Dcx cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Dcx
Entrez Gene ID	84394
Full Name	doublecortin
General protein information	Preferred Names doublecortin Names neuronal migration protein doublecortin
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: X Map Location: Xq33-q34
Summary	This gene encodes a member of the doublecortin family. The protein encoded by this gene contains two doublecortin domains, which bind microtubules and regulate microtubule polymerization. In the developing cortex, cortical neurons must migrate over long distances to reach the site of their final differentiation. The encoded protein appears to direct neuronal migration by regulating the organization and stability of microtubules. Studies in knockout mice lacking this gene suggest that this gene has a cortical role in nuclear translocation and positioning of the mitotic spindle in radial glial mitotic division. This gene is essential for neuronal migration, differentiation, and plasticity, is required for hippocampal development and also plays a role in dendrites development.[provided by RefSeq, Sep 2010].

mRNA and Protein(s)

mRNA	Protein	Name
XM_006257385.3	XP_006257447.1	neuronal migration protein doublecortin isoform X1
XM_017602167.1	XP_017457656.1	neuronal migration protein doublecortin isoform X1
XM_006257383.3	XP_006257445.1	neuronal migration protein doublecortin isoform X3
NM_053379.3	NP_445831.3	neuronal migration protein doublecortin
XM_006257384.3	XP_006257446.1	neuronal migration protein doublecortin isoform X4
XM_006257386.3	XP_006257448.1	neuronal migration protein doublecortin isoform X2
XM_006257382.2	XP_006257444.1	neuronal migration protein doublecortin isoform X1

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Dcx	NP_445831.3
Homo sapiens (human)	DCX	NP_000546.2
Pan troglodytes (chimpanzee)	DCX	XP_003317682.1
	DCX	XP_001100519.2
Bos taurus (cattle)	DCX	NP_001193894.1
Xenopus tropicalis (tropical clawed frog)	dcx	XP_004916904.1
Canis lupus familiaris (dog)	DCX	XP_005641756.1
Gallus gallus (chicken)	DCX	NP_989666.1
Mus musculus (house mouse)	Dcx	NP_001103693.1

Gene Ontology
Bibliography

Related articles in PubMed

A novel DCX missense mutation in a family with X-linked lissencephaly and subcortical band heterotopia syndrome inherited from a low-level somatic mosaic mother: Genetic and functional studies.
Tsai MH, Kuo PW, Myers CT, Li SW, Lin WC, Fu TY, Chang HY, Mefford HC, Chang YC, Tsai JW
European journal of paediatric neurology : EJPN : official journal of the European Paediatric Neurology Society20(5)788-94(2016 Sep)

[EXPRESSION OF DOUBLECORTIN AND NeuN IN THE DEVELOPING CEREBELLAR NEURONS IN RAT].
Zimatkin SM, Karniushko OA
Morfologiia (Saint Petersburg, Russia)149(1)38-42(2016)

Disruption of Visc-2, a Brain-Expressed Conserved Long Noncoding RNA, Does Not Elicit an Overt Anatomical or Behavioral Phenotype.
Oliver PL, Chodroff RA, Gosal A, Edwards B, Cheung AF, Gomez-Rodriguez J, Elliot G, Garrett LJ, Lickiss T, Szele F, Green ED, Moln?r Z, Ponting CP
Cerebral cortex (New York, N.Y. : 1991)25(10)3572-85(2015 Oct)

Time course of spinal doublecortin expression in developing rat and porcine spinal cord: implication in in vivo neural precursor grafting studies.
Juhasova J, Juhas S, Hruska-Plochan M, Dolezalova D, Holubova M, Strnadel J, Marsala S, Motlik J, Marsala M
Cellular and molecular neurobiology35(1)57-70(2015 Jan)

Protein kinase LKB1 regulates polarized dendrite formation of adult hippocampal newborn neurons.
Huang W, She L, Chang XY, Yang RR, Wang L, Ji HB, Jiao JW, Poo MM
Proceedings of the National Academy of Sciences of the United States of America111(1)469-74(2014 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

EXPRESSION OF DOUBLECORTIN AND NeuN IN THE DEVELOPING CEREBELLAR NEURONS IN RAT
Title: [EXPRESSION OF DOUBLECORTIN AND NeuN IN THE DEVELOPING CEREBELLAR NEURONS IN RAT].

The expression of Dcx was found to be a reliable marker of grafted neurons in spinal cord.
Title: Time course of spinal doublecortin expression in developing rat and porcine spinal cord: implication in in vivo neural precursor grafting studies.

The total number of DCX-expressing cells was 1,032, 1,219, and 1,904 in Group P, Group G, and Group C, respectively.
Title: Acute stress exposure preceding global brain ischemia accelerates decreased doublecortin expression in the rat retrosplenial cortex.

Dcx protein- and mRNA-levels reflected changes in neurogenesis
Title: Analysis of adult neurogenesis: evidence for a prominent "non-neurogenic" DCX-protein pool in rodent brain.

DCX+DCLK depletion alters the behavior of growth cones at substrate borders.
Title: A novel role for doublecortin and doublecortin-like kinase in regulating growth cone microtubules.

The following Dcx gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dcx cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa13364	NM_053379.3 Latest version!	Rattus norvegicus doublecortin (Dcx), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
ORa40151	XM_006257385.3 Latest version!	Rattus norvegicus doublecortin (Dcx), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
ORa40151	XM_006257382.2 Latest version!	Rattus norvegicus doublecortin (Dcx), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
ORa40151	XM_017602167.1 Latest version!	Rattus norvegicus doublecortin (Dcx), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
ORa40152	XM_006257383.3 Latest version!	Rattus norvegicus doublecortin (Dcx), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
ORa40153	XM_006257384.3 Latest version!	Rattus norvegicus doublecortin (Dcx), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
ORa40154	XM_006257386.3 Latest version!	Rattus norvegicus doublecortin (Dcx), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dcx ( NM_053379.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_445831.3 Rattus norvegicus doublecortin (Dcx), mRNA.

