRARAPAAQPPTEHGPPPPRPVPGPAEPPEPRVPEPLRKQGRLDPASAVLLRKAKREKAESMLRNDSLSSDQSESVRPSPPKPHRPKRGGKRRQMSVSSSEEEGVSTPEYTSCEDVELESESVSEKGDLDYYWLDPATWHSRETSPISSHPVTWQPSKEGDRLIGRVILNKRTTMPKESGALLGLKVVGGKMTDLGRLGAFITKVKKGSLADVVGHLRAGDEVLEWNGKPLPGATNEEVYNIILESKSEPQVEIIVSRPIGDIPRIPESSHPPLESSSSSFESQKMERPSISVISPTSPGALKDAPQVLPGQLSVKLWYDKVGHQLIVNVLQATDLPPRVDGRPRNPYVKMYFLPDRSDKSKRRTKTVKKLLEPKWNQTFVYSHVHRRDFRERMLEITVWDQPRVQDEESEFLGEILIELETALLDDEPHWYKLQTHDESSLPLPQPSPFMPRRHIHGESSSKKLQRSQRISDSDISDYEVDDGIGVVPPVGYRASARESKATTLTVPEQQRTTHHRSRSVSPHRGDDQGRPRSRLPNVPLQRSLDEIHPTRRSRSPTRHHDASRSPADHRSRHVESQYSSEPDSELLMLPRAKRGRSAESLHMTSELQPSLDRARSASTNCLRPDTSLHSPERERTHRQGSPTQSPPADTSFGSRRGRQLPQVPVRSGSIEQASLVVEERTRQMKMKVHRFKQTTGSGSSQELDHEQYSKYNIHKDQYRSCDNASAKSSDSDVSDVSAISRASSTSRLSSTSFMSEQSERPRGRISSFTPKMQGRRMGTSGRAIIKSTSVSGEIYTLERNDGSQSDTAVGTVGAGGKKRRSSLSAKVVAIVSRRSRSTSQLSQTESGHKKLKSTIQRSTETGMAAEMRKMVRQPSRESTDGSINSYSSEGNLIFPGVRVGPDSQFSDFLDGLGPAQLVGRQTLATPAMGDIQIGMEDKKGQLEVEVIRARSLTQKPGSKSTPAPYVKVYLLENGACIAKKKTRIARKTLDPLYQQSLVFDESPQGKVLQVIVWGDYGRMDHKCFMGVAQILLEELDLSSMVIGWYKLFPPSSLVDPTLAPLTRRASQSSLESSSGPPCIRS

Target ORF information:

RefSeq Version	XM_017596668.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus regulating synaptic membrane exocytosis 1 (Rims1), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017596668.1

ORF Insert Sequence:

ATGTCCTCGG CCGTGGGGCC CCGAGGTCCT CGCCCACCCA CGGTGCCTCC CCCTATGCAA 
GAACTGCCCG ACCTGAGCCA CCTGACCGAG GAGGAGAGGA ACATTATCAT GGCAGTGATG 
GACCGGCAGA AGGAAGAGGA GGAAAAAGAA GAGGCCATGC TCAAGTGTGT TGTCAGGGAC 
ATGGCGAAGC CTGCTGCCTG CAAAACACCA AGAAATGCTG AAAGCCAGCC CCATCAACCA 
CCACTGAGAT TGCATCAACA GTTTGAAAGC TACAAGGAGC AAGTGAGAAA AATCGGAGAG 
GAAGCGAGGC GTTACCAGGG CGAGCACAAG GATGATGCCC CGACGTGTGG AATCTGTCAT 
AAGACAAAGT TTGCTGATGG ATGTGGCCAT CTCTGCTCCT ATTGTCGCAC CAAGTTCTGT 
GCACGCTGCG GAGGCCGTGT GTCTCTGCGA TCGAACAATG AGGACAAAGT GGTTATGTGG 
GTATGCAATT TATGTCGAAA GCAACAAGAA ATCTTAACGA AATCTGGAGC GTGGTTCTTT 
GGAAGTGGCC CTCAGCAGCC TAGTCAAGAT GGGACTCTGA GTGACACGGC CACAGGTGCT 
GGATCTGAGG TGCCAAGAGA AAAGAAAGCA AGGCTCCAAG AGCGATCAAG GTCTCAGACG 
CCCTTGAGTA CAGCAGCTGT CTCTTCCCAA GACACTGCTA CCCCCGGTGC ACCGTTGGAC 
AGGAACAAAG GGGCTGAGCC CTCACAGCAA GCCTTGGGTC CTGAACAGAA GCAGGCATCA 
AGATCAAGAA GCGAGCCACC GAGGGAAAGG AAGAAGGCTC CAGGGCTTTC AGAGCAGAAT 
GGCAAGGGAG GCCAGAAGAG CGAGCGCAAA CGTGTCCCCA AGTCTGTGGT GCAACCCGGG 
GAAGGGATCG CGGATGAGAG GGAGAGGAAA GAGAGGCGGG AAACCCGCAG GTTGGAGAAA 
GGGCGCTCCC AGGACTACTC AGACCGGCCT GAGAAACGCG ACAATGGCAG GGTGGCGGAA 
GACCAGAAGC AGAGGAAGGA GGAGGAGTAC CAGACTAGGT ACCGCAGCGA CCCTAACCTG 
GCTCGCTACC CGGTGAAGGC GCCGCCAGAG GAGCAGCAGA TGCGCATGCA CGCCCGGGTG 
TCCCGAGCGA GGCACGAGCG GCGCCACAGC GACGTGGCGC TCCCGCACAC CGAGGCAGCT 
GCCGCCGCGC CGGCTGAGGC CACGGCGGGC AAGCGCGCGC CGGCCACCGC CAGGGTCTCT 
CCCCCGGAGT CCCCGCGCGC ACGCGCGCCC GCCGCCCAGC CTCCCACCGA GCACGGGCCA 
CCGCCGCCGC GGCCAGTCCC GGGTCCCGCA GAGCCACCCG AGCCGCGCGT CCCCGAGCCG 
CTCCGTAAGC AGGGCCGCCT GGACCCGGCC TCGGCCGTGC TTCTGCGCAA GGCCAAGCGC 
GAGAAGGCGG AGAGCATGCT GCGGAACGAC TCGCTGAGCT CCGATCAGTC CGAGTCCGTG 
CGGCCATCCC CGCCCAAGCC TCACCGGCCC AAGCGGGGAG GCAAGAGACG TCAGATGTCG 
GTGAGCAGCT CGGAGGAGGA GGGCGTGTCC ACACCGGAGT ACACGAGCTG CGAGGACGTG 
GAGCTGGAGA GCGAGAGCGT GAGCGAGAAA GGTGACTTGG ATTACTACTG GTTGGATCCC 
GCCACGTGGC ACAGCAGGGA AACGTCGCCT ATCAGTTCGC ATCCTGTAAC GTGGCAGCCG 
TCTAAAGAGG GAGATCGACT AATCGGCCGT GTTATTCTTA ACAAAAGAAC AACCATGCCC 
AAAGAATCAG GTGCATTATT GGGTCTGAAG GTGGTTGGAG GAAAAATGAC GGACTTAGGG 
CGCCTTGGTG CTTTCATCAC CAAAGTAAAG AAGGGCAGCC TGGCAGACGT CGTCGGACAC 
CTAAGAGCAG GGGACGAAGT CCTAGAGTGG AATGGTAAAC CCCTGCCGGG AGCAACAAAC 
GAAGAAGTTT ACAACATTAT CTTAGAATCA AAATCAGAAC CTCAAGTTGA GATTATTGTT 
TCAAGGCCTA TTGGTGACAT CCCCAGGATC CCTGAGAGTT CCCATCCTCC CCTGGAGTCC 
AGTTCAAGTT CCTTTGAATC TCAGAAAATG GAAAGGCCTT CTATTTCTGT TATTTCTCCA 
ACCAGCCCTG GAGCTCTGAA AGATGCCCCA CAAGTCTTAC CAGGGCAACT CTCAGTGAAG 
CTATGGTATG ATAAAGTGGG GCACCAGCTG ATTGTAAATG TTCTACAAGC AACAGATCTA 
CCCCCTAGAG TAGATGGCCG TCCCAGGAAT CCCTATGTAA AAATGTATTT TCTTCCAGAT 
AGAAGCGACA AAAGTAAAAG GAGAACCAAA ACAGTAAAGA AACTTCTAGA GCCAAAATGG 
AACCAGACAT TTGTCTACTC ACACGTACAT CGTAGAGATT TTCGAGAGCG AATGTTAGAG 
ATTACCGTGT GGGACCAGCC GAGAGTACAG GACGAAGAGA GTGAATTTCT TGGAGAGATC 
CTCATAGAGT TGGAAACAGC GCTTTTAGAT GATGAGCCCC ATTGGTATAA ACTCCAGACA 
CATGACGAAT CTTCACTACC TCTGCCTCAG CCATCACCGT TCATGCCCAG GCGGCATATT 
CATGGAGAGA GCTCCAGCAA AAAGCTACAA AGATCTCAGC GAATCAGTGA TAGTGACATC 
TCAGATTATG AGGTTGATGA TGGTATTGGA GTAGTGCCTC CAGTGGGTTA TAGAGCTAGT 
GCTAGAGAGA GTAAAGCAAC CACGTTAACA GTGCCAGAGC AACAAAGAAC TACACATCAC 
CGCTCACGTT CCGTGTCTCC TCATCGCGGC GATGATCAGG GAAGGCCTCG TTCACGTTTA 
CCAAATGTGC CATTACAGAG GAGCTTAGAT GAAATTCATC CAACACGAAG GTCACGTTCT 
CCAACCCGAC ACCATGATGC CTCCCGAAGC CCGGCCGATC ACAGATCCAG ACATGTGGAA 
AGTCAATATT CGTCAGAGCC AGACAGTGAG CTTCTCATGC TGCCCAGAGC AAAACGAGGA 
CGAAGTGCAG AAAGCCTACA CATGACCAGT GAACTGCAGC CCTCTCTTGA CAGGGCTAGG 
AGTGCTAGTA CCAACTGCTT GAGACCAGAT ACTAGTTTGC ATTCACCAGA ACGAGAAAGA 
ACGCACCGAC AAGGAAGCCC AACCCAGTCT CCTCCAGCAG ACACATCCTT CGGCAGTCGC 
CGTGGAAGAC AGCTCCCACA GGTGCCAGTT CGAAGCGGCA GTATAGAACA AGCAAGCTTA 
GTAGTGGAGG AGCGAACGAG ACAGATGAAA ATGAAAGTTC ACCGATTTAA GCAGACAACA 
GGGTCTGGGT CTAGTCAAGA ACTTGACCAC GAGCAATACT CCAAGTACAA CATACATAAA 
GATCAGTACA GAAGCTGTGA TAACGCGTCT GCCAAGTCTT CAGATAGTGA TGTCAGTGAT 
GTGTCCGCCA TTTCCAGAGC CAGCAGTACC TCACGCCTCA GCAGCACAAG CTTTATGTCA 
GAGCAGTCTG AGCGCCCCAG GGGTAGGATC AGTTCATTTA CCCCCAAAAT GCAAGGCAGA 
CGGATGGGGA CTTCAGGAAG AGCCATCATC AAGAGCACCA GTGTAAGTGG AGAGATATAT 
ACACTGGAAC GTAATGACGG TAGCCAGTCG GACACGGCCG TGGGTACCGT CGGAGCCGGT 
GGAAAGAAAC GAAGATCCAG CCTGAGCGCC AAAGTGGTAG CCATTGTGTC TCGAAGAAGC 
AGGAGCACGT CACAGCTCAG CCAGACAGAG TCGGGCCACA AGAAGTTGAA AAGCACCATC 
CAGAGGAGTA CGGAAACAGG AATGGCAGCT GAAATGCGGA AGATGGTGAG ACAGCCGAGC 
CGGGAGTCCA CGGATGGCAG CATCAACAGT TATAGCTCGG AAGGAAACTT GATATTTCCT 
GGAGTTCGAG TAGGACCCGA CAGTCAGTTC AGTGATTTCC TTGATGGGTT GGGACCAGCG 
CAGCTCGTTG GCCGTCAGAC GCTCGCCACC CCGGCCATGG GCGATATCCA AATCGGGATG 
GAGGATAAGA AGGGTCAGTT GGAGGTTGAG GTTATCAGAG CCCGGAGCCT TACACAAAAA 
CCTGGTTCCA AATCTACACC CGCTCCCTAT GTGAAAGTAT ATCTTTTGGA AAATGGAGCC 
TGTATTGCCA AAAAGAAGAC AAGAATTGCA CGGAAAACTC TCGATCCTTT GTATCAGCAG 
TCCCTGGTTT TTGATGAAAG TCCACAGGGT AAAGTTCTTC AGGTGATTGT CTGGGGTGAC 
TATGGAAGAA TGGACCACAA ATGCTTTATG GGTGTGGCTC AAATCTTGTT GGAAGAACTT 
GATCTATCCA GCATGGTGAT TGGATGGTAT AAATTGTTCC CTCCGTCCTC ACTGGTGGAT 
CCCACTCTCG CTCCCCTGAC CCGCCGGGCT TCCCAATCAT CTCTGGAAAG TTCGTCCGGG 
CCTCCCTGCA TCCGGTCATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rims1 ( XM_017596669.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017452158.1 Rattus norvegicus regulating synaptic membrane exocytosis 1 (Rims1), transcript variant X16, mRNA.

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CloneID	ORa62886
Clone ID Related Accession (Same CDS sequence)	XM_017596669.1
Accession Version	XM_017596669.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4458bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	regulating synaptic membrane exocytosis protein 1 isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005108.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCTCGG CCGTGGGGCC CCGAGGTCCT CGCCCACCCA CGGTGCCTCC CCCTATGCAA 
GAACTGCCCG ACCTGAGCCA CCTGACCGAG GAGGAGAGGA ACATTATCAT GGCAGTGATG 
GACCGGCAGA AGGAAGAGGA GGAAAAAGAA GAGGCCATGC TCAAGTGTGT TGTCAGGGAC 
ATGGCGAAGC CTGCTGCCTG CAAAACACCA AGAAATGCTG AAAGCCAGCC CCATCAACCA 
CCACTGAACA TTTTCAGATG TGTCTGTGTT CCCAGAAAGC CAAGCAGCGA AGAGGGAGGC 
CCAGAAAGAG ACTGGAGATT GCATCAACAG TTTGAAAGCT ACAAGGAGCA AGTGAGAAAA 
ATCGGAGAGG AAGCGAGGCG TTACCAGGGC GAGCACAAGG ATGATGCCCC GACGTGTGGA 
ATCTGTCATA AGACAAAGTT TGCTGATGGA TGTGGCCATC TCTGCTCCTA TTGTCGCACC 
AAGTTCTGTG CACGCTGCGG AGGCCGTGTG TCTCTGCGAT CGAACAATGA GGACAAAGTG 
GTTATGTGGG TATGCAATTT ATGTCGAAAG CAACAAGAAA TCTTAACGAA ATCTGGAGCG 
TGGTTCTTTG GAAGTGGCCC TCAGCAGCCT AGTCAAGATG GGACTCTGAG TGACACGGCC 
ACAGGTGCTG GATCTGAGGT GCCAAGAGAA AAGAAAGCAA GGCTCCAAGA GCGATCAAGG 
TCTCAGACGC CCTTGAGTAC AGCAGCTGTC TCTTCCCAAG ACACTGCTAC CCCCGGTGCA 
CCGTTGGACA GGAACAAAGG GGCTGAGCCC TCACAGCAAG CCTTGGGTCC TGAACAGAAG 
CAGGCATCAA GATCAAGAAG CGAGCCACCG AGGGAAAGGA AGAAGGCTCC AGGGCTTTCA 
GAGCAGAATG GCAAGGGAGG CCAGAAGAGC GAGCGCAAAC GTGTCCCCAA GTCTGTGGTG 
CAACCCGGGG AAGGGATCGC GGATGAGAGG GAGAGGAAAG AGAGGCGGGA AACCCGCAGG 
TTGGAGAAAG GGCGCTCCCA GGACTACTCA GACCGGCCTG AGAAACGCGA CAATGGCAGG 
GTGGCGGAAG ACCAGAAGCA GAGGAAGGAG GAGGAGTACC AGACTAGGTA CCGCAGCGAC 
CCTAACCTGG CTCGCTACCC GGTGAAGGCG CCGCCAGAGG AGCAGCAGAT GCGCATGCAC 
GCCCGGGTGT CCCGAGCGAG GCACGAGCGG CGCCACAGCG ACGTGGCGCT CCCGCACACC 
GAGGCAGCTG CCGCCGCGCC GGCTGAGGCC ACGGCGGGCA AGCGCGCGCC GGCCACCGCC 
AGGGTCTCTC CCCCGGAGTC CCCGCGCGCA CGCGCGCCCG CCGCCCAGCC TCCCACCGAG 
CACGGGCCAC CGCCGCCGCG GCCAGTCCCG GGTCCCGCAG AGCCACCCGA GCCGCGCGTC 
CCCGAGCCGC TCCGTAAGCA GGGCCGCCTG GACCCGGCCT CGGCCGTGCT TCTGCGCAAG 
GCCAAGCGCG AGAAGGCGGA GAGCATGCTG CGGAACGACT CGCTGAGCTC CGATCAGTCC 
GAGTCCGTGC GGCCATCCCC GCCCAAGCCT CACCGGCCCA AGCGGGGAGG CAAGAGACGT 
CAGATGTCGG TGAGCAGCTC GGAGGAGGAG GGCGTGTCCA CACCGGAGTA CACGAGCTGC 
GAGGACGTGG AGCTGGAGAG CGAGAGCGTG AGCGAGAAAG GTGACTTGGA TTACTACTGG 
TTGGATCCCG CCACGTGGCA CAGCAGGGAA ACGTCGCCTA TCAGTTCGCA TCCTGTAACG 
TGGCAGCCGT CTAAAGAGGG AGATCGACTA ATCGGCCGTG TTATTCTTAA CAAAAGAACA 
ACCATGCCCA AAGAATCAGG TGCATTATTG GGTCTGAAGG TGGTTGGAGG AAAAATGACG 
GACTTAGGGC GCCTTGGTGC TTTCATCACC AAAGTAAAGA AGGGCAGCCT GGCAGACGTC 
GTCGGACACC TAAGAGCAGG GGACGAAGTC CTAGAGTGGA ATGGTAAACC CCTGCCGGGA 
GCAACAAACG AAGAAGTTTA CAACATTATC TTAGAATCAA AATCAGAACC TCAAGTTGAG 
ATTATTGTTT CAAGGCCTAT TGGTGACATC CCCAGGATCC CTGAGAGTTC CCATCCTCCC 
CTGGAGTCCA GTTCAAGTTC CTTTGAATCT CAGAAAATGG AAAGGCCTTC TATTTCTGTT 
ATTTCTCCAA CCAGCCCTGG AGCTCTGAAA GATGCCCCAC AAGTCTTACC AGGGCAACTC 
TCAGTGAAGC TATGGTATGA TAAAGTGGGG CACCAGCTGA TTGTAAATGT TCTACAAGCA 
ACAGATCTAC CCCCTAGAGT AGATGGCCGT CCCAGGAATC CCTATGTAAA AATGTATTTT 
CTTCCAGATA GAAGCGACAA AAGTAAAAGG AGAACCAAAA CAGTAAAGAA ACTTCTAGAG 
CCAAAATGGA ACCAGACATT TGTCTACTCA CACGTACATC GTAGAGATTT TCGAGAGCGA 
ATGTTAGAGA TTACCGTGTG GGACCAGCCG AGAGTACAGG ACGAAGAGAG TGAATTTCTT 
GGAGAGATCC TCATAGAGTT GGAAACAGCG CTTTTAGATG ATGAGCCCCA TTGGTATAAA 
CTCCAGACAC ATGACGAATC TTCACTACCT CTGCCTCAGC CATCACCGTT CATGCCCAGG 
CGGCATATTC ATGGAGAGAG CTCCAGCAAA AAGCTACAAA GATCTCAGCG AATCAGTGAT 
AGTGACATCT CAGATTATGA GGTTGATGAT GGTATTGGAG TAGTGCCTCC AGTGGGTTAT 
AGAGCTAGTG CTAGAGAGAG TAAAGCAACC ACGTTAACAG TGCCAGAGCA ACAAAGAACT 
ACACATCACC GCTCACGTTC CGTGTCTCCT CATCGCGGCG ATGATCAGGG AAGGCCTCGT 
TCACGTTTAC CAAATGTGCC ATTACAGAGG AGCTTAGATG AAATTCATCC AACACGAAGG 
TCACGTTCTC CAACCCGACA CCATGATGCC TCCCGAAGCC CGGCCGATCA CAGATCCAGA 
CATGTGGAAA GTCAATATTC GTCAGAGCCA GACAGAACGC ACCGACAAGG AAGCCCAACC 
CAGTCTCCTC CAGCAGACAC ATCCTTCGGC AGTCGCCGTG GAAGACAGCT CCCACAGGTG 
CCAGTTCGAA GCGGCAGTAT AGAACAAGCA AGCTTAGTAG TGGAGGAGCG AACGAGACAG 
ATGAAAATGA AAGTTCACCG ATTTAAGCAG ACAACAGGGT CTGGGTCTAG TCAAGAACTT 
GACCACGAGC AATACTCCAA GAAGTCACGT TACATTATGG AGTACAACAT ACATAAAGAT 
CAGTACAGAA GCTGTGATAA CGCGTCTGCC AAGTCTTCAG ATAGTGATGT CAGTGATGTG 
TCCGCCATTT CCAGAGCCAG CAGTACCTCA CGCCTCAGCA GCACAAGCTT TATGTCAGAG 
CAGTCTGAGC GCCCCAGGGG TAGGATCAGT TCATTTACCC CCAAAATGCA AGGCAGACGG 
ATGGGGACTT CAGGAAGAGC CATCATCAAG AGCACCAGTG TAAGTGGAGA GATATATACA 
CTGGAACGTA ATGACGGTAG CCAGTCGGAC ACGGCCGTGG GTACCGTCGG AGCCGGTGGA 
AAGAAACGAA GATCCAGCCT GAGCGCCAAA GTGGTAGCCA TTGTGTCTCG AAGAAGCAGG 
AGCACGTCAC AGCTCAGCCA GACAGAGTCG GGCCACAAGA AGTTGAAAAG CACCATCCAG 
AGGAGTACGG AAACAGGAAT GGCAGCTGAA ATGCGGAAGA TGGTGAGACA GCCGAGCCGG 
GAGTCCACGG ATGGCAGCAT CAACAGTTAT AGCTCGGAAG GAAACTTGAT ATTTCCTGGA 
GTTCGAGTAG GACCCGACAG TCAGTTCAGT GATTTCCTTG ATGGGTTGGG ACCAGCGCAG 
CTCGTTGGCC GTCAGACGCT CGCCACCCCG GCCATGGGCG ATATCCAAAT CGGGATGGAG 
GATAAGAAGG GTCAGTTGGA GGTTGAGGTT ATCAGAGCCC GGAGCCTTAC ACAAAAACCT 
GGTTCCAAAT CTACACCCGC TCCCTATGTG AAAGTATATC TTTTGGAAAA TGGAGCCTGT 
ATTGCCAAAA AGAAGACAAG AATTGCACGG AAAACTCTCG ATCCTTTGTA TCAGCAGTCC 
CTGGTTTTTG ATGAAAGTCC ACAGGGTAAA GTTCTTCAGG TGATTGTCTG GGGTGACTAT 
GGAAGAATGG ACCACAAATG CTTTATGGGT GTGGCTCAAA TCTTGTTGGA AGAACTTGAT 
CTATCCAGCA TGGTGATTGG ATGGTATAAA TTGTTCCCTC CGTCCTCACT GGTGGATCCC 
ACTCTCGCTC CCCTGACCCG CCGGGCTTCC CAATCATCTC TGGAAAGTTC GTCCGGGCCT 
CCCTGCATCC GGTCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017452158.1
CDS	307..4764
Translation	MSSAVGPRGPRPPTVPPPMQELPDLSHLTEEERNIIMAVMDRQKEEEEKEEAMLKCVVRDMAKPAACKTPRNAESQPHQPPLNIFRCVCVPRKPSSEEGGPERDWRLHQQFESYKEQVRKIGEEARRYQGEHKDDAPTCGICHKTKFADGCGHLCSYCRTKFCARCGGRVSLRSNNEDKVVMWVCNLCRKQQEILTKSGAWFFGSGPQQPSQDGTLSDTATGAGSEVPREKKARLQERSRSQTPLSTAAVSSQDTATPGAPLDRNKGAEPSQQALGPEQKQASRSRSEPPRERKKAPGLSEQNGKGGQKSERKRVPKSVVQPGEGIADERERKERRETRRLEKGRSQDYSDRPEKRDNGRVAEDQKQRKEEEYQTRYRSDPNLARYPVKAPPEEQQMRMHARVSRARHERRHSDVALPHTEAAAAAPAEATAGKRAPATARVSPPESPRARAPAAQPPTEHGPPPPRPVPGPAEPPEPRVPEPLRKQGRLDPASAVLLRKAKREKAESMLRNDSLSSDQSESVRPSPPKPHRPKRGGKRRQMSVSSSEEEGVSTPEYTSCEDVELESESVSEKGDLDYYWLDPATWHSRETSPISSHPVTWQPSKEGDRLIGRVILNKRTTMPKESGALLGLKVVGGKMTDLGRLGAFITKVKKGSLADVVGHLRAGDEVLEWNGKPLPGATNEEVYNIILESKSEPQVEIIVSRPIGDIPRIPESSHPPLESSSSSFESQKMERPSISVISPTSPGALKDAPQVLPGQLSVKLWYDKVGHQLIVNVLQATDLPPRVDGRPRNPYVKMYFLPDRSDKSKRRTKTVKKLLEPKWNQTFVYSHVHRRDFRERMLEITVWDQPRVQDEESEFLGEILIELETALLDDEPHWYKLQTHDESSLPLPQPSPFMPRRHIHGESSSKKLQRSQRISDSDISDYEVDDGIGVVPPVGYRASARESKATTLTVPEQQRTTHHRSRSVSPHRGDDQGRPRSRLPNVPLQRSLDEIHPTRRSRSPTRHHDASRSPADHRSRHVESQYSSEPDRTHRQGSPTQSPPADTSFGSRRGRQLPQVPVRSGSIEQASLVVEERTRQMKMKVHRFKQTTGSGSSQELDHEQYSKKSRYIMEYNIHKDQYRSCDNASAKSSDSDVSDVSAISRASSTSRLSSTSFMSEQSERPRGRISSFTPKMQGRRMGTSGRAIIKSTSVSGEIYTLERNDGSQSDTAVGTVGAGGKKRRSSLSAKVVAIVSRRSRSTSQLSQTESGHKKLKSTIQRSTETGMAAEMRKMVRQPSRESTDGSINSYSSEGNLIFPGVRVGPDSQFSDFLDGLGPAQLVGRQTLATPAMGDIQIGMEDKKGQLEVEVIRARSLTQKPGSKSTPAPYVKVYLLENGACIAKKKTRIARKTLDPLYQQSLVFDESPQGKVLQVIVWGDYGRMDHKCFMGVAQILLEELDLSSMVIGWYKLFPPSSLVDPTLAPLTRRASQSSLESSSGPPCIRS

