Pomt1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pomt1
Entrez Gene ID	84430
Full Name	protein-O-mannosyltransferase 1
General protein information	Preferred Names protein-O-mannosyltransferase 1 Names protein O-mannosyl-transferase 1 dolichyl-phosphate-mannose--protein mannosyltransferase 1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 3 Map Location: 3p12
Summary	human homolog is mutated in patients with Walker-Warburg syndrome, a neuronal migration disorder [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_008761776.1	XP_008759998.1	protein O-mannosyl-transferase 1 isoform X1
NM_053406.2	NP_445858.1	protein O-mannosyl-transferase 1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	POMT1	NP_001073799.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010784	XP_318526.4
Magnaporthe oryzae (rice blast fungus)	MGG_04427	XP_003713520.1
Neurospora crassa	NCU09332	XP_963926.2
Mus musculus (house mouse)	Pomt1	NP_660127.1
Gallus gallus (chicken)	POMT1	NP_001025856.1
Homo sapiens (human)	POMT1	NP_009102.3
Pan troglodytes (chimpanzee)	POMT1	NP_001233574.1
	POMT1	XP_002799997.1
Rattus norvegicus (Norway rat)	Pomt1	NP_445858.1
Danio rerio (zebrafish)	pomt1	NP_001041532.2
Saccharomyces cerevisiae (baker's yeast)	PMT4	NP_012677.1
Eremothecium gossypii	AGOS_ABL085W	NP_982862.2
Drosophila melanogaster (fruit fly)	rt	NP_524025.2
Canis lupus familiaris (dog)	POMT1	XP_849854.1
Kluyveromyces lactis	KLLA0B03762g	XP_451701.1
Schizosaccharomyces pombe (fission yeast)	ogm4	NP_596807.1
Xenopus tropicalis (tropical clawed frog)	pomt1	NP_001006756.1

Gene Ontology
Bibliography

Related articles in PubMed

Mammalian
Larsen ISB, Narimatsu Y, Joshi HJ, Yang Z, Harrison OJ, Brasch J, Shapiro L, Honig B, Vakhrushev SY, Clausen H, Halim A
The Journal of biological chemistry292(27)11586-11598(2017 07)

Cardiomyopathy in patients with POMT1-related congenital and limb-girdle muscular dystrophy.
Bello L, Melacini P, Pezzani R, D'Amico A, Piva L, Leonardi E, Torella A, Soraru G, Palmieri A, Smaniotto G, Gavassini BF, Vianello A, Nigro V, Bertini E, Angelini C, Tosatto SC, Pegoraro E
European journal of human genetics : EJHG20(12)1234-9(2012 Dec)

Walker-Warburg Syndrome with POMT1 mutations can be associated with cleft lip and cleft palate.
Vajsar J, Baskin B, Swoboda K, Biggar DW, Schachter H, Ray PN
Neuromuscular disorders : NMD18(8)675-7(2008 Aug)

Molecular heterogeneity in fetal forms of type II lissencephaly.
Bouchet C, Gonzales M, Vuillaumier-Barrot S, Devisme L, Lebizec C, Alanio E, Bazin A, Bessi?res-Grattagliano B, Bigi N, Blanchet P, Bonneau D, Bonni?res M, Carles D, Delahaye S, Fallet-Bianco C, Figarella-Branger D, Gaillard D, Gasser B, Guimiot F, Joubert M, Laurent N, Liprandi A, Loget P, Marcorelles P, Martinovic J, Menez F, Patrier S, Pelluard-Nehm? F, Perez MJ, Rouleau-Dubois C, Triau S, Laquerri?re A, Encha-Razavi F, Seta N
Human mutation28(10)1020-7(2007 Oct)

