gRNAs
The following Sf3b1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Sf3b1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
| CloneID | ORa09534 | |
| Clone ID Related Accession (Same CDS sequence) | NM_053426.1 | |
| Accession Version | NM_053426.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 3915bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector |  User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-10-09 | |
| Organism | Rattus norvegicus(Norway rat) | |
| Product | splicing factor 3B subunit 1 | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AABR07067758.1. On or before Jun 5, 2008 this sequence version replaced XM_343570.3, XM_001066744.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END## | |
1 | ATGGCGAAGA TCGCCAAGAC TCACGAAGAT ATCGAAGCAC AGATTCGAGA AATTCAAGGC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_445878.1 |
| CDS | 130..4044 |
| Translation |
Target ORF information:
Target ORF information:
|
 NM_053426.1 |
1 | ATGGCGAAGA TCGCCAAGAC TCACGAAGAT ATCGAAGCAC AGATTCGAGA AATTCAAGGC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | ORa32212 | |
| Clone ID Related Accession (Same CDS sequence) | XM_017596654.1 | |
| Accession Version | XM_017596654.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 3477bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2016-07-25 | |
| Organism | Rattus norvegicus(Norway rat) | |
| Product | splicing factor 3B subunit 1 isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005108.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGAATGCTA GAACCTACAT GGATGTTATG CGAGAACAAC ATTTGACTAA GGAAGAGAGA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_017452143.1 |
| CDS | 1473..4949 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_017596654.1 |
1 | ATGAATGCTA GAACCTACAT GGATGTTATG CGAGAACAAC ATTTGACTAA GGAAGAGAGA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
Structure of the human activated spliceosome in three conformational states. |
Cryo-EM Structure of a Pre-catalytic Human Spliceosome Primed for Activation. |
Splicing modulators act at the branch point adenosine binding pocket defined by the PHF5A-SF3b complex. |
Towards Functional Annotation of the Preimplantation Transcriptome: An RNAi Screen in Mammalian Embryos. |
Molecular Architecture of SF3b and Structural Consequences of Its Cancer-Related Mutations. |