Target ORF information:

RefSeq Version	XM_017596669.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus regulating synaptic membrane exocytosis 1 (Rims1), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017596669.1

ORF Insert Sequence:

ATGTCCTCGG CCGTGGGGCC CCGAGGTCCT CGCCCACCCA CGGTGCCTCC CCCTATGCAA 
GAACTGCCCG ACCTGAGCCA CCTGACCGAG GAGGAGAGGA ACATTATCAT GGCAGTGATG 
GACCGGCAGA AGGAAGAGGA GGAAAAAGAA GAGGCCATGC TCAAGTGTGT TGTCAGGGAC 
ATGGCGAAGC CTGCTGCCTG CAAAACACCA AGAAATGCTG AAAGCCAGCC CCATCAACCA 
CCACTGAACA TTTTCAGATG TGTCTGTGTT CCCAGAAAGC CAAGCAGCGA AGAGGGAGGC 
CCAGAAAGAG ACTGGAGATT GCATCAACAG TTTGAAAGCT ACAAGGAGCA AGTGAGAAAA 
ATCGGAGAGG AAGCGAGGCG TTACCAGGGC GAGCACAAGG ATGATGCCCC GACGTGTGGA 
ATCTGTCATA AGACAAAGTT TGCTGATGGA TGTGGCCATC TCTGCTCCTA TTGTCGCACC 
AAGTTCTGTG CACGCTGCGG AGGCCGTGTG TCTCTGCGAT CGAACAATGA GGACAAAGTG 
GTTATGTGGG TATGCAATTT ATGTCGAAAG CAACAAGAAA TCTTAACGAA ATCTGGAGCG 
TGGTTCTTTG GAAGTGGCCC TCAGCAGCCT AGTCAAGATG GGACTCTGAG TGACACGGCC 
ACAGGTGCTG GATCTGAGGT GCCAAGAGAA AAGAAAGCAA GGCTCCAAGA GCGATCAAGG 
TCTCAGACGC CCTTGAGTAC AGCAGCTGTC TCTTCCCAAG ACACTGCTAC CCCCGGTGCA 
CCGTTGGACA GGAACAAAGG GGCTGAGCCC TCACAGCAAG CCTTGGGTCC TGAACAGAAG 
CAGGCATCAA GATCAAGAAG CGAGCCACCG AGGGAAAGGA AGAAGGCTCC AGGGCTTTCA 
GAGCAGAATG GCAAGGGAGG CCAGAAGAGC GAGCGCAAAC GTGTCCCCAA GTCTGTGGTG 
CAACCCGGGG AAGGGATCGC GGATGAGAGG GAGAGGAAAG AGAGGCGGGA AACCCGCAGG 
TTGGAGAAAG GGCGCTCCCA GGACTACTCA GACCGGCCTG AGAAACGCGA CAATGGCAGG 
GTGGCGGAAG ACCAGAAGCA GAGGAAGGAG GAGGAGTACC AGACTAGGTA CCGCAGCGAC 
CCTAACCTGG CTCGCTACCC GGTGAAGGCG CCGCCAGAGG AGCAGCAGAT GCGCATGCAC 
GCCCGGGTGT CCCGAGCGAG GCACGAGCGG CGCCACAGCG ACGTGGCGCT CCCGCACACC 
GAGGCAGCTG CCGCCGCGCC GGCTGAGGCC ACGGCGGGCA AGCGCGCGCC GGCCACCGCC 
AGGGTCTCTC CCCCGGAGTC CCCGCGCGCA CGCGCGCCCG CCGCCCAGCC TCCCACCGAG 
CACGGGCCAC CGCCGCCGCG GCCAGTCCCG GGTCCCGCAG AGCCACCCGA GCCGCGCGTC 
CCCGAGCCGC TCCGTAAGCA GGGCCGCCTG GACCCGGCCT CGGCCGTGCT TCTGCGCAAG 
GCCAAGCGCG AGAAGGCGGA GAGCATGCTG CGGAACGACT CGCTGAGCTC CGATCAGTCC 
GAGTCCGTGC GGCCATCCCC GCCCAAGCCT CACCGGCCCA AGCGGGGAGG CAAGAGACGT 
CAGATGTCGG TGAGCAGCTC GGAGGAGGAG GGCGTGTCCA CACCGGAGTA CACGAGCTGC 
GAGGACGTGG AGCTGGAGAG CGAGAGCGTG AGCGAGAAAG GTGACTTGGA TTACTACTGG 
TTGGATCCCG CCACGTGGCA CAGCAGGGAA ACGTCGCCTA TCAGTTCGCA TCCTGTAACG 
TGGCAGCCGT CTAAAGAGGG AGATCGACTA ATCGGCCGTG TTATTCTTAA CAAAAGAACA 
ACCATGCCCA AAGAATCAGG TGCATTATTG GGTCTGAAGG TGGTTGGAGG AAAAATGACG 
GACTTAGGGC GCCTTGGTGC TTTCATCACC AAAGTAAAGA AGGGCAGCCT GGCAGACGTC 
GTCGGACACC TAAGAGCAGG GGACGAAGTC CTAGAGTGGA ATGGTAAACC CCTGCCGGGA 
GCAACAAACG AAGAAGTTTA CAACATTATC TTAGAATCAA AATCAGAACC TCAAGTTGAG 
ATTATTGTTT CAAGGCCTAT TGGTGACATC CCCAGGATCC CTGAGAGTTC CCATCCTCCC 
CTGGAGTCCA GTTCAAGTTC CTTTGAATCT CAGAAAATGG AAAGGCCTTC TATTTCTGTT 
ATTTCTCCAA CCAGCCCTGG AGCTCTGAAA GATGCCCCAC AAGTCTTACC AGGGCAACTC 
TCAGTGAAGC TATGGTATGA TAAAGTGGGG CACCAGCTGA TTGTAAATGT TCTACAAGCA 
ACAGATCTAC CCCCTAGAGT AGATGGCCGT CCCAGGAATC CCTATGTAAA AATGTATTTT 
CTTCCAGATA GAAGCGACAA AAGTAAAAGG AGAACCAAAA CAGTAAAGAA ACTTCTAGAG 
CCAAAATGGA ACCAGACATT TGTCTACTCA CACGTACATC GTAGAGATTT TCGAGAGCGA 
ATGTTAGAGA TTACCGTGTG GGACCAGCCG AGAGTACAGG ACGAAGAGAG TGAATTTCTT 
GGAGAGATCC TCATAGAGTT GGAAACAGCG CTTTTAGATG ATGAGCCCCA TTGGTATAAA 
CTCCAGACAC ATGACGAATC TTCACTACCT CTGCCTCAGC CATCACCGTT CATGCCCAGG 
CGGCATATTC ATGGAGAGAG CTCCAGCAAA AAGCTACAAA GATCTCAGCG AATCAGTGAT 
AGTGACATCT CAGATTATGA GGTTGATGAT GGTATTGGAG TAGTGCCTCC AGTGGGTTAT 
AGAGCTAGTG CTAGAGAGAG TAAAGCAACC ACGTTAACAG TGCCAGAGCA ACAAAGAACT 
ACACATCACC GCTCACGTTC CGTGTCTCCT CATCGCGGCG ATGATCAGGG AAGGCCTCGT 
TCACGTTTAC CAAATGTGCC ATTACAGAGG AGCTTAGATG AAATTCATCC AACACGAAGG 
TCACGTTCTC CAACCCGACA CCATGATGCC TCCCGAAGCC CGGCCGATCA CAGATCCAGA 
CATGTGGAAA GTCAATATTC GTCAGAGCCA GACAGAACGC ACCGACAAGG AAGCCCAACC 
CAGTCTCCTC CAGCAGACAC ATCCTTCGGC AGTCGCCGTG GAAGACAGCT CCCACAGGTG 
CCAGTTCGAA GCGGCAGTAT AGAACAAGCA AGCTTAGTAG TGGAGGAGCG AACGAGACAG 
ATGAAAATGA AAGTTCACCG ATTTAAGCAG ACAACAGGGT CTGGGTCTAG TCAAGAACTT 
GACCACGAGC AATACTCCAA GAAGTCACGT TACATTATGG AGTACAACAT ACATAAAGAT 
CAGTACAGAA GCTGTGATAA CGCGTCTGCC AAGTCTTCAG ATAGTGATGT CAGTGATGTG 
TCCGCCATTT CCAGAGCCAG CAGTACCTCA CGCCTCAGCA GCACAAGCTT TATGTCAGAG 
CAGTCTGAGC GCCCCAGGGG TAGGATCAGT TCATTTACCC CCAAAATGCA AGGCAGACGG 
ATGGGGACTT CAGGAAGAGC CATCATCAAG AGCACCAGTG TAAGTGGAGA GATATATACA 
CTGGAACGTA ATGACGGTAG CCAGTCGGAC ACGGCCGTGG GTACCGTCGG AGCCGGTGGA 
AAGAAACGAA GATCCAGCCT GAGCGCCAAA GTGGTAGCCA TTGTGTCTCG AAGAAGCAGG 
AGCACGTCAC AGCTCAGCCA GACAGAGTCG GGCCACAAGA AGTTGAAAAG CACCATCCAG 
AGGAGTACGG AAACAGGAAT GGCAGCTGAA ATGCGGAAGA TGGTGAGACA GCCGAGCCGG 
GAGTCCACGG ATGGCAGCAT CAACAGTTAT AGCTCGGAAG GAAACTTGAT ATTTCCTGGA 
GTTCGAGTAG GACCCGACAG TCAGTTCAGT GATTTCCTTG ATGGGTTGGG ACCAGCGCAG 
CTCGTTGGCC GTCAGACGCT CGCCACCCCG GCCATGGGCG ATATCCAAAT CGGGATGGAG 
GATAAGAAGG GTCAGTTGGA GGTTGAGGTT ATCAGAGCCC GGAGCCTTAC ACAAAAACCT 
GGTTCCAAAT CTACACCCGC TCCCTATGTG AAAGTATATC TTTTGGAAAA TGGAGCCTGT 
ATTGCCAAAA AGAAGACAAG AATTGCACGG AAAACTCTCG ATCCTTTGTA TCAGCAGTCC 
CTGGTTTTTG ATGAAAGTCC ACAGGGTAAA GTTCTTCAGG TGATTGTCTG GGGTGACTAT 
GGAAGAATGG ACCACAAATG CTTTATGGGT GTGGCTCAAA TCTTGTTGGA AGAACTTGAT 
CTATCCAGCA TGGTGATTGG ATGGTATAAA TTGTTCCCTC CGTCCTCACT GGTGGATCCC 
ACTCTCGCTC CCCTGACCCG CCGGGCTTCC CAATCATCTC TGGAAAGTTC GTCCGGGCCT 
CCCTGCATCC GGTCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rims1 ( XM_017596670.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017452159.1 Rattus norvegicus regulating synaptic membrane exocytosis 1 (Rims1), transcript variant X17, mRNA.

Price & Availability↑

CloneID	ORa62887
Clone ID Related Accession (Same CDS sequence)	XM_017596670.1
Accession Version	XM_017596670.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4437bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	regulating synaptic membrane exocytosis protein 1 isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005108.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCTCGG CCGTGGGGCC CCGAGGTCCT CGCCCACCCA CGGTGCCTCC CCCTATGCAA 
GAACTGCCCG ACCTGAGCCA CCTGACCGAG GAGGAGAGGA ACATTATCAT GGCAGTGATG 
GACCGGCAGA AGGAAGAGGA GGAAAAAGAA GAGGCCATGC TCAAGTGTGT TGTCAGGGAC 
ATGGCGAAGC CTGCTGCCTG CAAAACACCA AGAAATGCTG AAAGCCAGCC CCATCAACCA 
CCACTGAACA TTTTCAGATG TGTCTGTGTT CCCAGAAAGC CAAGCAGCGA AGAGGGAGGC 
CCAGAAAGAG ACTGGAGATT GCATCAACAG TTTGAAAGCT ACAAGGAGCA AGTGAGAAAA 
ATCGGAGAGG AAGCGAGGCG TTACCAGGGC GAGCACAAGG ATGATGCCCC GACGTGTGGA 
ATCTGTCATA AGACAAAGTT TGCTGATGGA TGTGGCCATC TCTGCTCCTA TTGTCGCACC 
AAGTTCTGTG CACGCTGCGG AGGCCGTGTG TCTCTGCGAT CGAACAATGA GGACAAAGTG 
GTTATGTGGG TATGCAATTT ATGTCGAAAG CAACAAGAAA TCTTAACGAA ATCTGGAGCG 
TGGTTCTTTG GAAGTGGCCC TCAGCAGCCT AGTCAAGATG GGACTCTGAG TGACACGGCC 
ACAGGTGCTG GATCTGAGGT GCCAAGAGAA AAGAAAGCAA GGCTCCAAGA GCGATCAAGG 
TCTCAGACGC CCTTGAGTAC AGCAGCTGTC TCTTCCCAAG ACACTGCTAC CCCCGGTGCA 
CCGTTGGACA GGAACAAAGG GGCTGAGCCC TCACAGCAAG CCTTGGGTCC TGAACAGAAG 
CAGGCATCAA GATCAAGAAG CGAGCCACCG AGGGAAAGGA AGAAGGCTCC AGGGCTTTCA 
GAGCAGAATG GCAAGGGAGG CCAGAAGAGC GAGCGCAAAC GTGTCCCCAA GTCTGTGGTG 
CAACCCGGGG AAGGGATCGC GGATGAGAGG GAGAGGAAAG AGAGGCGGGA AACCCGCAGG 
TTGGAGAAAG GGCGCTCCCA GGACTACTCA GACCGGCCTG AGAAACGCGA CAATGGCAGG 
GTGGCGGAAG ACCAGAAGCA GAGGAAGGAG GAGGAGTACC AGACTAGGTA CCGCAGCGAC 
CCTAACCTGG CTCGCTACCC GGTGAAGGCG CCGCCAGAGG AGCAGCAGAT GCGCATGCAC 
GCCCGGGTGT CCCGAGCGAG GCACGAGCGG CGCCACAGCG ACGTGGCGCT CCCGCACACC 
GAGGCAGCTG CCGCCGCGCC GGCTGAGGCC ACGGCGGGCA AGCGCGCGCC GGCCACCGCC 
AGGGTCTCTC CCCCGGAGTC CCCGCGCGCA CGCGCGCCCG CCGCCCAGCC TCCCACCGAG 
CACGGGCCAC CGCCGCCGCG GCCAGTCCCG GGTCCCGCAG AGCCACCCGA GCCGCGCGTC 
CCCGAGCCGC TCCGTAAGCA GGGCCGCCTG GACCCGGCCT CGGCCGTGCT TCTGCGCAAG 
GCCAAGCGCG AGAAGGCGGA GAGCATGCTG CGGAACGACT CGCTGAGCTC CGATCAGTCC 
GAGTCCGTGC GGCCATCCCC GCCCAAGCCT CACCGGCCCA AGCGGGGAGG CAAGAGACGT 
CAGATGTCGG TGAGCAGCTC GGAGGAGGAG GGCGTGTCCA CACCGGAGTA CACGAGCTGC 
GAGGACGTGG AGCTGGAGAG CGAGAGCGTG AGCGAGAAAG GTGACTTGGA TTACTACTGG 
TTGGATCCCG CCACGTGGCA CAGCAGGGAA ACGTCGCCTA TCAGTTCGCA TCCTGTAACG 
TGGCAGCCGT CTAAAGAGGG AGATCGACTA ATCGGCCGTG TTATTCTTAA CAAAAGAACA 
ACCATGCCCA AAGAATCAGG TGCATTATTG GGTCTGAAGG TGGTTGGAGG AAAAATGACG 
GACTTAGGGC GCCTTGGTGC TTTCATCACC AAAGTAAAGA AGGGCAGCCT GGCAGACGTC 
GTCGGACACC TAAGAGCAGG GGACGAAGTC CTAGAGTGGA ATGGTAAACC CCTGCCGGGA 
GCAACAAACG AAGAAGTTTA CAACATTATC TTAGAATCAA AATCAGAACC TCAAGTTGAG 
ATTATTGTTT CAAGGCCTAT TGGTGACATC CCCAGGATCC CTGAGAGTTC CCATCCTCCC 
CTGGAGTCCA GTTCAAGTTC CTTTGAATCT CAGAAAATGG AAAGGCCTTC TATTTCTGTT 
ATTTCTCCAA CCAGCCCTGG AGCTCTGAAA GATGCCCCAC AAGTCTTACC AGGGCAACTC 
TCAGTGAAGC TATGGTATGA TAAAGTGGGG CACCAGCTGA TTGTAAATGT TCTACAAGCA 
ACAGATCTAC CCCCTAGAGT AGATGGCCGT CCCAGGAATC CCTATGTAAA AATGTATTTT 
CTTCCAGATA GAAGCGACAA AAGTAAAAGG AGAACCAAAA CAGTAAAGAA ACTTCTAGAG 
CCAAAATGGA ACCAGACATT TGTCTACTCA CACGTACATC GTAGAGATTT TCGAGAGCGA 
ATGTTAGAGA TTACCGTGTG GGACCAGCCG AGAGTACAGG ACGAAGAGAG TGAATTTCTT 
GGAGAGATCC TCATAGAGTT GGAAACAGCG CTTTTAGATG ATGAGCCCCA TTGGTATAAA 
CTCCAGACAC ATGACGAATC TTCACTACCT CTGCCTCAGC CATCACCGTT CATGCCCAGG 
CGGCATATTC ATGGAGAGAG CTCCAGCAAA AAGCTACAAA GATCTCAGCG AATCAGTGAT 
AGTGACATCT CAGATTATGA GGTTGATGAT GGTATTGGAG TAGTGCCTCC AGTGGGTTAT 
AGAGCTAGTG CTAGAGAGAG TAAAGCAACC ACGTTAACAG TGCCAGAGCA ACAAAGAACT 
ACACATCACC GCTCACGTTC CGTGTCTCCT CATCGCGGCG ATGATCAGGG AAGGCCTCGT 
TCACGTTTAC CAAATGTGCC ATTACAGAGG AGCTTAGATG AAATTCATCC AACACGAAGG 
TCACGTTCTC CAACCCGACA CCATGATGCC TCCCGAAGCC CGGCCGATCA CAGATCCAGA 
CATGTGGAAA GTCAATATTC GTCAGAGCCA GACAGAACGC ACCGACAAGG AAGCCCAACC 
CAGTCTCCTC CAGCAGACAC ATCCTTCGGC AGTCGCCGTG GAAGACAGCT CCCACAGGTG 
CCAGTTCGAA GCGGCAGTAT AGAACAAGCA AGCTTAGTAG TGGAGGAGCG AACGAGACAG 
ATGAAAATGA AAGTTCACCG ATTTAAGCAG ACAACAGGGT CTGGGTCTAG TCAAGAACTT 
GACCACGAGC AATACTCCAA GTACAACATA CATAAAGATC AGTACAGAAG CTGTGATAAC 
GCGTCTGCCA AGTCTTCAGA TAGTGATGTC AGTGATGTGT CCGCCATTTC CAGAGCCAGC 
AGTACCTCAC GCCTCAGCAG CACAAGCTTT ATGTCAGAGC AGTCTGAGCG CCCCAGGGGT 
AGGATCAGTT CATTTACCCC CAAAATGCAA GGCAGACGGA TGGGGACTTC AGGAAGAGCC 
ATCATCAAGA GCACCAGTGT AAGTGGAGAG ATATATACAC TGGAACGTAA TGACGGTAGC 
CAGTCGGACA CGGCCGTGGG TACCGTCGGA GCCGGTGGAA AGAAACGAAG ATCCAGCCTG 
AGCGCCAAAG TGGTAGCCAT TGTGTCTCGA AGAAGCAGGA GCACGTCACA GCTCAGCCAG 
ACAGAGTCGG GCCACAAGAA GTTGAAAAGC ACCATCCAGA GGAGTACGGA AACAGGAATG 
GCAGCTGAAA TGCGGAAGAT GGTGAGACAG CCGAGCCGGG AGTCCACGGA TGGCAGCATC 
AACAGTTATA GCTCGGAAGG AAACTTGATA TTTCCTGGAG TTCGAGTAGG ACCCGACAGT 
CAGTTCAGTG ATTTCCTTGA TGGGTTGGGA CCAGCGCAGC TCGTTGGCCG TCAGACGCTC 
GCCACCCCGG CCATGGGCGA TATCCAAATC GGGATGGAGG ATAAGAAGGG TCAGTTGGAG 
GTTGAGGTTA TCAGAGCCCG GAGCCTTACA CAAAAACCTG GTTCCAAATC TACACCCGCT 
CCCTATGTGA AAGTATATCT TTTGGAAAAT GGAGCCTGTA TTGCCAAAAA GAAGACAAGA 
ATTGCACGGA AAACTCTCGA TCCTTTGTAT CAGCAGTCCC TGGTTTTTGA TGAAAGTCCA 
CAGGGTAAAG TTCTTCAGGT GATTGTCTGG GGTGACTATG GAAGAATGGA CCACAAATGC 
TTTATGGGTG TGGCTCAAAT CTTGTTGGAA GAACTTGATC TATCCAGCAT GGTGATTGGA 
TGGTATAAAT TGTTCCCTCC GTCCTCACTG GTGGATCCCA CTCTCGCTCC CCTGACCCGC 
CGGGCTTCCC AATCATCTCT GGAAAGTTCG TCCGGGCCTC CCTGCATCCG GTCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017452159.1
CDS	306..4742
Translation	MSSAVGPRGPRPPTVPPPMQELPDLSHLTEEERNIIMAVMDRQKEEEEKEEAMLKCVVRDMAKPAACKTPRNAESQPHQPPLNIFRCVCVPRKPSSEEGGPERDWRLHQQFESYKEQVRKIGEEARRYQGEHKDDAPTCGICHKTKFADGCGHLCSYCRTKFCARCGGRVSLRSNNEDKVVMWVCNLCRKQQEILTKSGAWFFGSGPQQPSQDGTLSDTATGAGSEVPREKKARLQERSRSQTPLSTAAVSSQDTATPGAPLDRNKGAEPSQQALGPEQKQASRSRSEPPRERKKAPGLSEQNGKGGQKSERKRVPKSVVQPGEGIADERERKERRETRRLEKGRSQDYSDRPEKRDNGRVAEDQKQRKEEEYQTRYRSDPNLARYPVKAPPEEQQMRMHARVSRARHERRHSDVALPHTEAAAAAPAEATAGKRAPATARVSPPESPRARAPAAQPPTEHGPPPPRPVPGPAEPPEPRVPEPLRKQGRLDPASAVLLRKAKREKAESMLRNDSLSSDQSESVRPSPPKPHRPKRGGKRRQMSVSSSEEEGVSTPEYTSCEDVELESESVSEKGDLDYYWLDPATWHSRETSPISSHPVTWQPSKEGDRLIGRVILNKRTTMPKESGALLGLKVVGGKMTDLGRLGAFITKVKKGSLADVVGHLRAGDEVLEWNGKPLPGATNEEVYNIILESKSEPQVEIIVSRPIGDIPRIPESSHPPLESSSSSFESQKMERPSISVISPTSPGALKDAPQVLPGQLSVKLWYDKVGHQLIVNVLQATDLPPRVDGRPRNPYVKMYFLPDRSDKSKRRTKTVKKLLEPKWNQTFVYSHVHRRDFRERMLEITVWDQPRVQDEESEFLGEILIELETALLDDEPHWYKLQTHDESSLPLPQPSPFMPRRHIHGESSSKKLQRSQRISDSDISDYEVDDGIGVVPPVGYRASARESKATTLTVPEQQRTTHHRSRSVSPHRGDDQGRPRSRLPNVPLQRSLDEIHPTRRSRSPTRHHDASRSPADHRSRHVESQYSSEPDRTHRQGSPTQSPPADTSFGSRRGRQLPQVPVRSGSIEQASLVVEERTRQMKMKVHRFKQTTGSGSSQELDHEQYSKYNIHKDQYRSCDNASAKSSDSDVSDVSAISRASSTSRLSSTSFMSEQSERPRGRISSFTPKMQGRRMGTSGRAIIKSTSVSGEIYTLERNDGSQSDTAVGTVGAGGKKRRSSLSAKVVAIVSRRSRSTSQLSQTESGHKKLKSTIQRSTETGMAAEMRKMVRQPSRESTDGSINSYSSEGNLIFPGVRVGPDSQFSDFLDGLGPAQLVGRQTLATPAMGDIQIGMEDKKGQLEVEVIRARSLTQKPGSKSTPAPYVKVYLLENGACIAKKKTRIARKTLDPLYQQSLVFDESPQGKVLQVIVWGDYGRMDHKCFMGVAQILLEELDLSSMVIGWYKLFPPSSLVDPTLAPLTRRASQSSLESSSGPPCIRS