Expression of the murine Pomt1 gene in both the developing brain and adult muscle tissues and its relationship with clinical aspects of Walker-Warburg syndrome.
Prados B, Pe?a A, Cotarelo RP, Valero MC, Cruces J
The American journal of pathology170(5)1659-68(2007 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Pomt1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pomt1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa43833	XM_008761776.1 Latest version!	Rattus norvegicus protein-O-mannosyltransferase 1 (Pomt1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
ORa09462	NM_053406.2 Latest version!	Rattus norvegicus protein-O-mannosyltransferase 1 (Pomt1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pomt1 ( XM_008761776.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008759998.1 Rattus norvegicus protein-O-mannosyltransferase 1 (Pomt1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa43833
Clone ID Related Accession (Same CDS sequence)	XM_008761776.1
Accession Version	XM_008761776.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1827bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	protein O-mannosyl-transferase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTACC AGATAGTGTT GGAGCTCCAC TTTTCCCACT GTACTGCCAT GGGAGCCGCC 
CTGCTGATGC TCATTGAGAA CGCCCTAATC ACTCAGTCCA GGCTCATGCT GTTGGAATCC 
ATACTGATAT TTTTTAACCT CTTGGCCGTG TTGTCCTATC TGAAGTTCTT CAACTCCCAA 
ACACACAGCC CTTTCTCAGT GCACTGGTGG CTGTGGCTAA TGCTGACCGG AGTCTCTTGT 
TCCTGTGCGG TTGGGATCAA GTACATGGGC ATTTTCACCT ACTTGCTCGT GCTCAGCATT 
GCAGCTGTGC ATGCCTGGCA CCTGATCGGA GACCAGACCT TGTCAAATAT CTGCGTGCTC 
AGTCACTTGC TCGCCAGAGC CGTAGCTCTG CTGGTCGTCC CGGTCTTCCT GTACTTACTG 
TTCTTCTATG TCCACCTGAT GTTGCTCTAC CGCTCTGGGC CCCATGACCA AATCATGTCC 
AGTGCCTTCC AAGCCAGCTT GGAGGGAGGG CTAGCCCGCA TCACCCAAGG CCAGCCCCTA 
GAGGTGGCCT TTGGTTCGCA GGTCACTCTG AAGAGCGTCT CCGGCAAACC CTTGCCCTGC 
TGGCTTCATT CGCACAAGAA CACCTATCCC ATGATATATG AGAATGGCCG TGGCAGCTCC 
CACCAGCAAC AGGTGACCTG TTATCCCTTC AAAGACATCA ATAACTGGTG GATCGTCAAG 
GACCCTGGAC GACACCAGCT GGTGGTAAAC AACCCCCCCA GGCCTGTGAG ACATGGAGAC 
ATTGTACAGC TCGTTCACGG CATGACCACC CGCCTGCTTA ACACGCATGA TGTCGCTGCC 
CCGCTGAGCC CCCATTCTCA AGAAGTCTCC TGCTACATTG ACTATAACAT CTCCATGCCT 
GCCCAGAACC TCTGGAAACT GGACATTGTA AACAGAGAGT CCAACCAGGA TACCTGGAAG 
ACTATCTTGT CAGAAGTGCG CTTTGTGCAT GTGAATACAT CTGCCATCTT GAAGCTGAGC 
GGGGCTCACC TCCCTGACTG GGGATTTCGG CAGTTGGAGG TGGTTGGGGA GAAGTTGTCC 
CTCGGCCCCC ACGAGAGCAT GGTATGGAAT GTGGAAGAGC ACCGCTATGG CAGAGGCCAT 
GAGCAGAAGG AGAGGGAGCT GGAGCTCCAC TCACCCACGC AGCATGATAT CAGCAGGAAC 
CTCAGCTTCA TGGCCAGATT CTCGGAGTTA CAGTGGAAGA TGCTGACGCT GAAGAATGAG 
GACTTAGAAC ACCAGTACAG CTCCACCCCG CTGGAGTGGC TCACGCTGGA CACCAACATT 
GCCTATTGGC TGCACCCCAG GACCAGTGCC CAGATCCACT TGCTTGGAAA CATCGTGATC 
TGGACTTCAG CCAGCCTCGC CACAGTGGCA TACACCCTAC TCTTCTTCTG GTACCTGCTC 
CGCCGTCGAA GGAACATCTG TGACCTCCCT GAGGATGCCT GGTCCCACTG GGTGCTGGCT 
GGAGCCCTGT GTATTGGCGG TTGGGCACTC AACTATCTGC CCTTCTTCCT GATGGAAAGG 
ATGCTCTTCC TCTACCACTA CTTGCCGGCC CTCACCTTCC AGATCCTGCT GCTCCCAATC 
GTCATGCAGC ACGCCAGCGA CCATCTGTGC AGGTCCCAGC TGCAGAGGAA TGTCTTCAGT 
GCCCTGGTGG TAGCATGGTA TTCCTCTGCG TGCCATGTGT CCAACATGTT ACGCCCACTG 
ACCTATGGGG ACACGTCACT CTCACCAGGC GAGCTCCGGG CCCTTCGCTG GAAAGACAGC 
TGGGACATTC TCATCCGAAA ATACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008759998.1
CDS	216..2042
Translation	MAYQIVLELHFSHCTAMGAALLMLIENALITQSRLMLLESILIFFNLLAVLSYLKFFNSQTHSPFSVHWWLWLMLTGVSCSCAVGIKYMGIFTYLLVLSIAAVHAWHLIGDQTLSNICVLSHLLARAVALLVVPVFLYLLFFYVHLMLLYRSGPHDQIMSSAFQASLEGGLARITQGQPLEVAFGSQVTLKSVSGKPLPCWLHSHKNTYPMIYENGRGSSHQQQVTCYPFKDINNWWIVKDPGRHQLVVNNPPRPVRHGDIVQLVHGMTTRLLNTHDVAAPLSPHSQEVSCYIDYNISMPAQNLWKLDIVNRESNQDTWKTILSEVRFVHVNTSAILKLSGAHLPDWGFRQLEVVGEKLSLGPHESMVWNVEEHRYGRGHEQKERELELHSPTQHDISRNLSFMARFSELQWKMLTLKNEDLEHQYSSTPLEWLTLDTNIAYWLHPRTSAQIHLLGNIVIWTSASLATVAYTLLFFWYLLRRRRNICDLPEDAWSHWVLAGALCIGGWALNYLPFFLMERMLFLYHYLPALTFQILLLPIVMQHASDHLCRSQLQRNVFSALVVAWYSSACHVSNMLRPLTYGDTSLSPGELRALRWKDSWDILIRKY