Price & Availability↑

CloneID	ORa13364
Clone ID Related Accession (Same CDS sequence)	NM_053379.3
Accession Version	NM_053379.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1098bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Rattus norvegicus(Norway rat)
Product	neuronal migration protein doublecortin
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AABR07040934.1 and AABR07073541.1. On Sep 30, 2010 this sequence version replaced NM_053379.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: AF155959.2 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGAACTTG ATTTTGGACA TTTTGACGAA CGAGACAAAG CATCTAGAAA TATGAGAGGG 
TCACGGATGA ATGGACTTCC AAGTCCCACT CATAGTGCTC ACTGTAGCTT CTACAGAACC 
AGAACCTTGC AGGCATTAAG TAATGAGAAG AAGGCCAAGA AGGTACGTTT CTACCGCAAT 
GGGGACCGTT ACTTCAAGGG GATTGTGTAC GCTGTTTCTT CTGACCGTTT TCGTAGTTTT 
GATGCATTGC TGGCTGACCT GACTCGATCC TTGTCCGACA ACATCAACCT GCCTCAGGGA 
GTGCGCTACA TTTACACTAT TGATGGATCC AGAAAGATTG GAAGCATGGA TGAACTGGAA 
GAAGGGGAAA GCTATGTCTG TTCCTCAGAC AACTTCTTTA AAAAGGTTGA GTATACCAAG 
AATGTCAATC CCAACTGGTC GGTCAATGTA AAGACATCAG CCAACATGAA AGCGCCCCAG 
TCCTTGGCTA GCAGCAACAG TGCTCAAGCC AGAGAGAACA AGGACTTCGT GCGCCCCAAA 
CTTGTGACCA TCATTCGCAG TGGGGTGAAG CCTCGGAAGG CGGTGCGTGT GCTTCTCAAC 
AAGAAAACAG CCCACTCTTT CGAGCAGGTC CTCACTGACA TCACAGAAGC GATCAAACTG 
GAAACCGGAG TTGTCAAAAA ACTCTATACC CTTGATGGAA AGCAGGTCAC CTGTCTCCAT 
GATTTCTTTG GTGATGATGA TGTGTTCATT GCTTGTGGCC CTGAAAAGTT CCGCTATGCT 
CAAGATGACT TCTCCTTGGA TGAGAATGAA TGCAGAGTCA TGAAAGGGAA TCCATCTGCC 
ACAGCTGGCC CAAAAGCTTC CCCAACACCT CAAAAGACAT CTGCTAAAAG CCCAGGCCCA 
ATGCGCCGCA GCAAGTCTCC AGCTGACTCA GGTAACGACC AAGACGCAAA TGGAACTTCT 
AGCAGTCAGC TCTCAACACC TAAGTCGAAG CAGTCTCCTA TCTCTACACC CACAAGCCCT 
GGAAGTCTGC GGAAGCACAA GGACTTGTAC CTGCCACTGT CATTGGATGA CTCTGATTCA 
CTTGGCGATT CCATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_445831.3
CDS	67..1164
Translation	MELDFGHFDERDKASRNMRGSRMNGLPSPTHSAHCSFYRTRTLQALSNEKKAKKVRFYRNGDRYFKGIVYAVSSDRFRSFDALLADLTRSLSDNINLPQGVRYIYTIDGSRKIGSMDELEEGESYVCSSDNFFKKVEYTKNVNPNWSVNVKTSANMKAPQSLASSNSAQARENKDFVRPKLVTIIRSGVKPRKAVRVLLNKKTAHSFEQVLTDITEAIKLETGVVKKLYTLDGKQVTCLHDFFGDDDVFIACGPEKFRYAQDDFSLDENECRVMKGNPSATAGPKASPTPQKTSAKSPGPMRRSKSPADSGNDQDANGTSSSQLSTPKSKQSPISTPTSPGSLRKHKDLYLPLSLDDSDSLGDSM

Target ORF information:

RefSeq Version	NM_053379.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus doublecortin (Dcx), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_053379.3

ORF Insert Sequence:

ATGGAACTTG ATTTTGGACA TTTTGACGAA CGAGACAAAG CATCTAGAAA TATGAGAGGG 
TCACGGATGA ATGGACTTCC AAGTCCCACT CATAGTGCTC ACTGTAGCTT CTACAGAACC 
AGAACCTTGC AGGCATTAAG TAATGAGAAG AAGGCCAAGA AGGTACGTTT CTACCGCAAT 
GGGGACCGTT ACTTCAAGGG GATTGTGTAC GCTGTTTCTT CTGACCGTTT TCGTAGTTTT 
GATGCATTGC TGGCTGACCT GACTCGATCC TTGTCCGACA ACATCAACCT GCCTCAGGGA 
GTGCGCTACA TTTACACTAT TGATGGATCC AGAAAGATTG GAAGCATGGA TGAACTGGAA 
GAAGGGGAAA GCTATGTCTG TTCCTCAGAC AACTTCTTTA AAAAGGTTGA GTATACCAAG 
AATGTCAATC CCAACTGGTC GGTCAATGTA AAGACATCAG CCAACATGAA AGCGCCCCAG 
TCCTTGGCTA GCAGCAACAG TGCTCAAGCC AGAGAGAACA AGGACTTCGT GCGCCCCAAA 
CTTGTGACCA TCATTCGCAG TGGGGTGAAG CCTCGGAAGG CGGTGCGTGT GCTTCTCAAC 
AAGAAAACAG CCCACTCTTT CGAGCAGGTC CTCACTGACA TCACAGAAGC GATCAAACTG 
GAAACCGGAG TTGTCAAAAA ACTCTATACC CTTGATGGAA AGCAGGTCAC CTGTCTCCAT 
GATTTCTTTG GTGATGATGA TGTGTTCATT GCTTGTGGCC CTGAAAAGTT CCGCTATGCT 
CAAGATGACT TCTCCTTGGA TGAGAATGAA TGCAGAGTCA TGAAAGGGAA TCCATCTGCC 
ACAGCTGGCC CAAAAGCTTC CCCAACACCT CAAAAGACAT CTGCTAAAAG CCCAGGCCCA 
ATGCGCCGCA GCAAGTCTCC AGCTGACTCA GGTAACGACC AAGACGCAAA TGGAACTTCT 
AGCAGTCAGC TCTCAACACC TAAGTCGAAG CAGTCTCCTA TCTCTACACC CACAAGCCCT 
GGAAGTCTGC GGAAGCACAA GGACTTGTAC CTGCCACTGT CATTGGATGA CTCTGATTCA 
CTTGGCGATT CCATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dcx ( XM_006257385.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006257447.1 Rattus norvegicus doublecortin (Dcx), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa40151
Clone ID Related Accession (Same CDS sequence)	XM_006257385.3 , XM_017602167.1 , XM_006257382.2
Accession Version	XM_006257385.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1101bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	neuronal migration protein doublecortin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005120.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006257385.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAACTTG ATTTTGGACA TTTTGACGAA CGAGACAAAG CATCTAGAAA TATGAGAGGG 
TCACGGATGA ATGGACTTCC AAGTCCCACT CATAGTGCTC ACTGTAGCTT CTACAGAACC 
AGAACCTTGC AGGCATTAAG TAATGAGAAG AAGGCCAAGA AGGTACGTTT CTACCGCAAT 
GGGGACCGTT ACTTCAAGGG GATTGTGTAC GCTGTTTCTT CTGACCGTTT TCGTAGTTTT 
GATGCATTGC TGGCTGACCT GACTCGATCC TTGTCCGACA ACATCAACCT GCCTCAGGGA 
GTGCGCTACA TTTACACTAT TGATGGATCC AGAAAGATTG GAAGCATGGA TGAACTGGAA 
GAAGGGGAAA GCTATGTCTG TTCCTCAGAC AACTTCTTTA AAAAGGTTGA GTATACCAAG 
AATGTCAATC CCAACTGGTC GGTCAATGTA AAGACATCAG CCAACATGAA AGCGCCCCAG 
TCCTTGGCTA GCAGCAACAG TGCTCAAGCC AGAGAGAACA AGGACTTCGT GCGCCCCAAA 
CTTGTGACCA TCATTCGCAG TGGGGTGAAG CCTCGGAAGG CGGTGCGTGT GCTTCTCAAC 
AAGAAAACAG CCCACTCTTT CGAGCAGGTC CTCACTGACA TCACAGAAGC GATCAAACTG 
GAAACCGGAG TTGTCAAAAA ACTCTATACC CTTGATGGAA AGCAGGTCAC CTGTCTCCAT 
GATTTCTTTG GTGATGATGA TGTGTTCATT GCTTGTGGCC CTGAAAAGTT CCGCTATGCT 
CAAGATGACT TCTCCTTGGA TGAGAATGAA TGCAGAGTCA TGAAAGGGAA TCCATCTGCC 
ACAGCTGGCC CAAAAGCTTC CCCAACACCT CAAAAGACAT CTGCTAAAAG CCCAGGCCCA 
ATGCGCCGCA GCAAGTCTCC AGCTGACTCA GGTAACGACC AAGACGCAAA TGGAACTTCT 
AGCAGTCAGC TCTCAACACC TAAGTCGAAG CAGTCTCCTA TCTCTACACC CACAAGCCCT 
GGAAGTCTGC GGAAGCACAA GGTAGACTTG TACCTGCCAC TGTCATTGGA TGACTCTGAT 
TCACTTGGCG ATTCCATGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006257447.1
CDS	282..1382
Translation	MELDFGHFDERDKASRNMRGSRMNGLPSPTHSAHCSFYRTRTLQALSNEKKAKKVRFYRNGDRYFKGIVYAVSSDRFRSFDALLADLTRSLSDNINLPQGVRYIYTIDGSRKIGSMDELEEGESYVCSSDNFFKKVEYTKNVNPNWSVNVKTSANMKAPQSLASSNSAQARENKDFVRPKLVTIIRSGVKPRKAVRVLLNKKTAHSFEQVLTDITEAIKLETGVVKKLYTLDGKQVTCLHDFFGDDDVFIACGPEKFRYAQDDFSLDENECRVMKGNPSATAGPKASPTPQKTSAKSPGPMRRSKSPADSGNDQDANGTSSSQLSTPKSKQSPISTPTSPGSLRKHKVDLYLPLSLDDSDSLGDSM

