Slc4a4 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Slc4a4
Entrez Gene ID	84484
Full Name	solute carrier family 4 member 4
Synonyms	HHNMC,HNBC1,KNBC,NBC2,Nbc1,Nbc4,PNBC,SLC4A5
General protein information	Preferred Names solute carrier family 4 member 4 Names electrogenic sodium bicarbonate cotransporter 1 NBC-like protein Na(+)/HCO3(-) cotransporter Na+HCO3- cotransporter 4 sodium bicarbonate cotransporter solute carrier family 4 (anion exchanger), member 4 solute carrier family 4, sodium bicarbonate cotransporter, member 4
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 14 Map Location: 14p22
Summary	sodium bicarbonate cotransporter; important for sodium and bicarbonate transport across membranes [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_017599396.1	XP_017454885.1	electrogenic sodium bicarbonate cotransporter 1 isoform X1
NM_053424.1	NP_445876.1	electrogenic sodium bicarbonate cotransporter 1
XM_006250791.3	XP_006250853.1	electrogenic sodium bicarbonate cotransporter 1 isoform X2

Pathways from BioSystems

Homologies

Bos taurus (cattle)	SLC4A4	NP_777030.1
Neurospora crassa	NCU01480	XP_956757.2
Pan troglodytes (chimpanzee)	SLC4A4	XP_517243.2
Saccharomyces cerevisiae (baker's yeast)	BOR1	NP_014124.1
Arabidopsis thaliana (thale cress)	BOR5	NP_177619.2
Canis lupus familiaris (dog)	SLC4A4	XP_003431880.1
Kluyveromyces lactis	KLLA0C02123g	XP_452291.1
	SLC4A4	XP_001104906.1
Mus musculus (house mouse)	Slc4a4	NP_061230.2
Rattus norvegicus (Norway rat)	Slc4a4	NP_445876.1
Gallus gallus (chicken)	SLC4A4	XP_420603.3
Schizosaccharomyces pombe (fission yeast)	SPBC543.05c	NP_596793.1
Magnaporthe oryzae (rice blast fungus)	MGG_15203	XP_003711328.1
Arabidopsis thaliana (thale cress)	BOR4	NP_172999.1
Xenopus tropicalis (tropical clawed frog)	slc4a4	XP_004919190.1
Homo sapiens (human)	SLC4A4	NP_001091954.1
Danio rerio (zebrafish)	slc4a4a	NP_001030156.1
Eremothecium gossypii	AGOS_AFR677C	NP_986225.2

Gene Ontology
Bibliography

Related articles in PubMed

Modulation of oxidative and glycolytic skeletal muscle fibers Na+/H+ exchanger1 (NHE1) and Na+/HCO3- co-transporter1 (NBC1) genes and proteins expression in type 2 diabetic rat (Streptozotocin + high fat diet) following long term endurance training.
Monazzami A, Rajabi H, Ghrakhanlou R, Yari K, Rahimi Z
Cellular and molecular biology (Noisy-le-Grand, France)63(5)11-18(2017 May)

Na
Cao LH, Xia CC, Shi ZC, Wang N, Gu ZH, Yu LZ, Wan Q, De W
World journal of gastroenterology22(43)9525-9533(2016 Nov)

The cardiac electrogenic sodium/bicarbonate cotransporter (NBCe1) is activated by aldosterone through the G protein-coupled receptor 30 (GPR 30).
Orlowski A, De Giusti VC, Ciancio MC, Espejo MS, Aiello EA
Channels (Austin, Tex.)10(5)428-434(2016 Sep)

Aldosterone stimulates the cardiac sodium/bicarbonate cotransporter via activation of the g protein-coupled receptor gpr30.
De Giusti VC, Orlowski A, Ciancio MC, Espejo MS, Gonano LA, Caldiz CI, Vila Petroff MG, Villa-Abrille MC, Aiello EA
Journal of molecular and cellular cardiology89(Pt B)260-7(2015 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

NBC1 is diversely expressed in the pancreas at different developmental stages, where it may exert its functions in pancreatic development, especially islet cell growth, through HCO3(-) transport and pH regulation.
Title: Na

NBCe1 is the NBC isoform activated by aldosterone in the heart
Title: The cardiac electrogenic sodium/bicarbonate cotransporter (NBCe1) is activated by aldosterone through the G protein-coupled receptor 30 (GPR 30).

Aldosterone stimulates NBC by GPR30 activation, reactive oxygen species production and AKT stimulation.
Title: Aldosterone stimulates the cardiac sodium/bicarbonate cotransporter via activation of the g protein-coupled receptor gpr30.

Results demonstrate that transcriptional regulation and targeting of NBCe1, as well as functional operation of NBCe1, may occur through multiple signaling pathways
Title: Regulation of functional expression of the electrogenic sodium bicarbonate cotransporter 1, NBCe1 (SLC4A4), in mouse astrocytes.

These results revealed that insulin can stimulate PT sodium transport even in type 2 diabetes with overt nephropathy.
Title: Stimulatory effect of insulin on renal proximal tubule sodium transport is preserved in type 2 diabetes with nephropathy.