Target ORF information:

RefSeq Version	XM_008761776.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus protein-O-mannosyltransferase 1 (Pomt1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008761776.1

ORF Insert Sequence:

ATGGCCTACC AGATAGTGTT GGAGCTCCAC TTTTCCCACT GTACTGCCAT GGGAGCCGCC 
CTGCTGATGC TCATTGAGAA CGCCCTAATC ACTCAGTCCA GGCTCATGCT GTTGGAATCC 
ATACTGATAT TTTTTAACCT CTTGGCCGTG TTGTCCTATC TGAAGTTCTT CAACTCCCAA 
ACACACAGCC CTTTCTCAGT GCACTGGTGG CTGTGGCTAA TGCTGACCGG AGTCTCTTGT 
TCCTGTGCGG TTGGGATCAA GTACATGGGC ATTTTCACCT ACTTGCTCGT GCTCAGCATT 
GCAGCTGTGC ATGCCTGGCA CCTGATCGGA GACCAGACCT TGTCAAATAT CTGCGTGCTC 
AGTCACTTGC TCGCCAGAGC CGTAGCTCTG CTGGTCGTCC CGGTCTTCCT GTACTTACTG 
TTCTTCTATG TCCACCTGAT GTTGCTCTAC CGCTCTGGGC CCCATGACCA AATCATGTCC 
AGTGCCTTCC AAGCCAGCTT GGAGGGAGGG CTAGCCCGCA TCACCCAAGG CCAGCCCCTA 
GAGGTGGCCT TTGGTTCGCA GGTCACTCTG AAGAGCGTCT CCGGCAAACC CTTGCCCTGC 
TGGCTTCATT CGCACAAGAA CACCTATCCC ATGATATATG AGAATGGCCG TGGCAGCTCC 
CACCAGCAAC AGGTGACCTG TTATCCCTTC AAAGACATCA ATAACTGGTG GATCGTCAAG 
GACCCTGGAC GACACCAGCT GGTGGTAAAC AACCCCCCCA GGCCTGTGAG ACATGGAGAC 
ATTGTACAGC TCGTTCACGG CATGACCACC CGCCTGCTTA ACACGCATGA TGTCGCTGCC 
CCGCTGAGCC CCCATTCTCA AGAAGTCTCC TGCTACATTG ACTATAACAT CTCCATGCCT 
GCCCAGAACC TCTGGAAACT GGACATTGTA AACAGAGAGT CCAACCAGGA TACCTGGAAG 
ACTATCTTGT CAGAAGTGCG CTTTGTGCAT GTGAATACAT CTGCCATCTT GAAGCTGAGC 
GGGGCTCACC TCCCTGACTG GGGATTTCGG CAGTTGGAGG TGGTTGGGGA GAAGTTGTCC 
CTCGGCCCCC ACGAGAGCAT GGTATGGAAT GTGGAAGAGC ACCGCTATGG CAGAGGCCAT 
GAGCAGAAGG AGAGGGAGCT GGAGCTCCAC TCACCCACGC AGCATGATAT CAGCAGGAAC 
CTCAGCTTCA TGGCCAGATT CTCGGAGTTA CAGTGGAAGA TGCTGACGCT GAAGAATGAG 
GACTTAGAAC ACCAGTACAG CTCCACCCCG CTGGAGTGGC TCACGCTGGA CACCAACATT 
GCCTATTGGC TGCACCCCAG GACCAGTGCC CAGATCCACT TGCTTGGAAA CATCGTGATC 
TGGACTTCAG CCAGCCTCGC CACAGTGGCA TACACCCTAC TCTTCTTCTG GTACCTGCTC 
CGCCGTCGAA GGAACATCTG TGACCTCCCT GAGGATGCCT GGTCCCACTG GGTGCTGGCT 
GGAGCCCTGT GTATTGGCGG TTGGGCACTC AACTATCTGC CCTTCTTCCT GATGGAAAGG 
ATGCTCTTCC TCTACCACTA CTTGCCGGCC CTCACCTTCC AGATCCTGCT GCTCCCAATC 
GTCATGCAGC ACGCCAGCGA CCATCTGTGC AGGTCCCAGC TGCAGAGGAA TGTCTTCAGT 
GCCCTGGTGG TAGCATGGTA TTCCTCTGCG TGCCATGTGT CCAACATGTT ACGCCCACTG 
ACCTATGGGG ACACGTCACT CTCACCAGGC GAGCTCCGGG CCCTTCGCTG GAAAGACAGC 
TGGGACATTC TCATCCGAAA ATACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pomt1 ( NM_053406.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_445858.1 Rattus norvegicus protein-O-mannosyltransferase 1 (Pomt1), mRNA.