Target ORF information:

RefSeq Version	XM_006257385.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus doublecortin (Dcx), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006257385.3

ORF Insert Sequence:

ATGGAACTTG ATTTTGGACA TTTTGACGAA CGAGACAAAG CATCTAGAAA TATGAGAGGG 
TCACGGATGA ATGGACTTCC AAGTCCCACT CATAGTGCTC ACTGTAGCTT CTACAGAACC 
AGAACCTTGC AGGCATTAAG TAATGAGAAG AAGGCCAAGA AGGTACGTTT CTACCGCAAT 
GGGGACCGTT ACTTCAAGGG GATTGTGTAC GCTGTTTCTT CTGACCGTTT TCGTAGTTTT 
GATGCATTGC TGGCTGACCT GACTCGATCC TTGTCCGACA ACATCAACCT GCCTCAGGGA 
GTGCGCTACA TTTACACTAT TGATGGATCC AGAAAGATTG GAAGCATGGA TGAACTGGAA 
GAAGGGGAAA GCTATGTCTG TTCCTCAGAC AACTTCTTTA AAAAGGTTGA GTATACCAAG 
AATGTCAATC CCAACTGGTC GGTCAATGTA AAGACATCAG CCAACATGAA AGCGCCCCAG 
TCCTTGGCTA GCAGCAACAG TGCTCAAGCC AGAGAGAACA AGGACTTCGT GCGCCCCAAA 
CTTGTGACCA TCATTCGCAG TGGGGTGAAG CCTCGGAAGG CGGTGCGTGT GCTTCTCAAC 
AAGAAAACAG CCCACTCTTT CGAGCAGGTC CTCACTGACA TCACAGAAGC GATCAAACTG 
GAAACCGGAG TTGTCAAAAA ACTCTATACC CTTGATGGAA AGCAGGTCAC CTGTCTCCAT 
GATTTCTTTG GTGATGATGA TGTGTTCATT GCTTGTGGCC CTGAAAAGTT CCGCTATGCT 
CAAGATGACT TCTCCTTGGA TGAGAATGAA TGCAGAGTCA TGAAAGGGAA TCCATCTGCC 
ACAGCTGGCC CAAAAGCTTC CCCAACACCT CAAAAGACAT CTGCTAAAAG CCCAGGCCCA 
ATGCGCCGCA GCAAGTCTCC AGCTGACTCA GGTAACGACC AAGACGCAAA TGGAACTTCT 
AGCAGTCAGC TCTCAACACC TAAGTCGAAG CAGTCTCCTA TCTCTACACC CACAAGCCCT 
GGAAGTCTGC GGAAGCACAA GGTAGACTTG TACCTGCCAC TGTCATTGGA TGACTCTGAT 
TCACTTGGCG ATTCCATGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dcx ( XM_017602167.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017457656.1 Rattus norvegicus doublecortin (Dcx), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa40151
Clone ID Related Accession (Same CDS sequence)	XM_006257385.3 , XM_017602167.1 , XM_006257382.2
Accession Version	XM_017602167.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1101bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	neuronal migration protein doublecortin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005120.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAACTTG ATTTTGGACA TTTTGACGAA CGAGACAAAG CATCTAGAAA TATGAGAGGG 
TCACGGATGA ATGGACTTCC AAGTCCCACT CATAGTGCTC ACTGTAGCTT CTACAGAACC 
AGAACCTTGC AGGCATTAAG TAATGAGAAG AAGGCCAAGA AGGTACGTTT CTACCGCAAT 
GGGGACCGTT ACTTCAAGGG GATTGTGTAC GCTGTTTCTT CTGACCGTTT TCGTAGTTTT 
GATGCATTGC TGGCTGACCT GACTCGATCC TTGTCCGACA ACATCAACCT GCCTCAGGGA 
GTGCGCTACA TTTACACTAT TGATGGATCC AGAAAGATTG GAAGCATGGA TGAACTGGAA 
GAAGGGGAAA GCTATGTCTG TTCCTCAGAC AACTTCTTTA AAAAGGTTGA GTATACCAAG 
AATGTCAATC CCAACTGGTC GGTCAATGTA AAGACATCAG CCAACATGAA AGCGCCCCAG 
TCCTTGGCTA GCAGCAACAG TGCTCAAGCC AGAGAGAACA AGGACTTCGT GCGCCCCAAA 
CTTGTGACCA TCATTCGCAG TGGGGTGAAG CCTCGGAAGG CGGTGCGTGT GCTTCTCAAC 
AAGAAAACAG CCCACTCTTT CGAGCAGGTC CTCACTGACA TCACAGAAGC GATCAAACTG 
GAAACCGGAG TTGTCAAAAA ACTCTATACC CTTGATGGAA AGCAGGTCAC CTGTCTCCAT 
GATTTCTTTG GTGATGATGA TGTGTTCATT GCTTGTGGCC CTGAAAAGTT CCGCTATGCT 
CAAGATGACT TCTCCTTGGA TGAGAATGAA TGCAGAGTCA TGAAAGGGAA TCCATCTGCC 
ACAGCTGGCC CAAAAGCTTC CCCAACACCT CAAAAGACAT CTGCTAAAAG CCCAGGCCCA 
ATGCGCCGCA GCAAGTCTCC AGCTGACTCA GGTAACGACC AAGACGCAAA TGGAACTTCT 
AGCAGTCAGC TCTCAACACC TAAGTCGAAG CAGTCTCCTA TCTCTACACC CACAAGCCCT 
GGAAGTCTGC GGAAGCACAA GGTAGACTTG TACCTGCCAC TGTCATTGGA TGACTCTGAT 
TCACTTGGCG ATTCCATGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017457656.1
CDS	331..1431
Translation	MELDFGHFDERDKASRNMRGSRMNGLPSPTHSAHCSFYRTRTLQALSNEKKAKKVRFYRNGDRYFKGIVYAVSSDRFRSFDALLADLTRSLSDNINLPQGVRYIYTIDGSRKIGSMDELEEGESYVCSSDNFFKKVEYTKNVNPNWSVNVKTSANMKAPQSLASSNSAQARENKDFVRPKLVTIIRSGVKPRKAVRVLLNKKTAHSFEQVLTDITEAIKLETGVVKKLYTLDGKQVTCLHDFFGDDDVFIACGPEKFRYAQDDFSLDENECRVMKGNPSATAGPKASPTPQKTSAKSPGPMRRSKSPADSGNDQDANGTSSSQLSTPKSKQSPISTPTSPGSLRKHKVDLYLPLSLDDSDSLGDSM