The following Slc4a4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Slc4a4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa58438	XM_017599396.1 Latest version!	Rattus norvegicus solute carrier family 4 member 4 (Slc4a4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ORa09523	NM_053424.1 Latest version!	Rattus norvegicus solute carrier family 4 member 4 (Slc4a4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
ORa35943	XM_006250791.3 Latest version!	Rattus norvegicus solute carrier family 4 member 4 (Slc4a4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Slc4a4 ( XM_017599396.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017454885.1 Rattus norvegicus solute carrier family 4 member 4 (Slc4a4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa58438
Clone ID Related Accession (Same CDS sequence)	XM_017599396.1
Accession Version	XM_017599396.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3456bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	electrogenic sodium bicarbonate cotransporter 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005113.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGCTCGT ATGAGGGTAT ACATTTTGTG CAGTGGGTGT TTAAAGAGCT AAGCACTGAA 
ACTGTATTTC CACAAACGAC TGCCACGAGC ACTCCAGACA CACCGAACCT CCTCTCTTGG 
ATGGAGGATG AAGCTGTCCT GGACAGAGGG GCTTCCTTCC TTAAACATGT GTGTGACGAA 
GAAGAAGTAG AAGGTCACCA CACGATCTAC ATTGGGGTCC ATGTGCCCAA GAGCTACCGG 
AGAAGGAGAC GCCATAAGAG AAAGGCTGGG CACAAGGAAA AGAAGGAGAA GGAAAGGATC 
TCTGAGAACT ATTCCGACAA ATCAGATGTG GAGAATGCGG ACGAATCCAG CAGCAGCATC 
CTCAAGCCGC TCATCTCCCC GGCCGCAGAA CGTATCCGAT TCATCTTGGG AGAGGAAGAT 
GACAGCCCAG CACCTCCTCA GCTCTTCACG GAACTCGATG AGCTGCTGGC TGTGGATGGA 
CAGGAGATGG AATGGAAGGA GACAGCCAGG TGGATTAAGT TTGAAGAGAA AGTGGAACAG 
GGTGGGGAGC GATGGAGCAA ACCCCATGTG GCCACCTTGT CCCTGCACAG CTTGTTTGAG 
CTGAGGACAT GTATGGAGAA AGGATCCATC ATGCTTGACC GGGAGGCCTC CTCTCTCCCA 
CAGCTGGTGG AGATGATTGC AGACCACCAG ATCGAGACAG GCCTCTTGAA GCCCGACCTC 
AAGGATAAGG TCACCTATAC GCTGCTGCGG AAACACCGAC ATCAAACCAA GAAATCCAAC 
CTTCGGTCCC TGGCTGACAT TGGGAAGACA GTCTCCAGTG CAAGTAGGAT GTTCAGCAAC 
CCTGATAACG CCCGCAGCCC GCTTGAGAAC TCAGTGCCCT GCCTAGCAAA TCGCACCTTG 
GACGATGACC CCCGAGTGCG GCGCACTCCC AGCGTGGGAT GGCTCAGTAG CCCAGCTATG 
ACCCACAGGA ATCTGACATC CTCCAGTCTC AATGACATTT CTGATAAACC AGAGAAGGAC 
CAGCTGAAGA ATAAATTCAT GAAAAAACTG CCCCGTGATG CAGAAGCTTC CAACGTGCTC 
GTTGGGGAGG TTGACTTCTT GGACACTCCC TTCATTGCCT TTGTTCGTCT ACAGCAGGCT 
GTCATGCTGG GTGCCCTGAC TGAGGTGCCT GTGCCCACAA GGTTCTTGTT CATTCTCTTA 
GGTCCAAAGG GGAAAGCCAA GTCCTACCAT GAGATCGGAA GAGCCATCGC CACCTTGATG 
TCTGACGAGG TGTTCCACGA TATCGCTTAC AAAGCGAAAG ATAGACACGA CCTGATTGCT 
GGCATTGACG AGTTCTTAGA TGAAGTCATT GTCCTTCCAC CTGGGGAATG GGACCCGGCA 
ATCAGAATAG AACCTCCAAA GAGCCTCCCA TCATCTGACA AAAGGAAGAA TATGTACTCA 
GGTGGAGAGA ACGTTCAGAT GAATGGGGAC ACACCTCATG ATGGAGGCCA CGGAGGAGGA 
GGCCACGGTG ACTGTGAAGA ACTACAGAGA ACTGGCCGGT TCTGTGGTGG ATTAATTAAG 
GACATAAAGA GGAAAGCACC ATTTTTTGCC AGTGACTTTT ATGACGCTTT AAATATTCAG 
GCTCTCTCGG CAATTCTCTT CATCTATCTG GCGACGGTAA CCAACGCCAT CACTTTCGGA 
GGCCTGCTCG GGGATGCCAC TGACAACATG CAGGGTGTGT TGGAGAGTTT CCTGGGCACT 
