Dffb cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Dffb
Entrez Gene ID	84359
Full Name	DNA fragmentation factor subunit beta
Synonyms	Cad
General protein information	Preferred Names DNA fragmentation factor subunit beta Names DNA fragmentation factor subunit beta DFF-40 DNA fragmentation factor 40 kDa subunit DNA fragmentation factor, 40 kD, beta polypeptide (caspase-activated DNase) DNA fragmentation factor, 40kDa, beta polypeptide (caspase-activated DNase) DNA fragmentation factor, beta polypeptide (caspase-activated DNase) DNA fragmentation factor, beta subunit caspase-activated DNase caspase-activated deoxyribonuclease
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 5 Map Location: 5q36
Summary	endogenous endonuclease; mediates caspase-3-dependent internucleosomal DNA degradation and related nuclear alterations [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_053362.1	NP_445814.1	DNA fragmentation factor subunit beta

Pathways from BioSystems

Homologies

Bos taurus (cattle)	DFFB	NP_001030281.1
Gallus gallus (chicken)	DFFB	NP_989616.1
Homo sapiens (human)	DFFB	NP_004393.1
Canis lupus familiaris (dog)	DFFB	XP_546743.2
Mus musculus (house mouse)	Dffb	NP_031885.3
Rattus norvegicus (Norway rat)	Dffb	NP_445814.1
Danio rerio (zebrafish)	dffb	NP_919385.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009681	XP_318738.4
Xenopus tropicalis (tropical clawed frog)	dffb	NP_001120636.1
Pan troglodytes (chimpanzee)	DFFB	XP_001153262.1
Drosophila melanogaster (fruit fly)	Drep-4	NP_609631.1

Gene Ontology
Bibliography

Related articles in PubMed

Apoptotic DNA degradation into oligonucleosomal fragments, but not apoptotic nuclear morphology, relies on a cytosolic pool of DFF40/CAD endonuclease.
Iglesias-Guimarais V, Gil-Gui?on E, Gabernet G, Garc?a-Belinch?n M, S?nchez-Osuna M, Casanelles E, Comella JX, Yuste VJ
The Journal of biological chemistry287(10)7766-79(2012 Mar)

Histone H1 subtype preferences of DFF40 and possible nuclear localization of DFF40/45 in normal and trichostatin A-treated NB4 leukemic cells.
Ninios YP, Sekeri-Pataryas KE, Sourlingas TG
Apoptosis : an international journal on programmed cell death15(2)128-38(2010 Feb)

Fifty C-terminal amino acid residues are necessary for the chaperone activity of DFF45 but not for the inhibition of DFF40.
Park HH
BMB reports42(11)713-8(2009 Nov)

AMPA-induced excitotoxicity increases nuclear levels of CAD, endonuclease G, and acinus and induces chromatin condensation in rat hippocampal pyramidal neurons.
Henne WM, Oomman S, Attridge J, Finckbone V, Coates P, Bliss R, Strahlendorf H, Strahlendorf J
Cellular and molecular neurobiology26(3)321-39(2006 May)

Nuclear Akt associates with PKC-phosphorylated Ebp1, preventing DNA fragmentation by inhibition of caspase-activated DNase.
Ahn JY, Liu X, Liu Z, Pereira L, Cheng D, Peng J, Wade PA, Hamburger AW, Ye K
The EMBO journal25(10)2083-95(2006 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data indicate increases in the nuclear levels of caspase-activated acinus and DNase and Endonuclease G (a caspase-independent endonuclease) in CA1 and CA3 pyramidal cell nuclei with different temporal patterns following an AMPA-insult.
Title: AMPA-induced excitotoxicity increases nuclear levels of CAD, endonuclease G, and acinus and induces chromatin condensation in rat hippocampal pyramidal neurons.

Results suggest that Ebp1 contributes to inhibition of DNA fragmentation by caspase-activated DNase, and that nuclear Akt might contribute to suppressing apoptosis through interaction with Ebp1.
Title: Nuclear Akt associates with PKC-phosphorylated Ebp1, preventing DNA fragmentation by inhibition of caspase-activated DNase.

that variations in TP53 and hCAD genes modulate the age of onset of Huntington disease .
Title: Modulation of age at onset of Huntington disease patients by variations in TP53 and human caspase activated DNase (hCAD) genes.