Target ORF information:

RefSeq Version	XM_017602167.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus doublecortin (Dcx), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017602167.1

ORF Insert Sequence:

ATGGAACTTG ATTTTGGACA TTTTGACGAA CGAGACAAAG CATCTAGAAA TATGAGAGGG 
TCACGGATGA ATGGACTTCC AAGTCCCACT CATAGTGCTC ACTGTAGCTT CTACAGAACC 
AGAACCTTGC AGGCATTAAG TAATGAGAAG AAGGCCAAGA AGGTACGTTT CTACCGCAAT 
GGGGACCGTT ACTTCAAGGG GATTGTGTAC GCTGTTTCTT CTGACCGTTT TCGTAGTTTT 
GATGCATTGC TGGCTGACCT GACTCGATCC TTGTCCGACA ACATCAACCT GCCTCAGGGA 
GTGCGCTACA TTTACACTAT TGATGGATCC AGAAAGATTG GAAGCATGGA TGAACTGGAA 
GAAGGGGAAA GCTATGTCTG TTCCTCAGAC AACTTCTTTA AAAAGGTTGA GTATACCAAG 
AATGTCAATC CCAACTGGTC GGTCAATGTA AAGACATCAG CCAACATGAA AGCGCCCCAG 
TCCTTGGCTA GCAGCAACAG TGCTCAAGCC AGAGAGAACA AGGACTTCGT GCGCCCCAAA 
CTTGTGACCA TCATTCGCAG TGGGGTGAAG CCTCGGAAGG CGGTGCGTGT GCTTCTCAAC 
AAGAAAACAG CCCACTCTTT CGAGCAGGTC CTCACTGACA TCACAGAAGC GATCAAACTG 
GAAACCGGAG TTGTCAAAAA ACTCTATACC CTTGATGGAA AGCAGGTCAC CTGTCTCCAT 
GATTTCTTTG GTGATGATGA TGTGTTCATT GCTTGTGGCC CTGAAAAGTT CCGCTATGCT 
CAAGATGACT TCTCCTTGGA TGAGAATGAA TGCAGAGTCA TGAAAGGGAA TCCATCTGCC 
ACAGCTGGCC CAAAAGCTTC CCCAACACCT CAAAAGACAT CTGCTAAAAG CCCAGGCCCA 
ATGCGCCGCA GCAAGTCTCC AGCTGACTCA GGTAACGACC AAGACGCAAA TGGAACTTCT 
AGCAGTCAGC TCTCAACACC TAAGTCGAAG CAGTCTCCTA TCTCTACACC CACAAGCCCT 
GGAAGTCTGC GGAAGCACAA GGTAGACTTG TACCTGCCAC TGTCATTGGA TGACTCTGAT 
TCACTTGGCG ATTCCATGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dcx ( XM_006257382.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006257444.1 Rattus norvegicus doublecortin (Dcx), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa40151
Clone ID Related Accession (Same CDS sequence)	XM_006257385.3 , XM_017602167.1 , XM_006257382.2
Accession Version	XM_006257382.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1101bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	neuronal migration protein doublecortin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005120.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 7, 2014 this sequence version replaced XM_006257382.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAACTTG ATTTTGGACA TTTTGACGAA CGAGACAAAG CATCTAGAAA TATGAGAGGG 
TCACGGATGA ATGGACTTCC AAGTCCCACT CATAGTGCTC ACTGTAGCTT CTACAGAACC 
AGAACCTTGC AGGCATTAAG TAATGAGAAG AAGGCCAAGA AGGTACGTTT CTACCGCAAT 
GGGGACCGTT ACTTCAAGGG GATTGTGTAC GCTGTTTCTT CTGACCGTTT TCGTAGTTTT 
GATGCATTGC TGGCTGACCT GACTCGATCC TTGTCCGACA ACATCAACCT GCCTCAGGGA 
GTGCGCTACA TTTACACTAT TGATGGATCC AGAAAGATTG GAAGCATGGA TGAACTGGAA 
GAAGGGGAAA GCTATGTCTG TTCCTCAGAC AACTTCTTTA AAAAGGTTGA GTATACCAAG 
AATGTCAATC CCAACTGGTC GGTCAATGTA AAGACATCAG CCAACATGAA AGCGCCCCAG 
TCCTTGGCTA GCAGCAACAG TGCTCAAGCC AGAGAGAACA AGGACTTCGT GCGCCCCAAA 
CTTGTGACCA TCATTCGCAG TGGGGTGAAG CCTCGGAAGG CGGTGCGTGT GCTTCTCAAC 
AAGAAAACAG CCCACTCTTT CGAGCAGGTC CTCACTGACA TCACAGAAGC GATCAAACTG 
GAAACCGGAG TTGTCAAAAA ACTCTATACC CTTGATGGAA AGCAGGTCAC CTGTCTCCAT 
GATTTCTTTG GTGATGATGA TGTGTTCATT GCTTGTGGCC CTGAAAAGTT CCGCTATGCT 
CAAGATGACT TCTCCTTGGA TGAGAATGAA TGCAGAGTCA TGAAAGGGAA TCCATCTGCC 
ACAGCTGGCC CAAAAGCTTC CCCAACACCT CAAAAGACAT CTGCTAAAAG CCCAGGCCCA 
ATGCGCCGCA GCAAGTCTCC AGCTGACTCA GGTAACGACC AAGACGCAAA TGGAACTTCT 
AGCAGTCAGC TCTCAACACC TAAGTCGAAG CAGTCTCCTA TCTCTACACC CACAAGCCCT 
GGAAGTCTGC GGAAGCACAA GGTAGACTTG TACCTGCCAC TGTCATTGGA TGACTCTGAT 
TCACTTGGCG ATTCCATGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006257444.1
CDS	192..1292
Translation	MELDFGHFDERDKASRNMRGSRMNGLPSPTHSAHCSFYRTRTLQALSNEKKAKKVRFYRNGDRYFKGIVYAVSSDRFRSFDALLADLTRSLSDNINLPQGVRYIYTIDGSRKIGSMDELEEGESYVCSSDNFFKKVEYTKNVNPNWSVNVKTSANMKAPQSLASSNSAQARENKDFVRPKLVTIIRSGVKPRKAVRVLLNKKTAHSFEQVLTDITEAIKLETGVVKKLYTLDGKQVTCLHDFFGDDDVFIACGPEKFRYAQDDFSLDENECRVMKGNPSATAGPKASPTPQKTSAKSPGPMRRSKSPADSGNDQDANGTSSSQLSTPKSKQSPISTPTSPGSLRKHKVDLYLPLSLDDSDSLGDSM