GCTGTCTCTG GCGCCATCTT TTGCCTTTTT GCGGGTCAAC CGCTCACCAT CTTGAGTAGC 
ACGGGACCTG TTCTGGTGTT TGAGAGGCTT CTTTTCAACT TCAGCAAGGA CCATAGTTTT 
GACTACTTGG AGTTTCGTCT TTGGATTGGC CTGTGGTCAG CCTTCATGTG TCTCATCCTG 
GTGGCCACTG ATGCCAGCTT CCTGGTTCAG TACTTCACCC GTTTCACGGA AGAAGGATTC 
TCCTCTCTGA TCAGCTTCAT CTTCATCTAT GATGCTTTCA AGAAGATGAT CAAGCTGGCA 
GATTACTATC CCATCAACTC TGACTTCAGA GTGGGCTACA ACACTCACTT CTCCTGTGCT 
TGCCTGCCAC CTGACCCAGT TAATCTCTCA GTATCTAATG ATACCACACT AGCCCCAGAG 
GACCTGCCAA CCGTTTCTTC TACTGACATG TACCACAATG CTACCTTTGA CTGGGCCTAC 
TTGTCAAAGA AAGAGTGTGT GAAATTTGGA GGAAAGCTCG TGGGAAACAA CTGTGACTTC 
GTGCCTGACA TCACACTCAT GTCCTTCATC CTCTTCCTGG GCACTTACAC CTCTTCTATG 
GCCATGAAGA AATTCAAAAC CAGCCGCTAT TTTCCAACCA CAGCAAGAAA ACTGATCAGC 
GATTTTGCCA TCATCCTGTC CATTCTCATA TTCTGTGTAA TAGACGCCCT CGTCGGTGTG 
GATACTCCGA AGCTAATTGT GCCAAGTGAG TTCAAGCCAA CAAGCCCTCA CCGGGGTTGG 
TTTGTCCCAC CCTTTGGAGG AAACCCCTGG TGGGTGTGCC TTGCTGCTGC TATCCCGGCT 
TTGCTAGTCA CCATCCTGAT TTTCATGGAC CAGCAAATCA CGGCCGTGAT TGTGAACAGG 
AAAGAACATA AGCTCAAGAA GGGAGCTGGC TATCACCTGG ACCTGTTTTG GGTCGCCATC 
CTCATGGTGG TGTGCTCCTT CATGGCTCTT CCCTGGTATG TGGCTGCTAC TGTCATCTCC 
ATCGCTCACA TCGACAGTCT GAAGATGGAG ACAGAGACTT CTGCGCCTGG AGAACAACCA 
AAGTTTCTGG GAGTGAGAGA ACAACGAGTC ACTGGAACCC TTGTGTTTAT TCTGACTGGC 
CTGTCAGTCT TTATGGCTCC CATCTTGAAG TTTATCCCCA TGCCTGTTCT GTATGGCGTG 
TTCTTGTATA TGGGGGTGGC CTCACTGAAC GGTGTGCAGT TCATGGACCG TCTTAAGCTG 
CTTCTCATGC CCCTGAAGCA TCAACCTGAC TTCATCTACC TGCGCCACGT CCCCCTGCGC 
CGAGTCCACC TGTTCACCTC CCTGCAGGTG TTGTGCCTGG CTCTGCTCTG GATCCTCAAG 
TCCACAGTGG CTGCGATTAT TTTTCCAGTT ATGATCCTGG CCCTGGTAGC TGTCAGAAAA 
GGTATGGACT ACCTCTTCTC CCAGCACGAC CTCAGCTTCC TTGATGATGT CATTCCAGAG 
AAGGACAAGA AAAAGAAGGA GGATGAGAAG AAGAAGAAAA AGAAGAAAGG AAGTCTGGAT 
AGCGACAACG ACGATTCTGA CTGCCCATAC TCAGAAAAGG TCCCCAGTAT TAAAATTCCA 
ATGGACATCA CGGAACAGCA ACCTTTCCTA AGTGATAACA AACCCTTGGA CAGAGAAAGA 
TCCTCAACAT TCCTTGAACG CCACACATCA TGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017454885.1
CDS	111..3566
Translation	MRSYEGIHFVQWVFKELSTETVFPQTTATSTPDTPNLLSWMEDEAVLDRGASFLKHVCDEEEVEGHHTIYIGVHVPKSYRRRRRHKRKAGHKEKKEKERISENYSDKSDVENADESSSSILKPLISPAAERIRFILGEEDDSPAPPQLFTELDELLAVDGQEMEWKETARWIKFEEKVEQGGERWSKPHVATLSLHSLFELRTCMEKGSIMLDREASSLPQLVEMIADHQIETGLLKPDLKDKVTYTLLRKHRHQTKKSNLRSLADIGKTVSSASRMFSNPDNARSPLENSVPCLANRTLDDDPRVRRTPSVGWLSSPAMTHRNLTSSSLNDISDKPEKDQLKNKFMKKLPRDAEASNVLVGEVDFLDTPFIAFVRLQQAVMLGALTEVPVPTRFLFILLGPKGKAKSYHEIGRAIATLMSDEVFHDIAYKAKDRHDLIAGIDEFLDEVIVLPPGEWDPAIRIEPPKSLPSSDKRKNMYSGGENVQMNGDTPHDGGHGGGGHGDCEELQRTGRFCGGLIKDIKRKAPFFASDFYDALNIQALSAILFIYLATVTNAITFGGLLGDATDNMQGVLESFLGTAVSGAIFCLFAGQPLTILSSTGPVLVFERLLFNFSKDHSFDYLEFRLWIGLWSAFMCLILVATDASFLVQYFTRFTEEGFSSLISFIFIYDAFKKMIKLADYYPINSDFRVGYNTHFSCACLPPDPVNLSVSNDTTLAPEDLPTVSSTDMYHNATFDWAYLSKKECVKFGGKLVGNNCDFVPDITLMSFILFLGTYTSSMAMKKFKTSRYFPTTARKLISDFAIILSILIFCVIDALVGVDTPKLIVPSEFKPTSPHRGWFVPPFGGNPWWVCLAAAIPALLVTILIFMDQQITAVIVNRKEHKLKKGAGYHLDLFWVAILMVVCSFMALPWYVAATVISIAHIDSLKMETETSAPGEQPKFLGVREQRVTGTLVFILTGLSVFMAPILKFIPMPVLYGVFLYMGVASLNGVQFMDRLKLLLMPLKHQPDFIYLRHVPLRRVHLFTSLQVLCLALLWILKSTVAAIIFPVMILALVAVRKGMDYLFSQHDLSFLDDVIPEKDKKKKEDEKKKKKKKGSLDSDNDDSDCPYSEKVPSIKIPMDITEQQPFLSDNKPLDRERSSTFLERHTSC