Price & Availability↑

CloneID	ORa09462
Clone ID Related Accession (Same CDS sequence)	NM_053406.2
Accession Version	NM_053406.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2244bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Rattus norvegicus(Norway rat)
Product	protein O-mannosyl-transferase 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DY309994.1, AF192388.1 and BC070912.1. On Oct 12, 2007 this sequence version replaced NM_053406.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF192388.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGGGAACC GCTCAATGGG ACGCGAAGAT ACGCTTGGTG TCCTCCCGAG CTTGCTTTTC 
TGCAAAATGT TGAGATTTTT GAAACGGCCT CTAGTGGTGA CTATTGACAT CAATTTGAAT 
TTGGTGGCTC TGACTGTCCT GGGACTACTT ACCCGGTTAT GGCAACTCTC CTACCCTCGG 
GCTGTGGTTT TTGATGAAGT GTATTACGGG CAGTACATCT CCTTTTACAT GAAGCGTGTC 
TTCTTCCTGG ATGACAGTGG ACCACCGTTC GGCCATATGC TGCTAGCCTT AGGAGGTTGG 
CTAGGAGGAT TCGATGGGAA CTTTCTGTGG AACCGAATTG GAGCAGAATA CAGTAGCAAT 
GTGCCTGTAT GGTCCTTACG CCTGCTGCCG GCGCTTGCTG GGGCCCTGTC AGTACCCATG 
GCCTACCAGA TAGTGTTGGA GCTCCACTTT TCCCACTGTA CTGCCATGGG AGCCGCCCTG 
CTGATGCTCA TTGAGAACGC CCTAATCACT CAGTCCAGGC TCATGCTGTT GGAATCCATA 
CTGATATTTT TTAACCTCTT GGCCGTGTTG TCCTATCTGA AGTTCTTCAA CTCCCAAACA 
CACAGCCCTT TCTCAGTGCA CTGGTGGCTG TGGCTAATGC TGACCGGAGT CTCTTGTTCC 
TGTGCGGTTG GGATCAAGTA CATGGGCATT TTCACCTACT TGCTCGTGCT CAGCATTGCA 
GCTGTGCATG CCTGGCACCT GATCGGAGAC CAGACCTTGT CAAATATCTG CGTGCTCAGT 
CACTTGCTCG CCAGAGCCGT AGCTCTGCTG GTCGTCCCGG TCTTCCTGTA CTTACTGTTC 
TTCTATGTCC ACCTGATGTT GCTCTACCGC TCTGGGCCCC ATGACCAAAT CATGTCCAGT 
GCCTTCCAAG CCAGCTTGGA GGGAGGGCTA GCCCGCATCA CCCAAGGCCA GCCCCTAGAG 
GTGGCCTTTG GTTCGCAGGT CACTCTGAAG AGCGTCTCCG GCAAACCCTT GCCCTGCTGG 
CTTCATTCGC ACAAGAACAC CTATCCCATG ATATATGAGA ATGGCCGTGG CAGCTCCCAC 
CAGCAACAGG TGACCTGTTA TCCCTTCAAA GACATCAATA ACTGGTGGAT CGTCAAGGAC 
CCTGGACGAC ACCAGCTGGT GGTAAACAAC CCCCCCAGGC CTGTGAGACA TGGAGACATT 
GTACAGCTCG TTCACGGCAT GACCACCCGC CTGCTTAACA CGCATGATGT CGCTGCCCCG 