Target ORF information:

RefSeq Version	XM_017596670.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus regulating synaptic membrane exocytosis 1 (Rims1), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017596670.1

ORF Insert Sequence:

ATGTCCTCGG CCGTGGGGCC CCGAGGTCCT CGCCCACCCA CGGTGCCTCC CCCTATGCAA 
GAACTGCCCG ACCTGAGCCA CCTGACCGAG GAGGAGAGGA ACATTATCAT GGCAGTGATG 
GACCGGCAGA AGGAAGAGGA GGAAAAAGAA GAGGCCATGC TCAAGTGTGT TGTCAGGGAC 
ATGGCGAAGC CTGCTGCCTG CAAAACACCA AGAAATGCTG AAAGCCAGCC CCATCAACCA 
CCACTGAACA TTTTCAGATG TGTCTGTGTT CCCAGAAAGC CAAGCAGCGA AGAGGGAGGC 
CCAGAAAGAG ACTGGAGATT GCATCAACAG TTTGAAAGCT ACAAGGAGCA AGTGAGAAAA 
ATCGGAGAGG AAGCGAGGCG TTACCAGGGC GAGCACAAGG ATGATGCCCC GACGTGTGGA 
ATCTGTCATA AGACAAAGTT TGCTGATGGA TGTGGCCATC TCTGCTCCTA TTGTCGCACC 
AAGTTCTGTG CACGCTGCGG AGGCCGTGTG TCTCTGCGAT CGAACAATGA GGACAAAGTG 
GTTATGTGGG TATGCAATTT ATGTCGAAAG CAACAAGAAA TCTTAACGAA ATCTGGAGCG 
TGGTTCTTTG GAAGTGGCCC TCAGCAGCCT AGTCAAGATG GGACTCTGAG TGACACGGCC 
ACAGGTGCTG GATCTGAGGT GCCAAGAGAA AAGAAAGCAA GGCTCCAAGA GCGATCAAGG 
TCTCAGACGC CCTTGAGTAC AGCAGCTGTC TCTTCCCAAG ACACTGCTAC CCCCGGTGCA 
CCGTTGGACA GGAACAAAGG GGCTGAGCCC TCACAGCAAG CCTTGGGTCC TGAACAGAAG 
CAGGCATCAA GATCAAGAAG CGAGCCACCG AGGGAAAGGA AGAAGGCTCC AGGGCTTTCA 
GAGCAGAATG GCAAGGGAGG CCAGAAGAGC GAGCGCAAAC GTGTCCCCAA GTCTGTGGTG 
CAACCCGGGG AAGGGATCGC GGATGAGAGG GAGAGGAAAG AGAGGCGGGA AACCCGCAGG 
TTGGAGAAAG GGCGCTCCCA GGACTACTCA GACCGGCCTG AGAAACGCGA CAATGGCAGG 
GTGGCGGAAG ACCAGAAGCA GAGGAAGGAG GAGGAGTACC AGACTAGGTA CCGCAGCGAC 
CCTAACCTGG CTCGCTACCC GGTGAAGGCG CCGCCAGAGG AGCAGCAGAT GCGCATGCAC 
GCCCGGGTGT CCCGAGCGAG GCACGAGCGG CGCCACAGCG ACGTGGCGCT CCCGCACACC 
GAGGCAGCTG CCGCCGCGCC GGCTGAGGCC ACGGCGGGCA AGCGCGCGCC GGCCACCGCC 
AGGGTCTCTC CCCCGGAGTC CCCGCGCGCA CGCGCGCCCG CCGCCCAGCC TCCCACCGAG 
CACGGGCCAC CGCCGCCGCG GCCAGTCCCG GGTCCCGCAG AGCCACCCGA GCCGCGCGTC 
CCCGAGCCGC TCCGTAAGCA GGGCCGCCTG GACCCGGCCT CGGCCGTGCT TCTGCGCAAG 
GCCAAGCGCG AGAAGGCGGA GAGCATGCTG CGGAACGACT CGCTGAGCTC CGATCAGTCC 
GAGTCCGTGC GGCCATCCCC GCCCAAGCCT CACCGGCCCA AGCGGGGAGG CAAGAGACGT 
CAGATGTCGG TGAGCAGCTC GGAGGAGGAG GGCGTGTCCA CACCGGAGTA CACGAGCTGC 
GAGGACGTGG AGCTGGAGAG CGAGAGCGTG AGCGAGAAAG GTGACTTGGA TTACTACTGG 
TTGGATCCCG CCACGTGGCA CAGCAGGGAA ACGTCGCCTA TCAGTTCGCA TCCTGTAACG 
TGGCAGCCGT CTAAAGAGGG AGATCGACTA ATCGGCCGTG TTATTCTTAA CAAAAGAACA 
ACCATGCCCA AAGAATCAGG TGCATTATTG GGTCTGAAGG TGGTTGGAGG AAAAATGACG 
GACTTAGGGC GCCTTGGTGC TTTCATCACC AAAGTAAAGA AGGGCAGCCT GGCAGACGTC 
GTCGGACACC TAAGAGCAGG GGACGAAGTC CTAGAGTGGA ATGGTAAACC CCTGCCGGGA 
GCAACAAACG AAGAAGTTTA CAACATTATC TTAGAATCAA AATCAGAACC TCAAGTTGAG 
ATTATTGTTT CAAGGCCTAT TGGTGACATC CCCAGGATCC CTGAGAGTTC CCATCCTCCC 
CTGGAGTCCA GTTCAAGTTC CTTTGAATCT CAGAAAATGG AAAGGCCTTC TATTTCTGTT 
ATTTCTCCAA CCAGCCCTGG AGCTCTGAAA GATGCCCCAC AAGTCTTACC AGGGCAACTC 
TCAGTGAAGC TATGGTATGA TAAAGTGGGG CACCAGCTGA TTGTAAATGT TCTACAAGCA 
ACAGATCTAC CCCCTAGAGT AGATGGCCGT CCCAGGAATC CCTATGTAAA AATGTATTTT 
CTTCCAGATA GAAGCGACAA AAGTAAAAGG AGAACCAAAA CAGTAAAGAA ACTTCTAGAG 
CCAAAATGGA ACCAGACATT TGTCTACTCA CACGTACATC GTAGAGATTT TCGAGAGCGA 
ATGTTAGAGA TTACCGTGTG GGACCAGCCG AGAGTACAGG ACGAAGAGAG TGAATTTCTT 
GGAGAGATCC TCATAGAGTT GGAAACAGCG CTTTTAGATG ATGAGCCCCA TTGGTATAAA 
CTCCAGACAC ATGACGAATC TTCACTACCT CTGCCTCAGC CATCACCGTT CATGCCCAGG 
CGGCATATTC ATGGAGAGAG CTCCAGCAAA AAGCTACAAA GATCTCAGCG AATCAGTGAT 
AGTGACATCT CAGATTATGA GGTTGATGAT GGTATTGGAG TAGTGCCTCC AGTGGGTTAT 
AGAGCTAGTG CTAGAGAGAG TAAAGCAACC ACGTTAACAG TGCCAGAGCA ACAAAGAACT 
ACACATCACC GCTCACGTTC CGTGTCTCCT CATCGCGGCG ATGATCAGGG AAGGCCTCGT 
TCACGTTTAC CAAATGTGCC ATTACAGAGG AGCTTAGATG AAATTCATCC AACACGAAGG 
TCACGTTCTC CAACCCGACA CCATGATGCC TCCCGAAGCC CGGCCGATCA CAGATCCAGA 
CATGTGGAAA GTCAATATTC GTCAGAGCCA GACAGAACGC ACCGACAAGG AAGCCCAACC 
CAGTCTCCTC CAGCAGACAC ATCCTTCGGC AGTCGCCGTG GAAGACAGCT CCCACAGGTG 
CCAGTTCGAA GCGGCAGTAT AGAACAAGCA AGCTTAGTAG TGGAGGAGCG AACGAGACAG 
ATGAAAATGA AAGTTCACCG ATTTAAGCAG ACAACAGGGT CTGGGTCTAG TCAAGAACTT 
GACCACGAGC AATACTCCAA GTACAACATA CATAAAGATC AGTACAGAAG CTGTGATAAC 
GCGTCTGCCA AGTCTTCAGA TAGTGATGTC AGTGATGTGT CCGCCATTTC CAGAGCCAGC 
AGTACCTCAC GCCTCAGCAG CACAAGCTTT ATGTCAGAGC AGTCTGAGCG CCCCAGGGGT 
AGGATCAGTT CATTTACCCC CAAAATGCAA GGCAGACGGA TGGGGACTTC AGGAAGAGCC 
ATCATCAAGA GCACCAGTGT AAGTGGAGAG ATATATACAC TGGAACGTAA TGACGGTAGC 
CAGTCGGACA CGGCCGTGGG TACCGTCGGA GCCGGTGGAA AGAAACGAAG ATCCAGCCTG 
AGCGCCAAAG TGGTAGCCAT TGTGTCTCGA AGAAGCAGGA GCACGTCACA GCTCAGCCAG 
ACAGAGTCGG GCCACAAGAA GTTGAAAAGC ACCATCCAGA GGAGTACGGA AACAGGAATG 
GCAGCTGAAA TGCGGAAGAT GGTGAGACAG CCGAGCCGGG AGTCCACGGA TGGCAGCATC 
AACAGTTATA GCTCGGAAGG AAACTTGATA TTTCCTGGAG TTCGAGTAGG ACCCGACAGT 
CAGTTCAGTG ATTTCCTTGA TGGGTTGGGA CCAGCGCAGC TCGTTGGCCG TCAGACGCTC 
GCCACCCCGG CCATGGGCGA TATCCAAATC GGGATGGAGG ATAAGAAGGG TCAGTTGGAG 
GTTGAGGTTA TCAGAGCCCG GAGCCTTACA CAAAAACCTG GTTCCAAATC TACACCCGCT 
CCCTATGTGA AAGTATATCT TTTGGAAAAT GGAGCCTGTA TTGCCAAAAA GAAGACAAGA 
ATTGCACGGA AAACTCTCGA TCCTTTGTAT CAGCAGTCCC TGGTTTTTGA TGAAAGTCCA 
CAGGGTAAAG TTCTTCAGGT GATTGTCTGG GGTGACTATG GAAGAATGGA CCACAAATGC 
TTTATGGGTG TGGCTCAAAT CTTGTTGGAA GAACTTGATC TATCCAGCAT GGTGATTGGA 
TGGTATAAAT TGTTCCCTCC GTCCTCACTG GTGGATCCCA CTCTCGCTCC CCTGACCCGC 
CGGGCTTCCC AATCATCTCT GGAAAGTTCG TCCGGGCCTC CCTGCATCCG GTCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rims1 ( XM_017596671.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017452160.1 Rattus norvegicus regulating synaptic membrane exocytosis 1 (Rims1), transcript variant X18, mRNA.