Target ORF information:

RefSeq Version	XM_006257382.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus doublecortin (Dcx), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006257382.2

ORF Insert Sequence:

ATGGAACTTG ATTTTGGACA TTTTGACGAA CGAGACAAAG CATCTAGAAA TATGAGAGGG 
TCACGGATGA ATGGACTTCC AAGTCCCACT CATAGTGCTC ACTGTAGCTT CTACAGAACC 
AGAACCTTGC AGGCATTAAG TAATGAGAAG AAGGCCAAGA AGGTACGTTT CTACCGCAAT 
GGGGACCGTT ACTTCAAGGG GATTGTGTAC GCTGTTTCTT CTGACCGTTT TCGTAGTTTT 
GATGCATTGC TGGCTGACCT GACTCGATCC TTGTCCGACA ACATCAACCT GCCTCAGGGA 
GTGCGCTACA TTTACACTAT TGATGGATCC AGAAAGATTG GAAGCATGGA TGAACTGGAA 
GAAGGGGAAA GCTATGTCTG TTCCTCAGAC AACTTCTTTA AAAAGGTTGA GTATACCAAG 
AATGTCAATC CCAACTGGTC GGTCAATGTA AAGACATCAG CCAACATGAA AGCGCCCCAG 
TCCTTGGCTA GCAGCAACAG TGCTCAAGCC AGAGAGAACA AGGACTTCGT GCGCCCCAAA 
CTTGTGACCA TCATTCGCAG TGGGGTGAAG CCTCGGAAGG CGGTGCGTGT GCTTCTCAAC 
AAGAAAACAG CCCACTCTTT CGAGCAGGTC CTCACTGACA TCACAGAAGC GATCAAACTG 
GAAACCGGAG TTGTCAAAAA ACTCTATACC CTTGATGGAA AGCAGGTCAC CTGTCTCCAT 
GATTTCTTTG GTGATGATGA TGTGTTCATT GCTTGTGGCC CTGAAAAGTT CCGCTATGCT 
CAAGATGACT TCTCCTTGGA TGAGAATGAA TGCAGAGTCA TGAAAGGGAA TCCATCTGCC 
ACAGCTGGCC CAAAAGCTTC CCCAACACCT CAAAAGACAT CTGCTAAAAG CCCAGGCCCA 
ATGCGCCGCA GCAAGTCTCC AGCTGACTCA GGTAACGACC AAGACGCAAA TGGAACTTCT 
AGCAGTCAGC TCTCAACACC TAAGTCGAAG CAGTCTCCTA TCTCTACACC CACAAGCCCT 
GGAAGTCTGC GGAAGCACAA GGTAGACTTG TACCTGCCAC TGTCATTGGA TGACTCTGAT 
TCACTTGGCG ATTCCATGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dcx ( XM_006257383.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006257445.1 Rattus norvegicus doublecortin (Dcx), transcript variant X5, mRNA.