CTGAGCCCCC ATTCTCAAGA AGTCTCCTGC TACATTGACT ATAACATCTC CATGCCTGCC 
CAGAACCTCT GGAAACTGGA CATTGTAAAC AGAGAGTCCA ACCAGGATAC CTGGAAGACT 
ATCTTGTCAG AAGTGCGCTT TGTGCATGTG AATACATCTG CCATCTTGAA GCTGAGCGGG 
GCTCACCTCC CTGACTGGGG ATTTCGGCAG TTGGAGGTGG TTGGGGAGAA GTTGTCCCTC 
GGCCCCCACG AGAGCATGGT ATGGAATGTG GAAGAGCACC GCTATGGCAG AGGCCATGAG 
CAGAAGGAGA GGGAGCTGGA GCTCCACTCA CCCACGCAGC ATGATATCAG CAGGAACCTC 
AGCTTCATGG CCAGATTCTC GGAGTTACAG TGGAAGATGC TGACGCTGAA GAATGAGGAC 
TTAGAACACC AGTACAGCTC CACCCCGCTG GAGTGGCTCA CGCTGGACAC CAACATTGCC 
TATTGGCTGC ACCCCAGGAC CAGTGCCCAG ATCCACTTGC TTGGAAACAT CGTGATCTGG 
ACTTCAGCCA GCCTCGCCAC AGTGGCATAC ACCCTACTCT TCTTCTGGTA CCTGCTCCGC 
CGTCGAAGGA ACATCTGTGA CCTCCCTGAG GATGCCTGGT CCCACTGGGT GCTGGCTGGA 
GCCCTGTGTA TTGGCGGTTG GGCACTCAAC TATCTGCCCT TCTTCCTGAT GGAAAGGATG 
CTCTTCCTCT ACCACTACTT GCCGGCCCTC ACCTTCCAGA TCCTGCTGCT CCCAATCGTC 
ATGCAGCACG CCAGCGACCA TCTGTGCAGG TCCCAGCTGC AGAGGAATGT CTTCAGTGCC 
CTGGTGGTAG CATGGTATTC CTCTGCGTGC CATGTGTCCA ACATGTTACG CCCACTGACC 
TATGGGGACA CGTCACTCTC ACCAGGCGAG CTCCGGGCCC TTCGCTGGAA AGACAGCTGG 
GACATTCTCA TCCGAAAATA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_445858.1
CDS	89..2332
Translation	MGNRSMGREDTLGVLPSLLFCKMLRFLKRPLVVTIDINLNLVALTVLGLLTRLWQLSYPRAVVFDEVYYGQYISFYMKRVFFLDDSGPPFGHMLLALGGWLGGFDGNFLWNRIGAEYSSNVPVWSLRLLPALAGALSVPMAYQIVLELHFSHCTAMGAALLMLIENALITQSRLMLLESILIFFNLLAVLSYLKFFNSQTHSPFSVHWWLWLMLTGVSCSCAVGIKYMGIFTYLLVLSIAAVHAWHLIGDQTLSNICVLSHLLARAVALLVVPVFLYLLFFYVHLMLLYRSGPHDQIMSSAFQASLEGGLARITQGQPLEVAFGSQVTLKSVSGKPLPCWLHSHKNTYPMIYENGRGSSHQQQVTCYPFKDINNWWIVKDPGRHQLVVNNPPRPVRHGDIVQLVHGMTTRLLNTHDVAAPLSPHSQEVSCYIDYNISMPAQNLWKLDIVNRESNQDTWKTILSEVRFVHVNTSAILKLSGAHLPDWGFRQLEVVGEKLSLGPHESMVWNVEEHRYGRGHEQKERELELHSPTQHDISRNLSFMARFSELQWKMLTLKNEDLEHQYSSTPLEWLTLDTNIAYWLHPRTSAQIHLLGNIVIWTSASLATVAYTLLFFWYLLRRRRNICDLPEDAWSHWVLAGALCIGGWALNYLPFFLMERMLFLYHYLPALTFQILLLPIVMQHASDHLCRSQLQRNVFSALVVAWYSSACHVSNMLRPLTYGDTSLSPGELRALRWKDSWDILIRKY