Price & Availability↑

CloneID	ORa62888
Clone ID Related Accession (Same CDS sequence)	XM_017596671.1
Accession Version	XM_017596671.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4413bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	regulating synaptic membrane exocytosis protein 1 isoform X18
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005108.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCTCGG CCGTGGGGCC CCGAGGTCCT CGCCCACCCA CGGTGCCTCC CCCTATGCAA 
GAACTGCCCG ACCTGAGCCA CCTGACCGAG GAGGAGAGGA ACATTATCAT GGCAGTGATG 
GACCGGCAGA AGGAAGAGGA GGAAAAAGAA GAGGCCATGC TCAAGTGTGT TGTCAGGGAC 
ATGGCGAAGC CTGCTGCCTG CAAAACACCA AGAAATGCTG AAAGCCAGCC CCATCAACCA 
CCACTGAACA TTTTCAGATG TGTCTGTGTT CCCAGAAAGC CAAGCAGCGA AGAGGGAGGC 
CCAGAAAGAG ACTGGAGATT GCATCAACAG TTTGAAAGCT ACAAGGAGCA AGTGAGAAAA 
ATCGGAGAGG AAGCGAGGCG TTACCAGGGC GAGCACAAGG ATGATGCCCC GACGTGTGGA 
ATCTGTCATA AGACAAAGTT TGCTGATGGA TGTGGCCATC TCTGCTCCTA TTGTCGCACC 
AAGTTCTGTG CACGCTGCGG AGGCCGTGTG TCTCTGCGAT CGAACAATGA GGACAAAGTG 
GTTATGTGGG TATGCAATTT ATGTCGAAAG CAACAAGAAA TCTTAACGAA ATCTGGAGCG 
TGGTTCTTTG GAAGTGGCCC TCAGCAGCCT AGTCAAGATG GGACTCTGAG TGACACGGCC 
ACAGGTGCTG GATCTGAGGT GCCAAGAGAA AAGAAAGCAA GGCTCCAAGA GCGATCAAGG 
TCTCAGACGC CCTTGAGTAC AGCAGCTGTC TCTTCCCAAG ACACTGCTAC CCCCGGTGCA 
CCGTTGGACA GGAACAAAGG GGCTGAGCCC TCACAGCAAG CCTTGGGTCC TGAACAGAAG 
CAGGCATCAA GATCAAGAAG CGAGCCACCG AGGGAAAGGA AGAAGGCTCC AGGGCTTTCA 
GAGCAGAATG GCAAGGGAGG CCAGAAGAGC GAGCGCAAAC GTGTCCCCAA GTCTGTGGTG 
CAACCCGGGG AAGGGATCGC GGATGAGAGG GAGAGGAAAG AGAGGCGGGA AACCCGCAGG 
TTGGAGAAAG GGCGCTCCCA GGACTACTCA GACCGGCCTG AGAAACGCGA CAATGGCAGG 
GTGGCGGAAG ACCAGAAGCA GAGGAAGGAG GAGGAGTACC AGACTAGGTA CCGCAGCGAC 
CCTAACCTGG CTCGCTACCC GGTGAAGGCG CCGCCAGAGG AGCAGCAGAT GCGCATGCAC 
GCCCGGGTGT CCCGAGCGAG GCACGAGCGG CGCCACAGCG ACGTGGCGCT CCCGCACACC 
GAGGCAGCTG CCGCCGCGCC GGCTGAGGCC ACGGCGGGCA AGCGCGCGCC GGCCACCGCC 
AGGGTCTCTC CCCCGGAGTC CCCGCGCGCA CGCGCGCCCG CCGCCCAGCC TCCCACCGAG 
CACGGGCCAC CGCCGCCGCG GCCAGTCCCG GGTCCCGCAG AGCCACCCGA GCCGCGCGTC 
CCCGAGCCGC TCCGTAAGCA GGGCCGCCTG GACCCGGCCT CGGCCGTGCT TCTGCGCAAG 
GCCAAGCGCG AGAAGGCGGA GAGCATGCTG CGGAACGACT CGCTGAGCTC CGATCAGTCC 
GAGTCCGTGC GGCCATCCCC GCCCAAGCCT CACCGGCCCA AGCGGGGAGG CAAGAGACGT 
CAGATGTCGG TGAGCAGCTC GGAGGAGGAG GGCGTGTCCA CACCGGAGTA CACGAGCTGC 
GAGGACGTGG AGCTGGAGAG CGAGAGCGTG AGCGAGAAAG GTGACTTGGA TTACTACTGG 
TTGGATCCCG CCACGTGGCA CAGCAGGGAA ACGTCGCCTA TCAGTTCGCA TCCTGTAACG 
TGGCAGCCGT CTAAAGAGGG AGATCGACTA ATCGGCCGTG TTATTCTTAA CAAAAGAACA 
ACCATGCCCA AAGAATCAGG TGCATTATTG GGTCTGAAGG TGGTTGGAGG AAAAATGACG 
GACTTAGGGC GCCTTGGTGC TTTCATCACC AAAGTAAAGA AGGGCAGCCT GGCAGACGTC 
GTCGGACACC TAAGAGCAGG GGACGAAGTC CTAGAGTGGA ATGGTAAACC CCTGCCGGGA 
GCAACAAACG AAGAAGTTTA CAACATTATC TTAGAATCAA AATCAGAACC TCAAGTTGAG 
ATTATTGTTT CAAGGCCTAT TGGTGACATC CCCAGGATCC CTGAGAGTTC CCATCCTCCC 
CTGGAGTCCA GTTCAAGTTC CTTTGAATCT CAGAAAATGG AAAGGCCTTC TATTTCTGTT 
ATTTCTCCAA CCAGCCCTGG AGCTCTGAAA GATGCCCCAC AAGTCTTACC AGGGCAACTC 
TCAGTGAAGC TATGGTATGA TAAAGTGGGG CACCAGCTGA TTGTAAATGT TCTACAAGCA 
ACAGATCTAC CCCCTAGAGT AGATGGCCGT CCCAGGAATC CCTATGTAAA AATGTATTTT 
CTTCCAGATA GAAGCGACAA AAGTAAAAGG AGAACCAAAA CAGTAAAGAA ACTTCTAGAG 
CCAAAATGGA ACCAGACATT TGTCTACTCA CACGTACATC GTAGAGATTT TCGAGAGCGA 
ATGTTAGAGA TTACCGTGTG GGACCAGCCG AGAGTACAGG ACGAAGAGAG TGAATTTCTT 
GGAGAGATCC TCATAGAGTT GGAAACAGCG CTTTTAGATG ATGAGCCCCA TTGGTATAAA 
CTCCAGACAC ATGACGAATC TTCACTACCT CTGCCTCAGC CATCACCGTT CATGCCCAGG 
CGGCATATTC ATGGAGAGAG CTCCAGCAAA AAGCTACAAA GATCTCAGCG AATCAGTGAT 
AGTGACATCT CAGATTATGA GGTTGATGAT GGTATTGGAG TAGTGCCTCC AGTGGGTTAT 
AGAGCTAGTG CTAGAGAGAG TAAAGCAACC ACGTTAACAG TGCCAGAGCA ACAAAGAACT 
ACACATCACC GCTCACGTTC CGTGTCTCCT CATCGCGGCG ATGATCAGGG AAGGCCTCGT 
TCACGTTTAC CAAATGTGCC ATTACAGAGG AGCTTAGATG AAATTCATCC AACACGAAGG 
TCACGTTCTC CAACCCGACA CCATGATGCC TCCCGAAGCC CGGCCGATCA CAGATCCAGA 
CATGTGGAAA GTCAATATTC GTCAGAGCCA GACAGTGAGC TTCTCATGCT GCCCAGAGCA 
AAACGAGGAC GAAGTGCAGA AAGCCTACAC ATGACCAGTG AACTGCAGCC CTCTCTTGAC 
AGGGCTAGGA GTGCTAGTAC CAACTGCTTG AGACCAGATA CTAGTTTGCA TTCACCAGAA 
CGAGAAAGGG GTAGATGGTC CCCCTCCCTA GATAGGAGGC GACCTGCTAG CCCCAGGATT 
CAAATCCAGC ATGCATCTCC GGAGAATGAC AGGCACTCCA GAAAGTCTGA AAGATGTAGC 
ATCCAAAAAC AGTCTAGGAA AGGCACAGCC TCTGATGCAG ACAGGGTTCT CCCACCATGC 
CTTTCTAGAA GGGGATACGC AACCCCAAGA GCAACCGATC AACCGGTCGT TAGGGGAAAG 
CATCCCACTC GTTCACGGTC GAGCGAGCAC TCTAGTGTCA GAACCCTGTG TTCTATGCAC 
CACCTTGCCC CCGGAGGGTC GGCGCCACCT TCTCCACTTC TGACAAGAAC GCACCGACAA 
GGAAGCCCAA CCCAGTCTCC TCCAGCAGAC ACATCCTTCG GCAGTCGCCG TGGAAGACAG 
CTCCCACAGG TGCCAGTTCG AAGCGGCAGT ATAGAACAAG AGTCGGGCCA CAAGAAGTTG 
AAAAGCACCA TCCAGAGGAG TACGGAAACA GGAATGGCAG CTGAAATGCG GAAGATGGTG 
AGACAGCCGA GCCGGGAGTC CACGGATGGC AGCATCAACA GTTATAGCTC GGAAGGAAAC 
TTGATATTTC CTGGAGTTCG AGTAGGACCC GACAGTCAGT TCAGTGATTT CCTTGATGGG 
TTGGGACCAG CGCAGCTCGT TGGCCGTCAG ACGCTCGCCA CCCCGGCCAT GGGCGATATC 
CAAATCGGGA TGGAGGATAA GAAGGGTCAG TTGGAGGTTG AGGTTATCAG AGCCCGGAGC 
CTTACACAAA AACCTGGTTC CAAATCTACA CCCGCTCCCT ATGTGAAAGT ATATCTTTTG 
GAAAATGGAG CCTGTATTGC CAAAAAGAAG ACAAGAATTG CACGGAAAAC TCTCGATCCT 
TTGTATCAGC AGTCCCTGGT TTTTGATGAA AGTCCACAGG GTAAAGTTCT TCAGGTGATT 
GTCTGGGGTG ACTATGGAAG AATGGACCAC AAATGCTTTA TGGGTGTGGC TCAAATCTTG 
TTGGAAGAAC TTGATCTATC CAGCATGGTG ATTGGATGGT ATAAATTGTT CCCTCCGTCC 
TCACTGGTGG ATCCCACTCT CGCTCCCCTG ACCCGCCGGG CTTCCCAATC ATCTCTGGAA 
AGTTCGTCCG GGCCTCCCTG CATCCGGTCA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017452160.1
CDS	359..4771
Translation	MSSAVGPRGPRPPTVPPPMQELPDLSHLTEEERNIIMAVMDRQKEEEEKEEAMLKCVVRDMAKPAACKTPRNAESQPHQPPLNIFRCVCVPRKPSSEEGGPERDWRLHQQFESYKEQVRKIGEEARRYQGEHKDDAPTCGICHKTKFADGCGHLCSYCRTKFCARCGGRVSLRSNNEDKVVMWVCNLCRKQQEILTKSGAWFFGSGPQQPSQDGTLSDTATGAGSEVPREKKARLQERSRSQTPLSTAAVSSQDTATPGAPLDRNKGAEPSQQALGPEQKQASRSRSEPPRERKKAPGLSEQNGKGGQKSERKRVPKSVVQPGEGIADERERKERRETRRLEKGRSQDYSDRPEKRDNGRVAEDQKQRKEEEYQTRYRSDPNLARYPVKAPPEEQQMRMHARVSRARHERRHSDVALPHTEAAAAAPAEATAGKRAPATARVSPPESPRARAPAAQPPTEHGPPPPRPVPGPAEPPEPRVPEPLRKQGRLDPASAVLLRKAKREKAESMLRNDSLSSDQSESVRPSPPKPHRPKRGGKRRQMSVSSSEEEGVSTPEYTSCEDVELESESVSEKGDLDYYWLDPATWHSRETSPISSHPVTWQPSKEGDRLIGRVILNKRTTMPKESGALLGLKVVGGKMTDLGRLGAFITKVKKGSLADVVGHLRAGDEVLEWNGKPLPGATNEEVYNIILESKSEPQVEIIVSRPIGDIPRIPESSHPPLESSSSSFESQKMERPSISVISPTSPGALKDAPQVLPGQLSVKLWYDKVGHQLIVNVLQATDLPPRVDGRPRNPYVKMYFLPDRSDKSKRRTKTVKKLLEPKWNQTFVYSHVHRRDFRERMLEITVWDQPRVQDEESEFLGEILIELETALLDDEPHWYKLQTHDESSLPLPQPSPFMPRRHIHGESSSKKLQRSQRISDSDISDYEVDDGIGVVPPVGYRASARESKATTLTVPEQQRTTHHRSRSVSPHRGDDQGRPRSRLPNVPLQRSLDEIHPTRRSRSPTRHHDASRSPADHRSRHVESQYSSEPDSELLMLPRAKRGRSAESLHMTSELQPSLDRARSASTNCLRPDTSLHSPERERGRWSPSLDRRRPASPRIQIQHASPENDRHSRKSERCSIQKQSRKGTASDADRVLPPCLSRRGYATPRATDQPVVRGKHPTRSRSSEHSSVRTLCSMHHLAPGGSAPPSPLLTRTHRQGSPTQSPPADTSFGSRRGRQLPQVPVRSGSIEQESGHKKLKSTIQRSTETGMAAEMRKMVRQPSRESTDGSINSYSSEGNLIFPGVRVGPDSQFSDFLDGLGPAQLVGRQTLATPAMGDIQIGMEDKKGQLEVEVIRARSLTQKPGSKSTPAPYVKVYLLENGACIAKKKTRIARKTLDPLYQQSLVFDESPQGKVLQVIVWGDYGRMDHKCFMGVAQILLEELDLSSMVIGWYKLFPPSSLVDPTLAPLTRRASQSSLESSSGPPCIRS

Target ORF information:

RefSeq Version	XM_017596671.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus regulating synaptic membrane exocytosis 1 (Rims1), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017596671.1

ORF Insert Sequence:

ATGTCCTCGG CCGTGGGGCC CCGAGGTCCT CGCCCACCCA CGGTGCCTCC CCCTATGCAA 
GAACTGCCCG ACCTGAGCCA CCTGACCGAG GAGGAGAGGA ACATTATCAT GGCAGTGATG 
GACCGGCAGA AGGAAGAGGA GGAAAAAGAA GAGGCCATGC TCAAGTGTGT TGTCAGGGAC 
ATGGCGAAGC CTGCTGCCTG CAAAACACCA AGAAATGCTG AAAGCCAGCC CCATCAACCA 
CCACTGAACA TTTTCAGATG TGTCTGTGTT CCCAGAAAGC CAAGCAGCGA AGAGGGAGGC 
CCAGAAAGAG ACTGGAGATT GCATCAACAG TTTGAAAGCT ACAAGGAGCA AGTGAGAAAA 
ATCGGAGAGG AAGCGAGGCG TTACCAGGGC GAGCACAAGG ATGATGCCCC GACGTGTGGA 
ATCTGTCATA AGACAAAGTT TGCTGATGGA TGTGGCCATC TCTGCTCCTA TTGTCGCACC 
AAGTTCTGTG CACGCTGCGG AGGCCGTGTG TCTCTGCGAT CGAACAATGA GGACAAAGTG 
GTTATGTGGG TATGCAATTT ATGTCGAAAG CAACAAGAAA TCTTAACGAA ATCTGGAGCG 
TGGTTCTTTG GAAGTGGCCC TCAGCAGCCT AGTCAAGATG GGACTCTGAG TGACACGGCC 
ACAGGTGCTG GATCTGAGGT GCCAAGAGAA AAGAAAGCAA GGCTCCAAGA GCGATCAAGG 
TCTCAGACGC CCTTGAGTAC AGCAGCTGTC TCTTCCCAAG ACACTGCTAC CCCCGGTGCA 
CCGTTGGACA GGAACAAAGG GGCTGAGCCC TCACAGCAAG CCTTGGGTCC TGAACAGAAG 
CAGGCATCAA GATCAAGAAG CGAGCCACCG AGGGAAAGGA AGAAGGCTCC AGGGCTTTCA 
GAGCAGAATG GCAAGGGAGG CCAGAAGAGC GAGCGCAAAC GTGTCCCCAA GTCTGTGGTG 
CAACCCGGGG AAGGGATCGC GGATGAGAGG GAGAGGAAAG AGAGGCGGGA AACCCGCAGG 
TTGGAGAAAG GGCGCTCCCA GGACTACTCA GACCGGCCTG AGAAACGCGA CAATGGCAGG 
GTGGCGGAAG ACCAGAAGCA GAGGAAGGAG GAGGAGTACC AGACTAGGTA CCGCAGCGAC 
CCTAACCTGG CTCGCTACCC GGTGAAGGCG CCGCCAGAGG AGCAGCAGAT GCGCATGCAC 
GCCCGGGTGT CCCGAGCGAG GCACGAGCGG CGCCACAGCG ACGTGGCGCT CCCGCACACC 
GAGGCAGCTG CCGCCGCGCC GGCTGAGGCC ACGGCGGGCA AGCGCGCGCC GGCCACCGCC 
AGGGTCTCTC CCCCGGAGTC CCCGCGCGCA CGCGCGCCCG CCGCCCAGCC TCCCACCGAG 
CACGGGCCAC CGCCGCCGCG GCCAGTCCCG GGTCCCGCAG AGCCACCCGA GCCGCGCGTC 
CCCGAGCCGC TCCGTAAGCA GGGCCGCCTG GACCCGGCCT CGGCCGTGCT TCTGCGCAAG 
GCCAAGCGCG AGAAGGCGGA GAGCATGCTG CGGAACGACT CGCTGAGCTC CGATCAGTCC 
GAGTCCGTGC GGCCATCCCC GCCCAAGCCT CACCGGCCCA AGCGGGGAGG CAAGAGACGT 
CAGATGTCGG TGAGCAGCTC GGAGGAGGAG GGCGTGTCCA CACCGGAGTA CACGAGCTGC 
GAGGACGTGG AGCTGGAGAG CGAGAGCGTG AGCGAGAAAG GTGACTTGGA TTACTACTGG 
TTGGATCCCG CCACGTGGCA CAGCAGGGAA ACGTCGCCTA TCAGTTCGCA TCCTGTAACG 
TGGCAGCCGT CTAAAGAGGG AGATCGACTA ATCGGCCGTG TTATTCTTAA CAAAAGAACA 
ACCATGCCCA AAGAATCAGG TGCATTATTG GGTCTGAAGG TGGTTGGAGG AAAAATGACG 
GACTTAGGGC GCCTTGGTGC TTTCATCACC AAAGTAAAGA AGGGCAGCCT GGCAGACGTC 
GTCGGACACC TAAGAGCAGG GGACGAAGTC CTAGAGTGGA ATGGTAAACC CCTGCCGGGA 
GCAACAAACG AAGAAGTTTA CAACATTATC TTAGAATCAA AATCAGAACC TCAAGTTGAG 
ATTATTGTTT CAAGGCCTAT TGGTGACATC CCCAGGATCC CTGAGAGTTC CCATCCTCCC 
CTGGAGTCCA GTTCAAGTTC CTTTGAATCT CAGAAAATGG AAAGGCCTTC TATTTCTGTT 
ATTTCTCCAA CCAGCCCTGG AGCTCTGAAA GATGCCCCAC AAGTCTTACC AGGGCAACTC 
TCAGTGAAGC TATGGTATGA TAAAGTGGGG CACCAGCTGA TTGTAAATGT TCTACAAGCA 
ACAGATCTAC CCCCTAGAGT AGATGGCCGT CCCAGGAATC CCTATGTAAA AATGTATTTT 
CTTCCAGATA GAAGCGACAA AAGTAAAAGG AGAACCAAAA CAGTAAAGAA ACTTCTAGAG 
CCAAAATGGA ACCAGACATT TGTCTACTCA CACGTACATC GTAGAGATTT TCGAGAGCGA 
ATGTTAGAGA TTACCGTGTG GGACCAGCCG AGAGTACAGG ACGAAGAGAG TGAATTTCTT 
GGAGAGATCC TCATAGAGTT GGAAACAGCG CTTTTAGATG ATGAGCCCCA TTGGTATAAA 
CTCCAGACAC ATGACGAATC TTCACTACCT CTGCCTCAGC CATCACCGTT CATGCCCAGG 
CGGCATATTC ATGGAGAGAG CTCCAGCAAA AAGCTACAAA GATCTCAGCG AATCAGTGAT 
AGTGACATCT CAGATTATGA GGTTGATGAT GGTATTGGAG TAGTGCCTCC AGTGGGTTAT 
AGAGCTAGTG CTAGAGAGAG TAAAGCAACC ACGTTAACAG TGCCAGAGCA ACAAAGAACT 
ACACATCACC GCTCACGTTC CGTGTCTCCT CATCGCGGCG ATGATCAGGG AAGGCCTCGT 
TCACGTTTAC CAAATGTGCC ATTACAGAGG AGCTTAGATG AAATTCATCC AACACGAAGG 
TCACGTTCTC CAACCCGACA CCATGATGCC TCCCGAAGCC CGGCCGATCA CAGATCCAGA 
CATGTGGAAA GTCAATATTC GTCAGAGCCA GACAGTGAGC TTCTCATGCT GCCCAGAGCA 
AAACGAGGAC GAAGTGCAGA AAGCCTACAC ATGACCAGTG AACTGCAGCC CTCTCTTGAC 
AGGGCTAGGA GTGCTAGTAC CAACTGCTTG AGACCAGATA CTAGTTTGCA TTCACCAGAA 
CGAGAAAGGG GTAGATGGTC CCCCTCCCTA GATAGGAGGC GACCTGCTAG CCCCAGGATT 
CAAATCCAGC ATGCATCTCC GGAGAATGAC AGGCACTCCA GAAAGTCTGA AAGATGTAGC 
ATCCAAAAAC AGTCTAGGAA AGGCACAGCC TCTGATGCAG ACAGGGTTCT CCCACCATGC 
CTTTCTAGAA GGGGATACGC AACCCCAAGA GCAACCGATC AACCGGTCGT TAGGGGAAAG 
CATCCCACTC GTTCACGGTC GAGCGAGCAC TCTAGTGTCA GAACCCTGTG TTCTATGCAC 
CACCTTGCCC CCGGAGGGTC GGCGCCACCT TCTCCACTTC TGACAAGAAC GCACCGACAA 
GGAAGCCCAA CCCAGTCTCC TCCAGCAGAC ACATCCTTCG GCAGTCGCCG TGGAAGACAG 
CTCCCACAGG TGCCAGTTCG AAGCGGCAGT ATAGAACAAG AGTCGGGCCA CAAGAAGTTG 
AAAAGCACCA TCCAGAGGAG TACGGAAACA GGAATGGCAG CTGAAATGCG GAAGATGGTG 
AGACAGCCGA GCCGGGAGTC CACGGATGGC AGCATCAACA GTTATAGCTC GGAAGGAAAC 
TTGATATTTC CTGGAGTTCG AGTAGGACCC GACAGTCAGT TCAGTGATTT CCTTGATGGG 
TTGGGACCAG CGCAGCTCGT TGGCCGTCAG ACGCTCGCCA CCCCGGCCAT GGGCGATATC 
CAAATCGGGA TGGAGGATAA GAAGGGTCAG TTGGAGGTTG AGGTTATCAG AGCCCGGAGC 
CTTACACAAA AACCTGGTTC CAAATCTACA CCCGCTCCCT ATGTGAAAGT ATATCTTTTG 
GAAAATGGAG CCTGTATTGC CAAAAAGAAG ACAAGAATTG CACGGAAAAC TCTCGATCCT 
TTGTATCAGC AGTCCCTGGT TTTTGATGAA AGTCCACAGG GTAAAGTTCT TCAGGTGATT 
GTCTGGGGTG ACTATGGAAG AATGGACCAC AAATGCTTTA TGGGTGTGGC TCAAATCTTG 
TTGGAAGAAC TTGATCTATC CAGCATGGTG ATTGGATGGT ATAAATTGTT CCCTCCGTCC 
TCACTGGTGG ATCCCACTCT CGCTCCCCTG ACCCGCCGGG CTTCCCAATC ATCTCTGGAA 
AGTTCGTCCG GGCCTCCCTG CATCCGGTCA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rims1 ( XM_017596672.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017452161.1 Rattus norvegicus regulating synaptic membrane exocytosis 1 (Rims1), transcript variant X19, mRNA.