Target ORF information:

RefSeq Version	XM_017599396.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus solute carrier family 4 member 4 (Slc4a4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017599396.1

ORF Insert Sequence:

ATGCGCTCGT ATGAGGGTAT ACATTTTGTG CAGTGGGTGT TTAAAGAGCT AAGCACTGAA 
ACTGTATTTC CACAAACGAC TGCCACGAGC ACTCCAGACA CACCGAACCT CCTCTCTTGG 
ATGGAGGATG AAGCTGTCCT GGACAGAGGG GCTTCCTTCC TTAAACATGT GTGTGACGAA 
GAAGAAGTAG AAGGTCACCA CACGATCTAC ATTGGGGTCC ATGTGCCCAA GAGCTACCGG 
AGAAGGAGAC GCCATAAGAG AAAGGCTGGG CACAAGGAAA AGAAGGAGAA GGAAAGGATC 
TCTGAGAACT ATTCCGACAA ATCAGATGTG GAGAATGCGG ACGAATCCAG CAGCAGCATC 
CTCAAGCCGC TCATCTCCCC GGCCGCAGAA CGTATCCGAT TCATCTTGGG AGAGGAAGAT 
GACAGCCCAG CACCTCCTCA GCTCTTCACG GAACTCGATG AGCTGCTGGC TGTGGATGGA 
CAGGAGATGG AATGGAAGGA GACAGCCAGG TGGATTAAGT TTGAAGAGAA AGTGGAACAG 
GGTGGGGAGC GATGGAGCAA ACCCCATGTG GCCACCTTGT CCCTGCACAG CTTGTTTGAG 
CTGAGGACAT GTATGGAGAA AGGATCCATC ATGCTTGACC GGGAGGCCTC CTCTCTCCCA 
CAGCTGGTGG AGATGATTGC AGACCACCAG ATCGAGACAG GCCTCTTGAA GCCCGACCTC 
AAGGATAAGG TCACCTATAC GCTGCTGCGG AAACACCGAC ATCAAACCAA GAAATCCAAC 
CTTCGGTCCC TGGCTGACAT TGGGAAGACA GTCTCCAGTG CAAGTAGGAT GTTCAGCAAC 
CCTGATAACG CCCGCAGCCC GCTTGAGAAC TCAGTGCCCT GCCTAGCAAA TCGCACCTTG 
GACGATGACC CCCGAGTGCG GCGCACTCCC AGCGTGGGAT GGCTCAGTAG CCCAGCTATG 
ACCCACAGGA ATCTGACATC CTCCAGTCTC AATGACATTT CTGATAAACC AGAGAAGGAC 
CAGCTGAAGA ATAAATTCAT GAAAAAACTG CCCCGTGATG CAGAAGCTTC CAACGTGCTC 
GTTGGGGAGG TTGACTTCTT GGACACTCCC TTCATTGCCT TTGTTCGTCT ACAGCAGGCT 
GTCATGCTGG GTGCCCTGAC TGAGGTGCCT GTGCCCACAA GGTTCTTGTT CATTCTCTTA 
GGTCCAAAGG GGAAAGCCAA GTCCTACCAT GAGATCGGAA GAGCCATCGC CACCTTGATG 
TCTGACGAGG TGTTCCACGA TATCGCTTAC AAAGCGAAAG ATAGACACGA CCTGATTGCT 
GGCATTGACG AGTTCTTAGA TGAAGTCATT GTCCTTCCAC CTGGGGAATG GGACCCGGCA 
ATCAGAATAG AACCTCCAAA GAGCCTCCCA TCATCTGACA AAAGGAAGAA TATGTACTCA 
GGTGGAGAGA ACGTTCAGAT GAATGGGGAC ACACCTCATG ATGGAGGCCA CGGAGGAGGA 
GGCCACGGTG ACTGTGAAGA ACTACAGAGA ACTGGCCGGT TCTGTGGTGG ATTAATTAAG 
GACATAAAGA GGAAAGCACC ATTTTTTGCC AGTGACTTTT ATGACGCTTT AAATATTCAG 
GCTCTCTCGG CAATTCTCTT CATCTATCTG GCGACGGTAA CCAACGCCAT CACTTTCGGA 
GGCCTGCTCG GGGATGCCAC TGACAACATG CAGGGTGTGT TGGAGAGTTT CCTGGGCACT 
GCTGTCTCTG GCGCCATCTT TTGCCTTTTT GCGGGTCAAC CGCTCACCAT CTTGAGTAGC 
ACGGGACCTG TTCTGGTGTT TGAGAGGCTT CTTTTCAACT TCAGCAAGGA CCATAGTTTT 
GACTACTTGG AGTTTCGTCT TTGGATTGGC CTGTGGTCAG CCTTCATGTG TCTCATCCTG 
GTGGCCACTG ATGCCAGCTT CCTGGTTCAG TACTTCACCC GTTTCACGGA AGAAGGATTC 
TCCTCTCTGA TCAGCTTCAT CTTCATCTAT GATGCTTTCA AGAAGATGAT CAAGCTGGCA 
GATTACTATC CCATCAACTC TGACTTCAGA GTGGGCTACA ACACTCACTT CTCCTGTGCT 
TGCCTGCCAC CTGACCCAGT TAATCTCTCA GTATCTAATG ATACCACACT AGCCCCAGAG 
GACCTGCCAA CCGTTTCTTC TACTGACATG TACCACAATG CTACCTTTGA CTGGGCCTAC 
TTGTCAAAGA AAGAGTGTGT GAAATTTGGA GGAAAGCTCG TGGGAAACAA CTGTGACTTC 
GTGCCTGACA TCACACTCAT GTCCTTCATC CTCTTCCTGG GCACTTACAC CTCTTCTATG 
GCCATGAAGA AATTCAAAAC CAGCCGCTAT TTTCCAACCA CAGCAAGAAA ACTGATCAGC 
GATTTTGCCA TCATCCTGTC CATTCTCATA TTCTGTGTAA TAGACGCCCT CGTCGGTGTG 
GATACTCCGA AGCTAATTGT GCCAAGTGAG TTCAAGCCAA CAAGCCCTCA CCGGGGTTGG 
TTTGTCCCAC CCTTTGGAGG AAACCCCTGG TGGGTGTGCC TTGCTGCTGC TATCCCGGCT 
TTGCTAGTCA CCATCCTGAT TTTCATGGAC CAGCAAATCA CGGCCGTGAT TGTGAACAGG 
AAAGAACATA AGCTCAAGAA GGGAGCTGGC TATCACCTGG ACCTGTTTTG GGTCGCCATC 
CTCATGGTGG TGTGCTCCTT CATGGCTCTT CCCTGGTATG TGGCTGCTAC TGTCATCTCC 
ATCGCTCACA TCGACAGTCT GAAGATGGAG ACAGAGACTT CTGCGCCTGG AGAACAACCA 
AAGTTTCTGG GAGTGAGAGA ACAACGAGTC ACTGGAACCC TTGTGTTTAT TCTGACTGGC 
CTGTCAGTCT TTATGGCTCC CATCTTGAAG TTTATCCCCA TGCCTGTTCT GTATGGCGTG 
TTCTTGTATA TGGGGGTGGC CTCACTGAAC GGTGTGCAGT TCATGGACCG TCTTAAGCTG 
CTTCTCATGC CCCTGAAGCA TCAACCTGAC TTCATCTACC TGCGCCACGT CCCCCTGCGC 
CGAGTCCACC TGTTCACCTC CCTGCAGGTG TTGTGCCTGG CTCTGCTCTG GATCCTCAAG 
TCCACAGTGG CTGCGATTAT TTTTCCAGTT ATGATCCTGG CCCTGGTAGC TGTCAGAAAA 
GGTATGGACT ACCTCTTCTC CCAGCACGAC CTCAGCTTCC TTGATGATGT CATTCCAGAG 
AAGGACAAGA AAAAGAAGGA GGATGAGAAG AAGAAGAAAA AGAAGAAAGG AAGTCTGGAT 
AGCGACAACG ACGATTCTGA CTGCCCATAC TCAGAAAAGG TCCCCAGTAT TAAAATTCCA 
ATGGACATCA CGGAACAGCA ACCTTTCCTA AGTGATAACA AACCCTTGGA CAGAGAAAGA 
TCCTCAACAT TCCTTGAACG CCACACATCA TGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Slc4a4 ( NM_053424.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_445876.1 Rattus norvegicus solute carrier family 4 member 4 (Slc4a4), mRNA.