Target ORF information:

RefSeq Version	NM_053406.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus protein-O-mannosyltransferase 1 (Pomt1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_053406.2

ORF Insert Sequence:

ATGGGGAACC GCTCAATGGG ACGCGAAGAT ACGCTTGGTG TCCTCCCGAG CTTGCTTTTC 
TGCAAAATGT TGAGATTTTT GAAACGGCCT CTAGTGGTGA CTATTGACAT CAATTTGAAT 
TTGGTGGCTC TGACTGTCCT GGGACTACTT ACCCGGTTAT GGCAACTCTC CTACCCTCGG 
GCTGTGGTTT TTGATGAAGT GTATTACGGG CAGTACATCT CCTTTTACAT GAAGCGTGTC 
TTCTTCCTGG ATGACAGTGG ACCACCGTTC GGCCATATGC TGCTAGCCTT AGGAGGTTGG 
CTAGGAGGAT TCGATGGGAA CTTTCTGTGG AACCGAATTG GAGCAGAATA CAGTAGCAAT 
GTGCCTGTAT GGTCCTTACG CCTGCTGCCG GCGCTTGCTG GGGCCCTGTC AGTACCCATG 
GCCTACCAGA TAGTGTTGGA GCTCCACTTT TCCCACTGTA CTGCCATGGG AGCCGCCCTG 
CTGATGCTCA TTGAGAACGC CCTAATCACT CAGTCCAGGC TCATGCTGTT GGAATCCATA 
CTGATATTTT TTAACCTCTT GGCCGTGTTG TCCTATCTGA AGTTCTTCAA CTCCCAAACA 
CACAGCCCTT TCTCAGTGCA CTGGTGGCTG TGGCTAATGC TGACCGGAGT CTCTTGTTCC 
TGTGCGGTTG GGATCAAGTA CATGGGCATT TTCACCTACT TGCTCGTGCT CAGCATTGCA 
GCTGTGCATG CCTGGCACCT GATCGGAGAC CAGACCTTGT CAAATATCTG CGTGCTCAGT 
CACTTGCTCG CCAGAGCCGT AGCTCTGCTG GTCGTCCCGG TCTTCCTGTA CTTACTGTTC 
TTCTATGTCC ACCTGATGTT GCTCTACCGC TCTGGGCCCC ATGACCAAAT CATGTCCAGT 
GCCTTCCAAG CCAGCTTGGA GGGAGGGCTA GCCCGCATCA CCCAAGGCCA GCCCCTAGAG 
GTGGCCTTTG GTTCGCAGGT CACTCTGAAG AGCGTCTCCG GCAAACCCTT GCCCTGCTGG 
CTTCATTCGC ACAAGAACAC CTATCCCATG ATATATGAGA ATGGCCGTGG CAGCTCCCAC 
CAGCAACAGG TGACCTGTTA TCCCTTCAAA GACATCAATA ACTGGTGGAT CGTCAAGGAC 
CCTGGACGAC ACCAGCTGGT GGTAAACAAC CCCCCCAGGC CTGTGAGACA TGGAGACATT 
GTACAGCTCG TTCACGGCAT GACCACCCGC CTGCTTAACA CGCATGATGT CGCTGCCCCG 
CTGAGCCCCC ATTCTCAAGA AGTCTCCTGC TACATTGACT ATAACATCTC CATGCCTGCC 
CAGAACCTCT GGAAACTGGA CATTGTAAAC AGAGAGTCCA ACCAGGATAC CTGGAAGACT 
ATCTTGTCAG AAGTGCGCTT TGTGCATGTG AATACATCTG CCATCTTGAA GCTGAGCGGG 
GCTCACCTCC CTGACTGGGG ATTTCGGCAG TTGGAGGTGG TTGGGGAGAA GTTGTCCCTC 
GGCCCCCACG AGAGCATGGT ATGGAATGTG GAAGAGCACC GCTATGGCAG AGGCCATGAG 
CAGAAGGAGA GGGAGCTGGA GCTCCACTCA CCCACGCAGC ATGATATCAG CAGGAACCTC 
AGCTTCATGG CCAGATTCTC GGAGTTACAG TGGAAGATGC TGACGCTGAA GAATGAGGAC 
TTAGAACACC AGTACAGCTC CACCCCGCTG GAGTGGCTCA CGCTGGACAC CAACATTGCC 
TATTGGCTGC ACCCCAGGAC CAGTGCCCAG ATCCACTTGC TTGGAAACAT CGTGATCTGG 
ACTTCAGCCA GCCTCGCCAC AGTGGCATAC ACCCTACTCT TCTTCTGGTA CCTGCTCCGC 
CGTCGAAGGA ACATCTGTGA CCTCCCTGAG GATGCCTGGT CCCACTGGGT GCTGGCTGGA 
GCCCTGTGTA TTGGCGGTTG GGCACTCAAC TATCTGCCCT TCTTCCTGAT GGAAAGGATG 
CTCTTCCTCT ACCACTACTT GCCGGCCCTC ACCTTCCAGA TCCTGCTGCT CCCAATCGTC 
ATGCAGCACG CCAGCGACCA TCTGTGCAGG TCCCAGCTGC AGAGGAATGT CTTCAGTGCC 
CTGGTGGTAG CATGGTATTC CTCTGCGTGC CATGTGTCCA ACATGTTACG CCCACTGACC 
TATGGGGACA CGTCACTCTC ACCAGGCGAG CTCCGGGCCC TTCGCTGGAA AGACAGCTGG 
GACATTCTCA TCCGAAAATA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mammalian
The Journal of biological chemistry292(27)11586-11598(2017 07)
Larsen ISB,Narimatsu Y,Joshi HJ,Yang Z,Harrison OJ,Brasch J,Shapiro L,Honig B,Vakhrushev SY,Clausen H,Halim A