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CloneID	ORa62889
Clone ID Related Accession (Same CDS sequence)	XM_017596672.1
Accession Version	XM_017596672.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4146bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	regulating synaptic membrane exocytosis protein 1 isoform X19
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005108.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCTCGG CCGTGGGGCC CCGAGGTCCT CGCCCACCCA CGGTGCCTCC CCCTATGCAA 
GAACTGCCCG ACCTGAGCCA CCTGACCGAG GAGGAGAGGA ACATTATCAT GGCAGTGATG 
GACCGGCAGA AGGAAGAGGA GGAAAAAGAA GAGGCCATGC TCAAGTGTGT TGTCAGGGAC 
ATGGCGAAGC CTGCTGCCTG CAAAACACCA AGAAATGCTG AAAGCCAGCC CCATCAACCA 
CCACTGAACA TTTTCAGATG TGTCTGTGTT CCCAGAAAGC CAAGCAGCGA AGAGGGAGGC 
CCAGAAAGAG ACTGGAGATT GCATCAACAG TTTGAAAGCT ACAAGGAGCA AGTGAGAAAA 
ATCGGAGAGG AAGCGAGGCG TTACCAGGGC GAGCACAAGG ATGATGCCCC GACGTGTGGA 
ATCTGTCATA AGACAAAGTT TGCTGATGGA TGTGGCCATC TCTGCTCCTA TTGTCGCACC 
AAGTTCTGTG CACGCTGCGG AGGCCGTGTG TCTCTGCGAT CGAACAATGA GGACAAAGTG 
GTTATGTGGG TATGCAATTT ATGTCGAAAG CAACAAGAAA TCTTAACGAA ATCTGGAGCG 
TGGTTCTTTG GAAGTGGCCC TCAGCAGCCT AGTCAAGATG GGACTCTGAG TGACACGGCC 
ACAGGTGCTG GATCTGAGGT GCCAAGAGAA AAGAAAGCAA GGCTCCAAGA GCGATCAAGG 
TCTCAGACGC CCTTGAGTAC AGCAGCTGTC TCTTCCCAAG ACACTGCTAC CCCCGGTGCA 
CCGTTGGACA GGAACAAAGG GGCTGAGCCC TCACAGCAAG CCTTGGGTCC TGAACAGAAG 
CAGGCATCAA GATCAAGAAG CGAGCCACCG AGGGAAAGGA AGAAGGCTCC AGGGCTTTCA 
GAGCAGAATG GCAAGGGAGG CCAGAAGAGC GAGCGCAAAC GTGTCCCCAA GTCTGTGGTG 
CAACCCGGGG AAGGGATCGC GGATGAGAGG GAGAGGAAAG AGAGGCGGGA AACCCGCAGG 
TTGGAGAAAG GGCGCTCCCA GGACTACTCA GACCGGCCTG AGAAACGCGA CAATGGCAGG 
GTGGCGGAAG ACCAGAAGCA GAGGAAGGAG GAGGAGTACC AGACTAGGTA CCGCAGCGAC 
CCTAACCTGG CTCGCTACCC GGTGAAGGCG CCGCCAGAGG AGCAGCAGAT GCGCATGCAC 
GCCCGGGTGT CCCGAGCGAG GCACGAGCGG CGCCACAGCG ACGTGGCGCT CCCGCACACC 
GAGGCAGCTG CCGCCGCGCC GGCTGAGGCC ACGGCGGGCA AGCGCGCGCC GGCCACCGCC 
AGGGTCTCTC CCCCGGAGTC CCCGCGCGCA CGCGCGCCCG CCGCCCAGCC TCCCACCGAG 
CACGGGCCAC CGCCGCCGCG GCCAGTCCCG GGTCCCGCAG AGCCACCCGA GCCGCGCGTC 
CCCGAGCCGC TCCGTAAGCA GGGCCGCCTG GACCCGGCCT CGGCCGTGCT TCTGCGCAAG 
GCCAAGCGCG AGAAGGCGGA GAGCATGCTG CGGAACGACT CGCTGAGCTC CGATCAGTCC 
GAGTCCGTGC GGCCATCCCC GCCCAAGCCT CACCGGCCCA AGCGGGGAGG CAAGAGACGT 
CAGATGTCGG TGAGCAGCTC GGAGGAGGAG GGCGTGTCCA CACCGGAGTA CACGAGCTGC 
GAGGACGTGG AGCTGGAGAG CGAGAGCGTG AGCGAGAAAG GTGACTTGGA TTACTACTGG 
TTGGATCCCG CCACGTGGCA CAGCAGGGAA ACGTCGCCTA TCAGTTCGCA TCCTGTAACG 
TGGCAGCCGT CTAAAGAGGG AGATCGACTA ATCGGCCGTG TTATTCTTAA CAAAAGAACA 
ACCATGCCCA AAGAATCAGG TGCATTATTG GGTCTGAAGG TGGTTGGAGG AAAAATGACG 
GACTTAGGGC GCCTTGGTGC TTTCATCACC AAAGTAAAGA AGGGCAGCCT GGCAGACGTC 
GTCGGACACC TAAGAGCAGG GGACGAAGTC CTAGAGTGGA ATGGTAAACC CCTGCCGGGA 
GCAACAAACG AAGAAGTTTA CAACATTATC TTAGAATCAA AATCAGAACC TCAAGTTGAG 
ATTATTGTTT CAAGGCCTAT TGGTGACATC CCCAGGATCC CTGAGAGTTC CCATCCTCCC 
CTGGAGTCCA GTTCAAGTTC CTTTGAATCT CAGAAAATGG AAAGGCCTTC TATTTCTGTT 
ATTTCTCCAA CCAGCCCTGG AGCTCTGAAA GATGCCCCAC AAGTCTTACC AGGGCAACTC 
TCAGTGAAGC TATGGTATGA TAAAGTGGGG CACCAGCTGA TTGTAAATGT TCTACAAGCA 
ACAGATCTAC CCCCTAGAGT AGATGGCCGT CCCAGGAATC CCTATGTAAA AATGTATTTT 
CTTCCAGATA GAAGCGACAA AAGTAAAAGG AGAACCAAAA CAGTAAAGAA ACTTCTAGAG 
CCAAAATGGA ACCAGACATT TGTCTACTCA CACGTACATC GTAGAGATTT TCGAGAGCGA 
ATGTTAGAGA TTACCGTGTG GGACCAGCCG AGAGTACAGG ACGAAGAGAG TGAATTTCTT 
GGAGAGATCC TCATAGAGTT GGAAACAGCG CTTTTAGATG ATGAGCCCCA TTGGTATAAA 
CTCCAGACAC ATGACGAATC TTCACTACCT CTGCCTCAGC CATCACCGTT CATGCCCAGG 
CGGCATATTC ATGGAGAGAG CTCCAGCAAA AAGCTACAAA GATCTCAGCG AATCAGTGAT 
AGTGACATCT CAGATTATGA GGTTGATGAT GGTATTGGAG TAGTGCCTCC AGTGGGTTAT 
AGAGCTAGTG CTAGAGAGAG TAAAGCAACC ACGTTAACAG TGCCAGAGCA ACAAAGAACT 
ACACATCACC GCTCACGTTC CGTGTCTCCT CATCGCGGCG ATGATCAGGG AAGGCCTCGT 
TCACGTTTAC CAAATGTGCC ATTACAGAGG AGCTTAGATG AAATTCATCC AACACGAAGG 
TCACGTTCTC CAACCCGACA CCATGATGCC TCCCGAAGCC CGGCCGATCA CAGATCCAGA 
CATGTGGAAA GTCAATATTC GTCAGAGCCA GACAGTGAGC TTCTCATGCT GCCCAGAGCA 
AAACGAGGAC GAAGTGCAGA AAGCCTACAC ATGACCAGTG AACTGCAGCC CTCTCTTGAC 
AGGGCTAGGA GTGCTAGTAC CAACTGCTTG AGACCAGATA CTAGTTTGCA TTCACCAGAA 
CGAGAAAGGC ACTCCAGAAA GTCTGAAAGA TGTAGCATCC AAAAACAGTC TAGGAAAGGC 
ACAGCCTCTG ATGCAGACAG AACGCACCGA CAAGGAAGCC CAACCCAGTC TCCTCCAGCA 
GACACATCCT TCGGCAGTCG CCGTGGAAGA CAGCTCCCAC AGGTGCCAGT TCGAAGCGGC 
AGTATAGAAC AAGAGTCGGG CCACAAGAAG TTGAAAAGCA CCATCCAGAG GAGTACGGAA 
ACAGGAATGG CAGCTGAAAT GCGGAAGATG GTGAGACAGC CGAGCCGGGA GTCCACGGAT 
GGCAGCATCA ACAGTTATAG CTCGGAAGGA AACTTGATAT TTCCTGGAGT TCGAGTAGGA 
CCCGACAGTC AGTTCAGTGA TTTCCTTGAT GGGTTGGGAC CAGCGCAGCT CGTTGGCCGT 
CAGACGCTCG CCACCCCGGC CATGGGCGAT ATCCAAATCG GGATGGAGGA TAAGAAGGGT 
CAGTTGGAGG TTGAGGTTAT CAGAGCCCGG AGCCTTACAC AAAAACCTGG TTCCAAATCT 
ACACCCGCTC CCTATGTGAA AGTATATCTT TTGGAAAATG GAGCCTGTAT TGCCAAAAAG 
AAGACAAGAA TTGCACGGAA AACTCTCGAT CCTTTGTATC AGCAGTCCCT GGTTTTTGAT 
GAAAGTCCAC AGGGTAAAGT TCTTCAGGTG ATTGTCTGGG GTGACTATGG AAGAATGGAC 
CACAAATGCT TTATGGGTGT GGCTCAAATC TTGTTGGAAG AACTTGATCT ATCCAGCATG 
GTGATTGGAT GGTATAAATT GTTCCCTCCG TCCTCACTGG TGGATCCCAC TCTCGCTCCC 
CTGACCCGCC GGGCTTCCCA ATCATCTCTG GAAAGTTCGT CCGGGCCTCC CTGCATCCGG 
TCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017452161.1
CDS	315..4460
Translation	MSSAVGPRGPRPPTVPPPMQELPDLSHLTEEERNIIMAVMDRQKEEEEKEEAMLKCVVRDMAKPAACKTPRNAESQPHQPPLNIFRCVCVPRKPSSEEGGPERDWRLHQQFESYKEQVRKIGEEARRYQGEHKDDAPTCGICHKTKFADGCGHLCSYCRTKFCARCGGRVSLRSNNEDKVVMWVCNLCRKQQEILTKSGAWFFGSGPQQPSQDGTLSDTATGAGSEVPREKKARLQERSRSQTPLSTAAVSSQDTATPGAPLDRNKGAEPSQQALGPEQKQASRSRSEPPRERKKAPGLSEQNGKGGQKSERKRVPKSVVQPGEGIADERERKERRETRRLEKGRSQDYSDRPEKRDNGRVAEDQKQRKEEEYQTRYRSDPNLARYPVKAPPEEQQMRMHARVSRARHERRHSDVALPHTEAAAAAPAEATAGKRAPATARVSPPESPRARAPAAQPPTEHGPPPPRPVPGPAEPPEPRVPEPLRKQGRLDPASAVLLRKAKREKAESMLRNDSLSSDQSESVRPSPPKPHRPKRGGKRRQMSVSSSEEEGVSTPEYTSCEDVELESESVSEKGDLDYYWLDPATWHSRETSPISSHPVTWQPSKEGDRLIGRVILNKRTTMPKESGALLGLKVVGGKMTDLGRLGAFITKVKKGSLADVVGHLRAGDEVLEWNGKPLPGATNEEVYNIILESKSEPQVEIIVSRPIGDIPRIPESSHPPLESSSSSFESQKMERPSISVISPTSPGALKDAPQVLPGQLSVKLWYDKVGHQLIVNVLQATDLPPRVDGRPRNPYVKMYFLPDRSDKSKRRTKTVKKLLEPKWNQTFVYSHVHRRDFRERMLEITVWDQPRVQDEESEFLGEILIELETALLDDEPHWYKLQTHDESSLPLPQPSPFMPRRHIHGESSSKKLQRSQRISDSDISDYEVDDGIGVVPPVGYRASARESKATTLTVPEQQRTTHHRSRSVSPHRGDDQGRPRSRLPNVPLQRSLDEIHPTRRSRSPTRHHDASRSPADHRSRHVESQYSSEPDSELLMLPRAKRGRSAESLHMTSELQPSLDRARSASTNCLRPDTSLHSPERERHSRKSERCSIQKQSRKGTASDADRTHRQGSPTQSPPADTSFGSRRGRQLPQVPVRSGSIEQESGHKKLKSTIQRSTETGMAAEMRKMVRQPSRESTDGSINSYSSEGNLIFPGVRVGPDSQFSDFLDGLGPAQLVGRQTLATPAMGDIQIGMEDKKGQLEVEVIRARSLTQKPGSKSTPAPYVKVYLLENGACIAKKKTRIARKTLDPLYQQSLVFDESPQGKVLQVIVWGDYGRMDHKCFMGVAQILLEELDLSSMVIGWYKLFPPSSLVDPTLAPLTRRASQSSLESSSGPPCIRS

Target ORF information:

RefSeq Version	XM_017596672.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus regulating synaptic membrane exocytosis 1 (Rims1), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017596672.1

ORF Insert Sequence:

ATGTCCTCGG CCGTGGGGCC CCGAGGTCCT CGCCCACCCA CGGTGCCTCC CCCTATGCAA 
GAACTGCCCG ACCTGAGCCA CCTGACCGAG GAGGAGAGGA ACATTATCAT GGCAGTGATG 
GACCGGCAGA AGGAAGAGGA GGAAAAAGAA GAGGCCATGC TCAAGTGTGT TGTCAGGGAC 
ATGGCGAAGC CTGCTGCCTG CAAAACACCA AGAAATGCTG AAAGCCAGCC CCATCAACCA 
CCACTGAACA TTTTCAGATG TGTCTGTGTT CCCAGAAAGC CAAGCAGCGA AGAGGGAGGC 
CCAGAAAGAG ACTGGAGATT GCATCAACAG TTTGAAAGCT ACAAGGAGCA AGTGAGAAAA 
ATCGGAGAGG AAGCGAGGCG TTACCAGGGC GAGCACAAGG ATGATGCCCC GACGTGTGGA 
ATCTGTCATA AGACAAAGTT TGCTGATGGA TGTGGCCATC TCTGCTCCTA TTGTCGCACC 
AAGTTCTGTG CACGCTGCGG AGGCCGTGTG TCTCTGCGAT CGAACAATGA GGACAAAGTG 
GTTATGTGGG TATGCAATTT ATGTCGAAAG CAACAAGAAA TCTTAACGAA ATCTGGAGCG 
TGGTTCTTTG GAAGTGGCCC TCAGCAGCCT AGTCAAGATG GGACTCTGAG TGACACGGCC 
ACAGGTGCTG GATCTGAGGT GCCAAGAGAA AAGAAAGCAA GGCTCCAAGA GCGATCAAGG 
TCTCAGACGC CCTTGAGTAC AGCAGCTGTC TCTTCCCAAG ACACTGCTAC CCCCGGTGCA 
CCGTTGGACA GGAACAAAGG GGCTGAGCCC TCACAGCAAG CCTTGGGTCC TGAACAGAAG 
CAGGCATCAA GATCAAGAAG CGAGCCACCG AGGGAAAGGA AGAAGGCTCC AGGGCTTTCA 
GAGCAGAATG GCAAGGGAGG CCAGAAGAGC GAGCGCAAAC GTGTCCCCAA GTCTGTGGTG 
CAACCCGGGG AAGGGATCGC GGATGAGAGG GAGAGGAAAG AGAGGCGGGA AACCCGCAGG 
TTGGAGAAAG GGCGCTCCCA GGACTACTCA GACCGGCCTG AGAAACGCGA CAATGGCAGG 
GTGGCGGAAG ACCAGAAGCA GAGGAAGGAG GAGGAGTACC AGACTAGGTA CCGCAGCGAC 
CCTAACCTGG CTCGCTACCC GGTGAAGGCG CCGCCAGAGG AGCAGCAGAT GCGCATGCAC 
GCCCGGGTGT CCCGAGCGAG GCACGAGCGG CGCCACAGCG ACGTGGCGCT CCCGCACACC 
GAGGCAGCTG CCGCCGCGCC GGCTGAGGCC ACGGCGGGCA AGCGCGCGCC GGCCACCGCC 
AGGGTCTCTC CCCCGGAGTC CCCGCGCGCA CGCGCGCCCG CCGCCCAGCC TCCCACCGAG 
CACGGGCCAC CGCCGCCGCG GCCAGTCCCG GGTCCCGCAG AGCCACCCGA GCCGCGCGTC 
CCCGAGCCGC TCCGTAAGCA GGGCCGCCTG GACCCGGCCT CGGCCGTGCT TCTGCGCAAG 
GCCAAGCGCG AGAAGGCGGA GAGCATGCTG CGGAACGACT CGCTGAGCTC CGATCAGTCC 
GAGTCCGTGC GGCCATCCCC GCCCAAGCCT CACCGGCCCA AGCGGGGAGG CAAGAGACGT 
CAGATGTCGG TGAGCAGCTC GGAGGAGGAG GGCGTGTCCA CACCGGAGTA CACGAGCTGC 
GAGGACGTGG AGCTGGAGAG CGAGAGCGTG AGCGAGAAAG GTGACTTGGA TTACTACTGG 
TTGGATCCCG CCACGTGGCA CAGCAGGGAA ACGTCGCCTA TCAGTTCGCA TCCTGTAACG 
TGGCAGCCGT CTAAAGAGGG AGATCGACTA ATCGGCCGTG TTATTCTTAA CAAAAGAACA 
ACCATGCCCA AAGAATCAGG TGCATTATTG GGTCTGAAGG TGGTTGGAGG AAAAATGACG 
GACTTAGGGC GCCTTGGTGC TTTCATCACC AAAGTAAAGA AGGGCAGCCT GGCAGACGTC 
GTCGGACACC TAAGAGCAGG GGACGAAGTC CTAGAGTGGA ATGGTAAACC CCTGCCGGGA 
GCAACAAACG AAGAAGTTTA CAACATTATC TTAGAATCAA AATCAGAACC TCAAGTTGAG 
ATTATTGTTT CAAGGCCTAT TGGTGACATC CCCAGGATCC CTGAGAGTTC CCATCCTCCC 
CTGGAGTCCA GTTCAAGTTC CTTTGAATCT CAGAAAATGG AAAGGCCTTC TATTTCTGTT 
ATTTCTCCAA CCAGCCCTGG AGCTCTGAAA GATGCCCCAC AAGTCTTACC AGGGCAACTC 
TCAGTGAAGC TATGGTATGA TAAAGTGGGG CACCAGCTGA TTGTAAATGT TCTACAAGCA 
ACAGATCTAC CCCCTAGAGT AGATGGCCGT CCCAGGAATC CCTATGTAAA AATGTATTTT 
CTTCCAGATA GAAGCGACAA AAGTAAAAGG AGAACCAAAA CAGTAAAGAA ACTTCTAGAG 
CCAAAATGGA ACCAGACATT TGTCTACTCA CACGTACATC GTAGAGATTT TCGAGAGCGA 
ATGTTAGAGA TTACCGTGTG GGACCAGCCG AGAGTACAGG ACGAAGAGAG TGAATTTCTT 
GGAGAGATCC TCATAGAGTT GGAAACAGCG CTTTTAGATG ATGAGCCCCA TTGGTATAAA 
CTCCAGACAC ATGACGAATC TTCACTACCT CTGCCTCAGC CATCACCGTT CATGCCCAGG 
CGGCATATTC ATGGAGAGAG CTCCAGCAAA AAGCTACAAA GATCTCAGCG AATCAGTGAT 
AGTGACATCT CAGATTATGA GGTTGATGAT GGTATTGGAG TAGTGCCTCC AGTGGGTTAT 
AGAGCTAGTG CTAGAGAGAG TAAAGCAACC ACGTTAACAG TGCCAGAGCA ACAAAGAACT 
ACACATCACC GCTCACGTTC CGTGTCTCCT CATCGCGGCG ATGATCAGGG AAGGCCTCGT 
TCACGTTTAC CAAATGTGCC ATTACAGAGG AGCTTAGATG AAATTCATCC AACACGAAGG 
TCACGTTCTC CAACCCGACA CCATGATGCC TCCCGAAGCC CGGCCGATCA CAGATCCAGA 
CATGTGGAAA GTCAATATTC GTCAGAGCCA GACAGTGAGC TTCTCATGCT GCCCAGAGCA 
AAACGAGGAC GAAGTGCAGA AAGCCTACAC ATGACCAGTG AACTGCAGCC CTCTCTTGAC 
AGGGCTAGGA GTGCTAGTAC CAACTGCTTG AGACCAGATA CTAGTTTGCA TTCACCAGAA 
CGAGAAAGGC ACTCCAGAAA GTCTGAAAGA TGTAGCATCC AAAAACAGTC TAGGAAAGGC 
ACAGCCTCTG ATGCAGACAG AACGCACCGA CAAGGAAGCC CAACCCAGTC TCCTCCAGCA 
GACACATCCT TCGGCAGTCG CCGTGGAAGA CAGCTCCCAC AGGTGCCAGT TCGAAGCGGC 
AGTATAGAAC AAGAGTCGGG CCACAAGAAG TTGAAAAGCA CCATCCAGAG GAGTACGGAA 
ACAGGAATGG CAGCTGAAAT GCGGAAGATG GTGAGACAGC CGAGCCGGGA GTCCACGGAT 
GGCAGCATCA ACAGTTATAG CTCGGAAGGA AACTTGATAT TTCCTGGAGT TCGAGTAGGA 
CCCGACAGTC AGTTCAGTGA TTTCCTTGAT GGGTTGGGAC CAGCGCAGCT CGTTGGCCGT 
CAGACGCTCG CCACCCCGGC CATGGGCGAT ATCCAAATCG GGATGGAGGA TAAGAAGGGT 
CAGTTGGAGG TTGAGGTTAT CAGAGCCCGG AGCCTTACAC AAAAACCTGG TTCCAAATCT 
ACACCCGCTC CCTATGTGAA AGTATATCTT TTGGAAAATG GAGCCTGTAT TGCCAAAAAG 
AAGACAAGAA TTGCACGGAA AACTCTCGAT CCTTTGTATC AGCAGTCCCT GGTTTTTGAT 
GAAAGTCCAC AGGGTAAAGT TCTTCAGGTG ATTGTCTGGG GTGACTATGG AAGAATGGAC 
CACAAATGCT TTATGGGTGT GGCTCAAATC TTGTTGGAAG AACTTGATCT ATCCAGCATG 
GTGATTGGAT GGTATAAATT GTTCCCTCCG TCCTCACTGG TGGATCCCAC TCTCGCTCCC 
CTGACCCGCC GGGCTTCCCA ATCATCTCTG GAAAGTTCGT CCGGGCCTCC CTGCATCCGG 
TCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rims1 ( XM_017596673.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017452162.1 Rattus norvegicus regulating synaptic membrane exocytosis 1 (Rims1), transcript variant X20, mRNA.