Price & Availability↑

CloneID	ORa09523
Clone ID Related Accession (Same CDS sequence)	NM_053424.1
Accession Version	NM_053424.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3240bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-27
Organism	Rattus norvegicus(Norway rat)
Product	electrogenic sodium bicarbonate cotransporter 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF254802.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF254802.1, AF210250.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGGAGGATG AAGCTGTCCT GGACAGAGGG GCTTCCTTCC TTAAACATGT GTGTGACGAA 
GAAGAAGTAG AAGGTCACCA CACGATCTAC ATTGGGGTCC ATGTGCCCAA GAGCTACCGG 
AGAAGGAGAC GCCATAAGAG AAAGGCTGGG CACAAGGAAA AGAAGGAGAA GGAAAGGATC 
TCTGAGAACT ATTCCGACAA ATCAGATGTG GAGAATGCGG ACGAATCCAG CAGCAGCATC 
CTCAAGCCGC TCATCTCCCC GGCCGCAGAA CGTATCCGAT TCATCTTGGG AGAGGAAGAT 
GACAGCCCAG CACCTCCTCA GCTCTTCACG GAACTCGATG AGCTGCTGGC TGTGGATGGA 
CAGGAGATGG AATGGAAGGA GACAGCCAGG TGGATTAAGT TTGAAGAGAA AGTGGAACAG 
GGTGGGGAGC GATGGAGCAA ACCCCATGTG GCCACCTTGT CCCTGCACAG CTTGTTTGAG 
CTGAGGACAT GTATGGAGAA AGGATCCATC ATGCTTGACC GGGAGGCCTC CTCTCTCCCA 
CAGCTGGTGG AGATGATTGC AGACCACCAG ATCGAGACAG GCCTCTTGAA GCCCGACCTC 
AAGGATAAGG TCACCTATAC GCTGCTGCGG AAACACCGAC ATCAAACCAA GAAATCCAAC 
CTTCGGTCCC TGGCTGACAT TGGGAAGACA GTCTCCAGTG CAAGTAGGAT GTTCAGCAAC 
CCTGATAACG GTAGCCCAGC TATGACCCAC AGGAATCTGA CATCCTCCAG TCTCAATGAC 
ATTTCTGATA AACCAGAGAA GGACCAGCTG AAGAATAAAT TCATGAAAAA ACTGCCCCGT 
GATGCAGAAG CTTCCAACGT GCTCGTTGGG GAGGTTGACT TCTTGGACAC TCCCTTCATT 
GCCTTTGTTC GTCTACAGCA GGCTGTCATG CTGGGTGCCC TGACTGAGGT GCCTGTGCCC 
ACAAGGTTCT TGTTCATTCT CTTAGGTCCA AAGGGGAAAG CCAAGTCCTA CCATGAGATC 
GGAAGAGCCA TCGCCACCTT GATGTCTGAC GAGGTGTTCC ACGATATCGC TTACAAAGCG 
AAAGATAGAC ACGACCTGAT TGCTGGCATT GACGAGTTCT TAGATGAAGT CATTGTCCTT 
CCACCTGGGG AATGGGACCC GGCAATCAGA ATAGAACCTC CAAAGAGCCT CCCATCATCT 
GACAAAAGGA AGAATATGTA CTCAGGTGGA GAGAACGTTC AGATGAATGG GGACACACCT 
CATGATGGAG GCCACGGAGG AGGAGGCCAC GGTGACTGTG AAGAACTACA GAGAACTGGC 
CGGTTCTGTG GTGGATTAAT TAAGGACATA AAGAGGAAAG CACCATTTTT TGCCAGTGAC 
TTTTATGACG CTTTAAATAT TCAGGCTCTC TCGGCAATTC TCTTCATCTA TCTGGCGACG 
GTAACCAACG CCATCACTTT CGGAGGCCTG CTCGGGGATG CCACTGACAA CATGCAGGGT 
GTGTTGGAGA GTTTCCTGGG CACTGCTGTC TCTGGCGCCA TCTTTTGCCT TTTTGCGGGT 
CAACCGCTCA CCATCTTGAG TAGCACGGGA CCTGTTCTGG TGTTTGAGAG GCTTCTTTTC 
AACTTCAGCA AGGACCATAG TTTTGACTAC TTGGAGTTTC GTCTTTGGAT TGGCCTGTGG 
TCAGCCTTCA TGTGTCTCAT CCTGGTGGCC ACTGATGCCA GCTTCCTGGT TCAGTACTTC 
ACCCGTTTCA CGGAAGAAGG ATTCTCCTCT CTGATCAGCT TCATCTTCAT CTATGATGCT 
TTCAAGAAGA TGATCAAGCT GGCAGATTAC TATCCCATCA ACTCTGACTT CAGAGTGGGC 
TACAACACTC ACTTCTCCTG TGCTTGCCTG CCACCTGACC CAGTTAATCT CTCAGTATCT 
AATGATACCA CACTAGCCCC AGAGGACCTG CCAACCGTTT CTTCTACTGA CATGTACCAC 
AATGCTACCT TTGACTGGGC CTACTTGTCA AAGAAAGAGT GTGTGAAATT TGGAGGAAAG 
CTCGTGGGAA ACAACTGTGA CTTCGTGCCT GACATCACAC TCATGTCCTT CATCCTCTTC 
CTGGGCACTT ACACCTCTTC TATGGCCATG AAGAAATTCA AAACCAGCCG CTATTTTCCA 
ACCACAGCAA GAAAACTGAT CAGCGATTTT GCCATCATCC TGTCCATTCT CATATTCTGT 
GTAATAGACG CCCTCGTCGG TGTGGATACT CCGAAGCTAA TTGTGCCAAG TGAGTTCAAG 
CCAACAAGCC CTCACCGGGG TTGGTTTGTC CCACCCTTTG GAGGAAACCC CTGGTGGGTG 
TGCCTTGCTG CTGCTATCCC GGCTTTGCTA GTCACCATCC TGATTTTCAT GGACCAGCAA 
ATCACGGCCG TGATTGTGAA CAGGAAAGAA CATAAGCTCA AGAAGGGAGC TGGCTATCAC 
CTGGACCTGT TTTGGGTCGC CATCCTCATG GTGGTGTGCT CCTTCATGGC TCTTCCCTGG 
TATGTGGCTG CTACTGTCAT CTCCATCGCT CACATCGACA GTCTGAAGAT GGAGACAGAG 
ACTTCTGCGC CTGGAGAACA ACCAAAGTTT CTGGGAGTGA GAGAACAACG AGTCACTGGA 
ACCCTTGTGT TTATTCTGAC TGGCCTGTCA GTCTTTATGG CTCCCATCTT GAAGTTTATC 
CCCATGCCTG TTCTGTATGG CGTGTTCTTG TATATGGGGG TGGCCTCACT GAACGGTGTG 
CAGTTCATGG ACCGTCTTAA GCTGCTTCTC ATGCCCCTGA AGCATCAACC TGACTTCATC 
TACCTGCGCC ACGTCCCCCT GCGCCGAGTC CACCTGTTCA CCTCCCTGCA GGTGTTGTGC 
CTGGCTCTGC TCTGGATCCT CAAGTCCACA GTGGCTGCGA TTATTTTTCC AGTTATGATC 
CTGGCCCTGG TAGCTGTCAG AAAAGGTATG GACTACCTCT TCTCCCAGCA CGACCTCAGC 
TTCCTTGATG ATGTCATTCC AGAGAAGGAC AAGAAAAAGA AGGAGGATGA GAAGAAGAAG 
AAAAAGAAGA AAGGAAGTCT GGATAGCGAC AACGACGATT CTGACTGCCC ATACTCAGAA 
AAGGTCCCCA GTATTAAAAT TCCAATGGAC ATCACGGAAC AGCAACCTTT CCTAAGTGAT 
AACAAACCCT TGGACAGAGA AAGATCCTCA ACATTCCTTG AACGCCACAC ATCATGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_445876.1
CDS	54..3293
Translation	MEDEAVLDRGASFLKHVCDEEEVEGHHTIYIGVHVPKSYRRRRRHKRKAGHKEKKEKERISENYSDKSDVENADESSSSILKPLISPAAERIRFILGEEDDSPAPPQLFTELDELLAVDGQEMEWKETARWIKFEEKVEQGGERWSKPHVATLSLHSLFELRTCMEKGSIMLDREASSLPQLVEMIADHQIETGLLKPDLKDKVTYTLLRKHRHQTKKSNLRSLADIGKTVSSASRMFSNPDNGSPAMTHRNLTSSSLNDISDKPEKDQLKNKFMKKLPRDAEASNVLVGEVDFLDTPFIAFVRLQQAVMLGALTEVPVPTRFLFILLGPKGKAKSYHEIGRAIATLMSDEVFHDIAYKAKDRHDLIAGIDEFLDEVIVLPPGEWDPAIRIEPPKSLPSSDKRKNMYSGGENVQMNGDTPHDGGHGGGGHGDCEELQRTGRFCGGLIKDIKRKAPFFASDFYDALNIQALSAILFIYLATVTNAITFGGLLGDATDNMQGVLESFLGTAVSGAIFCLFAGQPLTILSSTGPVLVFERLLFNFSKDHSFDYLEFRLWIGLWSAFMCLILVATDASFLVQYFTRFTEEGFSSLISFIFIYDAFKKMIKLADYYPINSDFRVGYNTHFSCACLPPDPVNLSVSNDTTLAPEDLPTVSSTDMYHNATFDWAYLSKKECVKFGGKLVGNNCDFVPDITLMSFILFLGTYTSSMAMKKFKTSRYFPTTARKLISDFAIILSILIFCVIDALVGVDTPKLIVPSEFKPTSPHRGWFVPPFGGNPWWVCLAAAIPALLVTILIFMDQQITAVIVNRKEHKLKKGAGYHLDLFWVAILMVVCSFMALPWYVAATVISIAHIDSLKMETETSAPGEQPKFLGVREQRVTGTLVFILTGLSVFMAPILKFIPMPVLYGVFLYMGVASLNGVQFMDRLKLLLMPLKHQPDFIYLRHVPLRRVHLFTSLQVLCLALLWILKSTVAAIIFPVMILALVAVRKGMDYLFSQHDLSFLDDVIPEKDKKKKEDEKKKKKKKGSLDSDNDDSDCPYSEKVPSIKIPMDITEQQPFLSDNKPLDRERSSTFLERHTSC