Cardiomyopathy in patients with POMT1-related congenital and limb-girdle muscular dystrophy.
European journal of human genetics : EJHG20(12)1234-9(2012 Dec)
Bello L,Melacini P,Pezzani R,D'Amico A,Piva L,Leonardi E,Torella A,Soraru G,Palmieri A,Smaniotto G,Gavassini BF,Vianello A,Nigro V,Bertini E,Angelini C,Tosatto SC,Pegoraro E

Walker-Warburg Syndrome with POMT1 mutations can be associated with cleft lip and cleft palate.
Neuromuscular disorders : NMD18(8)675-7(2008 Aug)
Vajsar J,Baskin B,Swoboda K,Biggar DW,Schachter H,Ray PN

Molecular heterogeneity in fetal forms of type II lissencephaly.
Human mutation28(10)1020-7(2007 Oct)
Bouchet C,Gonzales M,Vuillaumier-Barrot S,Devisme L,Lebizec C,Alanio E,Bazin A,Bessi?res-Grattagliano B,Bigi N,Blanchet P,Bonneau D,Bonni?res M,Carles D,Delahaye S,Fallet-Bianco C,Figarella-Branger D,Gaillard D,Gasser B,Guimiot F,Joubert M,Laurent N,Liprandi A,Loget P,Marcorelles P,Martinovic J,Menez F,Patrier S,Pelluard-Nehm? F,Perez MJ,Rouleau-Dubois C,Triau S,Laquerri?re A,Encha-Razavi F,Seta N

Expression of the murine Pomt1 gene in both the developing brain and adult muscle tissues and its relationship with clinical aspects of Walker-Warburg syndrome.
The American journal of pathology170(5)1659-68(2007 May)
Prados B,Pe?a A,Cotarelo RP,Valero MC,Cruces J

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Pomt1 cDNA ORF clone, Rattus norvegicus(Norway rat)

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