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CloneID	ORa62890
Clone ID Related Accession (Same CDS sequence)	XM_017596673.1
Accession Version	XM_017596673.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4074bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	regulating synaptic membrane exocytosis protein 1 isoform X20
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005108.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCTCGG CCGTGGGGCC CCGAGGTCCT CGCCCACCCA CGGTGCCTCC CCCTATGCAA 
GAACTGCCCG ACCTGAGCCA CCTGACCGAG GAGGAGAGGA ACATTATCAT GGCAGTGATG 
GACCGGCAGA AGGAAGAGGA GGAAAAAGAA GAGGCCATGC TCAAGTGTGT TGTCAGGGAC 
ATGGCGAAGC CTGCTGCCTG CAAAACACCA AGAAATGCTG AAAGCCAGCC CCATCAACCA 
CCACTGAACA TTTTCAGATG TGTCTGTGTT CCCAGAAAGC CAAGCAGCGA AGAGGGAGGC 
CCAGAAAGAG ACTGGAGATT GCATCAACAG TTTGAAAGCT ACAAGGAGCA AGTGAGAAAA 
ATCGGAGAGG AAGCGAGGCG TTACCAGGGC GAGCACAAGG ATGATGCCCC GACGTGTGGA 
ATCTGTCATA AGACAAAGTT TGCTGATGGA TGTGGCCATC TCTGCTCCTA TTGTCGCACC 
AAGTTCTGTG CACGCTGCGG AGGCCGTGTG TCTCTGCGAT CGAACAATGA GGACAAAGTG 
GTTATGTGGG TATGCAATTT ATGTCGAAAG CAACAAGAAA TCTTAACGAA ATCTGGAGCG 
TGGTTCTTTG GAAGTGGCCC TCAGCAGCCT AGTCAAGATG GGACTCTGAG TGACACGGCC 
ACAGGTGCTG GATCTGAGGT GCCAAGAGAA AAGAAAGCAA GGCTCCAAGA GCGATCAAGG 
TCTCAGACGC CCTTGAGTAC AGCAGCTGTC TCTTCCCAAG ACACTGCTAC CCCCGGTGCA 
CCGTTGGACA GGAACAAAGG GGCTGAGCCC TCACAGCAAG CCTTGGGTCC TGAACAGAAG 
CAGGCATCAA GATCAAGAAG CGAGCCACCG AGGGAAAGGA AGAAGGCTCC AGGGCTTTCA 
GAGCAGAATG GCAAGGGAGG CCAGAAGAGC GAGCGCAAAC GTGTCCCCAA GTCTGTGGTG 
CAACCCGGGG AAGGGATCGC GGATGAGAGG GAGAGGAAAG AGAGGCGGGA AACCCGCAGG 
TTGGAGAAAG GGCGCTCCCA GGACTACTCA GACCGGCCTG AGAAACGCGA CAATGGCAGG 
GTGGCGGAAG ACCAGAAGCA GAGGAAGGAG GAGGAGTACC AGACTAGGTA CCGCAGCGAC 
CCTAACCTGG CTCGCTACCC GGTGAAGGCG CCGCCAGAGG AGCAGCAGAT GCGCATGCAC 
GCCCGGGTGT CCCGAGCGAG GCACGAGCGG CGCCACAGCG ACGTGGCGCT CCCGCACACC 
GAGGCAGCTG CCGCCGCGCC GGCTGAGGCC ACGGCGGGCA AGCGCGCGCC GGCCACCGCC 
AGGGTCTCTC CCCCGGAGTC CCCGCGCGCA CGCGCGCCCG CCGCCCAGCC TCCCACCGAG 
CACGGGCCAC CGCCGCCGCG GCCAGTCCCG GGTCCCGCAG AGCCACCCGA GCCGCGCGTC 
CCCGAGCCGC TCCGTAAGCA GGGCCGCCTG GACCCGGCCT CGGCCGTGCT TCTGCGCAAG 
GCCAAGCGCG AGAAGGCGGA GAGCATGCTG CGGAACGACT CGCTGAGCTC CGATCAGTCC 
GAGTCCGTGC GGCCATCCCC GCCCAAGCCT CACCGGCCCA AGCGGGGAGG CAAGAGACGT 
CAGATGTCGG TGAGCAGCTC GGAGGAGGAG GGCGTGTCCA CACCGGAGTA CACGAGCTGC 
GAGGACGTGG AGCTGGAGAG CGAGAGCGTG AGCGAGAAAG GTGACTTGGA TTACTACTGG 
TTGGATCCCG CCACGTGGCA CAGCAGGGAA ACGTCGCCTA TCAGTTCGCA TCCTGTAACG 
TGGCAGCCGT CTAAAGAGGG AGATCGACTA ATCGGCCGTG TTATTCTTAA CAAAAGAACA 
ACCATGCCCA AAGAATCAGG TGCATTATTG GGTCTGAAGG TGGTTGGAGG AAAAATGACG 
GACTTAGGGC GCCTTGGTGC TTTCATCACC AAAGTAAAGA AGGGCAGCCT GGCAGACGTC 
GTCGGACACC TAAGAGCAGG GGACGAAGTC CTAGAGTGGA ATGGTAAACC CCTGCCGGGA 
GCAACAAACG AAGAAGTTTA CAACATTATC TTAGAATCAA AATCAGAACC TCAAGTTGAG 
ATTATTGTTT CAAGGCCTAT TGGTGACATC CCCAGGATCC CTGAGAGTTC CCATCCTCCC 
CTGGAGTCCA GTTCAAGTTC CTTTGAATCT CAGAAAATGG AAAGGCCTTC TATTTCTGTT 
ATTTCTCCAA CCAGCCCTGG AGCTCTGAAA GATGCCCCAC AAGTCTTACC AGGGCAACTC 
TCAGTGAAGC TATGGTATGA TAAAGTGGGG CACCAGCTGA TTGTAAATGT TCTACAAGCA 
ACAGATCTAC CCCCTAGAGT AGATGGCCGT CCCAGGAATC CCTATGTAAA AATGTATTTT 
CTTCCAGATA GAAGCGACAA AAGTAAAAGG AGAACCAAAA CAGTAAAGAA ACTTCTAGAG 
CCAAAATGGA ACCAGACATT TGTCTACTCA CACGTACATC GTAGAGATTT TCGAGAGCGA 
ATGTTAGAGA TTACCGTGTG GGACCAGCCG AGAGTACAGG ACGAAGAGAG TGAATTTCTT 
GGAGAGATCC TCATAGAGTT GGAAACAGCG CTTTTAGATG ATGAGCCCCA TTGGTATAAA 
CTCCAGACAC ATGACGAATC TTCACTACCT CTGCCTCAGC CATCACCGTT CATGCCCAGG 
CGGCATATTC ATGGAGAGAG CTCCAGCAAA AAGCTACAAA GATCTCAGCG AATCAGTGAT 
AGTGACATCT CAGATTATGA GGTTGATGAT GGTATTGGAG TAGTGCCTCC AGTGGGTTAT 
AGAGCTAGTG CTAGAGAGAG TAAAGCAACC ACGTTAACAG TGCCAGAGCA ACAAAGAACT 
ACACATCACC GCTCACGTTC CGTGTCTCCT CATCGCGGCG ATGATCAGGG AAGGCCTCGT 
TCACGTTTAC CAAATGTGCC ATTACAGAGG AGCTTAGATG AAATTCATCC AACACGAAGG 
TCACGTTCTC CAACCCGACA CCATGATGCC TCCCGAAGCC CGGCCGATCA CAGATCCAGA 
CATGTGGAAA GTCAATATTC GTCAGAGCCA GACAGTGAGC TTCTCATGCT GCCCAGAGCA 
AAACGAGGAC GAAGTGCAGA AAGCCTACAC ATGACCAGTG AACTGCAGCC CTCTCTTGAC 
AGGGCTAGGA GTGCTAGTAC CAACTGCTTG AGACCAGATA CTAGTTTGCA TTCACCAGAA 
CGAGAAAGAA CGCACCGACA AGGAAGCCCA ACCCAGTCTC CTCCAGCAGA CACATCCTTC 
GGCAGTCGCC GTGGAAGACA GCTCCCACAG GTGCCAGTTC GAAGCGGCAG TATAGAACAA 
GAGTCGGGCC ACAAGAAGTT GAAAAGCACC ATCCAGAGGA GTACGGAAAC AGGAATGGCA 
GCTGAAATGC GGAAGATGGT GAGACAGCCG AGCCGGGAGT CCACGGATGG CAGCATCAAC 
AGTTATAGCT CGGAAGGAAA CTTGATATTT CCTGGAGTTC GAGTAGGACC CGACAGTCAG 
TTCAGTGATT TCCTTGATGG GTTGGGACCA GCGCAGCTCG TTGGCCGTCA GACGCTCGCC 
ACCCCGGCCA TGGGCGATAT CCAAATCGGG ATGGAGGATA AGAAGGGTCA GTTGGAGGTT 
GAGGTTATCA GAGCCCGGAG CCTTACACAA AAACCTGGTT CCAAATCTAC ACCCGCTCCC 
TATGTGAAAG TATATCTTTT GGAAAATGGA GCCTGTATTG CCAAAAAGAA GACAAGAATT 
GCACGGAAAA CTCTCGATCC TTTGTATCAG CAGTCCCTGG TTTTTGATGA AAGTCCACAG 
GGTAAAGTTC TTCAGGTGAT TGTCTGGGGT GACTATGGAA GAATGGACCA CAAATGCTTT 
ATGGGTGTGG CTCAAATCTT GTTGGAAGAA CTTGATCTAT CCAGCATGGT GATTGGATGG 
TATAAATTGT TCCCTCCGTC CTCACTGGTG GATCCCACTC TCGCTCCCCT GACCCGCCGG 
GCTTCCCAAT CATCTCTGGA AAGTTCGTCC GGGCCTCCCT GCATCCGGTC ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017452162.1
CDS	314..4387
Translation	MSSAVGPRGPRPPTVPPPMQELPDLSHLTEEERNIIMAVMDRQKEEEEKEEAMLKCVVRDMAKPAACKTPRNAESQPHQPPLNIFRCVCVPRKPSSEEGGPERDWRLHQQFESYKEQVRKIGEEARRYQGEHKDDAPTCGICHKTKFADGCGHLCSYCRTKFCARCGGRVSLRSNNEDKVVMWVCNLCRKQQEILTKSGAWFFGSGPQQPSQDGTLSDTATGAGSEVPREKKARLQERSRSQTPLSTAAVSSQDTATPGAPLDRNKGAEPSQQALGPEQKQASRSRSEPPRERKKAPGLSEQNGKGGQKSERKRVPKSVVQPGEGIADERERKERRETRRLEKGRSQDYSDRPEKRDNGRVAEDQKQRKEEEYQTRYRSDPNLARYPVKAPPEEQQMRMHARVSRARHERRHSDVALPHTEAAAAAPAEATAGKRAPATARVSPPESPRARAPAAQPPTEHGPPPPRPVPGPAEPPEPRVPEPLRKQGRLDPASAVLLRKAKREKAESMLRNDSLSSDQSESVRPSPPKPHRPKRGGKRRQMSVSSSEEEGVSTPEYTSCEDVELESESVSEKGDLDYYWLDPATWHSRETSPISSHPVTWQPSKEGDRLIGRVILNKRTTMPKESGALLGLKVVGGKMTDLGRLGAFITKVKKGSLADVVGHLRAGDEVLEWNGKPLPGATNEEVYNIILESKSEPQVEIIVSRPIGDIPRIPESSHPPLESSSSSFESQKMERPSISVISPTSPGALKDAPQVLPGQLSVKLWYDKVGHQLIVNVLQATDLPPRVDGRPRNPYVKMYFLPDRSDKSKRRTKTVKKLLEPKWNQTFVYSHVHRRDFRERMLEITVWDQPRVQDEESEFLGEILIELETALLDDEPHWYKLQTHDESSLPLPQPSPFMPRRHIHGESSSKKLQRSQRISDSDISDYEVDDGIGVVPPVGYRASARESKATTLTVPEQQRTTHHRSRSVSPHRGDDQGRPRSRLPNVPLQRSLDEIHPTRRSRSPTRHHDASRSPADHRSRHVESQYSSEPDSELLMLPRAKRGRSAESLHMTSELQPSLDRARSASTNCLRPDTSLHSPERERTHRQGSPTQSPPADTSFGSRRGRQLPQVPVRSGSIEQESGHKKLKSTIQRSTETGMAAEMRKMVRQPSRESTDGSINSYSSEGNLIFPGVRVGPDSQFSDFLDGLGPAQLVGRQTLATPAMGDIQIGMEDKKGQLEVEVIRARSLTQKPGSKSTPAPYVKVYLLENGACIAKKKTRIARKTLDPLYQQSLVFDESPQGKVLQVIVWGDYGRMDHKCFMGVAQILLEELDLSSMVIGWYKLFPPSSLVDPTLAPLTRRASQSSLESSSGPPCIRS

Target ORF information:

RefSeq Version	XM_017596673.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus regulating synaptic membrane exocytosis 1 (Rims1), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017596673.1

ORF Insert Sequence:

ATGTCCTCGG CCGTGGGGCC CCGAGGTCCT CGCCCACCCA CGGTGCCTCC CCCTATGCAA 
GAACTGCCCG ACCTGAGCCA CCTGACCGAG GAGGAGAGGA ACATTATCAT GGCAGTGATG 
GACCGGCAGA AGGAAGAGGA GGAAAAAGAA GAGGCCATGC TCAAGTGTGT TGTCAGGGAC 
ATGGCGAAGC CTGCTGCCTG CAAAACACCA AGAAATGCTG AAAGCCAGCC CCATCAACCA 
CCACTGAACA TTTTCAGATG TGTCTGTGTT CCCAGAAAGC CAAGCAGCGA AGAGGGAGGC 
CCAGAAAGAG ACTGGAGATT GCATCAACAG TTTGAAAGCT ACAAGGAGCA AGTGAGAAAA 
ATCGGAGAGG AAGCGAGGCG TTACCAGGGC GAGCACAAGG ATGATGCCCC GACGTGTGGA 
ATCTGTCATA AGACAAAGTT TGCTGATGGA TGTGGCCATC TCTGCTCCTA TTGTCGCACC 
AAGTTCTGTG CACGCTGCGG AGGCCGTGTG TCTCTGCGAT CGAACAATGA GGACAAAGTG 
GTTATGTGGG TATGCAATTT ATGTCGAAAG CAACAAGAAA TCTTAACGAA ATCTGGAGCG 
TGGTTCTTTG GAAGTGGCCC TCAGCAGCCT AGTCAAGATG GGACTCTGAG TGACACGGCC 
ACAGGTGCTG GATCTGAGGT GCCAAGAGAA AAGAAAGCAA GGCTCCAAGA GCGATCAAGG 
TCTCAGACGC CCTTGAGTAC AGCAGCTGTC TCTTCCCAAG ACACTGCTAC CCCCGGTGCA 
CCGTTGGACA GGAACAAAGG GGCTGAGCCC TCACAGCAAG CCTTGGGTCC TGAACAGAAG 
CAGGCATCAA GATCAAGAAG CGAGCCACCG AGGGAAAGGA AGAAGGCTCC AGGGCTTTCA 
GAGCAGAATG GCAAGGGAGG CCAGAAGAGC GAGCGCAAAC GTGTCCCCAA GTCTGTGGTG 
CAACCCGGGG AAGGGATCGC GGATGAGAGG GAGAGGAAAG AGAGGCGGGA AACCCGCAGG 
TTGGAGAAAG GGCGCTCCCA GGACTACTCA GACCGGCCTG AGAAACGCGA CAATGGCAGG 
GTGGCGGAAG ACCAGAAGCA GAGGAAGGAG GAGGAGTACC AGACTAGGTA CCGCAGCGAC 
CCTAACCTGG CTCGCTACCC GGTGAAGGCG CCGCCAGAGG AGCAGCAGAT GCGCATGCAC 
GCCCGGGTGT CCCGAGCGAG GCACGAGCGG CGCCACAGCG ACGTGGCGCT CCCGCACACC 
GAGGCAGCTG CCGCCGCGCC GGCTGAGGCC ACGGCGGGCA AGCGCGCGCC GGCCACCGCC 
AGGGTCTCTC CCCCGGAGTC CCCGCGCGCA CGCGCGCCCG CCGCCCAGCC TCCCACCGAG 
CACGGGCCAC CGCCGCCGCG GCCAGTCCCG GGTCCCGCAG AGCCACCCGA GCCGCGCGTC 
CCCGAGCCGC TCCGTAAGCA GGGCCGCCTG GACCCGGCCT CGGCCGTGCT TCTGCGCAAG 
GCCAAGCGCG AGAAGGCGGA GAGCATGCTG CGGAACGACT CGCTGAGCTC CGATCAGTCC 
GAGTCCGTGC GGCCATCCCC GCCCAAGCCT CACCGGCCCA AGCGGGGAGG CAAGAGACGT 
CAGATGTCGG TGAGCAGCTC GGAGGAGGAG GGCGTGTCCA CACCGGAGTA CACGAGCTGC 
GAGGACGTGG AGCTGGAGAG CGAGAGCGTG AGCGAGAAAG GTGACTTGGA TTACTACTGG 
TTGGATCCCG CCACGTGGCA CAGCAGGGAA ACGTCGCCTA TCAGTTCGCA TCCTGTAACG 
TGGCAGCCGT CTAAAGAGGG AGATCGACTA ATCGGCCGTG TTATTCTTAA CAAAAGAACA 
ACCATGCCCA AAGAATCAGG TGCATTATTG GGTCTGAAGG TGGTTGGAGG AAAAATGACG 
GACTTAGGGC GCCTTGGTGC TTTCATCACC AAAGTAAAGA AGGGCAGCCT GGCAGACGTC 
GTCGGACACC TAAGAGCAGG GGACGAAGTC CTAGAGTGGA ATGGTAAACC CCTGCCGGGA 
GCAACAAACG AAGAAGTTTA CAACATTATC TTAGAATCAA AATCAGAACC TCAAGTTGAG 
ATTATTGTTT CAAGGCCTAT TGGTGACATC CCCAGGATCC CTGAGAGTTC CCATCCTCCC 
CTGGAGTCCA GTTCAAGTTC CTTTGAATCT CAGAAAATGG AAAGGCCTTC TATTTCTGTT 
ATTTCTCCAA CCAGCCCTGG AGCTCTGAAA GATGCCCCAC AAGTCTTACC AGGGCAACTC 
TCAGTGAAGC TATGGTATGA TAAAGTGGGG CACCAGCTGA TTGTAAATGT TCTACAAGCA 
ACAGATCTAC CCCCTAGAGT AGATGGCCGT CCCAGGAATC CCTATGTAAA AATGTATTTT 
CTTCCAGATA GAAGCGACAA AAGTAAAAGG AGAACCAAAA CAGTAAAGAA ACTTCTAGAG 
CCAAAATGGA ACCAGACATT TGTCTACTCA CACGTACATC GTAGAGATTT TCGAGAGCGA 
ATGTTAGAGA TTACCGTGTG GGACCAGCCG AGAGTACAGG ACGAAGAGAG TGAATTTCTT 
GGAGAGATCC TCATAGAGTT GGAAACAGCG CTTTTAGATG ATGAGCCCCA TTGGTATAAA 
CTCCAGACAC ATGACGAATC TTCACTACCT CTGCCTCAGC CATCACCGTT CATGCCCAGG 
CGGCATATTC ATGGAGAGAG CTCCAGCAAA AAGCTACAAA GATCTCAGCG AATCAGTGAT 
AGTGACATCT CAGATTATGA GGTTGATGAT GGTATTGGAG TAGTGCCTCC AGTGGGTTAT 
AGAGCTAGTG CTAGAGAGAG TAAAGCAACC ACGTTAACAG TGCCAGAGCA ACAAAGAACT 
ACACATCACC GCTCACGTTC CGTGTCTCCT CATCGCGGCG ATGATCAGGG AAGGCCTCGT 
TCACGTTTAC CAAATGTGCC ATTACAGAGG AGCTTAGATG AAATTCATCC AACACGAAGG 
TCACGTTCTC CAACCCGACA CCATGATGCC TCCCGAAGCC CGGCCGATCA CAGATCCAGA 
CATGTGGAAA GTCAATATTC GTCAGAGCCA GACAGTGAGC TTCTCATGCT GCCCAGAGCA 
AAACGAGGAC GAAGTGCAGA AAGCCTACAC ATGACCAGTG AACTGCAGCC CTCTCTTGAC 
AGGGCTAGGA GTGCTAGTAC CAACTGCTTG AGACCAGATA CTAGTTTGCA TTCACCAGAA 
CGAGAAAGAA CGCACCGACA AGGAAGCCCA ACCCAGTCTC CTCCAGCAGA CACATCCTTC 
GGCAGTCGCC GTGGAAGACA GCTCCCACAG GTGCCAGTTC GAAGCGGCAG TATAGAACAA 
GAGTCGGGCC ACAAGAAGTT GAAAAGCACC ATCCAGAGGA GTACGGAAAC AGGAATGGCA 
GCTGAAATGC GGAAGATGGT GAGACAGCCG AGCCGGGAGT CCACGGATGG CAGCATCAAC 
AGTTATAGCT CGGAAGGAAA CTTGATATTT CCTGGAGTTC GAGTAGGACC CGACAGTCAG 
TTCAGTGATT TCCTTGATGG GTTGGGACCA GCGCAGCTCG TTGGCCGTCA GACGCTCGCC 
ACCCCGGCCA TGGGCGATAT CCAAATCGGG ATGGAGGATA AGAAGGGTCA GTTGGAGGTT 
GAGGTTATCA GAGCCCGGAG CCTTACACAA AAACCTGGTT CCAAATCTAC ACCCGCTCCC 
TATGTGAAAG TATATCTTTT GGAAAATGGA GCCTGTATTG CCAAAAAGAA GACAAGAATT 
GCACGGAAAA CTCTCGATCC TTTGTATCAG CAGTCCCTGG TTTTTGATGA AAGTCCACAG 
GGTAAAGTTC TTCAGGTGAT TGTCTGGGGT GACTATGGAA GAATGGACCA CAAATGCTTT 
ATGGGTGTGG CTCAAATCTT GTTGGAAGAA CTTGATCTAT CCAGCATGGT GATTGGATGG 
TATAAATTGT TCCCTCCGTC CTCACTGGTG GATCCCACTC TCGCTCCCCT GACCCGCCGG 
GCTTCCCAAT CATCTCTGGA AAGTTCGTCC GGGCCTCCCT GCATCCGGTC ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rims1 ( XM_017596678.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017452167.1 Rattus norvegicus regulating synaptic membrane exocytosis 1 (Rims1), transcript variant X25, mRNA.