Price & Availability↑

CloneID	ORa40152
Clone ID Related Accession (Same CDS sequence)	XM_006257383.3
Accession Version	XM_006257383.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1083bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	neuronal migration protein doublecortin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005120.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006257383.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAACTTG ATTTTGGACA TTTTGACGAA CGAGACAAAG CATCTAGAAA TATGAGAGGG 
TCACGGATGA ATGGACTTCC AAGTCCCACT CATAGTGCTC ACTGTAGCTT CTACAGAACC 
AGAACCTTGC AGGCATTAAG TAATGAGAAG AAGGCCAAGA AGGTACGTTT CTACCGCAAT 
GGGGACCGTT ACTTCAAGGG GATTGTGTAC GCTGTTTCTT CTGACCGTTT TCGTAGTTTT 
GATGCATTGC TGGCTGACCT GACTCGATCC TTGTCCGACA ACATCAACCT GCCTCAGGGA 
GTGCGCTACA TTTACACTAT TGATGGATCC AGAAAGATTG GAAGCATGGA TGAACTGGAA 
GAAGGGGAAA GCTATGTCTG TTCCTCAGAC AACTTCTTTA AAAAGGTTGA GTATACCAAG 
AATGTCAATC CCAACTGGTC GGTCAATGTA AAGACATCAG CCAACATGAA AGCGCCCCAG 
TCCTTGGCTA GCAGCAACAG TGCTCAAGCC AGAGAGAACA AGGACTTCGT GCGCCCCAAA 
CTTGTGACCA TCATTCGCAG TGGGGTGAAG CCTCGGAAGG CGGTGCGTGT GCTTCTCAAC 
AAGAAAACAG CCCACTCTTT CGAGCAGGTC CTCACTGACA TCACAGAAGC GATCAAACTG 
GAAACCGGAG TTGTCAAAAA ACTCTATACC CTTGATGGAA AGCAGGTCAC CTGTCTCCAT 
GATTTCTTTG GTGATGATGA TGTGTTCATT GCTTGTGGCC CTGAAAAGTT CCGCTATGCT 
CAAGATGACT TCTCCTTGGA TGAGAATGAA TGCAGAGTCA TGAAAGGGAA TCCATCTGCC 
ACAGCTGGCC CAAAAGCTTC CCCAACACCT CAAAAGACAT CTGCTAAAAG CCCAGGCCCA 
ATGCGCCGCA GCAAGTCTCC AGCTGACTCA GCAAATGGAA CTTCTAGCAG TCAGCTCTCA 
ACACCTAAGT CGAAGCAGTC TCCTATCTCT ACACCCACAA GCCCTGGAAG TCTGCGGAAG 
CACAAGGACT TGTACCTGCC ACTGTCATTG GATGACTCTG ATTCACTTGG CGATTCCATG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006257445.1
CDS	283..1365
Translation	MELDFGHFDERDKASRNMRGSRMNGLPSPTHSAHCSFYRTRTLQALSNEKKAKKVRFYRNGDRYFKGIVYAVSSDRFRSFDALLADLTRSLSDNINLPQGVRYIYTIDGSRKIGSMDELEEGESYVCSSDNFFKKVEYTKNVNPNWSVNVKTSANMKAPQSLASSNSAQARENKDFVRPKLVTIIRSGVKPRKAVRVLLNKKTAHSFEQVLTDITEAIKLETGVVKKLYTLDGKQVTCLHDFFGDDDVFIACGPEKFRYAQDDFSLDENECRVMKGNPSATAGPKASPTPQKTSAKSPGPMRRSKSPADSANGTSSSQLSTPKSKQSPISTPTSPGSLRKHKDLYLPLSLDDSDSLGDSM

Target ORF information:

RefSeq Version	XM_006257383.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus doublecortin (Dcx), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006257383.3

ORF Insert Sequence:

ATGGAACTTG ATTTTGGACA TTTTGACGAA CGAGACAAAG CATCTAGAAA TATGAGAGGG 
TCACGGATGA ATGGACTTCC AAGTCCCACT CATAGTGCTC ACTGTAGCTT CTACAGAACC 
AGAACCTTGC AGGCATTAAG TAATGAGAAG AAGGCCAAGA AGGTACGTTT CTACCGCAAT 
GGGGACCGTT ACTTCAAGGG GATTGTGTAC GCTGTTTCTT CTGACCGTTT TCGTAGTTTT 
GATGCATTGC TGGCTGACCT GACTCGATCC TTGTCCGACA ACATCAACCT GCCTCAGGGA 
GTGCGCTACA TTTACACTAT TGATGGATCC AGAAAGATTG GAAGCATGGA TGAACTGGAA 
GAAGGGGAAA GCTATGTCTG TTCCTCAGAC AACTTCTTTA AAAAGGTTGA GTATACCAAG 
AATGTCAATC CCAACTGGTC GGTCAATGTA AAGACATCAG CCAACATGAA AGCGCCCCAG 
TCCTTGGCTA GCAGCAACAG TGCTCAAGCC AGAGAGAACA AGGACTTCGT GCGCCCCAAA 
CTTGTGACCA TCATTCGCAG TGGGGTGAAG CCTCGGAAGG CGGTGCGTGT GCTTCTCAAC 
AAGAAAACAG CCCACTCTTT CGAGCAGGTC CTCACTGACA TCACAGAAGC GATCAAACTG 
GAAACCGGAG TTGTCAAAAA ACTCTATACC CTTGATGGAA AGCAGGTCAC CTGTCTCCAT 
GATTTCTTTG GTGATGATGA TGTGTTCATT GCTTGTGGCC CTGAAAAGTT CCGCTATGCT 
CAAGATGACT TCTCCTTGGA TGAGAATGAA TGCAGAGTCA TGAAAGGGAA TCCATCTGCC 
ACAGCTGGCC CAAAAGCTTC CCCAACACCT CAAAAGACAT CTGCTAAAAG CCCAGGCCCA 
ATGCGCCGCA GCAAGTCTCC AGCTGACTCA GCAAATGGAA CTTCTAGCAG TCAGCTCTCA 
ACACCTAAGT CGAAGCAGTC TCCTATCTCT ACACCCACAA GCCCTGGAAG TCTGCGGAAG 
CACAAGGACT TGTACCTGCC ACTGTCATTG GATGACTCTG ATTCACTTGG CGATTCCATG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dcx ( XM_006257384.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006257446.1 Rattus norvegicus doublecortin (Dcx), transcript variant X6, mRNA.