Target ORF information:

RefSeq Version	NM_053424.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus solute carrier family 4 member 4 (Slc4a4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_053424.1

ORF Insert Sequence:

ATGGAGGATG AAGCTGTCCT GGACAGAGGG GCTTCCTTCC TTAAACATGT GTGTGACGAA 
GAAGAAGTAG AAGGTCACCA CACGATCTAC ATTGGGGTCC ATGTGCCCAA GAGCTACCGG 
AGAAGGAGAC GCCATAAGAG AAAGGCTGGG CACAAGGAAA AGAAGGAGAA GGAAAGGATC 
TCTGAGAACT ATTCCGACAA ATCAGATGTG GAGAATGCGG ACGAATCCAG CAGCAGCATC 
CTCAAGCCGC TCATCTCCCC GGCCGCAGAA CGTATCCGAT TCATCTTGGG AGAGGAAGAT 
GACAGCCCAG CACCTCCTCA GCTCTTCACG GAACTCGATG AGCTGCTGGC TGTGGATGGA 
CAGGAGATGG AATGGAAGGA GACAGCCAGG TGGATTAAGT TTGAAGAGAA AGTGGAACAG 
GGTGGGGAGC GATGGAGCAA ACCCCATGTG GCCACCTTGT CCCTGCACAG CTTGTTTGAG 
CTGAGGACAT GTATGGAGAA AGGATCCATC ATGCTTGACC GGGAGGCCTC CTCTCTCCCA 
CAGCTGGTGG AGATGATTGC AGACCACCAG ATCGAGACAG GCCTCTTGAA GCCCGACCTC 
AAGGATAAGG TCACCTATAC GCTGCTGCGG AAACACCGAC ATCAAACCAA GAAATCCAAC 
CTTCGGTCCC TGGCTGACAT TGGGAAGACA GTCTCCAGTG CAAGTAGGAT GTTCAGCAAC 
CCTGATAACG GTAGCCCAGC TATGACCCAC AGGAATCTGA CATCCTCCAG TCTCAATGAC 
ATTTCTGATA AACCAGAGAA GGACCAGCTG AAGAATAAAT TCATGAAAAA ACTGCCCCGT 
GATGCAGAAG CTTCCAACGT GCTCGTTGGG GAGGTTGACT TCTTGGACAC TCCCTTCATT 
GCCTTTGTTC GTCTACAGCA GGCTGTCATG CTGGGTGCCC TGACTGAGGT GCCTGTGCCC 
ACAAGGTTCT TGTTCATTCT CTTAGGTCCA AAGGGGAAAG CCAAGTCCTA CCATGAGATC 
GGAAGAGCCA TCGCCACCTT GATGTCTGAC GAGGTGTTCC ACGATATCGC TTACAAAGCG 
AAAGATAGAC ACGACCTGAT TGCTGGCATT GACGAGTTCT TAGATGAAGT CATTGTCCTT 
CCACCTGGGG AATGGGACCC GGCAATCAGA ATAGAACCTC CAAAGAGCCT CCCATCATCT 
GACAAAAGGA AGAATATGTA CTCAGGTGGA GAGAACGTTC AGATGAATGG GGACACACCT 
CATGATGGAG GCCACGGAGG AGGAGGCCAC GGTGACTGTG AAGAACTACA GAGAACTGGC 
CGGTTCTGTG GTGGATTAAT TAAGGACATA AAGAGGAAAG CACCATTTTT TGCCAGTGAC 
TTTTATGACG CTTTAAATAT TCAGGCTCTC TCGGCAATTC TCTTCATCTA TCTGGCGACG 
GTAACCAACG CCATCACTTT CGGAGGCCTG CTCGGGGATG CCACTGACAA CATGCAGGGT 
GTGTTGGAGA GTTTCCTGGG CACTGCTGTC TCTGGCGCCA TCTTTTGCCT TTTTGCGGGT 
CAACCGCTCA CCATCTTGAG TAGCACGGGA CCTGTTCTGG TGTTTGAGAG GCTTCTTTTC 
AACTTCAGCA AGGACCATAG TTTTGACTAC TTGGAGTTTC GTCTTTGGAT TGGCCTGTGG 
TCAGCCTTCA TGTGTCTCAT CCTGGTGGCC ACTGATGCCA GCTTCCTGGT TCAGTACTTC 
ACCCGTTTCA CGGAAGAAGG ATTCTCCTCT CTGATCAGCT TCATCTTCAT CTATGATGCT 
TTCAAGAAGA TGATCAAGCT GGCAGATTAC TATCCCATCA ACTCTGACTT CAGAGTGGGC 
TACAACACTC ACTTCTCCTG TGCTTGCCTG CCACCTGACC CAGTTAATCT CTCAGTATCT 
AATGATACCA CACTAGCCCC AGAGGACCTG CCAACCGTTT CTTCTACTGA CATGTACCAC 
AATGCTACCT TTGACTGGGC CTACTTGTCA AAGAAAGAGT GTGTGAAATT TGGAGGAAAG 
CTCGTGGGAA ACAACTGTGA CTTCGTGCCT GACATCACAC TCATGTCCTT CATCCTCTTC 
CTGGGCACTT ACACCTCTTC TATGGCCATG AAGAAATTCA AAACCAGCCG CTATTTTCCA 
ACCACAGCAA GAAAACTGAT CAGCGATTTT GCCATCATCC TGTCCATTCT CATATTCTGT 
GTAATAGACG CCCTCGTCGG TGTGGATACT CCGAAGCTAA TTGTGCCAAG TGAGTTCAAG 
CCAACAAGCC CTCACCGGGG TTGGTTTGTC CCACCCTTTG GAGGAAACCC CTGGTGGGTG 
TGCCTTGCTG CTGCTATCCC GGCTTTGCTA GTCACCATCC TGATTTTCAT GGACCAGCAA 
ATCACGGCCG TGATTGTGAA CAGGAAAGAA CATAAGCTCA AGAAGGGAGC TGGCTATCAC 
CTGGACCTGT TTTGGGTCGC CATCCTCATG GTGGTGTGCT CCTTCATGGC TCTTCCCTGG 
TATGTGGCTG CTACTGTCAT CTCCATCGCT CACATCGACA GTCTGAAGAT GGAGACAGAG 
ACTTCTGCGC CTGGAGAACA ACCAAAGTTT CTGGGAGTGA GAGAACAACG AGTCACTGGA 
ACCCTTGTGT TTATTCTGAC TGGCCTGTCA GTCTTTATGG CTCCCATCTT GAAGTTTATC 
CCCATGCCTG TTCTGTATGG CGTGTTCTTG TATATGGGGG TGGCCTCACT GAACGGTGTG 
CAGTTCATGG ACCGTCTTAA GCTGCTTCTC ATGCCCCTGA AGCATCAACC TGACTTCATC 
TACCTGCGCC ACGTCCCCCT GCGCCGAGTC CACCTGTTCA CCTCCCTGCA GGTGTTGTGC 
CTGGCTCTGC TCTGGATCCT CAAGTCCACA GTGGCTGCGA TTATTTTTCC AGTTATGATC 
CTGGCCCTGG TAGCTGTCAG AAAAGGTATG GACTACCTCT TCTCCCAGCA CGACCTCAGC 
TTCCTTGATG ATGTCATTCC AGAGAAGGAC AAGAAAAAGA AGGAGGATGA GAAGAAGAAG 
AAAAAGAAGA AAGGAAGTCT GGATAGCGAC AACGACGATT CTGACTGCCC ATACTCAGAA 
AAGGTCCCCA GTATTAAAAT TCCAATGGAC ATCACGGAAC AGCAACCTTT CCTAAGTGAT 
AACAAACCCT TGGACAGAGA AAGATCCTCA ACATTCCTTG AACGCCACAC ATCATGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Slc4a4 ( XM_006250791.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006250853.1 Rattus norvegicus solute carrier family 4 member 4 (Slc4a4), transcript variant X2, mRNA.