Price & Availability↑

CloneID	ORa62895
Clone ID Related Accession (Same CDS sequence)	XM_017596678.1
Accession Version	XM_017596678.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4779bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	regulating synaptic membrane exocytosis protein 1 isoform X25
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005108.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCTCGG CCGTGGGGCC CCGAGGTCCT CGCCCACCCA CGGTGCCTCC CCCTATGCAA 
GAACTGCCCG ACCTGAGCCA CCTGACCGAG GAGGAGAGGA ACATTATCAT GGCAGTGATG 
GACCGGCAGA AGGAAGAGGA GGAAAAAGAA GAGGCCATGC TCAAGAGATT GCATCAACAG 
TTTGAAAGCT ACAAGGAGCA AGTGAGAAAA ATCGGAGAGG AAGCGAGGCG TTACCAGGGC 
GAGCACAAGG ATGATGCCCC GACGTGTGGA ATCTGTCATA AGACAAAGTT TGCTGATGGA 
TGTGGCCATC TCTGCTCCTA TTGTCGCACC AAGTTCTGTG CACGCTGCGG AGGCCGTGTG 
TCTCTGCGAT CGAACAATGA GGACAAAGTG GTTATGTGGG TATGCAATTT ATGTCGAAAG 
CAACAAGAAA TCTTAACGAA ATCTGGAGCG TGGTTCTTTG GAAGTGGCCC TCAGCAGCCT 
AGTCAAGATG GGACTCTGAG TGACACGGCC ACAGGTGCTG GATCTGAGGT GCCAAGAGAA 
AAGAAAGCAA GGCTCCAAGA GCGATCAAGG TCTCAGACGC CCTTGAGTAC AGCAGCTGTC 
TCTTCCCAAG ACACTGCTAC CCCCGGTGCA CCGTTGGACA GGAACAAAGG GGCTGAGCCC 
TCACAGCAAG CCTTGGGTCC TGAACAGAAG CAGGCATCAA GATCAAGAAG CGAGCCACCG 
AGGGAAAGGA AGAAGGCTCC AGGGCTTTCA GAGCAGAATG GCAAGGGAGG CCAGAAGAGC 
GAGCGCAAAC GTGTCCCCAA GTCTGTGGTG CAACCCGGGG AAGGGATCGC GGATGAGAGG 
GAGAGGAAAG AGAGGCGGGA AACCCGCAGG TTGGAGAAAG GGCGCTCCCA GGACTACTCA 
GACCGGCCTG AGAAACGCGA CAATGGCAGG GTGGCGGAAG ACCAGAAGCA GAGGAAGGAG 
GAGGAGTACC AGACTAGGTA CCGCAGCGAC CCTAACCTGG CTCGCTACCC GGTGAAGGCG 
CCGCCAGAGG AGCAGCAGAT GCGCATGCAC GCCCGGGTGT CCCGAGCGAG GCACGAGCGG 
CGCCACAGCG ACGTGGCGCT CCCGCACACC GAGGCAGCTG CCGCCGCGCC GGCTGAGGCC 
ACGGCGGGCA AGCGCGCGCC GGCCACCGCC AGGGTCTCTC CCCCGGAGTC CCCGCGCGCA 
CGCGCGCCCG CCGCCCAGCC TCCCACCGAG CACGGGCCAC CGCCGCCGCG GCCAGTCCCG 
GGTCCCGCAG AGCCACCCGA GCCGCGCGTC CCCGAGCCGC TCCGTAAGCA GGGCCGCCTG 
GACCCGGCCT CGGCCGTGCT TCTGCGCAAG GCCAAGCGCG AGAAGGCGGA GAGCATGCTG 
CGGAACGACT CGCTGAGCTC CGATCAGTCC GAGTCCGTGC GGCCATCCCC GCCCAAGCCT 
CACCGGCCCA AGCGGGGAGG CAAGAGACGT CAGATGTCGG TGAGCAGCTC GGAGGAGGAG 
GGCGTGTCCA CACCGGAGTA CACGAGCTGC GAGGACGTGG AGCTGGAGAG CGAGAGCGTG 
AGCGAGAAAG GTGACTTGGA TTACTACTGG TTGGATCCCG CCACGTGGCA CAGCAGGGAA 
ACGTCGCCTA TCAGTTCGCA TCCTGTAACG TGGCAGCCGT CTAAAGAGGG AGATCGACTA 
ATCGGCCGTG TTATTCTTAA CAAAAGAACA ACCATGCCCA AAGAATCAGG TGCATTATTG 
GGTCTGAAGG TGGTTGGAGG AAAAATGACG GACTTAGGGC GCCTTGGTGC TTTCATCACC 
AAAGTAAAGA AGGGCAGCCT GGCAGACGTC GTCGGACACC TAAGAGCAGG GGACGAAGTC 
CTAGAGTGGA ATGGTAAACC CCTGCCGGGA GCAACAAACG AAGAAGTTTA CAACATTATC 
TTAGAATCAA AATCAGAACC TCAAGTTGAG ATTATTGTTT CAAGGCCTAT TGGTGACATC 
CCCAGGATCC CTGAGAGTTC CCATCCTCCC CTGGAGTCCA GTTCAAGTTC CTTTGAATCT 
CAGAAAATGG AAAGGCCTTC TATTTCTGTT ATTTCTCCAA CCAGCCCTGG AGCTCTGAAA 
GATGCCCCAC AAGTCTTACC AGGGCAACTC TCAGTGAAGC TATGGTATGA TAAAGTGGGG 
CACCAGCTGA TTGTAAATGT TCTACAAGCA ACAGATCTAC CCCCTAGAGT AGATGGCCGT 
CCCAGGAATC CCTATGTAAA AATGTATTTT CTTCCAGATA GAAGCGACAA AAGTAAAAGG 
AGAACCAAAA CAGTAAAGAA ACTTCTAGAG CCAAAATGGA ACCAGACATT TGTCTACTCA 
CACGTACATC GTAGAGATTT TCGAGAGCGA ATGTTAGAGA TTACCGTGTG GGACCAGCCG 
AGAGTACAGG ACGAAGAGAG TGAATTTCTT GGAGAGATCC TCATAGAGTT GGAAACAGCG 
CTTTTAGATG ATGAGCCCCA TTGGTATAAA CTCCAGACAC ATGACGAATC TTCACTACCT 
CTGCCTCAGC CATCACCGTT CATGCCCAGG CGGCATATTC ATGGAGAGAG CTCCAGCAAA 
AAGCTACAAA GATCTCAGCG AATCAGTGAT AGTGACATCT CAGATTATGA GGTTGATGAT 
GGTATTGGAG TAGTGCCTCC AGTGGGTTAT AGAGCTAGTG CTAGAGAGAG TAAAGCAACC 
ACGTTAACAG TGCCAGAGCA ACAAAGAACT ACACATCACC GCTCACGTTC CGTGTCTCCT 
CATCGCGGCG ATGATCAGGG AAGGCCTCGT TCACGTTTAC CAAATGTGCC ATTACAGAGG 
AGCTTAGATG AAATTCATCC AACACGAAGG TCACGTTCTC CAACCCGACA CCATGATGCC 
TCCCGAAGCC CGGCCGATCA CAGATCCAGA CATGTGGAAA GTCAATATTC GTCAGAGCCA 
GACAGTGAGC TTCTCATGCT GCCCAGAGCA AAACGAGGAC GAAGTGCAGA AAGCCTACAC 
ATGACCAGTG AACTGCAGCC CTCTCTTGAC AGGGCTAGGA GTGCTAGTAC CAACTGCTTG 
AGACCAGATA CTAGTTTGCA TTCACCAGAA CGAGAAAGGG GTAGATGGTC CCCCTCCCTA 
GATAGGAGGC GACCTGCTAG CCCCAGGATT CAAATCCAGC ATGCATCTCC GGAGAATGAC 
AGGCACTCCA GAAAGTCTGA AAGATGTAGC ATCCAAAAAC AGTCTAGGAA AGGCACAGCC 
TCTGATGCAG ACAGGGTTCT CCCACCATGC CTTTCTAGAA GGGGATACGC AACCCCAAGA 
GCAACCGATC AACCGGTCGT TAGGGGAAAG CATCCCACTC GTTCACGGTC GAGCGAGCAC 
TCTAGTGTCA GAACCCTGTG TTCTATGCAC CACCTTGCCC CCGGAGGGTC GGCGCCACCT 
TCTCCACTTC TGACAAGAAC GCACCGACAA GGAAGCCCAA CCCAGTCTCC TCCAGCAGAC 
ACATCCTTCG GCAGTCGCCG TGGAAGACAG CTCCCACAGG TGCCAGTTCG AAGCGGCAGT 
ATAGAACAAG CAAGCTTAGT AGTGGAGGAG CGAACGAGAC AGATGAAAAT GAAAGTTCAC 
CGATTTAAGC AGACAACAGG GTCTGGGTCT AGTCAAGAAC TTGACCACGA GCAATACTCC 
AAGTACAACA TACATAAAGA TCAGTACAGA AGCTGTGATA ACGCGTCTGC CAAGTCTTCA 
GATAGTGATG TCAGTGATGT GTCCGCCATT TCCAGAGCCA GCAGTACCTC ACGCCTCAGC 
AGCACAAGCT TTATGTCAGA GCAGTCTGAG CGCCCCAGGG GTAGGATCAG TTCATTTACC 
CCCAAAATGC AAGGCAGACG GATGGGGACT TCAGGAAGAG CCATCATCAA GAGCACCAGT 
GTAAGTGGAG AGATATATAC ACTGGAACGT AATGACGGTA GCCAGTCGGA CACGGCCGTG 
GGTACCGTCG GAGCCGGTGG AAAGAAACGA AGATCCAGCC TGAGCGCCAA AGTGGTAGCC 
ATTGTGTCTC GAAGAAGCAG GAGCACGTCA CAGCTCAGCC AGACAGAGTC GGGCCACAAG 
AAGTTGAAAA GCACCATCCA GAGGAGTACG GAAACAGGAA TGGCAGCTGA AATGCGGAAG 
ATGGTGAGAC AGCCGAGCCG GGAGTCCACG GATGGCAGCA TCAACAGTTA TAGCTCGGAA 
GGAAACTTGA TATTTCCTGG AGTTCGAGTA GGACCCGACA GTCAGTTCAG TGATTTCCTT 
GATGGGTTGG GACCAGCGCA GCTCGTTGGC CGTCAGACGC TCGCCACCCC GGCCATGGGC 
GATATCCAAA TCGGGATGGA GGATAAGAAG GGTCAGTTGG AGGTTGAGGT TATCAGAGCC 
CGGAGCCTTA CACAAAAACC TGGTTCCAAA TCTACACCCG CTCCCTATGT GAAAGTATAT 
CTTTTGGAAA ATGGAGCCTG TATTGCCAAA AAGAAGACAA GAATTGCACG GAAAACTCTC 
GATCCTTTGT ATCAGCAGTC CCTGGTTTTT GATGAAAGTC CACAGGGTAA AGTTCTTCAG 
GTGATTGTCT GGGGTGACTA TGGAAGAATG GACCACAAAT GCTTTATGGG TGTGGCTCAA 
ATCTTGTTGG AAGAACTTGA TCTATCCAGC ATGGTGATTG GATGGTATAA ATTGTTCCCT 
CCGTCCTCAC TGGTGGATCC CACTCTCGCT CCCCTGACCC GCCGGGCTTC CCAATCATCT 
CTGGAAAGTT CGTCCGGGCC TCCCTGCATC CGGTCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017452167.1
CDS	311..5089
Translation	MSSAVGPRGPRPPTVPPPMQELPDLSHLTEEERNIIMAVMDRQKEEEEKEEAMLKRLHQQFESYKEQVRKIGEEARRYQGEHKDDAPTCGICHKTKFADGCGHLCSYCRTKFCARCGGRVSLRSNNEDKVVMWVCNLCRKQQEILTKSGAWFFGSGPQQPSQDGTLSDTATGAGSEVPREKKARLQERSRSQTPLSTAAVSSQDTATPGAPLDRNKGAEPSQQALGPEQKQASRSRSEPPRERKKAPGLSEQNGKGGQKSERKRVPKSVVQPGEGIADERERKERRETRRLEKGRSQDYSDRPEKRDNGRVAEDQKQRKEEEYQTRYRSDPNLARYPVKAPPEEQQMRMHARVSRARHERRHSDVALPHTEAAAAAPAEATAGKRAPATARVSPPESPRARAPAAQPPTEHGPPPPRPVPGPAEPPEPRVPEPLRKQGRLDPASAVLLRKAKREKAESMLRNDSLSSDQSESVRPSPPKPHRPKRGGKRRQMSVSSSEEEGVSTPEYTSCEDVELESESVSEKGDLDYYWLDPATWHSRETSPISSHPVTWQPSKEGDRLIGRVILNKRTTMPKESGALLGLKVVGGKMTDLGRLGAFITKVKKGSLADVVGHLRAGDEVLEWNGKPLPGATNEEVYNIILESKSEPQVEIIVSRPIGDIPRIPESSHPPLESSSSSFESQKMERPSISVISPTSPGALKDAPQVLPGQLSVKLWYDKVGHQLIVNVLQATDLPPRVDGRPRNPYVKMYFLPDRSDKSKRRTKTVKKLLEPKWNQTFVYSHVHRRDFRERMLEITVWDQPRVQDEESEFLGEILIELETALLDDEPHWYKLQTHDESSLPLPQPSPFMPRRHIHGESSSKKLQRSQRISDSDISDYEVDDGIGVVPPVGYRASARESKATTLTVPEQQRTTHHRSRSVSPHRGDDQGRPRSRLPNVPLQRSLDEIHPTRRSRSPTRHHDASRSPADHRSRHVESQYSSEPDSELLMLPRAKRGRSAESLHMTSELQPSLDRARSASTNCLRPDTSLHSPERERGRWSPSLDRRRPASPRIQIQHASPENDRHSRKSERCSIQKQSRKGTASDADRVLPPCLSRRGYATPRATDQPVVRGKHPTRSRSSEHSSVRTLCSMHHLAPGGSAPPSPLLTRTHRQGSPTQSPPADTSFGSRRGRQLPQVPVRSGSIEQASLVVEERTRQMKMKVHRFKQTTGSGSSQELDHEQYSKYNIHKDQYRSCDNASAKSSDSDVSDVSAISRASSTSRLSSTSFMSEQSERPRGRISSFTPKMQGRRMGTSGRAIIKSTSVSGEIYTLERNDGSQSDTAVGTVGAGGKKRRSSLSAKVVAIVSRRSRSTSQLSQTESGHKKLKSTIQRSTETGMAAEMRKMVRQPSRESTDGSINSYSSEGNLIFPGVRVGPDSQFSDFLDGLGPAQLVGRQTLATPAMGDIQIGMEDKKGQLEVEVIRARSLTQKPGSKSTPAPYVKVYLLENGACIAKKKTRIARKTLDPLYQQSLVFDESPQGKVLQVIVWGDYGRMDHKCFMGVAQILLEELDLSSMVIGWYKLFPPSSLVDPTLAPLTRRASQSSLESSSGPPCIRS

Target ORF information:

RefSeq Version	XM_017596678.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus regulating synaptic membrane exocytosis 1 (Rims1), transcript variant X25, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017596678.1

ORF Insert Sequence:

ATGTCCTCGG CCGTGGGGCC CCGAGGTCCT CGCCCACCCA CGGTGCCTCC CCCTATGCAA 
GAACTGCCCG ACCTGAGCCA CCTGACCGAG GAGGAGAGGA ACATTATCAT GGCAGTGATG 
GACCGGCAGA AGGAAGAGGA GGAAAAAGAA GAGGCCATGC TCAAGAGATT GCATCAACAG 
TTTGAAAGCT ACAAGGAGCA AGTGAGAAAA ATCGGAGAGG AAGCGAGGCG TTACCAGGGC 
GAGCACAAGG ATGATGCCCC GACGTGTGGA ATCTGTCATA AGACAAAGTT TGCTGATGGA 
TGTGGCCATC TCTGCTCCTA TTGTCGCACC AAGTTCTGTG CACGCTGCGG AGGCCGTGTG 
TCTCTGCGAT CGAACAATGA GGACAAAGTG GTTATGTGGG TATGCAATTT ATGTCGAAAG 
CAACAAGAAA TCTTAACGAA ATCTGGAGCG TGGTTCTTTG GAAGTGGCCC TCAGCAGCCT 
AGTCAAGATG GGACTCTGAG TGACACGGCC ACAGGTGCTG GATCTGAGGT GCCAAGAGAA 
AAGAAAGCAA GGCTCCAAGA GCGATCAAGG TCTCAGACGC CCTTGAGTAC AGCAGCTGTC 
TCTTCCCAAG ACACTGCTAC CCCCGGTGCA CCGTTGGACA GGAACAAAGG GGCTGAGCCC 
TCACAGCAAG CCTTGGGTCC TGAACAGAAG CAGGCATCAA GATCAAGAAG CGAGCCACCG 
AGGGAAAGGA AGAAGGCTCC AGGGCTTTCA GAGCAGAATG GCAAGGGAGG CCAGAAGAGC 
GAGCGCAAAC GTGTCCCCAA GTCTGTGGTG CAACCCGGGG AAGGGATCGC GGATGAGAGG 
GAGAGGAAAG AGAGGCGGGA AACCCGCAGG TTGGAGAAAG GGCGCTCCCA GGACTACTCA 
GACCGGCCTG AGAAACGCGA CAATGGCAGG GTGGCGGAAG ACCAGAAGCA GAGGAAGGAG 
GAGGAGTACC AGACTAGGTA CCGCAGCGAC CCTAACCTGG CTCGCTACCC GGTGAAGGCG 
CCGCCAGAGG AGCAGCAGAT GCGCATGCAC GCCCGGGTGT CCCGAGCGAG GCACGAGCGG 
CGCCACAGCG ACGTGGCGCT CCCGCACACC GAGGCAGCTG CCGCCGCGCC GGCTGAGGCC 
ACGGCGGGCA AGCGCGCGCC GGCCACCGCC AGGGTCTCTC CCCCGGAGTC CCCGCGCGCA 
CGCGCGCCCG CCGCCCAGCC TCCCACCGAG CACGGGCCAC CGCCGCCGCG GCCAGTCCCG 
GGTCCCGCAG AGCCACCCGA GCCGCGCGTC CCCGAGCCGC TCCGTAAGCA GGGCCGCCTG 
GACCCGGCCT CGGCCGTGCT TCTGCGCAAG GCCAAGCGCG AGAAGGCGGA GAGCATGCTG 
CGGAACGACT CGCTGAGCTC CGATCAGTCC GAGTCCGTGC GGCCATCCCC GCCCAAGCCT 
CACCGGCCCA AGCGGGGAGG CAAGAGACGT CAGATGTCGG TGAGCAGCTC GGAGGAGGAG 
GGCGTGTCCA CACCGGAGTA CACGAGCTGC GAGGACGTGG AGCTGGAGAG CGAGAGCGTG 
AGCGAGAAAG GTGACTTGGA TTACTACTGG TTGGATCCCG CCACGTGGCA CAGCAGGGAA 
ACGTCGCCTA TCAGTTCGCA TCCTGTAACG TGGCAGCCGT CTAAAGAGGG AGATCGACTA 
ATCGGCCGTG TTATTCTTAA CAAAAGAACA ACCATGCCCA AAGAATCAGG TGCATTATTG 
GGTCTGAAGG TGGTTGGAGG AAAAATGACG GACTTAGGGC GCCTTGGTGC TTTCATCACC 
AAAGTAAAGA AGGGCAGCCT GGCAGACGTC GTCGGACACC TAAGAGCAGG GGACGAAGTC 
CTAGAGTGGA ATGGTAAACC CCTGCCGGGA GCAACAAACG AAGAAGTTTA CAACATTATC 
TTAGAATCAA AATCAGAACC TCAAGTTGAG ATTATTGTTT CAAGGCCTAT TGGTGACATC 
CCCAGGATCC CTGAGAGTTC CCATCCTCCC CTGGAGTCCA GTTCAAGTTC CTTTGAATCT 
CAGAAAATGG AAAGGCCTTC TATTTCTGTT ATTTCTCCAA CCAGCCCTGG AGCTCTGAAA 
GATGCCCCAC AAGTCTTACC AGGGCAACTC TCAGTGAAGC TATGGTATGA TAAAGTGGGG 
CACCAGCTGA TTGTAAATGT TCTACAAGCA ACAGATCTAC CCCCTAGAGT AGATGGCCGT 
CCCAGGAATC CCTATGTAAA AATGTATTTT CTTCCAGATA GAAGCGACAA AAGTAAAAGG 
AGAACCAAAA CAGTAAAGAA ACTTCTAGAG CCAAAATGGA ACCAGACATT TGTCTACTCA 
CACGTACATC GTAGAGATTT TCGAGAGCGA ATGTTAGAGA TTACCGTGTG GGACCAGCCG 
AGAGTACAGG ACGAAGAGAG TGAATTTCTT GGAGAGATCC TCATAGAGTT GGAAACAGCG 
CTTTTAGATG ATGAGCCCCA TTGGTATAAA CTCCAGACAC ATGACGAATC TTCACTACCT 
CTGCCTCAGC CATCACCGTT CATGCCCAGG CGGCATATTC ATGGAGAGAG CTCCAGCAAA 
AAGCTACAAA GATCTCAGCG AATCAGTGAT AGTGACATCT CAGATTATGA GGTTGATGAT 
GGTATTGGAG TAGTGCCTCC AGTGGGTTAT AGAGCTAGTG CTAGAGAGAG TAAAGCAACC 
ACGTTAACAG TGCCAGAGCA ACAAAGAACT ACACATCACC GCTCACGTTC CGTGTCTCCT 
CATCGCGGCG ATGATCAGGG AAGGCCTCGT TCACGTTTAC CAAATGTGCC ATTACAGAGG 
AGCTTAGATG AAATTCATCC AACACGAAGG TCACGTTCTC CAACCCGACA CCATGATGCC 
TCCCGAAGCC CGGCCGATCA CAGATCCAGA CATGTGGAAA GTCAATATTC GTCAGAGCCA 
GACAGTGAGC TTCTCATGCT GCCCAGAGCA AAACGAGGAC GAAGTGCAGA AAGCCTACAC 
ATGACCAGTG AACTGCAGCC CTCTCTTGAC AGGGCTAGGA GTGCTAGTAC CAACTGCTTG 
AGACCAGATA CTAGTTTGCA TTCACCAGAA CGAGAAAGGG GTAGATGGTC CCCCTCCCTA 
GATAGGAGGC GACCTGCTAG CCCCAGGATT CAAATCCAGC ATGCATCTCC GGAGAATGAC 
AGGCACTCCA GAAAGTCTGA AAGATGTAGC ATCCAAAAAC AGTCTAGGAA AGGCACAGCC 
TCTGATGCAG ACAGGGTTCT CCCACCATGC CTTTCTAGAA GGGGATACGC AACCCCAAGA 
GCAACCGATC AACCGGTCGT TAGGGGAAAG CATCCCACTC GTTCACGGTC GAGCGAGCAC 
TCTAGTGTCA GAACCCTGTG TTCTATGCAC CACCTTGCCC CCGGAGGGTC GGCGCCACCT 
TCTCCACTTC TGACAAGAAC GCACCGACAA GGAAGCCCAA CCCAGTCTCC TCCAGCAGAC 
ACATCCTTCG GCAGTCGCCG TGGAAGACAG CTCCCACAGG TGCCAGTTCG AAGCGGCAGT 
ATAGAACAAG CAAGCTTAGT AGTGGAGGAG CGAACGAGAC AGATGAAAAT GAAAGTTCAC 
CGATTTAAGC AGACAACAGG GTCTGGGTCT AGTCAAGAAC TTGACCACGA GCAATACTCC 
AAGTACAACA TACATAAAGA TCAGTACAGA AGCTGTGATA ACGCGTCTGC CAAGTCTTCA 
GATAGTGATG TCAGTGATGT GTCCGCCATT TCCAGAGCCA GCAGTACCTC ACGCCTCAGC 
AGCACAAGCT TTATGTCAGA GCAGTCTGAG CGCCCCAGGG GTAGGATCAG TTCATTTACC 
CCCAAAATGC AAGGCAGACG GATGGGGACT TCAGGAAGAG CCATCATCAA GAGCACCAGT 
GTAAGTGGAG AGATATATAC ACTGGAACGT AATGACGGTA GCCAGTCGGA CACGGCCGTG 
GGTACCGTCG GAGCCGGTGG AAAGAAACGA AGATCCAGCC TGAGCGCCAA AGTGGTAGCC 
ATTGTGTCTC GAAGAAGCAG GAGCACGTCA CAGCTCAGCC AGACAGAGTC GGGCCACAAG 
AAGTTGAAAA GCACCATCCA GAGGAGTACG GAAACAGGAA TGGCAGCTGA AATGCGGAAG 
ATGGTGAGAC AGCCGAGCCG GGAGTCCACG GATGGCAGCA TCAACAGTTA TAGCTCGGAA 
GGAAACTTGA TATTTCCTGG AGTTCGAGTA GGACCCGACA GTCAGTTCAG TGATTTCCTT 
GATGGGTTGG GACCAGCGCA GCTCGTTGGC CGTCAGACGC TCGCCACCCC GGCCATGGGC 
GATATCCAAA TCGGGATGGA GGATAAGAAG GGTCAGTTGG AGGTTGAGGT TATCAGAGCC 
CGGAGCCTTA CACAAAAACC TGGTTCCAAA TCTACACCCG CTCCCTATGT GAAAGTATAT 
CTTTTGGAAA ATGGAGCCTG TATTGCCAAA AAGAAGACAA GAATTGCACG GAAAACTCTC 
GATCCTTTGT ATCAGCAGTC CCTGGTTTTT GATGAAAGTC CACAGGGTAA AGTTCTTCAG 
GTGATTGTCT GGGGTGACTA TGGAAGAATG GACCACAAAT GCTTTATGGG TGTGGCTCAA 
ATCTTGTTGG AAGAACTTGA TCTATCCAGC ATGGTGATTG GATGGTATAA ATTGTTCCCT 
CCGTCCTCAC TGGTGGATCC CACTCTCGCT CCCCTGACCC GCCGGGCTTC CCAATCATCT 
CTGGAAAGTT CGTCCGGGCC TCCCTGCATC CGGTCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rims1 ( XM_017596679.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017452168.1 Rattus norvegicus regulating synaptic membrane exocytosis 1 (Rims1), transcript variant X26, mRNA.