Price & Availability↑

CloneID	ORa40153
Clone ID Related Accession (Same CDS sequence)	XM_006257384.3
Accession Version	XM_006257384.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 975bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	neuronal migration protein doublecortin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005120.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006257384.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAACTTG ATTTTGGACA TTTTGACGAA CGAGACAAAG CATCTAGAAA TATGAGAGGG 
TCACGGATGA ATGGACTTCC AAGTCCCACT CATAGTGCTC ACTGTAGCTT CTACAGAACC 
AGAACCTTGC AGGCATTAAG TAATGAGAAG AAGGCCAAGA AGGTACGTTT CTACCGCAAT 
GGGGACCGTT ACTTCAAGGG GATTGTGTAC GCTGTTTCTT CTGACCGTTT TCGTAGTTTT 
GATGCATTGC TGGCTGACCT GACTCGATCC TTGTCCGACA ACATCAACCT GCCTCAGGGA 
GTGCGCTACA TTTACACTAT TGATGGATCC AGAAAGATTG GAAGCATGGA TGAACTGGAA 
GAAGGGGAAA GCTATGTCTG TTCCTCAGAC AACTTCTTTA AAAAGGTTGA GTATACCAAG 
AATGTCAATC CCAACTGGTC GGTCAATGTA AAGACATCAG CCAACATGAA AGCGCCCCAG 
TCCTTGGCTA GCAGCAACAG TGCTCAAGCC AGAGAGAACA AGGACTTCGT GCGCCCCAAA 
CTTGTGACCA TCATTCGCAG TGGGGTGAAG CCTCGGAAGG CGGTGCGTGT GCTTCTCAAC 
AAGAAAACAG CCCACTCTTT CGAGCAGGTC CTCACTGACA TCACAGAAGC GATCAAACTG 
GAAACCGGAG TTGTCAAAAA ACTCTATACC CTTGATGGAA AGCAGGTCAC CTGTCTCCAT 
GATTTCTTTG GTGATGATGA TGTGTTCATT GCTTGTGGCC CTGAAAAGTT CCGCTATGCT 
CAAGATGACT TCTCCTTGGA TGAGAATGAA TGCAGAGTCA TGAAAGGGAA TCCATCTGCC 
ACAGCTGGCC CAAAAGCTTC CCCAACACCT CAAAAGACAT CTGCTAAAAG CCCAGGCCCA 
ATGCGCCGCA GCAAGTCTCC AGCTGACTCA GGTAACGACC AAGACGGACT TGTACCTGCC 
ACTGTCATTG GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006257446.1
CDS	282..1256
Translation	MELDFGHFDERDKASRNMRGSRMNGLPSPTHSAHCSFYRTRTLQALSNEKKAKKVRFYRNGDRYFKGIVYAVSSDRFRSFDALLADLTRSLSDNINLPQGVRYIYTIDGSRKIGSMDELEEGESYVCSSDNFFKKVEYTKNVNPNWSVNVKTSANMKAPQSLASSNSAQARENKDFVRPKLVTIIRSGVKPRKAVRVLLNKKTAHSFEQVLTDITEAIKLETGVVKKLYTLDGKQVTCLHDFFGDDDVFIACGPEKFRYAQDDFSLDENECRVMKGNPSATAGPKASPTPQKTSAKSPGPMRRSKSPADSGNDQDGLVPATVIG

Target ORF information:

RefSeq Version	XM_006257384.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus doublecortin (Dcx), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006257384.3

ORF Insert Sequence:

ATGGAACTTG ATTTTGGACA TTTTGACGAA CGAGACAAAG CATCTAGAAA TATGAGAGGG 
TCACGGATGA ATGGACTTCC AAGTCCCACT CATAGTGCTC ACTGTAGCTT CTACAGAACC 
AGAACCTTGC AGGCATTAAG TAATGAGAAG AAGGCCAAGA AGGTACGTTT CTACCGCAAT 
GGGGACCGTT ACTTCAAGGG GATTGTGTAC GCTGTTTCTT CTGACCGTTT TCGTAGTTTT 
GATGCATTGC TGGCTGACCT GACTCGATCC TTGTCCGACA ACATCAACCT GCCTCAGGGA 
GTGCGCTACA TTTACACTAT TGATGGATCC AGAAAGATTG GAAGCATGGA TGAACTGGAA 
GAAGGGGAAA GCTATGTCTG TTCCTCAGAC AACTTCTTTA AAAAGGTTGA GTATACCAAG 
AATGTCAATC CCAACTGGTC GGTCAATGTA AAGACATCAG CCAACATGAA AGCGCCCCAG 
TCCTTGGCTA GCAGCAACAG TGCTCAAGCC AGAGAGAACA AGGACTTCGT GCGCCCCAAA 
CTTGTGACCA TCATTCGCAG TGGGGTGAAG CCTCGGAAGG CGGTGCGTGT GCTTCTCAAC 
AAGAAAACAG CCCACTCTTT CGAGCAGGTC CTCACTGACA TCACAGAAGC GATCAAACTG 
GAAACCGGAG TTGTCAAAAA ACTCTATACC CTTGATGGAA AGCAGGTCAC CTGTCTCCAT 
GATTTCTTTG GTGATGATGA TGTGTTCATT GCTTGTGGCC CTGAAAAGTT CCGCTATGCT 
CAAGATGACT TCTCCTTGGA TGAGAATGAA TGCAGAGTCA TGAAAGGGAA TCCATCTGCC 
ACAGCTGGCC CAAAAGCTTC CCCAACACCT CAAAAGACAT CTGCTAAAAG CCCAGGCCCA 
ATGCGCCGCA GCAAGTCTCC AGCTGACTCA GGTAACGACC AAGACGGACT TGTACCTGCC 
ACTGTCATTG GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dcx ( XM_006257386.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006257448.1 Rattus norvegicus doublecortin (Dcx), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ORa40154
Clone ID Related Accession (Same CDS sequence)	XM_006257386.3
Accession Version	XM_006257386.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1086bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	neuronal migration protein doublecortin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005120.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006257386.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAACTTG ATTTTGGACA TTTTGACGAA CGAGACAAAG CATCTAGAAA TATGAGAGGG 
TCACGGATGA ATGGACTTCC AAGTCCCACT CATAGTGCTC ACTGTAGCTT CTACAGAACC 
AGAACCTTGC AGGCATTAAG TAATGAGAAG AAGGCCAAGA AGGTACGTTT CTACCGCAAT 
GGGGACCGTT ACTTCAAGGG GATTGTGTAC GCTGTTTCTT CTGACCGTTT TCGTAGTTTT 
GATGCATTGC TGGCTGACCT GACTCGATCC TTGTCCGACA ACATCAACCT GCCTCAGGGA 
GTGCGCTACA TTTACACTAT TGATGGATCC AGAAAGATTG GAAGCATGGA TGAACTGGAA 
GAAGGGGAAA GCTATGTCTG TTCCTCAGAC AACTTCTTTA AAAAGGTTGA GTATACCAAG 
AATGTCAATC CCAACTGGTC GGTCAATGTA AAGACATCAG CCAACATGAA AGCGCCCCAG 
TCCTTGGCTA GCAGCAACAG TGCTCAAGCC AGAGAGAACA AGGACTTCGT GCGCCCCAAA 
CTTGTGACCA TCATTCGCAG TGGGGTGAAG CCTCGGAAGG CGGTGCGTGT GCTTCTCAAC 
AAGAAAACAG CCCACTCTTT CGAGCAGGTC CTCACTGACA TCACAGAAGC GATCAAACTG 
GAAACCGGAG TTGTCAAAAA ACTCTATACC CTTGATGGAA AGCAGGTCAC CTGTCTCCAT 
GATTTCTTTG GTGATGATGA TGTGTTCATT GCTTGTGGCC CTGAAAAGTT CCGCTATGCT 
CAAGATGACT TCTCCTTGGA TGAGAATGAA TGCAGAGTCA TGAAAGGGAA TCCATCTGCC 
ACAGCTGGCC CAAAAGCTTC CCCAACACCT CAAAAGACAT CTGCTAAAAG CCCAGGCCCA 
ATGCGCCGCA GCAAGTCTCC AGCTGACTCA GCAAATGGAA CTTCTAGCAG TCAGCTCTCA 
ACACCTAAGT CGAAGCAGTC TCCTATCTCT ACACCCACAA GCCCTGGAAG TCTGCGGAAG 
CACAAGGTAG ACTTGTACCT GCCACTGTCA TTGGATGACT CTGATTCACT TGGCGATTCC 
ATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006257448.1
CDS	283..1368
Translation	MELDFGHFDERDKASRNMRGSRMNGLPSPTHSAHCSFYRTRTLQALSNEKKAKKVRFYRNGDRYFKGIVYAVSSDRFRSFDALLADLTRSLSDNINLPQGVRYIYTIDGSRKIGSMDELEEGESYVCSSDNFFKKVEYTKNVNPNWSVNVKTSANMKAPQSLASSNSAQARENKDFVRPKLVTIIRSGVKPRKAVRVLLNKKTAHSFEQVLTDITEAIKLETGVVKKLYTLDGKQVTCLHDFFGDDDVFIACGPEKFRYAQDDFSLDENECRVMKGNPSATAGPKASPTPQKTSAKSPGPMRRSKSPADSANGTSSSQLSTPKSKQSPISTPTSPGSLRKHKVDLYLPLSLDDSDSLGDSM