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CloneID	ORa35943
Clone ID Related Accession (Same CDS sequence)	XM_006250791.3
Accession Version	XM_006250791.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3285bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	electrogenic sodium bicarbonate cotransporter 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005113.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006250791.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGATG AAGCTGTCCT GGACAGAGGG GCTTCCTTCC TTAAACATGT GTGTGACGAA 
GAAGAAGTAG AAGGTCACCA CACGATCTAC ATTGGGGTCC ATGTGCCCAA GAGCTACCGG 
AGAAGGAGAC GCCATAAGAG AAAGGCTGGG CACAAGGAAA AGAAGGAGAA GGAAAGGATC 
TCTGAGAACT ATTCCGACAA ATCAGATGTG GAGAATGCGG ACGAATCCAG CAGCAGCATC 
CTCAAGCCGC TCATCTCCCC GGCCGCAGAA CGTATCCGAT TCATCTTGGG AGAGGAAGAT 
GACAGCCCAG CACCTCCTCA GCTCTTCACG GAACTCGATG AGCTGCTGGC TGTGGATGGA 
CAGGAGATGG AATGGAAGGA GACAGCCAGG TGGATTAAGT TTGAAGAGAA AGTGGAACAG 
GGTGGGGAGC GATGGAGCAA ACCCCATGTG GCCACCTTGT CCCTGCACAG CTTGTTTGAG 
CTGAGGACAT GTATGGAGAA AGGATCCATC ATGCTTGACC GGGAGGCCTC CTCTCTCCCA 
CAGCTGGTGG AGATGATTGC AGACCACCAG ATCGAGACAG GCCTCTTGAA GCCCGACCTC 
AAGGATAAGG TCACCTATAC GCTGCTGCGG AAACACCGAC ATCAAACCAA GAAATCCAAC 
CTTCGGTCCC TGGCTGACAT TGGGAAGACA GTCTCCAGTG CAAGTAGGAT GTTCAGCAAC 
CCTGATAACG GTAGCCCAGC TATGACCCAC AGGAATCTGA CATCCTCCAG TCTCAATGAC 
ATTTCTGATA AACCAGAGAA GGACCAGCTG AAGAATAAAT TCATGAAAAA ACTGCCCCGT 
GATGCAGAAG CTTCCAACGT GCTCGTTGGG GAGGTTGACT TCTTGGACAC TCCCTTCATT 
GCCTTTGTTC GTCTACAGCA GGCTGTCATG CTGGGTGCCC TGACTGAGGT GCCTGTGCCC 
ACAAGGTTCT TGTTCATTCT CTTAGGTCCA AAGGGGAAAG CCAAGTCCTA CCATGAGATC 
GGAAGAGCCA TCGCCACCTT GATGTCTGAC GAGGTGTTCC ACGATATCGC TTACAAAGCG 
AAAGATAGAC ACGACCTGAT TGCTGGCATT GACGAGTTCT TAGATGAAGT CATTGTCCTT 
CCACCTGGGG AATGGGACCC GGCAATCAGA ATAGAACCTC CAAAGAGCCT CCCATCATCT 
GACAAAAGGA AGAATATGTA CTCAGGTGGA GAGAACGTTC AGATGAATGG GGACACACCT 
CATGATGGAG GCCACGGAGG AGGAGGCCAC GGTGACTGTG AAGAACTACA GAGAACTGGC 
CGGTTCTGTG GTGGATTAAT TAAGGACATA AAGAGGAAAG CACCATTTTT TGCCAGTGAC 
TTTTATGACG CTTTAAATAT TCAGGCTCTC TCGGCAATTC TCTTCATCTA TCTGGCGACG 
GTAACCAACG CCATCACTTT CGGAGGCCTG CTCGGGGATG CCACTGACAA CATGCAGGGT 
GTGTTGGAGA GTTTCCTGGG CACTGCTGTC TCTGGCGCCA TCTTTTGCCT TTTTGCGGGT 
CAACCGCTCA CCATCTTGAG TAGCACGGGA CCTGTTCTGG TGTTTGAGAG GCTTCTTTTC 
AACTTCAGCA AGGACCATAG TTTTGACTAC TTGGAGTTTC GTCTTTGGAT TGGCCTGTGG 
TCAGCCTTCA TGTGTCTCAT CCTGGTGGCC ACTGATGCCA GCTTCCTGGT TCAGTACTTC 
ACCCGTTTCA CGGAAGAAGG ATTCTCCTCT CTGATCAGCT TCATCTTCAT CTATGATGCT 
TTCAAGAAGA TGATCAAGCT GGCAGATTAC TATCCCATCA ACTCTGACTT CAGAGTGGGC 
TACAACACTC ACTTCTCCTG TGCTTGCCTG CCACCTGACC CAGTTAATCT CTCAGTATCT 
AATGATACCA CACTAGCCCC AGAGGACCTG CCAACCGTTT CTTCTACTGA CATGTACCAC 
AATGCTACCT TTGACTGGGC CTACTTGTCA AAGAAAGAGT GTGTGAAATT TGGAGGAAAG 
CTCGTGGGAA ACAACTGTGA CTTCGTGCCT GACATCACAC TCATGTCCTT CATCCTCTTC 
CTGGGCACTT ACACCTCTTC TATGGCCATG AAGAAATTCA AAACCAGCCG CTATTTTCCA 
ACCACAGCAA GAAAACTGAT CAGCGATTTT GCCATCATCC TGTCCATTCT CATATTCTGT 
GTAATAGACG CCCTCGTCGG TGTGGATACT CCGAAGCTAA TTGTGCCAAG TGAGTTCAAG 
CCAACAAGCC CTCACCGGGG TTGGTTTGTC CCACCCTTTG GAGGAAACCC CTGGTGGGTG 
TGCCTTGCTG CTGCTATCCC GGCTTTGCTA GTCACCATCC TGATTTTCAT GGACCAGCAA 
ATCACGGCCG TGATTGTGAA CAGGAAAGAA CATAAGCTCA AGAAGGGAGC TGGCTATCAC 
CTGGACCTGT TTTGGGTCGC CATCCTCATG GTGGTGTGCT CCTTCATGGC TCTTCCCTGG 
TATGTGGCTG CTACTGTCAT CTCCATCGCT CACATCGACA GTCTGAAGAT GGAGACAGAG 
ACTTCTGCGC CTGGAGAACA ACCAAAGTTT CTGGGAGTGA GAGAACAACG AGTCACTGGA 
ACCCTTGTGT TTATTCTGAC TGGCCTGTCA GTCTTTATGG CTCCCATCTT GAAGTTTATC 
CCCATGCCTG TTCTGTATGG CGTGTTCTTG TATATGGGGG TGGCCTCACT GAACGGTGTG 
CAGTTCATGG ACCGTCTTAA GCTGCTTCTC ATGCCCCTGA AGCATCAACC TGACTTCATC 
TACCTGCGCC ACGTCCCCCT GCGCCGAGTC CACCTGTTCA CCTCCCTGCA GGTGTTGTGC 
CTGGCTCTGC TCTGGATCCT CAAGTCCACA GTGGCTGCGA TTATTTTTCC AGTTATGATC 
CTGGCCCTGG TAGCTGTCAG AAAAGGTATG GACTACCTCT TCTCCCAGCA CGACCTCAGC 
TTCCTTGATG ATGTCATTCC AGAGAAGGAC AAGAAAAAGA AGGAGGATGA GAAGAAGAAG 
AAAAAGAAGA AAGGAAGTCT GGATAGCGAC AACGACGATG AGAAAGATCC TCAACATTCC 
TTGAACGCCA CACATCATGC TGATAAAATT CCTTTCCTTG AGTCACTGGG TTTACCCAGT 
CCTCCAAGAT CTCCAGTGAA AGTCGTGCCT CAAATTAGAA TAGAACTTGA GTCTGAAGAC 
AACGATTATC TTTGGAGGAA CAAGGGAACA GAAACCACGT TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006250853.1
CDS	508..3792
Translation	MEDEAVLDRGASFLKHVCDEEEVEGHHTIYIGVHVPKSYRRRRRHKRKAGHKEKKEKERISENYSDKSDVENADESSSSILKPLISPAAERIRFILGEEDDSPAPPQLFTELDELLAVDGQEMEWKETARWIKFEEKVEQGGERWSKPHVATLSLHSLFELRTCMEKGSIMLDREASSLPQLVEMIADHQIETGLLKPDLKDKVTYTLLRKHRHQTKKSNLRSLADIGKTVSSASRMFSNPDNGSPAMTHRNLTSSSLNDISDKPEKDQLKNKFMKKLPRDAEASNVLVGEVDFLDTPFIAFVRLQQAVMLGALTEVPVPTRFLFILLGPKGKAKSYHEIGRAIATLMSDEVFHDIAYKAKDRHDLIAGIDEFLDEVIVLPPGEWDPAIRIEPPKSLPSSDKRKNMYSGGENVQMNGDTPHDGGHGGGGHGDCEELQRTGRFCGGLIKDIKRKAPFFASDFYDALNIQALSAILFIYLATVTNAITFGGLLGDATDNMQGVLESFLGTAVSGAIFCLFAGQPLTILSSTGPVLVFERLLFNFSKDHSFDYLEFRLWIGLWSAFMCLILVATDASFLVQYFTRFTEEGFSSLISFIFIYDAFKKMIKLADYYPINSDFRVGYNTHFSCACLPPDPVNLSVSNDTTLAPEDLPTVSSTDMYHNATFDWAYLSKKECVKFGGKLVGNNCDFVPDITLMSFILFLGTYTSSMAMKKFKTSRYFPTTARKLISDFAIILSILIFCVIDALVGVDTPKLIVPSEFKPTSPHRGWFVPPFGGNPWWVCLAAAIPALLVTILIFMDQQITAVIVNRKEHKLKKGAGYHLDLFWVAILMVVCSFMALPWYVAATVISIAHIDSLKMETETSAPGEQPKFLGVREQRVTGTLVFILTGLSVFMAPILKFIPMPVLYGVFLYMGVASLNGVQFMDRLKLLLMPLKHQPDFIYLRHVPLRRVHLFTSLQVLCLALLWILKSTVAAIIFPVMILALVAVRKGMDYLFSQHDLSFLDDVIPEKDKKKKEDEKKKKKKKGSLDSDNDDEKDPQHSLNATHHADKIPFLESLGLPSPPRSPVKVVPQIRIELESEDNDYLWRNKGTETTL

Target ORF information:

RefSeq Version	XM_006250791.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus solute carrier family 4 member 4 (Slc4a4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006250791.3

ORF Insert Sequence:

ATGGAGGATG AAGCTGTCCT GGACAGAGGG GCTTCCTTCC TTAAACATGT GTGTGACGAA 
GAAGAAGTAG AAGGTCACCA CACGATCTAC ATTGGGGTCC ATGTGCCCAA GAGCTACCGG 
AGAAGGAGAC GCCATAAGAG AAAGGCTGGG CACAAGGAAA AGAAGGAGAA GGAAAGGATC 
TCTGAGAACT ATTCCGACAA ATCAGATGTG GAGAATGCGG ACGAATCCAG CAGCAGCATC 
CTCAAGCCGC TCATCTCCCC GGCCGCAGAA CGTATCCGAT TCATCTTGGG AGAGGAAGAT 
GACAGCCCAG CACCTCCTCA GCTCTTCACG GAACTCGATG AGCTGCTGGC TGTGGATGGA 
CAGGAGATGG AATGGAAGGA GACAGCCAGG TGGATTAAGT TTGAAGAGAA AGTGGAACAG 
GGTGGGGAGC GATGGAGCAA ACCCCATGTG GCCACCTTGT CCCTGCACAG CTTGTTTGAG 
CTGAGGACAT GTATGGAGAA AGGATCCATC ATGCTTGACC GGGAGGCCTC CTCTCTCCCA 
CAGCTGGTGG AGATGATTGC AGACCACCAG ATCGAGACAG GCCTCTTGAA GCCCGACCTC 
AAGGATAAGG TCACCTATAC GCTGCTGCGG AAACACCGAC ATCAAACCAA GAAATCCAAC 
CTTCGGTCCC TGGCTGACAT TGGGAAGACA GTCTCCAGTG CAAGTAGGAT GTTCAGCAAC 
CCTGATAACG GTAGCCCAGC TATGACCCAC AGGAATCTGA CATCCTCCAG TCTCAATGAC 
ATTTCTGATA AACCAGAGAA GGACCAGCTG AAGAATAAAT TCATGAAAAA ACTGCCCCGT 
GATGCAGAAG CTTCCAACGT GCTCGTTGGG GAGGTTGACT TCTTGGACAC TCCCTTCATT 
GCCTTTGTTC GTCTACAGCA GGCTGTCATG CTGGGTGCCC TGACTGAGGT GCCTGTGCCC 
ACAAGGTTCT TGTTCATTCT CTTAGGTCCA AAGGGGAAAG CCAAGTCCTA CCATGAGATC 
GGAAGAGCCA TCGCCACCTT GATGTCTGAC GAGGTGTTCC ACGATATCGC TTACAAAGCG 
AAAGATAGAC ACGACCTGAT TGCTGGCATT GACGAGTTCT TAGATGAAGT CATTGTCCTT 
CCACCTGGGG AATGGGACCC GGCAATCAGA ATAGAACCTC CAAAGAGCCT CCCATCATCT 
GACAAAAGGA AGAATATGTA CTCAGGTGGA GAGAACGTTC AGATGAATGG GGACACACCT 
CATGATGGAG GCCACGGAGG AGGAGGCCAC GGTGACTGTG AAGAACTACA GAGAACTGGC 
CGGTTCTGTG GTGGATTAAT TAAGGACATA AAGAGGAAAG CACCATTTTT TGCCAGTGAC 
TTTTATGACG CTTTAAATAT TCAGGCTCTC TCGGCAATTC TCTTCATCTA TCTGGCGACG 
GTAACCAACG CCATCACTTT CGGAGGCCTG CTCGGGGATG CCACTGACAA CATGCAGGGT 
GTGTTGGAGA GTTTCCTGGG CACTGCTGTC TCTGGCGCCA TCTTTTGCCT TTTTGCGGGT 
CAACCGCTCA CCATCTTGAG TAGCACGGGA CCTGTTCTGG TGTTTGAGAG GCTTCTTTTC 
AACTTCAGCA AGGACCATAG TTTTGACTAC TTGGAGTTTC GTCTTTGGAT TGGCCTGTGG 
TCAGCCTTCA TGTGTCTCAT CCTGGTGGCC ACTGATGCCA GCTTCCTGGT TCAGTACTTC 
ACCCGTTTCA CGGAAGAAGG ATTCTCCTCT CTGATCAGCT TCATCTTCAT CTATGATGCT 
TTCAAGAAGA TGATCAAGCT GGCAGATTAC TATCCCATCA ACTCTGACTT CAGAGTGGGC 
TACAACACTC ACTTCTCCTG TGCTTGCCTG CCACCTGACC CAGTTAATCT CTCAGTATCT 
AATGATACCA CACTAGCCCC AGAGGACCTG CCAACCGTTT CTTCTACTGA CATGTACCAC 
AATGCTACCT TTGACTGGGC CTACTTGTCA AAGAAAGAGT GTGTGAAATT TGGAGGAAAG 
CTCGTGGGAA ACAACTGTGA CTTCGTGCCT GACATCACAC TCATGTCCTT CATCCTCTTC 
CTGGGCACTT ACACCTCTTC TATGGCCATG AAGAAATTCA AAACCAGCCG CTATTTTCCA 
ACCACAGCAA GAAAACTGAT CAGCGATTTT GCCATCATCC TGTCCATTCT CATATTCTGT 
GTAATAGACG CCCTCGTCGG TGTGGATACT CCGAAGCTAA TTGTGCCAAG TGAGTTCAAG 
CCAACAAGCC CTCACCGGGG TTGGTTTGTC CCACCCTTTG GAGGAAACCC CTGGTGGGTG 
TGCCTTGCTG CTGCTATCCC GGCTTTGCTA GTCACCATCC TGATTTTCAT GGACCAGCAA 
ATCACGGCCG TGATTGTGAA CAGGAAAGAA CATAAGCTCA AGAAGGGAGC TGGCTATCAC 
CTGGACCTGT TTTGGGTCGC CATCCTCATG GTGGTGTGCT CCTTCATGGC TCTTCCCTGG 
TATGTGGCTG CTACTGTCAT CTCCATCGCT CACATCGACA GTCTGAAGAT GGAGACAGAG 
ACTTCTGCGC CTGGAGAACA ACCAAAGTTT CTGGGAGTGA GAGAACAACG AGTCACTGGA 
ACCCTTGTGT TTATTCTGAC TGGCCTGTCA GTCTTTATGG CTCCCATCTT GAAGTTTATC 
CCCATGCCTG TTCTGTATGG CGTGTTCTTG TATATGGGGG TGGCCTCACT GAACGGTGTG 
CAGTTCATGG ACCGTCTTAA GCTGCTTCTC ATGCCCCTGA AGCATCAACC TGACTTCATC 
TACCTGCGCC ACGTCCCCCT GCGCCGAGTC CACCTGTTCA CCTCCCTGCA GGTGTTGTGC 
CTGGCTCTGC TCTGGATCCT CAAGTCCACA GTGGCTGCGA TTATTTTTCC AGTTATGATC 
CTGGCCCTGG TAGCTGTCAG AAAAGGTATG GACTACCTCT TCTCCCAGCA CGACCTCAGC 
TTCCTTGATG ATGTCATTCC AGAGAAGGAC AAGAAAAAGA AGGAGGATGA GAAGAAGAAG 
AAAAAGAAGA AAGGAAGTCT GGATAGCGAC AACGACGATG AGAAAGATCC TCAACATTCC 
TTGAACGCCA CACATCATGC TGATAAAATT CCTTTCCTTG AGTCACTGGG TTTACCCAGT 
CCTCCAAGAT CTCCAGTGAA AGTCGTGCCT CAAATTAGAA TAGAACTTGA GTCTGAAGAC 
AACGATTATC TTTGGAGGAA CAAGGGAACA GAAACCACGT TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Modulation of oxidative and glycolytic skeletal muscle fibers Na+/H+ exchanger1 (NHE1) and Na+/HCO3- co-transporter1 (NBC1) genes and proteins expression in type 2 diabetic rat (Streptozotocin + high fat diet) following long term endurance training.
Cellular and molecular biology (Noisy-le-Grand, France)63(5)11-18(2017 May)
Monazzami A,Rajabi H,Ghrakhanlou R,Yari K,Rahimi Z

Na
World journal of gastroenterology22(43)9525-9533(2016 Nov)
Cao LH,Xia CC,Shi ZC,Wang N,Gu ZH,Yu LZ,Wan Q,De W

The cardiac electrogenic sodium/bicarbonate cotransporter (NBCe1) is activated by aldosterone through the G protein-coupled receptor 30 (GPR 30).
Channels (Austin, Tex.)10(5)428-434(2016 Sep)
Orlowski A,De Giusti VC,Ciancio MC,Espejo MS,Aiello EA

Aldosterone stimulates the cardiac sodium/bicarbonate cotransporter via activation of the g protein-coupled receptor gpr30.
Journal of molecular and cellular cardiology89(Pt B)260-7(2015 Dec)
De Giusti VC,Orlowski A,Ciancio MC,Espejo MS,Gonano LA,Caldiz CI,Vila Petroff MG,Villa-Abrille MC,Aiello EA

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Slc4a4 cDNA ORF clone, Rattus norvegicus(Norway rat)

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