Price & Availability↑

CloneID	ORa62896
Clone ID Related Accession (Same CDS sequence)	XM_017596679.1
Accession Version	XM_017596679.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4251bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	regulating synaptic membrane exocytosis protein 1 isoform X26
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005108.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCTCGG CCGTGGGGCC CCGAGGTCCT CGCCCACCCA CGGTGCCTCC CCCTATGCAA 
GAACTGCCCG ACCTGAGCCA CCTGACCGAG GAGGAGAGGA ACATTATCAT GGCAGTGATG 
GACCGGCAGA AGGAAGAGGA GGAAAAAGAA GAGGCCATGC TCAAGAGATT GCATCAACAG 
TTTGAAAGCT ACAAGGAGCA AGTGAGAAAA ATCGGAGAGG AAGCGAGGCG TTACCAGGGC 
GAGCACAAGG ATGATGCCCC GACGTGTGGA ATCTGTCATA AGACAAAGTT TGCTGATGGA 
TGTGGCCATC TCTGCTCCTA TTGTCGCACC AAGTTCTGTG CACGCTGCGG AGGCCGTGTG 
TCTCTGCGAT CGAACAATGT TATGTGGGTA TGCAATTTAT GTCGAAAGCA ACAAGAAATC 
TTAACGAAAT CTGGAGCGTG GTTCTTTGGA AGTGGCCCTC AGCAGCCTAG TCAAGATGGG 
ACTCTGAGTG ACACGGCCAC AGGTGCTGGA TCTGAGGTGC CAAGAGAAAA GAAAGCAAGG 
CTCCAAGAGC GATCAAGGTC TCAGACGCCC TTGAGTACAG CAGCTGTCTC TTCCCAAGAC 
ACTGCTACCC CCGGTGCACC GTTGGACAGG AACAAAGGGG CTGAGCCCTC ACAGCAAGCC 
TTGGGTCCTG AACAGAAGCA GGCATCAAGA TCAAGAAGCG AGCCACCGAG GGAAAGGAAG 
AAGGCTCCAG GGCTTTCAGA GCAGAATGGC AAGGGAGGCC AGAAGAGCGA GCGCAAACGT 
GTCCCCAAGT CTGTGGTGCA ACCCGGGGAA GGGATCGCGG ATGAGAGGGA GAGGAAAGAG 
AGGCGGGAAA CCCGCAGGTT GGAGAAAGGG CGCTCCCAGG ACTACTCAGA CCGGCCTGAG 
AAACGCGACA ATGGCAGGGT GGCGGAAGAC CAGAAGCAGA GGAAGGAGGA GGAGTACCAG 
ACTAGGTACC GCAGCGACCC TAACCTGGCT CGCTACCCGG TGAAGGCGCC GCCAGAGGAG 
CAGCAGATGC GCATGCACGC CCGGGTGTCC CGAGCGAGGC ACGAGCGGCG CCACAGCGAC 
GTGGCGCTCC CGCACACCGA GGCAGCTGCC GCCGCGCCGG CTGAGGCCAC GGCGGGCAAG 
CGCGCGCCGG CCACCGCCAG GGTCTCTCCC CCGGAGTCCC CGCGCGCACG CGCGCCCGCC 
GCCCAGCCTC CCACCGAGCA CGGGCCACCG CCGCCGCGGC CAGTCCCGGG TCCCGCAGAG 
CCACCCGAGC CGCGCGTCCC CGAGCCGCTC CGTAAGCAGG GCCGCCTGGA CCCGGCCTCG 
GCCGTGCTTC TGCGCAAGGC CAAGCGCGAG AAGGCGGAGA GCATGCTGCG GAACGACTCG 
CTGAGCTCCG ATCAGTCCGA GTCCGTGCGG CCATCCCCGC CCAAGCCTCA CCGGCCCAAG 
CGGGGAGGCA AGAGACGTCA GATGTCGGTG AGCAGCTCGG AGGAGGAGGG CGTGTCCACA 
CCGGAGTACA CGAGCTGCGA GGACGTGGAG CTGGAGAGCG AGAGCGTGAG CGAGAAAGGT 
GACTTGGATT ACTACTGGTT GGATCCCGCC ACGTGGCACA GCAGGGAAAC GTCGCCTATC 
AGTTCGCATC CTGTAACGTG GCAGCCGTCT AAAGAGGGAG ATCGACTAAT CGGCCGTGTT 
ATTCTTAACA AAAGAACAAC CATGCCCAAA GAATCAGGTG CATTATTGGG TCTGAAGGTG 
GTTGGAGGAA AAATGACGGA CTTAGGGCGC CTTGGTGCTT TCATCACCAA AGTAAAGAAG 
GGCAGCCTGG CAGACGTCGT CGGACACCTA AGAGCAGGGG ACGAAGTCCT AGAGTGGAAT 
GGTAAACCCC TGCCGGGAGC AACAAACGAA GAAGTTTACA ACATTATCTT AGAATCAAAA 
TCAGAACCTC AAGTTGAGAT TATTGTTTCA AGGCCTATTG GTGACATCCC CAGGATCCCT 
GAGAGTTCCC ATCCTCCCCT GGAGTCCAGT TCAAGTTCCT TTGAATCTCA GAAAATGGAA 
AGGCCTTCTA TTTCTGTTAT TTCTCCAACC AGCCCTGGAG CTCTGAAAGA TGCCCCACAA 
GTCTTACCAG GGCAACTCTC AGTGAAGCTA TGGTATGATA AAGTGGGGCA CCAGCTGATT 
GTAAATGTTC TACAAGCAAC AGATCTACCC CCTAGAGTAG ATGGCCGTCC CAGGAATCCC 
TATGTAAAAA TGTATTTTCT TCCAGATAGA AGCGACAAAA GTAAAAGGAG AACCAAAACA 
GTAAAGAAAC TTCTAGAGCC AAAATGGAAC CAGACATTTG TCTACTCACA CGTACATCGT 
AGAGATTTTC GAGAGCGAAT GTTAGAGATT ACCGTGTGGG ACCAGCCGAG AGTACAGGAC 
GAAGAGAGTG AATTTCTTGG AGAGATCCTC ATAGAGTTGG AAACAGCGCT TTTAGATGAT 
GAGCCCCATT GGTATAAACT CCAGACACAT GACGAATCTT CACTACCTCT GCCTCAGCCA 
TCACCGTTCA TGCCCAGGCG GCATATTCAT GGAGAGAGCT CCAGCAAAAA GCTACAAAGA 
TCTCAGCGAA TCAGTGATAG TGACATCTCA GATTATGAGG TTGATGATGG TATTGGAGTA 
GTGCCTCCAG TGGGTTATAG AGCTAGTGCT AGAGAGAGTA AAGCAACCAC GTTAACAGTG 
CCAGAGCAAC AAAGAACTAC ACATCACCGC TCACGTTCCG TGTCTCCTCA TCGCGGCGAT 
GATCAGGGAA GGCCTCGTTC ACGTTTACCA AATGTGCCAT TACAGAGGAG CTTAGATGAA 
ATTCATCCAA CACGAAGGTC ACGTTCTCCA ACCCGACACC ATGATGCCTC CCGAAGCCCG 
GCCGATCACA GATCCAGACA TGTGGAAAGT CAATATTCGT CAGAGCCAGA CAGTGAGCTT 
CTCATGCTGC CCAGAGCAAA ACGAGGACGA AGTGCAGAAA GCCTACACAT GACCAGTGAA 
CTGCAGCCCT CTCTTGACAG GGCTAGGAGT GCTAGTACCA ACTGCTTGAG ACCAGATACT 
AGTTTGCATT CACCAGAACG AGAAAGGGGT AGATGGTCCC CCTCCCTAGA TAGGAGGCGA 
CCTGCTAGCC CCAGGATTCA AATCCAGCAT GCATCTCCGG AGAATGACAG GCACTCCAGA 
AAGTCTGAAA GATGTAGCAT CCAAAAACAG TCTAGGAAAG GCACAGCCTC TGATGCAGAC 
AGGGTTCTCC CACCATGCCT TTCTAGAAGG GGATACGCAA CCCCAAGAGC AACCGATCAA 
CCGGTCGTTA GGGGAAAGCA TCCCACTCGT TCACGGTCGA GCGAGCACTC TAGTGTCAGA 
ACCCTGTGTT CTATGCACCA CCTTGCCCCC GGAGGGTCGG CGCCACCTTC TCCACTTCTG 
ACAAGAACGC ACCGACAAGG AAGCCCAACC CAGTCTCCTC CAGCAGACAC ATCCTTCGGC 
AGTCGCCGTG GAAGACAGCT CCCACAGGTG CCAGTTCGAA GCGGCAGTAT AGAACAAGAG 
TCGGGCCACA AGAAGTTGAA AAGCACCATC CAGAGGAGTA CGGAAACAGG AATGGCAGCT 
GAAATGCGGA AGATGGTGAG ACAGCCGAGC CGGGAGTCCA CGGATGGCAG CATCAACAGT 
TATAGCTCGG AAGGAAACTT GATATTTCCT GGAGTTCGAG TAGGACCCGA CAGTCAGTTC 
AGTGATTTCC TTGATGGGTT GGGACCAGCG CAGCTCGTTG GCCGTCAGAC GCTCGCCACC 
CCGGCCATGG GCGATATCCA AATCGGGATG GAGGATAAGA AGGGTCAGTT GGAGGTTGAG 
GTTATCAGAG CCCGGAGCCT TACACAAAAA CCTGGTTCCA AATCTACACC CGCTCCCTAT 
GTGAAAGTAT ATCTTTTGGA AAATGGAGCC TGTATTGCCA AAAAGAAGAC AAGAATTGCA 
CGGAAAACTC TCGATCCTTT GTATCAGCAG TCCCTGGTTT TTGATGAAAG TCCACAGGGT 
AAAGTTCTTC AGGTGATTGT CTGGGGTGAC TATGGAAGAA TGGACCACAA ATGCTTTATG 
GGTGTGGCTC AAATCTTGTT GGAAGAACTT GATCTATCCA GCATGGTGAT TGGATGGTAT 
AAATTGTTCC CTCCGTCCTC ACTGGTGGAT CCCACTCTCG CTCCCCTGAC CCGCCGGGCT 
TCCCAATCAT CTCTGGAAAG TTCGTCCGGG CCTCCCTGCA TCCGGTCATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017452168.1
CDS	318..4568
Translation	MSSAVGPRGPRPPTVPPPMQELPDLSHLTEEERNIIMAVMDRQKEEEEKEEAMLKRLHQQFESYKEQVRKIGEEARRYQGEHKDDAPTCGICHKTKFADGCGHLCSYCRTKFCARCGGRVSLRSNNVMWVCNLCRKQQEILTKSGAWFFGSGPQQPSQDGTLSDTATGAGSEVPREKKARLQERSRSQTPLSTAAVSSQDTATPGAPLDRNKGAEPSQQALGPEQKQASRSRSEPPRERKKAPGLSEQNGKGGQKSERKRVPKSVVQPGEGIADERERKERRETRRLEKGRSQDYSDRPEKRDNGRVAEDQKQRKEEEYQTRYRSDPNLARYPVKAPPEEQQMRMHARVSRARHERRHSDVALPHTEAAAAAPAEATAGKRAPATARVSPPESPRARAPAAQPPTEHGPPPPRPVPGPAEPPEPRVPEPLRKQGRLDPASAVLLRKAKREKAESMLRNDSLSSDQSESVRPSPPKPHRPKRGGKRRQMSVSSSEEEGVSTPEYTSCEDVELESESVSEKGDLDYYWLDPATWHSRETSPISSHPVTWQPSKEGDRLIGRVILNKRTTMPKESGALLGLKVVGGKMTDLGRLGAFITKVKKGSLADVVGHLRAGDEVLEWNGKPLPGATNEEVYNIILESKSEPQVEIIVSRPIGDIPRIPESSHPPLESSSSSFESQKMERPSISVISPTSPGALKDAPQVLPGQLSVKLWYDKVGHQLIVNVLQATDLPPRVDGRPRNPYVKMYFLPDRSDKSKRRTKTVKKLLEPKWNQTFVYSHVHRRDFRERMLEITVWDQPRVQDEESEFLGEILIELETALLDDEPHWYKLQTHDESSLPLPQPSPFMPRRHIHGESSSKKLQRSQRISDSDISDYEVDDGIGVVPPVGYRASARESKATTLTVPEQQRTTHHRSRSVSPHRGDDQGRPRSRLPNVPLQRSLDEIHPTRRSRSPTRHHDASRSPADHRSRHVESQYSSEPDSELLMLPRAKRGRSAESLHMTSELQPSLDRARSASTNCLRPDTSLHSPERERGRWSPSLDRRRPASPRIQIQHASPENDRHSRKSERCSIQKQSRKGTASDADRVLPPCLSRRGYATPRATDQPVVRGKHPTRSRSSEHSSVRTLCSMHHLAPGGSAPPSPLLTRTHRQGSPTQSPPADTSFGSRRGRQLPQVPVRSGSIEQESGHKKLKSTIQRSTETGMAAEMRKMVRQPSRESTDGSINSYSSEGNLIFPGVRVGPDSQFSDFLDGLGPAQLVGRQTLATPAMGDIQIGMEDKKGQLEVEVIRARSLTQKPGSKSTPAPYVKVYLLENGACIAKKKTRIARKTLDPLYQQSLVFDESPQGKVLQVIVWGDYGRMDHKCFMGVAQILLEELDLSSMVIGWYKLFPPSSLVDPTLAPLTRRASQSSLESSSGPPCIRS

Target ORF information:

RefSeq Version	XM_017596679.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus regulating synaptic membrane exocytosis 1 (Rims1), transcript variant X26, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017596679.1

ORF Insert Sequence:

ATGTCCTCGG CCGTGGGGCC CCGAGGTCCT CGCCCACCCA CGGTGCCTCC CCCTATGCAA 
GAACTGCCCG ACCTGAGCCA CCTGACCGAG GAGGAGAGGA ACATTATCAT GGCAGTGATG 
GACCGGCAGA AGGAAGAGGA GGAAAAAGAA GAGGCCATGC TCAAGAGATT GCATCAACAG 
TTTGAAAGCT ACAAGGAGCA AGTGAGAAAA ATCGGAGAGG AAGCGAGGCG TTACCAGGGC 
GAGCACAAGG ATGATGCCCC GACGTGTGGA ATCTGTCATA AGACAAAGTT TGCTGATGGA 
TGTGGCCATC TCTGCTCCTA TTGTCGCACC AAGTTCTGTG CACGCTGCGG AGGCCGTGTG 
TCTCTGCGAT CGAACAATGT TATGTGGGTA TGCAATTTAT GTCGAAAGCA ACAAGAAATC 
TTAACGAAAT CTGGAGCGTG GTTCTTTGGA AGTGGCCCTC AGCAGCCTAG TCAAGATGGG 
ACTCTGAGTG ACACGGCCAC AGGTGCTGGA TCTGAGGTGC CAAGAGAAAA GAAAGCAAGG 
CTCCAAGAGC GATCAAGGTC TCAGACGCCC TTGAGTACAG CAGCTGTCTC TTCCCAAGAC 
ACTGCTACCC CCGGTGCACC GTTGGACAGG AACAAAGGGG CTGAGCCCTC ACAGCAAGCC 
TTGGGTCCTG AACAGAAGCA GGCATCAAGA TCAAGAAGCG AGCCACCGAG GGAAAGGAAG 
AAGGCTCCAG GGCTTTCAGA GCAGAATGGC AAGGGAGGCC AGAAGAGCGA GCGCAAACGT 
GTCCCCAAGT CTGTGGTGCA ACCCGGGGAA GGGATCGCGG ATGAGAGGGA GAGGAAAGAG 
AGGCGGGAAA CCCGCAGGTT GGAGAAAGGG CGCTCCCAGG ACTACTCAGA CCGGCCTGAG 
AAACGCGACA ATGGCAGGGT GGCGGAAGAC CAGAAGCAGA GGAAGGAGGA GGAGTACCAG 
ACTAGGTACC GCAGCGACCC TAACCTGGCT CGCTACCCGG TGAAGGCGCC GCCAGAGGAG 
CAGCAGATGC GCATGCACGC CCGGGTGTCC CGAGCGAGGC ACGAGCGGCG CCACAGCGAC 
GTGGCGCTCC CGCACACCGA GGCAGCTGCC GCCGCGCCGG CTGAGGCCAC GGCGGGCAAG 
CGCGCGCCGG CCACCGCCAG GGTCTCTCCC CCGGAGTCCC CGCGCGCACG CGCGCCCGCC 
GCCCAGCCTC CCACCGAGCA CGGGCCACCG CCGCCGCGGC CAGTCCCGGG TCCCGCAGAG 
CCACCCGAGC CGCGCGTCCC CGAGCCGCTC CGTAAGCAGG GCCGCCTGGA CCCGGCCTCG 
GCCGTGCTTC TGCGCAAGGC CAAGCGCGAG AAGGCGGAGA GCATGCTGCG GAACGACTCG 
CTGAGCTCCG ATCAGTCCGA GTCCGTGCGG CCATCCCCGC CCAAGCCTCA CCGGCCCAAG 
CGGGGAGGCA AGAGACGTCA GATGTCGGTG AGCAGCTCGG AGGAGGAGGG CGTGTCCACA 
CCGGAGTACA CGAGCTGCGA GGACGTGGAG CTGGAGAGCG AGAGCGTGAG CGAGAAAGGT 
GACTTGGATT ACTACTGGTT GGATCCCGCC ACGTGGCACA GCAGGGAAAC GTCGCCTATC 
AGTTCGCATC CTGTAACGTG GCAGCCGTCT AAAGAGGGAG ATCGACTAAT CGGCCGTGTT 
ATTCTTAACA AAAGAACAAC CATGCCCAAA GAATCAGGTG CATTATTGGG TCTGAAGGTG 
GTTGGAGGAA AAATGACGGA CTTAGGGCGC CTTGGTGCTT TCATCACCAA AGTAAAGAAG 
GGCAGCCTGG CAGACGTCGT CGGACACCTA AGAGCAGGGG ACGAAGTCCT AGAGTGGAAT 
GGTAAACCCC TGCCGGGAGC AACAAACGAA GAAGTTTACA ACATTATCTT AGAATCAAAA 
TCAGAACCTC AAGTTGAGAT TATTGTTTCA AGGCCTATTG GTGACATCCC CAGGATCCCT 
GAGAGTTCCC ATCCTCCCCT GGAGTCCAGT TCAAGTTCCT TTGAATCTCA GAAAATGGAA 
AGGCCTTCTA TTTCTGTTAT TTCTCCAACC AGCCCTGGAG CTCTGAAAGA TGCCCCACAA 
GTCTTACCAG GGCAACTCTC AGTGAAGCTA TGGTATGATA AAGTGGGGCA CCAGCTGATT 
GTAAATGTTC TACAAGCAAC AGATCTACCC CCTAGAGTAG ATGGCCGTCC CAGGAATCCC 
TATGTAAAAA TGTATTTTCT TCCAGATAGA AGCGACAAAA GTAAAAGGAG AACCAAAACA 
GTAAAGAAAC TTCTAGAGCC AAAATGGAAC CAGACATTTG TCTACTCACA CGTACATCGT 
AGAGATTTTC GAGAGCGAAT GTTAGAGATT ACCGTGTGGG ACCAGCCGAG AGTACAGGAC 
GAAGAGAGTG AATTTCTTGG AGAGATCCTC ATAGAGTTGG AAACAGCGCT TTTAGATGAT 
GAGCCCCATT GGTATAAACT CCAGACACAT GACGAATCTT CACTACCTCT GCCTCAGCCA 
TCACCGTTCA TGCCCAGGCG GCATATTCAT GGAGAGAGCT CCAGCAAAAA GCTACAAAGA 
TCTCAGCGAA TCAGTGATAG TGACATCTCA GATTATGAGG TTGATGATGG TATTGGAGTA 
GTGCCTCCAG TGGGTTATAG AGCTAGTGCT AGAGAGAGTA AAGCAACCAC GTTAACAGTG 
CCAGAGCAAC AAAGAACTAC ACATCACCGC TCACGTTCCG TGTCTCCTCA TCGCGGCGAT 
GATCAGGGAA GGCCTCGTTC ACGTTTACCA AATGTGCCAT TACAGAGGAG CTTAGATGAA 
ATTCATCCAA CACGAAGGTC ACGTTCTCCA ACCCGACACC ATGATGCCTC CCGAAGCCCG 
GCCGATCACA GATCCAGACA TGTGGAAAGT CAATATTCGT CAGAGCCAGA CAGTGAGCTT 
CTCATGCTGC CCAGAGCAAA ACGAGGACGA AGTGCAGAAA GCCTACACAT GACCAGTGAA 
CTGCAGCCCT CTCTTGACAG GGCTAGGAGT GCTAGTACCA ACTGCTTGAG ACCAGATACT 
AGTTTGCATT CACCAGAACG AGAAAGGGGT AGATGGTCCC CCTCCCTAGA TAGGAGGCGA 
CCTGCTAGCC CCAGGATTCA AATCCAGCAT GCATCTCCGG AGAATGACAG GCACTCCAGA 
AAGTCTGAAA GATGTAGCAT CCAAAAACAG TCTAGGAAAG GCACAGCCTC TGATGCAGAC 
AGGGTTCTCC CACCATGCCT TTCTAGAAGG GGATACGCAA CCCCAAGAGC AACCGATCAA 
CCGGTCGTTA GGGGAAAGCA TCCCACTCGT TCACGGTCGA GCGAGCACTC TAGTGTCAGA 
ACCCTGTGTT CTATGCACCA CCTTGCCCCC GGAGGGTCGG CGCCACCTTC TCCACTTCTG 
ACAAGAACGC ACCGACAAGG AAGCCCAACC CAGTCTCCTC CAGCAGACAC ATCCTTCGGC 
AGTCGCCGTG GAAGACAGCT CCCACAGGTG CCAGTTCGAA GCGGCAGTAT AGAACAAGAG 
TCGGGCCACA AGAAGTTGAA AAGCACCATC CAGAGGAGTA CGGAAACAGG AATGGCAGCT 
GAAATGCGGA AGATGGTGAG ACAGCCGAGC CGGGAGTCCA CGGATGGCAG CATCAACAGT 
TATAGCTCGG AAGGAAACTT GATATTTCCT GGAGTTCGAG TAGGACCCGA CAGTCAGTTC 
AGTGATTTCC TTGATGGGTT GGGACCAGCG CAGCTCGTTG GCCGTCAGAC GCTCGCCACC 
CCGGCCATGG GCGATATCCA AATCGGGATG GAGGATAAGA AGGGTCAGTT GGAGGTTGAG 
GTTATCAGAG CCCGGAGCCT TACACAAAAA CCTGGTTCCA AATCTACACC CGCTCCCTAT 
GTGAAAGTAT ATCTTTTGGA AAATGGAGCC TGTATTGCCA AAAAGAAGAC AAGAATTGCA 
CGGAAAACTC TCGATCCTTT GTATCAGCAG TCCCTGGTTT TTGATGAAAG TCCACAGGGT 
AAAGTTCTTC AGGTGATTGT CTGGGGTGAC TATGGAAGAA TGGACCACAA ATGCTTTATG 
GGTGTGGCTC AAATCTTGTT GGAAGAACTT GATCTATCCA GCATGGTGAT TGGATGGTAT 
AAATTGTTCC CTCCGTCCTC ACTGGTGGAT CCCACTCTCG CTCCCCTGAC CCGCCGGGCT 
TCCCAATCAT CTCTGGAAAG TTCGTCCGGG CCTCCCTGCA TCCGGTCATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Fusion Competent Synaptic Vesicles Persist upon Active Zone Disruption and Loss of Vesicle Docking.
Neuron91(4)777-791(2016 Aug)
Wang SSH,Held RG,Wong MY,Liu C,Karakhanyan A,Kaeser PS

A trans-synaptic nanocolumn aligns neurotransmitter release to receptors.
Nature536(7615)210-4(2016 08)
Tang AH,Chen H,Li TP,Metzbower SR,MacGillavry HD,Blanpied TA

Protein mutated in paroxysmal dyskinesia interacts with the active zone protein RIM and suppresses synaptic vesicle exocytosis.
Proceedings of the National Academy of Sciences of the United States of America112(10)2935-41(2015 Mar)
Shen Y,Ge WP,Li Y,Hirano A,Lee HY,Rohlmann A,Missler M,Tsien RW,Jan LY,Fu YH,Pt??ek LJ

RIM1? SUMOylation is required for fast synaptic vesicle exocytosis.
Cell reports5(5)1294-301(2013 Dec)
Girach F,Craig TJ,Rocca DL,Henley JM

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Rims1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

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