Target ORF information:

RefSeq Version	XM_006257386.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus doublecortin (Dcx), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006257386.3

ORF Insert Sequence:

ATGGAACTTG ATTTTGGACA TTTTGACGAA CGAGACAAAG CATCTAGAAA TATGAGAGGG 
TCACGGATGA ATGGACTTCC AAGTCCCACT CATAGTGCTC ACTGTAGCTT CTACAGAACC 
AGAACCTTGC AGGCATTAAG TAATGAGAAG AAGGCCAAGA AGGTACGTTT CTACCGCAAT 
GGGGACCGTT ACTTCAAGGG GATTGTGTAC GCTGTTTCTT CTGACCGTTT TCGTAGTTTT 
GATGCATTGC TGGCTGACCT GACTCGATCC TTGTCCGACA ACATCAACCT GCCTCAGGGA 
GTGCGCTACA TTTACACTAT TGATGGATCC AGAAAGATTG GAAGCATGGA TGAACTGGAA 
GAAGGGGAAA GCTATGTCTG TTCCTCAGAC AACTTCTTTA AAAAGGTTGA GTATACCAAG 
AATGTCAATC CCAACTGGTC GGTCAATGTA AAGACATCAG CCAACATGAA AGCGCCCCAG 
TCCTTGGCTA GCAGCAACAG TGCTCAAGCC AGAGAGAACA AGGACTTCGT GCGCCCCAAA 
CTTGTGACCA TCATTCGCAG TGGGGTGAAG CCTCGGAAGG CGGTGCGTGT GCTTCTCAAC 
AAGAAAACAG CCCACTCTTT CGAGCAGGTC CTCACTGACA TCACAGAAGC GATCAAACTG 
GAAACCGGAG TTGTCAAAAA ACTCTATACC CTTGATGGAA AGCAGGTCAC CTGTCTCCAT 
GATTTCTTTG GTGATGATGA TGTGTTCATT GCTTGTGGCC CTGAAAAGTT CCGCTATGCT 
CAAGATGACT TCTCCTTGGA TGAGAATGAA TGCAGAGTCA TGAAAGGGAA TCCATCTGCC 
ACAGCTGGCC CAAAAGCTTC CCCAACACCT CAAAAGACAT CTGCTAAAAG CCCAGGCCCA 
ATGCGCCGCA GCAAGTCTCC AGCTGACTCA GCAAATGGAA CTTCTAGCAG TCAGCTCTCA 
ACACCTAAGT CGAAGCAGTC TCCTATCTCT ACACCCACAA GCCCTGGAAG TCTGCGGAAG 
CACAAGGTAG ACTTGTACCT GCCACTGTCA TTGGATGACT CTGATTCACT TGGCGATTCC 
ATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A novel DCX missense mutation in a family with X-linked lissencephaly and subcortical band heterotopia syndrome inherited from a low-level somatic mosaic mother: Genetic and functional studies.
European journal of paediatric neurology : EJPN : official journal of the European Paediatric Neurology Society20(5)788-94(2016 Sep)
Tsai MH,Kuo PW,Myers CT,Li SW,Lin WC,Fu TY,Chang HY,Mefford HC,Chang YC,Tsai JW

[EXPRESSION OF DOUBLECORTIN AND NeuN IN THE DEVELOPING CEREBELLAR NEURONS IN RAT].
Morfologiia (Saint Petersburg, Russia)149(1)38-42(2016)
Zimatkin SM,Karniushko OA

Disruption of Visc-2, a Brain-Expressed Conserved Long Noncoding RNA, Does Not Elicit an Overt Anatomical or Behavioral Phenotype.
Cerebral cortex (New York, N.Y. : 1991)25(10)3572-85(2015 Oct)
Oliver PL,Chodroff RA,Gosal A,Edwards B,Cheung AF,Gomez-Rodriguez J,Elliot G,Garrett LJ,Lickiss T,Szele F,Green ED,Moln?r Z,Ponting CP

Time course of spinal doublecortin expression in developing rat and porcine spinal cord: implication in in vivo neural precursor grafting studies.
Cellular and molecular neurobiology35(1)57-70(2015 Jan)
Juhasova J,Juhas S,Hruska-Plochan M,Dolezalova D,Holubova M,Strnadel J,Marsala S,Motlik J,Marsala M

Protein kinase LKB1 regulates polarized dendrite formation of adult hippocampal newborn neurons.
Proceedings of the National Academy of Sciences of the United States of America111(1)469-74(2014 Jan)
Huang W,She L,Chang XY,Yang RR,Wang L,Ji HB,Jiao JW,Poo MM

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Dcx cDNA ORF clone, Rattus norvegicus(Norway rat)

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ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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