DNA fragmentation in neuronal apoptosis is catalyzed by either CAD or DNase gamma depending on the differentiation state
Title: Differential DNases are selectively used in neuronal apoptosis depending on the differentiation state.

The following Dffb gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dffb cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa09355	NM_053362.1 Latest version!	Rattus norvegicus DNA fragmentation factor subunit beta (Dffb), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dffb ( NM_053362.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_445814.1 Rattus norvegicus DNA fragmentation factor subunit beta (Dffb), mRNA.

Price & Availability↑

CloneID	ORa09355
Clone ID Related Accession (Same CDS sequence)	NM_053362.1
Accession Version	NM_053362.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1050bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Rattus norvegicus(Norway rat)
Product	DNA fragmentation factor subunit beta
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF136598.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF136598.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01906353 [ECO:0000348] ##Evidence-Data-END##

ATGTGCGCGG TGCTCCCCCA ACCCAAATGC GTCAAGTTGC GAGCTCTACA CAGCTCGTGC 
AAGTTCGGCG TGGCGGCCCG GAGCTGCCAG GAGCTGCTGC GCAAGGGTTG TATCCGCTTC 
CAGCTCCCGG TGCCCGGCTC CCGGCTGTGC ATGTACGAAG ATGGCACGGA GGTGACCGAC 
GACTGCTTCC CGAGCCTTCC CAACGACTCT GAGCTCCTAT TGCTCACCGC TGGAGAGACC 
TGGCACGGCT ATGTGAGTGA CATCACACGT CTCCTCAGTG TGTTTAACGA GCCACATGCC 
GGGGTCATCC AGGCTGCGCG GCAACTGCTG TCAGATGAGC AGGCCCCACT GAGGCAGAAG 
CTGCTGGCCG ACCTTCTGCA TCATGTGAGC CAGAATATTA CTGCAGAGAC CCGGGAACAG 
GACCCATCCT GGTTTGAAGG TTTGGAGTCT CGATTCAGGA ATAAGTCTGG CTACCTGAGA 
TACAGCTGTG AGAGCCGGAT CCGGGGTTAC CTGAGAGAGG TGAGCGCTTA CATCTCAATG 
GTGGATGCAG CAGCCCGGGA AGAGTACCTG CGGGTCCTCA GCTCCATGTG CCACAAGCTC 
AAGTCCGTGC AGTACAACGG CAGCTATTTC GACAGAGGTG CAGAGGCCAG CAGCCGCCTT 
TGTACCCCAG AAGGCTGGTT CTCCTGCCAG GGCCCCTTTG ACTTGGAGAG CTGTCTCTCC 
AAACACTCCA TCAACCCCTA TGGCAACAGA GAGAGCCGGA TCCTCTTCAG TACCTGGAAC 
CTGGACCATA TAATAGAGAA GAAGCGCACC GTGGTGCCCA CGCTGGCTGA AGCAATCCAG 
GACGGGAGGG AAGTGAACTG GGAGTACTTC TACAGCCTGC TCTTCACTGC CGAGAACCTG 
AAGCTAGTGC ACATCGCCTG CCACAAGAAG ACCACACACA AGCTGCAGTG CGACCGCAGC 
AGGATCTATC GGCCTCAGAC AGGATCCAGG AGGAAGCAGC CCCCTCGGAA GCAGCCCCCT 
CGGAAGCGCC CCCCTCGGAA ACGCCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_445814.1
CDS	15..1064
Translation	MCAVLPQPKCVKLRALHSSCKFGVAARSCQELLRKGCIRFQLPVPGSRLCMYEDGTEVTDDCFPSLPNDSELLLLTAGETWHGYVSDITRLLSVFNEPHAGVIQAARQLLSDEQAPLRQKLLADLLHHVSQNITAETREQDPSWFEGLESRFRNKSGYLRYSCESRIRGYLREVSAYISMVDAAAREEYLRVLSSMCHKLKSVQYNGSYFDRGAEASSRLCTPEGWFSCQGPFDLESCLSKHSINPYGNRESRILFSTWNLDHIIEKKRTVVPTLAEAIQDGREVNWEYFYSLLFTAENLKLVHIACHKKTTHKLQCDRSRIYRPQTGSRRKQPPRKQPPRKRPPRKRQ

Target ORF information:

RefSeq Version	NM_053362.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus DNA fragmentation factor subunit beta (Dffb), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_053362.1

ORF Insert Sequence:

ATGTGCGCGG TGCTCCCCCA ACCCAAATGC GTCAAGTTGC GAGCTCTACA CAGCTCGTGC 
AAGTTCGGCG TGGCGGCCCG GAGCTGCCAG GAGCTGCTGC GCAAGGGTTG TATCCGCTTC 
CAGCTCCCGG TGCCCGGCTC CCGGCTGTGC ATGTACGAAG ATGGCACGGA GGTGACCGAC 
GACTGCTTCC CGAGCCTTCC CAACGACTCT GAGCTCCTAT TGCTCACCGC TGGAGAGACC 
TGGCACGGCT ATGTGAGTGA CATCACACGT CTCCTCAGTG TGTTTAACGA GCCACATGCC 
GGGGTCATCC AGGCTGCGCG GCAACTGCTG TCAGATGAGC AGGCCCCACT GAGGCAGAAG 
CTGCTGGCCG ACCTTCTGCA TCATGTGAGC CAGAATATTA CTGCAGAGAC CCGGGAACAG 
GACCCATCCT GGTTTGAAGG TTTGGAGTCT CGATTCAGGA ATAAGTCTGG CTACCTGAGA 
TACAGCTGTG AGAGCCGGAT CCGGGGTTAC CTGAGAGAGG TGAGCGCTTA CATCTCAATG 
GTGGATGCAG CAGCCCGGGA AGAGTACCTG CGGGTCCTCA GCTCCATGTG CCACAAGCTC 
AAGTCCGTGC AGTACAACGG CAGCTATTTC GACAGAGGTG CAGAGGCCAG CAGCCGCCTT 
TGTACCCCAG AAGGCTGGTT CTCCTGCCAG GGCCCCTTTG ACTTGGAGAG CTGTCTCTCC 
AAACACTCCA TCAACCCCTA TGGCAACAGA GAGAGCCGGA TCCTCTTCAG TACCTGGAAC 
CTGGACCATA TAATAGAGAA GAAGCGCACC GTGGTGCCCA CGCTGGCTGA AGCAATCCAG 
GACGGGAGGG AAGTGAACTG GGAGTACTTC TACAGCCTGC TCTTCACTGC CGAGAACCTG 
AAGCTAGTGC ACATCGCCTG CCACAAGAAG ACCACACACA AGCTGCAGTG CGACCGCAGC 
AGGATCTATC GGCCTCAGAC AGGATCCAGG AGGAAGCAGC CCCCTCGGAA GCAGCCCCCT 
CGGAAGCGCC CCCCTCGGAA ACGCCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Apoptotic DNA degradation into oligonucleosomal fragments, but not apoptotic nuclear morphology, relies on a cytosolic pool of DFF40/CAD endonuclease.
The Journal of biological chemistry287(10)7766-79(2012 Mar)
Iglesias-Guimarais V,Gil-Gui?on E,Gabernet G,Garc?a-Belinch?n M,S?nchez-Osuna M,Casanelles E,Comella JX,Yuste VJ

Histone H1 subtype preferences of DFF40 and possible nuclear localization of DFF40/45 in normal and trichostatin A-treated NB4 leukemic cells.
Apoptosis : an international journal on programmed cell death15(2)128-38(2010 Feb)
Ninios YP,Sekeri-Pataryas KE,Sourlingas TG

Fifty C-terminal amino acid residues are necessary for the chaperone activity of DFF45 but not for the inhibition of DFF40.
BMB reports42(11)713-8(2009 Nov)
Park HH

AMPA-induced excitotoxicity increases nuclear levels of CAD, endonuclease G, and acinus and induces chromatin condensation in rat hippocampal pyramidal neurons.
Cellular and molecular neurobiology26(3)321-39(2006 May)
Henne WM,Oomman S,Attridge J,Finckbone V,Coates P,Bliss R,Strahlendorf H,Strahlendorf J

Nuclear Akt associates with PKC-phosphorylated Ebp1, preventing DNA fragmentation by inhibition of caspase-activated DNase.
The EMBO journal25(10)2083-95(2006 May)
Ahn JY,Liu X,Liu Z,Pereira L,Cheng D,Peng J,Wade PA,Hamburger AW,Ye K

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Dffb cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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